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Pulp stem cells

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https://www.readbyqxmd.com/read/29341308/stiffness-regulates-the-proliferation-and-osteogenic-odontogenic-differentiation-of-human-dental-pulp-stem-cells-via-the-wnt-signalling-pathway
#1
Nanxin Liu, Mi Zhou, Qi Zhang, Tao Zhang, Taoran Tian, Quanquan Ma, Changyue Xue, Shiyu Lin, Xiaoxiao Cai
OBJECTIVES: Researches showed that stiffness of the extracellular matrix can affect the differentiation of many stem cells. Dental pulp stem cells (DPSCs) are a promising type of adult stem cell. However, we know little about whether and how the behaviour of DPSCs is influenced by stiffness. MATERIALS AND METHODS: We carried out a study that cultured DPSCs on tunable elasticity polydimethylsiloxane substrates to investigate the influence on morphology, proliferation, osteogenic/odontogenic differentiation and its possible mechanism...
January 17, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29327447/itraq-based-proteomic-analysis-explore-the-influence-of-hypoxia-on-the-proteome-of-dental-pulp-stem-cells-under-3d-culture
#2
Lei Dou, Qifang Yan, Panpan Liang, Pengfei Zhou, Yan Zhang, Ping Ji
Various approaches are used to improve the efficacy of mesenchymal stem cells(MSCs) therapy. Hypoxic preconditioning is one of the most commonly-applied strategy prior to MSCs transplantation for enhancing the therapeutic effect. Identifying the effect of hypoxic preconditioning is helpful for better application. However, the influence of hypoxia on dental tissue derived MSCs cultured in 3D was lacking. Thus, this present study is to investigate the gene expression and proteome of dental pulp stem cells(DPSCs) after hypoxic preconditioning...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29316874/liposomal-delivery-of-demineralised-dentine-matrix-for-dental-tissue-regeneration
#3
Genevieve Elizabeth Melling, John Colombo, Steven J Avery, Wayne Ayre, Samuel L Evans, Rachel Jane Waddington, Alastair James Sloan
Current dental restorations have short longevity, consequently there is a need for novel tissue engineering strategies that aim to regenerate the dentine-pulp complex. Dentine matrix contains a myriad of bioactive growth factors and extracellular matrix proteins associated with the recruitment, proliferation and differentiation of dental pulp progenitor cells. Here, we show that demineralised dentine matrix (DDM), from non-carious dentine, can be encapsulated into liposomes for delivery to dental tissue to promote regeneration...
January 9, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29313241/cd146-positive-human-dental-pulp-stem-cells-promote-regeneration-of-dentin-pulp-like-structures
#4
Mikiko Matsui, Tomoko Kobayashi, Takeo W Tsutsui
CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146-) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146- cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146- cells (CD146+/-)...
January 8, 2018: Human Cell
https://www.readbyqxmd.com/read/29306537/metformin-enhances-the-differentiation-of-dental-pulp-cells-into-odontoblasts-by-activating-ampk-signaling
#5
Wei Qin, Xianling Gao, Tao Ma, Michael D Weir, Jing Zou, Bing Song, Zhengmei Lin, Abraham Schneider, Hockin H K Xu
INTRODUCTION: Metformin is a first-line drug for treating type 2 diabetes that regulates the differentiation of mesenchymal stem cells. Its effects on human dental pulp cells (DPCs) remain unknown. This study aimed to investigate the effects of metformin on the proliferation and differentiation of DPCs. METHODS: A live/dead viability assay kit was used to examine the effects of metformin on the cell viability of DPCs. Cell proliferation was analyzed using a cell counting kit (CCK-8; Dojindo, Tokyo, Japan)...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29306073/mir-143-suppresses-the-osteogenic-differentiation-of-dental-pulp-stem-cells-by-inactivation-of-nf-%C3%AE%C2%BAb-signaling-pathway-via-targeting-tnf-%C3%AE
#6
Peng Zhang, Wenli Yang, Guofang Wang, Yajing Li
BACKGROUND: Dental pulp stem cells (DPSCs) are multipotent and play an important role in repairing damaged and/or defective dentinogenesis/osteogenesis. Recent studies have documented the implication of miR-143 in osteogenic differentiation of DPSCs. Nevertheless, the detailed mechanisms of miR-143 involved in the osteogenic differentiation of DPSCs remain to be further elaborated. METHODS: Isolated DPSCs were incubated with osteogenic differentiation medium to induce osteogenic differentiation...
December 30, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29298406/a-miniature-swine-model-for-stem-cell-based-de-novo-regeneration-of-dental-pulp-and-dentin-like-tissue
#7
Xiaofei Zhu, Jie Liu, Zongdong Yu, Chao-An Chen, Hacer Aksel, Adham A Azim, George T-J Huang
The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls...
January 3, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29260758/-regeneration-of-dental-pulp-tissue-using-pulpal-autologous-mesenchymal-stem-cells-and-platelet-rich-plasma
#8
A A Kulakov, D V Goldshtein, E K Krechina, T B Bukharova, A V Volkov, A K Gadzhiev
Regeneration of pulp and dentin could be important in operative dentistry as a method to save teeth. Currently cell population from dental pulp of deciduous and permanent teeth of humans and laboratory animals are isolated and characterized. The paper presents a study on pulp regeneration using autologous mesenchymal stromal cells from pulp of molars in combination with fibrin clot, transplanted in pulp chamber of miniature pigs after pulp removal. The results proved that transplantation of autologous multipotent stromal cells of dental pulp in combination with autologous platelet-rich plasma in pulp chamber of miniature pigs after pulp removal leads to pulp restoration and reparative dentinogenesis with dentinal bridge formation on the 30th day...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29260756/-optimization-of-reparative-dentinogenesis-in-experimental-osteoporosis
#9
N I Bykova, S V Sirak, T L Kobylkina, A V Odolsky, I M Bykov, A V Arutyunov
The aim of the study was to assess histochemical changes of the dental pulp in direct pulp capping/experimental osteoporosis animal model. The study was performed on 20 two-year sheep with simulated acute pulpitis divided in 2 groups: main (15 animals/120 teeth) and control (5 animals/40 teeth). Direct pulp capping in the main group included tissue-engineered structure composed of a hydrogel PuraMatrix/3DM with ectomesenchymal stem cells immobilized on collagen sponge. In the control group collagen sponges with hydrocortisone furatsilin, chondroitin sulfate, аnaesthesinum were used for the same purpose...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/29259707/isolation-of-dental-pulp-stem-cells-with-high-osteogenic-potential
#10
REVIEW
Takazumi Yasui, Yo Mabuchi, Satoru Morikawa, Katsuhiro Onizawa, Chihiro Akazawa, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki
Dental pulp stem cells/progenitor cells (DPSCs) can be easily obtained and can have excellent proliferative and mineralization potentials. Therefore, many studies have investigated the isolation and bone formation of DPSCs. In most previous reports, human DPSCs were traditionally isolated by exploiting their ability to adhere to plastic tissue culture dishes. DPSCs isolated by plastic adherence are frequently contaminated by other cells, which limits the ability to investigate their basic biology and regenerative properties...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29258387/microfluidic-encapsulation-supports-stem-cell-viability-proliferation-and-neuronal-differentiation
#11
Lorena Hidalgo San Jose, Phil Stephens, Bing Song, David Barrow
Stem cell encapsulation technology demonstrates much promise for the replacement of damaged tissue in several diseases, including spinal cord injury (SCI). The use of biocompatible microcapsules permits the control of stem cell fate in situ to facilitate the replacement of damaged/lost tissue. In this work, a novel customized microfluidic device was developed for the reproducible encapsulation of Neural Stem Cells (NSCs) and Dental Pulp Stem Cells (DPSCs) within monodisperse, alginate-collagen microcapsules...
December 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29240205/regenerative-treatment-of-periodontal-intrabony-defects-using-autologous-dental-pulp-stem-cells-a-1-year-follow-up-case-series
#12
Mario Aimetti, Francesco Ferrarotti, Mara Noemi Gamba, Marta Giraudi, Federica Romano
The present case series aimed to explore the potential clinical benefits of the application of dental pulp stem cells (DPSCs) in the regenerative treatment of deep intrabony defects. A total of 11 isolated intrabony defects in 11 chronic periodontitis patients were accessed with a minimally invasive flap and filled with DPSCs loaded on a collagen sponge. A tooth requiring extraction for impaction or malpositioning was used as an autologous source for DPSCs. An average clinical attachment level gain of 4.7 ± 1...
January 2018: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/29237486/transplantation-of-dental-pulp-stem-cells-improves-long-term-diabetic-polyneuropathy-together-with-improvement-of-nerve-morphometrical-evaluation
#13
Maiko Omi, Masaki Hata, Nobuhisa Nakamura, Megumi Miyabe, Shogo Ozawa, Hitoshi Nukada, Masami Tsukamoto, Kazunori Sango, Tatsuhito Himeno, Hideki Kamiya, Jiro Nakamura, Jun Takebe, Tatsuaki Matsubara, Keiko Naruse
BACKGROUND: Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, the effects of cell transplantation on long-term diabetic polyneuropathy have not been investigated. In this study, we investigated whether the transplantation of dental pulp stem cells (DPSCs) ameliorated long-term diabetic polyneuropathy in streptozotocin (STZ)-induced diabetic rats. METHODS: Forty-eight weeks after STZ injection, we transplanted DPSCs into the unilateral hindlimb skeletal muscles...
December 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29227501/chondrogenic-potential-of-macroporous-biodegradable-cryogels-based-on-synthetic-poly-%C3%AE-amino-acids
#14
T Sedlačík, O K Acar, H Studenovská, I Kotelnikov, J Kučka, Z Konečná, T Zikmund, J Kaiser, G T Kose, F Rypáček
In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-l-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-l-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers...
December 11, 2017: Soft Matter
https://www.readbyqxmd.com/read/29215832/-activation-of-regeneration-of-red-and-white-pulp-of-the-spleen-after-the-combined-transplantation-of-hsc-and-mscs-in-terms-of-exposure-to-ionizing-radiation
#15
I Yu Maklakova, D Yu Grebnev, A P Yastrebov
The purpose of this work was to study the effect of combined transplantation of multipotent mesenchymal stromal (MSCS) and hematopoietic stem cells (HSCs) isolated from the placenta, on the regeneration of white and red pulp of the spleen under physiological conditions and in conditions of exposure to ionizing radiation. Methods. The experiments were performed with laboratory mice-males. We studied the influence of ionizing radiation dose of 4.0 Gy. Animals of the experimental group were intravenously infused into MMSC and GSK respectively at a dose of 6 million cells/kg and 330 thousand cells/kg, suspended in 0...
April 2017: Patologicheskaia Fiziologiia i èksperimental'naia Terapiia
https://www.readbyqxmd.com/read/29215170/synthetic-light-curable-polymeric-materials-provide-a-supportive-niche-for-dental-pulp-stem-cells
#16
Kyle H Vining, Jacob C Scherba, Alaina M Bever, Morgan R Alexander, Adam D Celiz, David J Mooney
Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used...
December 7, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29201060/osteogenic-differentiation-of-hdpscs-on-biogenic-bone-apatite-thin-films
#17
Michele Bianchi, Alessandra Pisciotta, Laura Bertoni, Matteo Berni, Alessandro Gambardella, Andrea Visani, Alessandro Russo, Anto de Pol, Gianluca Carnevale
A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29201058/comparing-circadian-dynamics-in-primary-derived-stem-cells-from-different-sources-of-human-adult-tissue
#18
Eve H Rogers, Sandra A Fawcett, Vanja Pekovic-Vaughan, John A Hunt
Optimising cell/tissue constructs so that they can be successfully accepted and integrated within a host body is essential in modern tissue engineering. To do this, adult stem cells are frequently utilised, but there are many aspects of their environment in vivo that are not completely understood. There is evidence to suggest that circadian rhythms and daily circadian temporal cues have substantial effects on stem cell activation, cell cycle, and differentiation. It was hypothesised that the circadian rhythm in human adult stem cells differs depending on the source of tissue and that different entraining signals exert differential effects depending on the anatomical source...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29200956/stem-cells-from-cryopreserved-human-dental-pulp-tissues-sequentially-differentiate-into-definitive-endoderm-and-hepatocyte-like-cells-in-vitro
#19
Young-Jin Han, Young-Hoon Kang, Sarath Belame Shivakumar, Dinesh Bharti, Young-Bum Son, Yong-Ho Choi, Won-Uk Park, June-Ho Byun, Gyu-Jin Rho, Bong-Wook Park
We previously described a novel tissue cryopreservation protocol to enable the safe preservation of various autologous stem cell sources. The present study characterized the stem cells derived from long-term cryopreserved dental pulp tissues (hDPSCs-cryo) and analyzed their differentiation into definitive endoderm (DE) and hepatocyte-like cells (HLCs) in vitro. Human dental pulp tissues from extracted wisdom teeth were cryopreserved as per a slow freezing tissue cryopreservation protocol for at least a year...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29199334/-effect-of-transfected-ephb4-on-osteogenic-differentiation-in-stem-cells-from-human-exfoliated-deciduous-teeth
#20
Xiao-Ming Li, Chang-Yong Yuan, Shao-Yue Zhu, Zong-Xiang Liu, Peng-Lai Wang
PURPOSE: To evaluate the effect of stem cells from human exfoliated deciduous teeth (SHED) transfected with EphB4 gene in regulating osteogenic differentiation. METHODS: Human dental pulp tissue were harvested from extracted deciduous teeth and digested by collagenase and dispase. The SHEDs were transfected with transgenic (hEphB4-GFP) vector or empty vector (GFP-vector). Real time-polymerase chain reaction(real time-PCR) analysis and Western blot were used to detect the expression of EphB4 in SHEDs after transfection...
August 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
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