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Pulp stem cells

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https://www.readbyqxmd.com/read/28427322/dental-pulp-pluripotent-like-stem-cells-dppsc-a-new-stem-cell-population-with-chromosomal-stability-and-osteogenic-capacity-for-biomaterials-evaluation
#1
Raquel Núñez-Toldrà, Ester Martínez-Sarrà, Carlos Gil-Recio, Miguel Ángel Carrasco, Ashraf Al Madhoun, Sheyla Montori, Maher Atari
BACKGROUND: Biomaterials are widely used to regenerate or substitute bone tissue. In order to evaluate their potential use for clinical applications, these need to be tested and evaluated in vitro with cell culture models. Frequently, immortalized osteoblastic cell lines are used in these studies. However, their uncontrolled proliferation rate, phenotypic changes or aberrations in mitotic processes limits their use in long-term investigations. Recently, we described a new pluripotent-like subpopulation of dental pulp stem cells derived from the third molars (DPPSC) that shows genetic stability and shares some pluripotent characteristics with embryonic stem cells...
April 21, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28416308/implantation-of-endothelial-cells-with-mesenchymal-stem-cells-accelerates-dental-pulp-tissue-regeneration-healing-in-pulpotomized-rat-molars
#2
Yukiko Sueyama, Tomoatsu Kaneko, Takafumi Ito, Reika Kaneko, Takashi Okiji
INTRODUCTION: This study aimed to examine whether the implantation of mesenchymal stem cells (MSCs) with endothelial cells (ECs) accelerates pulp tissue regeneration/healing and induces dentin bridge formation in a rat model of molar coronal pulp regeneration. METHODS: The maxillary first molars of Wistar rats were subjected to pulpotomy. Then, pulp chambers were implanted with biodegradable hydrogel-made scaffolds carrying MSCs together or without dermal microvascular ECs, and the cavities were sealed with mineral trioxide aggregate...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28412509/a-monoclonal-antibody-recognizes-undifferentiation-specific-carbohydrate-moieties-expressed-on-cell-surface-of-the-human-dental-pulp-cells
#3
Kyung-Jung Kang, Seon-Yle Ko, Chun-Jeih Ryu, Young-Joo Jang
Human dental pulp cells are obtained from dental pulp tissue, and have the ability to form dentin and a pulp-like complex. Although adult stem cells have been identified from the primary culture by using specific cell surface markers, the identity of surface markers for the purification of stem cells within the dental pulp population are still unclear. Previously, we had constructed monoclonal antibodies against the undifferentiated cell-specific surface markers of human dental pulp cells (hDPCs) by performing decoy immunization...
April 6, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28410532/role-of-p16-ink4a-and-bmi-1-in-oxidative-stress-induced-premature-senescence-in-human-dental-pulp-stem-cells
#4
Cristina Mas-Bargues, José Viña-Almunia, Marta Inglés, Jorge Sanz-Ros, Juan Gambini, José Santiago Ibáñez-Cabellos, José Luis García-Giménez, José Viña, Consuelo Borrás
Human dental pulp stem cells (hDPSCs) are a source for cell therapy. Before implantation, an in vitro expansion step is necessary, with the inconvenience that hDPSCs undergo senescence following a certain number of passages, loosing their stemness properties. Long-term in vitro culture of hDPSCs at 21% (ambient oxygen tension) compared with 3-6% oxygen tension (physiological oxygen tension) caused an oxidative stress-related premature senescence, as evidenced by increased β-galactosidase activity and increased lysil oxidase expression, which is mediated by p16(INK4a) pathway...
April 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/28410232/vacquinol-1-inducible-cell-death-in-glioblastoma-multiforme-is-counter-regulated-by-trpm7-activity-induced-by-exogenous-atp
#5
Philip Sander, Haouraa Mostafa, Ayman Soboh, Julian M Schneider, Andrej Pala, Ann-Kathrin Baron, Barbara Moepps, C Rainer Wirtz, Michael Georgieff, E Marion Schneider
Glioblastomas (GBM) are the most malignant brain tumors in humans and have a very poor prognosis. New therapeutic options are urgently needed. A novel drug, Vacquinol-1 (Vac), a quinolone derivative, displays promising properties by inducing rapid cell death in GBM but not in non-transformed tissues. Features of this type of cell death are compatible with a process termed methuosis. Here we tested Vac on a highly malignant glioma cell line observed by long-term video microscopy. Human dental-pulp stem cells (DPSCs) served as controls...
March 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28394043/efficacy-of-stem-cells-on-periodontal-regeneration-systematic-review-of-pre-clinical-studies
#6
REVIEW
S A Tassi, N Z Sergio, M Y O Misawa, C C Villar
This systematic review aims to evaluate mesenchymal stem cells (MSC) periodontal regenerative potential in animal models. MEDLINE, EMBASE and LILACS databases were searched for quantitative pre-clinical controlled animal model studies that evaluated the effect of local administration of MSC on periodontal regeneration. The systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement guidelines. Twenty-two studies met the inclusion criteria. Periodontal defects were surgically created in all studies...
April 10, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28386853/assessment-of-bone-regeneration-of-a-tissue-engineered-bone-complex-using-human-dental-pulp-stem-cells-poly-%C3%AE%C2%B5-caprolactone-biphasic-calcium-phosphate-scaffold-constructs-in-rabbit-calvarial-defects
#7
Natkrita Wongsupa, Thongchai Nuntanaranont, Suttatip Kamolmattayakul, Nuttawut Thuaksuban
The objective of the present study was to investigate the effect of a fabricated combination of poly-ɛ-caprolactone (PCL)-biphasic calcium phosphate (BCP) with the modified melt stretching and multilayer deposition (mMSMD) technique on human dental pulp stem cell (hDPSC) differentiation to be osteogenic like cells for bone regeneration of calvarial defects in rabbit models. hDPSCs extracted from human third molars were seeded onto mMSMD PCL-BCP scaffolds and the osteogenic gene expression was tested prior to implantation in vivo...
May 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28380697/graphene-oxide-enrichment-collagen-membranes-improves-dpscs-differentiation-and-controls-inflammation-occurrence
#8
Milena Radunovic, Marianna De Colli, Patrizia De Marco, Chiara Di Nisio, Antonella Fontana, Adriano Piattelli, Amelia Cataldi, Susi Zara
Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28377925/isolation-of-mesenchymal-stem-cells-from-human-deciduous-teeth-pulp
#9
Aileen I Tsai, Hsiang-Hsi Hong, Wey-Ran Lin, Jen-Fen Fu, Chih-Chun Chang, I-Kuan Wang, Wen-Hung Huang, Cheng-Hao Weng, Ching-Wei Hsu, Tzung-Hai Yen
This study aimed to identify predictors of success rate of mesenchymal stem cell (MSC) isolation from human deciduous teeth pulp. A total of 161 deciduous teeth were extracted at the dental clinic of Chang Gung Memorial Hospital. The MSCs were isolated from dental pulps using a standard protocol. In total, 128 colonies of MSCs were obtained and the success rate was 79.5%. Compared to teeth not yielding MSCs successfully, those successfully yielding MSCs were found to have less severe dental caries (no/mild-to-moderate/severe: 63...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28369799/effect-of-dental-pulp-stem-cells-in-mptp-induced-old-aged-mice-model
#10
Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Christopher Simon, Quan Fu Gan, Vui King Vincent-Chong, Vasudevan Mani, Kesavanarayanan Krishnan Selvarajan, Vellayan Subramaniam, Sabri Musa, Noor Hayaty Abu Kasim
Parkinson's disease (PD) is a neurodegenerative disease caused by the loss of dopaminergic (DA-ergic) neurons in the substantia nigra (SN) and represent as a huge threat to the geriatric population. Cell-replacement therapies (CRTs) have been proposed as a promising strategy to slow down or replace neuronal loss. Among the widely available cell sources, dental pulp stem cells (DPSCs) portray as an attractive source primarily due to their neural crest origin, ease of tissue procurement and less ethical hurdles MATERIALS AND METHODS: We first demonstrated the in vitro differentiation ability of DPSCs towards DA-ergic-like cells before evaluating their neuro-protection/neuro-restoration capacities in MPTP-induced mice...
March 30, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28368079/the-effect-of-extracellular-acidosis-on-the-behaviour-of-mesenchymal-stem-cells-in-vitro
#11
A Massa, F Perut, T Chano, A Woloszyk, T A Mitsiadis, S Avnet, N Baldini
The stem cell fraction of a cell population is finely tuned by stimuli from the external microenvironment. Among these stimuli, a decrease of extracellular pH (pHe) may occur in a variety of physiological and pathological conditions, including hypoxia and inflammation. In this study, by using bone marrow stem cells and dental pulp stem cells, we provided evidence that extracellular acidosis endows the maintenance of stemness in mesenchymal cells. Indeed, continuous exposure for 21 d to low pHe (6.5-6.8) conditions impaired the osteogenic differentiation of both cell types...
April 3, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28366235/zinc-bioglasses-regulate-mineralization-in-human-dental-pulp-stem-cells
#12
Mei Huang, Robert G Hill, Simon C F Rawlinson
OBJECTIVE: A promising strategy in regenerative endodontics is the combination of human dental pulp stem cells (hDPSCs) with an appropriate biomaterial substrate. The effects of zinc and zinc containing bioactive glasses (ZnBGs) on hDPSCs have been characterized in this study. METHODS: ZnBGs were designed and produced. Then the odontogenic differentiation and mineralization potential of hDPSCs upon ZnBGs treatment were investigated. RESULTS: Free Zn ions (0-5ppm) enhanced proliferation and alkaline phosphatase (ALP) activity of hDPSCs...
March 30, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28365680/pro-inflammatory-cytokine-tnf-%C3%AE-attenuates-bmp9-induced-osteo-odontoblastic-differentiation-of-the-stem-cells-of-dental-apical-papilla-scaps
#13
Feilong Wang, Ying Jiang, Xia Huang, Qiao Liu, Yan Zhang, Wenping Luo, Fugui Zhang, Pengfei Zhou, Juhong Lin, Hongmei Zhang
BACKGROUND/AIMS: Periapical periodontitis is a common oral disease caused by bacterial invasion of the tooth pulp, which usually leads to local release of pro-inflammatory cytokines and osteolytic lesion. This study is intended to examine the effect of TNF-α on BMP9-induced osteogenic differentiation of the stem cells of dental apical papilla (SCAPs). METHODS: Rat model of periapical periodontitis was established. TNF-α expression was assessed. Osteogenic markers and ectopic bone formation in iSCAPs were analyzed upon BMP9 and TNF-α treatment...
March 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28364342/in-vitro-proliferation-and-osteogenic-differentiation-of-endometrial-stem-cells-and-dental-pulp-stem-cells
#14
Fahimeh Sadat Tabatabaei, Maryam Torshabi
Stem-cell-based therapies were introduced aiming to overcome the limitations of the existing procedures for regeneration of mineralized tissues. Stem cells isolated from the endometrial tissue and dental pulp have the capacity to differentiate into various functional cells including osteoblasts. However, studies comparing their ability to regenerate mineralized tissue are lacking. The purpose of this study was to compare the proliferation and osteogenic differentiation potential of endometrial stem cells (EnSCs) and dental pulp stem cells (DPSCs) using in vitro cell culture technique...
March 31, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28343926/cobalt-chloride-enhances-the-stemness-of-human-dental-pulp-cells
#15
Kantaporn Laksana, Sireerat Sooampon, Prasit Pavasant, Wannakorn Sriarj
INTRODUCTION: Hypoxia is a factor in controlling stem cell stemness. We investigated if cobalt chloride (CoCl2), a chemical agent that mimics hypoxia in vitro, affected human dental pulp cell (hDPC) stemness by examining cell proliferation, stem cell marker expression, and osteogenic differentiation. METHODS: hDPCs were cultured with or without 25 or 50 μmol/L CoCl2. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to determine cell proliferation...
March 23, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28342024/towards-optimization-of-odonto-osteogenic-bioengineering-in-vitro-comparison-of-simvastatin-sodium-fluoride-melanocyte-stimulating-hormone
#16
Vahid Zijah, Roya Salehi, Marziyeh Aghazadeh, Mohammad Samiei, Effat Alizadeh, Soodabeh Davaran
Tissue engineering has emerged as a potential therapeutic option for dental problems in recent years. One of the policies in tissue engineering is to use both scaffolds and additive factors for enhancing cell responses. This study aims to evaluate and compare the effect of three types of biofactors on poly-caprolactone-poly-ethylene glycol-poly caprolactone (PCL-PEG-PCL) nanofibrous scaffold on human dental pulp stem cell (hDPSCs) engineering. The PCL-PEG-PCL copolymer was synthesized with ring opening polymerization method, and its nanofiber scaffold was prepared by electrospinning method...
March 25, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28328017/dental-pulp-stem-cells-promote-regeneration-of-damaged-neuron-cells-on-the-cellular-model-of-alzheimer-s-disease
#17
Feixiang Wang, Yali Jia, Jiajing Liu, Jinglei Zhai, Ning Cao, Wen Yue, Huixia He, Xuetao Pei
Alzheimer's disease (AD) is an incurable neurodegenerative disease and many types of stem cells have been used in AD therapy with some favorable effects. In this study, we investigated the potential therapeutical effects of human dental pulp stem cells (hDPSCs) on AD cellular model which established by okadaic acid (OA)-induced damage to human neuroblastoma cell line, SH-SY5Y, in vitro for 24h. After confirmed the AD cellular model, the cells were co-culture with hDPSCs by transwell co-culture system till 24 h for treatment...
March 22, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28314378/novel-cryopreservation-method-for-the-effective-collection-of-dental-pulp-stem-cells
#18
Yusuke Takebe, Seiko Tatehara, Tatsuhiro Fukushima, Reiko Tokuyama-Toda, Rika Yasuhara, Kenji Mishima, Kazuhito Satomura
Dental pulp stem cells (DPSCs) are an attractive cell source for use in cell-based therapy, regenerative medicine, and tissue engineering because DPSCs have high cell proliferation ability and multi-differentiation capacity. However, several problems are associated with the collection and preservation of DPSCs for future cell-based therapy. In addition, the isolation of DPSCs for cryopreservation is time-consuming and expensive. In this study, we developed a novel cryopreservation method for dental pulp tissues that can isolate suitable DPSCs after thawing cryopreserved tissue...
March 17, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28298928/biological-characteristics-of-fluorescent-superparamagnetic-iron-oxide-labeled-human-dental-pulp-stem-cells
#19
Liang Ma, Ming-Wei Li, Yu Bai, Hui-Hui Guo, Sheng-Chao Wang, Qing Yu
Tracking transplanted stem cells is necessary to clarify cellular properties and improve transplantation success. In this study, we investigate the effects of fluorescent superparamagnetic iron oxide particles (SPIO) (Molday ION Rhodamine-B™, MIRB) on biological properties of human dental pulp stem cells (hDPSCs) and monitor hDPSCs in vitro and in vivo using magnetic resonance imaging (MRI). Morphological analysis showed that intracellular MIRB particles were distributed in the cytoplasm surrounding the nuclei of hDPSCs...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28287912/osteogenic-differentiation-of-mesenchymal-stromal-cells-a-comparative-analysis-between-human-subcutaneous-adipose-tissue-and-dental-pulp
#20
Iolanda D'Alimonte, Filiberto Mastrangelo, Patricia Giuliani, Laura Pierdomenico, Marco Marchisio, Mariachiara Zuccarini, Patrizia Di Iorio, Raimondo Quaresima, Francesco Caciagli, Renata Ciccarelli
White adipose tissue is a source of mesenchymal stromal/stem cells (MSCs) that are actively studied for their possible therapeutic use in bone tissue repair/remodeling. To better appreciate the osteogenic potential of these cells, we compared some properties of MSCs from human subcutaneous adipose tissue [subcutaneous-adipose stromal cells (S-ASCs)] and dental pulp stem cell (DPSCs) of third-impacted molars, the latter representing a well-established MSC source. Both undifferentiated cell types showed similar fibroblast-like morphology and mesenchymal marker expression...
March 13, 2017: Stem Cells and Development
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