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Pulp stem cells

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https://www.readbyqxmd.com/read/29770705/gingival-mesenchymal-stem-cells-outperform-haploidentical-dental-pulp-derived-mesenchymal-stem-cells-in-proliferation-rate-migration-ability-and-angiogenic-potential
#1
Ioannis Angelopoulos, Claudia Brizuela, Maroun Khoury
High donor variation makes comparison studies between different dental sources dubious. Dental tissues offer a rare opportunity for comparing the biological characteristics of haploidentical mesenchymal stem cells (MSCs) isolated from the same donor. The objective was to identify the optimal dental source of MSCs through a biological and functional comparison of haploidentical MSCs from gingival (GMSCs) and dental pulp stem cells (DPSCs) focusing mainly on their angiogenic potential. The comparison study included (1) surface markers expression, (2) mesodermal differentiation capacity (chondrogenic, adipogenic, and osteogenic), (3) proliferation, (4) migration potential, (5) ability to form colony units, and (6) angiogenic potential in vitro and in vivo...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29770323/dental-mesenchymal-stem-cell-based-translational-regenerative-dentistry-from-artificial-to-biological-replacement
#2
REVIEW
Mona K Marei, Rania M El Backly
Dentistry is a continuously changing field that has witnessed much advancement in the past century. Prosthodontics is that branch of dentistry that deals with replacing missing teeth using either fixed or removable appliances in an attempt to simulate natural tooth function. Although such "replacement therapies" appear to be easy and economic they fall short of ever coming close to their natural counterparts. Complications that arise often lead to failures and frequent repairs of such devices which seldom allow true physiological function of dental and oral-maxillofacial tissues...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29765407/effects-of-transplanted-heparin-poloxamer-hydrogel-combining-dental-pulp-stem-cells-and-bfgf-on-spinal-cord-injury-repair
#3
Lihua Luo, Abdullkhaleg Ali Albashari, Xiaoyan Wang, Ling Jin, Yanni Zhang, Lina Zheng, Jianjian Xia, Helin Xu, Yingzheng Zhao, Jian Xiao, Yan He, Qingsong Ye
Spinal cord injury (SCI) is one of serious traumatic diseases of the central nervous system and has no effective treatment because of its complicated pathophysiology. Tissue engineering strategy which contains scaffolds, cells, and growth factors can provide a promising treatment for SCI. Hydrogel that has 3D network structure and biomimetic microenvironment can support cellular growth and embed biological macromolecules for sustaining release. Dental pulp stem cells (DPSCs), derived from cranial neural crest, possess mesenchymal stem cell (MSC) characteristics and have an ability to provide neuroprotective and neurotrophic properties for SCI treatment...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29764492/mineral-trioxide-aggregate-affects-cell-viability-and-induces-apoptosis-of-stem-cells-from-human-exfoliated-deciduous-teeth
#4
Chia-Ling Tsai, Mu-Chan Ke, Yi-Hao Chen, Hsi-Kung Kuo, Hun-Ju Yu, Chueh-Tan Chen, Ya-Chi Tseng, Pei-Chin Chuang, Pei-Chang Wu
BACKGROUND: Mineral trioxide aggregate (MTA) is widely used for pulp-capping procedures in permanent teeth and as a gold standard material in endodontics. The aim of the study was to investigate the effect of MTA on cell viability and apoptosis when MTA is directly in contact with Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs). METHODS: MTA was mixed and coated in the bottom of a 24-well plate. SHEDs collected and cultured from normal exfoliated human deciduous teeth (passages 3-4) were seeded on square cover glasses...
May 15, 2018: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/29763345/tissue-engineering-of-necrotic-dental-pulp-of-immature-teeth-with-apical-periodontitis-in-dogs-radiographic-and-histological-evaluation
#5
Eman A El Ashiry, Najlaa M Alamoudi, Mahmoud K El Ashiry, Hagar A Bastawy, Douaa A El Derwi, Hazem M Atta
AIM: To evaluate tissue engineering technology to regenerate pulp-dentin like tissues in pulp canals of immature necrotic permanent teeth with apical periodontitis in dogs. STUDY DESIGN: The study was performed on 36 teeth in 12 dogs. The experiment was carried out using split mouth design. In each dog 3 teeth were selected for implementing the study procedure. Apical periodontitis was induced in Group A and B teeth. Group (A): immature upper left 2nd permanent incisors that were transplanted with a construct of autologous dental pulp stem cells with growth factors seeded in a chitosn hydrogel scaffold...
May 15, 2018: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/29760738/dental-mesenchymal-stem-stromal-cells-and-their-exosomes
#6
REVIEW
Peter Stanko, Ursula Altanerova, Jana Jakubechova, Vanda Repiska, Cestmir Altaner
Stem cells derived from human dental pulp tissue (DP-MSC) differ from the other mesenchymal stem cells prepared from bone marrow or adipose tissue due to their embryonic origin from the neural crest and are of special interest because of their neurotropic character. Furthermore, the therapeutic potential of DP-MSCs is realized through paracrine action of extracellularly released components, for which exosomes play an important role. In this review, we intend to explore the properties of these cells with an emphasis on exosomes...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29752081/effect-of-polyhydroxybutyrate-chitosan-bioglass-nanofiber-scaffold-on-proliferation-and-differentiation-of-stem-cells-from-human-exfoliated-deciduous-teeth-into-odontoblast-like-cells
#7
Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Batool Hashemibeni, Abbas Ali Khademi
Scaffolds and their characteristics play a central role in tissue engineering. The purpose of this study was to determine the effects of Polyhydroxybutyrate (PHB)/Chitosan/nano-bioglass (nBG) nanofiber scaffold made using the electrospinning method, on the proliferation and differentiation of stem cells obtained from human exfoliated deciduous teeth into odontoblast-like cells. In this experimental study, the pulps of the molten deciduous teeth were isolated, thereafter, the stem cells from human exfoliated deciduous teeth (SHED) were extracted and then the 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability percentage...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29744863/photobiomodulation-therapy-and-vitamin-c-on-longevity-of-cell-sheets-of-human-dental-pulp-stem-cells
#8
Ana C F Pedroni, Ivana M A Diniz, Gabriela L Abe, Maria S Moreira, Carla R Sipert, Márcia M Marques
Photobiomodulation therapy (PBMT) can improve processes relevant to tissue regeneration, such as survival, proliferation, migration, and differentiation of cells, including stem cells. Thus, PBMT could be applied as auxiliary therapy for tissue regeneration. Cell sheets (CSs) induced by vitamin C (VC) can generate large amount of cells, which would also be useful for tissue regeneration. VC and PBMT cause opposite effects on cell metabolism (e.g., VC is antioxidative, and PBMT generates reactive oxygen species); however, hDPSC CSs were formed when VC and PBMT+VC were applied...
May 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29741358/fibrous-topography-potentiated-canonical-wnt-signaling-directs-the-odontoblastic-differentiation-of-dental-pulp-derived-stem-cells
#9
Saeed Ur Rahman, Joung-Hwan Oh, Young-Dan Cho, Shin Hye Chung, Gene Lee, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, the mechanisms by which the nanofibrous matrix promotes mesenchymal stem cell (MSC) differentiation are largely unknown. Here, we investigated the mechanisms which underlie nanofibrous matrix-induced odontoblastic differentiation of human dental pulp MSCs (DP-MSCs). An electrospun polystyrene nanofibrous (PSF) matrix was prepared, and the cell responses to the PSF matrix were assessed in comparison with those on conventional tissue culture dishes...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29737458/dental-pulp-stem-cells-exploration-in-a-novel-animal-model-the-tasmanian-devil-sarcophilus-harrisii
#10
REVIEW
Chelsea M Graham, Karlea L Kremer, Simon A Koblar, Monica A Hamilton-Bruce, Stephen B Pyecroft
Dental pulp stem cells (DPSC) are a heterogeneous population of highly proliferative stem cells located in the soft inner pulp tissue of the tooth. Demonstrated to have an affinity for neural differentiation, DPSC have been reported to generate functional Schwann cells (SC) through in vitro differentiation. Both DPSC and SC have neural crest origins, recently a significant population of DPSC have been reported to derive from peripheral nerve-associated glia. The predisposition DPSC have towards the SC lineage is not only a very useful tool for neural regenerative therapies in the medical field, it also holds great promise in the veterinary field...
May 8, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29731830/rho-rho-associated-protein-kinase-signaling-pathway-mediated-downregulation-of-runt-related-transcription-factor-2-expression-promotes-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts
#11
Xiaoqing Huang, Xiaoling Chen, Hongbai Chen, Dongwei Xu, Chen Lin, Bin Peng
The present study investigated the role of runt-related transcription factor 2 (Runx2) in regulating the differentiation of human dental pulp stem cells (hDPSCs) into odontoblasts under the mediation of the Rho/Rho-associated protein kinase (ROCK) signaling pathway. hDPSCs and human bone marrow mesenchymal stem cells (hBMSCs) were mineralized to induce differentiation. The expression levels of odontoblast- and osteoblast-specific proteins, dentin sialophosphoprotein (DSPP), osteocalcin (OCN) and Runx2, were measured using western blot analysis...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29731777/the-hypoxia-mimetic-agent-cobalt-chloride-differently-affects-human-mesenchymal-stem-cells-in-their-chondrogenic-potential
#12
Gabriella Teti, Stefano Focaroli, Viviana Salvatore, Eleonora Mazzotti, Laura Ingra', Antonio Mazzotti, Mirella Falconi
Adult stem cells are a promising cell source for cartilage regeneration. They resided in a special microenvironment known as the stem-cell niche, characterized by the presence of low oxygen concentration. Cobalt chloride (CoCl2 ) imitates hypoxia in vitro by stabilizing hypoxia-inducible factor-alpha (HIF-1 α ), which is the master regulator in the cellular adaptive response to hypoxia. In this study, the influence of CoCl2 on the chondrogenic potential of human MSCs, isolated from dental pulp, umbilical cord, and adipose tissue, was investigated...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29730288/comparative-characterization-of-stem-cells-from-human-exfoliated-deciduous-teeth-dental-pulp-and-bone-marrow-derived-mesenchymal-stem-cells
#13
Ryo Kunimatsu, Kengo Nakajima, Awada Tetsuya, Yuji Tsuka, Takaharu Abe, Kazuyo Ando, Tomoka Hiraki, Aya Kimura, Kotaro Tanimoto
OBJECTIVES: Mesenchymal stem cells (MSCs) are used clinically in tissue engineering and regenerative medicine. The proliferation and osteogenic differentiation potential of MSCs vary according to factors such as tissue source and cell population heterogeneity. Dental tissue has received attention as an easily accessible source of high-quality stem cells. In this study, we compared the in vitro characteristics of dental pulp stem cells from deciduous teeth (SHED), human dental pulp stem cells (hDPSCs), and human bone marrow mesenchymal stem cells (hBMSCs)...
May 3, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29728886/development-of-a-novel-large-animal-model-to-evaluate-human-dental-pulp-stem-cells-for-articular-cartilage-treatment
#14
Tiago Lazzaretti Fernandes, Kazunori Shimomura, Andre Asperti, Carla Cristina Gomes Pinheiro, Heloísa Vasconcellos Amaral Caetano, Claudia Regina G C M Oliveira, Norimasa Nakamura, Arnaldo José Hernandez, Daniela Franco Bueno
PURPOSE: Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. The ability of human Dental Pulp Stem Cells (DPSCs) to differentiate into chondroblasts in vitro suggests that this stem cell type may be useful for tissue bioengineering. However, we have yet to identify a study of large animal models in which DPSCs were used to repair articular cartilage. Therefore, this study aimed to describe a novel treatment for cartilage lesion with DPSCs on a large animal model...
May 4, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29726734/preparation-and-characterization-of-titanium-segmented-polyurethane-composites-for-bone-tissue-engineering
#15
Fernando Javier Aguilar-Perez, Rossana Vargas-Coronado, Jose Manuel Cervantes-Uc, Juan Valerio Cauich-Rodriguez, Raul Rosales-Ibañez, Juan Jose Pavon-Palacio, Yadir Torres-Hernandez, Jose Antonio Rodriguez-Ortiz
Segmented polyurethanes were prepared with polycaprolactone diol as soft segment and 4,4-methylene-bis cyclohexyl diisocyanate and l-glutamine as the rigid segment. These polyurethanes were filled with 1 wt.% to 5 wt.% titanium particles (Ti), physicochemically characterized and their biocompatibility assessed using human dental pulp stem cells and mice osteoblasts. Physicochemical characterization showed that composites retained the properties of the semicrystalline polyurethane as they exhibited a glass transition temperature (Tg ) between -35°C and -45°C, melting temperature (Tm ) at 52°C and crystallinity close to 40% as determined by differential scanning calorimetry...
January 1, 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29725075/dental-pulp-stem-cells-in-regenerative-medicine
#16
P Hollands, D Aboyeji, M Orcharton
The mesenchymal stem cells (dental pulp stem cells; DPSC) found inside teeth represent a significant future source of stem cells for regenerative medicine procedures. This review describes the ontogeny of DPSC; the laboratory processing and collection of DPSC; the immuno-cytochemical characterisation of DPSC; the differentiation between adult DPSC and DPSC obtained from exfoliated deciduous teeth (SHED) and their potential use in regenerative medicine procedures in the future both in dental and general medical applications...
May 4, 2018: British Dental Journal
https://www.readbyqxmd.com/read/29724156/pulp-regeneration-concepts-for-non-vital-teeth-from-tissue-engineering-to-clinical-approaches
#17
Valérie Orti, Pierre-Yves Collart-Dutilleul, Sofía Silvia Piglionico, Orsolya Pall, Frédéric Cuisinier, Ivan Vladislavov Panayotov
Following the basis of tissue engineering (Cells - Scaffold - Bioactive molecules), regenerative endodontic has emerged as a new concept of dental treatment. Clinical procedures have been proposed by endodontic practitioners willing to promote regenerative therapy. Preserving pulp vitality was a first approach. Later procedures aimed to regenerate a vascularized pulp in necrotic root canals. However, there is still no protocol allowing an effective regeneration of necrotic pulp tissue either in immature or mature teeth...
May 4, 2018: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/29707538/insulin-like-growth-factor-axis-expression-in-dental-pulp-cells-derived-from-carious-teeth
#18
Hanaa Esa Alkharobi, Hasanain Al-Khafaji, James Beattie, Deirdre Ann Devine, Reem El-Gendy
The insulin-like growth factor (IGF) axis plays an important role in dental tissue regeneration and most components of this axis are expressed in human dental pulp cells (DPCs). In our previous study, we analyzed IGF axis gene expression in DPCs and demonstrated a novel role of IGF binding protein (IGFBP)-2 and -3 in coordinating mineralized matrix formation in differentiating DPCs. A more recent study from our laboratory partially characterized dental pulp stem cells from teeth with superficial caries (cDPCs) and showed that their potential to differentiate odontoblasts and/or into osteoblasts is enhanced by exposure to the mild inflammatory conditions characteristic of superficial caries...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29707181/dental-stem-cells-harvested-from-third-molars-combined-with-bioactive-glass-can-induce-signs-of-bone-formation-in-vitro
#19
Gregorio Raspini, Jan Wolff, Mika Helminen, Giovacchino Raspini, Mario Raspini, George K Sándor
Objectives: The aim of this study was to assess the interaction of a bioactive glass scaffold with cells derived from dental pulp, dental follicle and periodontal ligament. Material and Methods: Impacted third molars were surgically removed from three young donors. Cells from the dental pulp, follicle and periodontal ligament tissues were isolated and expanded. Different cell populations were characterised using specific CD markers. Expanded pulp, follicle and periodontal cells were then seeded onto bioactive glass scaffolds and cultured in osteogenic medium or basic medium...
January 2018: Journal of Oral & Maxillofacial Research
https://www.readbyqxmd.com/read/29703239/allogeneic-transplantation-of-mobilized-dental-pulp-stem-cells-with-the-mismatched-dog-leukocyte-antigen-type-is-safe-and-efficacious-for-total-pulp-regeneration
#20
Koichiro Iohara, Shinji Utsunomiya, Sakae Kohara, Misako Nakashima
BACKGROUND: We recently demonstrated that autologous transplantation of mobilized dental pulp stem cells (MDPSCs) was a safe and efficacious potential therapy for total pulp regeneration in a clinical study. The autologous MDPSCs, however, have some limitations to overcome, such as limited availability of discarded teeth from older patients. In the present study, we investigated whether MDPSCs can be used for allogeneic applications to expand their therapeutic use. METHODS: Analysis of dog leukocyte antigen (DLA) was performed using polymerase chain reaction from blood...
April 27, 2018: Stem Cell Research & Therapy
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