Mercedes Martín-Rufián, Renata Nascimento-Gomes, Ana Higuero, Amanda R Crisma, José A Campos-Sandoval, María C Gómez-García, Carolina Cardona, Tzuling Cheng, Carolina Lobo, Juan A Segura, Francisco J Alonso, Monika Szeliga, Jan Albrecht, Rui Curi, Javier Márquez, Alison Colquhoun, Ralph J Deberardinis, José M Matés
UNLABELLED: Mitochondrial glutaminase (GA) plays an essential role in cancer cell metabolism, contributing to biosynthesis, bioenergetics, and redox balance. Humans contain several GA isozymes encoded by the GLS and GLS2 genes, but the specific roles of each in cancer metabolism are still unclear. In this study, glioma SFxL and LN229 cells with silenced isoenzyme glutaminase KGA (encoded by GLS) showed lower survival ratios and a reduced GSH-dependent antioxidant capacity. These GLS-silenced cells also demonstrated induction of apoptosis indicated by enhanced annexin V binding capacity and caspase 3 activity...
March 2014: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"