Read by QxMD icon Read

Douglas AND Melton

Maayan Baron, Adrian Veres, Samuel L Wolock, Aubrey L Faust, Renaud Gaujoux, Amedeo Vetere, Jennifer Hyoje Ryu, Bridget K Wagner, Shai S Shen-Orr, Allon M Klein, Douglas A Melton, Itai Yanai
Although the function of the mammalian pancreas hinges on complex interactions of distinct cell types, gene expression profiles have primarily been described with bulk mixtures. Here we implemented a droplet-based, single-cell RNA-seq method to determine the transcriptomes of over 12,000 individual pancreatic cells from four human donors and two mouse strains. Cells could be divided into 15 clusters that matched previously characterized cell types: all endocrine cell types, including rare epsilon-cells; exocrine cell types; vascular cells; Schwann cells; quiescent and activated stellate cells; and four types of immune cells...
September 21, 2016: Cell Systems
Jeffrey R Millman, Chunhui Xie, Alana Van Dervort, Mads Gürtler, Felicia W Pagliuca, Douglas A Melton
No abstract text is available yet for this article.
2016: Nature Communications
Aaron R Cox, Ornella Barrandon, Erica P Cai, Jacqueline S Rios, Julia Chavez, Claire W Bonnyman, Carol J Lam, Peng Yi, Douglas A Melton, Jake A Kushner
The β-cell mitogenic effects of ANGPTL8 have been subjected to substantial debate. The original findings suggested that ANGPTL8 overexpression in mice induced a 17-fold increase in β-cell proliferation. Subsequent studies in mice contested this claim, but a more recent report in rats supported the original observations. These conflicting results might be explained by variable ANGPTL8 expression and differing methods of β-cell quantification. To resolve the controversy, three independent labs collaborated on a blinded study to test the effects of ANGPTL8 upon β-cell proliferation...
2016: PloS One
Jeffrey R Millman, Chunhui Xie, Alana Van Dervort, Mads Gürtler, Felicia W Pagliuca, Douglas A Melton
We recently reported the scalable in vitro production of functional stem cell-derived β-cells (SC-β cells). Here we extend this approach to generate the first SC-β cells from type 1 diabetic patients (T1D). β-cells are destroyed during T1D disease progression, making it difficult to extensively study them in the past. These T1D SC-β cells express β-cell markers, respond to glucose both in vitro and in vivo, prevent alloxan-induced diabetes in mice and respond to anti-diabetic drugs. Furthermore, we use an in vitro disease model to demonstrate the cells respond to different forms of β-cell stress...
2016: Nature Communications
Arturo J Vegas, Omid Veiseh, Mads Gürtler, Jeffrey R Millman, Felicia W Pagliuca, Andrew R Bader, Joshua C Doloff, Jie Li, Michael Chen, Karsten Olejnik, Hok Hei Tam, Siddharth Jhunjhunwala, Erin Langan, Stephanie Aresta-Dasilva, Srujan Gandham, James J McGarrigle, Matthew A Bochenek, Jennifer Hollister-Lock, Jose Oberholzer, Dale L Greiner, Gordon C Weir, Douglas A Melton, Robert Langer, Daniel G Anderson
No abstract text is available yet for this article.
April 2016: Nature Medicine
Douglas A Melton
Understanding the genes and signaling pathways that determine the differentiation and fate of a cell is a central goal of developmental biology. Using that information to gain mastery over the fates of cells presents new approaches to cell transplantation and drug discovery for human diseases including diabetes.
2016: Current Topics in Developmental Biology
Chaiyaboot Ariyachet, Alessio Tovaglieri, Guanjue Xiang, Jiaqi Lu, Manasvi S Shah, Camilla A Richmond, Catia Verbeke, Douglas A Melton, Ben Z Stanger, David Mooney, Ramesh A Shivdasani, Shaun Mahony, Qing Xia, David T Breault, Qiao Zhou
The gastrointestinal (GI) epithelium is a highly regenerative tissue with the potential to provide a renewable source of insulin(+) cells after undergoing cellular reprogramming. Here, we show that cells of the antral stomach have a previously unappreciated propensity for conversion into functional insulin-secreting cells. Native antral endocrine cells share a surprising degree of transcriptional similarity with pancreatic β cells, and expression of β cell reprogramming factors in vivo converts antral cells efficiently into insulin(+) cells with close molecular and functional similarity to β cells...
March 3, 2016: Cell Stem Cell
Wyona Freysteinson, Lisa Thomas, Amy Sebastian-Deutsch, Denika Douglas, Danielle Melton, Tania Celia, Kristin Reeves, Patricia Bowyer
PURPOSE: To describe the trajectory of viewing self in a mirror after an amputation and participants' perceptions of what health care professionals should know about mirrors. DESIGN: Hermeneutic phenomenology METHODS: Focus groups were conducted to collect the research data. FINDINGS: The mirror experience had three key moments: decision, seeing, and consent. The trajectory of viewing self in a mirror had four key themes: mirror shock, mirror anguish, recognizing self, and acceptance: a new normal...
February 1, 2016: Rehabilitation Nursing: the Official Journal of the Association of Rehabilitation Nurses
Arturo J Vegas, Omid Veiseh, Mads Gürtler, Jeffrey R Millman, Felicia W Pagliuca, Andrew R Bader, Joshua C Doloff, Jie Li, Michael Chen, Karsten Olejnik, Hok Hei Tam, Siddharth Jhunjhunwala, Erin Langan, Stephanie Aresta-Dasilva, Srujan Gandham, James J McGarrigle, Matthew A Bochenek, Jennifer Hollister-Lock, Jose Oberholzer, Dale L Greiner, Gordon C Weir, Douglas A Melton, Robert Langer, Daniel G Anderson
The transplantation of glucose-responsive, insulin-producing cells offers the potential for restoring glycemic control in individuals with diabetes. Pancreas transplantation and the infusion of cadaveric islets are currently implemented clinically, but these approaches are limited by the adverse effects of immunosuppressive therapy over the lifetime of the recipient and the limited supply of donor tissue. The latter concern may be addressed by recently described glucose-responsive mature beta cells that are derived from human embryonic stem cells (referred to as SC-β cells), which may represent an unlimited source of human cells for pancreas replacement therapy...
March 2016: Nature Medicine
Danny Ben-Zvi, Ornella Barrandon, Stephanie Hadley, Barak Blum, Quinn P Peterson, Douglas A Melton
Type 2 diabetes is characterized by a reduction in insulin function and an increase in glucagon activity that together result in hyperglycemia. Glucagon receptor antagonists have been developed as drugs for diabetes; however, they often increase glucagon plasma levels and induce the proliferation of glucagon-secreting α-cells. We find that the secreted protein Angiopoietin-like 4 (Angptl4) is up-regulated via Pparγ activation in white adipose tissue and plasma following an acute treatment with a glucagon receptor antagonist...
December 15, 2015: Proceedings of the National Academy of Sciences of the United States of America
Sundari Chetty, Elise N Engquist, Elie Mehanna, Kathy O Lui, Alexander M Tsankov, Douglas A Melton
Driving human pluripotent stem cells (hPSCs) into specific lineages is an inefficient and challenging process. We show that a potent Src inhibitor, PP1, regulates expression of genes involved in the G1 to S phase transition of the cell cycle, activates proteins in the retinoblastoma family, and subsequently increases the differentiation propensities of hPSCs into all three germ layers. We further demonstrate that genetic suppression of Src regulates the activity of the retinoblastoma protein and enhances the differentiation potential of hPSCs across all germ layers...
September 28, 2015: Journal of Cell Biology
Ewan M McNeil, Katy R Astell, Ann-Marie Ritchie, Steven Shave, Douglas R Houston, Preeti Bakrania, Hayley M Jones, Puneet Khurana, Claire Wallace, Tim Chapman, Martin A Wear, Malcolm D Walkinshaw, Barbara Saxty, David W Melton
ERCC1-XPF is a structure-specific endonuclease that is required for the repair of DNA lesions, generated by the widely used platinum-containing cancer chemotherapeutics such as cisplatin, through the Nucleotide Excision Repair and Interstrand Crosslink Repair pathways. Based on mouse xenograft experiments, where ERCC1-deficient melanomas were cured by cisplatin therapy, we proposed that inhibition of ERCC1-XPF could enhance the effectiveness of platinum-based chemotherapy. Here we report the identification and properties of inhibitors against two key targets on ERCC1-XPF...
July 2015: DNA Repair
Jennifer H R Kenty, Douglas A Melton
Studying the response of islet cells to glucose stimulation is important for understanding cell function in healthy and disease states. Most functional assays are performed on whole islets or cell populations, resulting in averaged observations and loss of information at the single cell level. We demonstrate methods to examine calcium fluxing in individual cells of intact islets in response to multiple glucose challenges. Wild-type mouse islets predominantly contained cells that responded to three (out of three) sequential high glucose challenges, whereas cells of diabetic islets (db/db or NOD) responded less frequently or not at all...
2015: PloS One
Danny Ben-Zvi, Douglas A Melton
Nutrition presents unanswered scientific questions of high public health importance. We envision model systems composed of interacting gastrointestinal and metabolic tissues derived from human embryonic stem cells, populated by gut microbiota. The culture will be embedded in 3D scaffolds, creating a controlled experimental system that enables tissue sampling and imaging.
March 26, 2015: Cell
Wilson Leung, Christopher D Shaffer, Laura K Reed, Sheryl T Smith, William Barshop, William Dirkes, Matthew Dothager, Paul Lee, Jeannette Wong, David Xiong, Han Yuan, James E J Bedard, Joshua F Machone, Seantay D Patterson, Amber L Price, Bryce A Turner, Srebrenka Robic, Erin K Luippold, Shannon R McCartha, Tezin A Walji, Chelsea A Walker, Kenneth Saville, Marita K Abrams, Andrew R Armstrong, William Armstrong, Robert J Bailey, Chelsea R Barberi, Lauren R Beck, Amanda L Blaker, Christopher E Blunden, Jordan P Brand, Ethan J Brock, Dana W Brooks, Marie Brown, Sarah C Butzler, Eric M Clark, Nicole B Clark, Ashley A Collins, Rebecca J Cotteleer, Peterson R Cullimore, Seth G Dawson, Carter T Docking, Sasha L Dorsett, Grace A Dougherty, Kaitlyn A Downey, Andrew P Drake, Erica K Earl, Trevor G Floyd, Joshua D Forsyth, Jonathan D Foust, Spencer L Franchi, James F Geary, Cynthia K Hanson, Taylor S Harding, Cameron B Harris, Jonathan M Heckman, Heather L Holderness, Nicole A Howey, Dontae A Jacobs, Elizabeth S Jewell, Maria Kaisler, Elizabeth A Karaska, James L Kehoe, Hannah C Koaches, Jessica Koehler, Dana Koenig, Alexander J Kujawski, Jordan E Kus, Jennifer A Lammers, Rachel R Leads, Emily C Leatherman, Rachel N Lippert, Gregory S Messenger, Adam T Morrow, Victoria Newcomb, Haley J Plasman, Stephanie J Potocny, Michelle K Powers, Rachel M Reem, Jonathan P Rennhack, Katherine R Reynolds, Lyndsey A Reynolds, Dong K Rhee, Allyson B Rivard, Adam J Ronk, Meghan B Rooney, Lainey S Rubin, Luke R Salbert, Rasleen K Saluja, Taylor Schauder, Allison R Schneiter, Robert W Schulz, Karl E Smith, Sarah Spencer, Bryant R Swanson, Melissa A Tache, Ashley A Tewilliager, Amanda K Tilot, Eve VanEck, Matthew M Villerot, Megan B Vylonis, David T Watson, Juliana A Wurzler, Lauren M Wysocki, Monica Yalamanchili, Matthew A Zaborowicz, Julia A Emerson, Carlos Ortiz, Frederic J Deuschle, Lauren A DiLorenzo, Katie L Goeller, Christopher R Macchi, Sarah E Muller, Brittany D Pasierb, Joseph E Sable, Jessica M Tucci, Marykathryn Tynon, David A Dunbar, Levent H Beken, Alaina C Conturso, Benjamin L Danner, Gabriella A DeMichele, Justin A Gonzales, Maureen S Hammond, Colleen V Kelley, Elisabeth A Kelly, Danielle Kulich, Catherine M Mageeney, Nikie L McCabe, Alyssa M Newman, Lindsay A Spaeder, Richard A Tumminello, Dennis Revie, Jonathon M Benson, Michael C Cristostomo, Paolo A DaSilva, Katherine S Harker, Jenifer N Jarrell, Luis A Jimenez, Brandon M Katz, William R Kennedy, Kimberly S Kolibas, Mark T LeBlanc, Trung T Nguyen, Daniel S Nicolas, Melissa D Patao, Shane M Patao, Bryan J Rupley, Bridget J Sessions, Jennifer A Weaver, Anya L Goodman, Erica L Alvendia, Shana M Baldassari, Ashley S Brown, Ian O Chase, Maida Chen, Scott Chiang, Avery B Cromwell, Ashley F Custer, Tia M DiTommaso, Jad El-Adaimi, Nora C Goscinski, Ryan A Grove, Nestor Gutierrez, Raechel S Harnoto, Heather Hedeen, Emily L Hong, Barbara L Hopkins, Vilma F Huerta, Colin Khoshabian, Kristin M LaForge, Cassidy T Lee, Benjamin M Lewis, Anniken M Lydon, Brian J Maniaci, Ryan D Mitchell, Elaine V Morlock, William M Morris, Priyanka Naik, Nicole C Olson, Jeannette M Osterloh, Marcos A Perez, Jonathan D Presley, Matt J Randazzo, Melanie K Regan, Franca G Rossi, Melanie A Smith, Eugenia A Soliterman, Ciani J Sparks, Danny L Tran, Tiffany Wan, Anne A Welker, Jeremy N Wong, Aparna Sreenivasan, Jim Youngblom, Andrew Adams, Justin Alldredge, Ashley Bryant, David Carranza, Alyssa Cifelli, Kevin Coulson, Calise Debow, Noelle Delacruz, Charlene Emerson, Cassandra Farrar, Don Foret, Edgar Garibay, John Gooch, Michelle Heslop, Sukhjit Kaur, Ambreen Khan, Van Kim, Travis Lamb, Peter Lindbeck, Gabi Lucas, Elizabeth Macias, Daniela Martiniuc, Lissett Mayorga, Joseph Medina, Nelson Membreno, Shady Messiah, Lacey Neufeld, San Francisco Nguyen, Zachary Nichols, George Odisho, Daymon Peterson, Laura Rodela, Priscilla Rodriguez, Vanessa Rodriguez, Jorge Ruiz, Will Sherrill, Valeria Silva, Jeri Sparks, Geeta Statton, Ashley Townsend, Isabel Valdez, Mary Waters, Kyle Westphal, Stacey Winkler, Joannee Zumkehr, Randall J DeJong, Arlene J Hoogewerf, Cheri M Ackerman, Isaac O Armistead, Lara Baatenburg, Matthew J Borr, Lindsay K Brouwer, Brandon J Burkhart, Kelsey T Bushhouse, Lejla Cesko, Tiffany Y Y Choi, Heather Cohen, Amanda M Damsteegt, Jess M Darusz, Cory M Dauphin, Yelena P Davis, Emily J Diekema, Melissa Drewry, Michelle E M Eisen, Hayley M Faber, Katherine J Faber, Elizabeth Feenstra, Isabella T Felzer-Kim, Brandy L Hammond, Jesse Hendriksma, Milton R Herrold, Julia A Hilbrands, Emily J Howell, Sarah A Jelgerhuis, Timothy R Jelsema, Benjamin K Johnson, Kelly K Jones, Anna Kim, Ross D Kooienga, Erika E Menyes, Eric A Nollet, Brittany E Plescher, Lindsay Rios, Jenny L Rose, Allison J Schepers, Geoff Scott, Joshua R Smith, Allison M Sterling, Jenna C Tenney, Chris Uitvlugt, Rachel E VanDyken, Marielle VanderVennen, Samantha Vue, Nighat P Kokan, Kwabea Agbley, Sampson K Boham, Daniel Broomfield, Kayla Chapman, Ali Dobbe, Ian Dobbe, William Harrington, Marwan Ibrahem, Andre Kennedy, Chad A Koplinsky, Cassandra Kubricky, Danielle Ladzekpo, Claire Pattison, Roman E Ramirez, Lucia Wande, Sarah Woehlke, Matthew Wawersik, Elizabeth Kiernan, Jeffrey S Thompson, Roxanne Banker, Justina R Bartling, Chinmoy I Bhatiya, Anna L Boudoures, Lena Christiansen, Daniel S Fosselman, Kristin M French, Ishwar S Gill, Jessen T Havill, Jaelyn L Johnson, Lauren J Keny, John M Kerber, Bethany M Klett, Christina N Kufel, Francis J May, Jonathan P Mecoli, Callie R Merry, Lauren R Meyer, Emily G Miller, Gregory J Mullen, Katherine C Palozola, Jacob J Pfeil, Jessica G Thomas, Evan M Verbofsky, Eric P Spana, Anant Agarwalla, Julia Chapman, Ben Chlebina, Insun Chong, I N Falk, John D Fitzgibbons, Harrison Friedman, Osagie Ighile, Andrew J Kim, Kristin A Knouse, Faith Kung, Danny Mammo, Chun Leung Ng, Vinayak S Nikam, Diana Norton, Philip Pham, Jessica W Polk, Shreya Prasad, Helen Rankin, Camille D Ratliff, Victoria Scala, Nicholas U Schwartz, Jessica A Shuen, Amy Xu, Thomas Q Xu, Yi Zhang, Anne G Rosenwald, Martin G Burg, Stephanie J Adams, Morgan Baker, Bobbi Botsford, Briana Brinkley, Carter Brown, Shadie Emiah, Erica Enoch, Chad Gier, Alyson Greenwell, Lindsay Hoogenboom, Jordan E Matthews, Mitchell McDonald, Amanda Mercer, Nicholaus Monsma, Kristine Ostby, Alen Ramic, Devon Shallman, Matthew Simon, Eric Spencer, Trisha Tomkins, Pete Wendland, Anna Wylie, Michael J Wolyniak, Gregory M Robertson, Samuel I Smith, Justin R DiAngelo, Eric D Sassu, Satish C Bhalla, Karim A Sharif, Tenzin Choeying, Jason S Macias, Fareed Sanusi, Karvyn Torchon, April E Bednarski, Consuelo J Alvarez, Kristen C Davis, Carrie A Dunham, Alaina J Grantham, Amber N Hare, Jennifer Schottler, Zackary W Scott, Gary A Kuleck, Nicole S Yu, Marian M Kaehler, Jacob Jipp, Paul J Overvoorde, Elizabeth Shoop, Olivia Cyrankowski, Betsy Hoover, Matt Kusner, Devry Lin, Tijana Martinov, Jonathan Misch, Garrett Salzman, Holly Schiedermayer, Michael Snavely, Stephanie Zarrasola, Susan Parrish, Atlee Baker, Alissa Beckett, Carissa Belella, Julie Bryant, Turner Conrad, Adam Fearnow, Carolina Gomez, Robert A Herbstsomer, Sarah Hirsch, Christen Johnson, Melissa Jones, Rita Kabaso, Eric Lemmon, Carolina Marques Dos Santos Vieira, Darryl McFarland, Christopher McLaughlin, Abbie Morgan, Sepo Musokotwane, William Neutzling, Jana Nietmann, Christina Paluskievicz, Jessica Penn, Emily Peoples, Caitlin Pozmanter, Emily Reed, Nichole Rigby, Lasse Schmidt, Micah Shelton, Rebecca Shuford, Tiara Tirasawasdichai, Blair Undem, Damian Urick, Kayla Vondy, Bryan Yarrington, Todd T Eckdahl, Jeffrey L Poet, Alica B Allen, John E Anderson, Jason M Barnett, Jordan S Baumgardner, Adam D Brown, Jordan E Carney, Ramiro A Chavez, Shelbi L Christgen, Jordan S Christie, Andrea N Clary, Michel A Conn, Kristen M Cooper, Matt J Crowley, Samuel T Crowley, Jennifer S Doty, Brian A Dow, Curtis R Edwards, Darcie D Elder, John P Fanning, Bridget M Janssen, Anthony K Lambright, Curtiss E Lane, Austin B Limle, Tammy Mazur, Marly R McCracken, Alexa M McDonough, Amy D Melton, Phillip J Minnick, Adam E Musick, William H Newhart, Joseph W Noynaert, Bradley J Ogden, Michael W Sandusky, Samantha M Schmuecker, Anna L Shipman, Anna L Smith, Kristen M Thomsen, Matthew R Unzicker, William B Vernon, Wesley W Winn, Dustin S Woyski, Xiao Zhu, Chunguang Du, Caitlin Ament, Soham Aso, Laura Simone Bisogno, Jason Caronna, Nadezhda Fefelova, Lenin Lopez, Lorraine Malkowitz, Jonathan Marra, Daniella Menillo, Ifeanyi Obiorah, Eric Nyabeta Onsarigo, Shekerah Primus, Mahdi Soos, Archana Tare, Ameer Zidan, Christopher J Jones, Todd Aronhalt, James M Bellush, Christa Burke, Steve DeFazio, Benjamin R Does, Todd D Johnson, Nicholas Keysock, Nelson H Knudsen, James Messler, Kevin Myirski, Jade Lea Rekai, Ryan Michael Rempe, Michael S Salgado, Erica Stagaard, Justin R Starcher, Andrew W Waggoner, Anastasia K Yemelyanova, Amy T Hark, Anne Bertolet, Cyrus E Kuschner, Kesley Parry, Michael Quach, Lindsey Shantzer, Mary E Shaw, Mary A Smith, Omolara Glenn, Portia Mason, Charlotte Williams, S Catherine Silver Key, Tyneshia C P Henry, Ashlee G Johnson, Jackie X White, Adam Haberman, Sam Asinof, Kelly Drumm, Trip Freeburg, Nadia Safa, Darrin Schultz, Yakov Shevin, Petros Svoronos, Tam Vuong, Jules Wellinghoff, Laura L M Hoopes, Kim M Chau, Alyssa Ward, E Gloria C Regisford, LaJerald Augustine, Brionna Davis-Reyes, Vivienne Echendu, Jasmine Hales, Sharon Ibarra, Lauriaun Johnson, Steven Ovu, John M Braverman, Thomas J Bahr, Nicole M Caesar, Christopher Campana, Daniel W Cassidy, Peter A Cognetti, Johnathan D English, Matthew C Fadus, Cameron N Fick, Philip J Freda, Bryan M Hennessy, Kelsey Hockenberger, Jennifer K Jones, Jessica E King, Christopher R Knob, Karen J Kraftmann, Linghui Li, Lena N Lupey, Carl J Minniti, Thomas F Minton, Joseph V Moran, Krishna Mudumbi, Elizabeth C Nordman, William J Puetz, Lauren M Robinson, Thomas J Rose, Edward P Sweeney, Ashley S Timko, Don W Paetkau, Heather L Eisler, Megan E Aldrup, Jessica M Bodenberg, Mara G Cole, Kelly M Deranek, Megan DeShetler, Rose M Dowd, Alexandra K Eckardt, Sharon C Ehret, Jessica Fese, Amanda D Garrett, Anna Kammrath, Michelle L Kappes, Morgan R Light, Anne C Meier, Allison O'Rouke, Mallory Perella, Kimberley Ramsey, Jennifer R Ramthun, Mary T Reilly, Deirdre Robinett, Nadine L Rossi, Mary Grace Schueler, Emma Shoemaker, Kristin M Starkey, Ashley Vetor, Abby Vrable, Vidya Chandrasekaran, Christopher Beck, Kristen R Hatfield, Douglas A Herrick, Christopher B Khoury, Charlotte Lea, Christopher A Louie, Shannon M Lowell, Thomas J Reynolds, Jeanine Schibler, Alexandra H Scoma, Maxwell T Smith-Gee, Sarah Tuberty, Christopher D Smith, Jane E Lopilato, Jeanette Hauke, Jennifer A Roecklein-Canfield, Maureen Corrielus, Hannah Gilman, Stephanie Intriago, Amanda Maffa, Sabya A Rauf, Katrina Thistle, Melissa Trieu, Jenifer Winters, Bib Yang, Charles R Hauser, Tariq Abusheikh, Yara Ashrawi, Pedro Benitez, Lauren R Boudreaux, Megan Bourland, Miranda Chavez, Samantha Cruz, GiNell Elliott, Jesse R Farek, Sarah Flohr, Amanda H Flores, Chelsey Friedrichs, Zach Fusco, Zane Goodwin, Eric Helmreich, John Kiley, John Mark Knepper, Christine Langner, Megan Martinez, Carlos Mendoza, Monal Naik, Andrea Ochoa, Nicolas Ragland, England Raimey, Sunil Rathore, Evangelina Reza, Griffin Sadovsky, Marie-Isabelle B Seydoux, Jonathan E Smith, Anna K Unruh, Vicente Velasquez, Matthew W Wolski, Yuying Gosser, Shubha Govind, Nicole Clarke-Medley, Leslie Guadron, Dawn Lau, Alvin Lu, Cheryl Mazzeo, Mariam Meghdari, Simon Ng, Brad Pamnani, Olivia Plante, Yuki Kwan Wa Shum, Roy Song, Diana E Johnson, Mai Abdelnabi, Alexi Archambault, Norma Chamma, Shailly Gaur, Deborah Hammett, Adrese Kandahari, Guzal Khayrullina, Sonali Kumar, Samantha Lawrence, Nigel Madden, Max Mandelbaum, Heather Milnthorp, Shiv Mohini, Roshni Patel, Sarah J Peacock, Emily Perling, Amber Quintana, Michael Rahimi, Kristen Ramirez, Rishi Singhal, Corinne Weeks, Tiffany Wong, Aubree T Gillis, Zachary D Moore, Christopher D Savell, Reece Watson, Stephanie F Mel, Arjun A Anilkumar, Paul Bilinski, Rostislav Castillo, Michael Closser, Nathalia M Cruz, Tiffany Dai, Giancarlo F Garbagnati, Lanor S Horton, Dongyeon Kim, Joyce H Lau, James Z Liu, Sandy D Mach, Thu A Phan, Yi Ren, Kenneth E Stapleton, Jean M Strelitz, Ray Sunjed, Joyce Stamm, Morgan C Anderson, Bethany Grace Bonifield, Daniel Coomes, Adam Dillman, Elaine J Durchholz, Antoinette E Fafara-Thompson, Meleah J Gross, Amber M Gygi, Lesley E Jackson, Amy Johnson, Zuzana Kocsisova, Joshua L Manghelli, Kylie McNeil, Michael Murillo, Kierstin L Naylor, Jessica Neely, Emmy E Ogawa, Ashley Rich, Anna Rogers, J Devin Spencer, Kristina M Stemler, Allison A Throm, Matt Van Camp, Katie Weihbrecht, T Aaron Wiles, Mallory A Williams, Matthew Williams, Kyle Zoll, Cheryl Bailey, Leming Zhou, Darla M Balthaser, Azita Bashiri, Mindy E Bower, Kayla A Florian, Nazanin Ghavam, Elizabeth S Greiner-Sosanko, Helmet Karim, Victor W Mullen, Carly E Pelchen, Paul M Yenerall, Jiayu Zhang, Michael R Rubin, Suzette M Arias-Mejias, Armando G Bermudez-Capo, Gabriela V Bernal-Vega, Mariela Colon-Vazquez, Arelys Flores-Vazquez, Mariela Gines-Rosario, Ivan G Llavona-Cartagena, Javier O Martinez-Rodriguez, Lionel Ortiz-Fuentes, Eliezer O Perez-Colomba, Joseph Perez-Otero, Elisandra Rivera, Luke J Rodriguez-Giron, Arnaldo J Santiago-Sanabria, Andrea M Senquiz-Gonzalez, Frank R Soto delValle, Dorianmarie Vargas-Franco, Karla I Velázquez-Soto, Joan D Zambrana-Burgos, Juan Carlos Martinez-Cruzado, Lillyann Asencio-Zayas, Kevin Babilonia-Figueroa, Francis D Beauchamp-Pérez, Juliana Belén-Rodríguez, Luciann Bracero-Quiñones, Andrea P Burgos-Bula, Xavier A Collado-Méndez, Luis R Colón-Cruz, Ana I Correa-Muller, Jonathan L Crooke-Rosado, José M Cruz-García, Marianna Defendini-Ávila, Francheska M Delgado-Peraza, Alex J Feliciano-Cancela, Valerie M Gónzalez-Pérez, Wilfried Guiblet, Aldo Heredia-Negrón, Jennifer Hernández-Muñiz, Lourdes N Irizarry-González, Ángel L Laboy-Corales, Gabriela A Llaurador-Caraballo, Frances Marín-Maldonado, Ulises Marrero-Llerena, Héctor A Martell-Martínez, Idaliz M Martínez-Traverso, Kiara N Medina-Ortega, Sonya G Méndez-Castellanos, Krizia C Menéndez-Serrano, Carol I Morales-Caraballo, Saryleine Ortiz-DeChoudens, Patricia Ortiz-Ortiz, Hendrick Pagán-Torres, Diana Pérez-Afanador, Enid M Quintana-Torres, Edwin G Ramírez-Aponte, Carolina Riascos-Cuero, Michelle S Rivera-Llovet, Ingrid T Rivera-Pagán, Ramón E Rivera-Vicéns, Fabiola Robles-Juarbe, Lorraine Rodríguez-Bonilla, Brian O Rodríguez-Echevarría, Priscila M Rodríguez-García, Abneris E Rodríguez-Laboy, Susana Rodríguez-Santiago, Michael L Rojas-Vargas, Eva N Rubio-Marrero, Albeliz Santiago-Colón, Jorge L Santiago-Ortiz, Carlos E Santos-Ramos, Joseline Serrano-González, Alina M Tamayo-Figueroa, Edna P Tascón-Peñaranda, José L Torres-Castillo, Nelson A Valentín-Feliciano, Yashira M Valentín-Feliciano, Nadyan M Vargas-Barreto, Miguel Vélez-Vázquez, Luis R Vilanova-Vélez, Cristina Zambrana-Echevarría, Christy MacKinnon, Hui-Min Chung, Chris Kay, Anthony Pinto, Olga R Kopp, Joshua Burkhardt, Chris Harward, Robert Allen, Pavan Bhat, Jimmy Hsiang-Chun Chang, York Chen, Christopher Chesley, Dara Cohn, David DuPuis, Michael Fasano, Nicholas Fazzio, Katherine Gavinski, Heran Gebreyesus, Thomas Giarla, Marcus Gostelow, Rachel Greenstein, Hashini Gunasinghe, Casey Hanson, Amanda Hay, Tao Jian He, Katie Homa, Ruth Howe, Jeff Howenstein, Henry Huang, Aaditya Khatri, Young Lu Kim, Olivia Knowles, Sarah Kong, Rebecca Krock, Matt Kroll, Julia Kuhn, Matthew Kwong, Brandon Lee, Ryan Lee, Kevin Levine, Yedda Li, Bo Liu, Lucy Liu, Max Liu, Adam Lousararian, Jimmy Ma, Allyson Mallya, Charlie Manchee, Joseph Marcus, Stephen McDaniel, Michelle L Miller, Jerome M Molleston, Cristina Montero Diez, Patrick Ng, Natalie Ngai, Hien Nguyen, Andrew Nylander, Jason Pollack, Suchita Rastogi, Himabindu Reddy, Nathaniel Regenold, Jon Sarezky, Michael Schultz, Jien Shim, Tara Skorupa, Kenneth Smith, Sarah J Spencer, Priya Srikanth, Gabriel Stancu, Andrew P Stein, Marshall Strother, Lisa Sudmeier, Mengyang Sun, Varun Sundaram, Noor Tazudeen, Alan Tseng, Albert Tzeng, Rohit Venkat, Sandeep Venkataram, Leah Waldman, Tracy Wang, Hao Yang, Jack Y Yu, Yin Zheng, Mary L Preuss, Angelica Garcia, Matt Juergens, Robert W Morris, Alexis A Nagengast, Julie Azarewicz, Thomas J Carr, Nicole Chichearo, Mike Colgan, Megan Donegan, Bob Gardner, Nik Kolba, Janice L Krumm, Stacey Lytle, Laurell MacMillian, Mary Miller, Andrew Montgomery, Alysha Moretti, Brittney Offenbacker, Mike Polen, John Toth, John Woytanowski, Lisa Kadlec, Justin Crawford, Mary L Spratt, Ashley L Adams, Brianna K Barnard, Martin N Cheramie, Anne M Eime, Kathryn L Golden, Allyson P Hawkins, Jessica E Hill, Jessica A Kampmeier, Cody D Kern, Emily E Magnuson, Ashley R Miller, Cody M Morrow, Julia C Peairs, Gentry L Pickett, Sarah A Popelka, Alexis J Scott, Emily J Teepe, Katie A TerMeer, Carmen A Watchinski, Lucas A Watson, Rachel E Weber, Kate A Woodard, Daron C Barnard, Isaac Appiah, Michelle M Giddens, Gerard P McNeil, Adeola Adebayo, Kate Bagaeva, Justina Chinwong, Chrystel Dol, Eunice George, Kirk Haltaufderhyde, Joanna Haye, Manpreet Kaur, Max Semon, Dmitri Serjanov, Anika Toorie, Christopher Wilson, Nicole C Riddle, Jeremy Buhler, Elaine R Mardis, Sarah C R Elgin
The Muller F element (4.2 Mb, ~80 protein-coding genes) is an unusual autosome of Drosophila melanogaster; it is mostly heterochromatic with a low recombination rate. To investigate how these properties impact the evolution of repeats and genes, we manually improved the sequence and annotated the genes on the D. erecta, D. mojavensis, and D. grimshawi F elements and euchromatic domains from the Muller D element. We find that F elements have greater transposon density (25-50%) than euchromatic reference regions (3-11%)...
May 2015: G3: Genes—Genomes—Genetics
Jonas Ahnfelt-Rønne, Ole D Madsen
Regenerative therapy in diabetes with the capacity to reconstitute a functional β-cell mass sufficient for glycemic control holds the promise to effectively prevent the development of devastating late complications due to the unique ability of the β-cell to sense and regulate blood-glucose levels. An ability that cannot be mimicked by insulin replacement therapy or any other means of current treatment regiments for very large patient populations. Recently, Douglas A. Melton's group from Harvard University reported the identification of a circulating protein secreted from the liver under insulin resistant states which is sufficient to dramatically and specifically increase the replication rate of β-cells in the mouse resulting in an increased functional β-cell mass over time...
2014: Islets
Douglas Melton, Calvin D Lewis, Nathan E Price, Kent S Gates
Hydralazine (4) is an antihypertensive agent that displays both mutagenic and epigenetic properties. Here, gel electrophoretic, mass spectroscopic, and chemical kinetics methods were used to provide evidence that medicinally relevant concentrations of 4 rapidly form covalent adducts with abasic sites in double- and single-stranded DNA under physiological conditions. These findings raise the intriguing possibility that the genotoxic properties of this clinically used drug arise via reactions with an endogenous DNA lesion rather than with the canonical structure of DNA...
December 15, 2014: Chemical Research in Toxicology
Felicia W Pagliuca, Jeffrey R Millman, Mads Gürtler, Michael Segel, Alana Van Dervort, Jennifer Hyoje Ryu, Quinn P Peterson, Dale Greiner, Douglas A Melton
The generation of insulin-producing pancreatic β cells from stem cells in vitro would provide an unprecedented cell source for drug discovery and cell transplantation therapy in diabetes. However, insulin-producing cells previously generated from human pluripotent stem cells (hPSC) lack many functional characteristics of bona fide β cells. Here, we report a scalable differentiation protocol that can generate hundreds of millions of glucose-responsive β cells from hPSC in vitro. These stem-cell-derived β cells (SC-β) express markers found in mature β cells, flux Ca(2+) in response to glucose, package insulin into secretory granules, and secrete quantities of insulin comparable to adult β cells in response to multiple sequential glucose challenges in vitro...
October 9, 2014: Cell
Barak Blum, Adam N Roose, Ornella Barrandon, René Maehr, Anthony C Arvanites, Lance S Davidow, Jeffrey C Davis, Quinn P Peterson, Lee L Rubin, Douglas A Melton
Dysfunction or death of pancreatic β cells underlies both types of diabetes. This functional decline begins with β cell stress and de-differentiation. Current drugs for type 2 diabetes (T2D) lower blood glucose levels but they do not directly alleviate β cell stress nor prevent, let alone reverse, β cell de-differentiation. We show here that Urocortin 3 (Ucn3), a marker for mature β cells, is down-regulated in the early stages of T2D in mice and when β cells are stressed in vitro. Using an insulin expression-coupled lineage tracer, with Ucn3 as a reporter for the mature β cell state, we screen for factors that reverse β cell de-differentiation...
2014: ELife
Jonas Ahnfelt-Rønne, Ole D Madsen
Regenerative therapy in diabetes with the capacity to reconstitute a functional β-cell mass sufficient for glycemic control holds the promise to effectively prevent the development of devastating late complications due to the unique ability of the β-cell to sense and regulate blood-glucose levels. An ability that cannot be mimicked by insulin replacement therapy or any other means of current treatment regiments for very large patient populations. Recently, Douglas A. Melton's group from Harvard University reported the identification of a circulating protein secreted from the liver under insulin resistant states which is sufficient to dramatically and specifically increase the replication rate of β-cells in the mouse resulting in an increased functional β-cell mass over time...
May 5, 2014: Islets
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"