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https://www.readbyqxmd.com/read/28087912/-t-cell-receptor-%C3%AE-chain-cdr3-spectratyping-and-cytomegalovirus-activation-in-allogeneic-hematopoietic-stem-cell-transplant-recipients
#1
Zhihua Wu, Min Jing, Hanying Liang, Rong Yang, Yaping Huang, Xiaoming Chen, Jianhua Hu, Jun Fan
Objective: To explore the association between T-cell receptor beta variable (TCR BV) complementarity determining region 3 (CDR3) spectratyping and CMV activation in the recipients of allogeneic hematopoietic stem cell transplantation (HSCT). Methods: Fluorescence quantitative PCR melting curve analysis was used to sequence 24 TCR BV families in 7 HSCT recipients and 3 healthy controls. CMV-pp65 antigenemia was measured by immunohistochemical staining. Plasma IgM specific for CMV was identified using ELISA. Relationship between TCR BV families and CMV activation was statistically analyzed...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/28087496/digital-pills-to-measure-opioid-ingestion-patterns-in-emergency-department-patients-with-acute-fracture-pain-a-pilot-study
#2
Peter R Chai, Stephanie Carreiro, Brendan J Innes, Rochelle K Rosen, Conall O'Cleirigh, Kenneth H Mayer, Edward W Boyer
BACKGROUND: Nonadherence to prescribed regimens for opioid analgesic agents contributes to increasing opioid abuse and overdose death. Opioids are frequently prescribed on an as-needed basis, placing the responsibility to determine opioid dose and frequency with the patient. There is wide variability in physician prescribing patterns because of the lack of data describing how patients actually use as-needed opioid analgesics. Digital pill systems have a radiofrequency emitter that directly measures medication ingestion events, and they provide an opportunity to discover the dose, timing, and duration of opioid therapy...
January 13, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28087416/the-path-of-a-click-stimulus-from-ear-canal-to-umbo
#3
Mario Milazzo, Elika Fallah, Michael Carapezza, Nina S Kumar, Jason H Lei, Elizabeth S Olson
The tympanic membrane (TM) has a key role in transmitting sounds to the inner ear, but a concise description of how the TM performs this function remains elusive. This paper probes TM operation by applying a free field click stimulus to the gerbil ear and exploring the consequent motions of the TM and umbo. Motions of the TM were measured both on radial tracks starting close to the umbo and on a grid distal and adjacent to the umbo. The experimental results confirmed the high fidelity of sound transmission from the ear canal to the umbo...
January 10, 2017: Hearing Research
https://www.readbyqxmd.com/read/28086851/efficacy-of-intermittent-theta-burst-stimulation-itbs-and-10-hz-high-frequency-repetitive-transcranial-magnetic-stimulation-rtms-in-treatment-resistant-unipolar-depression-study-protocol-for-a-randomised-controlled-trial
#4
Samuel Bulteau, Veronique Sébille, Guillemette Fayet, Veronique Thomas-Ollivier, Thibault Deschamps, Annabelle Bonnin-Rivalland, Edouard Laforgue, Anne Pichot, Pierre Valrivière, Elisabeth Auffray-Calvier, June Fortin, Yann Péréon, Jean-Marie Vanelle, Anne Sauvaget
BACKGROUND: The treatment of depression remains a challenge since at least 40% of patients do not respond to initial antidepressant therapy and 20% present chronic symptoms (more than 2 years despite standard treatment administered correctly). Repetitive transcranial magnetic stimulation (rTMS) is an effective adjuvant therapy but still not ideal. Intermittent Theta Burst Stimulation (iTBS), which has only been used recently in clinical practice, could have a faster and more intense effect compared to conventional protocols, including 10-Hz high-frequency rTMS (HF-rTMS)...
January 13, 2017: Trials
https://www.readbyqxmd.com/read/28086814/intestinal-helminth-co-infection-and-associated-factors-among-tuberculosis-patients-in-arba-minch-ethiopia
#5
Getaneh Alemu, Mohammedaman Mama
BACKGROUND: Helminths affect the outcome of tuberculosis by shifting cell mediated immune response to humoral and by total suppression of the host immune system. On the reverse, Mycobacterium infection favors immune escape of helminths. Therefore assessing helminth co-infection rate and predisposing factors in tuberculosis patients is mandatory to set strategies for better case management. METHODS: Facility based cross-sectional study was conducted in Arba Minch to assess the prevalence and associated factors of intestinal helminths among pulmonary tuberculosis patients from January to August, 2016...
January 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28080987/ploidy-dynamics-and-evolvability-in-fungi
#6
REVIEW
Noa Blutraich Wertheimer, Neil Stone, Judith Berman
Rapid responses to acute stresses are essential for stress survival and are critical to the ability of fungal pathogens to adapt to new environments or hosts. The rapid emergence of drug resistance is used as a model for how fungi adapt and survive stress conditions that inhibit the growth of progenitor cells. Aneuploidy and loss of heterozygosity (LOH), which are large-scale genome shifts involving whole chromosomes or chromosome arms, occur at higher frequency than point mutations and have the potential to mediate stress survival...
December 5, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28079524/spatial-heterogeneity-in-the-time-and-frequency-properties-of-skin-perfusion
#7
Michele Sorelli, Zlatka Stoyneva, Irina Mizeva, Leonardo Bocchi
Pathological alterations of the microcirculatory system can be identified by measuring the temporal and spectral properties of Laser Doppler Flowmetry (LDF) signals acquired on the skin, and their changes following physiological stimulation. A wide range of stimulation protocols and measurement locations are observed in literature. Researchers often use non-invasive stimulation techniques, such as post-occlusive hyperaemia, cold tests, and local heating. As concerns the stimulation/recording sites, the forearm, fingers, and toes are typically selected to conduct microcirculation studies...
January 12, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28077662/stability-and-plasticity-in-neural-encoding-of-linguistically-relevant-pitch-patterns
#8
Zilong Xie, Rachel Denise Reetzke, Bharath Chandrasekaran
While life-long language experience modulates subcortical encoding of pitch patterns, there is emerging evidence that short-term training introduced in adulthood also shapes subcortical pitch encoding. Here, we use a cross-language design to examine the stability of language experience-dependent subcortical plasticity over multiple days. We then examine the extent to which behavioral relevance induced by speech sound-to-category training leads to plastic changes in subcortical pitch encoding in adulthood relative to adolescence, a period of ongoing maturation of subcortical and cortical auditory processing...
January 11, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28077588/first-in-human-evaluation-of-the-safety-and-immunogenicity-of-an-intranasally-administered-replication-competent-sendai-virus-vectored-hiv-type-1-gag-vaccine-induction-of-potent-t-cell-or-antibody-responses-in-prime-boost-regimens
#9
Julien Nyombayire, Omu Anzala, Brian Gazzard, Etienne Karita, Philip Bergin, Peter Hayes, Jakub Kopycinski, Gloria Omosa-Manyonyi, Akil Jackson, Jean Bizimana, Bashir Farah, Eddy Sayeed, Christopher L Parks, Makoto Inoue, Takashi Hironaka, Hiroto Hara, Tsugumine Shu, Tetsuro Matano, Len Dally, Burc Barin, Harriet Park, Jill Gilmour, Angela Lombardo, Jean-Louis Excler, Patricia Fast, Dagna S Laufer, Josephine H Cox
BACKGROUND:  We report the first-in-human safety and immunogenicity assessment of a prototype intranasally administered, replication-competent Sendai virus (SeV)-vectored, human immunodeficiency virus type 1 (HIV-1) vaccine. METHODS:  Sixty-five HIV-1-uninfected adults in Kenya, Rwanda, and the United Kingdom were assigned to receive 1 of 4 prime-boost regimens (administered at 0 and 4 months, respectively; ratio of vaccine to placebo recipients, 12:4): priming with a lower-dose SeV-Gag given intranasally, followed by boosting with an adenovirus 35-vectored vaccine encoding HIV-1 Gag, reverse transcriptase, integrase, and Nef (Ad35-GRIN) given intramuscularly (SLA); priming with a higher-dose SeV-Gag given intranasally, followed by boosting with Ad35-GRIN given intramuscularly (SHA); priming with Ad35-GRIN given intramuscularly, followed by boosting with a higher-dose SeV-Gag given intranasally (ASH); and priming and boosting with a higher-dose SeV-Gag given intranasally (SHSH)...
January 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28076589/time-volunteered-on-community-health-activities-by-brigadistas-in-nicaragua
#10
Meredith Adamo, Dixmer Rivera, Rashed Shah, Jeanne Koepsell, Leonel Martínez, Justo Pastor Ortiz, Carlos José Jarquín Navarrete, David R Marsh
Objective: To report on how brigadistas ("health brigadiers") in Nicaragua volunteer their time before the introduction of expanded responsibilities (beyond the scope of integrated community case management (iCCM)) for sick children 2-59 months old. Methods: Three complete teams of brigadistas (n = 12 brigadistas total) were selected from remote communities in the department of Matagalpa. Each respondent brigadista was interviewed privately regarding the frequency and duration (i...
November 2016: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28076421/oscillatory-dynamics-supporting-semantic-cognition-meg-evidence-for-the-contribution-of-the-anterior-temporal-lobe-hub-and-modality-specific-spokes
#11
Giovanna Mollo, Piers L Cornelissen, Rebecca E Millman, Andrew W Ellis, Elizabeth Jefferies
The "hub and spoke model" of semantic representation suggests that the multimodal features of objects are drawn together by an anterior temporal lobe (ATL) "hub", while modality-specific "spokes" capture perceptual/action features. However, relatively little is known about how these components are recruited through time to support object identification. We used magnetoencephalography to measure neural oscillations within left ATL, lateral fusiform cortex (FC) and central sulcus (CS) during word-picture matching at different levels of specificity (employing superordinate vs...
2017: PloS One
https://www.readbyqxmd.com/read/28073756/feature-selection-using-a-one-dimensional-na%C3%A3-ve-bayes-classifier-increases-the-accuracy-of-support-vector-machine-classification-of-cdr3-repertoires
#12
Mattia Cinelli, Yuxin Sun, Katharine Best, James M Heather, Shlomit Reich-Zeliger, Eric Shifrut, Nir Friedman, John Shawe-Taylor, Benny Chain
MOTIVATION: Somatic DNA recombination, the hallmark of vertebrate adaptive immunity, has the potential to generate a vast diversity of antigen receptor sequences. How this diversity captures antigen specificity remains incompletely understood. In this study we use high throughput sequencing to compare the global changes in T cell receptor β chain complementarity determining region 3 (CDR3β) sequences following immunization with ovalbumin administered with complete Freund's adjuvant (CFA) or CFA alone...
January 10, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28072839/assessment-of-attention-deficits-in-adolescent-offspring-exposed-to-maternal-type-1-diabetes
#13
Birgitte Bytoft, Sine Knorr, Zuzana Vlachova, Rikke B Jensen, Elisabeth R Mathiesen, Henning Beck-Nielsen, Claus H Gravholt, Dorte M Jensen, Tine D Clausen, Erik L Mortensen, Peter Damm
OBJECTIVE: The aim of this study was to examine the potential association between intrauterine exposure to maternal diabetes and attention deficits in the offspring. RESEARCH DESIGN AND METHODS: Adolescent offspring of a prospectively followed cohort of women with type 1 diabetes (n = 269) and a control group from the background population (n = 293) participated in a follow-up assessment in 2012-2013. We used scores from Conners Continuous Performance Test II to assess attention and based on a principal component analysis we evaluated scores on five different attention factors: focused attention, vigilance, hyperactivity/impulsivity, sustained attention and response style...
2017: PloS One
https://www.readbyqxmd.com/read/28072706/a-randomized-multicenter-phase-iii-study-of-gemcitabine-combined-with-capecitabine-versus-gemcitabine-alone-as-first-line-chemotherapy-for-advanced-pancreatic-cancer-in-south-korea
#14
Hee Seung Lee, Moon Jae Chung, Jeong Youp Park, Seungmin Bang, Seung Woo Park, Ho Gak Kim, Myung Hwan Noh, Sang Hyub Lee, Yong-Tae Kim, Hyo Jung Kim, Chang Duck Kim, Dong Ki Lee, Kwang Bum Cho, Chang Min Cho, Jong Ho Moon, Dong Uk Kim, Dae Hwan Kang, Young Koog Cheon, Ho Soon Choi, Tae Hyeon Kim, Jae Kwang Kim, Jieun Moon, Hye Jung Shin, Si Young Song
BACKGROUND: This phase III trial compared the efficacy and safety of gemcitabine plus capecitabine (GemCap) versus single-agent gemcitabine (Gem) in advanced pancreatic cancer as first-line chemotherapy. METHODS: A total of 214 advanced pancreatic cancer patients were enrolled from 16 hospitals in South Korea between 2007 and 2011. Patients were randomly assigned to receive GemCap (oral capecitabine 1660 mg/m plus Gem 1000 mg/m by 30-minute intravenous infusion weekly for 3 weeks followed by a 1-week break every 4 weeks) or Gem (by 30-minute intravenous infusion weekly for 3 weeks every 4 weeks)...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28072660/two-versus-one-high-frequency-repetitive-transcranial-magnetic-stimulation-session-per-day-for-treatment-resistant-depression-a-randomized-sham-controlled-trial
#15
Christos Theleritis, Pavlos Sakkas, Thomas Paparrigopoulos, Silia Vitoratou, Chara Tzavara, Stefania Bonaccorso, Antonios Politis, Constantin R Soldatos, Costantin Psarros
OBJECTIVES: High-frequency repetitive transcranial magnetic stimulation (HF-rTMS) has proven antidepressant effects, but the optimal frequency of sessions remains unclear. METHODS: We conducted a 3-week, sham-controlled trial to assess the antidepressant efficacy of 1 active HF-rTMS session per day (A1 group) compared with 2 per day (A2 group) and equivalent sham sessions (once a day, S1 group; twice a day, S2 group) in patients with treatment-resistant major depression with a subsequent 2-week follow-up period...
January 9, 2017: Journal of ECT
https://www.readbyqxmd.com/read/28072461/adverse-and-beneficial-effects-in-chinese-hamster-lung-fibroblast-cells-following-radiofrequency-exposure
#16
Anna Sannino, Olga Zeni, Stefania Romeo, Rita Massa, Maria Rosaria Scarfi
In this study, the effect of radiofrequency (RF) exposure to 1950 MHz, Universal Mobile Telecommunication System signal, was investigated in Chinese hamster lung fibroblast cell line (V79). Genotoxic and cytotoxic effects of 20-h exposure at specific absorption rate (SAR) values from 0.15 W/kg to 1.25 W/kg were measured by means of cytokinesis-block micronucleus (MN) assay. Exposure was carried out blinded under strictly controlled conditions of dosimetry and temperature. The effect of RF exposure alone at four SAR values was tested, that is, 0...
January 10, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/28069226/synthesis-of-carbon-loaded-%C3%AE-fe2o3-nanocomposite-and-their-applicability-for-the-selective-removal-of-binary-mixture-of-dyes-by-ultrasonic-adsorption-based-on-response-surface-methodology
#17
Muhammad Saad, Hajira Tahir
The contemporary problems concerning water purification could be resolved by using nanosorbents. The present studies emphasis on the synthesis of γ-Fe2O3-activated carbon nanocomposites (γ-Fe2O3-NP-AC) by sol-gel method. The composition and surface morphology of them were studied by FTIR, EDS, SEM and XRD techniques. Moreover they were employed for the selective removal of binary mixture of dyes including reactive red 223 dye (RR) and Malachite Green dye (MG) by ultrasonic assisted adsorption method. Sonication is the act of applying sound energy to agitate particles in the sample...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28067772/alternating-current-dielectrophoresis-collection-and-chaining-of-phytoplankton-on-chip-comparison-of-individual-species-and-artificial-communities
#18
Coralie Siebman, Orlin D Velev, Vera I Slaveykova
The capability of alternating current (AC) dielectrophoresis (DEP) for on-chip capture and chaining of the three species representative of freshwater phytoplankton was evaluated. The effects of the AC field intensity, frequency and duration on the chaining efficiency and chain lengths of green alga Chlamydomonas reinhardtii, cyanobacterium Synechocystis sp. and diatom Cyclotella meneghiniana were characterized systematically. C. reinhardtii showed an increase of the chaining efficiency from 100 Hz to 500 kHz at all field intensities; C...
January 5, 2017: Biosensors
https://www.readbyqxmd.com/read/28060384/effects-of-functional-endoscopic-sinus-surgery-on-the-acoustics-of-the-sinonasal-tract
#19
M Havel, S Becker, M Schuster, T Johnson, A Maier, J Sundberg
BACKGROUND: Nasal and paranasal cavities are supposed to contribute substantially to the vocal tract resonator properties. However, their acoustical effects as well as the effects of sinus surgery on the voice remain unclear. In this work we investigate resonance phenomena of paranasal sinuses prior to and after various rhinosurgical procedures in cadaveric human sinonasal tracts and corresponding 3D casts. METHODOLOGY: Nasal and paranasal cavities of formalin-preserved cadavers and corresponding 3D replicas were excited by sine-tone sweeps from an earphone placed in the epipharynx...
January 6, 2017: Rhinology
https://www.readbyqxmd.com/read/28060033/the-effects-of-exercise-dose-on-stereotypical-behavior-in-children-with-autism
#20
Stefanie Schmitz Olin, Bridget A McFadden, Devon L Golem, Joseph K Pellegrino, Alan J Walker, David J Sanders, Shawn M Arent
INTRODUCTION: Autism Spectrum Disorder (ASD) is a prevalent neurological disorder in children characterized by restrictive, repetitive patterns of behavior that place an added burden on everyday functions. Aerobic exercise has the propensity to reduce stereotypic behaviors in children with ASD. This study sought to quantify the acute effect of exercise and assess the influence of duration and intensity on frequency of stereotypic behaviors in children with ASD. METHODS: Participants in this study (N=7, Mage=13...
January 4, 2017: Medicine and Science in Sports and Exercise
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