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immunodeficiency obesity

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https://www.readbyqxmd.com/read/29625575/targeted-genome-engineering-in-human-induced-pluripotent-stem-cells-from-patients-with-hemophilia-b-using-the-crispr-cas9-system
#1
Cuicui Lyu, Jun Shen, Rui Wang, Haihui Gu, Jianping Zhang, Feng Xue, Xiaofan Liu, Wei Liu, Rongfeng Fu, Liyan Zhang, Huiyuan Li, Xiaobing Zhang, Tao Cheng, Renchi Yang, Lei Zhang
BACKGROUND: Replacement therapy for hemophilia remains a lifelong treatment. Only gene therapy can cure hemophilia at a fundamental level. The clustered regularly interspaced short palindromic repeats-CRISPR associated nuclease 9 (CRISPR-Cas9) system is a versatile and convenient genome editing tool which can be applied to gene therapy for hemophilia. METHODS: A patient's induced pluripotent stem cells (iPSCs) were generated from their peripheral blood mononuclear cells (PBMNCs) using episomal vectors...
April 6, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29619656/complications-associated-with-underweight-primary-immunodeficiency-patients-prevalence-and-associations-within-the-usidnet-registry
#2
Melanie A Ruffner, Kathleen E Sullivan
PURPOSE: The point prevalence of underweight status and obesity in primary immunodeficiency disease (PID) is unknown, despite the described associations between PID and weight loss and failure to thrive. The goal of this study is to estimate the prevalence of underweight status and obesity in PID patients and to investigate the associations between abnormal body weight and complications of PID. METHODS: Using the US Immunodeficiency Network (USIDNET), we performed a retrospective analysis of 653 pediatric (age 2 to 20 years) and 514 adult (age > 20) patient records with information on patient body mass index (BMI)...
April 4, 2018: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/29553548/intrasplenic-transplantation-of-hepatocytes-after-partial-hepatectomy-in-nod-scid-mice
#3
Barun Das, Jashdeep Bhattacharjee, Preeti, Alaknanda Mishra, Kshama Jain, Srikanth Iyer, Ashwani Kesarwani, Parul Sahu, Prakriti Sinha, Perumal Nagarajan, Pramod Upadhyay
Partial hepatectomy is a versatile and reproducible method to study liver regeneration and the effect of cell based therapeutics in various pathological conditions. Partial hepatectomy also facilitates the increased engraftment and proliferation of transplanted cells by accelerating neovascularization and cell migration towards the liver. Here, we describe a simple protocol for performing 30% hepatectomy and transplantation of cells in the spleen of a non-obese diabetic/severe combined immunodeficient NOD.SCID (NOD...
February 10, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29552124/characterization-of-human-gastric-adenocarcinoma-cell-lines-established-from-peritoneal-ascites
#4
Bożenna Mytar, Małgorzata Stec, Rafał Szatanek, Kazimierz Węglarczyk, Katarzyna Szewczyk, Antoni Szczepanik, Grażyna Drabik, Jarek Baran, Maciej Siedlar, Monika Baj-Krzyworzeka
The three cell lines, designated as gastric cancer (GC)1401, GC1415 and GC1436 were derived from peritoneal effusions from patients with gastric adenocarcinoma. Cell lines were established in tissue culture and in immunodeficient, non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. All cell lines were cultured in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum. These cell lines were grown as an adherent monolayer with doubling time ranging between 25 h (GC1436 cell line) and 30-34 h (GC1401 and GC1415, respectively)...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29519870/leukemia-reconstitution-in-vivo-is-driven-by-cells-in-early-cell-cycle-and-low-metabolic-state
#5
Luca Trentin, Manon Queudeville, Sarah Mirjam Eckhoff, Md Nabiul Hasan, Vera Münch, Elena Boldrin, Felix Seyfried, Stefanie Enzenmüller, Klaus-Michael Debatin, Lüder Hinrich Meyer
In contrast to well-established hierarchical concepts of tumor stem cells, leukemia-initiating cells in B-cell precursor acute lymphoblastic leukemia have not yet been phenotypically identified. Different subpopulations as defined by surface markers have shown equal abilities to reconstitute leukemia upon transplantation onto immunodeficient mice. Using a non-obese diabetes/severe combined immunodeficiency human acute lymphoblastic leukemia mouse model and cell cycle analysis annotating cells to distinct cycle phases, we have functionally characterized leukemia-initiating cells and found that cells in all cell cycle stages are able to reconstitute leukemia in vivo, with early cycling cells (G1blow population) exhibiting the highest leukemia-initiating potential...
March 8, 2018: Haematologica
https://www.readbyqxmd.com/read/29503651/antigen-loading-e-g-glutamic-acid-decarboxylase-65-of-tolerogenic-dcs-toldcs-reduces-their-capacity-to-prevent-diabetes-in-the-non-obese-diabetes-nod-severe-combined-immunodeficiency-model-of-adoptive-cotransfer-of-diabetes-as-well-as-in-nod-mice
#6
David P Funda, Jaroslav Goliáš, Tomáš Hudcovic, Hana Kozáková, Radek Špíšek, Lenka Palová-Jelínková
Tolerogenic DCs (tolDCs) are being researched as a promising intervention strategy also in autoimmune diseases including type 1 diabetes (T1D). T1D is a T-cell-mediated, organ-specific disease with several well-defined and rather specific autoantigens, i.e., proinsulin, insulin, glutamic acid decarboxylase 65 (GAD65), that have been used in animal as well as human intervention trials in attempts to achieve a more efficient, specific immunotherapy. In this study, we have tested tolerogenic DCs for their effectiveness to prevent adoptive transfer of diabetes by diabetogenic splenocytes into non-obese diabetes (NOD)-severe combined immunodeficiency (NOD-SCID) recipients...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29477186/practice-paper-of-the-academy-of-nutrition-and-dietetics-nutrition-intervention-and-human-immunodeficiency-virus-infection
#7
Amanda Willig, Lauri Wright, Theresa A Galvin
Nutrition is an integral component of medical care for people living with human immunodeficiency virus (HIV)/autoimmune deficiency syndrome (AIDS) (PLWHA). The Academy of Nutrition and Dietetics supports integration of medical nutrition therapy into routine care for this population. Fewer PLWHA experience wasting and undernutrition, while the prevalence of obesity and other chronic diseases has increased significantly. Improved understanding of HIV infection's impact on metabolism and chronic inflammation has only increased the complexity of managing chronic HIV infection...
March 2018: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/29406612/2-fluoro-6-methylene-carbocyclic-adenosine-and-its-phosphoramidate-prodrug-a-novel-anti-hbv-agent-active-against-drug-resistant-hbv-mutants
#8
REVIEW
Uma S Singh, Varughese A Mulamoottil, Chung K Chu
Chronic hepatitis B (CHB) is one of the major causes of morbidity and mortality worldwide. Currently, clinically approved nucleos(t)ide analogs (NAs) are very efficient in reducing the load of hepatitis B virus (HBV) with minimum side effects. However, the long-term administration of antiviral drugs promotes HBV for potential drug resistance. To overcome this problem, combination therapies are administered, but HBV progressively altered mutations remain a threat. Therefore, optimally designed NAs are urgently needed to treat drug-resistant HBV...
February 6, 2018: Medicinal Research Reviews
https://www.readbyqxmd.com/read/29393381/feasibility-analysis-of-treating-severe-intrauterine-adhesions-by-transplanting-menstrual-blood-derived-stem-cells
#9
Sheng-Xia Zheng, Jian Wang, Xue-Li Wang, Asim Ali, Li-Min Wu, Yu-Sheng Liu
Intrauterine adhesions (IUA) are associated with the loss of stem cells in the endometrium. Menstrual blood‑derived stem cells (MenSCs) can be isolated from the menstrual blood and differentiated into endometrial cells. To check the transplantation feasibility of MenSCs for the treatment of severe IUA, MenSCs were isolated from menstrual blood, cultured in Dulbecco's modified Eagle's medium (DMEM), identified by immunocytochemistry and flow cytometry, differentiated into endometrial cells in vitro, and finally transplanted into the axillary subcutaneous tissue of non‑obese diabetic/severe combined immunodeficiency (NOD‑SCID) mice to create endometrial tissue...
April 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29371955/a-comparative-global-phosphoproteomics-analysis-of-obinutuzumab-ga101-versus-rituximab-rtx-against-rtx-sensitive-and-resistant-burkitt-lymphoma-bl-demonstrates-differential-phosphorylation-of-signaling-pathway-proteins-after-treatment
#10
Aradhana Awasthi, Delphine C M Rolland, Janet Ayello, Carmella van de Ven, Venkatesha Basrur, Kevin Conlon, Damian Fermin, Matthew J Barth, Christian Klein, Kojo S J Elenitoba-Johnson, Megan S Lim, Mitchell S Cairo
We recently demonstrated that obinutuzumab (GA101), a novel glycoengineered type II CD20 Ab compared to rituximab (RTX) mediates significantly enhanced antibody-dependent cell cytotoxicity (ADCC) in vitro and increased overall survival in a Burkitt lymphoma (BL) xenograft non-obese diabetic severe combined immunodeficiency gamma (NSG) model. In this study we compared the phosphoproteomic changes by pathway analysis following obinutuzumab vs RTX against RTX-sensitive (Raji) and -resistant BL (Raji4RH). Phosphoproteomic analyses were performed by mass-spectrometry (MS)-based label-free quantitative phosphoproteomic profiling...
December 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/29343463/the-impact-of-mhealth-interventions-systematic-review-of-systematic-reviews
#11
REVIEW
Milena Soriano Marcolino, João Antonio Queiroz Oliveira, Marcelo D'Agostino, Antonio Luiz Ribeiro, Maria Beatriz Moreira Alkmim, David Novillo-Ortiz
BACKGROUND: Mobile phone usage has been rapidly increasing worldwide. mHealth could efficiently deliver high-quality health care, but the evidence supporting its current effectiveness is still mixed. OBJECTIVE: We performed a systematic review of systematic reviews to assess the impact or effectiveness of mobile health (mHealth) interventions in different health conditions and in the processes of health care service delivery. METHODS: We used a common search strategy of five major scientific databases, restricting the search by publication date, language, and parameters in methodology and content...
January 17, 2018: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/29313284/endocrinological-aspects-of-hiv-infection
#12
REVIEW
F S Mirza, P Luthra, L Chirch
PURPOSE: Patients with human immunodeficiency virus (HIV) are living longer with effective antiretroviral therapies and are enjoying near normal life span. Therefore, they are encountering endocrine issues faced by the general population along with those specific to HIV infection. The purpose of this article is to review the common endocrine aspects of HIV infection, and the early detection and management strategies for these complications. METHODS: Recent literature on HIV and endocrine disease was reviewed...
January 8, 2018: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29279348/international-union-of-basic-and-clinical-pharmacology-ciii-chemerin-receptors-cmklr1-chemerin-1-and-gpr1-chemerin-2-nomenclature-pharmacology-and-function
#13
Amanda J Kennedy, Anthony P Davenport
Chemerin, a chemoattractant protein and adipokine, has been identified as the endogenous ligand for a G protein-coupled receptor encoded by the gene CMKLR1 (also known as ChemR23), and as a consequence the receptor protein was renamed the chemerin receptor in 2013. Since then, chemerin has been identified as the endogenous ligand for a second G protein-coupled receptor, encoded by the gene GPR1 Therefore, the International Union of Basic and Clinical Pharmacology Committee on Receptor Nomenclature and Drug Classification recommends that the official name of the receptor protein for chemokine-like receptor 1 (CMKLR1) is chemerin receptor 1, and G protein-coupled receptor 1 is chemerin receptor 2 to follow the convention of naming the receptor protein after the endogenous ligand...
January 2018: Pharmacological Reviews
https://www.readbyqxmd.com/read/29225631/initial-intravenous-immunoglobulin-doses-should-be-based-on-adjusted-body-weight-in-obese-patients-with-primary-immunodeficiency-disorders
#14
Rohan Ameratunga
Background: Immunoglobulin therapy plays a critical role in the treatment of immunodeficiency disorders as well as autoimmune and inflammatory conditions. In immunodeficient patients, there has been controversy whether initial loading doses of intravenous (IVIG) should be based on actual body weight or a calculated parameter such as adjusted body weight in obese patients. Case presentation: I describe a patient with Common Variable Immunodeficiency disorder (CVID) who underwent bariatric surgery for morbid obesity...
2017: Allergy, Asthma, and Clinical Immunology
https://www.readbyqxmd.com/read/29181100/kinesin-family-member-11-contributes-to-the-progression-and-prognosis-of-human-breast-cancer
#15
Yuan-Yuan Pei, Gao-Chi Li, Jian Ran, Feng-Xiang Wei
The present study aimed to clarify the association between kinesin family member 11 (KIF11) and human breast cancer, and the effect of KIF11 on breast cancer cell progression. Western blot analysis, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, retroviral infection, immunohistochemistry staining, MTT assay, anchorage-independent growth ability assay and tumorigenicity assay were all used in the present study. Western blot and RT-qPCR analysis revealed that the expression of KIF11 was markedly increased in malignant cells compared with that in non-tumorous cells at the mRNA and protein level...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29159964/blocking-antibodies-induced-by-allergen-specific-immunotherapy-ameliorate-allergic-airway-disease-in-a-human-mouse-chimeric-model
#16
C Vizzardelli, M Gindl, S Roos, C Möbs, B Nagl, F Zimmann, V Sexl, L Kenner, A Neunkirchner, G J Zlabinger, W F Pickl, W Pfützner, B Bohle
BACKGROUND: Allergen-specific immunotherapy (AIT) induces specific blocking antibodies (Ab), which are claimed to prevent IgE-mediated reactions to allergens. Additionally, AIT modulates cellular responses to allergens, for example, by desensitizing effector cells, inducing regulatory T and B lymphocytes and immune deviation. It is still enigmatic which of these mechanisms mediate(s) clinical tolerance. We sought to address the role of AIT-induced blocking Ab separately from cellular responses in a chimeric human/mouse model of respiratory allergy...
November 21, 2017: Allergy
https://www.readbyqxmd.com/read/29127578/comparative-outcomes-of-bariatric-surgery-in-patients-with-and-without-human-immunodeficiency-virus
#17
Gautam Sharma, Andrew T Strong, Mena Boules, Chao Tu, Samuel Szomstein, Raul Rosenthal, John Rodriguez, Alan J Taege, Matthew Kroh
BACKGROUND: Paradoxically, advances in anti-retroviral therapy that has increased survival for patients with human immunodeficiency virus (HIV) have resulted in greater numbers of HIV+ patients developing other chronic diseases, including obesity. Little comparative literature exists detailing perioperative or metabolic outcomes of bariatric surgery in the HIV+ population compared to HIV negative (HIV-) controls. METHODS: This is a retrospective case-control study with both HIV+ (case) and HIV- control patients...
November 10, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29113445/the-noncommunicable-disease-outcomes-of-primary-healthcare-screening-in-two-rural-subdistricts-of-the-eastern-cape-province-south-africa
#18
Paul Rheeder, Angela A Morris-Paxton, Rose-Marie G Ewing, Dillon Woods
BACKGROUND: Middle and lower income countries are challenged with a double burden of disease: while still coping with the onslaught of Human Immunodeficiency Virus (HIV) and increasing levels of tuberculosis (TB), there is a considerable increase in the level of noncommunicable diseases (NCDs). The poor are especially disadvantaged and are at an increased risk for NCDs. Adequate healthcare resources for this environment can only be allocated once the extent and exact nature of the problem is determined...
October 31, 2017: African Journal of Primary Health Care & Family Medicine
https://www.readbyqxmd.com/read/29113169/lentivirus-mediated-rna-interference-inhibits-the-tumorigenicity-of-cluster-of-differentiation-44-tumor-cells-in-hypopharyngeal-cancer
#19
Chenling Shen, Bin Ye, Haixia Hu, Chen Nie, Yan Ma, Jingrong Lv, Hao Wu, Mingliang Xiang
The present study aimed to investigate whether the inhibition of cluster of differentiation (CD)44 expression reduces the tumorigenicity of CD44(+) cancer stem cells in hypopharyngeal cancer. To assess this, effective recombinant CD44 short hairpin RNA-expressing lentiviruses were produced. Lentivirus-mediated RNA interference (RNAi) was then used to knockdown CD44 gene expression in the hypopharyngeal cancer FaDu cell line. The viability of FaDu cells in the two control groups and the RNAi group (RNAi-CD44 lentiviral vector) was detected using an MTT assay in vitro...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29079381/bariatric-surgery-in-hiv-patients-experience-of-an-obesity-reference-center-in-france
#20
Guillaume Pourcher, Gilles Peytavin, Luminita Schneider, Sébastien Gallien, Gilles Force, Valérie Pourcher
BACKGROUND: Few data on bariatric surgery are available regarding obese human immunodeficiency virus (HIV)-infected patients. SETTINGS: Antoine Beclere hospital, Clamart, Paris-sud University, France METHODS: Prospective observational follow-up study recruited HIV-infected patients who underwent bariatric surgery from 2009 to 2015. Baseline demographic characteristics, surgery characteristics, perioperative outcomes, changes in weight loss, HIV markers, antiretroviral drug plasma levels are described...
December 2017: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
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