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electrospun nanofibrous scaffold

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https://www.readbyqxmd.com/read/28415512/study-of-epithelial-differentiation-and-protein-expression-of-keratinocyte-mesenchyme-stem-cell-co-cultivation-on-electrospun-nylon-b-vulgaris-extract-composite-scaffold
#1
Simzar Hosseinzadeh, Masoud Soleimani, Manuchehr Vossoughi, Parviz Ranjbarvan, Shokoh Hamedi, Soheila Zamanlui, Matin Mahmoudifard
Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415483/an-investigation-of-electrospun-henna-leaves-extract-loaded-chitosan-based-nanofibrous-mats-for-skin-tissue-engineering
#2
Iman Yousefi, Mehdi Pakravan, Hoda Rahimi, Abbas Bahador, Zahra Farshadzadeh, Ismael Haririan
Wound healing characteristics of some plant extracts have been well known for many years, and they have been utilized for such applications in traditional way. Recently electrospun nanofibrous mats showed promising properties for tissue engineering and especially for skin repair. It is expected that incorporation of plant extracts into such structures could provide higher performance and synergistic effect for biomedical and wound healing applications. The final purpose of this study is to fabricate chitosan based nanofiber mats loaded with a traditional plant extract of Lawsonia inermis (Henna) leaves to enhance the antibacterial efficacy and wound healing of the precursor nanofibers...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28407447/hierarchical-micro-nanofibrous-bioscaffolds-for-structural-tissue-regeneration
#3
Yun Xu, Wenguo Cui, Yanxia Zhang, Pinghui Zhou, Yong Gu, Xiaofeng Shen, Bin Li, Liang Chen
Various biomimetic scaffolds with hierarchical micro/nanostructures are designed to closely mimic native extracellular matrix network and to guide cell behavior to promote structural tissue generation. However, it remains a challenge to fabricate hierarchical micro/nanoscaled fibrous scaffolds with different functional components that endow the scaffolds with both biochemical and physical features to exert different biological roles during the process of tissue healing. In this study, a biomimetic designed micro/nanoscaled scaffold with integrated hierarchical dual fibrillar components is fabricated in order to repair dura mater and prevent the formation of epidural scars via collagen molecule self-assembly, electrospinning, and biological interface crosslinking strategies...
April 13, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28392386/phosphoprotein-chitosan-electrospun-nanofibrous-scaffold-for-biomineralization
#4
Hongshan Liang, Feng Sheng, Bin Zhou, Yaqiong Pei, Bin Li, Jing Li
In this study, negatively charged phosvitin (PV) and positively charged chitosan (CS) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. Morphologies of the LBL films coating mats were observed by scanning electron microscope (SEM). Afterwards, in vitro biomimetic mineralization was carried out through incubation of the fibrous mats in a simulated body fluid (SBF) solution for different time. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) were used to characterize the morphology and structure of the deposited mineral phase on the scaffolds...
April 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28375819/in-vivo-immune-responses-of-cross-linked-electrospun-tilapia-collagen-membrane
#5
Ammar Mansoor Hassanbhai, Chau Sang Lau, Feng Wen, Praveena Jayaraman, Bee Tin Goh, Na Yu, Swee-Hin Teoh
Collagen has been used extensively in tissue engineering applications. However, the source of collagen has been primarily bovine and porcine. In view of the potential risk of zoonotic diseases and religious constraints associated with bovine and porcine collagen, fish collagen was examined as an alternative. The aim of this study is to use tilapia fish collagen to develop a cross-linked electrospun membrane to be used as a barrier membrane in guided bone regeneration. As there is limited data available on the cytotoxicity and immunogenicity of cross-linked tilapia collagen, in vitro and in vivo tests were performed to evaluate this in comparison to the commercially available Bio-Gide(®) membrane...
March 30, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28371246/long-term-liver-specific-functions-of-hepatocytes-in-electrospun-chitosan-nanofiber-scaffolds-coated-with-fibronectin
#6
Divya Rajendran, Ali Hussain, Derek Yip, Amit Parekh, Anil Shrirao, Cheul H Cho
In this study, a new 3-D liver model was developed using biomimetic nanofiber scaffolds and co-culture system consisting of hepatocytes and fibroblasts for the maintenance of long-term liver functions. The chitosan nanofiber scaffolds were fabricated by the electrospinning technique. To enhance cellular adhesion and spreading, the surfaces of the chitosan scaffolds were coated with fibronectin by adsorption and evaluated for various cell types. Cellular phenotype, protein expression, and liver-specific functions were extensively characterized by immunofluorescent and histochemical stainings, albumin ELISA and Cytochrome p450 detoxification assays, and SEM...
March 28, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28363554/electrospun-alginate-nanofibres-impregnated-with-silver-nanoparticles-preparation-morphology-and-antibacterial-properties
#7
T C Mokhena, A S Luyt
Silver nanoparticles are amongst the most valuable nanoparticles with interesting properties, such as a non-toxic nature and high antibacterial efficiency, making them applicable for tissue scaffold, protective clothing and wound dressing. In this study, silver nanoparticles (AgNPs) have been synthesized using chitosan as reducing and stabilizing agent. The formation of silver nanoparticles was confirmed by UV-vis, and the TEM showed that different shapes were obtained depending on the heating duration. The chitosan/AgNPs was coated onto an electrospun alginate membrane to produce stable polyelectrolyte complex (PEC) nanofibre composites with high antibacterial efficiency...
June 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28341150/in-vitro-and-in-silico-characterization-of-fibrous-scaffolds-comprising-alternate-colistin-sulfate-loaded-and-heat-treated-polyvinyl-alcohol-nanofibrous-sheets
#8
István Sebe, Eszter Ostorházi, Zsolt Bodai, Zsuzsanna Eke, József Szakács, Norbert Krisztián Kovács, Romána Zelkó
A multilayer mat for dispensing colistin sulfate through a body surface was prepared by electrospinning. The fabricated system comprised various polyvinyl alcohol fibrous layers prepared with or without the active ingredient. One of the electrospun layers contained water-soluble colistin sulfate and the other was prepared from the same polymer type and composition without the active drug and was finally heat-treated. The heat treatment modified the supramolecular structure and conferred the polymer nanofibre with the rate-controlling function...
March 21, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28336896/nanofibrous-silver-coated-polymeric-scaffolds-with-tunable-electrical-properties
#9
Adnan Memic, Musab Aldhahri, Ali Tamayol, Pooria Mostafalu, Mohamed Shaaban Abdel-Wahab, Mohamadmahdi Samandari, Kamyar Mollazadeh Moghaddam, Nasim Annabi, Sidi A Bencherif, Ali Khademhosseini
Electrospun micro- and nanofibrous poly(glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) substrates have been extensively used as scaffolds for engineered tissues due to their desirable mechanical properties and their tunable degradability. In this study, we fabricated micro/nanofibrous scaffolds from a PGS-PCL composite using a standard electrospinning approach and then coated them with silver (Ag) using a custom radio frequency (RF) sputtering method. The Ag coating formed an electrically conductive layer around the fibers and decreased the pore size...
March 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28336874/electrospun-nanofibres-containing-antimicrobial-plant-extracts
#10
REVIEW
Wanwei Zhang, Sara Ronca, Elisa Mele
Over the last 10 years great research interest has been directed toward nanofibrous architectures produced by electrospinning bioactive plant extracts. The resulting structures possess antimicrobial, anti-inflammatory, and anti-oxidant activity, which are attractive for biomedical applications and food industry. This review describes the diverse approaches that have been developed to produce electrospun nanofibres that are able to deliver naturally-derived chemical compounds in a controlled way and to prevent their degradation...
February 15, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28330282/in-vitro-cytotoxicity-and-antibacterial-activity-of-silver-coated-electrospun-polycaprolactone-gelatine-nanofibrous-scaffolds
#11
Mim Mim Lim, Naznin Sultana
The development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. In this study, polycaprolactone (PCL) and PCL/gelatine (Ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. Nanofibrous scaffolds were coated with silver (Ag) in different concentrations of silver nitrate (AgNO3) aqueous solution (1.25, 2.5, 5, and 10 %) by using dipping method, drying and followed by ultraviolet (UV) photoreduction...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28324629/electrospun-nerve-guide-scaffold-of-poly-%C3%AE%C2%B5-caprolactone-collagen-nanobioglass-an-in-vitro-study-in-peripheral-nerve-tissue-engineering
#12
Forouzan Mohamadi, Somayeh Ebrahimi-Barough, Mohammad Reza Nourani, Mohammad Ali Derakhshan, Vahabodin Goodarzi, Mohammad Sadegh Nazockdast, Mehdi Farokhi, Roksana Tajerian, Reza Faridi Majidi, Jafar Ai
Among various methods, nerve tissue engineering (NTE) is one of the applicable methods to reconstruct damaged nerve tissues. Electrospinning technique and biomaterials are often considered to fabricate fibrous tissue engineered conduits which have great similarity to the extracellular matrix on fiber structure. Polymer blending is one of the most effective methods for the production of new materials with outstanding features. In this study, conduit structures as main part of the peripheral nerve regeneration based on polymer blend nanocomposites poly(ε-caprolactone)/collagen/nanobioglass (PCL/collagen/NBG) were manufactured by electrospinning technique...
March 21, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28277009/nanofibrous-scaffold-from-electrospinning-biodegradable-waterborne-polyurethane-poly-vinyl-alcohol-for-tissue-engineering-application
#13
Yingke Wu, Weiwei Lin, Hongye Hao, Jiehua Li, Feng Luo, Hong Tan
A series of nanofibrous scaffolds, free of organic solvents, are prepared by electrospinning biodegradable waterborne polyurethane (BWPU) emulsion blending with aqueous poly(vinyl alcohol)(PVA). Tuning the proration of BWPU to PVA, various nanofibers with diameter from 370 to 964 nm are obtained. Strong intermolecular interaction existing between them benefits to the electrospun of BWPU emulsion, which is demonstrated by dynamic thermomechanical analysis and Fourier transform infrared spectroscopy. The nontoxic nanofibrous scaffolds with porous structure, which is similar to the natural extracellular matrix, favor to the attachment and proliferation of the L929 fibroblasts...
May 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28271816/an-overview-on-application-of-natural-substances-incorporated-with-electrospun-nanofibrous-scaffolds-to-development-of-innovative-wound-dressings
#14
Nosratollah Zarghami, Roghayeh Sheervalilou, Amir Fattahi, Abbas Mohajeri, Mehdi Dadashpour, Younes Pilehvar-Soltanahmadi
Conventional dressings are cost-effective and highly absorbent, but not effectual enough to promote hemostasis, adherence and in holding a moist wound bed. Thanks to the developments in the field of nanotechnology and bioengineering, one of the promising current trends is to move progress of innovative wound dressings, merging the application of traditional healing agents and modern products/practices, such as hydrocolloids, hydrogels, films and nanofibers. The electrospun nanofibers webs can provide the essential parameters require for wound dressing to heal wounds including absorptivity, oxygen permeability, and non-adherence to the healing tissue, barrier to bacteria, bioactivity and occlusivity...
March 8, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28269204/electrospinning-of-gelatin-and-smpu-with-carbon-nanotubes-for-tissue-engineering-scaffolds
#15
Monica A Mejia, Lina M Hoyos, Jenniffer Zapata, Luz M Restrepo, Maria E Moneada
The nanofibres created by electrospinning technique are currently used for a variety of applications in tissue engineering; and Gelatin and Polyurethane Shape-Memory (SMPU) have important results in biomedicine. Similarly, carbon nanotubes combined with other biomaterials change important properties, opening new opportunities for biomedical applications. In this work, we constructed scaffold using electrospinning technique based in bovine-hide gelatin, SMPU and both materials hybrid with carbon nanotube. Morphology and cytotoxicity were evaluated and mechanical properties for two materials were obtained in scaffold building...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28256792/the-effect-of-increasing-the-pore-size-of-nanofibrous-scaffolds-on-the-osteogenic-cell-culture-using-a-combination-of-sacrificial-agent-electrospinning-and-ultrasonication
#16
Mahdiyeh Aghajanpoor, Sameereh Hashemi-Najafabadi, Mohamadreza Baghaban-Eslaminejad, Fatemeh Bagheri, Seyyed Mohammad Mousavi, Foruogh Azam Sayyahpour
One of the major problems associated with the electrospun scaffolds is their small pore size, which limits the cellular infiltration for bone tissue engineering. In this study, the effect of increasing the pore size on cellular infiltration was studied in poly/nanohydroxyapatite electrospun scaffolds, which were modified using ultrasonication, co-electrospinning with poly (ethylene oxide), and a combination of both. Ultrasonic process was optimized by central composite design. The ultrasonic output power and time of the process were considered as the effective parameters...
March 3, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28227434/electrospinning-of-gelatin-and-smpu-with-carbon-nanotubes-for-tissue-engineering-scaffolds
#17
Monica A Mejia, Lina M Hoyos, Jenniffer Zapata, Luz M Restrepo, Maria E Moneada, Monica A Mejia, Lina M Hoyos, Jenniffer Zapata, Luz M Restrepo, Maria E Moneada, Lina M Hoyos, Jenniffer Zapata, Monica A Mejia, Luz M Restrepo
The nanofibres created by electrospinning technique are currently used for a variety of applications in tissue engineering; and Gelatin and Polyurethane Shape-Memory (SMPU) have important results in biomedicine. Similarly, carbon nanotubes combined with other biomaterials change important properties, opening new opportunities for biomedical applications. In this work, we constructed scaffold using electrospinning technique based in bovine-hide gelatin, SMPU and both materials hybrid with carbon nanotube. Morphology and cytotoxicity were evaluated and mechanical properties for two materials were obtained in scaffold building...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28221758/synthesis-and-fabrication-of-collagen-coated-ostholamide-electrospun-nanofiber-scaffold-for-wound-healing
#18
Subramani Kandhasamy, Sathiamurthi Perumal, Balaraman Madhan, Narayanan Umamaheswari, Javid Ahmad Banday, Paramasivan Thirumalai Perumal, Vichangal Pridiuldi Santhanakrishnan
A novel scaffold for effective wound healing treatment was developed utilizing natural product bearing collagen-based biocompatible electrospun nanofibers. Initially, ostholamide (OSA) was synthesized from osthole (a natural coumarin), characterized by (1)H, (13)C, DEPT-135 NMR, ESI-MS, and FT-IR spectroscopy analysis. OSA was incorporated into polyhydroxybutyrate (PHB) and gelatin (GEL), which serve as templates for electrospun nanofibers. The coating of OSA-PHB-GEL nanofibers with collagen resulted in PHB-GEL-OSA-COL nanofibrous scaffold which mimics extracellular matrix and serves as an effective biomaterial for tissue engineering applications, especially for wound healing...
March 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28155052/a-nanofibrous-electrospun-patch-to-maintain-human-mesenchymal-cell-stemness
#19
L Pandolfi, N Toledano Furman, Xin Wang, C Lupo, J O Martinez, M Mohamed, F Taraballi, E Tasciotti
Mesenchymal stem cells (MSCs) have been extensively investigated in regenerative medicine because of their crucial role in tissue healing. For these properties, they are widely tested in clinical trials, usually injected in cell suspension or in combination with tridimensional scaffolds. However, scaffolds can largely affect the fates of MSCs, inducing a progressive loss of functionality overtime. The ideal scaffold must delay MSCs differentiation until paracrine signals from the host induce their change. Herein, we proposed a nanostructured electrospun gelatin patch as an appropriate environment where human MSCs (hMSCs) can adhere, proliferate, and maintain their stemness...
March 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28140360/assembling-of-electrospun-meshes-into-three-dimensional-porous-scaffolds-for-bone-repair
#20
Juqing Song, Guanglin Zhu, Lin Wang, Geng An, Xuetao Shi, Yingjun Wang
Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofiber meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the collector, and 3D porous nanofiber scaffolds were fabricated via layer-by-layer assembly with a special binder (18% PCL/DCM solution). The single layer nanofiber mesh possessed very regular morphology with a hollow structure, and the nHA was not only embedded in the nanofiber but also exposed on the surfaces of the fiber, resulting in the improved surface chemical properties...
February 14, 2017: Biofabrication
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