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electrospun nanofibrous scaffold

Jun-Feng Zhou, Yi-Guo Wang, Liang Cheng, Zhao Wu, Xiao-Dan Sun, Jiang Peng
Polypyrrole (PPy) is a biocompatible polymer with good conductivity. Studies combining PPy with electrospinning have been reported; however, the associated decrease in PPy conductivity has not yet been resolved. We embedded PPy into poly(lactic acid) (PLA) nanofibers via electrospinning and fabricated a PLA/PPy nanofibrous scaffold containing 15% PPy with sustained conductivity and aligned topography. There was good biocompatibility between the scaffold and human umbilical cord mesenchymal stem cells as well as Schwann cells...
October 2016: Neural Regeneration Research
Qingqing Yao, Jaqueline G L Cosme, Tao Xu, Jacob M Miszuk, Paulo H S Picciani, Hao Fong, Hongli Sun
Nanofibrous scaffolds that are morphologically/structurally similar to natural ECM are highly interested for tissue engineering; however, the electrospinning technique has the difficulty in directly producing clinically relevant 3D nanofibrous scaffolds with desired structural properties. To address this challenge, we have developed an innovative technique of thermally induced nanofiber self-agglomeration (TISA) recently. The aim of this work was to prepare (via the TISA technique) and evaluate 3D electrospun PCL/PLA blend (mass ratio: 4/1) nanofibrous scaffolds having high porosity of ∼95...
November 15, 2016: Biomaterials
Jialin Song, Binbin Sun, Shen Liu, Wei Chen, Yuanzheng Zhang, Chunyang Wang, Xiumei Mo, Junyi Che, Yuanming Ouyang, Weien Yuan, Cunyi Fan
Electrospinning and electric stimulation (ES) are both promising methods to support neuron adhesion and guide extension of neurons for nerve regeneration. Concurrently, all studies focus on either electrospinning for conduits material or ES in vitro study to accelerate nerve regeneration; few work on the combined use of these two strategies or ES in vivo study. Therefore, this study aimed to investigate the abilities of direct current ES through electrospinning conductive polymer composites composed of polypyrrole and Poly (l-lactic acid-co-ε-caprolactone) (PPY/PLCL) in peripheral nerve regeneration...
2016: Frontiers in Molecular Neuroscience
Nuan Chen, Lingling Tian, Liumin He, Seeram Ramakrishna
Diseases and disorders associated with nervous system such as injuries by trauma and neurodegeneration are shown to be one of the most serious problems in medicine, requiring innovative strategies to trigger and enhance the nerve regeneration. Tissue engineering aims to provide a highly biomimetic environment by using a combination of cells, materials and suitable biological cues, by which the lost body part may be regenerated or even fully rebuilt. Electrospinning, being able to produce extracellular matrix (ECM)-like nanostructures with great flexibility in design and choice of materials, have demonstrated their great potential for fabrication of nerve tissue engineered scaffolds...
September 2016: Neural Regeneration Research
Guorui Jin, Jun Li, Kai Li
Photosensitive semiconducting polymer (SP) combined with light stimulation has shown the capability in promoting the proliferation of human dermal fibroblasts (HDFs). However, the high cytotoxicity of the used SP hindered its further application in bioactive scaffolds. In this contribution, we designed and synthesized a SP, poly (N,N-bis(2-octyldodecyl)-3,6-di(thiophen-2-yl)-2,5-dihydropyrrolo[3,4-c]pyrrole-1,4-dione-alt-thieno[3,2-b]thiophene) (PDBTT) with low cytotoxicity and strong absorbance in red and near-infrared region (600-1200nm)...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Paola Taddei, Silvia Tozzi, Giampaolo Zuccheri, Simona Martinotti, Elia Ranzato, Valeria Chiono, Irene Carmagnola, Masuhiro Tsukada
In this study, composite nanofibrous scaffolds were obtained by electrospinning a trifluoroacetic acid solution containing B. mori silk fibroin (SF) and poly(l-lactic acid) (PLLA) in a 1:1 weight ratio. SF, PLLA and SF/PLLA nanofibres were prepared with average diameter sizes of 360±90nm, 470±240nm and 580±220nm, respectively, as assessed by SEM analysis. Vibrational and thermal analyses showed that upon blending in the SF/PLLA nanofibres, the crystallisation of PLLA was hindered by the presence of SF, which crystallized preferentially and underwent conformational changes that did not significantly change its prevailing β-sheet structure...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Nor Hasrul Akhmal Ngadiman, Noordin Mohd Yusof, Ani Idris, Effaliza Misran, Denni Kurniawan
The use of electrospinning process in fabricating tissue engineering scaffolds has received great attention in recent years due to its simplicity. The nanofibers produced via electrospinning possessed morphological characteristics similar to extracellular matrix of most tissue components. Porosity plays a vital role in developing tissue engineering scaffolds because it influences the biocompatibility performance of the scaffolds. In this study, maghemite (γ-Fe2O3) was mixed with polyvinyl alcohol (PVA) and subsequently electrospun to produce nanofibers...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Luke Burke, Chris J Mortimer, Daniel J Curtis, Aled R Lewis, Rhodri Williams, Karl Hawkins, Thierry G G Maffeis, Chris J Wright
We demonstrate a facile, one-step process to form polymer scaffolds composed of magnetic iron oxide nanoparticles (MNPs) contained within electrospun nano- and micro-fibres of two biocompatible polymers, Poly(ethylene oxide) (PEO) and Poly(vinyl pyrrolidone) (PVP). This was achieved with both needle and free-surface electrospinning systems demonstrating the scalability of the composite fibre manufacture; a 228 fold increase in fibre fabrication was observed for the free-surface system. In all cases the nanoparticle-nanofibre composite scaffolds displayed morphological properties as good as or better than those previously described and fabricated using complex multi-stage techniques...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Xinguo Ye, Sheng Li, Xuanxuan Chen, Yingfei Zhan, Xiaonan Li
Scaffold with good three-dimensional (3D) structure and appropriate surface modification is essential to tissue regeneration in the treatment of tissue or organ failure. Silk fibroin (SF) is a promising scaffolding material with high biocompatibility, cytocompatibility, biodegradability and flexibility. In this study, positively charged polyethylenimine (PEI) and negatively charged SF assembled alternately onto cellulose nanofibrous substrates hydrolyzed from electrospun cellulose acetate nanofibrous mats. The obtained nanofibrous membranes modified with multiple layers of PEI/SF were characterized by field emission scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis...
October 18, 2016: International Journal of Biological Macromolecules
Mamatha M Pillai, J Gopinathan, B Indumathi, Y R Manjoosha, K Santosh Sahanand, B K Dinakar Rai, R Selvakumar, Amitava Bhattacharyya
In this study, silk fibroin nanofibrous scaffolds were developed to investigate the attachment and proliferation of primary human meniscal cells. Silk fibroin (SF)-polyvinyl alcohol (PVA) blended electrospun nanofibrous scaffolds with different blend ratios (2:1, 3:1, and 4:1) were prepared. Morphology of the scaffolds was characterized using atomic force microscopy (AFM). The hybrid nanofibrous mats were crosslinked using 25 % (v/v) glutaraldehyde vapor. In degradation study, the crosslinked nanofiber showed slow degradation of 20 % on weight after 35 days of incubation in simulated body fluid (SBF)...
December 2016: Journal of Membrane Biology
Parisa Sanaei-Rad, Tahereh-Sadat Jafarzadeh Kashi, Ehsan Seyedjafari, Masoud Soleimani
A combination of polymeric materials and bioceramics has recently received a great deal of attention for bone tissue engineering applications. In the present study, hybrid nanofibrous scaffolds were fabricated from PLGA and gelatin via electrospinning and then were coated with hydroxyapatite (HA). They were then characterized and used in stem cell culture studies for the evaluation of their biological behavior and osteogenic differentiation in vitro. This study showed that all PLGA, hybrid PLGA/gelatin and HA-PLGA/gelatin scaffolds were composed of ultrafine fibers with smooth morphology and interconnected pores...
October 5, 2016: Biologicals: Journal of the International Association of Biological Standardization
Xiang Gao, Jinlin Song, Yancong Zhang, Xiao Xu, Siqi Zhang, Ping Ji, Shicheng Wei
The design and development of functional biomimetic systems for programmed stem cell response is a field of topical interest. To mimic bone extracellular matrix, we present an innovative strategy for constructing drug-loaded composite nanofibrous scaffolds in this study, which could integrate multiple cues from calcium phosphate mineral, bioactive molecule, and highly ordered fiber topography for the control of stem cell fate. Briefly, inspired by mussel adhesion mechanism, a polydopamine (pDA)-templated nanohydroxyapatite (tHA) was synthesized and then surface-functionalized with bone morphogenetic protein-7-derived peptides via catechol chemistry...
October 7, 2016: ACS Applied Materials & Interfaces
Farzaneh Khademi, Jafar Ai, Masoud Soleimani, Javad Verdi, Seyed Mohammad Tavangar, Esmaeil Sadroddiny, Mohammad Massumi, Seyed Mahmoud Hashemi
Liver tissue engineering (TE) is rapidly emerging as an effective technique which combines engineering and biological processes to compensate for the shortage of damaged or destroyed liver tissues. We examined the viability, differentiation, and integration of hepatocyte-like cells on an electrospun polyethersulfone (PES) scaffold, derived from human endometrial stem cells (hEnSCs). Natural polymers were separately grafted on plasma-treated PES nanofibers, that is, collagen, heparan sulfate (HS) and collagen-HS...
September 30, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Bum Jin Kim, Hogyun Cheong, Eun-Som Choi, So-Hee Yun, Bong-Hyuk Choi, Ki-Soo Park, Ick Soo Kim, Dae-Hwan Park, Hyung Joon Cha
Nanofibrous scaffolds have been assessed as one of many promising tissue engineering scaffolds to be utilized for wound-healing applications. Previously, we reported multi-functionalized electrospun nanofibrous scaffolds blended with mussel adhesive protein (MAP) and polycaprolactone (PCL), which provide durable mechanical strength, cell-friendly environments, and a substantial ability to capture diverse bioactive molecules without any surface modifications. In the present work, we applied the blended nanofibrous mats of MAP and PCL for in vivo skin wound healing...
January 2017: Journal of Biomedical Materials Research. Part A
Nisha Shankhwar, Manishekhar Kumar, Biman B Mandal, A Srinivasan
Composite nanofibrous membranes based on sol-gel derived 45SiO2 24.5CaO 24.5 Na2O 6 P2O5 (bioglass, BG) and 43SiO2 24.5CaO 24.5 Na2O 6 P2O5 2Fe2O3 (magnetic bioglass, MBG) blended with polyvinyl alcohol (PVA) have been electrospun. These low cost membranes were mostly amorphous in structure with minor crystalline (sodium calcium phosphate) precipitates. All membranes were biodegradable. Among these, the composites exhibited higher tensile strength, better proliferation of human osteosarcoma MG63 cells and higher alkaline phosphatase enzyme activity than the bare PVA membrane, indicating their potential in bone tissue engineering...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Elahe Entekhabi, Masoumeh Haghbin Nazarpak, Fathollah Moztarzadeh, Ali Sadeghi
Given the large differences in nervous tissue and other tissues of the human body and its unique features, such as poor and/or lack of repair, there are many challenges in the repair process of this tissue. Tissue engineering is one of the most effective approaches to repair neural damages. Scaffolds made from electrospun fibers have special potential in cell adhesion, function and cell proliferation. This research attempted to design a high porous nanofibrous scaffold using hyaluronic acid and polycaprolactone to provide ideal conditions for nerve regeneration by applying proper physicochemical and mechanical signals...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Phillip McClellan, William J Landis
Electrospinning has emerged as an effective method of producing nanoscale fibers for use in multiple fields of study. One area of significant interest is nanofiber utilization for tissue engineering because the nanofibrous mats can mimic the native extracellular matrix of biological tissues. A logical next step is the inclusion of certain molecules and compounds to accelerate or increase the efficacy of tissue regeneration. Two methods are under scrutiny for their capability to encapsulate therapeutic compounds within electrospun nanofibers: emulsion and coaxial electrospinning...
2016: BioResearch Open Access
Hyun-Soo Shin, Yun-Min Kook, Hye Jin Hong, Young-Mo Kim, Won-Gun Koh, Jae-Yol Lim
: Development of a tissue-engineered, salivary bio-gland will benefit patients suffering from xerostomia due to loss of fluid-secreting acinar cells. This study was conducted to develop a bioengineering system to induce self-assembly of human parotid epithelial cells (hPECs) cultured on poly ethylene glycol (PEG) hydrogel-micropatterned polycaprolactone (PCL) nanofibrous microwells. Microwells were fabricated by photopatterning of PEG hydrogel in the presence of an electrospun PCL nanofibrous scaffold...
November 2016: Acta Biomaterialia
Mohamad Pezeshki-Modaress, Hamid Mirzadeh, Mojgan Zandi, Sareh Rajabi-Zeleti, Niloofar Sodeifi, Nasser Aghdami, Mohammad R K Mofrad
In this research, fabrication of gelatin/chondroitin sulfate (GAG) nanofibrous scaffolds using electrospiningtechnique for skin tissue engineering was studied. The influence of GAG content on chemical, physical, mechanical and biological properties of the scaffolds were investigated. Human dermal fibroblast (HDF) cells were cultured and bioactivity of electrospun gelatin/GAG scaffolds for skin tissue engineering was assayed. Biological results illustrated that HDF cells attached and spread well on gelatin/GAG nanofibrous scaffolds displaying spindle like shapesand stretching...
September 2, 2016: Journal of Biomedical Materials Research. Part A
Weiming Chen, Shuai Chen, Yosry Morsi, Hany El-Hamshary, Mohamed El-Newhy, Cunyi Fan, Xiumei Mo
Electrospun nanofibers have been used for various biomedical applications. However, electrospinning commonly produces two-dimensional (2D) membranes, which limits the application of nanofibers for the 3D tissue engineering scaffold. In the present study, a porous 3D scaffold (3DS-1) based on electrospun gelatin/PLA nanofibers has been prepared for cartilage tissue regeneration. To further improve the repairing effect of cartilage, a modified scaffold (3DS-2) cross-linked with hyaluronic acid (HA) was also successfully fabricated...
September 21, 2016: ACS Applied Materials & Interfaces
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