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electrospun nanofibrous scaffold

Mamatha M Pillai, J Gopinathan, B Indumathi, Y R Manjoosha, K Santosh Sahanand, B K Dinakar Rai, R Selvakumar, Amitava Bhattacharyya
In this study, silk fibroin nanofibrous scaffolds were developed to investigate the attachment and proliferation of primary human meniscal cells. Silk fibroin (SF)-polyvinyl alcohol (PVA) blended electrospun nanofibrous scaffolds with different blend ratios (2:1, 3:1, and 4:1) were prepared. Morphology of the scaffolds was characterized using atomic force microscopy (AFM). The hybrid nanofibrous mats were crosslinked using 25 % (v/v) glutaraldehyde vapor. In degradation study, the crosslinked nanofiber showed slow degradation of 20 % on weight after 35 days of incubation in simulated body fluid (SBF)...
October 11, 2016: Journal of Membrane Biology
Parisa Sanaei-Rad, Tahereh-Sadat Jafarzadeh Kashi, Ehsan Seyedjafari, Masoud Soleimani
A combination of polymeric materials and bioceramics has recently received a great deal of attention for bone tissue engineering applications. In the present study, hybrid nanofibrous scaffolds were fabricated from PLGA and gelatin via electrospinning and then were coated with hydroxyapatite (HA). They were then characterized and used in stem cell culture studies for the evaluation of their biological behavior and osteogenic differentiation in vitro. This study showed that all PLGA, hybrid PLGA/gelatin and HA-PLGA/gelatin scaffolds were composed of ultrafine fibers with smooth morphology and interconnected pores...
October 5, 2016: Biologicals: Journal of the International Association of Biological Standardization
Xiang Gao, Jinlin Song, Yancong Zhang, Xiao Xu, Siqi Zhang, Ping Ji, Shicheng Wei
The design and development of functional biomimetic systems for programmed stem cell response is a field of topical interest. To mimic bone extracellular matrix, we present an innovative strategy for constructing drug-loaded composite nanofibrous scaffolds in this study, which could integrate multiple cues from calcium phosphate mineral, bioactive molecule, and highly ordered fiber topography for the control of stem cell fate. Briefly, inspired by mussel adhesion mechanism, a polydopamine (pDA)-templated nanohydroxyapatite (tHA) was synthesized and then surface-functionalized with bone morphogenetic protein-7-derived peptides via catechol chemistry...
October 7, 2016: ACS Applied Materials & Interfaces
Farzaneh Khademi, Jafar Ai, Masoud Soleimani, Javad Verdi, Seyed Mohammad Tavangar, Esmaeil Sadroddiny, Mohammad Massumi, Seyed Mahmoud Hashemi
Liver tissue engineering (TE) is rapidly emerging as an effective technique which combines engineering and biological processes to compensate for the shortage of damaged or destroyed liver tissues. We examined the viability, differentiation, and integration of hepatocyte-like cells on an electrospun polyethersulfone (PES) scaffold, derived from human endometrial stem cells (hEnSCs). Natural polymers were separately grafted on plasma-treated PES nanofibers, that is, collagen, heparan sulfate (HS) and collagen-HS...
September 30, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Bum Jin Kim, Hogyun Cheong, Eun-Som Choi, So-Hee Yun, Bong-Hyuk Choi, Ki-Soo Park, Ick Soo Kim, Dae-Hwan Park, Hyung Joon Cha
Nanofibrous scaffolds have been assessed as one of many promising tissue engineering scaffolds to be utilized for wound-healing applications. Previously, we reported multi-functionalized electrospun nanofibrous scaffolds blended with mussel adhesive protein (MAP) and polycaprolactone (PCL), which provide durable mechanical strength, cell-friendly environments, and a substantial ability to capture diverse bioactive molecules without any surface modifications. In the present work, we applied the blended nanofibrous mats of MAP and PCL for in vivo skin wound healing...
September 20, 2016: Journal of Biomedical Materials Research. Part A
Nisha Shankhwar, Manishekhar Kumar, Biman B Mandal, A Srinivasan
Composite nanofibrous membranes based on sol-gel derived 45SiO2 24.5CaO 24.5 Na2O 6 P2O5 (bioglass, BG) and 43SiO2 24.5CaO 24.5 Na2O 6 P2O5 2Fe2O3 (magnetic bioglass, MBG) blended with polyvinyl alcohol (PVA) have been electrospun. These low cost membranes were mostly amorphous in structure with minor crystalline (sodium calcium phosphate) precipitates. All membranes were biodegradable. Among these, the composites exhibited higher tensile strength, better proliferation of human osteosarcoma MG63 cells and higher alkaline phosphatase enzyme activity than the bare PVA membrane, indicating their potential in bone tissue engineering...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Elahe Entekhabi, Masoumeh Haghbin Nazarpak, Fathollah Moztarzadeh, Ali Sadeghi
Given the large differences in nervous tissue and other tissues of the human body and its unique features, such as poor and/or lack of repair, there are many challenges in the repair process of this tissue. Tissue engineering is one of the most effective approaches to repair neural damages. Scaffolds made from electrospun fibers have special potential in cell adhesion, function and cell proliferation. This research attempted to design a high porous nanofibrous scaffold using hyaluronic acid and polycaprolactone to provide ideal conditions for nerve regeneration by applying proper physicochemical and mechanical signals...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Phillip McClellan, William J Landis
Electrospinning has emerged as an effective method of producing nanoscale fibers for use in multiple fields of study. One area of significant interest is nanofiber utilization for tissue engineering because the nanofibrous mats can mimic the native extracellular matrix of biological tissues. A logical next step is the inclusion of certain molecules and compounds to accelerate or increase the efficacy of tissue regeneration. Two methods are under scrutiny for their capability to encapsulate therapeutic compounds within electrospun nanofibers: emulsion and coaxial electrospinning...
2016: BioResearch Open Access
Hyun-Soo Shin, Yun-Min Kook, Hye Jin Hong, Young-Mo Kim, Won-Gun Koh, Jae-Yol Lim
: Development of a tissue-engineered, salivary bio-gland will benefit patients suffering from xerostomia due to loss of fluid-secreting acinar cells. This study was conducted to develop a bioengineering system to induce self-assembly of human parotid epithelial cells (hPECs) cultured on poly ethylene glycol (PEG) hydrogel-micropatterned polycaprolactone (PCL) nanofibrous microwells. Microwells were fabricated by photopatterning of PEG hydrogel in the presence of an electrospun PCL nanofibrous scaffold...
November 2016: Acta Biomaterialia
Mohamad Pezeshki-Modaress, Hamid Mirzadeh, Mojgan Zandi, Sareh Rajabi-Zeleti, Niloofar Sodeifi, Nasser Aghdami, Mohammad R K Mofrad
In this research, fabrication of gelatin/chondroitin sulfate (GAG) nanofibrous scaffolds using electrospiningtechnique for skin tissue engineering was studied. The influence of GAG content on chemical, physical, mechanical and biological properties of the scaffolds were investigated. Human dermal fibroblast (HDF) cells were cultured and bioactivity of electrospun gelatin/GAG scaffolds for skin tissue engineering was assayed. Biological results illustrated that HDF cells attached and spread well on gelatin/GAG nanofibrous scaffolds displaying spindle like shapesand stretching...
September 2, 2016: Journal of Biomedical Materials Research. Part A
Weiming Chen, Shuai Chen, Yosry Morsi, Hany El-Hamshary, Mohamed El-Newhy, Cunyi Fan, Xiumei Mo
Electrospun nanofibers have been used for various biomedical applications. However, electrospinning commonly produces two-dimensional (2D) membranes, which limits the application of nanofibers for the 3D tissue engineering scaffold. In the present study, a porous 3D scaffold (3DS-1) based on electrospun gelatin/PLA nanofibers has been prepared for cartilage tissue regeneration. To further improve the repairing effect of cartilage, a modified scaffold (3DS-2) cross-linked with hyaluronic acid (HA) was also successfully fabricated...
September 21, 2016: ACS Applied Materials & Interfaces
Rupendra Shrestha, Asha Palat, Alan M Punnoose, Shailesh Joshi, D Ponraju, Solomon F D Paul
Electrospun nanofibres have been shown to exhibit extracellular matrix (ECM)-like characteristics required for tissue engineering in terms of porosity, flexibility, fibre organization and strength. This study focuses on developing novel cellulose acetate phthalate (CAP) scaffolds by electrospinning for establishing 3-D chondrocyte and neuronal cultures. Five solvent combinations were employed in fabricating the fibres, namely, acetone/ethanol (9:1), dimethylformamide/tetrahydrofuran/acetone (3:3:4), tetrahydrofuran/acetone (1:1), tetrahydrofuran/ethanol (1:1) and chloroform/methanol (1:1)...
July 29, 2016: Tissue & Cell
K Vocetkova, M Buzgo, V Sovkova, D Bezdekova, P Kneppo, E Amler
OBJECTIVES: Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. METHODS: In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated...
October 2016: Cell Proliferation
Jiang Zhu, Jingjing Luo, Xingyan Zhao, Junjiu Gao, Jie Xiong
In this study, we investigated the phase separation phenomenon of silk fibroin/poly (ɛ-caprolactone) electrospinning solution to improve the performance of silk fibroin/poly (ɛ-caprolactone) electrospun nanofibers. It showed that phase separation does occur in just a few hours in the silk fibroin/poly (ɛ-caprolactone)/formic acid mixture solution. Acetic acid, small molecule nonsolvent for silk fibroin, was first introduced to silk fibroin/poly (ɛ-caprolactone)/formic acid solution, a homogeneous solution without separation for over several days was achieved after mixing for 5 h...
September 2016: Journal of Biomaterials Applications
Daniel Grande, Julien Ramier, Davy Louis Versace, Estelle Renard, Valérie Langlois
Modification of electrospun nanofibrous poly(3-hydroxyalkanoate) (PHA)-based mats was implemented through two routes to obtain biomimetic scaffolds meant for tissue engineering applications. The first strategy relied on a physical functionalization of scaffolds thanks to an original route which combined both electrospinning and electrospraying, while the second approach implied the chemical modification of fiber surface via the introduction of reactive functional groups to further conjugate bioactive molecules...
June 20, 2016: New Biotechnology
Maricela Maldonado, Gerardo Ico, Karen Low, Rebeccah J Luu, Jin Nam
Electrospun scaffolds provide soft nanofibrous networks pliable by human induced pluripotent stem cells. J. Nam and co-workers show on page 1408 that such compliant scaffolding leads to the formation of stem cell colonies with a distinctive three-dimensional morphology. The morphological modulation resulted in the lineage-specific differentiation, suggesting a potential means to enhance translational applications of the stem cells.
June 2016: Advanced Healthcare Materials
Z Rezvani, J R Venugopal, A M Urbanska, D K Mills, S Ramakrishna, M Mozafari
Tissue engineering aims to develop therapeutic products that utilize a combination of scaffolds with viable cell systems or responsive biomolecules derived from such cells, for the repair, restoration/regeneration of tissues. Here, the main goal is to enable body to heal itself by the introduction of electrospun scaffolds, such that the body recognizes as its own and in turn uses it to regenerate "neo-native" functional tissues. During the last decade, innovative nanofibrous scaffolds have attracted substantial interest in bone tissue engineering...
May 28, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
Lingling Tian, Molamma P Prabhakaran, Jue Hu, Menglin Chen, Flemming Besenbacher, Seeram Ramakrishna
Electrospun nanofibrous nerve implants is a promising therapy for peripheral nerve injury, and its performance can be tailored by chemical cues, topographical features as well as electrical properties. In this paper, a surface modified, electrically conductive, aligned nanofibrous scaffold composed of poly (lactic acid) (PLA) and polypyrrole (Ppy), referred to as o-PLAPpy_A, was fabricated for nerve regeneration. The morphology, surface chemistry and hydrophilicity of nanofibers were characterized by Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and water contact angle, respectively...
September 1, 2016: Colloids and Surfaces. B, Biointerfaces
Shivaprasad Manchineella, Greeshma Thrivikraman, Khadija K Khanum, Praveen C Ramamurthy, Bikramjit Basu, T Govindaraju
Skeletal muscle tissue engineering (SMTE) employs designed biomaterial scaffolds for promoting myogenic differentiation of myoblasts to functional myotubes. Oxidative stress plays a significant role in the biocompatibility of biomaterials as well as in the fate of myoblasts during myogenesis and is also associated with pathological conditions such as myotonic dystrophy. The inherent electrical excitability of muscle cells inspired the use of electroactive scaffolds for SMTE. Conducting polymers attracted the attention of researchers for their use in muscle tissue engineering...
May 2016: Advanced Healthcare Materials
Mohammad Mehrasa, Mohammad Ali Asadollahi, Bijan Nasri-Nasrabadi, Kamran Ghaedi, Hossein Salehi, Alireza Dolatshahi-Pirouz, Ayyoob Arpanaei
Poly(lactic-co-glycolic acid) (PLGA) and PLGA/gelatin random nanofibrous scaffolds embedded with different amounts of mesoporous silica nanoparticles (MSNPs) were fabricated using electrospinning method. To evaluate the effects of nanoparticles on the scaffolds, physical, chemical, and mechanical properties as well as in vitro degradation behavior of scaffolds were investigated. The mean diameters of nanofibers were 974±68nm for the pure PLGA scaffolds vs 832±70, 764±80, and 486±64 for the PLGA/gelatin, PLGA/10wt% MSNPs, and the PLGA/gelatin/10wt% MSNPs scaffolds, respectively...
September 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
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