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electrospun nanofibrous scaffold

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https://www.readbyqxmd.com/read/29454157/nanofibrous-plga-electrospun-scaffolds-modified-with-type-i-collagen-influence-hepatocyte-function-and-support-viability-in-vitro
#1
Jessica H Brown, Prativa Das, Michael D DiVito, David Ivancic, Lay Poh Tan, Jason A Wertheim
A major challenge of maintaining primary hepatocytes in vitro is progressive loss of hepatocyte-specific functions, such as protein synthesis and cytochrome P450 (CYP450) catalytic activity. We developed a three-dimensional (3D) nanofibrous scaffold made from poly(L-lactide-co-glycolide) (PLGA) polymer using a newlyoptimizedwet electrospinning techniquethat resulted in a highly porous structure that accommodated inclusion of primary human hepatocytes. Extracellular matrix (ECM) proteins (type I collagen or fibronectin) at varying concentrations were chemically linked to electrospun PLGA using amine coupling to develop an in vitro culture system containing the minimal essential ECM components of the liver micro-environment that preserve hepatocyte function in vitro...
February 14, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29453143/structure-and-physico-mechanical-properties-of-low-temperature-plasma-treated-electrospun-nanofibrous-scaffolds-examined-with-atomic-force-microscopy
#2
Adrian Chlanda, Ewa Kijeńska, Chiara Rinoldi, Michał Tarnowski, Tadeusz Wierzchoń, Wojciech Swieszkowski
Electrospun nanofibrous scaffolds are willingly used in tissue engineering applications due to their tunable mechanical, chemical and physical properties. Additionally, their complex openworked architecture is similar to the native extracellular matrix of living tissue. After implantation such scaffolds should provide sufficient mechanical support for cells. Moreover, it is of crucial importance to ensure sterility and hydrophilicity of the scaffold. For this purpose, a low temperature surface plasma treatment can be applied...
February 8, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29405909/developing-improved-tissue-engineered-buccal-mucosa-grafts-for-urethral-reconstruction
#3
A Simsek, A J Bullock, S Roman, C R Chapple, S Macneil
INTRODUCTION: We aimed to compare alternative synthetic scaffolds suitable for future implantation and to examine the use of an inhibitor of lysyl oxidase (beta-amino- propionitrile [β-APN]) to reduce contraction in these implants. METHODS: Three synthetic scaffolds were compared to natural dermis as substrates for the production of tissue-engineered skin. For natural dermis, Euroskin was used to provide a cell-free cadaveric dermis. Synthetic scaffolds consisted of microfibrous PLA, nanofibrous PHBV, and a micro-/nanofibrous trilayer of PLA-PHBV-PLA...
February 6, 2018: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/29356571/a-biomimetic-emu-oil-blended-electrospun-nanofibrous-mat-for-maintaining-stemness-of-adipose-tissue-derived-stem-cells
#4
Sepideh Jalilzadeh-Tabrizi, Younes Pilehvar-Soltanahmadi, Effat Alizadeh, Shahriar Alipour, Mehdi Dadashpour, Kazem Nejati-Koshki, Nosratollah Zarghami
BACKGROUND: Emu oil (EO) with anti-inflammatory, antioxidative, and wound healing properties can be blended for preparing bioactive nanofibrous scaffold. Adipose tissue-derived stem cells (ADSCs) are promising candidates for tissue engineering, and preserving their stemness potential is vital for further therapeutic applications. AIM: The aim of this study was to fabricate EO-blended nanofiber and investigate its effect on proliferation, survival, and stemness preservation of ADSCs...
January 22, 2018: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/29335199/3-d-mineralized-silk-fibroin-polycaprolactone-composite-scaffold-modified-with-polyglutamate-conjugated-with-bmp-2-peptide-for-bone-tissue-engineering
#5
Jingjing Luo, Haitao Zhang, Jiang Zhu, Xiaokang Cui, Junjiu Gao, Xin Wang, Jie Xiong
In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated with BMP-2 peptide onto silk fibroin (SF)/polycaprolactone (PCL) (SF/PCL) blend nanofibers. The morphology, composition, and mineralisation, were confirmed by FE-SEM, XRD, and FT-IR spectroscopy...
December 28, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29241367/differentiation-of-conjunctiva-mesenchymal-stem-cells-into-secreting-islet-beta-cells-on-plasma-treated-electrospun-nanofibrous-scaffold
#6
Samad Nadri, Ghasem Barati, Hossein Mostafavi, Abdolreza Esmaeilzadeh, Seyed Ehsan Enderami
Transplantation of stem cells using biocompatible nanofibrous scaffolds is a promising therapeutic method for treating Diabetic Mellitus. The aim of this study was to derive insulin-producing cells (IPCs) from conjunctiva-derived mesenchymal stem cell (CJMSCs) and to compare the functionality of differentiated IPCs in a three-dimensional (3D) culture with 2D. Furthermore, the effects of hydrophobicity of scaffold on IPCs differentiation were examined. Scanning electron microscopy (SEM), quantitative real times PCR (qPCR), Immunostaining and flow cytometry were used to analyze fabricated scaffold and the presence of IPCs...
December 14, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29208285/hybrid-nanofibers-based-on-poly-caprolactone-gelatin-hydroxyapatite-nanoparticles-loaded-doxycycline-effective-anti-tumoral-and-antibacterial-activity
#7
Ricardo Ramírez-Agudelo, Karina Scheuermann, Alfonso Gala-García, Ana Paula F Monteiro, Ana Delia Pinzón-García, Maria E Cortés, Rubén D Sinisterra
Cancer is one of the leading causes of morbidity and mortality Worldwide, 19.3 million new cancer cases are expected to be identified in 2025. Among the therapeutic arsenal to cancer control one could find the Doxycycline and the nano hydroxyapatite. The Doxycycline (Dox) not only shown antibiotic effect but also exhibits a wide range of pleiotropic therapeutic properties as the control of the invasive and metastatic cancer cells characteristics. The purpose of the present study was to evaluate both cytotoxicity in vitro and antibacterial activity of electrospun Dox-loaded hybrid nanofibrous scaffolds composed by hydroxyapatite nanoparticles (nHA), poly-ε-caprolactone (PCL) and gelatin (Gel) polymers...
February 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29186202/enhanced-cell-proliferation-and-osteogenic-differentiation-in-electrospun-plga-hydroxyapatite-nanofibre-scaffolds-incorporated-with-graphene-oxide
#8
Chuan Fu, Haotian Bai, Jiaqi Zhu, Zhihao Niu, Yu Wang, Jianan Li, Xiaoyu Yang, Yunshen Bai
One of the goals of bone tissue engineering is to mimic native ECM in architecture and function, creating scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties. The aim of this study was to fabricate nanofibrous matrices by electrospinning a blend of poly (L-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and grapheme oxide (GO) as a favourable platform for bone tissue engineering. The morphology, biocompatibility, mechanical properties, and biological activity of all nanofibrous matrices were compared...
2017: PloS One
https://www.readbyqxmd.com/read/29168916/fabrication-characterization-and-biocompatibility-assessment-of-a-novel-elastomeric-nanofibrous-scaffold-a-potential-scaffold-for-soft-tissue-engineering
#9
Elham Shamirzaei Jeshvaghani, Laleh Ghasemi-Mobarakeh, Reza Mansurnezhad, Fatemeh Ajalloueian, Mahshid Kharaziha, Mohammad Dinari, Maryam Sami Jokandan, Ioannis S Chronakis
With regard to flexibility and strength properties requirements of soft biological tissue, elastomeric materials could be more beneficial in soft tissue engineering applications. The present work investigates the use of an elastic polymer, (polycaprolactone fumarate [PCLF]), for fabricating an electrospun scaffold. PCLF with number-average molecular weight of 13,284 g/mol was synthetized, electrospun PCLF:polycaprolactone (PCL) (70:30) nanofibrous scaffolds were fabricated and a novel strategy (in situ photo-crosslinking along with wet electrospinning) was applied for crosslinking of PCLF in the structure of PCLF:PCL nanofibers was presented...
November 23, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29148704/electrospun-poly-3-hydroxybutyrate-co-4-hydroxybutyrate-graphene-oxide-scaffold-enhanced-properties-and-promoted-in-vivo-bone-repair-in-rats
#10
Tengfei Zhou, Guo Li, Shiyu Lin, Taoran Tian, Quanquan Ma, Qi Zhang, Sirong Shi, Changyue Xue, Wenjuan Ma, Xiaoxiao Cai, Yunfeng Lin
Bone tissue engineering emerges as an advantageous technique to achieve tissue regeneration. Scaffolds for it must present excellent biomechanical properties where bare polymers poorly perform. Development for new biomaterials with high osteogenic capacity is urgently pursued. In this study, an electrospun poly(3-hydroxybutyrate-co-4-hydroxybutyrate/graphene oxide (P34HB/GO) nanofibrous scaffold is successfully fabricated and characterized. The effects of GO amount on scaffold morphology, biomechanical properties, and cellular behaviors are investigated...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29134783/electrospun-titania-fiber-mats-spin-coated-with-thin-polymer-films-as-nanofibrous-scaffolds-for-enhanced-cell-proliferation
#11
Alfin Kurniawan, Chintya Effendi, Meng-Jiy Wang
The incorporation of inorganic materials into electrospun nanofibers has recently gained considerable attention for the development of ECM-like scaffolds with improved mechanical properties and enhanced biological functions for tissue engineering applications. In this study, polymer-inorganic composite fibers consisting of poly(2-ethyl-2-oxazoline) (PEOXA) and tetrabutyl titanate (TBT) as the titanium precursor were successfully fabricated through a combined sol-gel/electrospinning approach. PEOXA/Ti(OR)n composite fibers were obtained with varying amounts of polymer and titanium precursors...
November 14, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29130836/composite-3d-printed-scaffold-with-structured-electrospun-nanofibers-promotes-chondrocyte-adhesion-and-infiltration
#12
M Rampichová, E Košt'áková Kuželová, E Filová, J Chvojka, J Šafka, M Pelcl, J Daňková, E Prosecká, M Buzgo, M Plencner, D Lukáš, E Amler
Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness...
November 13, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/29128593/nanochitosan-enriched-poly-%C3%AE%C2%B5-caprolactone-electrospun-wound-dressing-membranes-a-fine-tuning-of-physicochemical-properties-hemocompatibility-and-curcumin-release-profile
#13
Reshmi Cr, Suja Ps, Manaf O, Sanu Pp, Sujith A
Nanochitosan (NC) enriched Poly (ε-caprolactone) (PCL) nanofibrous membranes were fabricated for controlled curcumin delivery and wound dressing. A significant increase in hydrophilicity, PBS sorption, and vapour transmission were observed by incorporation of NC in PCL. Hemolysis assay, blood clot kinetics and platelet adhesion studies on PCL/NC membrane confirmed the hemocompatibility of the scaffold. NC also imparts appropriate antibacterial activity and biocompatibility promising for a wound dressing material...
November 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29125135/silk-fibroin-amniotic-membrane-3d-bi-layered-artificial-skin
#14
Mazaher Gholipourmalekabadi, A Samadikuchaksaraei, Alexander Marcus Seifalian, Aleksandra Urbanska, Hossein Ghanbarian, John George Hardy, Mir Omrani, Masoud Mozafari, Rui Luis Reis, Subhas Chandra Kundu
Burn injury has been reported to be an important cause of morbidity and mortality and it is still considered as unmet clinical need. Although there is a myriad of effective stem cells suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a 3D bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bilayer...
November 10, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29125131/polymer-scaffolds-for-vascular-tissue-engineering-fabricated-by-combined-electrospinning-and-hot-embossing
#15
Shujie Yan, Xiang Zhang, Liguo Zhang, Hao Liu, Xiaofeng Wang, Qian Li
Although tissue engineering has shown great advances in recent years, creating proper mechanical properties and cell growth microenvironments is still challenging. In this study, electrospun nanofibrous membranes were hot embossed to develop three-dimensional hierarchical micro/nanostructures that load and culture human umbilical-vein endothelial cells (HUVECs). The hot-embossed membranes exhibited not only superior mechanical properties (the tensile strength was 7.01 ± 0.18 MPa and the tensile modulus was 166...
November 10, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29104630/electrospun-collagen-poly-l-lactic-acid-co-%C3%AE%C2%B5-caprolactone-scaffolds-for-conjunctival-tissue-engineering
#16
Qinke Yao, Weijie Zhang, Yang Hu, Junzhao Chen, Chunyi Shao, Xianqun Fan, Yao Fu
Conjunctival injuries are general but intractable ocular surface diseases, the sequelae of which are particularly challenging to treat. A promising therapy for conjunctival injuries is to employ biodegradable scaffolds to deliver conjunctival epithelial cells for repairing damaged or diseased conjunctiva. In the present study, an ultrathin porous nanofibrous scaffold was fabricated by using collagen and poly(L-lactic acid-co-ε-caprolactone) (PLCL) and displayed a thickness of 20 µm, with a high porosity and an average fiber diameter of 248...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29076428/protein-inorganic-nanohybrids-a-potential-symbiosis-in-tissue-engineering
#17
May S Freag, Ahmed O Elzoghby
BACKGROUND: recently, a great interest has been paid to the development of hybrid protein-inorganic nanoparticles (NPs) for tissue engineering applications to combine the merits of both inorganic and protein nanocarriers. OBJECTIVE: this short review primarily discusses the most important advances in the application of the hybrids of proteins (gelatin, zein, silk fibroin,….etc) with inorganic NPs (calcium phosphate NPs, cadmium QDs, carbon nanotubes,…etc) in tissue engineering...
October 27, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/29072193/development-of-poly-1-8-octanediol-co-citrate-and-poly-acrylic-acid-nanofibrous-scaffolds-for-wound-healing-applications
#18
Allison Goins, Vidhya Ramaswamy, Elliott Dirr, Krista Dulany, Sean Irby, Antonio Webb, Josephine Allen
Wound care is one of the leading health care problems in the United States costing billions of dollars yearly. Annually, millions of acute wounds occur due to surgical procedures or traumas such as burns and abrasions, and these wounds can become non-healing due to bacterial infection or underlying pathologies. Current wound care treatments include the use of bioinert constructs combined with topical administration of anti-bacterial agents and growth factors. However, there is a growing need for the development of bioactive wound dressing materials that are able to promote wound healing and the regeneration of healthy tissue...
October 26, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29048241/nanofibrous-electrospun-hdecm-based-hybrid-scaffold-as-wound-dressing-for-reducing-scarring
#19
Tae Hee Kim, Youngmee Jung, Soo Hyun Kim
Produced via electrospinning, poly(l-lactide-co-caprolactone) (PLCL) membranes, which have a porous structure and are biodegradable, are of interest in various medical fields. The porous-structured electrospun membrane is particularly interesting because of several favorable properties: it exudes fluid from the wound, does not build up under the wound covering, and does not cause wound desiccation. Moreover, extracellular matrix (ECM)-based structures derived by tissue decellularization have application as engineered tissue scaffolds and as supports for cellular regeneration...
October 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29043781/direct-writing-electrospinning-of-scaffolds-with-multidimensional-fiber-architecture-for-hierarchical-tissue-engineering
#20
Honglin Chen, Afonso de Botelho Ferreira Braga Malheiro, Clemens van Blitterswijk, Carlos Mota, Paul Andrew Wieringa, Lorenzo Moroni
Nanofibrous structures have long been used as scaffolds for tissue engineering (TE) applications, due to their favorable characteristics, such as high porosity, flexibility, high cell attachment and enhanced proliferation, and overall resemblance to native extracellular matrix (ECM). Such scaffolds can be easily produced at a low cost via electrospinning (ESP), but generally cannot be fabricated with a regular and/or complex geometry, characterized by macropores and uniform thickness. We present here a novel technique for direct writing (DW) with solution ESP to produce complex three-dimensional (3D) multiscale and ultrathin (∼1 μm) fibrous scaffolds with desirable patterns and geometries...
November 8, 2017: ACS Applied Materials & Interfaces
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