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https://www.readbyqxmd.com/read/30007205/conductometric-biosensor-for-arginine-determination-in-pharmaceutics
#1
O V Soldatkina, O O Soldatkin, T P Velychko, V O Prilipko, M A Kuibida, S V Dzyadevych
A new conductometric biosensor based on coimmobilized urease and arginase has been developed for arginine determination in pharmaceutics. First, the main parameters of the selected method of immobilization (concentrations of arginase, urease, and glutaraldehyde, time of incubation) were optimized. An influence of the solution parameters (buffer ionic strength, capacity, pH, Mn2+ concentration) on the biosensor operation was studied, working conditions were optimized. After biosensor optimization, the main analytical characteristics were as follows...
July 5, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/30007169/enhanced-postsynaptic-inhibitory-strength-in-hippocampal-principal-cells-in-high-performing-aged-rats
#2
Trinh Tran, Michela Gallagher, Alfredo Kirkwood
Hyperactivity within the hippocampal formation, frequently observed in aged individuals, is thought to be a potential contributing mechanism to the memory decline often associated with aging. Consequently, we evaluated the postsynaptic strength of excitatory and inhibitory synapses in the granule cells of the dentate gyrus and CA1 pyramidal cells of a rat model of aging, in which each individual was behaviorally characterized as aged impaired (AI) or aged unimpaired (AU, with performance comparable to young (Y) individuals)...
June 12, 2018: Neurobiology of Aging
https://www.readbyqxmd.com/read/30007063/two-plane-assessment-of-knee-muscles-isometric-and-isokinetic-torques-after-anterior-cruciate-ligament-reconstruction
#3
Andrzej Czamara, Aleksandra Królikowska
BACKGROUND We tested the hypothesis that, in patients participating in 17 weeks of postoperative physiotherapy after anterior cruciate ligament reconstruction (ACLR), maximal isometric torque (IT) and peak torque (PT) generated by the muscles affecting the operated knee joint in the sagittal and transverse planes are not restored. We also present the application of IT and PT measurements of the muscles affecting the knee joint in 2 planes of motion. MATERIAL AND METHODS IT and PT of the knee extensor and flexor muscles and the muscles internally rotating the shin were measured in 30 males who participated in postoperative physiotherapy for 17 weeks after ACLR (ACLR group) and 30 males with no injuries (control group)...
July 14, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30006669/exercise-induced-trunk-fatigue-decreases-double-poling-performance-in-well-trained-cross-country-skiers
#4
Elias Bucher, Øyvind Sandbakk, Lars Donath, Ralf Roth, Lukas Zahner, Oliver Faude
PURPOSE: To examine the effects of exercise-induced trunk fatigue on double poling performance, physiological responses and trunk strength in cross-country skiers. METHODS: Sixteen well-trained male cross-country skiers completed two identical pre- and post-performance tests, separated by either a 25-min trunk fatiguing exercise sequence or rest period in a randomized, controlled cross-over design. Performance tests consisted of a maximal trunk flexion and extension test, followed by a 3-min double poling (DP) test on a ski ergometer...
July 13, 2018: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/30006532/magnetostatic-twists-in-room-temperature-skyrmions-explored-by-nitrogen-vacancy-center-spin-texture-reconstruction
#5
Y Dovzhenko, F Casola, S Schlotter, T X Zhou, F Büttner, R L Walsworth, G S D Beach, A Yacoby
Magnetic skyrmions are two-dimensional non-collinear spin textures characterized by an integer topological number. Room-temperature skyrmions were recently found in magnetic multilayer stacks, where their stability was largely attributed to the interfacial Dzyaloshinskii-Moriya interaction. The strength of this interaction and its role in stabilizing the skyrmions is not yet well understood, and imaging of the full spin structure is needed to address this question. Here, we use a nitrogen-vacancy centre in diamond to measure a map of magnetic fields produced by a skyrmion in a magnetic multilayer under ambient conditions...
July 13, 2018: Nature Communications
https://www.readbyqxmd.com/read/30006079/bond-strength-of-pre-coated-flash-free-adhesive-ceramic-brackets-an-in-vitro-comparative-study-on-the-second-mandibular-premolars
#6
Maria-Gabriela Marc, Cédric Bazert, Jean-Pierre Attal
CONTEXT/OBJECTIVES: Clinical studies show a mean bond failure rate of orthodontic brackets, which ranges between 6% and 8%, with a predominance for the second mandibular premolars. The pre-coated flash-free adhesive ceramic brackets allow to obtain, according to their manufacturer, a low bond failure rate, while ensuring a simplified implementation. The aim of this study was to measure the shear bond strength of ceramic brackets bonded with the direct method using a pre-coated flash-free adhesive system, and to compare it with the shear bond strength of the old generation precoated brackets and that of operator coated brackets to the buccal surface of the second mandibular premolars...
July 10, 2018: International Orthodontics
https://www.readbyqxmd.com/read/30005318/atypical-language-characteristics-and-trajectories-in-children-with-22q11-2-deletion-syndrome
#7
Ellen Van Den Heuvel, Eric Manders, Ann Swillen, Inge Zink
BACKGROUND: 22q11.2 deletion syndrome (22q11.2DS) is a genetic condition associated with a highly variable phenotypic expression. During childhood speech and language deficits are commonly observed. Findings of cross-sectional studies suggest syndrome-specific and changing language profiles, but a longitudinal approach to identify developmental changes is still lacking to date. AIMS: The present study aimed to delineate language characteristics and trajectories by comparing the performance of Dutch-speaking school-aged children with 22q11...
June 5, 2018: Journal of Communication Disorders
https://www.readbyqxmd.com/read/30005160/bioinspired-ph-and-temperature-responsive-injectable-adhesive-hydrogels-with-polyplexes-promotes-skin-wound-healing
#8
Thai Minh Duy Le, Huu Thuy Trang Duong, Thavasyappan Thambi, V H Giang Phan, Ji Hoon Jeong, Doo Sung Lee
Despite great potential, the delivery of genetic materials into cells or tissues of interest remains challenging owing to their susceptibility to nuclease degradation, lack of permeability to the cell membrane, and short in vivo half-life, which severely restrict their widespread use in therapeutics. To surmount these shortcomings, we developed a bioinspired in situ-forming pH- and temperature-sensitive injectable hydrogel depot that could control the delivery of DNA-bearing polyplexes for versatile biomedical applications...
July 13, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/30004740/water-compression-gating-of-nanopore-transport
#9
James Wilson, Aleksei Aksimentiev
Electric field-driven motion of biomolecules is a process essential to many analytics methods, in particular, to nanopore sensing, where a transient reduction of nanopore ionic current indicates the passage of a biomolecule through the nanopore. However, before any molecule can be examined by a nanopore, the molecule must first enter the nanopore from the solution. Previously, the rate of capture by a nanopore was found to increase with the strength of the applied electric field. Here, we theoretically show that, in the case of narrow pores in graphene membranes, increasing the strength of the electric field can not only decrease the rate of capture, but also repel biomolecules from the nanopore...
June 29, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/30004319/effects-of-photobiomodulation-on-degranulation-and-number-of-mast-cells-and-wound-strength-in-skin-wound-healing-of-streptozotocin-induced-diabetic-rats
#10
Mohammad Bagheri, Abdollah Amini, Mohammad-Amin Abdollahifar, Seyed Kamran Ghoreishi, Abbas Piryaei, Ramin Pouriran, Sufan Chien, Sara Dadras, Fatemehalsadat Rezaei, Mohammad Bayat
BACKGROUND: A lack of effective treatments still exists for patients suffering from diabetes mellitus. Photobiomodulation is proved as a beneficial therapeutic modality for wounds. OBJECTIVE: The aim of this study is to examine the effect of degranulation of mast cells and total number of mast cells in the remodeling step of an ischemic model of wound healing under the influence of photobiomodulation and conditioned medium (CM) from human bone marrow-derived mesenchymal stem cells (hBM-MSCs-CM), or CM, administered alone and or in combination...
July 6, 2018: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/30004126/how-to-quantify-the-response-to-sexual-selection-on-traits
#11
Jonathan M Henshaw, Michael D Jennions, Loeske E B Kruuk
Natural selection operates via fitness components like mating success, fecundity and longevity, which can be understood as intermediaries in the causal process linking traits to fitness. Sexual selection occurs when traits influence mating or fertilisation success, which, in turn, influences fitness. We show how to quantify both these steps in a single path analysis, leading to better estimates of the strength of sexual selection. Our model controls for confounding variables, such as body size or condition, when estimating the relationship between mating and reproductive success...
July 13, 2018: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/30003681/reducing-seed-waste-and-increasing-value-of-dynamic-intraoperative-implantation-of-pd-103-seeds-in-prostate-brachytherapy
#12
Peter K Taylor, Adam C Riegel
Several nomograms exist for ordering palladium-103 seeds for permanent prostate seed implants (PSI). Excess seeds from PSIs pose additional radiation safety risks and increase the cost of care. This study compared five nomograms to clinical data from dynamic modified-peripheral intraoperative PSI to determine (a) the cause of excess seeds and (b) the optimal nomogram for our institution. Pre- and intraoperative patient data were collected for monotherapy PSIs and compiled into a clinical database. All patients were prescribed 125 Gy with dose coverage of D90% = 100% to the planning target volume (PTV) using 103 Pd seeds with mean air-kerma strength (SK¯) of 2 U...
July 13, 2018: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/30002376/anomalous-mechanical-behavior-of-nanocrystalline-binary-alloys-under-extreme-conditions
#13
S A Turnage, M Rajagopalan, K A Darling, P Garg, C Kale, B G Bazehhour, I Adlakha, B C Hornbuckle, C L Williams, P Peralta, K N Solanki
Fundamentally, material flow stress increases exponentially at deformation rates exceeding, typically, ~103  s-1 , resulting in brittle failure. The origin of such behavior derives from the dislocation motion causing non-Arrhenius deformation at higher strain rates due to drag forces from phonon interactions. Here, we discover that this assumption is prevented from manifesting when microstructural length is stabilized at an extremely fine size (nanoscale regime). This divergent strain-rate-insensitive behavior is attributed to a unique microstructure that alters the average dislocation velocity, and distance traveled, preventing/delaying dislocation interaction with phonons until higher strain rates than observed in known systems; thus enabling constant flow-stress response even at extreme conditions...
July 12, 2018: Nature Communications
https://www.readbyqxmd.com/read/30002268/ultrafast-dynamics-driven-biomolecular-recognition-where-fast-activities-dictate-slow-events
#14
Priya Singh, Damayanti Bagchi, Samir Kumar Pal
In general, biological macromolecules require significant dynamical freedom to carry out their different functions, including signal transduction, metabolism, catalysis and gene regulation. Effectors (ligands, DNA and external milieu, etc) are considered to function in a purely dynamical manner by selectively stabilizing a specific dynamical state, thereby regulating biological function. In particular, proteins in presence of these effectors can exist in several dynamical states with distinct binding or enzymatic activity...
July 2018: Journal of Biosciences
https://www.readbyqxmd.com/read/30001605/effect-of-ionic-strength-and-arginine-on-aggregation-of-uv-irradiated-muscle-glycogen-phosphorylase-b
#15
Tatiana B Eronina, Valeriya V Mikhaylova, Natalia A Chebotareva, Vladimir V Shubin, Boris I Kurganov
In this work the effect of ionic strength and arginine on the kinetics of aggregation of UV-irradiated muscle glycogen phosphorylase b (UV-Phb) was studied using dynamic light scattering at 37 °C at various ionic strengths (0.02-0.7 M). Under these conditions the rate-limiting stage of the overall aggregation process is the structural reorganization of UV-Phb, which can be characterized by the first order rate constant kI . It was shown that an increase in NaCl concentration caused a decrease in the kI value, suggesting a slowdown of the UV-Phb structural reorganization...
July 2, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29999081/shear-induced-parallel-and-transverse-alignments-of-cylinders-in-thin-films-of-diblock-copolymers
#16
Yulong Chen, Qian Xu, Yangfu Jin, Xin Qian, Rui Ma, Jun Liu, Dexin Yang
Coarse-grained Langevin dynamics simulations were performed to investigate the alignment behavior of monolayer films of cylinder-forming diblock copolymers under steady shear, a structure of significant importance for many technical applications such as nanopatterning. The influences of shear conditions, the interactions involved in the films, and the initial morphology of the cylinder-forming phase were examined. Our results showed that above a critical shear rate, the cylinders can align either along the shearing direction or transverse (log-rolling) to the shearing direction depending on the relative strength between the interchain attraction in the cylinders (εAA) and the surface attraction of the confining walls with the film (εBW)...
July 12, 2018: Soft Matter
https://www.readbyqxmd.com/read/29998942/influence-of-a-cylindrical-crosshead-on-shear-bond-testing-of-composite-tooth-interfaces
#17
Chris S Ivanoff, Timothy L Hottel, Franklin Garcia-Godoy
The effect of a cylindrical crosshead on shear bond strength of composite to dentin was assessed by finite element analysis (FEA) after shear bond testing thirty mandibular molars restored with composite cylinders (Tetric EvoCeram-3 mm diameter) perpendicular to conditioned dentin. Cross-sectional geometric models were created with quadrilateral linear plane stress elements. Stress distributions for normal, shear, and major principal stresses were plotted. Mean shear bond strength for the experimental group was 23...
July 12, 2018: Dental Materials Journal
https://www.readbyqxmd.com/read/29998677/-effect-of-cr-%C3%A2-on-coagulation-process-of-different-coagulants
#18
Qian Gao, Da-Wei Zhang, Hui Xu, Jian-Kun Xu, Chong-Miao Zhang, Dong-Sheng Wang
In this study, the effect of Cr(Ⅵ) in industrial wastewater on the coagulation of different aluminum forms under the influence of different particle concentrations was investigated. The coagulation efficiency was determined by examining the removal rate of turbidity, residual aluminum, and residual chromium and by using the residual pH value, zeta potential, and floc properties of the coagulation to reveal the coagulation mechanism of Cr(Ⅵ) in water under different turbidities. The experimental results show that under low turbidity conditions, Cr(Ⅵ) greatly influences the coagulation process of highly polymerized Alb , yet has no obvious effect on oligomeric Ala ...
August 8, 2018: Huan Jing Ke Xue= Huanjing Kexue
https://www.readbyqxmd.com/read/29998573/evaluation-of-compounded-aqueous-milbemycin-oxime-issues-with-formulation-potency-and-reproducibility
#19
Z N Cochrane, D J Berger, A K Viall, D Schrunk, J F Hans Coetzee
OBJECTIVES: To determine the potency and reproducibility of milbemycin oxime when compounded as an aqueous suspension (20 mg/mL). MATERIALS AND METHODS: Preparation choice reflected current prescribing practices. Samples were acquired by prescription from two national veterinary compounding pharmacies at three time points. Two different storage conditions were evaluated and sampled at four time points from the order date (day 7, 14, 21 and 28). Milbemycin oxime recovery was performed by solid-phase extraction and concentration strength measured via high-performance liquid chromatography...
July 11, 2018: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29998443/physical-property-investigation-of-contemporary-glass-ionomer-and-resin-modified-glass-ionomer-restorative-materials
#20
Matthew Moberg, John Brewster, John Nicholson, Howard Roberts
OBJECTIVES: The objective of this study was to investigate selected physical properties of nine contemporary and recently marketed glass ionomer cement (GIC) and four resin-modified glass ionomer cement (RMGI) dental restorative materials. MATERIALS AND METHODS: Specimens (n = 12) were fabricated for fracture toughness and flexure strength using standardized, stainless steel molds. Testing was completed on a universal testing machine until failure. Knoop hardness was obtained using failed fracture toughness specimens on a microhardness tester, while both flexural modulus and flexural toughness was obtained by analysis of the flexure strength results data...
July 11, 2018: Clinical Oral Investigations
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