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Erythrose

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https://www.readbyqxmd.com/read/29080994/regenerative-potential-metabolic-profile-and-genetic-stability-of-brachypodium-distachyon-embryogenic-calli-as-affected-by-successive-subcultures
#1
T C Mamedes-Rodrigues, D S Batista, N M Vieira, E M Matos, D Fernandes, A Nunes-Nesi, C D Cruz, L F Viccini, F T S Nogueira, W C Otoni
Brachypodium distachyon, a model species for forage grasses and cereal crops, has been used in studies seeking improved biomass production and increased crop yield for biofuel production purposes. Somatic embryogenesis (SE) is the morphogenetic pathway that supports in vitro regeneration of such species. However, there are gaps in terms of studies on the metabolic profile and genetic stability along successive subcultures. The physiological variables and the metabolic profile of embryogenic callus (EC) and embryogenic structures (ES) from successive subcultures (30, 60, 90, 120, 150, 180, 210, 240, and 360-day-old subcultures) were analyzed...
October 28, 2017: Protoplasma
https://www.readbyqxmd.com/read/28840775/untargeted-metabolomic-analysis-and-pathway-discovery-in-perinatal-asphyxia-and-hypoxic-ischaemic-encephalopathy
#2
Niamh M Denihan, Jennifer A Kirwan, Brian H Walsh, Warwick B Dunn, David I Broadhurst, Geraldine B Boylan, Deirdre M Murray
Elucidating metabolic effects of hypoxic-ischaemic encephalopathy (HIE) may reveal early biomarkers of injury and new treatment targets. This study uses untargeted metabolomics to examine early metabolic alterations in a carefully defined neonatal population. Infants with perinatal asphyxia who were resuscitated at birth and recovered (PA group), those who developed HIE (HIE group) and healthy controls were all recruited at birth. Metabolomic analysis of cord blood was performed using direct infusion FT-ICR mass spectrometry...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28764953/effect-of-erythritol-formulation-on-the-mortality-fecundity-and-physiological-excretion-in-drosophila-suzukii
#3
Siew Bee Tang, Jana C Lee, Jin Kyo Jung, Man-Yeon Choi
Previously, we studied various combinations of non-nutritive sugars including erythritol and erythrose having a potentially insecticidal effect on Drosophila suzukii. The study suggested two potential physiological changes causing fly mortality: 1) starvation from the feeding of non-metabolizable erythritol and erythrose; 2) abnormal osmotic pressure increased in the hemolymph with erythritol transported from the midgut. In the present study, sucrose and erythritol were applied to blueberries and effects of these combinations on fly mortality and fecundity were monitored in the lab and greenhouse...
August 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28707464/biosynthesis-of-l-erythrose-by-assembly-of-two-key-enzymes-in-gluconobacter-oxydans
#4
Xingxing Zou, Jinping Lin, Xinlei Mao, Shengyun Zhao, Yuhong Ren
L-erythrose, a rare aldotetrose, possesses various pharmacological activities. However, efficient L-erythrose production is challenging. Currently, L-erythrose is produced by a two-step fermentation process from erythritol. Here, we describe a novel strategy for the production of L-erythrose in Gluconobacter oxydans (G. oxydans) by localizing the assembly of L-ribose isomerase (L-RI) to membrane-bound sorbitol dehydrogenase (SDH) via the protein-peptide interactions of the PDZ domain and PDZ ligand. To demonstrate this self-assembly, green fluorescent protein (GFP) replaced L-RI and its movement to membrane-bound SDH was observed by fluorescence microscopy...
September 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28706879/multiple-substrate-usage-of-coxiella-burnetii-to-feed-a-bipartite-metabolic-network
#5
Ina Häuslein, Franck Cantet, Sarah Reschke, Fan Chen, Matteo Bonazzi, Wolfgang Eisenreich
The human pathogen Coxiella burnetii causes Q-fever and is classified as a category B bio-weapon. Exploiting the development of the axenic growth medium ACCM-2, we have now used (13)C-labeling experiments and isotopolog profiling to investigate the highly diverse metabolic network of C. burnetii. To this aim, C. burnetii RSA 439 NMII was cultured in ACCM-2 containing 5 mM of either [U-(13)C3]serine, [U-(13)C6]glucose, or [U-(13)C3]glycerol until the late-logarithmic phase. GC/MS-based isotopolog profiling of protein-derived amino acids, methanol-soluble polar metabolites, fatty acids, and cell wall components (e...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28693571/characterization-of-erythrose-reductase-from-yarrowia-lipolytica-and-its-influence-on-erythritol-synthesis
#6
Tomasz Janek, Adam Dobrowolski, Anna Biegalska, Aleksandra M Mirończuk
BACKGROUND: Erythritol is a natural sweetener that is used in the food industry. It is produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change the insulin level in the blood, and therefore it can be safely used by diabetics. Previously, it has been shown that erythrose reductase (ER), which catalyzes the final step, plays a crucial role in erythritol synthesis. ER reduces erythrose to erythritol with NAD(P)H as a cofactor. Despite many studies on erythritol synthesis by Yarrowia lipolytica, the enzymes involved in this metabolic pathway have ever been described...
July 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28450968/visible-light-controlled-oxidation-of-glucose-using-titania-supported-silver-photocatalysts
#7
Luigi Da Vià, Carlo Recchi, Thomas E Davies, Nicholas Greeves, Jose A Lopez-Sanchez
The visible-light-mediated photo-catalytic selective valorisation of glucose using TiO2-supported Ag nanoparticles is shown for the first time. The optimisation of the catalyst composition, substrate-to-catalyst ratio and reaction medium proved that a near total suppression of the mineralisation pathway could be achieved with a selectivity to partial oxidation products and small-chain monosaccharides as high as 98 %. The primary products were determined to be gluconic acid, arabinose, erythrose, glyceraldehyde and formic acid...
November 22, 2016: ChemCatChem
https://www.readbyqxmd.com/read/28390875/effect-of-non-nutritive-sugars-to-decrease-the-survivorship-of-spotted-wing-drosophila-drosophila-suzukii
#8
Man-Yeon Choi, Siew Bee Tang, Seung-Joon Ahn, Kaushalya G Amarasekare, Peter Shearer, Jana C Lee
In this study, we investigated the effects of non-nutritive sugars and sugar alcohols on the survivorship of spotted wing drosophila, Drosophila suzukii, and found erythritol and erythrose as potentially insecticidal to the fly. In a dose-dependent study, erythritol and erythrose significantly reduced fly longevity, with 100% mortality with 1, 0.5, 0.1 & 0.05M doses after feeding for 7days. When sucrose and erythritol solutions were provided separately to flies for 7days, there was no effect on survivorship regardless of erythritol concentrations...
April 5, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28258150/tm0416-a-hyperthermophilic-promiscuous-nonphosphorylated-sugar-isomerase-catalyzes-various-c5-and-c6-epimerization-reactions
#9
Sun-Mi Shin, Thinh-Phat Cao, Jin Myung Choi, Seong-Bo Kim, Sang-Jae Lee, Sung Haeng Lee, Dong-Woo Lee
There is currently little information on nonphosphorylated sugar epimerases, which are of potential interest for producing rare sugars. We found a gene (the TM0416 gene) encoding a putative d-tagatose-3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima We overexpressed the TM0416 gene in Escherichia coli and purified the resulting recombinant protein for detailed characterization. Amino acid sequence alignment and a structural similarity search revealed that TM0416 is a putative nonphosphorylated sugar epimerase...
May 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28247186/site-selective-13-c-labeling-of-proteins-using-erythrose
#10
Ulrich Weininger
NMR-spectroscopy enables unique experimental studies on protein dynamics at atomic resolution. In order to obtain a full atom view on protein dynamics, and to study specific local processes like ring-flips, proton-transfer, or tautomerization, one has to perform studies on amino-acid side chains. A key requirement for these studies is site-selective labeling with (13)C and/or (1)H, which is achieved in the most general way by using site-selectively (13)C-enriched glucose (1- and 2-(13)C) as the carbon source in bacterial expression systems...
March 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28196894/extending-enzyme-molecular-recognition-with-an-expanded-amino-acid-alphabet
#11
Claire L Windle, Katie J Simmons, James R Ault, Chi H Trinh, Adam Nelson, Arwen R Pearson, Alan Berry
Natural enzymes are constructed from the 20 proteogenic amino acids, which may then require posttranslational modification or the recruitment of coenzymes or metal ions to achieve catalytic function. Here, we demonstrate that expansion of the alphabet of amino acids can also enable the properties of enzymes to be extended. A chemical mutagenesis strategy allowed a wide range of noncanonical amino acids to be systematically incorporated throughout an active site to alter enzymic substrate specificity. Specifically, 13 different noncanonical side chains were incorporated at 12 different positions within the active site of N-acetylneuraminic acid lyase (NAL), and the resulting chemically modified enzymes were screened for activity with a range of aldehyde substrates...
March 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28045507/linear-free-energy-relationship-analysis-of-transition-state-mimicry-by-3-deoxy-d-arabino-heptulosonate-7-phosphate-dahp-oxime-a-dahp-synthase-inhibitor-and-phosphate-mimic
#12
Naresh Balachandran, Frederick To, Paul J Berti
3-Deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthase catalyzes an aldol-like reaction of phosphoenolpyruvate (PEP) with erythrose 4-phosphate (E4P) to form DAHP in the first step of the shikimate biosynthetic pathway. DAHP oxime, in which an oxime replaces the ketone, is a potent inhibitor, with Ki = 1.5 μM. Linear free energy relationship (LFER) analysis of DAHP oxime inhibition using DAHP synthase mutants revealed an excellent correlation between transition state stabilization and inhibition. The equations of LFER analysis were rederived to formalize the possibility of proportional, rather than equal, changes in the free energies of transition state stabilization and inhibitor binding, in accord with the fact that the majority of LFER analyses in the literature demonstrate nonunity slopes...
January 31, 2017: Biochemistry
https://www.readbyqxmd.com/read/28032997/total-facial-discrimination-of-1-3-dipolar-cycloadditions-in-a-d-erythrose-1-3-dioxane-template-computational-studies-of-a-concerted-mechanism
#13
Cristina E A Sousa, António M P Ribeiro, António Gil Fortes, Nuno M F S A Cerqueira, Maria J Alves
A new d-erythrose 1,3-dioxane derivative was synthesized from d-glucose and found to be a highly stereoselective template as a dipolarophile. Different 1,3-dipoles of allenyl-type were employed, giving different regioselectivities, depending on its nature; the regioselectivity is complete with alkyl azides and phenyldiazomethane, but is inexistence with nitrile oxides. Computational studies were performed to understand the mechanisms of cycloadditions. All the studied cycloadditions were found to be concerted involving small free activation energies and are all exoenergonic...
January 9, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/27987869/minor-polysaccharidic-constituents-from-the-red-seaweed-hypnea-musciformis-appearance-of-a-novel-branched-uronic-acid
#14
Vanina A Cosenza, Diego A Navarro, Carlos A Stortz
Two polysaccharide fractions isolated from Hypnea musciformis after room temperature- and hot water extraction, soluble after KCl precipitation of the more abundant carrageenans, were subfractionated by ion-exchange chromatography eluting with increasing concentrations of NaCl. The lowest NaCl concentration (0.2M) eluted agarans. The dl-hybrids (or mixtures) eluted at intermediate concentrations of NaCl. The d/l-galactose ratio and the sulfate proportion increased with the NaCl concentration. Different types of substitution were present, mainly at C-3 with sulfate, Xyl and methylated Gal stubs, as well as low amounts of 3,6-AnGal...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27965953/metabolic-engineering-of-pseudomonas-putida-kt2440-for-the-production-of-para-hydroxy-benzoic-acid
#15
Shiqin Yu, Manuel R Plan, Gal Winter, Jens O Krömer
para-Hydroxy benzoic acid (PHBA) is the key component for preparing parabens, a common preservatives in food, drugs, and personal care products, as well as high-performance bioplastics such as liquid crystal polymers. Pseudomonas putida KT2440 was engineered to produce PHBA from glucose via the shikimate pathway intermediate chorismate. To obtain the PHBA production strain, chorismate lyase UbiC from Escherichia coli and a feedback resistant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase encoded by gene aroG(D146N) were overexpressed individually and simultaneously...
2016: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/27933795/potent-inhibition-of-3-deoxy-d-arabinoheptulosonate-7-phosphate-dahp-synthase-by-dahp-oxime-a-phosphate-group-mimic
#16
Naresh Balachandran, Maren Heimhalt, Peter Liuni, Frederick To, Derek J Wilson, Murray S Junop, Paul J Berti
3-Deoxy-d-arabinoheptulosonate-7-phosphate (DAHP) synthase catalyzes the first step in the shikimate pathway. It catalyzes an aldol-like reaction of phosphoenolpyruvate (PEP) with erythrose 4-phosphate (E4P) to form DAHP. The kinetic mechanism was rapid equilibrium sequential ordered ter ter, with the essential divalent metal ion, Mn(2+), binding first, followed by PEP and E4P. DAHP oxime, in which an oxime group replaces the keto oxygen, was a potent inhibitor, with Ki = 1.5 ± 0.4 μM, though with residual activity at high inhibitor concentrations...
December 6, 2016: Biochemistry
https://www.readbyqxmd.com/read/27896831/anomerization-reaction-of-bare-and-microhydrated-d-erythrose-via-explicitly-correlated-coupled-cluster-approach-two-water-molecules-are-optimal
#17
Marek Szczepaniak, Jerzy Moc
We present a comprehensive benchmark computational study which has explored a complete path of the anomerization reaction of bare d-erythrose involving a pair of the low-energy α- and β-furanose anomers, the former of which was observed spectroscopically (Cabezas et al., Chem. Commun. 2013, 49, 10826). We find that the ring opening of the α-anomer yields the most stable open-chain tautomer which step is followed by the rotational interconversion of the open-chain rotamers and final ring closing to form the β-anomer...
February 15, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/27754504/dietary-fibers-influence-the-intestinal-scfas-and-plasma-metabolites-profiling-in-growing-pigs
#18
W Wu, J Xie, H Zhang
Dietary fibers (DFs), widely used as food additives to replace fat, can benefit metabolic disorders. Soluble and highly fermentable inulin (INU) and insoluble and less fermentable microcrystalline cellulose (MCC) have hypolipidemic or cholesterol-lowering effects, respectively. The current study was aimed at identifying plasma metabolite signatures and metabolic pathways associated with inclusion of DFs with different physiochemical properties, which will lead to a better understanding of functional pathways for DFs to improve metabolic health...
November 9, 2016: Food & Function
https://www.readbyqxmd.com/read/27308197/organocatalytic-synthesis-of-higher-carbon-sugars-efficient-protocol-for-the-synthesis-of-natural-sedoheptulose-and-d-glycero-l-galacto-oct-2-ulose
#19
Oskar Popik, Monika Pasternak-Suder, Sebastian Baś, Jacek Mlynarski
Herein we report a short and efficient protocol for the synthesis of naturally occurring higher-carbon sugars-sedoheptulose (d-altro-hept-2-ulose) and d-glycero-l-galacto-oct-2-ulose-from readily available sugar aldehydes and dihydroxyacetone (DHA). The key step includes a diastereoselective organocatalytic syn-selective aldol reaction of DHA with d-erythrose and d-xylose, respectively. The methodology presented can be expanded to the synthesis of various higher sugars by means of syn-selective carbon-carbon-bond-forming aldol reactions promoted by primary-based organocatalysts...
December 2015: ChemistryOpen
https://www.readbyqxmd.com/read/27280818/purification-and-some-properties-of-an-erythrose-reductase-from-an-aureobasidium-sp-mutant
#20
H Ishizuka, K Tokuoka, T Sasaki, H Taniguchi
An erythrose reductase was obtained from the cells of an Aureobasidium sp. mutant having high erythritol-producing activity. This enzyme was purified 600-fold over the cell-free extract by ammonium sulfate precipitation, ion exchange chromatography, affinity chromatography, and hydrophobic chromatography. It gave a single band on polyacrylamide gel electrophoresis, and had a molecular weight of 37,000 and an isoelectric point of 4.8. This enzyme had maximum reductive activity at 45°C and pH 6.5. The optimum pH of the oxidative reaction was 9...
January 1992: Bioscience, Biotechnology, and Biochemistry
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