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https://www.readbyqxmd.com/read/27749446/tectonic-descemet-stripping-automated-endothelial-keratoplasty-for-the-management-of-sterile-corneal-perforations-in-decompensated-corneas
#1
Yoav Nahum, Irit Bahar, Massimo Busin
PURPOSE: To report the use of Descemet stripping automated endothelial keratoplasty (DSAEK) for treatment of sterile corneal perforations accompanying endothelial decompensation. METHODS: In this multicenter interventional case series, we reviewed the medical records of all tectonic DSAEK surgeries performed at Villa Serena-Villa Igea private Hospitals (Forlì, Italy) and Rabin Medical Center (Petach Tikva, Israel) between January 2014 and March 2016. RESULTS: Three patients with endothelial decompensation and sterile corneal perforation (n = 2) or impending corneal perforation (n = 1) underwent DSAEK between 2014 and 2015 at Villa Igea Hospital, Forlì, Italy, and Rabin Medical Center, Petach Tikva, Israel...
October 5, 2016: Cornea
https://www.readbyqxmd.com/read/27665215/corneal-epithelial-stem-cells-repopulate-the-donor-area-within-1-year-from-limbus-removal-for-limbal-autograft
#2
Massimo Busin, Claudia Breda, Marina Bertolin, Cristina Bovone, Diego Ponzin, Stefano Ferrari, Vanessa Barbaro, Hossein Mostafa Elbadawy
PURPOSE: To determine whether limbal epithelial stem cells (LESCs) repopulate the site harvested for limbal autograft transplantation (LAT), the expression of LESCs markers was evaluated in bioptic specimens obtained from the donor area 12 months or more after surgery. DESIGN: Interventional case series. PARTICIPANTS: Patients who underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn. METHODS: Corneal limbal explants were obtained from 2 sites, the harvested area and the untouched control area, in the donor eyes of 6 patients who previously underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn...
September 21, 2016: Ophthalmology
https://www.readbyqxmd.com/read/27430150/opportunities-and-challenges-for-the-application-of-microfluidic-technologies-in-point-of-care-veterinary-diagnostics
#3
Valentina Busin, Beth Wells, Maïwenn Kersaudy-Kerhoas, Wenmaio Shu, Stewart T G Burgess
There is a growing need for low-cost, rapid and reliable diagnostic results in veterinary medicine. Point-of-care (POC) tests have tremendous advantages over existing laboratory-based tests, due to their intrinsic low-cost and rapidity. A considerable number of POC tests are presently available, mostly in dipstick or lateral flow formats, allowing cost-effective and decentralised diagnosis of a wide range of infectious diseases and public health related threats. Although, extremely useful, these tests come with some limitations...
July 15, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27214234/unraveling-the-sex-chromosome-heteromorphism-of-the-paradoxical-frog-pseudis-tocantins
#4
Kaleb Pretto Gatto, Carmen Silvia Busin, Luciana Bolsoni Lourenço
The paradoxical frog Pseudis tocantins is the only species in the Hylidae family with known heteromorphic Z and W sex chromosomes. The Z chromosome is metacentric and presents an interstitial nucleolar organizer region (NOR) on the long arm that is adjacent to a pericentromeric heterochromatic band. In contrast, the submetacentric W chromosome carries a pericentromeric NOR on the long arm, which is adjacent to a clearly evident heterochromatic band that is larger than the band found on the Z chromosome and justify the size difference observed between these chromosomes...
2016: PloS One
https://www.readbyqxmd.com/read/27188771/investigation-and-treatment-of-ovine-psoroptic-otoacariasis
#5
James Patrick Crilly, Francesca Nunn, Valentina Busin, Edward J Marr, Amy E Jennings, Stewart T G Burgess, Neil D Sargison
BACKGROUND: Psoroptic otoacariasis has been described worldwide and is caused by a mite morphologically indistinguishable from the sheep scab mite Psoroptes ovis. A single treatment of affected sheep with 200 μg/kg of injectable ivermectin is reported to be curative. CASE REPORT: Psoroptes mites were isolated following treatment with ivermectin, but treatment with moxidectin at 1 mg/kg caused complete cessation of clinical signs. Affected animals were seropositive to Pso o 2 antigen enzyme-linked immunosorbent assay (ELISA) and had serum haptoglobin concentrations that overlapped with those described for field infections of classical sheep scab...
June 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27149535/graft-recipient-collagen-lamellar-axis-discrepancy-is-compatible-with-excellent-visual-acuity-after-descemet-stripping-automated-endothelial-keratoplasty
#6
Yoav Nahum, Alfonso Iovieno, Eugenio Lipari, Luigi Fonatana, Massimo Busin
PURPOSE: To evaluate whether a discrepancy between the orientation of graft and recipient collagen lamellae can significantly limit the visual acuity after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: A multicenter cohort study of eyes that underwent DSAEK for Fuchs endothelial dystrophy or pseudophakic bullous keratopathy at Villa Serena-Villa Igea private Hospitals (Forlì, Italy) and at Santa Maria Nuova public hospital (Reggio Emilia, Italy), which were scheduled for a postoperative examination between July and September 2014...
July 2016: Cornea
https://www.readbyqxmd.com/read/27066719/preloaded-tissues-for-descemet-membrane-endothelial-keratoplasty
#7
Mohit Parekh, Alessandro Ruzza, Stefano Ferrari, Massimo Busin, Diego Ponzin
INTRODUCTION: To determine the feasibility of preloading endothelial tissues for Descemet membrane endothelial keratoplasty (DMEK). DESIGN: Laboratory investigation. METHODS: setting: Institutional. PARTICIPANTS: Twenty human donor corneas unsuitable for transplantation with endothelial cell density in a range of 1600-2700 cells/mm(2). INTERVENTION: The endothelium was punched, stripped (8.5 mm diameter) and manually tri-folded with the endothelial side inward...
June 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27050357/two-cases-of-ultrathin-descemet-stripping-automated-endothelial-keratoplasty-utilizing-a-graft-that-had-undergone-radial-keratotomy
#8
Yoav Nahum, Diego Ponzin, Massimo Busin
This is a report of two cases in which tissue that had undergone radial keratotomy (RK) was utilized for double-pass ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK). Postoperative slit-lamp examination, visual acuity, anterior segment optical coherence tomography, and specular microscopy were available 30 months after surgery. Both corneas from a donor, who had undergone RK several years before his demise, and were otherwise suitable for endothelial keratoplasty were prepared for UT-DSAEK using double-pass dissection using first a 300 mm microkeratome head and then a 130μm microkeratome head (ALTK system, Moria, Antony, France)...
February 2016: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/27047845/analysis-on-the-variation-of-medial-rotation-values-according-to-the-position-of-the-humeral-diaphysis
#9
Alberto Naoki Miyazaki, Marcelo Fregoneze, Pedro Doneux Santos, Luciana Andrade da Silva, Guilherme do Val Sella, Carina Cohen, Taís Stedile Busin Giora, Sergio Luiz Checchia, Fabio Raia, Hélio Pekelman, Raquel Cymrot
OBJECTIVE: To analyze the validity of measurements of medial rotation (MR) of the shoulder, using vertebral levels, according to the variation in the position of the humeral diaphysis, and to test the bi-goniometer as a new measuring instrument. METHODS: 140 shoulders (70 patients) were prospectively evaluated in cases presenting unilateral shoulder MR limitation. The vertebral level was evaluated by means of a visual scale and was correlated with the angle obtained according to the position of the humeral diaphysis, using the bi-goniometer developed with the Department of Mechanical Engineering of Mackenzie University...
July 2012: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/27026955/use-of-cortical-structural-homologous-bone-graft-in-femoral-reconstructive-surgery
#10
Milton Valdomiro Roos, Bruno Dutra Roos, Taís Stedile Busin Giora, Thiago Martins Taglietti
UNLABELLED: To perform a clinical and radiographic assessment of patients undergoing surgical treatment using a cortical structural homologous bone graft for femoral reconstruction following mechanical failure of total hip arthroplasty and periprosthetic fractures. METHODS: A retrospective study was conducted on 27 patients who underwent surgical treatment for femoral reconstruction following mechanical failure of total hip arthroplasty (12 cases) and periprosthetic fractures (15 cases), using a cortical structural homologous bone graft and cemented implants, between June 1999 and February 2008...
September 2010: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/26989960/visual-outcomes-of-repeat-versus-primary-descemet-stripping-automated-endothelial-keratoplasty-a-paired-comparison
#11
Yoav Nahum, Michael Mimouni, Silvana Madi, Massimo Busin
PURPOSE: To compare the results of repeat Descemet stripping automated endothelial keratoplasty (DSAEK) with those of primary DSAEK. MATERIALS AND METHODS: This retrospective study consisted of all consecutive eyes that underwent one or more repeat DSAEK surgeries after primary DSAEK performed at Villa Serena-Villa Igea Private Hospitals (Forlì, Italy) between January 2005 and June 2015. A paired comparison between the outcomes of primary and the second DSAEK was performed...
May 2016: Cornea
https://www.readbyqxmd.com/read/26863499/polarimetric-interferometry-for-assessment-of-corneal-stromal-lamellae-orientation
#12
Alfonso Iovieno, Sonia N Yeung, Yoav Nahum, Joshua Teichman, Eugenio Lipari, Massimo Busin, Luigi Fontana
PURPOSE: To analyze corneal stromal lamellae orientation in normal subjects and patients with various corneal conditions using a polarimetric interferometer. METHODS: Thirty-two healthy control subjects (59 eyes) and 16 patients (22 eyes) with corneal conditions or postkeratoplasty were included in the study. All patients were imaged using the interferometer and slit lamp photography. The interferometer produces an orthogonal cross-like image of stromal lamellae by illuminating the cornea in phase-light polarization angle...
April 2016: Cornea
https://www.readbyqxmd.com/read/26772875/outcomes-of-air-injection-within-2%C3%A2-mm-inside-a-deep-trephination-for-deep-anterior-lamellar-keratoplasty-in-eyes-with-keratoconus
#13
Massimo Busin, Vincenzo Scorcia, Pia Leon, Yoav Nahum
PURPOSE: To evaluate the outcomes of a new technique for deep anterior lamellar keratoplasty (DALK) employing the injection of air up to 2 mm inside a deep trephination (intended within 100 μm from the endothelial surface) obtained with a guarded trephine set by means of anterior segment optical coherence tomography (AS OCT). DESIGN: Retrospective, noncomparative, interventional case series. METHODS: The success rate and learning curve of pneumatic dissection in one clinical practice were analyzed in nonscarred keratoconic eyes undergoing a standardized DALK including 9-mm trephination intended to a depth within 100 μm from the endothelial surface, based on the thinnest AS OCT measurement at this site; and injection of air through a cannula advanced 1-2 mm centripetally from the bottom of the trephination...
April 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/26686969/contact-lens-assisted-pull-through-technique-for-delivery-of-tri-folded-endothelium-in-dmek-grafts-minimizes-surgical-time-and-cell-loss
#14
Massimo Busin, Pia Leon, Vincenzo Scorcia, Diego Ponzin
PURPOSE: To evaluate the feasibility and outcomes of contact lens-assisted bimanual pull-through delivery of Descemet membrane endothelial keratoplasty (DMEK) tissue trifolded with the endothelium inward. DESIGN: Prospective, noncomparative, interventional case series. PARTICIPANTS: Forty-two consecutive eyes of 42 patients with Fuchs endothelial dystrophy with or without cataract. INTERVENTION: Standardized DMEK was performed as a single procedure (n = 9) or in combination with phacoemulsification and implantation of a posterior chamber intraocular lens (n = 33) using prestripped donor tissue punched to a diameter of 8...
March 2016: Ophthalmology
https://www.readbyqxmd.com/read/26655486/results-of-descemet-stripping-automated-endothelial-keratoplasty-for-the-treatment-of-late-corneal-decompensation-secondary-to-obstetrical-forceps-trauma
#15
Vincenzo Scorcia, Rocco Pietropaolo, Adriano Carnevali, Valentina De Luca, Andrea Lucisano, Massimo Busin
PURPOSE: To describe the clinical outcomes of Descemet stripping automated endothelial keratoplasty (DSAEK) in a series of patients affected by progressive endothelial cell loss leading to corneal decompensation with Descemet membrane (DM) breaks caused by obstetrical forceps trauma. METHODS: Seven amblyopic eyes of 7 patients affected by unilateral visual loss due to increasing corneal edema were included in the study. In all eyes, slit-lamp examination revealed typical DM breaks and diffuse corneal edema: these findings were compatible with a history of obstetrical forceps-assisted delivery...
March 2016: Cornea
https://www.readbyqxmd.com/read/26538771/a-two-piece-microkeratome-assisted-mushroom-keratoplasty-improves-the-outcomes-and-survival-of-grafts-performed-in-eyes-with-diseased-stroma-and-healthy-endothelium-an-american-ophthalmological-society-thesis
#16
Massimo Busin, Silvana Madi, Vincenzo Scorcia, Paolo Santorum, Yoav Nahum
PURPOSE: To test the hypothesis that a new microkeratome-assisted penetrating keratoplasty (PK) technique employing transplantation of a two-piece mushroom-shaped graft may result in better visual outcomes and graft survival rates than those of conventional PK. METHODS: Retrospective chart review of 96 eyes at low risk and 76 eyes at high risk for immunologic rejection (all with full-thickness central corneal opacity and otherwise healthy endothelium) undergoing mushroom PK between 2004 and 2012 at our Institution...
2015: Transactions of the American Ophthalmological Society
https://www.readbyqxmd.com/read/26356756/postoperative-graft-thickness-obtained-with-single-pass-microkeratome-assisted-ultrathin-descemet-stripping-automated-endothelial-keratoplasty
#17
Yoav Nahum, Pia Leon, Massimo Busin
PURPOSE: To evaluate the results of microkeratome-assisted ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK) using a nomogram developed for the single-pass technique. METHODS: A retrospective study of eyes operated between September 2014 and March 2015 at Villa Serena-Villa Igea Private Hospitals (Forlì, Italy) by a single surgeon (M.B.). Study eyes underwent UT-DSAEK performed using a linear microkeratome-assisted lamellar dissection with disposable heads of different sizes (Moria ALTK system; Moria SA) chosen according to a pachymetry-based nomogram developed at our department...
November 2015: Cornea
https://www.readbyqxmd.com/read/26253099/descemet-stripping-automated-endothelial-keratoplasty-in-fuchs-corneal-endothelial-dystrophy-anterior-segment-optical-coherence-tomography-and-in-vivo-confocal-microscopy-analysis
#18
Rita Mencucci, Eleonora Favuzza, Ruggero Tartaro, Massimo Busin, Gianni Virgili
BACKGROUND: To evaluate the in vivo corneal changes using in vivo confocal microscopy (IVCM) and anterior segment optical coherence tomography (AS-OCT) in patients with Fuchs' dystrophy who underwent Descemet stripping automated endothelial keratoplasty (DSAEK) and the relationship between these changes and the postoperative visual recovery up to 1-year follow-up. METHODS: Before DSAEK and 1 day, 3, 6 and 12 months after surgery 31 patients (39 pseudophakic eyes) underwent a complete ophthalmological evaluation including best corrected visual acuity (BCVA), IVCM (subepithelial haze, interface haze, graft thickness) and AS-OCT (graft thickness)...
2015: BMC Ophthalmology
https://www.readbyqxmd.com/read/26229935/evaluation-of-the-results-from-arthroscopic-surgical-treatment-of-rotator-cuff-injuries-in-patients-aged-65-years-and-over
#19
Alberto Naoki Miyazaki, Luciana Andrade da Silva, Pedro Doenux Santos, Sergio Luiz Checchia, Carina Cohen, Taís Stedile Busin Giora
OBJECTIVES: To evaluate the results from arthroscopic surgical treatment of rotator cuff injuries in patients aged 65 years and over. METHODS: Between 1998 and 2009, 168 patients underwent operations. Five cases were excluded. The remaining 163 patients were stratified according to their age group: 65-69 years (49.1%), 70-74 (26.4%) and 75 years and over (24.5%). Their mean age was 71 years (range: 65-83). There were 63 male patients (38.7%). The mean length of time with pain, from the onset of symptoms to the surgery, was 23 months (range: 2 days to 240 months)...
May 2015: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/26229846/densitometric-study-of-the-clavicle-bone-mineral-density-explains-the-laterality-of-the-fractures
#20
Marcelo Teodoro Ezequiel Guerra, Maria Isabel Pozzi, Gabriela Busin, Lucas Crestana Zanetti, José Antônio Lazzarotto Terra Lopes, Vinícius Orso
INTRODUCTION: Epidemiological studies have shown laterality in clavicle fractures, such that the left side is more frequently fractured. The present study had the aim of evaluating whether the clavicle on the dominant side is denser and thus explaining the greater incidence of fractures on the non-dominant side. MATERIALS AND METHODS: This was a descriptive study on 52 healthy patients, who were classified according to age, sex and whether the dominant or non-dominant side was affected...
September 2014: Revista Brasileira de Ortopedia
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