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https://www.readbyqxmd.com/read/29728540/evaluation-of-cellular-proliferative-activity-in-patients-with-actinic-cheilitis-through-silver-stained-nucleolar-organizer-region-method
#1
Samela Martins, Bernardo Zoehler, Carmen S Busin, Silas Aj de Freitas Filho, Luiz R Paranhos, Maria Ss Linden, Marcos E de Bittencourt, Joao P de Carli
Introduction: Actinic cheilitis (AC) is a lesion potentially malignant that affects the lips after prolonged exposure to solar ultraviolet (UV) radiation. The present study aimed to assess and describe the proliferative cell activity, using silver-stained nucleolar organizer region (AgNOR) quantification proteins, and to investigate the potential associations between AgNORs and the clinical aspects of AC lesions. Materials and methods: Cases diagnosed with AC were selected and reviewed from Center of Histopathological Diagnosis of the Institute of Biological Sciences, Passo Fundo University, Brazil...
April 1, 2018: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/29543665/immunologic-stromal-rejection-after-deep-anterior-lamellar-keratoplasty-with-grafts-of-a-larger-size-9-mm-for-various-stromal-diseases
#2
Giuseppe Giannaccare, Jayne S Weiss, Laura Sapigni, Cristina Bovone, Leila Mattioli, Emilio C Campos, Massimo Busin
PURPOSE: To evaluate the rate and outcomes of immunologic stromal rejection occurring after large deep anterior lamellar keratoplasty (DALK) and the effect of the underlying disease on the cumulative probability of rejection. METHODS: This was a retrospective chart review of all eyes that underwent a 9-mm DALK at Ospedali Privati Forlì (Forlì, Italy). On the basis of preoperative diagnosis, eyes were assigned to group 1: keratoconus, group 2: stromal disease with a low risk for rejection, or group 3: stromal disease with a high risk for rejection...
March 14, 2018: Cornea
https://www.readbyqxmd.com/read/29504956/hypoxia-and-the-prolyl-hydroxylase-inhibitor-fg-4592-protect-corneal-endothelial-cells-from-mechanical-and-perioperative-surgical-stress
#3
Yogesh Bhadange, Jeferson Lautert, Shimin Li, Edward Lawando, Edward T Kim, Mark C Soper, Francis W Price, Marianne O Price, Joseph A Bonanno
PURPOSE: To determine whether hypoxia preconditioning can protect corneal endothelial cells from mechanical stress and perioperative procedures mimicking Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: Preconditioning was delivered by 2 hours of 0.5% oxygen incubation in a hypoxia chamber or by exposure to the prolyl hydroxylase inhibitor FG-4592, which prevents hypoxia-inducible factor-1 alpha degradation. Damage to whole corneas was produced by brief sonication...
April 2018: Cornea
https://www.readbyqxmd.com/read/29375991/descemet-stripping-automated-endothelial-keratoplasty-in-phakic-eyes-incision-modification-reducing-cataract-formation
#4
Jacqueline Beltz, Silvana Madi, Yoav Nahum, Paolo Santorum, Massimo Busin
AIM: To evaluate the efficacy of a technical modification to reduce the incidence of traumatic cataract induced by Descemet stripping automated endothelial keratoplasty (DSAEK) performed in phakic eyes. METHODS: A retrospective cohort study. The records of all patients with a clear crystalline lens and endothelial failure that underwent modified DSAEK at our insitution were reviewed. In this modification, in order to avoid inadvertent touch of the insertion forceps against the exposed crystalline lens while passing across the anterior chamber, the incision sites were shifted from the standard 9 and 3 o'clock positions, superiorly to the 10 and 2 o'clock position respectively...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29374118/treatment-of-sheep-scab-in-the-uk-preventing-the-spread-of-resistant-mites
#5
COMMENT
Valentina Busin
No abstract text is available yet for this article.
January 27, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29339064/factors-associated-with-early-graft-detachment-in-primary-descemet-membrane-endothelial-keratoplasty
#6
Pia Leon, Mohit Parekh, Yoav Nahum, Michael Mimouni, Giuseppe Giannaccare, Laura Sapigni, Alessandro Ruzza, Massimo Busin
PURPOSE: To evaluate the risk factors for early graft detachment in Descemet membrane endothelial keratoplasty (DMEK). DESIGN: Case-control study. METHODS: Participants: A total of 173 donor corneas and 173 eyes of the patients following DMEK or DMEK in combination with phacoemulsification and intraocular lens implantation were included. INTERVENTION: Pre-stripped DMEK grafts were transplanted using pull-through technique...
March 2018: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29275378/results-of-viscobubble-deep-anterior-lamellar-keratoplasty-after-failure-of-pneumatic-dissection
#7
Vincenzo Scorcia, Valentina De Luca, Andrea Lucisano, Adriano Carnevali, Giovanna Carnovale Scalzo, Cristina Bovone, Massimo Busin
AIM: To report the outcomes of bubble formation obtained by means of intrastromal injection of ophthalmic viscoelastic device (OVD) after failure of pneumatic dissection for deep anterior lamellar keratoplasty (DALK). METHODS: DALK was performed in 140 keratoconic eyes of 130 patients by injecting air and OVD only secondarily, after pneumatic dissection had failed; the bubble formation rates after air and OVD injection were recorded; complications, best spectacle-corrected visual acuity (BSCVA) and corneal tomographic parameters were evaluated 3 months, 6 months and 12 months postoperatively, as well as after complete suture removal...
December 23, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28917397/evaluation-of-postoperative-toric-intraocular-lens-alignment-with-anterior-segment-optical-coherence-tomography
#8
Andrea Lucisano, Marco Ferrise, Marco Balestrieri, Massimo Busin, Vincenzo Scorcia
We describe the use of a simple tool to evaluate the postoperative alignment of toric intraocular lenses (IOLs). The entire anterior segment is scanned using anterior segment optical coherence tomography and analyzed with an internal dedicated tool. A topographic map is displayed along with an anterior segment image, including a linear axis marker centered on the corneal apex. The marker can be rotated until it is aligned with the line connecting the IOL marking dots, precisely reproducing the IOL astigmatic axis, which is measured in angle degrees...
August 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28880979/conjunctival-and-limbal-transplantation-from-the-same-living-related-bone-marrow-donor-to-patients-with-severe-ocular-graft-vs-host-disease
#9
Massimo Busin, Giuseppe Giannaccare, Laura Sapigni, Nicoletta Testoni, Pia Leon, Piera Versura, Emilio Campos
No abstract text is available yet for this article.
October 1, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28619155/black-pigmented-anaerobic-bacteria-associated-with-ovine-periodontitis
#10
Ana C Borsanelli, Elerson Gaetti-Jardim, Christiane M Schweitzer, Lorenzo Viora, Valentina Busin, Marcello P Riggio, Iveraldo S Dutra
Periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. This study aimed to detect species of the genera Porphyromonas and Prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). The presence of microorganisms was evaluated by polymerase chain reaction (PCR) using specific primers for Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Porphyromonas gulae, Prevotella buccae, Prevotella intermedia, Prevotella loescheii, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, and Prevotella tannerae...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28489719/microkeratome-assisted-anterior-lamellar-keratoplasty-for-the-correction-of-high-degree-postkeratoplasty-astigmatism
#11
Shay Gutfreund, Pia Leon, Massimo Busin
PURPOSE: To describe the surgical technique and report the outcomes of patients treated with microkeratome-assisted anterior lamellar keratoplasty (MALK) for the correction of high-degree postkeratoplasty astigmatism. METHODS: Four eyes of 2 patients with extremely high (>10 D) and irregular [surface asymmetry index >1.0 D] post-penetrating keratoplasty astigmatism occurring after complete suture removal underwent MALK and were followed for at least 3 years after the procedure...
July 2017: Cornea
https://www.readbyqxmd.com/read/28423859/evidence-for-busines-intelligence-in-health-care-a-literature-review
#12
Liz Loewen, Abdul Roudsari
This paper outlines a systematic literature review undertaken to establish current evidence regarding the impact of Business Intelligence (BI) on health system decision making and organizational performance. The review also examined BI implementation factors contributing to these constructs. Following the systematic review, inductive content analysis was used to categorize themes within the eight articles identified. This study demonstrated there is little evidence based literature focused on BI impact on organizational decision making and performance within health care...
2017: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/28336058/large-9-mm-deep-anterior-lamellar-keratoplasty-with-clearance-of-a-6-mm-optical-zone-optimizes-outcomes-of-keratoconus-surgery
#13
Massimo Busin, Pia Leon, Yoav Nahum, Vincenzo Scorcia
PURPOSE: To evaluate the outcomes of a 9-mm deep anterior lamellar keratoplasty (DALK) with removal of the deep stroma limited to the central 6-mm optical zone. DESIGN: Prospective, noncomparative, interventional case series. PARTICIPANTS: A total of 80 consecutive keratoconic eyes without deep stromal scarring, with at least 1 postoperative examination 1 month after complete suture removal. INTERVENTION: A standardized DALK was performed, including (1) deep trephination of the recipient bed 450 to 550 μm in depth and 9 mm in diameter; (2) pneumatic dissection; (3) debulking of approximately 80% of the anterior stroma; (4) removal of the deep stroma (bubble roof) from a central 6-mm optical zone; and (5) transplantation of a 9-mm anterior corneal lamella cut by microkeratome-assisted dissection (400-μm head) and sutured with a double running 10-0 nylon suture...
July 2017: Ophthalmology
https://www.readbyqxmd.com/read/28129295/asymptomatic-infection-in-decompensated-full-thickness-corneal-grafts-referred-for-repeat-penetrating-keratoplasty
#14
Yoav Nahum, Pia Leon, Benedetta Azzurra Ricci-Filipovic, Davide Camposampiero, Diego Ponzin, Massimo Busin
PURPOSE: We report a case series of asymptomatic infections affecting failed corneal grafts in patients referred for repeat penetrating keratoplasty (PK). METHODS: In this retrospective, noncomparative, interventional case series, we reviewed the medical records of all repeat PK procedures performed at Villa Serena-Villa Igea private Hospitals (Forlì, Italy) between January 2011 and March 2016. Specifically, preoperative and postoperative slit-lamp examinations, and the results of histological and bacteriological examinations, were noted...
April 2017: Cornea
https://www.readbyqxmd.com/read/28079683/factors-associated-with-graft-detachment-after-primary-descemet-stripping-automated-endothelial-keratoplasty
#15
Yoav Nahum, Pia Leon, Michael Mimouni, Massimo Busin
PURPOSE: To identify risk factors associated with postoperative graft detachment after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: In this retrospective institutional cohort study, the study group included all consecutive eyes that underwent primary DSAEK between January 2005 and October 2015 at Villa Serena-Villa Igea private hospitals (Forlì, Italy) and developed postoperative graft detachment (n = 45). The control group included all eyes that underwent primary DSAEK during the same period and did not develop graft detachment (n = 1167)...
March 2017: Cornea
https://www.readbyqxmd.com/read/28017678/deep-anterior-lamellar-keratoplasty-after-descemet-stripping-automated-endothelial-keratoplasty
#16
Shay Gutfreund, Pia Leon, Shmuel Graffi, Massimo Busin
PURPOSE: To report the indications and long-term outcomes of deep anterior lamellar keratoplasty (DALK) performed after Descemet stripping automated endothelial keratoplasty (DSAEK) in cases of visually significant stromal opacities. DESIGN: Retrospective, interventional, consecutive case series. METHODS: Setting: Private hospital. STUDY POPULATION: Thirteen eyes that underwent DALK after DSAEK at our institution. Indications for DALK after DSAEK included both stromal opacities persisting after DSAEK and stromal opacities occurring secondarily in post-DSAEK corneas...
March 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27925102/case-studies-and-role-play-learning-strategies-in-nursing
#17
Ana Luísa Petersen Cogo, Daiane Dal Pai, Graziella Badin Aliti, Heloísa Karnas Hoefel, Karina de Oliveira Azzolin, Lurdes Busin, Margarita Ana Rubin Unicovsky, Maria Henriqueta Luce Kruse
Objective: to report professors' experience in a public university of Southern region of Brazil using case studies and role play as learning strategies for nursing care of hospitalized adults. Method: learning experience report from the Nursing Care of Adults I class of nursing undergraduate course. Results: the development of case studies and role play considered health care needs from epidemiological profile of chronic noncommunicable diseases morbidity and mortality, nursing as an assisting method, and social aspects of hospitalized individuals...
November 2016: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/27749446/tectonic-descemet-stripping-automated-endothelial-keratoplasty-for-the-management-of-sterile-corneal-perforations-in-decompensated-corneas
#18
MULTICENTER STUDY
Yoav Nahum, Irit Bahar, Massimo Busin
PURPOSE: To report the use of Descemet stripping automated endothelial keratoplasty (DSAEK) for treatment of sterile corneal perforations accompanying endothelial decompensation. METHODS: In this multicenter interventional case series, we reviewed the medical records of all tectonic DSAEK surgeries performed at Villa Serena-Villa Igea private Hospitals (Forlì, Italy) and Rabin Medical Center (Petach Tikva, Israel) between January 2014 and March 2016. RESULTS: Three patients with endothelial decompensation and sterile corneal perforation (n = 2) or impending corneal perforation (n = 1) underwent DSAEK between 2014 and 2015 at Villa Igea Hospital, Forlì, Italy, and Rabin Medical Center, Petach Tikva, Israel...
December 2016: Cornea
https://www.readbyqxmd.com/read/27665215/corneal-epithelial-stem-cells-repopulate-the-donor-area-within-1-year-from-limbus-removal-for-limbal-autograft
#19
Massimo Busin, Claudia Breda, Marina Bertolin, Cristina Bovone, Diego Ponzin, Stefano Ferrari, Vanessa Barbaro, Hossein Mostafa Elbadawy
PURPOSE: To determine whether limbal epithelial stem cells (LESCs) repopulate the site harvested for limbal autograft transplantation (LAT), the expression of LESCs markers was evaluated in bioptic specimens obtained from the donor area 12 months or more after surgery. DESIGN: Interventional case series. PARTICIPANTS: Patients who underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn. METHODS: Corneal limbal explants were obtained from 2 sites, the harvested area and the untouched control area, in the donor eyes of 6 patients who previously underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn...
December 2016: Ophthalmology
https://www.readbyqxmd.com/read/27430150/opportunities-and-challenges-for-the-application-of-microfluidic-technologies-in-point-of-care-veterinary-diagnostics
#20
REVIEW
Valentina Busin, Beth Wells, Maïwenn Kersaudy-Kerhoas, Wenmaio Shu, Stewart T G Burgess
There is a growing need for low-cost, rapid and reliable diagnostic results in veterinary medicine. Point-of-care (POC) tests have tremendous advantages over existing laboratory-based tests, due to their intrinsic low-cost and rapidity. A considerable number of POC tests are presently available, mostly in dipstick or lateral flow formats, allowing cost-effective and decentralised diagnosis of a wide range of infectious diseases and public health related threats. Although, extremely useful, these tests come with some limitations...
October 2016: Molecular and Cellular Probes
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