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https://www.readbyqxmd.com/read/28735329/antistaphylococcal-activity-of-selected-thiourea-derivatives
#1
Joanna Stefańska, Karolina Stępień, Anna Bielenica, Małgorzata Wrzosek, Marta Struga
Five of thiourea derivatives were prepared using as a starting compound 3-(trifluoromethyl)aniline, 4-chloro-3-nitroaniline, 1,3-thiazol-2-amine, 2H-1,2,3-triazol-4-amine and commercial isothiocyanates. All compounds were evaluated in vitro for antimicrobial activity. Derivatives 2 and 3 showed the highest inhibition against Gram-positive cocci (standard and hospital strains). The observed MIC values were in the range of 0.5-8 μg/ml. The products effectively inhibited the formation of biofilms of methicillin-resistant and standard strains of Staphylococcus epidermidis...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28733150/seven-new-lignan-glycosides-from-the-branches-of-alangium-kurzii-craib-var-laxifolium
#2
Jie Ren, Yang-Guo Xie, Ying-Ying Huang, Sheng-Lan Zhu, Shi-Kai Yan, Hui-Zi Jin, Wei-Dong Zhang
Seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of Alangium kurzii Craib var. laxifolium. Their structures were established by spectroscopic analysis and circular dichroism (CD) and X-ray analysis. The isolated compounds were evaluated for their antibacterial activities against Staphylococcus aureus CI1011, Streptococcus suis CI1608, Salmonella gallinarum CI0912, Enterococcus faecalis CI1304, Aeromonas hydrophila CI1008, Escherichia coli CI151012, Vibrio parahaemolyticus CI150506, Klebsiella pneumoniae CI131216, Pseudomonas aeruginosa CI1011, Staphylococcus epidermidis CI1110, and Streptococcus agalactiae CI1302...
July 19, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28731488/contamination-risk-of-synovial-biopsy-cultures-in-total-hip-arthroplasty-a-prospective-review-of-100-cases
#3
Christophe Pattyn, Thomas De Bo, Joris Anthonissen, Philippe Willekens, Geert Claeys, Emmanuel A Audenaert
INTRODUCTION: Cultures of deep synovial biopsies remain an important tool in diagnosing periprosthetic joint infection, a devastating complication following total hip arthroplasty (THA). Recent reports of unexpected positive intraoperative cultures in aseptic revision arthroplasty, however, challenge the validity and interpretation of these cultures. The aim of this study was to evaluate the contamination risk of synovial biopsy cultures collected intraoperatively during primary THA of healthy subjects...
July 19, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/28725151/chemical-constituents-of-helichrysum-italicum-roth-g-don-essential-oil-and-their-antimicrobial-activity-against-gram-positive-and-gram-negative-bacteria-filamentous-fungi-and-candida-albicans
#4
Bouzid Djihane, Nouioua Wafa, Soltani Elkhamssa, De Haro Juan Pedro, Angeles Esteban Maria, Zerroug Mohamed Mihoub
The aerial parts of Helichrysum italicum (Roth) G. Don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. The essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). The most oxygenated sesquiterpene compounds were α-Cedrene (13...
July 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28723191/highly-enriched-controllable-continuous-aerosol-sampling-using-inertial-microfluidics-and-its-application-to-real-time-detection-of-airborne-bacteria
#5
Jeongan Choi, Seung Chan Hong, Woojin Kim, Jae Hee Jung
We report a novel microfluidic technique for sampling of aerosols into liquids. The two-phase fluid, sampling air and collecting liquid, forms a stratified flow in the curved microchannel. By passing fluids through the curved region, the particles are transferred from air into the liquid phase by the particle centrifugal and drag forces. This microfluidic-based aerosol-into-liquid sampling system, called the MicroSampler, is driven by particle inertial differences. To evaluate the physical particle collection efficiency of the MicroSampler, we used standard polystyrene-latex (PSL) particles ranging in size from 0...
April 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28721612/interface-effect-of-natural-precipitated-dust-on-the-normal-flora-of-escherichia-coli-and-staphylococcus-epidermidis
#6
Jianjun Deng, Faqin Dong, Qunwei Dai, Tingting Huo, Ji Ma, Xu Zhang, Jie Yang
This study aimed to evaluate the interface effect between five types of natural precipitated dust and two normal floras. Five kinds of natural dust (FC-1(#), FC-2(#), FC-15(#), FC-18(#), and FC-21(#)) were collected, and particle size and chemical components were detected by laser particle size analyzer and X-ray fluorescence (XRF). The elements, bacterial count, glucose (GLU) consumption, pH, and three biochemical indicators were measured after being co-cultured with Escherichia coli and Staphylococcus epidermidis in vitro...
July 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28717325/tetracycline-resistance-phenotypes-and-genotypes-of-coagulase-negative-staphylococcal-isolates-from-bubaline-mastitis-in-egypt
#7
K A Abd El-Razik, A A Arafa, R H Hedia, E S Ibrahim
AIM: This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt. MATERIALS AND METHODS: A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotypic and molecular methods (polymerase chain reaction [PCR]). CNS isolates were tested for tetracycline resistance using routine methods and multiplex PCR targeting tetracycline (tet) resistance genes followed by sequencing of positive PCR products and phylogenetic analysis...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28715352/commensal-bacterial-modulation-of-the-host-immune-response-to-ameliorate-pain-in-a-murine-model-of-chronic-prostatitis
#8
Stephen F Murphy, Anthony J Schaeffer, Joseph D Done, Marsha L Quick, Utkucan Acar, Praveen Thumbikat
The human commensal microflora plays an essential role in modulating the immune response to control homeostasis. Staphylococcus epidermidis, a commensal bacterium most commonly associated with the skin exerts such effects locally, modulating local immune responses during inflammation and preventing superinfection by pathogens such as Staphylococcus aureus. Although the prostate is considered by many to be sterile, multiple investigations have shown that small numbers of gram-positive bacterial species such as S...
August 2017: Pain
https://www.readbyqxmd.com/read/28714327/elevated-levels-of-circulating-cell-free-dna-and-neutrophil-proteins-are-associated-with-neonatal-sepsis-and-necrotizing-enterocolitis-in-immature-mice-pigs-and-infants
#9
Duc Ninh Nguyen, Allan Stensballe, Jacqueline Cy Lai, Pingping Jiang, Anders Brunse, Yanqi Li, Jing Sun, Carina Mallard, Tom Skeath, Nicholas D Embleton, Janet E Berrington, Per T Sangild
Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis and specific diagnostic markers are lacking. Circulating cell-free DNA (cfDNA) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. We explored the relation of circulating neutrophil-associated proteins and cfDNA to LOS and/or NEC. Using a clinically relevant preterm pig model of spontaneous LOS and NEC development, we investigated neutrophil-associated proteins and cfDNA in plasma, together with cytokines in gut tissues...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/28714053/the-influence-of-surface-texture-and-wettability-on-initial-bacterial-adhesion-on-titanium-and-zirconium-oxide-dental-implants
#10
Torsten Wassmann, Stefan Kreis, Michael Behr, Ralf Buergers
BACKGROUND: This study aims to investigate bacterial adhesion on different titanium and ceramic implant surfaces, to correlate these findings with surface roughness and surface hydrophobicity, and to define the predominant factor for bacterial adhesion for each material. METHODS: Zirconia and titanium specimens with different surface textures and wettability (5.0 mm in diameter, 1.0 mm in height) were prepared. Surface roughness was measured by perthometer (R a ) and atomic force microscopy, and hydrophobicity according to contact angles by computerized image analysis...
December 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/28713777/a-novel-chimeric-endolysin-with-antibacterial-activity-against-methicillin-resistant-staphylococcus-aureus
#11
Hamed Haddad Kashani, Hossein Fahimi, Yasaman Dasteh Goli, Rezvan Moniri
Cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) and amidase are known as catalytic domains of the bacteriophage-derived endolysin LysK and were previously reported to show lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). In the current study, the in silico design and analysis of chimeric CHAP-amidase model was applied to enhance the stability and solubility of protein, which was achieved through improving the properties of primary, secondary and tertiary structures. The coding gene sequence of the chimeric CHAP-amidase was synthesized and subcloned into the pET-22(+) expression vector, and the recombinant protein was expressed in E...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28712577/bacterial-adherence-to-graft-tissues-in-static-and-flow-conditions
#12
Tiago Rafael Veloso, Jorien Claes, Soetkin Van Kerckhoven, Bartosz Ditkowski, Luis G Hurtado-Aguilar, Stefan Jockenhoevel, Petra Mela, Ramadan Jashari, Marc Gewillig, Marc F Hoylaerts, Bart Meyns, Ruth Heying
BACKGROUND: Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE...
June 16, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28712270/folliculitis-caused-by-staphylococcus-epidermidis
#13
Stefano Veraldi, Cinzia Desimine, Anna Grancini
No abstract text is available yet for this article.
July 13, 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28712037/introduction-of-sample-tubes-with-sodium-azide-as-a-preservative-for-ethyl-glucuronide-in-urine
#14
Marc Luginbühl, Wolfgang Weinmann, Ali Al-Ahmad
Ethyl glucuronide (EtG) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. However, the instability of EtG in urine against bacterial degradation or the post-collectional synthesis of EtG in contaminated samples may cause false interpretation of EtG results in urine samples. This study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism taking place during growth of bacterial colonies...
July 15, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28711311/bacterial-colonization-of-penile-prosthesis-after-its-withdrawal-due-to-mechanical-failure
#15
B Etcheverry-Giadrosich, J Torremadé-Barreda, L Pujol-Galarza, F Vigués-Julià
INTRODUCTION: Prosthetic surgery to treat erectile dysfunction has a risk of infection of up to 3%, but this risk can increase to 18% when the surgery involves replacement. This increased risk of infection is attributed to the bacterial colonization of the prosthesis during the initial surgery. OBJECTIVE: To analyse the presence of germs in the prosthesis that is withdrawn due to mechanical failure (not infection), as well as the surgical results and its progression...
July 12, 2017: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/28707817/evaluation-of-free-or-anchored-antimicrobial-peptides-as-candidates-for-the-prevention-of-orthopaedic-device-related-infections
#16
Francesca D'Este, Debora Oro, Gerard Boix-Lemonche, Alessandro Tossi, Barbara Skerlavaj
The prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. Alpha-helical antimicrobial peptides (AMPs) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. The aim of this study was to evaluate the potential of five different helical AMPs, the cathelicidins BMAP-27 and BMAP-28, their (1-18) fragments and the rationally designed, artificial P19(9/G7) peptide, for the prevention of orthopaedic implant infections...
July 14, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28707417/defining-the-metal-specificity-of-a-multifunctional-biofilm-adhesion-protein
#17
Catherine T Chaton, Andrew B Herr
The accumulation associated protein (Aap) of Staphylococcus epidermidis mediates intercellular adhesion events necessary for biofilm growth. This process depends upon Zn(2+) -induced self-assembly of G5 domains within the B-repeat region of the protein, forming anti-parallel, intertwined protein 'ropes' between cells. Pleomorphism in the Zn(2+) -coordinating residues was observed in previously solved crystal structures, suggesting that the metal binding site might accommodate other transition metals and thereby support dimerization...
July 13, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28705673/global-acquisition-of-genetic-materials-from-different-bacteria-into-the-staphylococcal-cassette-chromosome-elements-of-a-staphylococcus-epidermidis
#18
Huping Xue, Zhaowei Wu, Dandan Qiao, Chao Tong, Xin Zhao
Staphylococcus epidermidis has been suggested as a main reservoir of methicillin-resistant and virulence genes to facilitate evolution of Staphylococcus aureus as a successful pathogen. However, it remains a mystery where and how S. epidermidis obtains numerous genes to serve as the reservoir. A methicillin-resistant S. epidermidis isolate NW32 from a mastitic milk sample was sequenced and its staphylococcal cassette chromosome (SCC) elements were characterized in this study. The SCC composite island covered 3...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28704823/different-behavior-and-response-of-staphylococcus-epidermidis-and-streptococcus-pneumoniae-to-a-ventriculoperitoneal-shunt-an-in-vitro-study
#19
Vahid Heidari, Zohreh Habibi, Ahmadreza Hojjati Marvasti, Zahra Ebrahim Soltani, Negin Naderian, Parvin Tanzifi, Farideh Nejat
BACKGROUND: Pneumococcal shunt infection is a rare event. There is no consensus on the therapeutic management of this kind of shunt infection according to literature reviews, and it seems to be different from infection with Staphylococcus epidermidis. We studied 2 shunted patients with pneumococcal meningitis, both of whom were treated with only antibiotics. The management of these cases seems to be different from that of shunt catheter infection due to these bacteria. We conducted a laboratory study to show the different behavior of pneumococcus compared to S...
July 14, 2017: Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28704596/silver-nanoparticles-functionalized-with-ampicillin
#20
Agnieszka Rogowska, Katarzyna Rafińska, Paweł Pomastowski, Justyna Walczak, Viorica Railean-Plugaru, Magdalena Buszewska-Forajta, Bogusław Buszewski
The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. To confirm the occurrence of silver nanoparticles functionalization, FTIR, MALDI-TOF MS, and DLS analysis and zeta potential measurements were performed...
July 13, 2017: Electrophoresis
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