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Xylt-II

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https://www.readbyqxmd.com/read/19289103/analysis-of-xylosyltransferase-ii-binding-to-the-anticoagulant-heparin
#1
Javier Carrera Casanova, Michael Ambrosius, Joachim Kuhn, Knut Kleesiek, Christian Götting
The key enzymes in the biosynthetic pathway of glycosaminoglycan production are represented by the human xylosyltransferase I and its isoform II (XylT-I and XylT-II). The glycosaminoglycan heparin interacts with a variety of proteins, thereby regulating their activities, also those of xylosyltransferases. The identification of unknown amino acids responsible for heparin-binding of XylT-II was addressed in this study. Thus, six XylT-II fragments were designed as fusion proteins with MBP and we received soluble and purified MBP/XylT-II from Escherichia coli...
May 22, 2009: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/19100717/first-in-gel-detection-and-purification-of-human-xylosyltransferase-ii
#2
Javier Carrera Casanova, Christina Roch, Joachim Kuhn, Knut Kleesiek, Christian Götting
Human xylosyltransferases I and II (XylT-I, XylT-II) are key enzymes in glycosaminoglycan biosynthesis. Knowledge about the in vivo molecular weight, oligomeric state or turnover number are essential characteristics which have been addressed in this study. XylT-II was purified from Pichia pastoris by fractionated ammonium sulfate precipitation, heparin affinity and ion exchange chromatography. XylT-II was purified over 7000-fold with a final yield of 2.6%. By utilizing mass spectra analysis we can prove its first in-gel detection showing a migration pattern behavior that confirms its in silico molecular weight of 95...
February 6, 2009: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/17003309/the-xylosyltransferase-i-gene-polymorphism-c-343g-t-p-a125s-is-a-risk-factor-for-diabetic-nephropathy-in-type-1-diabetes
#3
Sylvia Schön, Christian Prante, Claudia Bahr, Lise Tarnow, Joachim Kuhn, Knut Kleesiek, Christian Götting
OBJECTIVE: Xylosyltransferase I (XT-I) is the chain-initiating enzyme in the biosynthesis of proteoglycans in basement membranes. It catalyzes the transfer of xylose to selected serine residues in the core protein. The XYLT-II gene codes for a protein highly homologous to XT-I. Proteoglycans are important components of basement membranes and are responsible for their permeability properties. Type 1 diabetic patients have an altered proteoglycan metabolism, which results in microvascular complications...
October 2006: Diabetes Care
https://www.readbyqxmd.com/read/16571645/polymorphisms-in-the-xylosyltransferase-genes-cause-higher-serum-xt-i-activity-in-patients-with-pseudoxanthoma-elasticum-pxe-and-are-involved-in-a-severe-disease-course
#4
S Schön, V Schulz, C Prante, D Hendig, C Szliska, J Kuhn, K Kleesiek, C Götting
BACKGROUND: Pseudoxanthoma elasticum (PXE) is a heritable connective tissue disorder caused by mutations in the ABCC6 gene. Fragmentation of elastic fibres and deposition of proteoglycans result in a highly variable clinical picture. The altered proteoglycan metabolism suggests that enzymes from this pathway function as genetic co-factors in the severity of PXE. Therefore, we propose the XYLT genes encoding xylosyltransferase I (XT-I) as the chain-initiating enzyme in the biosynthesis of proteoglycans and the highly homologous XT-II as potential candidate genes...
September 2006: Journal of Medical Genetics
https://www.readbyqxmd.com/read/16569644/cloning-and-recombinant-expression-of-active-full-length-xylosyltransferase-i-xt-i-and-characterization-of-subcellular-localization-of-xt-i-and-xt-ii
#5
Sylvia Schön, Christian Prante, Claudia Bahr, Joachim Kuhn, Knut Kleesiek, Christian Götting
Xylosyltransferase I (XT-I) catalyzes the transfer of xylose from UDP-xylose to serine residues in proteoglycan core proteins. This is the first and apparently rate-limiting step in the biosynthesis of the tetrasaccharide linkage region in glycosaminoglycan-containing proteoglycans. The XYLT-II gene codes for a highly homologous protein, but its physiological function is not yet known. Here we present for the first time the construction of a vector encoding the full-length GFP-tagged human XT-I and the recombinant expression of the active enzyme in mammalian cells...
May 19, 2006: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/16376579/mutational-and-functional-analyses-of-xylosyltransferases-and-their-implication-in-osteoarthritis
#6
S Schön, G Huep, C Prante, S Müller, R Christ, F-W Hagena, J Kuhn, K Kleesiek, C Götting
OBJECTIVE: The hallmark in osteoarthritis (OA) is the loss of proteoglycans (PGs) in articular cartilage (AC). Xylosyltransferase I (XT-I) catalyzes the transfer of xylose to serine residues in the core protein and initiates the biosynthesis of PGs in AC. The XYLT-II gene encodes a highly homologous protein but its biological function is not yet known. Here we investigate for the first time genetic variations in the XYLT-genes and serum XT-I activities and their implication in OA. METHODS: Denaturing high-performance liquid chromatography (DHPLC) was used for the screening of the XYLT-genes in 49 OA patients...
May 2006: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/16164625/impact-of-polymorphisms-in-the-genes-encoding-xylosyltransferase-i-and-a-homologue-in-type-1-diabetic-patients-with-and-without-nephropathy
#7
COMPARATIVE STUDY
Sylvia Schön, Christian Prante, Sandra Müller, Manuela Schöttler, Lise Tarnow, Joachim Kuhn, Knut Kleesiek, Christian Götting
BACKGROUND: Xylosyltransferase I (XT-I) is the chain-initiating enzyme in the biosynthesis of heparan sulfate proteoglycans (HSPGs). It catalyses the transfer of xylose to specific serine residues in the core protein. The XYLT-II gene codes for a protein highly homologous to the XT-I but its biologic function is not yet known. HSPGs are thought to play an important role in the permeability properties of the glomerular basement membrane (GBM) and thus the xylotransferase genes might be potential candidate genes predisposing to diabetic nephropathy in type 1 diabetic patients...
October 2005: Kidney International
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