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https://www.readbyqxmd.com/read/28214914/the-impact-of-bitter-taste-receptor-genetics-on-culturable-bacteria-in-chronic-rhinosinusitis
#1
D I Rom, J M Christensen, R Alvarado, R Sacks, R J Harvey
BACKGROUND: Extra-oral bitter taste receptors have been associated with innate bacterial defence mechanisms. Genetic variation in T2R38 functionality has been shown to be associated with susceptibility to upper respiratory tract infections and chronic rhinosinusitis (CRS). We sought to independently assess the influence of bitter taste receptor genotype on the presence of culturable bacteria in the sinuses. METHODOLOGY: A cross-sectional analysis of patients with CRS undergoing surgery was performed...
February 19, 2017: Rhinology
https://www.readbyqxmd.com/read/28214695/the-effects-of-atmospheric-pressure-cold-plasma-treatment-on-microbiological-physical-chemical-and-sensory-characteristics-of-vacuum-packaged-beef-loin
#2
A Bauer, Y Ni, S Bauer, P Paulsen, M Modic, J L Walsh, F J M Smulders
Effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (ACP) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. Exposure of non-covered beef samples under high power ACP conditions resulted in increased a*, b*, Chroma and Hue values, but ACP treatment of packaged loins did not impact colour (L*, a*, b*, Chroma, Hue), lipid peroxidation, sarcoplasmic protein denaturation, nitrate/nitrite uptake, or myoglobin isoform distribution...
February 8, 2017: Meat Science
https://www.readbyqxmd.com/read/28214599/hctoll3-was-involved-in-anti-vibrio-defense-in-freshwater-pearl-mussel-hyriopsis-cumingii
#3
Hong-Wei Zhang, Ying Huang, Xin Man, Yue Wang, Kai-Min Hui, Shao-Wu Yin, Xiao-Wen Zhang
Toll-like receptors (TLRs) play an important role in the activation of innate immune response but their functions in bivalves remain largely unknown. In this study, we identified a TLR from the freshwater pearl mussel Hyriopsis cumingii (HcToll3) and investigated its functions in immunity. The full-length cDNA of HcToll3 is 3852 bp and includes an open reading frame (ORF) of 3228 bp that encodes a polypeptide of 1075 amino acids. The predicted HcToll3 protein shares similar structural characteristics with other known Toll family proteins...
February 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28214598/three-stats-are-involved-in-the-regulation-of-the-expression-of-antimicrobial-peptides-in-the-triangle-sail-mussel-hyriopsis-cumingii
#4
Yun-Jia Dai, Kai-Min Hui, Ying-Hao Zhang, Yan Liu, Yu-Qing Wang, Li-Juan Zhao, Li Lin, Lian-Qin Chai, Shun Wei, Jiang-Feng Lan
Janus kinase (Jak) and signal transducers and activators of transcription (STAT) signaling pathway is associated in antiviral and antibacterial immune response. Previous studies primarily investigated the function of STATs in mammals. For most invertebrates, only one STAT was found in each species, such as STAT92E was found in Drosophila melanogaster. The studies, which focus on the functional difference between various STATs in the same species of invertebrate, are limited. In the present study, three STATs (HcSTAT1, HcSTAT2 and HcSTAT3) were identified in triangle shell pearl mussel, Hyriopsis cumingii...
February 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28214466/antimicrobial-activity-of-hl-60-cells-compared-to-primary-blood-derived-neutrophils-against-staphylococcus-aureus
#5
Ragheda Yaseen, Stefanie Blodkamp, Petra Lüthje, Friederike Reuner, Lena Völlger, Hassan Y Naim, Maren von Köckritz-Blickwede
BACKGROUND: The human leukemia cell line HL-60 is considered an alternative cell culture model to study neutrophil differentiation and migration. The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutrophils to investigate the interaction of neutrophils with Staphylococcus aureus. METHODS: For this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen...
February 19, 2017: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/28214190/biofilm-formation-by-staphylococcus-aureus-isolates-from-a-dental-clinic-in-konya-turkey
#6
Emrah Torlak, Emre Korkut, Ali T Uncu, Yağmur Şener
The ability of Staphylococcus aureus to form biofilm is considered to be a major virulence factor influencing its survival and persistence in both the environment and the host. Biofilm formation in S. aureus is most frequently associated with production of polysaccharide intercellular adhesion by ica operon-encoded enzymes. The present work aimed at evaluating the in vitro biofilm production and presence of the icaA and icaD genes in S. aureus isolates from a dental clinic in Konya, Turkey. The surfaces of inanimate objects were sampled over a period of six months...
February 14, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28214187/microbiological-analysis-antimicrobial-activity-and-heavy-metals-content-of-jordanian-ma-in-hot-springs-water
#7
Muhamad Ali K Shakhatreh, Jacob H Jacob, Emad I Hussein, Majed M Masadeh, Safwan M Obeidat, Abdul-Salam F Juhmani, Mutaz A Abd Al-Razaq
Ma'in hot springs are known as sites of balneotherapy. However, little is known about their microbiology and chemistry. In this study, we aim at evaluating the antimicrobial activity of Ma'in hot-springs water (MHSW), studying its microbiology, and determining its physicochemical properties including the heavy metals content. Therefore, water samples were collected from Ma'in hot springs and tested for antimicrobial activity using agar diffusion method. Water was then cultivated on nutrient agar to isolate and identify the dominant bacteria by chemical and molecular methods...
February 14, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28214181/-adult-immunisation-general-points-hot-topics-and-perspectives
#8
P Loubet, O Launay
Vaccination in immunocompetent adult mainly concerns booster vaccination against diphtheria, tetanus, polio and pertussis. Some chronic diseases may also require the achievement of pneumococcal and influenza vaccines. In addition, from the age of 65, annual influenza vaccination as well as one dose of a live attenuated shingles vaccine between 64 and 75 years are recommended. Immunocompromised adults, due to the increased risk of serious infections responsible of significant morbidity and mortality, are particularly concerned by vaccination...
February 14, 2017: La Revue de Médecine Interne
https://www.readbyqxmd.com/read/28213532/susceptibility-and-pk-pd-relationships-of-staphylococcus-aureus-strains-from-ovine-and-caprine-with-clinical-mastitis-against-five-veterinary-fluoroquinolones
#9
J M Serrano-Rodríguez, C Cárceles-García, C M Cárceles-Rodríguez, M L Gabarda, J M Serrano-Caballero, E Fernández-Varón
Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of veterinary fluoroquinolones as enrofloxacin, its metabolite ciprofloxacin, danofloxacin, difloxacin and marbofloxacin against Staphylococcus aureus strains (n=24) isolated from milk of sheep and goats affected by clinical mastitis were evaluated. The authors have used the MIC and MPC, as well as the pharmacokinetic-pharmacodynamic relationships in plasma and milk. MIC values were significantly different between drugs, unlike MPC values...
February 17, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28213334/reactive-oxygen-a-novel-antimicrobial-mechanism-for-targeting-biofilm-associated-infection
#10
REVIEW
Matthew S Dryden, Jonathan Cooke, Rami J Salib, Rebecca E Holding, Timothy Biggs, Ali A Salamat, Raymond N Allan, Rachel S Newby, Fenella Halstead, Beryl Oppenheim, Thomas Hall, Sophie C Cox, Liam M Grover, Zain Al-Hindi, Lilyann Novak-Frazer, Malcolm D Richardson
Reactive oxygen species (ROS) is a novel therapeutic strategy for topical or local application to wounds, mucosa or internal structures where there may be heavy bacterial bioburden with biofilm and chronic inflammation. Bacterial biofilms are a significant problem in clinical settings owing to their increased tolerance towards conventionally prescribed antibiotics and their propensity for selection of further antibacterial resistance. There is therefore a pressing need for the development of alternative therapeutic strategies that can improve antibiotic efficacy towards biofilms...
February 14, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28213234/dna-wrapped-multi-walled-carbon-nanotube-modified-electrochemical-biosensor-for-the-detection-of-escherichia-coli-from-real-samples
#11
Dilsat Ozkan-Ariksoysal, Yasin Ugur Kayran, Fethiye Ferda Yilmaz, Anton Alexandru Ciucu, Iulia Gabriela David, Vasile David, Mine Hosgor-Limoncu, Mehmet Ozsoz
This paper introduces DNA-wrapped multi-walled carbon nanotube (MWCNT)-modified genosensor for the detection of Escherichia coli (E. coli) from polymerase chain reaction (PCR)-amplified real samples while Staphylococcus aureus (S. aureus) was used to investigate the selectivity of the biosensor. The capture probe specifically recognizing E. coli DNA and it was firstly interacted with MWCNTs for wrapping of single-stranded DNA (ssDNA) onto the nanomaterial. DNA-wrapped MWCNTs were then immobilised on the surface of disposable pencil graphite electrode (PGE) for the detection of DNA hybridization...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28212345/efficient-catalytic-oxidation-of-3-arylthio-and-3-cyclohexylthio-lapachone-derivatives-to-new-sulfonyl-derivatives-and-evaluation-of-their-antibacterial-activities
#12
Mariana F do C Cardoso, Ana T P C Gomes, Caroline Dos S Moreira, Mário M Q Simões, Maria G P M S Neves, David R da Rocha, Fernando de C da Silva, Catarina Moreirinha, Adelaide Almeida, Vitor F Ferreira, José A S Cavaleiro
New sulfonyl-lapachones were efficiently obtained through the catalytic oxidation of arylthio- and cyclohexylthio-lapachone derivatives with hydrogen peroxide in the presence of a Mn(III) porphyrin complex. The antibacterial activities of the non-oxidized and oxidized lapachone derivatives against the Gram-negative bacteria Escherichia coli and the Gram-positive bacteria Staphylococcus aureus were evaluated after their incorporation into polyvinylpyrrolidone (PVP) micelles. The obtained results show that the PVP-formulations of the lapachones 4b-g and of the sulfonyl-lapachones 7e and 7g reduced the growth of S...
February 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28212143/staphylococcus-aureus-infections-some-second-thoughts
#13
Willem J B van Wamel
PURPOSE OF REVIEW: Staphylococcus aureus (S. aureus) is well known for its ability to cause life-threatening infections. On the other hand, this bacterium can thrive as a commensal on and in human tissues without causing much problems. How big a threat is S. aureus actually? Furthermore, commensalism is associated with biofilms, where can we find them, and which natural and artificial components activate biofilm formation? RECENT FINDINGS: Recent findings on S. aureus carriage on skin, mucosa, and in wounds indicate the presence of large numbers of S...
February 15, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28212032/chrodrimanins-k-n-and-related-meroterpenoids-from-the-fungus-penicillium-sp-scs-kfd09-isolated-from-a-marine-worm-sipunculus-nudus
#14
Fan-Dong Kong, Qing-Yun Ma, Sheng-Zhuo Huang, Pei Wang, Jun-Feng Wang, Li-Man Zhou, Jing-Zhe Yuan, Hao-Fu Dai, You-Xing Zhao
Six new meroterpenoids, chrodrimanins K-N (1-4), including two uncommon chlorinated ones (1 and 2), and verruculides B2 (5) and B3 (6), as well as seven known ones (7-13), were isolated from the fermentation broth of Penicillium sp. SCS-KFD09 isolated from a marine worm, Sipunculus nudus, from Haikou Bay, China. The structures including the absolute configurations of the new compounds were unambiguously elucidated by spectroscopic data, X-ray diffraction analysis, and ECD spectra analysis along with quantum ECD calculations...
February 17, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28211298/kinneretia-thg-sqi4-mediated-biosynthesis-of-silver-nanoparticles-and-its-antimicrobial-efficacy
#15
Hina Singh, Juan Du, Tae-Hoo Yi
Simple, facile, effective approach for the biosynthesis of silver nanoparticles using Kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. Kinneretia THG-SQI4 has been isolated from soil sample collected from Shangqui, China. The synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (UV-vis), field emission transmission electron microscopy (FE-TEM), energy dispersive X-ray spectroscopy (EDX), elemental mapping, selected area diffraction pattern, and X-ray diffraction (XRD)...
May 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28210463/frequency-of-inducible-clindamycin-resistance-among-gram-positive-cocci-in-a-tertiary-hospital-tehran-iran
#16
Hiva Saffar, Afsaneh Rajabiani, Alireza Abdollahi, Shirin Habibi, Zohreh Baseri
BACKGROUND AND OBJECTIVES: Accurate designation of antimicrobial susceptibility pattern of the infecting microorganisms is an important crucial factor in making appropriate therapeutic decisions. Macrolide, lincosamide and streptogramin B antibiotics are in a family, reserved as an alternative approach in treatment of resistant Gram positive cocci. Amongst them, clindamycin has been considered as the preferred agent due to its excellent pharmacokinetic properties. The inducible resistance to clindamycin in Gram positive staphylococci and streptococci cannot be recognized by routine broth or agar based susceptibility tests and D-zone testing is necessary...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28210352/risk-of-skin-and-soft-tissue-infections-among-children-found-to-be-staphylococcus-aureus-mrsa-usa300-carriers
#17
Lilly Cheng Immergluck, Shabnam Jain, Susan M Ray, Robert Mayberry, Sarah Satola, Trisha Chan Parker, Keming Yuan, Anaam Mohammed, Robert C Jerris
INTRODUCTION: The purpose of this study was to examine community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) carriage and infections and determine risk factors associated specifically with MRSA USA300. METHODS: We conducted a case control study in a pediatric emergency department. Nasal and axillary swabs were collected, and participants were interviewed for risk factors. The primary outcome was the proportion of S. aureus carriers among those presenting with and without a skin and soft tissue infection (SSTI)...
February 2017: Western Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28209540/in-vitro-characterization-of-ph-sensitive-azithromycin-loaded-methoxy-poly-ethylene-glycol-block-poly-aspartic-acid-graft-imidazole-micelles
#18
Fangfang Teng, Peizong Deng, Zhimei Song, Feilong Zhou, Runliang Feng, Na Liu
In order to improve azithromycin's antibacterial activity in acidic medium, monomethoxy poly (ethylene glycol)-block-poly (aspartic acid-graft-imidazole) copolymer was synthesized through allylation, free radical addition, ring-opening polymerization and amidation reactions with methoxy poly (ethylene glycol) as raw material. Drug loading capacity and encapsulation efficiency of azithromycin-loaded micelles prepared via thin film hydration method were 11.58±0.86% and 96.06±1.93%, respectively. The drug-loaded micelles showed pH-dependent property in the respects of particle size, zeta potential at the range of pH 5...
February 9, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28209343/a-clinical-trial-of-intradermal-and-intramuscular-seasonal-influenza-vaccination-in-patients-with-atopic-dermatitis
#19
Donald Y M Leung, Brett Jepson, Lisa A Beck, Jon M Hanifin, Lynda C Schneider, Amy S Paller, Katherine Monti, Gloria David, Jennifer Canniff, Margarita Gomez Lorenzo, Adriana Weinberg
BACKGROUND: Antibody responses to the inactivated seasonal influenza vaccine in patients with atopic dermatitis (AD) have not been carefully characterized. OBJECTIVE: The primary objective of this study was to compare antibody responses to intradermal vaccination in participants with moderate/severe AD with those in nonatopic participants. Secondary objectives were to evaluate the effect of route of administration, Staphylococcus aureus skin colonization, and disease severity on vaccine response...
January 31, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28209139/stroke-caused-by-an-inflammatory-thrombus-a-case-report
#20
Kai Shan, Wei Guo
BACKGROUND: Stroke is the leading cause of mortality and disability worldwide. Several definite risk factors have been identified for stroke, although infectious factors might also contribute to stroke episodes through increased susceptibility or direct induction. CASE PRESENTATION: A 46-year-old Chinese male initially presented with fever, headache, and impaired memory and developed disturbance of consciousness after admission. A clinical diagnosis of Staphylococcus aureus sepsis, massive cerebral infarction and haemorrhagic transformation (left internal carotid arterial system, inflammatory thrombus) were made based on brain radiography, blood culture and postoperative pathological examinations...
February 16, 2017: BMC Neurology
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