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cancer cell migration

W Su, Y F Wang, F Wang, H J Yang, H Y Yang
Objective: To investigate the effect of long non-coding RNA highly upregulated in liver cancer (lncRNA HULC) on the biological behavior of oral squamous cell carcinoma (OSCC) cell lines. Methods: A total of thirty patients with oral squamous cell carcinoma at Peking University Shenzhen Hospital from March 2017 to March 2018 were enrolled in this study. OSCC and adjacent tissues were extracted during tumor extensive resection. Quantitative real-time PCR (qPCR) was used to detect the expression levels of lncRNA HULC in OSCC and paracancerous tissues and OSCC cell lines...
November 9, 2018: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Alexandre Berquand, Marie Meunier, Jessica Thevenard Devy, Corinne Ivaldi, Océane Campion, Stéphane Dedieu, Michael Molinari, Jérôme Devy
To get more insight into the role of LRP-1 in the mechanism of tumor progression in triple negative breast cancer. Atomic force microscopy, videomicroscopy, confocal microscopy and Rho-GTPAse activity assay were used on MDA-MB-231 and LRP-1-silenced cells. Silencing of LRP-1 in MDA-MB-231 cells was shown to led to a dramatic increase in the Young's modulus in parallel to a spectacular drop in membrane extension dynamics as well as a decrease in the cells migration abilities on both collagen I and fibronectin substrates...
November 9, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
Jian Fang, Han Hong, Xiaofeng Xue, Xinguo Zhu, Linhua Jiang, Mingde Qin, Hansi Liang, Ling Gao
In the present study, two circular RNA (circRNA) expression profiles in paired gastric cancer (GC) tissues from the GEO database were examined. We identified a novel circRNA, has_circ_0001461, which we termed circFAT1(e2). We verified that circFAT1(e2) was significantly downregulated in GC tissues and cell lines and was correlated with overall survival of GC patients. Fluorescence in situ hybridization (FISH) analysis showed that circFAT1(e2) was distributed in the cytoplasm of GC cells, as well as in the nucleus...
November 9, 2018: Cancer Letters
Florian Kleinegger, Eva Hofer, Christina Wodlej, Nicole Golob-Schwarzl, Anna Maria Birkl-Toeglhofer, Alexander Stallinger, Johannes Petzold, Anna Orlova, Stefanie Krassnig, Robert Reihs, Tobias Niedrist, Harald Mangge, Young Nyun Park, Michael Thalhammer, Ariane Aigelsreiter, Sigurd Lax, Christoph Garbers, Peter Fickert, Stefan Rose-John, Richard Moriggl, Beate Rinner, Johannes Haybaeck
Biliary tract cancer (BTC) represents a malignant tumor of the biliary tract including cholangiocarcinoma (CCA) and the carcinoma of the gallbladder (GBC) with a 5-year survival rate between 5 and 18% due to late diagnosis and rapid disease progression. Chronic inflammation is one of the main risk factors for CCA and GBC in particular. IL-6, as a mediator of inflammation, can act through a membrane-bound receptor alpha-chain (mIL-6R, "IL-6 classic signaling") or via soluble forms (sIL-6R, "IL-6 trans-signaling")...
November 9, 2018: Biochimica et biophysica acta. Molecular basis of disease
Nicole Pinto, Stephenie D Prokopec, Frederick Vizeacoumar, Karlee Searle, Matthew Lowerison, Kara M Ruicci, John Yoo, Kevin Fung, Danielle MacNeil, Jim C Lacefield, Hon S Leong, Joe S Mymryk, John W Barrett, Alessandro Datti, Paul C Boutros, Anthony C Nichols
Anaplastic thyroid cancer (ATC) is a rare and lethal human malignancy with no known effective therapies in the majority of cases. Despite the use of conventional treatments such as chemotherapy, radiation and surgical resection, this disease remains almost universally fatal. In the present study, we identified the JAK2 inhibitor Lestaurtinib as a potent compound when testing against 13 ATC cell lines. Lestaurtinib demonstrated a potent antiproliferative effect in vitro at nanomolar concentrations. Furthermore, Lestaurtinib impeded cell migration and the ability to form colonies from single cells using scratch-wound and colony formation assays, respectively...
2018: PloS One
Huiying Li, Huaigu Yang, Peng Li, Yizhen Wang, Guoxin Huang, Lei Xing, Jiaqi Wang, Nan Zheng
To investigate the effect of heat treatment on the anti-tumor activity of lactoferrin in colon cancer cells and colon tumors, the HT-29 (human intestinal epithelial tumor cell) cell line was exposed to lactoferrin and various heat treatments. The impacts on cell proliferation, invasion as well as migration was observed in vitro, and nude mice bearing HT29 tumors were administered lactoferrin and underwent various heat treatments in vivo. In the HT29 cell proliferation test using transwell and scratch analyses, lactoferrin (20 mg/mL) without or with heat treatment (50℃ and 70℃) significantly inhibited cell proliferation, migration and invasion (compared with the control, p<0...
November 12, 2018: Journal of Agricultural and Food Chemistry
Tanja Eisemann, Barbara Costa, Patrick N Harter, Wolfgang Wick, Michel Mittelbronn, Peter Angel, Heike Peterziel
Background: Treatment options of glioblastoma, the most aggressive primary brain tumor with frequent relapses and high mortality, are still very limited urgently calling for novel therapeutic targets. Expression of the glycoprotein podoplanin correlates with poor prognosis in various cancer entities including glioblastoma. Furthermore, podoplanin has been associated with tumor cell migration and proliferation in vitro; however, experimental data on its function in gliomagenesis in vivo is still missing...
November 10, 2018: Neuro-oncology
Jian Zhou, Shutao Zheng, Tao Liu, Qing Liu, Yumei Chen, Rong Ma, Doudou Tan, Xiaomei Lu
Although M2 tumour-associated macrophages (M2 TAMs) have been shown to be associated with the progression and metastasis of breast cancer, their role in oesophageal squamous cell carcinoma (ESCC) remains less well understood. Therefore, to understand the clinicopathological significance of infiltrated M2 TAMs in ESCC, statistical analysis was performed after immunohistochemical evaluation of CD163 expression, a well-accepted surface marker of M2 TAMs in ESCC. To gain insight into the effect of M2 TAMs, ESCC cell lines Eca109 and KYSE150 cells were co-cultured with M2 TAMs artificially induced from THP-1 cells...
November 12, 2018: Histology and Histopathology
S M Anisul Islam, Rekha Patel, Raja Reddy Bommareddy, Khandker Mohammad Khalid, Mildred Acevedo-Duncan
Colorectal cancer (CRC) is the third most common cancer in both men and women in the United States. The exact mechanism of CRC cells metastasis is poorly understood, therefore, identification of novel, targetable markers of the disease progression is necessary. Actin polymerization is thought to be an initial step in the cancer cell motility cycle which drives the formation of cell protrusions that define the direction of migration and initiates cell crawling. Cofilin is a significant actin-regulating molecule that can regulate the migration of cancer cells by the formation of lamellipodia and filopodia, however, little is known about the upstream regulation of cofilin...
November 10, 2018: Cell Adhesion & Migration
Chunyan Fang, Jian Zhang, Hanlin Yang, Lili Peng, Kun Wang, Yanjie Wang, Xin Zhao, Huijie Liu, Chunhui Dou, Lihong Shi, Chunling Zhao, Shujuan Liang, Daqi Li, Xuejian Wang
Overexpression of leucine aminopeptidase 3 (LAP3) is involved in proliferation, migration, and invasion of several tumor cells and plays a crucial role in tumor metastasis. However, the related mechanism remains unknown. In this study, we used MDA-MB-231 and MCF7 breast cancer cell lines to explore the role of LAP3 in the regulation of cancer cell migration and invasion by employing the natural LAP3 inhibitor bestatin and a lentivirus vector that overexpresses or knocks down LAP3. Bestatin inhibited tumor cell migration and invasion in a dose-dependent manner...
November 11, 2018: Journal of Cellular Biochemistry
Xiaoguang Zhou, Peng Zhang, Hu Han, Hongen Lei, Xiaodong Zhang
OBJECTIVES: Bladder cancer (BCa) is the most common malignant tumor in the urinary system. Growing evidence suggests that as a tumor suppressor gene, hypermethylated in cancer 1 (HIC1) is correlated with various malignancies in the modulation of tumor progression. This study aims to investigate the effect of HIC1 on regulating the proliferation, migration, and invasion of BCa. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot (WB) were used to evaluate the expression of HIC1 messenger RNA and protein in human BCa tissues and cells...
November 11, 2018: Journal of Cellular Biochemistry
Dong-Hui Xu, Guo-Nan Chi, Cong-Hai Zhao, Dong-Yuan Li
Glioma is a common primary brain tumor with high mortality rate and poor prognosis. Long noncoding RNA maternally expressed gene 3 (MEG3) is a tumor suppressor in diverse cancer types. However, the role of MEG3 in glioma remains unclear. We aimed to explore the effects of MEG3 on U251 cells as well as the underlying mechanisms. U251 cells were stably transfected with different recombined plasmids to overexpress or silence MEG3. Effects of aberrantly expressed MEG3 on cell viability, migration, apoptosis, expressions of apoptosis-associated and autophagy-associated proteins, and phosphorylated levels of key kinases in the PI3K/AKT/mTOR pathway were all evaluated...
November 11, 2018: Journal of Cellular Biochemistry
Yu-Heng Vivian Ma, Liangcheng Xu, Xueting Mei, Kevin Middleton, Lidan You
Bone metastases occur in 65% to 75% of patients with advanced breast cancer and significantly worsen their survival and quality of life. We previously showed that conditioned medium (CM) from osteocytes stimulated with oscillatory fluid flow, mimicking bone mechanical loading during routine physical activities, reduced the transendothelial migration of breast cancer cells. Endothelial cells are situated at an ideal location to mediate signals between osteocytes in the bone matrix and metastasizing cancer cells in the blood vessels...
November 11, 2018: Journal of Cellular Biochemistry
Xiaorui Chen, Chang Li, Rongjie Cheng, Zhuo Gao, Xiangbo Meng, Chen Sun, Chenxi Liang, Lianjun Ma, Yanqing Liu
Gastric cancer (GC) is a worldwide health problem. Uncovering the underlining molecular mechanisms of GC is of vital significance. Here, we identified a novel oncogene WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) in GC. WWP1 could promote GC cell proliferation and migration in vitro and expedite GC growth in vivo. We also found out two microRNAs (miRNAs): miR-129-5p and -3p could both target WWP1. Interestingly, miR-129-5p bound to the CDS region of WWP1 mRNA. The miR-129 pairs (miR-129-5p and -3p) play pivotal roles in GC to suppress its proliferation and migration in vitro and slow down GC growth in vivo by repressing WWP1...
November 11, 2018: Journal of Cellular Biochemistry
Jinbo Zhao, Dehua Zhu, Xiupeng Zhang, Yong Zhang, Jianping Zhou, Ming Dong
BACKGROUND: The roles of TMEM206, a new transmembrane protein, in cancer, including colorectal cancer (CRC), are unknown. Related family members, including TMEM16A, TMEM132A, and TMEM176B, have been shown to be involved in various biological behaviors. In addition, TMEM88 has been reported to promote non-small-cell lung cancer. In this study, we examined the roles of TMEM206 in CRC. METHOD: Real-time reverse transcription polymerase chain reaction was used to measure TMEM206 messenger RNA (mRNA) levels in clinical specimens and transfected cell lines...
November 11, 2018: Journal of Cellular Physiology
Yan Zhang, Hongying Xu, Junhao Mu, Shuliang Guo, Lin Ye, Dairong Li, Weiyan Peng, Xiaoqian He, Tingxiu Xiang
Lung cancer is the most frequently diagnosed cancer worldwide. Epigenetic regulation contributes to lung cancer pathogenesis. The ADAMTS18 tumor suppressor gene is inactivated in some cancers, but its involvement in lung cancer has not been shown. Immunohistochemistry, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and methylation-specific PCR were used to assay ADAMTS18 expression and promoter methylation in lung tumor tissues and adjacent tissues. Cell viability, transwell, and wound-healing assays, as well as flow cytometry were used to characterize the biological activity of ADAMTS18...
November 11, 2018: Journal of Cellular Physiology
Levent Elmas, Mücahit Secme, Ramazan Mammadov, Umut Fahrioglu, Yavuz Dodurga
Coriander (Coriandrum sativum L.) is such an herb from the Apiaceae family, used both for its medicinal and nutritional properties for many centuries. In this study, the effects of C. sativum extract on gene expression, viability, colony formation, migration, and invasion of PC-3 and LNCaP prostate cancer cell lines have been investigated. The half maximal inhibitory concentration (IC50 ) dose in PC-3 and LNCaP cells was detected to be 2 and 5 mg/mL at the 24th hour, respectively. C. sativum extracts have been observed to cause a significant decrease in the expression of Akt and Bcl-2 in the PC-3 cells and just Akt in LNCaP cells while increasing in the expression of p53, caspase-9, caspase-10, PTEN, DR5, TRADD, PUMA, and NOXA...
November 11, 2018: Journal of Cellular Biochemistry
Xufeng Tian, Lin Zhang, Yan Jiao, Junsheng Chen, Ying Shan, Wenfeng Yang
Circular RNA (circRNA) is a key regulator in the development and progression of human cancers. Previous studies confirmed circRNA-0008717 (circABCB10) as an oncogene in osteosarcoma, but the regulatory effect of circABCB10 in nonsmall cell lung cancer (NSCLC) is still unclear. In the current study, we examined the expression of circABCB10 in different NSCLC cell lines. Bioinformatics analysis, Cell Counting Kit-8 assays, Transwell migration, fluorescein reporting experiments, and xenografts in mice were used to detect the effect of circABCB10 on NSCLC cell proliferation and migration in vitro and tumor growth in vivo...
November 11, 2018: Journal of Cellular Biochemistry
Sumeng Gao, Min Chen, Wei Wei, Xiaoqi Zhang, Mingming Zhang, Yuling Yao, Ying Lv, Tingsheng Ling, Lei Wang, Xiaoping Zou
Cancer cells consume large amounts of glucose to produce lactate, even in the presence of ample oxygen. This phenomenon is called the Warburg effect. c-Myc is an important member of the Myc gene family and is involved in the development of various tumors. It plays an important role in the regulation of tumor energy metabolism, which can regulate glycolysis to promote the Warburg effect in a tumor. Our study aimed to improve the malignant biological behavior by controlling the energy metabolism of gastric cancer through the mTOR/PKM2 and signal transduction and activator 3 (STAT3)/c-Myc signaling pathways through a series of in vitro experiments...
November 11, 2018: Journal of Cellular Biochemistry
Bin Wang, Xiaoting Wang, Diyu Hou, Qian Huang, Weiwu Zhan, Canwei Chen, Jingru Liu, Ruolan You, Jieqiong Xie, Ping Chen, Huifang Huang
Acute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell-derived exosomes can induce angiogenesis, but their role in vascular remodeling during AML is unclear. Here, we found that exosomes secreted by AML cells promoted proliferation and migration and tube-forming activity of human umbilical vein endothelial cells (HUVECs), whereas HUVECs conferred chemoresistance to AML cells...
November 11, 2018: Journal of Cellular Physiology
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