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traction force microscopy

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https://www.readbyqxmd.com/read/28074837/two-layer-elastographic-3-d-traction-force-microscopy
#1
Begoña Álvarez-González, Shun Zhang, Manuel Gómez-González, Ruedi Meili, Richard A Firtel, Juan C Lasheras, Juan C Del Álamo
Cellular traction force microscopy (TFM) requires knowledge of the mechanical properties of the substratum where the cells adhere to calculate cell-generated forces from measurements of substratum deformation. Polymer-based hydrogels are broadly used for TFM due to their linearly elastic behavior in the range of measured deformations. However, the calculated stresses, particularly their spatial patterns, can be highly sensitive to the substratum's Poisson's ratio. We present two-layer elastographic TFM (2LETFM), a method that allows for simultaneously measuring the Poisson's ratio of the substratum while also determining the cell-generated forces...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28024085/modeling-the-functional-role-of-valve-interstitial-cell-stress-fibers
#2
Yusuke Sakamoto, Rachel M Buchanan, Johannah S Adams, Farshid Guilak, Michael S Sacks
The function of the heart valve interstitial cells (VICs) are intimately connected to heart valve tissue remodeling and repair, as well as initiation of pathological processes. Given the complex three-dimensional VIC structure and associated biomechanical behaviors, we developed a VIC computational continuum mechanics model to determine changes in stress fibers contraction and strain rate sensitivity. Novel experimental data was acquired including stress fiber orientation, expression levels of F-actin and α-SMA, as well as microindentation studies...
December 26, 2016: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28008471/microfabricated-tissues-for-investigating-traction-forces-involved-in-cell-migration-and-tissue-morphogenesis
#3
REVIEW
Bryan A Nerger, Michael J Siedlik, Celeste M Nelson
Cell-generated forces drive an array of biological processes ranging from wound healing to tumor metastasis. Whereas experimental techniques such as traction force microscopy are capable of quantifying traction forces in multidimensional systems, the physical mechanisms by which these forces induce changes in tissue form remain to be elucidated. Understanding these mechanisms will ultimately require techniques that are capable of quantifying traction forces with high precision and accuracy in vivo or in systems that recapitulate in vivo conditions, such as microfabricated tissues and engineered substrata...
December 22, 2016: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28002746/frustrated-phagocytic-spreading-of-j774a-1-macrophages-ends-in-myosin-ii-dependent-contraction
#4
Daniel T Kovari, Wenbin Wei, Patrick Chang, Jan-Simon Toro, Ruth Fogg Beach, Dwight Chambers, Karen Porter, Doyeon Koo, Jennifer E Curtis
Conventional studies of dynamic phagocytic behavior have been limited in terms of spatial and temporal resolution due to the inherent three-dimensionality and small features of phagocytosis. To overcome these issues, we use a series of frustrated phagocytosis assays to quantitatively characterize phagocytic spreading dynamics. Our investigation reveals that frustrated phagocytic spreading occurs in phases and is punctuated by a distinct period of contraction. The spreading duration and peak contact areas are independent of the surface opsonin density, although the opsonin density does affect the likelihood that a cell will spread...
December 20, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27994457/nanomechanical-measurement-of-adhesion-and-migration-of-leukemia-cells-with-phorbol-12-myristate-13-acetate-treatment
#5
Zhuo Long Zhou, Jing Ma, Ming-Hui Tong, Barbara Pui Chan, Alice Sze Tsai Wong, Alfonso Hing Wan Ngan
The adhesion and traction behavior of leukemia cells in their microenvironment is directly linked to their migration, which is a prime issue affecting the release of cancer cells from the bone marrow and hence metastasis. In assessing the effectiveness of phorbol 12-myristate 13-acetate (PMA) treatment, the conventional batch-cell transwell-migration assay may not indicate the intrinsic effect of the treatment on migration, since the treatment may also affect other cellular behavior, such as proliferation or death...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27990827/coordination-between-intra-and-extracellular-forces-regulates-focal-adhesion-dynamics
#6
Bibhu Ranjan Sarangi, Mukund Gupta, Bryant L Doss, Nicolas Tissot, France Lam, René-Marc Mège, Nicolas Borghi, Benoît Ladoux
Focal adhesions (FAs) are important mediators of cell-substrate interactions. One of their key functions is the transmission of forces between the intracellular acto-myosin network and the substrate. However, the relationships between cell traction forces, FA architecture, and molecular forces within FAs are poorly understood. Here, by combining Förster resonance energy transfer (FRET)-based molecular force biosensors with micropillar-based traction force sensors and high-resolution fluorescence microscopy, we simultaneously map molecular tension across vinculin, a key protein in FAs, and traction forces at FAs...
December 23, 2016: Nano Letters
https://www.readbyqxmd.com/read/27990663/%C3%AE-v%C3%AE-3-integrins-negatively-regulate-cellular-forces-by-phosphorylation-of-its-distal-npxy-site
#7
Rachel Milloud, Olivier Destaing, Richard de Mets, Ingrid Bourrin-Reynard, Christiane Oddou, Antoine Delon, Irène Wang, Corinne Albigès-Rizo, Martial Balland
BACKGROUND INFORMATION: Integrins are key receptors that allow cells to sense and respond to their mechanical environment. Although they bind the same ligand, β1 and β3 integrins have distinct and cooperative roles in mechanotransduction. RESULTS: Using traction force microscopy on unconstrained cells, we show that deleting β3 causes traction forces to increase, whereas the deletion of β1 integrin results in a strong decrease of contractile forces. Consistently, loss of β3 integrin also induces an increase in β1 integrin activation...
November 7, 2016: Biology of the Cell
https://www.readbyqxmd.com/read/27892536/investigation-of-cell-substrate-adhesion-properties-of-living-chondrocyte-by-measuring-adhesive-shear-force-and-detachment-using-afm-and-inverse-fea
#8
Trung Dung Nguyen, YuanTong Gu
It is well-known that cell adhesion is important in many biological processes such as cell migration and proliferation. A better understanding of the cell adhesion process will shed insight into these cellular biological responses as well as cell adhesion-related diseases treatment. However, there is little research which has attempted to investigate the process of cell adhesion and its mechanism. Thus, this paper aims to study the time-dependent adhesion properties of single living chondrocytes using an advanced coupled experimental-numerical approach...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27768890/rickettsia-sca4-reduces-vinculin-mediated-intercellular-tension-to-promote-spread
#9
Rebecca L Lamason, Effie Bastounis, Natasha M Kafai, Ricardo Serrano, Juan C Del Álamo, Julie A Theriot, Matthew D Welch
Spotted fever group (SFG) rickettsiae are human pathogens that infect cells in the vasculature. They disseminate through host tissues by a process of cell-to-cell spread that involves protrusion formation, engulfment, and vacuolar escape. Other bacterial pathogens rely on actin-based motility to provide a physical force for spread. Here, we show that SFG species Rickettsia parkeri typically lack actin tails during spread and instead manipulate host intercellular tension and mechanotransduction to promote spread...
October 20, 2016: Cell
https://www.readbyqxmd.com/read/27733037/deletion-of-calponin-2-in-mouse-fibroblasts-increases-myosin-ii-dependent-cell-traction-force
#10
M Moazzem Hossain, Guangyi Zhao, Moon-Sook Woo, James H-C Wang, Jian-Ping Jin
Cell traction force (CTF) plays a critical role in controlling cell shape, enabling cell motility, and maintaining cellular homeostasis in many biological processes such as angiogenesis, development, wound healing, and cancer metastasis. Calponin is an actin filament-associated cytoskeletal protein in smooth muscles and multiple types of non-muscle cells. An established biochemical function of calponin is the inhibition of myosin ATPase in smooth muscle cells. Vertebrates have three calponin isoforms. Among them, calponin 2 is expressed in epithelial cells, endothelial cells, macrophages, myoblasts and fibroblasts, and plays a role in regulating cytoskeleton activities such as cell adhesion, migration and cytokinesis...
October 13, 2016: Biochemistry
https://www.readbyqxmd.com/read/27709128/fast-and-robust-fabrication-of-reusable-molds-for-hydrogel-micro-patterning
#11
Richard De Mets, Katharina Hennig, Lionel Bureau, Martial Balland
Mechanical interactions between cells and their microenvironment are crucial for fundamental biological processes ranging from migration to differentiation. This has led, over the last decades, to the development of new ways to culture cells. Living cells are now grown not only on glass coverslips, where they completely lose the mechanical and geometrical constraints coming from their microenvironment, but also on soft patterned substrates that mimic the rigidity and spatial information of their in vivo niches...
October 18, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27708765/prediction-of-traction-forces-of-motile-cells
#12
Clément Roux, Alain Duperray, Valérie M Laurent, Richard Michel, Valentina Peschetola, Claude Verdier, Jocelyn Étienne
When crawling on a flat substrate, living cells exert forces on it via adhesive contacts, enabling them to build up tension within their cytoskeleton and to change shape. The measurement of these forces has been made possible by traction force microscopy (TFM), a technique which has allowed us to obtain time-resolved traction force maps during cell migration. This cell 'footprint' is, however, not sufficient to understand the details of the mechanics of migration, that is how cytoskeletal elements (respectively, adhesion complexes) are put under tension and reinforce or deform (respectively, mature and/or unbind) as a result...
October 6, 2016: Interface Focus
https://www.readbyqxmd.com/read/27681958/confocal-reference-free-traction-force-microscopy
#13
Martin Bergert, Tobias Lendenmann, Manuel Zündel, Alexander E Ehret, Daniele Panozzo, Patrizia Richner, David K Kim, Stephan J P Kress, David J Norris, Olga Sorkine-Hornung, Edoardo Mazza, Dimos Poulikakos, Aldo Ferrari
The mechanical wiring between cells and their surroundings is fundamental to the regulation of complex biological processes during tissue development, repair or pathology. Traction force microscopy (TFM) enables determination of the actuating forces. Despite progress, important limitations with intrusion effects in low resolution 2D pillar-based methods or disruptive intermediate steps of cell removal and substrate relaxation in high-resolution continuum TFM methods need to be overcome. Here we introduce a novel method allowing a one-shot (live) acquisition of continuous in- and out-of-plane traction fields with high sensitivity...
September 29, 2016: Nature Communications
https://www.readbyqxmd.com/read/27531518/cellular-adhesome-screen-identifies-critical-modulators-of-focal-adhesion-dynamics-cellular-traction-forces-and-cell-migration-behaviour
#14
Michiel Fokkelman, Hayri E Balcıoğlu, Janna E Klip, Kuan Yan, Fons J Verbeek, Erik H J Danen, Bob van de Water
Cancer cells migrate from the primary tumour into surrounding tissue in order to form metastasis. Cell migration is a highly complex process, which requires continuous remodelling and re-organization of the cytoskeleton and cell-matrix adhesions. Here, we aimed to identify genes controlling aspects of tumour cell migration, including the dynamic organization of cell-matrix adhesions and cellular traction forces. In a siRNA screen targeting most cell adhesion-related genes we identified 200+ genes that regulate size and/or dynamics of cell-matrix adhesions in MCF7 breast cancer cells...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27500521/engineering-three-dimensional-epithelial-tissues-embedded-within-extracellular-matrix
#15
Alexandra S Piotrowski-Daspit, Celeste M Nelson
The architecture of branched organs such as the lungs, kidneys, and mammary glands arises through the developmental process of branching morphogenesis, which is regulated by a variety of soluble and physical signals in the microenvironment. Described here is a method created to study the process of branching morphogenesis by forming engineered three-dimensional (3D) epithelial tissues of defined shape and size that are completely embedded within an extracellular matrix (ECM). This method enables the formation of arrays of identical tissues and enables the control of a variety of environmental factors, including tissue geometry, spacing, and ECM composition...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27498166/traction-force-dynamics-predict-gap-formation-in-activated-endothelium
#16
Erik T Valent, Geerten P van Nieuw Amerongen, Victor W M van Hinsbergh, Peter L Hordijk
In many pathological conditions the endothelium becomes activated and dysfunctional, resulting in hyperpermeability and plasma leakage. No specific therapies are available yet to control endothelial barrier function, which is regulated by inter-endothelial junctions and the generation of acto-myosin-based contractile forces in the context of cell-cell and cell-matrix interactions. However, the spatiotemporal distribution and stimulus-induced reorganization of these integral forces remain largely unknown. Traction force microscopy of human endothelial monolayers was used to visualize contractile forces in resting cells and during thrombin-induced hyperpermeability...
September 10, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27353427/the-actin-crosslinking-protein-palladin-modulates-force-generation-and-mechanosensitivity-of-tumor-associated-fibroblasts
#17
Mikheil Azatov, Silvia M Goicoechea, Carol A Otey, Arpita Upadhyaya
Cells organize actin filaments into higher-order structures by regulating the composition, distribution and concentration of actin crosslinkers. Palladin is an actin crosslinker found in the lamellar actin network and stress fibers, which are critical for mechanosensing of the environment. Palladin also serves as a molecular scaffold for α-actinin, another key actin crosslinker. By virtue of its close interactions with actomyosin structures in the cell, palladin may play an important role in cell mechanics...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27304456/three-dimensional-reflectance-traction-microscopy
#18
Jihan Kim, Christopher A R Jones, Nicholas Scott Groves, Bo Sun
Cells in three-dimensional (3D) environments exhibit very different biochemical and biophysical phenotypes compared to the behavior of cells in two-dimensional (2D) environments. As an important biomechanical measurement, 2D traction force microscopy can not be directly extended into 3D cases. In order to quantitatively characterize the contraction field, we have developed 3D reflectance traction microscopy which combines confocal reflection imaging and partial volume correlation postprocessing. We have measured the deformation field of collagen gel under controlled mechanical stress...
2016: PloS One
https://www.readbyqxmd.com/read/27235994/substrate-stiffness-and-matrix-composition-coordinately-control-the-differentiation-of-liver-progenitor-cells
#19
Andreas P Kourouklis, Kerim B Kaylan, Gregory H Underhill
Recent approaches have utilized microfabricated platforms to examine combinations of microenvironmental signals that regulate stem and progenitor cell differentiation. However, the majority of these efforts have focused on the biochemical properties of extracellular matrix (ECM) or soluble factors without simultaneously exploring the biomechanical effects of cell-substrate interactions. To address this need, we combined a high-throughput approach for the analysis of combinatorial ECM cues with substrates of modular stiffness and traction force microscopy...
August 2016: Biomaterials
https://www.readbyqxmd.com/read/27232264/non-muscle-myosin-iia-is-critical-for-podocyte-f-actin-organization-contractility-and-attenuation-of-cell-motility
#20
Philip A Bondzie, Hui A Chen, Mei Zhen Cao, Julie A Tomolonis, Fangfang He, Martin R Pollak, Joel M Henderson
Several glomerular pathologies resulting from podocyte injury are linked to genetic variation involving the MYH9 gene, which encodes the heavy chain of non-muscle myosin-IIA (NM-IIA). However, the functional role of NM-IIA has not been studied extensively in podocytes. We hypothesized that NM-IIA is critical for maintenance of podocyte structure and mechanical function. To test this hypothesis, we studied murine podocytes in vitro subjected to blebbistatin inhibition of NM-II activity, or RNA interference-mediated, isoform-specific ablation of Myh9 gene and protein (NM-IIA) or its paralog Myh10 gene and protein (NM-IIB)...
August 2016: Cytoskeleton
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