keyword
MENU ▼
Read by QxMD icon Read
search

traction force microscopy

keyword
https://www.readbyqxmd.com/read/29242157/determination-of-green-s-function-for-three-dimensional-traction-force-reconstruction-based-on-geometry-and-boundary-conditions-of-cell-culture-matrices
#1
Y Du, S C B Herath, Q G Wang, H Asada, P C Y Chen
Cell migration plays a particular important role in the initiation and progression of many physical processes and pathological conditions such as tumor invasion and metastasis. Three-dimensional traction force microscopy (TFM) of high resolution and high accuracy is being developed in an effort to unveil the underlying mechanical process of cell migration in a vivo-like environment. Linear elasticity-based TFM (LETM) as a mainstream approach relies on the Green's function (that relates traction forces to matrix deformation), of which the inherent boundary conditions and geometry of the matrix could remarkably affect the result as suggested by previous 2D studies...
December 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29229906/direction-of-actin-flow-dictates-integrin-lfa-1-orientation-during-leukocyte-migration
#2
Pontus Nordenfelt, Travis I Moore, Shalin B Mehta, Joseph Mathew Kalappurakkal, Vinay Swaminathan, Nobuyasu Koga, Talley J Lambert, David Baker, Jennifer C Waters, Rudolf Oldenbourg, Tomomi Tani, Satyajit Mayor, Clare M Waterman, Timothy A Springer
Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts a specific orientation dictated by the direction of actin flow on the surface of migrating cells. We insert GFP into the rigid, ligand-binding head of the integrin, model with Rosetta the orientation of GFP and its transition dipole relative to the integrin head, and measure orientation with fluorescence polarization microscopy...
December 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/29199833/single-cell-optical-distortion-correction-and-label-free-3d-cell-shape-reconstruction-on-lattices-of-nanostructures
#3
Jürgen Stephan, Felix Keber, Valentin Stierle, Joachim O Rädler, Philipp Paulitschke
Imaging techniques can be compromised by aberrations. Especially when imaging through biological specimens, sample-induced distortions can limit localization accuracy. In particular, this phenomenon affects localization microscopy, traction force measurements, and single-particle tracking, which offer high-resolution insights into biological tissue. Here we present a method for quantifying and correcting the optical distortions induced by single, adherent, living cells. The technique uses periodically patterned gold nanostructures as a reference framework to quantify optically induced displacements with micrometer-scale sampling density and an accuracy of a few nanometers...
December 4, 2017: Nano Letters
https://www.readbyqxmd.com/read/29160067/molecular-tension-probes-for-imaging-forces-at-the-cell-surface
#4
Yang Liu, Kornelia Galior, Victor Pui-Yan Ma, Khalid Salaita
Mechanical forces are essential for a variety of biological processes ranging from transcription and translation to cell adhesion, migration, and differentiation. Through the activation of mechanosensitive signaling pathways, cells sense and respond to physical stimuli from the surrounding environment, a process widely known as mechanotransduction. At the cell membrane, many signaling receptors, such as integrins, cadherins and T- or B-cell receptors, bind to their ligands on the surface of adjacent cells or the extracellular matrix (ECM) to mediate mechanotransduction...
November 21, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29150767/cell-migration-in-1d-and-2d-nanofiber-microenvironments
#5
Horacio M Estabridis, Aniket Jana, Amrinder Nain, David J Odde
Understanding how cells migrate in fibrous environments is important in wound healing, immune function, and cancer progression. A key question is how fiber orientation and network geometry influence cell movement. Here we describe a quantitative, modeling-based approach toward identifying the mechanisms by which cells migrate in fibrous geometries having well controlled orientation. Specifically, U251 glioblastoma cells were seeded onto non-electrospinning Spinneret based tunable engineering parameters fiber substrates that consist of networks of suspended 400 nm diameter nanofibers...
November 17, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29103075/measurement-of-cell-traction-force-with-a-thin-film-pdms-cantilever
#6
Merrel T Holley, Ashkan YekrangSafakar, Mazvita Maziveyi, Suresh K Alahari, Kidong Park
Adherent cells produce cellular traction force (CTF) on a substrate to maintain their physical morphologies, sense external environment, and perform essential cellular functions. Precise characterization of the CTF can expand our knowledge of various cellular processes as well as lead to the development of novel mechanical biomarkers. However, current methods that measure CTF require special substrates and fluorescent microscopy, rendering them less suitable in a clinical setting. Here, we demonstrate a rapid and direct approach to measure the combined CTF of a large cell population using thin polydimethylsiloxane (PDMS) cantilevers...
November 4, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29093447/e-cadherin-integrates-mechanotransduction-and-egfr-signaling-to-control-junctional-tissue-polarization-and-tight-junction-positioning
#7
Matthias Rübsam, Aaron F Mertz, Akiharu Kubo, Susanna Marg, Christian Jüngst, Gladiola Goranci-Buzhala, Astrid C Schauss, Valerie Horsley, Eric R Dufresne, Markus Moser, Wolfgang Ziegler, Masayuki Amagai, Sara A Wickström, Carien M Niessen
Generation of a barrier in multi-layered epithelia like the epidermis requires restricted positioning of functional tight junctions (TJ) to the most suprabasal viable layer. This positioning necessitates tissue-level polarization of junctions and the cytoskeleton through unknown mechanisms. Using quantitative whole-mount imaging, genetic ablation, and traction force microscopy and atomic force microscopy, we find that ubiquitously localized E-cadherin coordinates tissue polarization of tension-bearing adherens junction (AJ) and F-actin organization to allow formation of an apical TJ network only in the uppermost viable layer...
November 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28982683/mechanical-and-signaling-roles-for-keratin-intermediate-filaments-in-the-assembly-and-morphogenesis-of-mesendoderm-tissue-at-gastrulation
#8
Pooja R Sonavane, Chong Wang, Bette Dzamba, Gregory F Weber, Ammasi Periasamy, Douglas W DeSimone
Coordination of individual cell behaviors is a critical step in the assembly and morphogenesis of tissues. Xenopus mesendoderm cells migrate collectively along a fibronectin (FN) substrate at gastrulation but how the adhesive and mechanical forces required for these movements are generated and transmitted is unclear. Traction force microscopy (TFM) was used to establish that traction stresses are limited primarily to leading edge cells in mesendoderm explants and that these forces are balanced by intercellular stresses in follower rows...
October 5, 2017: Development
https://www.readbyqxmd.com/read/28955712/analysis-of-contractility-and-invasion-potential-of-two-canine-mammary-tumor-cell-lines
#9
Kaisa Rajakylä, Ramaswamy Krishnan, Sari Tojkander
Cancer cells are surrounded by a mechanically and biochemically distinct microenvironment that undergoes dynamic changes throughout the neoplastic progression. During this progression, some cancer cells acquire abnormal characteristics that potentiate their escape from the primary tumor site, to establish secondary tumors in distant organs. Recent studies with several human cancer cell lines have shown that the altered physical properties of tumor cells, such as their ability to apply high traction forces to the surroundings, are directly linked with their potential to invade and metastasize...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28952551/progress-in-integrative-biomaterial-systems-to-approach-three-dimensional-cell-mechanotransduction
#10
REVIEW
Ying Zhang, Kin Liao, Chuan Li, Alvin C K Lai, Ji-Jinn Foo, Vincent Chan
Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell-matrix and cell-cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials...
August 24, 2017: Bioengineering
https://www.readbyqxmd.com/read/28931598/coordinated-increase-of-nuclear-tension-and-lamin-a-with-matrix-stiffness-outcompetes-lamin-b-receptor-that-favors-soft-tissue-phenotypes
#11
Amnon Buxboim, Jerome Irianto, Joe Swift, Avathamsa Athirasala, Jae-Won Shin, Florian Rehfeldt, Dennis E Discher
Matrix stiffness that is sensed by a cell or measured by a purely physical probe reflects the intrinsic elasticity of the matrix and also how thick or thin the matrix is. Here, mesenchymal stem cells (MSCs) and their nuclei spread in response to thickness-corrected matrix microelasticity, with increases in nuclear tension and nuclear stiffness resulting from increases in myosin-II and lamin-A,C. Linearity between the widely varying projected area of a cell and its nucleus across many matrices, timescales, and myosin-II activity levels indicates a constant ratio of nucleus-to-cell volume, despite MSCs' lineage plasticity...
November 7, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28919310/cadherin-11-promotes-neural-crest-cell-spreading-by-reducing-intracellular-tension-mapping-adhesion-and-mechanics-in-neural-crest-explants-by-atomic-force-microscopy
#12
REVIEW
Carina Blaue, Jubin Kashef, Clemens M Franz
During development cranial neural crest cells (NCCs) display a striking transition from collective to single-cell migration, but the mechanisms enabling individual NCCs to separate from the neural crest tissue are still incompletely understood. In this study we have employed atomic force microscopy (AFM) to investigate potential adhesive and mechanical changes associated with the dissociation of individual cells from cohesive Xenopus NCC explants at early stages of migration. AFM-based single-cell force spectroscopy (SCFS) revealed a uniform distribution of cell-cell adhesion forces within NCC explants, including semi-detached leader cells in the process of delaminating from the explant edge...
September 15, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28915383/force-activatable-biosensor-enables-single-platelet-force-mapping-directly-by-fluorescence-imaging
#13
Yongliang Wang, Dana N LeVine, Margaret Gannon, Yuanchang Zhao, Anwesha Sarkar, Bailey Hoch, Xuefeng Wang
Integrin-transmitted cellular forces are critical for platelet adhesion, activation, aggregation and contraction during hemostasis and thrombosis. Measuring and mapping single platelet forces are desired in both research and clinical applications. Conventional force-to-strain based cell traction force microscopies have low resolution which is not ideal for cellular force mapping in small platelets. To enable platelet force mapping with submicron resolution, we developed a force-activatable biosensor named integrative tension sensor (ITS) which directly converts molecular tensions to fluorescent signals, therefore enabling cellular force mapping directly by fluorescence imaging...
September 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28836206/traction-force-measurement-using-deformable-microposts
#14
Tianfa Xie, Jamar Hawkins, Yubing Sun
Recent findings suggest that mechanical forces strongly influence wound repair and fibrosis across multiple organ systems. Traction force is vital to the characterization of cellular responses to mechanical stimuli. Using hydrogel-based traction force microscopy, a FRET-based tension sensor, or microengineered cantilevers, the magnitude of traction forces can be measured. Here, we describe a traction force measurement methodology using a dense array of elastomeric microposts. This platform can be used to measure the traction force of a single cell or a colony of cells with or without geometric confinement...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28797233/full-l1-regularized-traction-force-microscopy-over-whole-cells
#15
Alejandro Suñé-Auñón, Alvaro Jorge-Peñas, Rocío Aguilar-Cuenca, Miguel Vicente-Manzanares, Hans Van Oosterwyck, Arrate Muñoz-Barrutia
BACKGROUND: Traction Force Microscopy (TFM) is a widespread technique to estimate the tractions that cells exert on the surrounding substrate. To recover the tractions, it is necessary to solve an inverse problem, which is ill-posed and needs regularization to make the solution stable. The typical regularization scheme is given by the minimization of a cost functional, which is divided in two terms: the error present in the data or data fidelity term; and the regularization or penalty term...
August 10, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28793219/stiff-substrates-increase-inflammation-induced-endothelial-monolayer-tension-and-permeability
#16
Rebecca Lownes Urbano, Christina Furia, Sarah Basehore, Alisa Morss Clyne
Arterial stiffness and inflammation are associated with atherosclerosis, and each have individually been shown to increase endothelial monolayer tension and permeability. The objective of this study was to determine if substrate stiffness enhanced endothelial monolayer tension and permeability in response to inflammatory cytokines. Porcine aortic endothelial cells were cultured at confluence on polyacrylamide gels of varying stiffness and treated with either tumor necrosis factor-α (TNFα) or thrombin. Monolayer tension was measured through vinculin localization at the cell membrane, traction force microscopy, and phosphorylated myosin light chain quantity and actin fiber colocalization...
August 8, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28753694/endogenous-sheet-averaged-tension-within-a-large-epithelial-cell-colony
#17
Sandeep P Dumbali, Lanju Mei, Shizhi Qian, Venkat Maruthamuthu
Epithelial cells form quasi-two-dimensional sheets that function as contractile media to effect tissue shape changes during development and homeostasis. Endogenously generated intrasheet tension is a driver of such changes, but has predominantly been measured in the presence of directional migration. The nature of epithelial cell-generated forces transmitted over supracellular distances, in the absence of directional migration, is thus largely unclear. In this report, we consider large epithelial cell colonies which are archetypical multicell collectives with extensive cell-cell contacts but with a symmetric (circular) boundary...
October 1, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28726917/engineering-micromyocardium-to-delineate-cellular-and-extracellular-regulation-of-myocardial-tissue-contractility
#18
Nethika R Ariyasinghe, Caitlin H Reck, Alyssa A Viscio, Andrew P Petersen, Davi M Lyra-Leite, Nathan Cho, Megan L McCain
Cardiovascular diseases are a leading cause of death, in part due to limitations of existing models of the myocardium. Myocardium consists of aligned, contractile cardiac myocytes interspersed with fibroblasts that synthesize extracellular matrix (ECM). The cellular demographics and biochemical and mechanical properties of the ECM remodel in many different cardiac diseases. However, the impact of diverse cellular and extracellular remodeling on the contractile output of the myocardium are poorly understood...
September 18, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28708434/epithelial-cells-induce-a-cyclooxygenase-1-dependent-endogenous-reduction-in-airway-smooth-muscle-contractile-phenotype
#19
Michael J O'Sullivan, Elizabeth Gabriel, Alice Panariti, Chan Y Park, Gijs Ijpma, Jeffrey J Fredberg, Anne-Marie Lauzon, James G Martin
Airway smooth muscle (ASM) cells are phenotypically regulated to exist in either a proliferative or a contractile state. However the influence of other airway structural cell types on ASM cell phenotype is largely unknown. Although epithelial cells are known to drive ASM proliferation, their effects on the contractile phenotype are uncertain. In the current study we tested the hypothesis that epithelial cells reduce the contractile phenotype of ASM cells. To do so, we measured force production by traction microscopy, gene and protein expression as well as calcium release by Fura-2 ratiometric imaging...
July 14, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28674019/changes-in-e-cadherin-rigidity-sensing-regulate-cell-adhesion
#20
Caitlin Collins, Aleksandra K Denisin, Beth L Pruitt, W James Nelson
Mechanical cues are sensed and transduced by cell adhesion complexes to regulate diverse cell behaviors. Extracellular matrix (ECM) rigidity sensing by integrin adhesions has been well studied, but rigidity sensing by cadherins during cell adhesion is largely unexplored. Using mechanically tunable polyacrylamide (PA) gels functionalized with the extracellular domain of E-cadherin (Ecad-Fc), we showed that E-cadherin-dependent epithelial cell adhesion was sensitive to changes in PA gel elastic modulus that produced striking differences in cell morphology, actin organization, and membrane dynamics...
July 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
keyword
keyword
110181
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"