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traction force microscopy

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https://www.readbyqxmd.com/read/29742412/traction-force-screening-enabled-by-compliant-pdms-elastomers
#1
Haruka Yoshie, Newsha Koushki, Rosa Kaviani, Mohammad Tabatabaei, Kavitha Rajendran, Quynh Dang, Amjad Husain, Sean Yao, Chuck Li, John K Sullivan, Magali Saint-Geniez, Ramaswamy Krishnan, Allen J Ehrlicher
Actomyosin contractility is an essential element of many aspects of cellular biology and manifests as traction forces that cells exert on their surroundings. The central role of these forces makes them a novel principal therapeutic target in diverse diseases. This requires accurate and higher-capacity measurements of traction forces; however, existing methods are largely low throughput, limiting their utility in broader applications. To address this need, we employ Fourier-transform traction force microscopy in a parallelized 96-well format, which we refer to as contractile force screening...
May 8, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29740072/the-mechanical-microenvironment-regulates-ovarian-cancer-cell-morphology-migration-and-spheroid-disaggregation
#2
Andrew J McKenzie, Stephanie R Hicks, Kathryn V Svec, Hannah Naughton, Zöe L Edmunds, Alan K Howe
There is growing appreciation of the importance of the mechanical properties of the tumor microenvironment on disease progression. However, the role of extracellular matrix (ECM) stiffness and cellular mechanotransduction in epithelial ovarian cancer (EOC) is largely unknown. Here, we investigated the effect of substrate rigidity on various aspects of SKOV3 human EOC cell morphology and migration. Young's modulus values of normal mouse peritoneum, a principal target tissue for EOC metastasis, were determined by atomic force microscopy (AFM) and hydrogels were fabricated to mimic these values...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29712747/holding-tight-on-feathers-structural-specializations-and-attachment-properties-of-the-avian-ectoparasite-crataerina-pallida-diptera-hippoboscidae
#3
Dennis S Petersen, Nils Kreuter, Lars Heepe, Sebastian Büsse, Arndt H J Wellbrock, Klaudia Witte, Stanislav N Gorb
The louse fly Crataerina pallida is an obligate blood-sucking ecto-parasite of the common swift Apus apus Due to reduction of the wings, C. pallida is unable to fly, thus an effective and reliable attachment to their host's plumage is of outmost importance. Its attachment system shows several modifications in comparison to other calyptrate flies. The most prominent ones are the large tridentate claws and the dichotomously shaped setae located on the pulvilli. Based on data from morphological analysis, confocal laser scanning microscopy, cryo-scanning electron microscopy and traction force experiments, performed on native (feathers) as well as artificial substrates (glass, epoxy-resin and silicone rubber), we showed that the entire attachment system is highly adapted to the fly's lifestyle as an ectoparasite...
April 30, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29627503/local-traction-force-in-the-proximal-leading-process-triggers-nuclear-translocation-during-neuronal-migration
#4
Hiroki Umeshima, Ken-Ichi Nomura, Shuhei Yoshikawa, Marcel Hörning, Motomu Tanaka, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Mineko Kengaku
Somal translocation in long bipolar neurons is regulated by actomyosin contractile forces, yet the precise spatiotemporal sites of force generation are unknown. Here we investigate the force dynamics generated during somal translocation using traction force microscopy. Neurons with a short leading process generated a traction force in the growth cone and counteracting forces in the leading and trailing processes. In contrast, neurons with a long leading process generated a force dipole with opposing traction forces in the proximal leading process during nuclear translocation...
April 5, 2018: Neuroscience Research
https://www.readbyqxmd.com/read/29617427/investigation-of-human-ipsc-derived-cardiac-myocyte-functional-maturation-by-single-cell-traction-force-microscopy
#5
Matthew Wheelwright, Zaw Win, Jennifer L Mikkila, Kamilah Y Amen, Patrick W Alford, Joseph M Metzger
Recent advances have made it possible to readily derive cardiac myocytes from human induced pluripotent stem cells (hiPSC-CMs). HiPSC-CMs represent a valuable new experimental model for studying human cardiac muscle physiology and disease. Many laboratories have devoted substantial effort to examining the functional properties of isolated hiPSC-CMs, but to date, force production has not been adequately characterized. Here, we utilized traction force microscopy (TFM) with micro-patterning cell printing to investigate the maximum force production of isolated single hiPSC-CMs under varied culture and assay conditions...
2018: PloS One
https://www.readbyqxmd.com/read/29590169/traction-force-microscopy-of-engineered-cardiac-tissues
#6
Francesco Silvio Pasqualini, Ashutosh Agarwal, Blakely Bussie O'Connor, Qihan Liu, Sean P Sheehy, Kevin Kit Parker
Cardiac tissue development and pathology have been shown to depend sensitively on microenvironmental mechanical factors, such as extracellular matrix stiffness, in both in vivo and in vitro systems. We present a novel quantitative approach to assess cardiac structure and function by extending the classical traction force microscopy technique to tissue-level preparations. Using this system, we investigated the relationship between contractile proficiency and metabolism in neonate rat ventricular myocytes (NRVM) cultured on gels with stiffness mimicking soft immature (1 kPa), normal healthy (13 kPa), and stiff diseased (90 kPa) cardiac microenvironments...
2018: PloS One
https://www.readbyqxmd.com/read/29572404/similar-biophysical-abnormalities-in-glomeruli-and-podocytes-from-two-distinct-models
#7
Addie E Embry, Zhenan Liu, Joel M Henderson, F Jefferson Byfield, Liping Liu, Joonho Yoon, Zhenzhen Wu, Katrina Cruz, Sara Moradi, C Barton Gillombardo, Rihanna Z Hussain, Richard Doelger, Olaf Stuve, Audrey N Chang, Paul A Janmey, Leslie A Bruggeman, R Tyler Miller
Background FSGS is a pattern of podocyte injury that leads to loss of glomerular function. Podocytes support other podocytes and glomerular capillary structure, oppose hemodynamic forces, form the slit diaphragm, and have mechanical properties that permit these functions. However, the biophysical characteristics of glomeruli and podocytes in disease remain unclear. Methods Using microindentation, atomic force microscopy, immunofluorescence microscopy, quantitative RT-PCR, and a three-dimensional collagen gel contraction assay, we studied the biophysical and structural properties of glomeruli and podocytes in chronic (Tg26 mice [HIV protein expression]) and acute (protamine administration [cytoskeletal rearrangement]) models of podocyte injury...
May 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29518807/in-vitro-study-of-bonding-strength-of-zirconia-on-dentin-using-different-adhesive-systems
#8
Hind Larabi, Sibel Cetik, Hoang Thai Ha, Ramin Atash
PURPOSE: To compare different dental bonding systems that are currently available on the market. MATERIALS AND METHODS: A total of 100 extracted, intact third molars were coated in resin, cut, and divided into 10 groups of 10 molars each. Molars were bonded to zirconia blocks with a different bonding system per group. Resistance to shear forces was tested using a universal traction machine. Samples were observed under scanning electron microscopy (SEM) to determine the type of fracture...
March 2018: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/29494578/inverse-tissue-mechanics-of-cell-monolayer-expansion
#9
Yohei Kondo, Kazuhiro Aoki, Shin Ishii
Living tissues undergo deformation during morphogenesis. In this process, cells generate mechanical forces that drive the coordinated cell motion and shape changes. Recent advances in experimental and theoretical techniques have enabled in situ measurement of the mechanical forces, but the characterization of mechanical properties that determine how these forces quantitatively affect tissue deformation remains challenging, and this represents a major obstacle for the complete understanding of morphogenesis...
March 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29470990/lis1-dysfunction-leads-to-traction-force-reduction-and-cytoskeletal-disorganization-during-cell-migration
#10
Guo-Wei Jheng, Sung Sik Hur, Chia-Ming Chang, Chun-Chieh Wu, Jia-Shing Cheng, Hsiao-Hui Lee, Bon-Chu Chung, Yang-Kao Wang, Keng-Hui Lin, Juan C Del Álamo, Shu Chien, Jin-Wu Tsai
Cell migration is a critical process during development, tissue repair, and cancer metastasis. It requires complex processes of cell adhesion, cytoskeletal dynamics, and force generation. Lis1 plays an important role in the migration of neurons, fibroblasts and other cell types, and is essential for normal development of the cerebral cortex. Mutations in human LIS1 gene cause classical lissencephaly (smooth brain), resulting from defects in neuronal migration. However, how Lis1 may affect force generation in migrating cells is still not fully understood...
March 11, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29469613/aging-and-anatomical-variations-in-lung-tissue-stiffness
#11
Delphine Sicard, Andrew J Haak, Kyoung Moo Choi, Alexandria R Craig, Laura E Fredenburgh, Daniel J Tschumperlin
Lung function is inherently mechanical in nature, and depends on the capacity to conduct air and blood to and from the gas exchange regions. Variations in the elastic properties of the human lung across anatomical compartments and with aging are likely important determinants of lung function, but remain relatively poorly characterized. Here we applied atomic force microscopy to characterize human lung tissue from subjects ranging in age from 11 to 60 years old. We observed striking anatomical variations in elastic modulus, with the airways (200-350 µm diameter) the stiffest and the parenchymal regions the most compliant...
February 22, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29445207/holographic-traction-force-microscopy
#12
Stanislaw Makarchuk, Nicolas Beyer, Christian Gaiddon, Wilfried Grange, Pascal Hébraud
Traction Force Microscopy (TFM) computes the forces exerted at the surface of an elastic material by measuring induced deformations in volume. It is used to determine the pattern of the adhesion forces exerted by cells or by cellular assemblies grown onto a soft deformable substrate. Typically, colloidal particles are dispersed in the substrate and their displacement is monitored by fluorescent microscopy. As with any other fluorescent techniques, the accuracy in measuring a particule's position is ultimately limited by the number of evaluated fluorescent photons...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29404724/eigenstrain-as-a-mechanical-set-point-of-cells
#13
Shengmao Lin, Marsha C Lampi, Cynthia A Reinhart-King, Gary Tsui, Jian Wang, Carl A Nelson, Linxia Gu
Cell contraction regulates how cells sense their mechanical environment. We sought to identify the set-point of cell contraction, also referred to as tensional homeostasis. In this work, bovine aortic endothelial cells (BAECs), cultured on substrates with different stiffness, were characterized using traction force microscopy (TFM). Numerical models were developed to provide insights into the mechanics of cell-substrate interactions. Cell contraction was modeled as eigenstrain which could induce isometric cell contraction without external forces...
February 5, 2018: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/29345194/transient-stretch-induces-cytoskeletal-fluidization-through-the-severing-action-of-cofilin
#14
Bo Lan, Ramaswamy Krishnan, Chan Y Park, Rodrigo Watanabe, Ronald Panganiban, James P Butler, Quan Lu, William C Cole, Jeffrey J Fredberg
With every deep inspiration (DI) or sigh the airway wall stretches, as do the airway smooth muscle cells that it contains. In response, the airway smooth muscle (ASM) cell undergoes rapid stretch-induced cytoskeletal fluidization. As a molecular mechanism underlying that ASM fluidization response, here we demonstrate a key role for the actin-severing protein, cofilin. Using primary human ASM cells, we simulated a DI by imposing a transient stretch of physiologic magnitude and duration. We measured the resulting changes in contractile forces using traction microscopy...
January 18, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29316444/force-generation-via-%C3%AE-cardiac-myosin-titin-and-%C3%AE-actinin-drives-cardiac-sarcomere-assembly-from-cell-matrix-adhesions
#15
Anant Chopra, Matthew L Kutys, Kehan Zhang, William J Polacheck, Calvin C Sheng, Rebeccah J Luu, Jeroen Eyckmans, J Travis Hinson, Jonathan G Seidman, Christine E Seidman, Christopher S Chen
Truncating mutations in the sarcomere protein titin cause dilated cardiomyopathy due to sarcomere insufficiency. However, it remains mechanistically unclear how these mutations decrease sarcomere content in cardiomyocytes. Utilizing human induced pluripotent stem cell-derived cardiomyocytes, CRISPR/Cas9, and live microscopy, we characterize the fundamental mechanisms of human cardiac sarcomere formation. We observe that sarcomerogenesis initiates at protocostameres, sites of cell-extracellular matrix adhesion, where nucleation and centripetal assembly of α-actinin-2-containing fibers provide a template for the fusion of Z-disk precursors, Z bodies, and subsequent striation...
January 8, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29315860/nanoparticle-cell-interaction-a-cell-mechanics-perspective
#16
REVIEW
Dedy Septiadi, Federica Crippa, Thomas Lee Moore, Barbara Rothen-Rutishauser, Alke Petri-Fink
Progress in the field of nanoparticles has enabled the rapid development of multiple products and technologies; however, some nanoparticles can pose both a threat to the environment and human health. To enable their safe implementation, a comprehensive knowledge of nanoparticles and their biological interactions is needed. In vitro and in vivo toxicity tests have been considered the gold standard to evaluate nanoparticle safety, but it is becoming necessary to understand the impact of nanosystems on cell mechanics...
January 9, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29282212/musclemotion-a-versatile-open-software-tool-to-quantify-cardiomyocyte-and-cardiac-muscle-contraction-in-vitro-and-in-vivo
#17
REVIEW
Luca Sala, Berend J van Meer, Leon G J Tertoolen, Jeroen Bakkers, Milena Bellin, Richard P Davis, Chris Denning, Michel A E Dieben, Thomas Eschenhagen, Elisa Giacomelli, Catarina Grandela, Arne Hansen, Eduard R Holman, Monique R M Jongbloed, Sarah M Kamel, Charlotte D Koopman, Quentin Lachaud, Ingra Mannhardt, Mervyn P H Mol, Diogo Mosqueira, Valeria V Orlova, Robert Passier, Marcelo C Ribeiro, Umber Saleem, Godfrey L Smith, Francis L Burton, Christine L Mummery
RATIONALE: There are several methods to measure cardiomyocyte and muscle contraction, but these require customized hardware, expensive apparatus, and advanced informatics or can only be used in single experimental models. Consequently, data and techniques have been difficult to reproduce across models and laboratories, analysis is time consuming, and only specialist researchers can quantify data. OBJECTIVE: Here, we describe and validate an automated, open-source software tool (MUSCLEMOTION) adaptable for use with standard laboratory and clinical imaging equipment that enables quantitative analysis of normal cardiac contraction, disease phenotypes, and pharmacological responses...
February 2, 2018: Circulation Research
https://www.readbyqxmd.com/read/29242157/determination-of-green-s-function-for-three-dimensional-traction-force-reconstruction-based-on-geometry-and-boundary-conditions-of-cell-culture-matrices
#18
Y Du, S C B Herath, Q G Wang, H Asada, P C Y Chen
Cell migration plays a particular important role in the initiation and progression of many physical processes and pathological conditions such as tumor invasion and metastasis. Three-dimensional traction force microscopy (TFM) of high resolution and high accuracy is being developed in an effort to unveil the underlying mechanical process of cell migration in a vivo-like environment. Linear elasticity-based TFM (LETM) as a mainstream approach relies on the Green's function (that relates traction forces to matrix deformation), of which the inherent boundary conditions and geometry of the matrix could remarkably affect the result as suggested by previous 2D studies...
February 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29229906/direction-of-actin-flow-dictates-integrin-lfa-1-orientation-during-leukocyte-migration
#19
Pontus Nordenfelt, Travis I Moore, Shalin B Mehta, Joseph Mathew Kalappurakkal, Vinay Swaminathan, Nobuyasu Koga, Talley J Lambert, David Baker, Jennifer C Waters, Rudolf Oldenbourg, Tomomi Tani, Satyajit Mayor, Clare M Waterman, Timothy A Springer
Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts a specific orientation dictated by the direction of actin flow on the surface of migrating cells. We insert GFP into the rigid, ligand-binding head of the integrin, model with Rosetta the orientation of GFP and its transition dipole relative to the integrin head, and measure orientation with fluorescence polarization microscopy...
December 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/29199833/single-cell-optical-distortion-correction-and-label-free-3d-cell-shape-reconstruction-on-lattices-of-nanostructures
#20
Jürgen Stephan, Felix Keber, Valentin Stierle, Joachim O Rädler, Philipp Paulitschke
Imaging techniques can be compromised by aberrations. Especially when imaging through biological specimens, sample-induced distortions can limit localization accuracy. In particular, this phenomenon affects localization microscopy, traction force measurements, and single-particle tracking, which offer high-resolution insights into biological tissue. Here we present a method for quantifying and correcting the optical distortions induced by single, adherent, living cells. The technique uses periodically patterned gold nanostructures as a reference framework to quantify optically induced displacements with micrometer-scale sampling density and an accuracy of a few nanometers...
December 13, 2017: Nano Letters
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