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https://www.readbyqxmd.com/read/29666653/mesenchymal-stem-cells-in-oriented-plga-acecm-composite-scaffolds-enhance-structure-specific-regeneration-of-hyaline-cartilage-in-a-rabbit-model
#1
Weimin Guo, Xifu Zheng, Weiguo Zhang, Mingxue Chen, Zhenyong Wang, Chunxiang Hao, Jingxiang Huang, Zhiguo Yuan, Yu Zhang, Mingjie Wang, Jiang Peng, Aiyuan Wang, Yu Wang, Xiang Sui, Wenjing Xu, Shuyun Liu, Shibi Lu, Quanyi Guo
Articular cartilage lacks a blood supply and nerves. Hence, articular cartilage regeneration remains a major challenge in orthopedics. Decellularized extracellular matrix- (ECM-) based strategies have recently received particular attention. The structure of native cartilage exhibits complex zonal heterogeneity. Specifically, the development of a tissue-engineered scaffold mimicking the aligned structure of native cartilage would be of great utility in terms of cartilage regeneration. Previously, we fabricated oriented PLGA/ACECM (natural, nanofibrous, articular cartilage ECM) composite scaffolds...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29664923/effect-of-cartilaginous-endplates-on-extruded-disc-resorption-in-lumbar-disc-herniation
#2
Kenichi Kawaguchi, Katsumi Harimaya, Yoshihiro Matsumoto, Mitsumasa Hayashida, Seiji Okada, Keiichiro Iida, Go Kato, Kuniyoshi Tsuchiya, Toshio Doi, Yoshinao Oda, Yukihide Iwamoto, Yasuharu Nakashima
OBJECTIVE: The aim of this study was to investigate the clinicopathologic features of lumbar disc herniation (LDH) with endplate degeneration and the association between cartilaginous fragments and inflammatory response to the herniated disc. SUMMARY OF BACKGROUND DATA: LDH often involves hyaline cartilage fragments pulled from the vertebral endplates. Modic changes are closely associated with LDH that contains hyaline cartilage, and cartilaginous endplates seem to affect resorption of the herniated disc...
2018: PloS One
https://www.readbyqxmd.com/read/29661266/characterization-of-polish-steinernema-silvaticum-isolates-nematoda-steinernematidae-using-morphological-and-molecular-data
#3
M Lis, E Sajnaga, A Kreft, T Skrzypek, W Kazimierczak
Four strains of entomopathogenic nematodes were isolated with a live trap method in southern Poland. The isolates were identified as Steinernema silvaticum based on morphological, morphometric and molecular data. Infective juveniles of Polish S. silvaticum isolates differ in body length from S. weiseri (951 vs 740 μm, respectively), and in the hyaline tail portion from S. kraussei (48 vs 38%, respectively). First-generation males of S. silvaticum are longer than those of S. kraussei, S. weiseri and S. ichnusae (1829 vs 1400, 1180 and 1341 μm, respectively)...
April 17, 2018: Journal of Helminthology
https://www.readbyqxmd.com/read/29658796/lichen-sclerosus-of-the-oral-mucosa-clinical-and-histopathological-findings-review-of-the-literature-and-a-case-report
#4
Anna-Maija Matela, Jaana Hagström, Hellevi Ruokonen
OBJECTIVES: Lichen sclerosus (LS) is a rare, chronic mucocutaneous disease that most frequently affects the female genital area. Oral manifestations are seldom detected; only 36 well-documented and histopathologically verified oral LS cases have been published. MATERIALS AND METHODS: Here we describe one patient affected by oral (LS) and review of the literature 1957-2017. RESULTS: Findings in our review suggest a female to male ratio of 1...
April 16, 2018: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/29658148/biochemical-and-biomechanical-characterization-of-equine-cervical-facet-joint-cartilage
#5
S A O'Leary, J L White, J C Hu, K A Athanasiou
BACKGROUND: The equine cervical facet joint is a site of significant pathology. Located bilaterally on the dorsal spine, these diarthrodial joints work in conjunction with the intervertebral disc to facilitate appropriate spinal motion. Despite the high prevalence of pathology in this joint, the facet joint is understudied and thus lacking in viable treatment options. OBJECTIVE: The goal of this study was to characterise equine facet joint cartilage and provide a comprehensive database describing the morphological, histological, biochemical, and biomechanical properties of this tissue...
April 15, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29656239/mechanical-alterations-of-the-bone-cartilage-unit-in-a-rabbit-model-of-early-osteoarthrosis
#6
Sarah Pragnère, Caroline Boulocher, Ophélie Pollet, Catherine Bosser, Aurélie Levillain, Magali Cruel, Thierry Hoc
OBJECTIVE: The purpose of this study was to assess mechanical properties along with microstructural modifications of the hyaline cartilage (HC), calcified cartilage (CC) and cortical plate (Ct.Pt), in an anterior cruciate ligament transection (ACLT) model. Medial femoral condyles of six healthy rabbits (control group) and of six ACLT rabbits 6 weeks after OA induction were explanted. The zone of interest (ZOI) for all experiments was defined as the weight bearing areas of the samples...
April 3, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29655900/the-anterolateral-ligament-is-a-distinct-ligamentous-structure-a-histological-explanation
#7
Thomas Neri, Pierre Dalcol, Fabien Palpacuer, Florian Bergandi, Jean Michel Prades, Frederic Farizon, Remi Philippot, Michel Peoc'h
BACKGROUND: The aim was to determine whether the anterolateral ligament (ALL) had a histological structure that defined it as a real ligament. METHODS: Histological examination of 30 ALL samples taken from fresh-frozen knees were performed. The ALL femoral insertion and its relationship with the lateral collateral ligament (LCL) were studied and the tibial insertion and its relationship with articular cartilage of the tibial joint surface were analyzed. For the ligamentous part, its histological structure and its differences with the articular capsule were studied...
April 11, 2018: Knee
https://www.readbyqxmd.com/read/29655415/clinicopathological-analysis-of-232-radicular-cysts-of-the-jawbone-in-a-population-of-southern-taiwanese-patients
#8
Jeng-Huey Chen, Chih-Huang Tseng, Wen-Chen Wang, Ching-Yi Chen, Fu-Hsiung Chuang, Yuk-Kwan Chen
This retrospective study aimed to evaluate the clinicopathological features of 232 cases of radicular cyst (January 2001-December 2016) submitted for histopathological examination to Department of Oral Pathology by endodontists in our institution. Demographic data including age, gender, affected site, involved tooth, and histopathological features, were reviewed. The study population comprised 133 females (57.3%) and 99 males (42.7%), with a mean age of 40.5 years and an age range of 13-78 years. Two-hundred and one cysts occurred in the maxilla (86...
April 2018: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/29652818/beneficial-effects-of-different-flavonoids-on-vascular-and-renal-function-in-l-name-hypertensive-rats
#9
M Dolores Paredes, Paola Romecín, Noemí M Atucha, Francisco O'Valle, Julián Castillo, M Clara Ortiz, Joaquín García-Estañ
BACKGROUND: we have evaluated the antihypertensive effect of several flavonoid extracts in a rat model of arterial hypertension caused by chronic administration (6 weeks) of the nitric oxide synthesis inhibitor, L-NAME. METHODS: Sprague Dawley rats received L-NAME alone or L-NAME plus flavonoid-rich vegetal extracts (Lemon, Grapefruit + Bitter Orange, and Cocoa) or purified flavonoids (Apigenin and Diosmin) for 6 weeks. RESULTS: L-NAME treatment resulted in a marked elevation of blood pressure, and treatment with Apigenin, Lemon Extract, and Grapefruit + Bitter Orange extracts significantly reduced the elevated blood pressure of these animals...
April 13, 2018: Nutrients
https://www.readbyqxmd.com/read/29651419/management-of-glioblastoma-multiforme-in-a-patient-treated-with-ketogenic-metabolic-therapy-and-modified-standard-of-care-a-24-month-follow-up
#10
Ahmed M A Elsakka, Mohamed Abdel Bary, Eman Abdelzaher, Mostafa Elnaggar, Miriam Kalamian, Purna Mukherjee, Thomas N Seyfried
Few advances have been made in overall survival for glioblastoma multiforme (GBM) in more than 40 years. Here, we report the case of a 38-year-old man who presented with chronic headache, nausea, and vomiting accompanied by left partial motor seizures and upper left limb weakness. Enhanced brain magnetic resonance imaging revealed a solid cystic lesion in the right partial space suggesting GBM. Serum testing revealed vitamin D deficiency and elevated levels of insulin and triglycerides. Prior to subtotal tumor resection and standard of care (SOC), the patient conducted a 72-h water-only fast...
2018: Frontiers in Nutrition
https://www.readbyqxmd.com/read/29642550/healing-of-osteochondral-defects-implanted-with-biomimetic-scaffolds-of-poly-%C3%AE%C2%B5-caprolactone-hydroxyapatite-and-glycidyl-methacrylate-modified-hyaluronic-acid-in-a-minipig
#11
Yi-Ho Hsieh, Bo-Yuan Shen, Yao-Horng Wang, Bojain Lin, Hung-Maan Lee, Ming-Fa Hsieh
Articular cartilage is a structure lack of vascular distribution. Once the cartilage is injured or diseased, it is unable to regenerate by itself. Surgical treatments do not effectively heal defects in articular cartilage. Tissue engineering is the most potential solution to this problem. In this study, methoxy poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL) and hydroxyapatite at a weight ratio of 2:1 were mixed via fused deposition modeling (FDM) layer by layer to form a solid scaffold. The scaffolds were further infiltrated with glycidyl methacrylate hyaluronic acid loading with 10 ng/mL of Transforming Growth Factor-β1 and photo cross-linked on top of the scaffolds...
April 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29629804/quantitative-assessment-of-degenerative-cartilage-and-subchondral-bony-lesions-in-a-preserved-cadaveric-knee-propagation-based-phase-contrast-ct-versus-conventional-mri-and-ct
#12
Tobias Geith, Emmanuel Brun, Alberto Mittone, Sergei Gasilov, Loriane Weber, Silvia Adam-Neumair, Alberto Bravin, Maximilian Reiser, Paola Coan, Annie Horng
OBJECTIVE: The aim of this study was to quantitatively assess hyaline cartilage and subchondral bone conditions in a fully preserved cadaveric human knee joint using high-resolution x-ray propagation-based phase-contrast imaging (PBI) CT and to compare the performance of the new technique with conventional CT and MRI. MATERIALS AND METHODS: A cadaveric human knee was examined using an x-ray beam of 60 keV, a detector with a 90-mm2 FOV, and a pixel size of 46 × 46 μm2 ...
April 9, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29626596/renal-histology-in-a-patient-with-tafro-syndrome-a-case-report
#13
Hiroki Mizuno, Akinari Sekine, Masahiko Oguro, Yoichi Oshima, Masahiro Kawada, Keiichi Sumida, Masayuki Yamanouchi, Noriko Hayami, Tatsuya Suwabe, Rikako Hiramatsu, Eiko Hasegawa, Junichi Hoshino, Naoki Sawa, Takashi Fujii, Kenmei Takaichi, Kenichi Ohashi, Yoshifumi Ubara
An 84-year-old Japanese man was admitted due to anasarca, thrombocytopenia, systemic inflammation, and progressive renal insufficiency, resistant to diuretics, glucocorticoid therapy and plasma exchange. Renal biopsy showed diffuse endocapillary proliferation and mesangiolysis without any immune deposits. Tocilizumab suppressed systemic inflammation, resulting in improvement of anasarca and renal dysfunction, but thrombocytopenia persisted and platelet-associated IgG antibody was elevated. Though romiplostim was effective for thrombocytopenia, the patient died of aspiration pneumonia after cerebral hemorrhage...
April 4, 2018: Human Pathology
https://www.readbyqxmd.com/read/29624002/expression-of-adamts-7-in-myocardial-dystrophy-associated-with-white-muscle-disease-in-lambs
#14
N Yumusak, A Yigin, P F Polat, M Hitit, R Yilmaz
The aim of the present study was to investigate the role of ADAMTS-7 gene in the pathogenesis of myocardial dystrophy associated with white muscle disease (WMD) in lambs. A total of 217 cardiac tissue samples from lambs with WMD were used in the study. Histopathological sections of the samples were stained with hematoxylin-eosin (HE) and examined using Western-blot, real-time PCR (RT-PCR) and immunohistochemistry for ADAMTS-7 gene expression, and the findings were statistically evaluated. Histopathological examinations revealed fibrosis associated with hyalinization, necrosis and granular calcifications in cardiomyocytes...
March 2018: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29623744/endometrial-stromal-sarcoma-with-hyalinizing-giant-rosettes-mimicking-low-grade-fibromyxoid-sarcoma
#15
Eva Kolson Kokohaare, Dirk C Strauss, Robin L Jones, Khin Thway
We highlight a rare variant pattern of low-grade endometrial stromal sarcoma showing extensive collagenous rosette formation, closely mimicking low-grade fibromyxoid sarcoma. Additionally, this neoplasm showed diffuse and strong expression of muscle markers, favoring an initial diagnosis of leiomyosarcoma. Reverse transcription-polymerase chain reaction showed the presence of JAZF1-SUZ12 fusion transcripts, and this highlights the broad morphologic and immunophenotypic spectrum of endometrial stromal sarcoma...
April 1, 2018: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29621303/time-dependent-regulation-of-morphological-changes-and-cartilage-differentiation-markers-in-the-mouse-pubic-symphysis-during-pregnancy-and-postpartum-recovery
#16
Bianca Gazieri Castelucci, Sílvio Roberto Consonni, Viviane Souza Rosa, Lucimara Aparecida Sensiate, Paula Cristina Rugno Delatti, Lúcia Elvira Alvares, Paulo Pinto Joazeiro
Animal models commonly serve as a bridge between in vitro experiments and clinical applications; however, few physiological processes in adult animals are sufficient to serve as proof-of-concept models for cartilage regeneration. Intriguingly, some rodents, such as young adult mice, undergo physiological connective tissue modifications to birth canal elements such as the pubic symphysis during pregnancy; therefore, we investigated whether the differential expression of cartilage differentiation markers is associated with cartilaginous tissue morphological modifications during these changes...
2018: PloS One
https://www.readbyqxmd.com/read/29610697/a-case-of-spondyloarthritis-in-patient-affected-by-unicentric-castleman-s-disease-effectively-managed-with-surgery-resection-and-tocilizumab-treatment
#17
M Filippini, S Cartella, O Bonzanini, E Morello, A Tincani
A 38-year-old woman was referred to our hospital for rheumatologic manifestations (migrant arthritis and tenosynovitis), without psoriasis or family history of psoriasis, gastroenteric manifestations, or recent genitourinary infections. The instrumental and laboratory tests have suggested a diagnosis of undifferentiated seronegative HLA-B27-positive spondyloarthritis with predominantly peripheral involvement. The symptoms were very severe and resistant to anti-inflammatory drugs and steroids. She had a history of hyaline-vascular unicentric Castleman's disease (HBV, HIV, and HHV-8 negative) treated with surgery resection...
2018: Case Reports in Rheumatology
https://www.readbyqxmd.com/read/29608410/armillaria-mexicana-a-newly-described-species-from-mexico
#18
Rubén Damián Elías-Román, Rosario Medel-Ortiz, Dionicio Alvarado-Rosales, John W Hanna, Amy L Ross-Davis, Mee-Sook Kim, Ned B Klopfenstein
Armillaria mexicana (Agaricales, Physalacriaceae) is described as a new species based on morphology, DNA sequence data, and phylogenetic analyses. It clearly differs from previously reported Armillaria species in North, Central, and South America. It is characterized by the absence of fibulae in the basidioma, abundant cheilocystidia, and ellipsoidal, hyaline basidiospores that are apparently smooth under light microscope, but slightly to moderately rugulose under scanning electron microscope. It is differentiated from other Armillaria species by macromorphological characters, including annulus structure, pileus and stipe coloration, and other structures...
January 19, 2018: Mycologia
https://www.readbyqxmd.com/read/29601807/utility-of-site-specific-biopsy-for-diagnosis-of-desmoplastic-malignant-mesothelioma
#19
Takashi Sakai, Kenta Tane, Yuko Usui, Tomohiro Miyoshi, Shingo Matsumoto, Keiju Aokage, Koichi Goto, Masayuki Suzuki, Genichiro Ishii, Masahiro Tsuboi
Desmoplastic malignant mesothelioma (DMM) is a relatively rare histological subtype of malignant pleural mesothelioma (MPM). Among MPMs, the diagnosis of DMM is particularly difficult because the tumor is largely composed of dense hyalinized fibrous stroma and few malignant cells. We present two cases of DMM, one in a 47-year-old man and the other in a 64-year-old man. Based on detailed pathological findings in these two cases, we conclude that cases of DMM that cannot otherwise be diagnosed using conventional pleural biopsy, may be diagnosed successfully using wedge lung resection...
March 27, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29600660/-subchondral-drilling-method-combined-with-gum-bletilla-complex-to-repair-articular-cartilage-defects
#20
Yong Huang, Xin-Ling Wang, Heng Qiu, Yi-Cheng Xiao, Zong-Hong Wu, Jian Xu
Two types(A model and B model) of articular cartilage defect models were prepared by using adult New Zealand white rabbits. A model group was applied by drilling without through subchondral bone, whose right joint was repaired by composite scaffolds made by seed cell, gum-bletilla as well as Pluronic F-127, and left side was blank control. B model group was applied by subchondral drilling method, whose right joint was repaired by using composite scaffolds made by gum-bletilla and Pluronic F-127 without seed cells, and left side was blank control...
February 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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