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https://www.readbyqxmd.com/read/29666250/developmentally-inspired-programming-of-adult-human-mesenchymal-stromal-cells-toward-stable-chondrogenesis
#1
Paola Occhetta, Sebastien Pigeot, Marco Rasponi, Boris Dasen, Arne Mehrkens, Thomas Ullrich, Ina Kramer, Sabine Guth-Gundel, Andrea Barbero, Ivan Martin
It is generally accepted that adult human bone marrow-derived mesenchymal stromal cells (hMSCs) are default committed toward osteogenesis. Even when induced to chondrogenesis, hMSCs typically form hypertrophic cartilage that undergoes endochondral ossification. Because embryonic mesenchyme is obviously competent to generate phenotypically stable cartilage, it is questioned whether there is a correspondence between mesenchymal progenitor compartments during development and in adulthood. Here we tested whether forcing specific early events of articular cartilage development can program hMSC fate toward stable chondrogenesis...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29664223/chondrogenic-response-in-presence-of-cartilage-extracellular-matrix-nanoparticles
#2
Saeed Zahiri, Elahe Masaeli, Elahe Poorazizi, Mohammad Hossein Nasr-Esfahani
Current studies based on regenerative medicine suggest, decellularized extracellular matrix (DC-ECM) components can regulate cell phenotype. In this regard, it is believed, presence of cartilage extracellular matrix particles in culture condition could produce physical and biochemical supportive cues for chondrogenesis. In this study, DC-ECM nanoparticles with average size of 61.5± 22.4 nm were produced by decellularization and mechanical processing. Homogenous distribution and nano scale size of yield particles were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) microscopy imaging...
April 17, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29663368/a-peptide-that-blocks-the-interaction-of-nf-%C3%AE%C2%BAb-p65-subunit-with-smad4-enhances-bmp2-induced-osteogenesis
#3
Mariko Urata, Shoichiro Kokabu, Takuma Matsubara, Goro Sugiyama, Chihiro Nakatomi, Hiroshi Takeuchi, Shizu Hirata-Tsuchiya, Kazuhiro Aoki, Yukihiko Tamura, Yasuko Moriyama, Yasunori Ayukawa, Miho Matsuda, Min Zhang, Kiyoshi Koyano, Chiaki Kitamura, Eijiro Jimi
Bone morphogenetic protein (BMP) potentiates bone formation through the Smad signaling pathway in vitro and in vivo. The transcription factor nuclear factor κB (NF-κB) suppresses BMP-induced osteoblast differentiation. Recently, we identified that the transactivation (TA) 2 domain of p65, a main subunit of NF-κB, interacts with the mad homology (MH) 1 domain of Smad4 to inhibit BMP signaling. Therefore, we further attempted to identify the interacting regions of these two molecules at the amino acid level...
April 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29658381/hydrogen-peroxide-a-potent-inducer-of-differentiation-of-human-adipose-derived-stem-cells-into-chondrocytes
#4
Farjam Goudarzi, Adel Mohammadalipour, Majid Bahabadi, Mohammad Taghi, Goodarzi, Arash Sarveazad, Iraj Khodadadi
Common protocols for chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) are generally expensive and time-consuming and so far, have not successfully recreated pure chondrocytes. We hypothesize that a low level of H2 O2 may induce differentiation of ADSCs into chondrocytes in a shorter incubation time and relatively lower cost. Therefore, this study aimed to comparatively investigate the effectiveness of H2 O2 -containing or free medium in the induction of ADSCs to chondrocytes. ADSCs were isolated from the lipoaspirate of four healthy females and evaluated by immunophenotyping for their CD90, CD73, CD44, CD34, and CD45 cell surface markers...
April 15, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29623134/generating-chondromimetic-mesenchymal-stem-cell-spheroids-by-regulating-media-composition-and-surface-coating
#5
BanuPriya Sridharan, Amy D Laflin, Michael S Detamore
INTRODUCTION: Spheroids of mesenchymal stem cells (MSCs) in cartilage tissue engineering have been shown to enhance regenerative potential owing to their 3D structure. In this study, we explored the possibility of priming spheroids under different media to replace the use of inductive surface coatings for chondrogenic differentiation. METHODS: Rat bone marrow-derived MSCs were organized into cell spheroids by the hanging drop technique and subsequently cultured on hyaluronic acid (HA) coated or non-coated well plates under different cell media conditions...
April 2018: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/29621359/applying-chlorogenic-acid-in-an-alginate-scaffold-of-chondrocytes-can-improve-the-repair-of-damaged-articular-cartilage
#6
Xin Cheng, Ke Li, Shengsong Xu, Peizhi Li, Yu Yan, Guang Wang, Zachary Berman, Rui Guo, Jianxin Liang, Sira Traore, Xuesong Yang
Damaged cartilage has very low regenerative potential which has led to the search for novel tissue-engineering approaches to help treat cartilage defects. While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract, chlorogenic acid (CGA), as part of chondrocyte transplantation on a model of knee joint injury in chicks. First, primary cultured chondrocytes used to evaluate the effects of CGA on chondrogenesis. Then using an articular cartilage injury model of chick knee we assessed the functional recovery after transplantation of the complexes containing chondrocytes and CGA in an alginate scaffold...
2018: PloS One
https://www.readbyqxmd.com/read/29620442/mir-21-stat3-signal-is-involved-in-odontoblast-differentiation-of-human-dental-pulp-stem-cells-mediated-by-tnf-%C3%AE
#7
Ke Xu, Jingwen Xiao, Ke Zheng, Xingmei Feng, Jinlong Zhang, Donghui Song, Chenfei Wang, Xiang Shen, Xin Zhao, Changbo Wei, Dan Huang, Guijuan Feng
Dental pulp stem cells (DPSCs), as one type of mesenchymal stem cells (MSCs), have the capability of self-renewal and multipotency to differentiate into several cell lineages, including osteogenesis, odontoblasts, chondrogenesis, neurogenesis, and adipogenesis. It has found that tumor necrosis factor-α (TNF-α) can promote osteogenic differentiation of human DPSCs in our previous studies. Other experimentation revealed that signal transducer and activator of transcription 3 (STAT3) underwent a rapid activation both in osteogenesis and inflammation microenvironment of MSCs in vitro...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29601969/concepts-and-challenges-in-the-use-of-mesenchymal-stem-cells-as-a-treatment-for-cartilage-damage-in-the-horse
#8
REVIEW
Mohammed Zayed, Steve Adair, Tena Ursini, James Schumacher, Nabil Misk, Madhu Dhar
Osteoarthritis (OA), the most common form of joint disease affecting humans and horses, is characterized by the advance and decline of cartilage and loss of function of the affected joint. The progression of OA is steadily accompanied with biochemical events, which interfere with the cytokines and proteolytic enzymes responsible for progress of the disease. Recently, regenerative therapies have been used with an assumption that mesenchymal stem cells (MSCs) possess the potential to prevent the advancement of cartilage damage and potentially regenerate the injured tissue with an ultimate goal of preventing OA...
March 20, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29582671/a-standardized-method-of-applying-toluidine-blue-metachromatic-staining-for-assessment-of-chondrogenesis
#9
Natasja Leth Bergholt, Helle Lysdahl, Martin Lind, Casper Bindzus Foldager
Objectives Staining with toluidine blue is a well-established procedure for the histological assessment of cartilaginous- and chondrogenic-differentiated tissues. Being a cationic dye, toluidine blue staining visualizes proteoglycans in a tissue because of its high affinity for the sulfate groups in proteoglycans. It is generally accepted that metachromatic staining with toluidine blue represents cartilaginous matrix and that the degree of positive staining corresponds with the amount of proteoglycans. Design Articular cartilage and pellets of chondrocytes or bone marrow stromal cells were analyzed with a standardized staining procedure for toluidine blue...
March 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29582166/restoring-the-il-1%C3%AE-nf-%C3%AE%C2%BAb-induced-impaired-chondrogenesis-by-diallyl-disulfide-in-human-adipose-derived-mesenchymal-stem-cells-via-attenuation-of-reactive-oxygen-species-and-elevation-of-antioxidant-enzymes
#10
Kobra Bahrampour Juybari, Tunku Kamarul, Mohammad Najafi, Davood Jafari, Ali Mohammad Sharifi
Strategies based on mesenchymal stem cell (MSC) therapy for restoring injured articular cartilage are not effective enough in osteoarthritis (OA). Due to the enhanced inflammation and oxidative stress in OA microenvironment, differentiation of MSCs into chondrocytes would be impaired. This study aims to explore the effects of diallyl disulfide (DADS) on IL-1β-mediated inflammation and oxidative stress in human adipose derived mesenchymal stem cells (hADSCs) during chondrogenesis. MTT assay was employed to examine the effects of various concentrations of DADS on the viability of hADSCs at different time scales to obtain non-cytotoxic concentration range of DADS...
March 26, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29577904/giantin-is-required-for-coordinated-production-of-aggrecan-link-protein-and-type-xi-collagen-during-chondrogenesis
#11
Kentaro Katayama, Mao Kuriki, Tomoyo Kamiya, Yuki Tochigi, Hiroetsu Suzuki
Extracellular matrix (ECM) constitutes a proper micro-environment for cell proliferation, migration and differentiation, as well as playing pivotal roles in developmental processes including endochondral ossification. Cartilage ECM is mainly composed of fibrous proteins, including collagen, proteoglycan, and hyaluronan. Because almost all ECM components are transported by intracellular vesicular transport systems, molecules that mediate vesicle transport are also important for endochondral ossification. Giantin, encoded by the Golgb1 gene, is a tethering factor for coatomer 1 (COPI) vesicles and functions in the cis-medial Golgi compartments...
March 22, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29577606/a-mmp7-sensitive-photoclickable-biomimetic-hydrogel-for-msc-encapsulation-towards-engineering-human-cartilage
#12
Elizabeth A Aisenbrey, Stephanie J Bryant
Cartilage tissue engineering strategies that use in situ forming degradable hydrogels for mesenchymal stem cell (MSC) delivery are promising for treating chondral defects. Hydrogels that recapitulate aspects of the native tissue have the potential to encourage chondrogenesis, permit cellular mediated degradation, and facilitate tissue growth. This study investigated photoclickable poly(ethylene glycol) (PEG) hydrogels, which were tailored to mimic the cartilage microenvironment by incorporating extracellular matrix analogs, chondroitin sulfate and RGD, and crosslinks sensitive to matrix metalloproteinase 7 (MMP7)...
March 25, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29577017/high-density-human-mesenchymal-stem-cell-rings-with-spatiotemporally-controlled-morphogen-presentation-as-building-blocks-for-engineering-bone-diaphyseal-tissue
#13
Samuel Herberg, Daniel Varghai, Yuxuan Cheng, Anna D Dikina, Phuong N Dang, Marsha W Rolle, Eben Alsberg
Emerging biomimetic tissue engineering strategies aim to partially recapitulate fundamental events that transpire during embryonic skeletal development; namely, cellular self-organization and targeted morphogenetic pathway activation. Here, we describe self-assembled, scaffold-free human mesenchymal stem cell (hMSC) rings featuring microparticle-mediated presentation of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2). We tested the hypothesis that spatiotemporally-controlled dual presentation of TGF-β1 and BMP-2 is superior in modulating in vitro endochondral ossification of high-density cellular constructs compared to single morphogen delivery...
2018: Nanotheranostics
https://www.readbyqxmd.com/read/29575980/atrazine-affects-craniofacial-chondrogenesis-and-axial-skeleton-mineralization-in-zebrafish-danio-rerio
#14
Benjamin S Walker, Alexander G Kramer, Christopher S Lassiter
Atrazine is a commonly used herbicide that has previously been implicated as an endocrine-disrupting compound. Previous studies have shown that estrogenic endocrine-disrupting compounds affect the development of the heart, cartilage, and bone in zebrafish ( Danio rerio). To determine whether atrazine has effects similar to other endocrine disruptors, zebrafish embryos were treated with a range of atrazine concentrations. Atrazine treatment at a low concentration of 0.1 µM resulted in significant differences in craniofacial cartilage elements, while concentrations ≥1 µM led to decreased survival and increased heart rates...
January 1, 2018: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/29565479/morphologic-and-molecular-alteration-during-tibia-fracture-healing-in-rat
#15
M-D Yu, B-H Su, X-X Zhang
OBJECTIVE: To monitor morphological feature and related osteogenic and bone metabolic change during healing of tibia fracture in a rat model. MATERIALS AND METHODS: Tibia density and trabecular thickness were evaluated. Histopathology was examined by HE staining. Serous inflammatory factors IL-4, IL-6, TNF-α and metabolic biomarkers ALP, β-CTX, P1NP, were determined by ELISA. The expression of RUNX2, TGF-β1, VEGF-α, BMP-2, BMP-4, and BMP-7 in callus tissue were qualified by RT-PCR...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29564424/intra-hydrogel-culture-prevents-transformation-of-mesenchymal-stem-cells-induced-by-monolayer-expansion
#16
Tongmeng Jiang, Junting Liu, Yiqiang Ouyang, Huayu Wu, Li Zheng, Jinmin Zhao, Xingdong Zhang
In this study, we report that the intra-hydrogel culture system mitigates the transformation of mesenchymal stem cells (MSCs) induced by two-dimensional (2D) expansion. MSCs expanded in monolayer culture prior to encapsulation in collagen hydrogels (group eMSCs-CH) featured impaired stemness in chondrogenesis, comparing with the freshly isolated bone marrow mononuclear cells seeded directly in collagen hydrogels (group fMSCs-CH). The molecular mechanism of the in vitro expansion-triggered damage to MSCs was detected through genome-wide microarray analysis...
March 22, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29556415/the-effect-of-serum-types-on-chondrogenic-differentiation-of-adipose-derived-stem-cells
#17
Hyeran Cho, Aeri Lee, Kyobum Kim
Background: Fetal bovine serum (FBS) is the most essential supplement in culture media for cellular proliferation, metabolism, and differentiation. However, due to a limited supply and subsequently rising prices, a series of studies have investigated a biological feasibility of replaceable serums to substitute FBS. Along with the increasing interests to manufacture stem cell-based cellular products, optimizing the composition of culture media including serums and exogenous growth factors (GFs) is of importance...
2018: Biomaterials Research
https://www.readbyqxmd.com/read/29550827/mir-125b-inhibits-lps-induced-inflammatory-injury-via-targeting-mip-1%C3%AE-in-chondrogenic-cell-atdc5
#18
Jinling Jia, Jingyu Wang, Junlei Zhang, Mingxing Cui, Xiaohui Sun, Qingjiang Li, Bin Zhao
BACKGROUND/AIMS: Chondrocyte apoptosis is largely responsible for cartilage degeneration in osteoarthritis (OA). MicroRNAs (miRNAs) play an important role in chondrogenesis and cartilage remodeling. This study explored the effect of miR-125b on inflammatory injury in chondrogenic cells. METHODS: LPS was used to simulate inflammatory injury in murine chondrogenic ATDC5 cell lines. Targeting effect of miR-125b on MIP-1α 3'UTR was assessed by dual luciferase activity assay...
March 13, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29536127/outcomes-of-cartilage-repair-techniques-for-chondral-injury-in-the-hip-a-systematic-review
#19
REVIEW
Naoki Nakano, Chetan Gohal, Andrew Duong, Olufemi R Ayeni, Vikas Khanduja
OBJECTIVE/PURPOSE: The aim of the study was to assess the options of treatment and their related outcomes for chondral injuries in the hip based on the available evidence whilst highlighting new and innovative techniques. METHODS: A systematic review of the literature from PubMed (Medline), EMBASE, Google Scholar, British Nursing Index (BNI), Cumulative Index to Nursing and Allied Health Literature (CINAHL) and Allied and Complementary Medicine Database (AMED) was undertaken from their inception to March 2017 using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines...
March 13, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29535785/different-chondrogenic-potential-among-human-induced-pluripotent-stem-cells-from-diverse-origin-primary-cells
#20
Yeri Alice Rim, Yoojun Nam, Narae Park, Hyerin Jung, Yeonsue Jang, Jennifer Lee, Ji Hyeon Ju
Scientists have tried to reprogram various origins of primary cells into human induced pluripotent stem cells (hiPSCs). Every somatic cell can theoretically become a hiPSC and give rise to targeted cells of the human body. However, there have been debates on the controversy about the differentiation propensity according to the origin of primary cells. We reprogrammed hiPSCs from four different types of primary cells such as dermal fibroblasts (DF, n = 3), peripheral blood mononuclear cells (PBMC, n = 3), cord blood mononuclear cells (CBMC, n = 3), and osteoarthritis fibroblast-like synoviocytes (OAFLS, n = 3)...
2018: Stem Cells International
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