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chondrogenesis

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https://www.readbyqxmd.com/read/28338415/hyaluronic-acid-hydrogel-functionalized-with-self-assembled-micelles-of-amphiphilic-pegylated-kartogenin-for-the-treatment-of-osteoarthritis
#1
Mi-Lan Kang, Se-Young Jeong, Gun-Il Im
Synthetic hyaluronic acid (HA) containing a covalently integrated drug is capable of releasing therapeutic molecules and is an attractive candidate for the intra-articular treatment of osteoarthritis (OA). Herein, self-assembled PEGylated kartogenin (PEG/KGN) micelles consisting of hydrophilic polyethylene glycol (PEG) and hydrophobic KGN, which has been shown to induce chondrogenesis in human mesenchymal stem cells, were prepared by covalent crosslinking. HA hydrogels containing PEG/KGN micelles (HA/PEG/KGN) were prepared by covalently bonding PEG chains to HA...
March 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28335721/transcriptomic-insights-into-the-genetic-basis-of-mammalian-limb-diversity
#2
Jennifer A Maier, Marcelo Rivas-Astroza, Jenny Deng, Anna Dowling, Paige Oboikovitz, Xiaoyi Cao, Richard R Behringer, Chris J Cretekos, John J Rasweiler, Sheng Zhong, Karen E Sears
BACKGROUND: From bat wings to whale flippers, limb diversification has been crucial to the evolutionary success of mammals. We performed the first transcriptome-wide study of limb development in multiple species to explore the hypothesis that mammalian limb diversification has proceeded through the differential expression of conserved shared genes, rather than by major changes to limb patterning. Specifically, we investigated the manner in which the expression of shared genes has evolved within and among mammalian species...
March 23, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28334780/eif4a3-deficient-human-ipscs-and-mouse-models-demonstrate-neural-crest-defects-that-underlie-richieri-costa-pereira-syndrome
#3
Emily E Miller, Gerson S Kobayashi, Camila M Musso, Miranda Allen, Felipe A A Ishiy, Luiz C de Caires Junior, Ernesto S G Guimarães, Karina Griesi-Oliveira, Roseli M Zechi-Ceide, Antonio Richieri-Costa, Debora R Bertola, Maria Rita Passos-Bueno, Debra L Silver
Biallelic loss-of-function mutations in the RNA binding protein EIF4A3 cause Richieri-Costa-Pereira syndrome (RCPS), an autosomal recessive condition mainly characterized by craniofacial and limb malformations. However, the pathogenic cellular mechanisms responsible for this syndrome are entirely unknown. Here we used two complementary approaches, patient-derived induced pluripotent stem cells (iPSCs) and conditional Eif4a3 mouse models, to demonstrate that defective Neural Crest Cell (NCC) development explains RCPS craniofacial abnormalities...
March 2, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28334714/new-genetic-diagnoses-of-short-stature-provide-insights-into-local-regulation-of-childhood-growth%C3%A2
#4
Anenisia C Andrade, Youn Hee Jee, Ola Nilsson
Idiopathic short stature is a common condition with a heterogeneous etiology. Advances in genetic methods, including genome sequencing techniques and bioinformatics approaches, have emerged as important tools to identify the genetic defects in families with monogenic short stature. These findings have contributed to the understanding of growth regulation and indicate that growth plate chondrogenesis, and therefore linear growth, is governed by a large number of genes important for different signaling pathways and cellular functions, including genetic defects in hormonal regulation, paracrine signaling, cartilage matrix, and fundamental cellular processes...
March 23, 2017: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/28332587/bioreactor-mechanically-guided-3d-mesenchymal-stem-cell-chondrogenesis-using-a-biocompatible-novel-thermo-reversible-methylcellulose-based-hydrogel
#5
A Cochis, S Grad, M J Stoddart, S Farè, L Altomare, B Azzimonti, M Alini, L Rimondini
Autologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes' poor expansion capacity in vitro. Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells. Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332167/controlled-release-of-vanadium-from-a-composite-scaffold-stimulates-mesenchymal-stem-cell-osteochondrogenesis
#6
S D Schussler, K Uske, P Marwah, F W Kemp, J D Bogden, S S Lin, Treena Livingston Arinzeh
Large bone defects often require the use of autograft, allograft, or synthetic bone graft augmentation; however, these treatments can result in delayed osseous integration. A tissue engineering strategy would be the use of a scaffold that could promote the normal fracture healing process of endochondral ossification, where an intermediate cartilage phase is later transformed to bone. This study investigated vanadyl acetylacetonate (VAC), an insulin mimetic, combined with a fibrous composite scaffold, consisting of polycaprolactone with nanoparticles of hydroxyapatite and beta-tricalcium phosphate, as a potential bone tissue engineering scaffold...
March 22, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28326409/chondrogenesis-in-the-synovial-tissue-is-associated-with-the-onset-of-pseudogout-after-total-knee-arthroplasty
#7
Ryosuke Sato, Shunji Nakano, Tomoya Takasago, Masaru Nakamura, Masahiro Kashima, Takashi Chikawa, Daisuke Hamada, Tomohiro Goto, Koichi Sairyo
Postoperative pseudogout after total knee arthroplasty (TKA) is very rare, and its physical findings are very similar to infectious symptoms. In pseudogout, the mechanism underlying the deposition of calcium pyrophosphate dehydrate crystals remains unclear. Here, we report the histologic findings in a pseudogout attack in the late postoperative period after TKA. She had acute onset of arthritis of the knee 2 years after TKA. Histologic examination showed significant neutrophil infiltration. Interestingly, chondrogenesis was noted in the synovial tissue, and calcium pyrophosphate dehydrate crystals were synthesized mainly at the site of chondrogenesis, suggesting a potential mechanism underlying the occurrence of pseudogout after TKA...
September 2016: Arthroplasty Today
https://www.readbyqxmd.com/read/28324152/good-clinical-results-with-autologous-matrix-induced-chondrogenesis-amic-technique-in-large-knee-chondral-defects
#8
Alfredo Schiavone Panni, Chiara Del Regno, Giuseppe Mazzitelli, Rocco D'Apolito, Katia Corona, Michele Vasso
PURPOSE: Autologous matrix-induced chondrogenesis (AMIC) is a treatment for focal full-thickness cartilage defects combining microfracturing with an exogenous I/III collagen matrix (Chondro-Gide). The aim of the present study was to determine the 7 years outcomes of patients treated with the AMIC technique for knee chondral defects larger than 2 cm(2). The hypothesis was that the positive short-term outcomes achieved in the previous series would not deteriorate at a 7-year follow-up...
March 21, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28317389/prp-for-degenerative-cartilage-disease
#9
Lior Laver, Niv Marom, Lad Dnyanesh, Omer Mei-Dan, João Espregueira-Mendes, Alberto Gobbi
OBJECTIVE: To explore the utilization of platelet-rich plasma (PRP) for degenerative cartilage processes and evaluate whether there is sufficient evidence to better define its potential effects. DESIGN: Systematic literature reviews were conducted in PubMed/MEDLINE and Cochrane electronic databases till May 2015, using the keywords "platelet-rich plasma OR PRP OR autologous conditioned plasma OR ACP AND cartilage OR chondrocyte OR chondrogenesis OR osteoarthritis (OA) OR arthritis...
September 1, 2016: Cartilage
https://www.readbyqxmd.com/read/28314754/kdm6b-regulates-cartilage-development-and-homeostasis-through-anabolic-metabolism
#10
Jun Dai, Dongsheng Yu, Yafei Wang, Yishan Chen, Heng Sun, Xiaolei Zhang, Shouan Zhu, Zongyou Pan, Boon Chin Heng, Shufang Zhang, Hongwei Ouyang
OBJECTIVES: Epigenetic mechanisms have been reported to play key roles in chondrogenesis and osteoarthritis (OA) development. Here, we sought to identify specific histone demethylases that are involved and delineate the underlying mechanisms. METHODS: We screened the expression of 17 distinct histone demethylases by quantitative real time PCR (qRT-PCR) during chondrogenic differentiation of C3H10T1/2 cells. The role of Kdm6b in cartilage development was then analysed with transgenic Col2a1-CreER(T2);Kdm6b(f/f) ...
March 17, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28304100/cartilage-specific-autophagy-deficiency-promotes-er-stress-and-impairs-chondrogenesis-in-perk-atf4-chop-dependent-manner
#11
Xiaomin Kang, Wei Yang, Dongxu Feng, Xinxin Jin, Zhengmin Ma, Zhuang Qian, Tianping Xie, Huixia Li, Jiali Liu, Ruiqi Wang, Fang Li, Danhui Li, Hongzhi Sun, Shufang Wu
Autophagy is activated during nutritionally depleted or hypoxic conditions to facilitate cell survival. As growth plate is an avascular and hypoxic tissue, autophagy may have a crucial role during chondrogenesis, however the functional role and underlying mechanism of autophagy in regulation of growth plate remains elusive. In this study, we generated (TamCart) Atg7(-/-) (Atg7cKO) mice to explore the role of autophagy during endochondral ossification. Atg7cKO mice exhibited growth retardation associated with reduced chondrocyte proliferation, differentiation and increased chondrocyte apoptosis...
March 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28302495/neural-egfl-like-1-is-a-downstream-regulator-of-runt-related-transcription-factor-2-in-chondrogenic-differentiation-and-maturation
#12
Chenshuang Li, Jie Jiang, Zhong Zheng, Kevin S Lee, Yanheng Zhou, Eric Chen, Cymbeline T Culiat, Yiqiang Qiao, Xuepeng Chen, Kang Ting, Xinli Zhang, Chia Soo
Recent studies indicate that neural EGFL-like 1 (Nell-1), a secretive extracellular matrix molecule, is involved in chondrogenic differentiation. Herein, we demonstrated that Nell-1 serves as a key downstream target of runt-related transcription factor 2 (Runx2), a central regulator of chondrogenesis. Unlike in osteoblast lineage cells where Nell-1 and Runx2 demonstrate mutual regulation, further studies in chondrocytes revealed that Runx2 tightly regulates the expression of Nell-1; however, Nell-1 does not alter the expression of Runx2...
March 13, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28300491/dynamic-regulation-of-twist1-expression-during-chondrogenic-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells
#13
Mairéad A Cleary, Roberto Narcisi, Anna Albiero, Florien Jenner, Laurie M G de Kroon, Wendy J L M Koevoet, Pieter A J Brama, Gerjo J V M van Osch
Human bone marrow-derived mesenchymal stem cells (BMSCs) are clinically promising to repair damaged articular cartilage. This study investigated TWIST1, an important transcriptional regulator in mesenchymal lineages, in BMSC chondrogenesis. We hypothesized that downregulation of TWIST1 expression is required for in vitro chondrogenic differentiation. Indeed, significant downregulation of TWIST1 was observed in murine skeletal progenitor cells during limb development (N = 3 embryos), and during chondrogenic differentiation of culture-expanded human articular chondrocytes (N = 3 donors) and isolated adult human BMSCs (N = 7 donors), consistent with an inhibitory effect of TWIST1 expression on chondrogenic differentiation...
March 16, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28295277/lipopolysaccharide-can-modify-differentiation-and-immunomodulatory-potential-of-periodontal-ligament-stem-cells-via-erk1-2-signaling
#14
Tamara Kukolj, Drenka Trivanović, Ivana Okić Djordjević, Slavko Mojsilović, Jelena Krstić, Hristina Obradović, Srdja Janković, Juan Francisco Santibanez, Aleksandra Jauković, Diana Bugarski
Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (E.coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPARγ mRNA expression...
March 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28287507/chondrogenic-differentiation-induction-of-adipose-derived-stem-cells-by-centrifugal-gravity
#15
Yeonsue Jang, Hyerin Jung, Ji Hyeon Ju
Impaired cartilage cannot heal naturally. Currently, the most advanced therapy for defects in cartilage is the transplantation of chondrocytes differentiated from stem cells using cytokines. Unfortunately, cytokine-induced chondrogenic differentiation is costly, time-consuming, and associated with a high risk of contamination during in vitro differentiation. However, biomechanical stimuli also serve as crucial regulatory factors for chondrogenesis. For example, mechanical stress can induce chondrogenic differentiation of stem cells, suggesting a potential therapeutic approach for the repair of impaired cartilage...
February 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28285569/gelatin-scaffolds-containing-partially-sulfated-cellulose-promote-mesenchymal-stem-cell-chondrogenesis
#16
Gloria Portocarrero Huang, Roseline Menezes, Richard Vincent, Willis Hammond, Louis Rizio, George Collins, Treena Livingston Arinzeh
Articular cartilage has a limited capacity to heal after damage from injury or degenerative disease. Tissue engineering constructs that more closely mimic the native cartilage microenvironment can be utilized to promote repair. Glycosaminoglycans (GAGs), a major component of the cartilage extracellular matrix, have the ability to sequester growth factors due to their level and spatial distribution of sulfate groups. This study evaluated the use of a GAG mimetic, cellulose sulfate, as a scaffolding material for cartilage tissue engineering...
March 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28282672/all-arthroscopic-autologous-matrix-induced-chondrogenesis-aided-repair-of-a-patellar-cartilage-defect-using-dry-arthroscopy-and-a-retraction-system
#17
Boguslaw Sadlik, Mariusz Puszkarz, Lidia Kosmalska, Martin Wiewiorski
The technique of all-arthroscopic autologous matrix-induced chondrogenesis (AMIC)-aided repair of patellar cartilage lesions using a retraction system and dry arthroscopy has been recently described. We report the first clinical and radiological data at a short-term follow-up. Twelve patients underwent AMIC-aided cartilage repair for a patellar lesion. All steps of the procedure were performed arthroscopically, which include the use of an intra-articularly placed retraction plate for distraction of the patellofemoral joint and evacuation of saline solution for collagen matrix insertion and fixation...
March 10, 2017: Journal of Knee Surgery
https://www.readbyqxmd.com/read/28280669/in-vivo-chondrogenesis-in-3d-bioprinted-human-cell-laden-hydrogel-constructs
#18
Thomas Möller, Matteo Amoroso, Daniel Hägg, Camilla Brantsing, Nicole Rotter, Peter Apelgren, Anders Lindahl, Lars Kölby, Paul Gatenholm
BACKGROUND: The three-dimensional (3D) bioprinting technology allows creation of 3D constructs in a layer-by-layer fashion utilizing biologically relevant materials such as biopolymers and cells. The aim of this study is to investigate the use of 3D bioprinting in a clinically relevant setting to evaluate the potential of this technique for in vivo chondrogenesis. METHODS: Thirty-six nude mice (Balb-C, female) received a 5- × 5- × 1-mm piece of bioprinted cell-laden nanofibrillated cellulose/alginate construct in a subcutaneous pocket...
February 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28280656/noninvasive-measurement-of-ear-cartilage-elasticity-on-the-cellular-level-a-new-method-to-provide-biomechanical-information-for-tissue-engineering
#19
Ernst Jan Bos, Koen van der Laan, Marco N Helder, Margriet G Mullender, Davide Iannuzzi, Paul P van Zuijlen
BACKGROUND: An important feature of auricular cartilage is its stiffness. To tissue engineer new cartilage, we need objective tools to provide us with the essential biomechanical information to mimic optimal conditions for chondrogenesis and extracellular matrix (ECM) development. In this study, we used an optomechanical sensor to investigate the elasticity of auricular cartilage ECM and tested whether sensitivity and measurement reproducibility of the sensor would be sufficient to accurately detect (subtle) differences in matrix compositions in healthy, diseased, or regenerated cartilage...
February 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28265109/comparative-evaluation-of-leukocyte-and-platelet-rich-plasma-and-pure-platelet-rich-plasma-for-cartilage-regeneration
#20
Zhengliang Xu, Wenjing Yin, Yuelei Zhang, Xin Qi, Yixuan Chen, Xuetao Xie, Changqing Zhang
Platelet-rich plasma (PRP) has gained growing popularity in the treatment of articular cartilage lesions in the last decade. However, the potential harmful effects of leukocytes in PRP on cartilage regeneration have seldom been studied in vitro, and not at all in vivo yet. The objective of the present study is to compare the effects of leukocyte- and platelet-rich plasma (L-PRP) and pure platelet-rich plasma (P-PRP) on cartilage repair and NF-κB pathway, in order to explore the mechanism underlying the function of leukocytes in PRP in cartilage regeneration...
March 7, 2017: Scientific Reports
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