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https://www.readbyqxmd.com/read/28088667/cell-laden-hydrogels-for-osteochondral-and-cartilage-tissue-engineering
#1
REVIEW
Jingzhou Yang, Yu Shrike Zhang, Kan Yue, Ali Khademhosseini
: Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue engineered artificial matrices that can replace the damaged regions and promote tissue regeneration. Hydrogels are emerging as a promising class of biomaterials for both soft and hard tissue regeneration. Many critical properties of hydrogels, such as mechanical stiffness, elasticity, water content, bioactivity, and degradation, can be rationally designed and conveniently tuned by proper selection of the material and chemistry...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28087486/enhanced-articular-cartilage-by-human-mesenchymal-stem-cells-in-enzymatically-mediated-transiently-rgds-functionalized-collagen-mimetic-hydrogels
#2
Paresh A Parmar, Jean-Philippe St-Pierre, Lesley W Chow, Christopher D Spicer, Violet Stoichevska, Yong Y Peng, Jerome A Werkmeister, John A M Ramshaw, Molly M Stevens
: Recapitulation of the articular cartilage microenvironment for regenerative medicine applications faces significant challenges due to the complex and dynamic biochemical and biomechanical nature of native tissue. Towards the goal of biomaterial designs that enable the temporal presentation of bioactive sequences, recombinant bacterial collagens such as Streptococcal collagen-like 2 (Scl2) proteins can be employed to incorporate multiple specific bioactive and biodegradable peptide motifs into a single construct...
January 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28076994/adapted-dexamethasone-delivery-polyethylene-oxide-and-poly-%C3%A9-caprolactone-construct-promote-mesenchymal-stem-cells-chondrogenesis
#3
Armaghan Ghiaee, Faeze Pournaqi, Saeid Vakilian, Abdolah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi
Issues associated with tissue transplantation and shortage of donors has always been a concern, whereas tissue engineering has provided the hopeful opportunities. The aim of this study was to investigate the chondrogenic differentiation potential of mesenchymal stem cells (MSCs) in the presence of embedded Dexamethasone into electrospun Poly(ethyleneoxide) nanofibers composited with Poly(ɛ-caprolactone) nanofibers. Electrospun-fabricated scaffolds were characterized by SEM, tensile, contact angle, release profile, MTT assay, and chonderogenic differentiation of stem cells...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28076977/combining-microfractures-autologous-bone-graft-and-autologous-matrix-induced-chondrogenesis-for-the-treatment-of-juvenile-osteochondral-talar-lesions
#4
Riccardo D'Ambrosi, Camilla Maccario, Chiara Ursino, Nicola Serra, Federico Giuseppe Usuelli
BACKGROUND: The purpose of this study was to evaluate the clinical and radiologic outcomes of patients younger than 20 years, treated with the arthroscopic-talus autologous matrix-induced chondrogenesis (AT-AMIC) technique and autologous bone graft for osteochondral lesion of the talus (OLT). METHODS: Eleven patients under 20 years (range 13.3-20.0) underwent the AT-AMIC procedure and autologous bone graft for OLTs. Patients were evaluated preoperatively (T0) and at 6 (T1), 12 (T2), and 24 (T3) months postoperatively, using the American Orthopaedic Foot & Ankle Society Ankle and Hindfoot (AOFAS) score, the visual analog scale and the SF-12 respectively in its Mental and Physical Component Scores...
January 1, 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/28069589/analysis-of-the-fgfr2c342y-mouse-model-shows-condensation-defects-due-to-misregulation-of-sox9-expression-in-prechondrocytic-mesenchyme
#5
Emma Peskett, Samin Kumar, William Baird, Janhvi Jaiswal, Ming Li, Priyanca Patel, Jonathan A Britto, Erwin Pauws
Syndromic craniosynostosis caused by mutations in FGFR2 is characterised by developmental pathology in both endochondral and membranous skeletogenesis. Detailed phenotypic characterisation of features in the membranous calvarium, the endochondral cranial base and other structures in the axial and appendicular skeleton has not been performed at embryonic stages. We investigated bone development in the Crouzon mouse model (Fgfr2(C342Y)) at pre- and post-ossification stages to improve understanding of the underlying pathogenesis...
January 9, 2017: Biology Open
https://www.readbyqxmd.com/read/28066742/autologous-membrane-induced-chondrogenesis-amic-for-the-treatment-of-acetabular-chondral-defect
#6
Andrea Fontana
BACKGROUND: Acetabular chondral defect are very frequently associated to FAI. Treatment options are still questionable. METHODS: Between 2008 and 2014, 201 patients over 583 have been arthroscopically treated with the AMIC procedure for grade III and/or IV acetabular chondral lesions. Patients age was between 18 and 50 years; acetabular chondral lesion size was between 2 and 4 cm(2); radiological Tönnis degree of osteoarthritis was ≤ 2. RESULTS: The mean follow up of the entire group of 201 patients was 5 years (from 8 to 2)...
July 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/28060847/enhanced-chondrogenic-differentiation-of-human-umbilical-cord-wharton-s-jelly-derived-mesenchymal-stem-cells-by-gsk-3-inhibitors
#7
Prapot Tanthaisong, Sumeth Imsoonthornruksa, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Mariena Ketudat-Cairns, Rangsun Parnpai
Articular cartilage is an avascular, alymphatic, and aneural system with very low regeneration potential because of its limited capacity for self-repair. Mesenchymal stem cells (MSCs) are the preferred choice for cell-based therapies. Glycogen synthase kinase 3 (GSK-3) inhibitors are compounds that can induce the Wnt signaling pathway, which is involved in chondrogenesis and cartilage development. Here, we investigated the influence of lithium chloride (LiCl) and SB216763 synergistically with TGF-β3 on chondrogenic differentiation in human mesenchymal stem cells derived from Wharton's jelly tissue (hWJ-MSCs)...
2017: PloS One
https://www.readbyqxmd.com/read/28060328/culture-of-murine-embryonic-metatarsals-a-physiological-model-of-endochondral-ossification
#8
Dean A Houston, Katherine A Staines, Vicky E MacRae, Colin Farquharson
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth. As such, it is imperative that we further our understanding of the underpinning molecular mechanisms involved in such disorders so as to provide advances towards human and animal patient benefit. The mouse metatarsal organ explant culture is a highly physiological ex vivo model for studying endochondral ossification and bone growth as the growth rate of the bones in culture mimic that observed in vivo...
December 3, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28059449/irx3-and-bmp2-regulate-mouse-mesenchymal-cell-chondrogenic-differentiation-in-both-a-sox9-dependent-and-independent-manner
#9
Yoshihiro Tamamura, Kenichi Katsube, Hisashi Mera, Maki Itokazu, Shigeyuki Wakitani
Sox9, a master regulator of cartilage development, controls the cell fate decision to differentiate from mesenchymal to chondrogenic cells. In addition, Sox9 regulates the proliferation and differentiation of chondrocytes, as well as the production of cartilage-specific proteoglycans. The existence of Sox9-independent mechanisms in cartilage development remains to be determined. Here, we attempted to identify genes involved in such putative mechanisms via microarray analysis using a mouse chondrogenic cell line, N1511...
January 6, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28054960/evaluation-of-magnetic-nanoparticle-labeled-chondrocytes-cultivated-on-a-type-ii-collagen-chitosan-poly-lactic-co-glycolic-acid-biphasic-scaffold
#10
Juin-Yih Su, Shi-Hui Chen, Yu-Pin Chen, Wei-Chuan Chen
Chondral or osteochondral defects are still controversial problems in orthopedics. Here, chondrocytes labeled with magnetic nanoparticles were cultivated on a biphasic, type II collagen-chitosan/poly(lactic-co-glycolic acid) scaffold in an attempt to develop cultures with trackable cells exhibiting growth, differentiation, and regeneration. Rabbit chondrocytes were labeled with magnetic nanoparticles and characterized by scanning electron microscopy (SEM), transmission electron (TEM) microscopy, and gene and protein expression analyses...
January 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28043752/fibrous-synovium-releases-higher-numbers-of-mesenchymal-stem-cells-than-adipose-synovium-in-a-suspended-synovium-culture-model
#11
Kenta Katagiri, Yu Matsukura, Takeshi Muneta, Nobutake Ozeki, Mitsuru Mizuno, Hisako Katano, Ichiro Sekiya
PURPOSE: To develop an in vitro model, the "suspended synovium culture model," to demonstrate the mobilization of mesenchymal stem cells (MSCs) from the synovium into a noncontacted culture dish through culture medium. In addition, to examine which synovium, fibrous synovium or adipose synovium, released more MSCs in the knee with osteoarthritis. METHODS: Human synovial tissue was harvested during total knee arthroplasty from knee joints of 34 patients with osteoarthritis (28 patients: only fibrous synovium, 6 patients: fibrous and adipose synovium)...
December 30, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28039148/gpx1-knockdown-suppresses-chondrogenic-differentiation-of-atdc5-cells-through-induction-of-reductive-stress
#12
Jidong Yan, Yuanxu Guo, Yao Fei, Rui Zhang, Yan Han, Shemin Lu
Glutathione peroxidase 1 (GPx1) is a selenium (Se)-containing protein and is induced in cartilage formation. GPx1 eliminates reactive oxygen species (ROS), which are required for chondrogenic induction. The physiological properties of GPx1 in cartilage and the redox mechanisms involved are not known. The effects of GPx1 on chondrogenic differentiation of ATDC5 cells were examined through short hairpin RNA-mediated gene silencing. The results demonstrated that GPx1 knockdown impaired gene expression of sex determining region Y-box 9, collagen II (Col II), and aggrecan...
December 29, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28038353/decellularized-adipose-tissue-microcarriers-as-a-dynamic-culture-platform-for-human-adipose-derived-stem-stromal-cell-expansion
#13
Claire Yu, Anna Kornmuller, Cody Brown, Todd Hoare, Lauren E Flynn
With the goal of designing a clinically-relevant expansion strategy for human adipose-derived stem/stromal cells (ASCs), methods were developed to synthesize porous microcarriers derived purely from human decellularized adipose tissue (DAT). An electrospraying approach was applied to generate spherical DAT microcarriers with an average diameter of 428 ± 41 μm, which were soft, compliant, and stable in long-term culture without chemical crosslinking. Human ASCs demonstrated enhanced proliferation on the DAT microcarriers relative to commercially-sourced Cultispher-S microcarriers within a spinner culture system over 1 month...
March 2017: Biomaterials
https://www.readbyqxmd.com/read/28028895/characterization-of-sox9-overexpressing-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-based-engineered-cartilage-both-in-vitro-and-in-vivo
#14
Xiao-Li Li, Jun Zhang, Hua-Nan Luo, Xiaoyan Zhao, A-Ling Zhang, Zheng-Hui Wang
The source of seed cells is a critical factor for tissue engineering . The goal of this study was to evaluate the chondrogenesis of Sox9-overexpressing human umbilical cord mesenchymal stem cells (hUCMSCs) seeded onto bone matrix gelatin (BMG)/fibrin hybrid scaffolds both in vitro and in vivo. hUCMSCs were stably transfected with Sox9-expressing plasmid and grown on the three-dimensional BMG/fibrin hybrid scaffold for 8 weeks. Scanning electron microscopy and histochemistry were performed. The hUCMSC-loaded scaffolds were implanted into the subcutaneous layer of immunocompetent rats and chondrogenesis and host immune responses were monitored for 8 weeks...
December 28, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28025887/influence-of-crosslinkers-on-the-in-vitro-chondrogenesis-of-mesenchymal-stem-cells-in-hyaluronic-acid-hydrogels
#15
Panita Maturavongsadit, Xiangdong Bi, Kamolrat Metavarayuth, Jittima Amie Luckanagul, Qian Wang
This study aims to investigate the effect of the structures of crosslinkers on the in vitro chondrogenic differentiation of bone mesenchymal stem cells (BMSCs) in hyaluronic acid (HA)-based hydrogels. The hydrogels were prepared by the covalent crosslinking of methacrylated HA with different types of thiol-tailored molecules, including dithiothreitol (DTT), 4-arm polyethylene glycol (PEG), and multi-arm polyamidoamine (PAMAM) dendrimer using thiol-ene "click" chemistry. The microstructure, mechanical properties, diffusivity and degradation rates of the resultant hydrogels were controlled by the structural feature of different crosslinkers...
December 27, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28024600/chondrogenic-differentiation-of-atdc5-cells-under-the-influence-of-mg-and-mg-alloy-degradation
#16
Adela H Martinez Sanchez, Frank Feyerabend, Daniel Laipple, Regine Willumeit-Römer, Annelie Weinberg, Bérengère J C Luthringer
Biodegradable magnesium (Mg)-based materials are a potential alternative to permanent implants for application in children. Nevertheless effects of those materials on growth plate cartilage and chondrogenesis have not been previously evaluated. In vitro differentiation of ATDC5 cells was evaluated under the influence of pure Mg (PMg), Mg with 10wt% of gadolinium (Mg-10Gd) and Mg with 2wt% of silver (Mg-2Ag) degradation products (extracts) and direct cell culture on the materials. Gene expression showed an inhibitory effect on ATDC5 mineralization with the three extracts and a chondrogenic potential of Mg-10Gd...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28011039/the-direction-of-human-mesenchymal-stem-cells-into-the-chondrogenic-lineage-is-influenced-by-the-features-of-hydrogel-carriers
#17
A Hansson, A Wenger, H Barreto Henriksson, S Li, B R Johansson, H Brisby
Low back pain is a major public health issue in the Western world, one main cause is believed to be intervertebral disc (IVD) degeneration. To halt/diminish IVD degeneration, cell therapy using different biomaterials e.g. hydrogels as cell carriers has been suggested. In this study, two different hydrogels were examined (in vitro) as potential cell carriers for human mesenchymal stem cells (hMSCs) intended for IVD transplantation. The aim was to investigate cell-survival and chondrogenic differentiation of hMSCs when cultured in hydrogels Puramatrix(®) or Hydromatrix(®) and potential effects of stimulation with growth hormone (GH)...
December 13, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28005327/construction-of-plga-nanoparticles-coated-with-polycistronic-sox5-sox6-and-sox9-genes-for-chondrogenesis-of-human-mesenchymal-stem-cells
#18
Ji Sun Park, Se Won Yi, Hye Jin Kim, Seong Min Kim, Jae-Hwan Kim, Keun-Hong Park
Transfection of a cocktail of genes into cells has recently attracted attraction in stem cell differentiation. However, it is not easy to control the transfection rate of each gene. To control and regulate gene delivery into human mesenchymal stem cells (hMSCs), we employed multicistronic genes coupled with a nonviral gene carrier system for stem cell differentiation. Three genes, SOX5, SOX6, and SOX9, were successfully fabricated in a single plasmid. This multicistronic plasmid was complexed with the polycationic polymer polyethylenimine, and poly(lactic-co-glycolic) acid (PLGA) nanoparticles were coated with this complex...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28004813/electrical-stimulation-drives-chondrogenesis-of-mesenchymal-stem-cells-in-the-absence-of-exogenous-growth-factors
#19
Hyuck Joon Kwon, Gyu Seok Lee, Honggu Chun
Electrical stimulation (ES) is known to guide the development and regeneration of many tissues. However, although preclinical and clinical studies have demonstrated superior effects of ES on cartilage repair, the effects of ES on chondrogenesis remain elusive. Since mesenchyme stem cells (MSCs) have high therapeutic potential for cartilage regeneration, we investigated the actions of ES during chondrogenesis of MSCs. Herein, we demonstrate for the first time that ES enhances expression levels of chondrogenic markers, such as type II collagen, aggrecan, and Sox9, and decreases type I collagen levels, thereby inducing differentiation of MSCs into hyaline chondrogenic cells without the addition of exogenous growth factors...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27999805/efficiency-of-human-epiphyseal-chondrocytes-with-differential-replication-numbers-for-cellular-therapy-products
#20
Michiyo Nasu, Shinichiro Takayama, Akihiro Umezawa
The cell-based therapy for cartilage or bone requires a large number of cells; serial passages of chondrocytes are, therefore, needed. However, fates of expanded chondrocytes from extra fingers remain unclarified. The chondrocytes from human epiphyses morphologically changed from small polygonal cells to bipolar elongated spindle cells and to large polygonal cells with degeneration at early passages. Gene of type II collagen was expressed in the cells only at a primary culture (Passage 0) and Passage 1 (P1) cells...
2016: BioMed Research International
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