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https://www.readbyqxmd.com/read/29454076/the-biological-basis-of-treating-jaw-discrepancies-an-interplay-of-mechanical-forces-and-skeletal-configuration
#1
REVIEW
Konstantinos Karamesinis, Efthimia K Basdra
Jaw discrepancies and malrelations affect a large proportion of the general population and their treatment is of utmost significance for individuals' health and quality of life. The aim of their therapy is the modification of aberrant jaw development mainly by targeting the growth potential of the mandibular condyle through its cartilage, and the architectural shape of alveolar bone through a suture type of structure, the periodontal ligament. This targeted treatment is achieved via external mechanical force application by using a wide variety of intraoral and extraoral appliances...
February 14, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29448837/restoring-tracheal-defects-in-a-rabbit-model-with-tissue-engineered-patches-based-on-tgf-%C3%AE-3-encapsulating-electrospun-poly-l-lactic-acid-co-%C3%AE%C2%B5-caprolactone-collagen-scaffolds
#2
Hui Jing, Botao Gao, Manchen Gao, Haiyue Yin, Xiumei Mo, Xiaoyang Zhang, Kai Luo, Bei Feng, Wei Fu, Jing Wang, Wei Zhang, Meng Yin, Zhongqun Zhu, Xiaomin He, Jinghao Zheng
Long segment tracheal stenosis often has a poor prognosis due to the limited availability of materials for tracheal reconstruction. Tissue engineered tracheal patches based on electrospun scaffolds and stem cells present ideal solutions to this medical challenge. However, the established engineering process is inefficient and time-consuming. In our research, to optimize the engineering process, core-shell nanofilms encapsulating TGF-β3 were fabricated as scaffolds for tracheal patches. The morphological and mechanical characteristics, degradation and biocompatibility of poly(l-lactic acid-co-ε-caprolactone)/collagen (PLCL/collagen) scaffolds with different compositions (PLCL:collagen 75:25, 50:50 and 25:75, respectively) were comparatively evaluated to determine the preferable compositional ratio...
February 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29448134/enhancing-proliferation-and-optimizing-the-culture-condition-for-human-bone-marrow-stromal-cells-using-hypoxia-and-fibroblast-growth-factor-2
#3
Jung-Seok Lee, Seul Ki Kim, Byung-Joo Jung, Seong-Bok Choi, Eun-Young Choi, Chang-Sung Kim
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation...
January 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29447948/roles-of-ihh-signaling-in-chondroprogenitor-function-in-postnatal-condylar-cartilage
#4
Naito Kurio, Cheri Saunders, Till E Bechtold, Imad Salhab, Hyun-Duck Nah, Sayantani Sinha, Paul C Billings, Maurizio Pacifici, Eiki Koyama
Condylar articular cartilage in mouse temporomandibular joint develops from progenitor cells near the articulating surface that proliferate, undergo chondrogenesis and mature into hypertrophic chondrocytes. However, it remains unclear how these processes are regulated, particularly postnatally. Here we focused on the apical polymorphic layer rich in progenitors and asked whether the phenotype and fate of the cells require signaling by Indian hedgehog (Ihh) previously studied in developing long bones. In condyles in newborn mice, the apical polymorphic/progenitor cell layer was ~10 cell layer-thick and expressed the articular matrix marker Tenascin-C (Tn-C), and the underlying thick cell layer expressed Tn-C as well as the chondrogenic master regulator Sox9...
February 12, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29426836/combinatorial-hydrogels-with-biochemical-gradients-for-screening-3d-cellular-microenvironments
#5
Sebastián L Vega, Mi Y Kwon, Kwang Hoon Song, Chao Wang, Robert L Mauck, Lin Han, Jason A Burdick
3D microenvironmental parameters control cell behavior, but can be challenging to investigate over a wide range of conditions. Here, a combinatorial hydrogel platform is developed that uses light-mediated thiol-norbornene chemistry to encapsulate cells within hydrogels with biochemical gradients made by spatially varied light exposure. Specifically, mesenchymal stem cells are photoencapsulated in norbornene-modified hyaluronic acid hydrogels functionalized with gradients (0-5 mM) of peptides that mimic cell-cell or cell-matrix interactions, either as single or orthogonal gradients...
February 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29421560/dual-non-viral-gene-delivery-from-microparticles-within-3d-high-density-stem-cell-constructs-for-enhanced-bone-tissue-engineering
#6
Alexandra McMillan, Minh Khanh Nguyen, Tomas Gonzalez-Fernandez, Peilin Ge, Xiaohua Yu, William L Murphy, Daniel J Kelly, Eben Alsberg
High-density mesenchymal stem cell (MSC) aggregates can be guided to form bone-like tissue via endochondral ossification in vitro when culture media is supplemented with proteins, such as growth factors (GFs), to first guide the formation of a cartilage template, followed by culture with hypertrophic factors. Recent reports have recapitulated these results through the controlled spatiotemporal delivery of chondrogenic transforming growth factor-β1 (TGF-β1) and chondrogenic and osteogenic bone morphogenetic protein-2 (BMP-2) from microparticles embedded within human MSC aggregates to avoid diffusion limitations and the lengthy, costly in vitro culture necessary with repeat exogenous supplementation...
January 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29409254/the-role-of-bone-marrow-edema-on-osteochondral-lesions-of-the-talus
#7
Riccardo D'Ambrosi, Camilla Maccario, Chiara Ursino, Nicola Serra, Federico Giuseppe Usuelli
BACKGROUND: To assess the functional and radiological outcomes after arthroscopic talus autologous matrix-induced chondrogenesis (AT-AMIC®) in 2 groups: patients with and without bone marrow edema (BME). METHODS: Thirty-seven patients of which 24 without edema (GNE) and 13 with edema (GE) were evaluated. All patients were treated with AT-AMIC® repair for symptomatic osteochondral talar lesion. Clinical and radiological parameters were evaluated with VAS score for pain, AOFAS and SF-12 at T0 (preoperatively), T1 (6 months), T2 (12 months), T3 (24 months) and MRI and CT-scan at T0, T1, T2 and T3...
March 6, 2017: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29400583/glycol-chitosan-oxidized-hyaluronic-acid-hydrogels-functionalized-with-cartilage-extracellular-matrix-particles-and-incorporating-bmscs-for-cartilage-repair
#8
Chun Liu, Deshuai Liu, Yingying Wang, Yun Li, Tao Li, Zhiyou Zhou, Zhijian Yang, Jianhua Wang, Qiqing Zhang
In this article, we fabricated a bioactive hydrogel composed of glycol chitosan (G-CS) and oxidized hyaluronic acid (OHA) via Schiff base reaction. Cartilage extracellular matrix (ECM) particles with different concentrations were used to functionalize G-CS/OHA (S1) hydrogel. The results demonstrated that S3 (G-CS/OHA/ECM 2% w/v) hydrogel exhibited the most suitable compression strength and provided the optimal environment for proliferation of bone marrow mesenchymal stem cells (BMSCs). To assess the chondroinductivity of ECM in vitro, we compared the chondrogenesis of BMSCs in S1 (G-CS/OHA) and S3 (G-CS/OHA/ECM 2% w/v) hydrogels...
February 5, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29399319/engineering-large-cartilage-tissues-using-dynamic-bioreactor-culture-at-defined-oxygen-conditions
#9
Andrew C Daly, Binulal N Sathy, Daniel J Kelly
Mesenchymal stem cells maintained in appropriate culture conditions are capable of producing robust cartilage tissue. However, gradients in nutrient availability that arise during three-dimensional culture can result in the development of spatially inhomogeneous cartilage tissues with core regions devoid of matrix. Previous attempts at developing dynamic culture systems to overcome these limitations have reported suppression of mesenchymal stem cell chondrogenesis compared to static conditions. We hypothesize that by modulating oxygen availability during bioreactor culture, it is possible to engineer cartilage tissues of scale...
January 2018: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29398623/platelet-lysate-enhances-synovial-fluid-multipotential-stromal-cells-functions-implications-for-therapeutic-use
#10
Ala Altaie, Thomas G Baboolal, Owen Wall, Elena Jones, Dennis McGonagle
BACKGROUND AIMS: Although intra-articular injection of platelet products is increasingly used for joint regenerative approaches, there are few data on their biological effects on joint-resident multipotential stromal cells (MSCs), which are directly exposed to the effects of these therapeutic strategies. Therefore, this study investigated the effect of platelet lysate (PL) on synovial fluid-derived MSCs (SF-MSCs), which in vivo have direct access to sites of cartilage injury. METHODS: SF-MSCs were obtained during knee arthroscopic procedures (N = 7)...
February 2, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29397789/rock-inhibition-promotes-development-of-chondrogenic-tissue-by-improved-mass-transport
#11
Kuo-Chen Wang, Thomas T Egelhoff, Arnold I Caplan, Jean F Welter, Harihara Baskaran
Human mesenchymal stem cell (hMSC)-based chondrogenesis is a key process used to develop tissue engineered cartilage constructs from stem cells, but the resulting constructs have inferior biochemical and biomechanical properties compared to native articular cartilage. Transforming growth factor β containing medium is commonly applied to cell layers of hMSCs, which aggregate upon centrifugation to form 3-D constructs. The aggregation process leads to a high cell density condition, which can cause nutrient limitations during long-term culture, and subsequently, inferior quality of tissue engineered constructs...
February 3, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29394158/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-in-vitro-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#12
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick W Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS + dCM was 63.4%. The sustained release of proteins from MS + dCM was observed over 4 weeks in vitro...
February 2, 2018: Biofabrication
https://www.readbyqxmd.com/read/29393439/role-of-ift88-in-icariin%C3%A2-regulated-maintenance-of-the-chondrocyte-phenotype
#13
Wei Xiang, Jiaming Zhang, Rui Wang, Limei Wang, Shengjie Wang, Yingxing Wu, Yonghui Dong, Fengjing Guo, Tao Xu
Maintenance of the chondrocyte phenotype is crucial for cartilage repair during tissue engineering. Intraflagellar transport protein 88 (IFT88) is an essential component of primary cilia, shuttling signals along the axoneme. The hypothesis of the present study was that IFT88 could exert an important role in icariin‑regulated maintenance of the chondrocyte phenotype. To this end, the effects of icariin on proliferation and differentiation of the chondrogenic cell line, ATDC5, were explored. Icariin‑treated ATDC5 cells and primary chondrocytes expressed IFT88...
January 25, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29393379/role-of-micrornas-in-progenitor-cell-commitment-and-osteogenic-differentiation-in-health-and-disease-review
#14
Maria Teresa Valenti, Luca Dalle Carbonare, Monica Mottes
MicroRNAs (miRNAs) are considered 'micro- managers of gene expression' and awareness of their fundamental role in the control of biological functions is constantly increasing. Bone formation and homeostasis are complex processes involving the differentiation and interaction of various cell types. Several miRNAs have been shown to be involved in different pathways and stages in the regulation of normal and abnormal bone formation and turnover. This present review focuses on the involvement of miRNAs in terms of their effect on the commitment of bone marrow mesenchymal stem cells towards osteogenesis, adipogenesis and chondrogenesis, respectively...
February 1, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29378326/interplay-between-stiffness-and-degradation-of-architectured-gelatin-hydrogels-leads-to-differential-modulation-of-chondrogenesis-in-vitro-and-in-vivo
#15
Melika Sarem, Neha Arya, Miriam Heizmann, Axel T Neffe, Andrea Barbero, Tim P Gebauer, Ivan Martin, Andreas Lendlein, V Prasad Shastri
The limited capacity of cartilage to heal large lesions through endogenous mechanisms has led to extensive effort to develop materials to facilitate chondrogenesis. Although physical-chemical properties of biomaterials have been shown to impactin vitro chondrogenesis, whether these findings are translatable in vivo is subject of debate. Herein, architectured 3D hydrogel scaffolds (ArcGel) (produced by crosslinking gelatin with ethyl lysine diisocyanate (LDI)) were used as a model system to investigate the interplay between scaffold mechanical properties and degradation on matrix deposition by human articular chondrocytes (HAC) from healthy donorsin vitroandin vivo...
January 26, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29374435/a-human-chondrocyte-derived-in-vitro-model-of-alcohol-induced-and-steroid-induced-femoral-head-necrosis
#16
Xiong Qin, Pan Jin, Tongmeng Jiang, Muyan Li, Jiachang Tan, Huayu Wu, Li Zheng, Jinmin Zhao
BACKGROUND Worldwide, femoral head necrosis (FHN), which is also known as avascular necrosis of the femoral head or osteonecrosis of the femoral head, affects millions of people. Excess alcohol intake and steroid use are two common associations with FHN, but their pathogenesis remains unknown. The aim of this study was to develop an in vitro model using human chondrocytes to study alcohol-induced and steroid-induced FHN. MATERIAL AND METHODS In this study, the in vitro model used a monolayer culture of articular chondrocytes derived from patients with non-traumatic FHN (Ficat and Arlet, Stage III)...
January 27, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29373763/comparative-advantages-of-infrapatellar-fat-pad-an-emerging-stem-cell-source-for-regenerative-medicine
#17
Yu Sun, Song Chen, Ming Pei
Growing evidence indicates that infrapatellar fat pad (IPFP)-derived stem cells (IPFSCs) exert robust proliferation capacities and multilineage differentiation potentials. However, few papers summarize the advantages that the IPFP and IPFSCs have in regenerative medicine. In this review we delineate the development and anatomy of the IPFP by comparing it with an adjacent fibrous tissue, synovium, and a more frequently harvested fat depot, subcutaneous adipose tissue. Furthermore, we explore the similarities and differences of stem cells from these three tissues in terms of IPFSCs, synovium-derived stem cells and subcutaneous adipose tissue-derived stem cells in proliferation capacity and tri-lineage differentiation potentials, including chondrogenesis, osteogenesis and adipogenesis...
January 24, 2018: Rheumatology
https://www.readbyqxmd.com/read/29371989/synthesis-and-characterization-of-polyphosphazene-microspheres-incorporating-demineralized-bone-matrix-scaffolds-controlled-release-of-growth-factor-for-chondrogenesis-applications
#18
Bo Ren, Xiaoqing Hu, Jin Cheng, Zhaohui Huang, Pengfei Wei, Weili Shi, Peng Yang, Jiying Zhang, Xiaoning Duan, Qing Cai, Yingfang Ao
As a promising strategy for the successful regeneration of articular cartilage, tissue engineering has received increasing recognition of control release. Two kinds of functional poly (alanine ethyl ester-co-glycine ethyl ester) phosphazene microspheres with different ratios of side-substituent groups were synthesized by emulsion technique. The rate of degradation/hydrolysis of the polymers was carefully tuned to suit the desired application for control release. For controlled delivery of growth factors, the microspheres overcame most of severe side effects linked to demineralized bone matrix (DBM) scaffolds, which had been previously optimized for cartilage regeneration...
December 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/29363151/low-intensity-pulsed-ultrasound-accelerates-traumatic-vertebral-fracture-healing-by-coupling-proliferation-of-type-h-microvessels
#19
Suiyi Wu, Ximing Xu, Jingchuan Sun, Yao Zhang, Jiangang Shi, Tianming Xu
OBJECTIVES: Patients with traumatic vertebral fractures often have major associated postoperative morbidities such as healing failure and kyphosis. Low-intensity pulsed ultrasound (US) has been found to promote bone fracture healing. The objectives of our study were to determine whether low-intensity pulsed US could promote traumatic vertebral fracture healing and to explore its inner mechanisms. METHODS: A rat model of traumatic vertebral fracture was created and treated with low-intensity pulsed US after surgery...
January 24, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/29359863/dose-and-timing-of-n-cadherin-mimetic-peptides-regulate-msc-chondrogenesis-within-hydrogels
#20
Mi Y Kwon, Sebastián L Vega, William M Gramlich, Minwook Kim, Robert L Mauck, Jason A Burdick
The transmembrane glycoprotein N-cadherin (NCad) mediates cell-cell interactions found during mesenchymal condensation and chondrogenesis. Here, NCad-derived peptides (i.e., HAV) are incorporated into hyaluronic acid (HA) hydrogels with encapsulated mesenchymal stem cells (MSCs). Since the dose and timing of NCad signaling are dynamic, HAV peptide presentation is tuned via alterations in peptide concentration and incorporation of an ADAM10-cleavable domain between the hydrogel and the HAV motif, respectively...
January 23, 2018: Advanced Healthcare Materials
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