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https://www.readbyqxmd.com/read/28819120/dual-delivery-of-fgf-2-ctgf-from-silk-fibroin-plcl-peo-coaxial-fibers-enhances-msc-proliferation-and-fibrogenesis
#1
Ruodan Xu, Huiling Zhao, Hanif Muhammad, Mingdong Dong, Flemming Besenbacher, Menglin Chen
The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolactone)-polyethylene oxide (SF/PLCL-PEO) enhanced fibrogenic lineage differentiation of MSCs. The core-shell structure was confirmed by transmission electron microscopy (TEM), fluorescence microscopy and attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28817971/effects-of-solid-acellular-type-i-iii-collagen-biomaterials-on-in-vitro-and-in-vivo-chondrogenesis-of-mesenchymal-stem-cells
#2
Liang Gao, Patrick Orth, Magali Cucchiarini, Henning Madry
Type-I/III collagen membranes are advocated for clinical use in articular cartilage repair as being able of inducing chondrogenesis, a technique termed autologous matrix-induced chondrogenesis (AMIC). Area covered: The current in vitro and translational in vivo evidence for chondrogenic effects of solid acellular type-I/III collagen biomaterials. Expert commentary: In vitro, mesenchymal stem cells (MSCs) adhere to the fibers of the type-I/III collagen membrane. No in vitro study provides evidence that a type-I/III collagen matrix alone may induce chondrogenesis...
August 18, 2017: Expert Review of Medical Devices
https://www.readbyqxmd.com/read/28810001/deferoxamine-synergizes-with-transforming-growth-factor-%C3%AE-signaling-in-chondrogenesis
#3
Zheng Huang, Guangxu He, Yanke Huang
Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, is an epidemic disease that affects millions of people worldwide. Despite extensive recent work on the cellular biology of osteoarthritis, the precise mechanisms involved are still poorly understood and there is no effective treatment for this disease. The role of transforming growth factor-beta (TGF-β) in promoting chondrogenesis and inducing the expression of cartilage-specific extracellular matrix molecules to form cartilage is well-established...
August 14, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28808630/systematic-review-on-the-use-of-autologous-matrix-induced-chondrogenesis-for-the-repair-of-articular-cartilage-defects-in-patients
#4
Nafisa Shaikh, Matthew K T Seah, Wasim S Khan
AIM: To systematically review the results of studies looking at autologous matrix-induced chondrogenesis (AMIC) in humans. METHODS: A literature search was performed, adhering to the PRISMA guidelines, to review any studies using such techniques in humans. Our initial search retrieved 297 articles listed on MEDLINE, Google Scholar, CINHal and EMBASE. From these studies, 15 studies meeting the eligibility criteria were selected and formed the basis of our systematic review...
July 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/28807899/pitx1-directly-modulates-the-core-limb-development-program-to-implement-hindlimb-identity
#5
Stephen Nemec, Maëva Luxey, Deepak Jain, Aurélie Huang Sung, Tomi Pastinen, Jacques Drouin
Forelimbs (FL) and hindlimbs (HL) develop complex musculoskeletal structures that rely on the deployment of a conserved developmental program. Pitx1, a transcription factor gene with expression restricted to HL and absent from FL, plays an important role in generating HL features. The genomic mechanisms by which Pitx1 effects HL identity remain poorly understood, however. Here, we use expression profiling and analysis of direct Pitx1 targets to characterize the HL- and FL-restricted genetic programs and situate the Pitx1-dependent gene network within the context of limb-specific gene regulation...
August 14, 2017: Development
https://www.readbyqxmd.com/read/28800889/spatio-temporal-expression-patterns-of-wnt-signaling-pathway-during-the-development-of-temporomandibular-condylar-cartilage
#6
Kan Chen, Huixin Quan, Gang Chen, Di Xiao
There is a growing body of evidence supporting the involvement of the Wnt signaling pathway in various aspects of skeletal and joint development; however, it is unclear whether it is involved in the process of temporomandibular joint development. In order to clarify this issue, we examined the spatio-temporal distribution of mRNAs and proteins of the Wnt family during the formation of the mandibular condylar cartilage at the prenatal and postnatal stages. An in situ hybridization test revealed no mRNAs of β-catenin and Axin2 during early mesenchymal condensation; the ligands surveyed in this study (including Wnt-4, 5a, and 9a) were clearly detected at various ranges of expression, mainly in the condylar blastema and later distinct cartilaginous layers...
August 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28800402/extracellular-matrix-cartilage-allograft-and-particulate-cartilage-allograft-for-osteochondral-lesions-of-the-knee-and-ankle-joints-a-systematic-review
#7
Dexter Seow, Youichi Yasui, Eoghan T Hurley, Andrew W Ross, Christopher D Murawski, Yoshiharu Shimozono, John G Kennedy
BACKGROUND: Extracellular matrix cartilage allografts (EMCAs) and particulate cartilage allografts (PCAs) are relatively new biologics that may improve the quality of cartilage regeneration after bone marrow stimulation. The increasing popularity of these novel biologics in the treatment of osteochondral lesions (OCLs) of the knee and ankle joints prompts a systematic evaluation of their efficacies. PURPOSE: The purpose of this systematic review was to clarify the effectiveness of EMCAs and PCAs on cartilage regeneration...
August 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28782725/the-bio-in-the-ink-cartilage-regeneration-with-bioprintable-hydrogels-and-articular-cartilage-derived-progenitor-cells
#8
Riccardo Levato, William R Webb, Iris A Otto, Anneloes Mensinga, Yadan Zhang, Mattie van Rijen, René van Weeren, Ilyas M Khan, Jos Malda
Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration...
August 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28770483/evaluation-of-reproducibility-of-the-mocart-score-in-patients-with-osteochondral-lesions-of-the-talus-repaired-using-the-autologous-matrix-induced-chondrogenesis-technique
#9
Domenico Albano, Nicolò Martinelli, Alberto Bianchi, Antonino Giacalone, Luca Maria Sconfienza
PURPOSE: To evaluate the applicability and reproducibility of magnetic resonance observation of cartilage repair tissue (MOCART) score for morphological evaluation of osteochondral lesions of the talus (OLT) repaired using autologous matrix-induced chondrogenesis (AMIC) technique. METHODS: Two radiologists (R1-R2) and two orthopaedists (O1-O2) independently reviewed 26 ankle MRIs performed on 13 patients (6 females; age: 38.9 ± 15.9, 14-63) with OLT repaired using AMIC...
August 2, 2017: La Radiologia Medica
https://www.readbyqxmd.com/read/28770354/thrap3-interacts-with-and-inhibits-the-transcriptional-activity-of-sox9-during-chondrogenesis
#10
Takashi Sono, Haruhiko Akiyama, Shigenori Miura, Jian Min Deng, Chisa Shukunami, Yuji Hiraki, Yu Tsushima, Yoshiaki Azuma, Richard R Behringer, Shuichi Matsuda
Sex-determining region Y (Sry)-box (Sox)9 is required for chondrogenesis as a transcriptional activator of genes related to chondrocyte proliferation, differentiation, and cartilage-specific extracellular matrix. Although there have been studies investigating the Sox9-dependent transcriptional complexes, not all their components have been identified. In the present study, we demonstrated that thyroid hormone receptor-associated protein (THRAP)3 is a component of a SOX9 transcriptional complex by liquid chromatography mass spectrometric analysis of FLAG-tagged Sox9-binding proteins purified from FLAG-HA-tagged Sox9 knock-in mice...
August 2, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/28769981/human-suprapatellar-fat-pad-derived-mesenchymal-stem-cells-induce-chondrogenesis-and-cartilage-repair-in-a-model-of-severe-osteoarthritis
#11
Ignacio Muñoz-Criado, Jose Meseguer-Ripolles, Maravillas Mellado-López, Ana Alastrue-Agudo, Richard J Griffeth, Jerónimo Forteza-Vila, Ramón Cugat, Montserrat García, Victoria Moreno-Manzano
Cartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown to restore degenerated cartilage; however, regenerative differences of ASC would depend on the source of adipose tissue. The infra- and suprapatellar fat pads surrounding the knee offer a potential autologous source of ASC for patients after complete joint substitution...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28768930/curcumin-inhibits-chondrocyte-hypertrophy-of-mesenchymal-stem-cells-through-ihh-and-notch-signaling-pathways
#12
Zhen Cao, Ce Dou, Shiwu Dong
Using tissue engineering technique to repair cartilage damage caused by osteoarthritis is a promising strategy. However, the regenerated tissue usually is fibrous cartilage, which has poor mechanical characteristics compared to hyaline cartilage. Chondrocyte hypertrophy plays an important role in this process. Thus, it is very important to find out a suitable way to maintain the phenotype of chondrocytes and inhibit chondrocyte hypertrophy. Curcumin deriving from turmeric was reported with anti-inflammatory and anti-tumor pharmacological effects...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28763118/mechanical-stimulation-of-mesenchymal-stem-cells-implications-for-cartilage-tissue-engineering
#13
REVIEW
Niamh Fahy, Mauro Alini, Martin J Stoddart
Articular cartilage is a load-bearing tissue playing a crucial mechanical role in diarthrodial joints, facilitating joint articulation, and minimizing wear. The significance of biomechanical stimuli in the development of cartilage and maintenance of chondrocyte phenotype in adult tissues has been well documented. Furthermore, dysregulated loading is associated with cartilage pathology highlighting the importance of mechanical cues in cartilage homeostasis. The repair of damaged articular cartilage resulting from trauma or degenerative joint disease poses a major challenge due to a low intrinsic capacity of cartilage for self-renewal, attributable to its avascular nature...
August 1, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28761049/improving-cartilage-phenotype-from-differentiated-pericytes-in-tunable-peptide-hydrogels
#14
Enateri V Alakpa, Vineetha Jayawarna, Karl E V Burgess, Christopher C West, Bruno Péault, Rein V Ulijn, Matthew J Dalby
Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759313/glycosylation-of-dmp1-is-essential-for-chondrogenesis-of-condylar-cartilage
#15
Y Weng, Y Liu, H Du, L Li, B Jing, Q Zhang, X Wang, Z Wang, Y Sun
The mandibular condylar cartilage (MCC) shoulders force for the subchondral bone during mastication. The cartilage matrix contains various large molecules, such as type I, II, and X collagens and proteoglycans (PGs), which jointly play essential roles in maintaining cartilage characteristics. PGs play key roles in maintaining the elasticity of cartilage and providing a cushion against mastication forces. In addition to the well-known PGs, DMP1-PG, which is the PG form of dentin matrix protein 1 (DMP1), is a newly identified PG...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28758906/activin-a-enhances-mtor-signaling-to-promote-aberrant-chondrogenesis-in-fibrodysplasia-ossificans-progressiva
#16
Kyosuke Hino, Kazuhiko Horigome, Megumi Nishio, Shingo Komura, Sanae Nagata, Chengzhu Zhao, Yonghui Jin, Koichi Kawakami, Yasuhiro Yamada, Akira Ohta, Junya Toguchida, Makoto Ikeya
Fibrodysplasia ossificans progressiva (FOP) is a rare and intractable disease characterized by extraskeletal bone formation through endochondral ossification. Patients with FOP harbor point mutations in ACVR1, a type I receptor for BMPs. Although mutated ACVR1 (FOP-ACVR1) has been shown to render hyperactivity in BMP signaling, we and others have uncovered a mechanism by which FOP-ACVR1 mistransduces BMP signaling in response to Activin-A, a molecule that normally transduces TGF-β signaling. Although Activin-A evokes enhanced chondrogenesis in vitro and heterotopic ossification (HO) in vivo, the underlying mechanisms have yet to be revealed...
July 31, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28756737/mimicking-the-biochemical-and-mechanical-extracellular-environment-of-the-endochondral-ossification-process-to-enhance-the-in-vitro-mineralization-potential-of-human-mscs
#17
Fiona E Freeman, Jessica Schiavi, Meadhbh A Brennan, Peter Owens, Pierre Layrolle, Laoise McNamara
Chondrogenesis and mechanical stimulation of the cartilage template, are essential for bone formation via the endochondral ossification process in vivo. Recent studies have demonstrated that in vitro regeneration strategies that mimic these aspects separately, either chondrogenesis or mechanical stimulation, can promote mineralization to a certain extent both in vitro and in vivo. However, to date no study has sought to incorporate both the formation of the cartilage template and the application of mechanical stimulation simultaneously to induce osteogenesis...
July 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28746404/pitx1-determines-characteristic-hindlimb-morphologies-in-cartilage-micromass-culture
#18
Natalie C Butterfield, Chen Qian, Malcolm P O Logan
The shapes of homologous skeletal elements in the vertebrate forelimb and hindlimb are distinct, with each element exquisitely adapted to their divergent functions. Many of the signals and signalling pathways responsible for patterning the developing limb bud are common to both forelimb and hindlimb. How disparate morphologies are generated from common signalling inputs during limb development remains poorly understood. We show that, similar to what has been shown in the chick, characteristic differences in mouse forelimb and hindlimb cartilage morphology are maintained when chondrogenesis proceeds in vitro away from the endogenous limb bud environment...
2017: PloS One
https://www.readbyqxmd.com/read/28737162/silk-fiber-reinforcement-modulates-in-vitro-chondrogenesis-in-3d-composite-scaffolds
#19
Yogendra Pratap Singh, Mimi Adhikary, Nandana Bhardwaj, Bibhas Kumar Bhunia, Biman B Mandal
The limited self-regenerative capacity of adult cartilage has steered the upsurge in tissue engineered replacements to combat the problem of osteoarthritis. In the present study, the potential of fiber-reinforced silk composites from mulberry (Bombyx mori) and non-mulberry (Antheraea assamensis) silk has been investigated for cartilage tissue engineering. The fabricated composites were physico-chemically characterized and analyzed for cellular viability, proliferation, extracellular matrix formation and immunocompatibility...
July 24, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28735352/long-noncoding-rna-expression-profiles-in-chondrogenic-and-hypertrophic-differentiation-of-mouse-mesenchymal-stem-cells
#20
Zhen Cao, Song Huang, Jianmei Li, Yun Bai, Ce Dou, Chuan Liu, Fei Kang, Xiaoshan Gong, Haibin Ding, Tianyong Hou, Shiwu Dong
Long noncoding RNAs (lncRNAs) are important regulators for a variety of biological processes. Chondrogenic differentiation of mesenchymal stem cells (MSCs) is a crucial stage in chondrogenesis while chondrocyte hypertrophy is related to endochondral ossification and osteoarthritis. However, the effects of lncRNAs on chondrogenic and hypertrophic differentiation of mouse MSCs are unclear. To explore the potential mechanisms of lncRNAs during chondrogenesis and chondrocyte hypertrophy, microarray was performed to investigate the expression profiles of lncRNA and mRNA in MSCs, pre-chondrocytes, and hypertrophic chondrocytes...
July 22, 2017: Functional & Integrative Genomics
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