keyword
MENU ▼
Read by QxMD icon Read
search

chondrogenesis

keyword
https://www.readbyqxmd.com/read/28218494/influence-of-oxygen-levels-on-chondrogenesis-of-porcine-mesenchymal-stem-cells-cultured-in-polycaprolactone-scaffolds
#1
Joaquin Rodenas-Rochina, Daniel J Kelly, Jose Luis Gómez Ribelles, Myriam Lebourg
Chondrogenesis of Mesenchymal stem cells (MSCs) is known to be regulated by a number of environmental factors, including local oxygen levels. The hypothesis of this study is that the response of MSCs to hypoxia is dependent on the physical and chemical characteristics of the substrate used. The objective of this study was to explore how different modifications to polycaprolactone (PCL) scaffolds influenced the response of MSCs to hypoxia. PCL, PCL-hyaluronic acid (HA) and PCL-Bioglass® (BG) scaffolds were seeded with MSCs derived from bone marrow and cultured for 35 days under normoxic or low oxygen conditions, and the resulting biochemical properties of the MSC laden construct were assessed...
February 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28217849/how-long-bones-grow-children-mechanistic-paths-to-variation-in-human-height-growth
#2
REVIEW
Michelle Lampl, Meriah Schoen
OBJECTIVES: Eveleth and Tanner's descriptive documentation of worldwide variability in human growth provided evidence of the interaction between genetics and environment during development that has been foundational to the science of human growth. There remains a need, however, to describe the mechanistic foundations of variability in human height growth patterns. METHODS: A review of research documenting cellular activities at the endochondral growth plate aims to show how the unique microenvironment and cell functions during the sequential phases of the chondrocyte lifecycle affect long bone elongation, a fundamental source of height growth...
February 20, 2017: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/28213129/cyclic-compressive-stress-induced-scinderin-regulates-progress-of-developmental-dysplasia-of-the-hip
#3
Cheng-Long Wang, Hui Wang, Fei Xiao, Chuan-Dong Wang, Guo-Li Hu, Jun-Feng Zhu, Chao Shen, Bin Zuo, Yi-Min Cui, De Li, Yuan-Gao, Xiao-Ling Zhang, Xiao-Dong Chen
Developmental dysplasia of the hip (DDH) is a common musculoskeletal disorder characterized by a mismatch between acetabulum and femoral head. Mechanical force plays an important role during the occurrence and development of abnormities in acetabulum and femoral head. In this study, we established a mechanical force model named cyclic compressive stress (Ccs). To analyze the effect of Ccs on DDH, we detected special genes in chondrocytes and osteoblasts. Results showed that Ccs downregulated chondrogenesis of ADTC5 in a concentration-dependent manner...
February 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28210226/combination-of-collagen-based-scaffold-and-bioactive-factors-induces-adipose-derived-mesenchymal-stem-cells-chondrogenic-differentiation-in-vitro
#4
Giovanna Calabrese, Stefano Forte, Rosario Gulino, Francesco Cefalì, Elisa Figallo, Lucia Salvatorelli, Eugenia T Maniscalchi, Giuseppe Angelico, Rosalba Parenti, Massimo Gulisano, Lorenzo Memeo, Raffaella Giuffrida
Recently, multipotent mesenchymal stem cells (MSCs) have attracted much attention in the field of regenerative medicine due to their ability to give rise to different cell types, including chondrocytes. Damaged articular cartilage repair is one of the most challenging issues for regenerative medicine, due to the intrinsic limited capability of cartilage to heal because of its avascular nature. While surgical approaches like chondral autografts and allografts provide symptoms and function improvement only for a short period, MSC based stimulation therapies, like microfracture surgery or autologous matrix-induced chondrogenesis demonstrate to be more effective...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28194374/regenerative-potential-of-the-cartilaginous-tissue-in-mesenchymal-stem-cells-update-limitations-and-challenges
#5
Ivana Beatrice Mânica da Cruz, Antônio Lourenço Severo, Verônica Farina Azzolin, Luiz Filipe Machado Garcia, André Kuhn, Osvandré Lech
Advances in the studies with adult mesenchymal stem cells (MSCs) have turned tissue regenerative therapy into a promising tool in many areas of medicine. In orthopedics, one of the main challenges has been the regeneration of cartilage tissue, mainly in diarthroses. In the induction of the MSCs, in addition to cytodifferentiation, the microenvironmental context of the tissue to be regenerated and an appropriate spatial arrangement are extremely important factors. Furthermore, it is known that MSC differentiation is fundamentally determined by mechanisms such as cell proliferation (mitosis), biochemical-molecular interactions, movement, cell adhesion, and apoptosis...
January 2017: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/28193542/sulfated-hyaluronic-acid-hydrogels-with-retarded-degradation-and-enhanced-growth-factor-retention-promote-hmsc-chondrogenesis-and-articular-cartilage-integrity-with-reduced-hypertrophy
#6
Qian Feng, Sien Lin, Kunyu Zhang, Chaoqun Dong, Tianyi Wu, Heqin Huang, Xiaohui Yan, Li Zhang, Gang Li, Liming Bian
: Recently, hyaluronic acid (HA) hydrogels have been extensively researched for delivering cells and drugs to repair damaged tissues, particularly articular cartilage. However, the in vivo degradation of HA is fast, thus limiting the clinical translation of HA hydrogels. Furthermore, HA cannot bind proteins with high affinity because of the lack of negatively charged sulfate groups. In this study, we conjugated sulfate groups to HA. These sulfated HA exhibit significantly slower degradation by hyaluronidase compared to the wild type HA...
February 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28191761/angiogenic-potential-of-human-bone-marrow-derived-mesenchymal-stem-cells-in-chondrocyte-brick-enriched-constructs-promoted-stable-regeneration-of-craniofacial-cartilage
#7
Zhiye Li, Ruikai Ba, Zhifa Wang, Jianhua Wei, Yimin Zhao, Wei Wu
Craniofacial deformities caused by congenital defects or trauma remain challenges for clinicians, whereas current surgical interventions present limited therapeutic outcomes. Injection of bone marrow-derived mesenchymal stem cells (BMSCs) into the defect is highly desirable because such a procedure is microinvasive and grafts are more flexible to fill the lesions. However, preventing hypertrophic transition and morphological contraction remain significant challenges. We have developed an "all host derived" cell transplantation system composed of chondrocyte brick (CB)-enriched platelet-rich plasma (P) gel and BMSCs (B)...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28162996/identification-of-bone-marrow-derived-soluble-factors-regulating-human-mesenchymal-stem-cells-for-bone-regeneration
#8
Tsung-Lin Tsai, Wan-Ju Li
Maintaining properties of human bone marrow-derived mesenchymal stem cells (BMSCs) in culture for regenerative applications remains a great challenge. An emerging approach of constructing a culture environment mimicking the bone marrow niche to regulate BMSC activities has been developed. In this study, we have demonstrated a systematic approach to identify soluble factors of interest extracted from human bone marrow and used them in BMSC culture for tissue regeneration. We have found that lipocalin-2 and prolactin are key factors in bone marrow, involved in regulating BMSC activities...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28161663/is-there-a-cause-and-effect-relationship-between-physicochemical-properties-and-cell-behavior-of-alginate-based-hydrogel-obtained-after-sterilization
#9
Hao Yu, Ghislaine Cauchois, Jean-François Schmitt, Nicolas Louvet, Jean-Luc Six, Yun Chen, Rachid Rahouadj, Céline Huselstein
Alginate-based hydrogel scaffolds are widely used in the field of cartilage regeneration and repair. If the effect of autoclaving on the alginate powder is well known, it is not the same for the possible effects of the sterilization UV treatment on the properties of the hydrogel after polymerization. To select an effective sterilization treatment of alginate-based materials, one must find what are inter-relationship between the characteristics (chemical, physical and mechanical) of alginate-based hydrogel during sterilization, and what consequences have affected on cell behavior...
January 25, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28131943/hyaluronic-acid-facilitates-chondrogenesis-and-matrix-deposition-of-human-adipose-derived-mesenchymal-stem-cells-and-human-chondrocytes-co-cultures
#10
Elisabeth Amann, Paul Wolff, Ernst Breel, Martijn van Griensven, Elizabeth R Balmayor
: Clinical success on cartilage regeneration could be achieved by using available biomaterials and cell-based approaches. In this study, we have developed a composite gel based on collagen/hyaluronic acid (Coll-HA) as ideal, physiologically representative 3D support for in vitro chondrogenesis of human adipose mesenchymal stem cells (hAMSCs) co-cultured with human articular chondrocytes (hAC). The incorporation of hyaluronic acid (HA) attempted to provide an additional stimulus to the hAMSCs for chondrogenesis and extracellular matrix deposition...
January 25, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28123639/microrna-221-is-involved-in-the-regulation-of-osteoporosis-through-regulates-runx2-protein-expression-and-osteoblast-differentiation
#11
Yinquan Zhang, Yulei Gao, Lijun Cai, Fengning Li, Yi Lou, Ning Xu, Yifan Kang, Huilin Yang
INTRODUCTION: MicroRNAs (miRNAs) has emerged as important factors in osteogenesis and chondrogenesis. This study aimed to determine whether miR-221 is involved in the regulation of osteoporosis and its underlying mechanism. METHODS: Total RNA was extracted from fresh femoral neck trabecular bone from women undergoing hip replacement due to either osteoporotic fracture (OP group, n = 12) or osteoarthritis in the absence of osteoporosis (Control group, n = 12). Gene expression was quantified using TaqMan quantitative RT-PCR assays and protein production was determined by western blot analysis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28123350/the-effect-of-estradiol-on-the-growth-plate-chondrocytes-of-limb-and-spine-from-postnatal-mice-in-vitro-the-role-of-estrogen-receptor-and-estradiol-concentration
#12
Sheng Shi, Shuang Zheng, Xin-Feng Li, Zu-De Liu
Objectives: Skeletal development is a complex process. Little is known about the different response of limb or spine growth plate chondrocytes (LGP or SGP) to the estrogen level and the role of estrogen receptor (ER) during postnatal stage. Methods: LGP and SGP chondrocytes were isolated from 50 one-week mice and treated with different concentrations of 17β-estradiol. Cell viability was measured by cell counting kit-8 (CCK-8). The expression of collagen II and X were evaluated by real-time PCR and Western blotting...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28121025/distinctive-expression-pattern-of-cystathionine-%C3%AE-synthase-and-cystathionine-%C3%AE-lyase-identifies-mesenchymal-stromal-cells-transition-to-mineralizing-osteoblasts
#13
Laura Gambari, Gina Lisignoli, Elena Gabusi, Cristina Manferdini, Francesca Paolella, Anna Piacentini, Francesco Grassi
Mesenchymal stromal cells (MSCs) are key players in the repair or regeneration of the damaged bone tissue. However, heterogeneity exists between MSCs derived from different donors in their bone formation ability both in vitro and in vivo. The identification of markers defining MSCs with different functional phenotypes is fundamental to maximize their clinical potential. In our previous in vivo study, impaired expression in MSCs of cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), the two key enzymes in the catabolic pathway of homocysteine, was associated to decreased bone formation and to the onset of osteoporosis in mice...
January 25, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28116125/use-of-fgf-2-and-fgf-18-to-direct-bone-marrow-stromal-stem-cells-to-chondrogenic-and-osteogenic-lineages
#14
Cindy Shu, Susan M Smith, Christopher B Little, James Melrose
AIM: Intervertebral disc degeneration/low back pain is the number one global musculoskeletal condition in terms of disability and socioeconomic impact. MATERIALS & METHODS: Multipotent mesenchymal stem cells (MSCs) were cultured in micromass pellets ± FGF-2 or -18 up to 41 days, matrix components were immunolocalized and gene expression monitored by quantitative-reverse transcription PCR. RESULTS: Chondrogenesis occurred earlier in FGF-18 than FGF-2 cultures...
December 2016: Future Science OA
https://www.readbyqxmd.com/read/28109149/-indirect-coculture-of-human-infrapatellar-fat-pad-derived-stem-cells-with-osteoarthritic-chondrocytes-induces-their-chondrogenesis
#15
Junjun Yang, Cheng Chen, Liu Yang, Xiongbo Song, Wenbin Xie, Shu Huang, Baorong Liu
Objective To promote phenotype recovery of osteoarthritic articular chondrocytes (OACs) and induce chondrogenic differentiation of infrapatellar fat pad-derived stem cells (IPFPSCs) by indirectly coculturing these two types of cells. Methods The OACs and IPFPSCs were isolated and cultured in vitro. This experiment included single IPFPSCs group, single OACs group, and coculture group. After cells were cultured in vitro with chondrogenic medium for 21 days, the chondrocyte phenotypes were determined by HE staining (cell morphology), Alcian blue staining (glycosaminoglycan content) and immunofluorescence cytochemistry (collagen 1, collagen 2, collagen 3, aggrecan, SOX9)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28108777/a-randomized-controlled-trial-demonstrating-sustained-benefit-of-autologous-matrix-induced-chondrogenesis-over-microfracture-at-five%C3%A2-years
#16
Martin Volz, Jens Schaumburger, Hubert Frick, Joachim Grifka, Sven Anders
PURPOSE: Autologous Matrix-Induced Chondrogenesis (AMIC(®)) utilizing a type I/III collagen membrane was compared with microfracture (MFx) alone in focal cartilage lesions of the knee at one, two and five years. METHODS: Forty-seven patients (aged 37 ± 10 years, mean defect size 3.6 ± 1.6 cm(2)) were randomized and treated either with MFx, with sutured or glued AMIC(®) in a prospective multicentre clinical trial. RESULTS: After improvement for the first two years in all subgroups, a progressive and significant score degradation was observed in the MFx group, while all functional parameters remained stable for least five years in the AMIC(®) groups...
January 20, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28103242/genome-wide-binding-of-posterior-hoxa-d-transcription-factors-reveals-subgrouping-and-association-with-ctcf
#17
Ivana Jerković, Daniel M Ibrahim, Guillaume Andrey, Stefan Haas, Peter Hansen, Catrin Janetzki, Irene González Navarrete, Peter N Robinson, Jochen Hecht, Stefan Mundlos
Homeotic genes code for key transcription factors (HOX-TFs) that pattern the animal body plan. During embryonic development, Hox genes are expressed in overlapping patterns and function in a partially redundant manner. In vitro biochemical screens probing the HOX-TF sequence specificity revealed largely overlapping sequence preferences, indicating that co-factors might modulate the biological function of HOX-TFs. However, due to their overlapping expression pattern, high protein homology, and insufficiently specific antibodies, little is known about their genome-wide binding preferences...
January 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28088667/cell-laden-hydrogels-for-osteochondral-and-cartilage-tissue-engineering
#18
REVIEW
Jingzhou Yang, Yu Shrike Zhang, Kan Yue, Ali Khademhosseini
: Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue engineered artificial matrices that can replace the damaged regions and promote tissue regeneration. Hydrogels are emerging as a promising class of biomaterials for both soft and hard tissue regeneration. Many critical properties of hydrogels, such as mechanical stiffness, elasticity, water content, bioactivity, and degradation, can be rationally designed and conveniently tuned by proper selection of the material and chemistry...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28087486/enhanced-articular-cartilage-by-human-mesenchymal-stem-cells-in-enzymatically-mediated-transiently-rgds-functionalized-collagen-mimetic-hydrogels
#19
Paresh A Parmar, Jean-Philippe St-Pierre, Lesley W Chow, Christopher D Spicer, Violet Stoichevska, Yong Y Peng, Jerome A Werkmeister, John A M Ramshaw, Molly M Stevens
: Recapitulation of the articular cartilage microenvironment for regenerative medicine applications faces significant challenges due to the complex and dynamic biochemical and biomechanical nature of native tissue. Towards the goal of biomaterial designs that enable the temporal presentation of bioactive sequences, recombinant bacterial collagens such as Streptococcal collagen-like 2 (Scl2) proteins can be employed to incorporate multiple specific bioactive and biodegradable peptide motifs into a single construct...
January 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28076994/adapted-dexamethasone-delivery-polyethylene-oxide-and-poly-%C3%A9-caprolactone-construct-promote-mesenchymal-stem-cells-chondrogenesis
#20
Armaghan Ghiaee, Faeze Pournaqi, Saeid Vakilian, Abdolah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi
Issues associated with tissue transplantation and shortage of donors has always been a concern, whereas tissue engineering has provided the hopeful opportunities. The aim of this study was to investigate the chondrogenic differentiation potential of mesenchymal stem cells (MSCs) in the presence of embedded Dexamethasone into electrospun Poly(ethyleneoxide) nanofibers composited with Poly(ɛ-caprolactone) nanofibers. Electrospun-fabricated scaffolds were characterized by SEM, tensile, contact angle, release profile, MTT assay, and chonderogenic differentiation of stem cells...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
keyword
keyword
110153
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"