keyword
MENU ▼
Read by QxMD icon Read
search

Fission yeast

keyword
https://www.readbyqxmd.com/read/28218250/chromatin-remodeller-fun30-fft3-induces-nucleosome-disassembly-to-facilitate-rna-polymerase-ii-elongation
#1
Junwoo Lee, Eun Shik Choi, Hogyu David Seo, Keunsoo Kang, Joshua M Gilmore, Laurence Florens, Michael P Washburn, Joonho Choe, Jerry L Workman, Daeyoup Lee
Previous studies have revealed that nucleosomes impede elongation of RNA polymerase II (RNAPII). Recent observations suggest a role for ATP-dependent chromatin remodellers in modulating this process, but direct in vivo evidence for this is unknown. Here using fission yeast, we identify Fun30(Fft3) as a chromatin remodeller, which localizes at transcribing regions to promote RNAPII transcription. Fun30(Fft3) associates with RNAPII and collaborates with the histone chaperone, FACT, which facilitates RNAPII elongation through chromatin, to induce nucleosome disassembly at transcribing regions during RNAPII transcription...
February 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28208632/cross-talk-between-dnmt2-dependent-trna-methylation-and-queuosine-modification
#2
REVIEW
Ann E Ehrenhofer-Murray
Enzymes of the Dnmt2 family of methyltransferases have yielded a number of unexpected discoveries. The first surprise came more than ten years ago when it was realized that, rather than being DNA methyltransferases, Dnmt2 enzymes actually are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp. The second unanticipated finding was our recent discovery of a nutritional regulation of Dnmt2 in the fission yeast Schizosaccharomyces pombe. Significantly, the presence of the nucleotide queuosine in tRNAAsp strongly stimulates Dnmt2 activity both in vivo and in vitro in S...
February 10, 2017: Biomolecules
https://www.readbyqxmd.com/read/28202541/coordinate-regulation-of-yeast-sterol-regulatory-element-binding-protein-srebp-and-mga2-transcription-factors
#3
Risa Burr, Emerson V Stewart, Peter J Espenshade
The Mga2 and Sre1 transcription factors regulate oxygen-responsive lipid homeostasis in the fission yeast Schizosaccharomyces pombe in a manner analogous to the mammalian Sterol Regulatory Element-Binding Protein transcription factors SREBP-1 and SREBP-2. Mga2 and SREBP-1 regulate triacylglycerol and glycerophospholipid synthesis, while Sre1 and SREBP-2 regulate sterol synthesis. In mammals, a shared activation mechanism allows for coordinate regulation of SREBP-1 and SREBP-2. In contrast, distinct pathways activate fission yeast Mga2 and Sre1...
February 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28199302/untimely-expression-of-gametogenic-genes-in-vegetative-cells-causes-uniparental-disomy
#4
H Diego Folco, Venkata R Chalamcharla, Tomoyasu Sugiyama, Gobi Thillainadesan, Martin Zofall, Vanivilasini Balachandran, Jothy Dhakshnamoorthy, Takeshi Mizuguchi, Shiv I S Grewal
Uniparental disomy (UPD), in which an individual contains a pair of homologous chromosomes originating from only one parent, is a frequent phenomenon that is linked to congenital disorders and various cancers. UPD is thought to result mostly from pre- or post-zygotic chromosome missegregation. However, the factors that drive UPD remain unknown. Here we use the fission yeast Schizosaccharomyces pombe as a model to investigate UPD, and show that defects in the RNA interference (RNAi) machinery or in the YTH domain-containing RNA elimination factor Mmi1 cause high levels of UPD in vegetative diploid cells...
February 15, 2017: Nature
https://www.readbyqxmd.com/read/28193734/mitochondrial-depolarization-in-yeast-zygotes-inhibits-clonal-expansion-of-selfish-mtdna
#5
Iuliia E Karavaeva, Sergey A Golyshev, Ekaterina A Smirnova, Svyatoslav S Sokolov, Fedor F Severin, Dmitry A Knorre
Non-identical copies of mitochondrial DNA (mtDNA) compete with each other within a cell and the result depends on their replication rates. Using yeast Saccharomyces cerevisiae cells as a model, we studied the effects of mitochondrial inhibitors on the competition between wild type mtDNA and mutant selfish mtDNA in heteroplasmic zygotes. We found that decreasing mitochondrial transmembrane potential by adding uncouplers or valinomycin changes the competition outcomes in favor of the wild type mtDNA. This effect was significantly lower in the cells with disrupted mitochondria fission or repressed autophagy-related genes ATG8, ATG32 or ATG33 implying that heteroplasmic zygotes activate mitochondrial degradation in response to the depolarization...
February 13, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28191457/the-copper-transport-associated-protein-ctr4-can-form-prion-like-epigenetic-determinants-in-schizosaccharomyces-pombe
#6
Theodora Sideri, Yoko Yashiroda, David A Ellis, María Rodríguez-López, Minoru Yoshida, Mick F Tuite, Jürg Bähler
Prions are protein-based infectious entities associated with fatal brain diseases in animals, but also modify a range of host-cell phenotypes in the budding yeast, Saccharomyces cerevisiae. Many questions remain about the evolution and biology of prions. Although several functionally distinct prion-forming proteins exist in S. cerevisiae, [HET-s] of Podospora anserina is the only other known fungal prion. Here we investigated prion-like, protein-based epigenetic transmission in the fission yeast Schizosaccharomyces pombe...
January 2017: Microbial Cell
https://www.readbyqxmd.com/read/28180297/fission-yeast-stn1-is-crucial-for-semi-conservative-replication-at-telomeres-and-subtelomeres
#7
Masahiro Takikawa, Yusuke Tarumoto, Fuyuki Ishikawa
No abstract text is available yet for this article.
February 17, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28178520/identification-of-a-sgo2-dependent-but-mad2-independent-pathway-controlling-anaphase-onset-in-fission-yeast
#8
John C Meadows, Theresa C Lancaster, Graham J Buttrick, Alicja M Sochaj, Liam J Messin, Maria Del Mar Mora-Santos, Kevin G Hardwick, Jonathan B A Millar
The onset of anaphase is triggered by activation of the anaphase-promoting complex/cyclosome (APC/C) following silencing of the spindle assembly checkpoint (SAC). APC/C triggers ubiquitination of Securin and Cyclin B, which leads to loss of sister chromatid cohesion and inactivation of Cyclin B/Cdk1, respectively. This promotes relocalization of Aurora B kinase and other components of the chromosome passenger complex (CPC) from centromeres to the spindle midzone. In fission yeast, this is mediated by Clp1 phosphatase-dependent interaction of CPC with Klp9/MKLP2 (kinesin-6)...
February 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28178334/a-novel-mutual-information-based-boolean-network-inference-method-from-time-series-gene-expression-data
#9
Shohag Barman, Yung-Keun Kwon
BACKGROUND: Inferring a gene regulatory network from time-series gene expression data in systems biology is a challenging problem. Many methods have been suggested, most of which have a scalability limitation due to the combinatorial cost of searching a regulatory set of genes. In addition, they have focused on the accurate inference of a network structure only. Therefore, there is a pressing need to develop a network inference method to search regulatory genes efficiently and to predict the network dynamics accurately...
2017: PloS One
https://www.readbyqxmd.com/read/28176784/innovation-and-constraint-leading-to-complex-multicellularity-in-the-ascomycota
#10
Tu Anh Nguyen, Ousmane H Cissé, Jie Yun Wong, Peng Zheng, David Hewitt, Minou Nowrousian, Jason E Stajich, Gregory Jedd
The advent of complex multicellularity (CM) was a pivotal event in the evolution of animals, plants and fungi. In the fungal Ascomycota, CM is based on hyphal filaments and arose in the Pezizomycotina. The genus Neolecta defines an enigma: phylogenetically placed in a related group containing mostly yeasts, Neolecta nevertheless possesses Pezizomycotina-like CM. Here we sequence the Neolecta irregularis genome and identify CM-associated functions by searching for genes conserved in Neolecta and the Pezizomycotina, which are absent or divergent in budding or fission yeasts...
February 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28168604/the-natural-product-osthole-attenuates-yeast-growth-by-extensively-suppressing-the-gene-expressions-of-mitochondrial-respiration-chain
#11
Zhe Wang, Yan Shen
The fast growing evidences have indicated that the natural product osthole is a promising drug candidate for fighting several serious human diseases, for example, cancer and inflammation. However, the mode-of-action (MoA) of osthole remains largely incomplete. In this study, we investigated the growth inhibition activity of osthole using fission yeast as a model, with the goal of understanding the osthole's mechanism of action, especially from the molecular level. Microarray analysis indicated that osthole has significant impacts on gene transcription levels (In total, 214 genes are up-regulated, and 97 genes are down-regulated)...
February 7, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28162898/sin-dependent-dissociation-of-the-sad-kinase-cdr2-from-the-cell-cortex-resets-the-division-plane
#12
Sergio A Rincon, Miguel Estravis, Florent Dingli, Damarys Loew, Phong T Tran, Anne Paoletti
Proper division plane positioning is crucial for faithful chromosome segregation but also influences cell size, position, or fate [1]. In fission yeast, medial division is controlled through negative signaling by the cell tips during interphase and positive signaling by the centrally placed nucleus at mitotic entry [2-4]: the cell geometry network (CGN), controlled by the inhibitory cortical gradient of the DYRK kinase Pom1 emanating from the cell tips, first promotes the medial localization of cytokinetic ring precursors organized by the SAD kinase Cdr2 to pre-define the division plane [5-8]; then, massive nuclear export of the anillin-like protein Mid1 at mitosis entry confirms or readjusts the division plane according to nuclear position and triggers the assembly of a medial contractile ring [5, 9-11]...
February 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28160081/ecl1-is-a-zinc-binding-protein-involved-in-the-zinc-limitation-dependent-extension-of-chronological-life-span-in-fission-yeast
#13
Takafumi Shimasaki, Hokuto Ohtsuka, Chikako Naito, Kenko Azuma, Takeshi Tenno, Hidekazu Hiroaki, Hiroshi Murakami, Hirofumi Aiba
Overexpression of Ecl1-family genes (ecl1 (+), ecl2 (+), and ecl3 (+)) results in the extension of the chronological life span in Schizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein...
February 3, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28159842/direct-visualization-of-rna-dna-primer-removal-from-okazaki-fragments-provides-support-for-flap-cleavage-and-exonucleolytic-pathways-in-eukaryotic-cells
#14
Bochao Liu, Jiazhi Hu, Jingna Wang, Daochun Kong
During DNA replication in eukaryotic cells, short single-stranded DNA segments known as Okazaki fragments are first synthesized on the lagging strand. The Okazaki fragments originate from ~35 nt long RNA-DNA primers. After Okazaki fragment synthesis, these primers must be removed to allow fragment joining into a continuous lagging strand. To date, the models of enzymatic machinery that removes the RNA-DNA primers has come almost exclusively from biochemical reconstitution studies and some genetic interaction assays, and there is little direct evidence to confirm these models...
February 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28148853/large-scale-immunoprecipitation-from-fission-yeast-cell-extracts
#15
Agnes Grallert, Iain M Hagan
We outline immunoprecipitation (IP) procedures to isolate the large quantities of a molecule of interest that are required to identify posttranslational modifications (PTMs) in subsequent targeted mass spectrometry analysis. In situ denaturation by trichloroacetic acid precipitation inhibits the activities of modifying enzymes that could alter the PTM profile to preserve the PTMs on a target of interest throughout the precipitation step. In contrast, isolation of the same molecule with the nondenaturing variation on this IP procedure can maintain associations with partner molecules whose PTMs can also be mapped, albeit with the caveat that modifications could have occurred during the extended IP period...
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148852/small-scale-immunoprecipitation-from-fission-yeast-cell-extracts
#16
Agnes Grallert, Iain M Hagan
We describe procedures for the immunoprecipitation (IP) of a molecule of interest from cell extracts under native or denaturing conditions. The methods are equally effective with antibodies that directly recognize the molecule of interest and those that recognize a generic peptide "epitope tag" that has been fused to sequences encoding the gene of interest. The diverse chemistry of intermolecular interactions and enzymatic activities means that a range of different buffer conditions must be assessed empirically to identify optimal conditions for the study of a specific target/complex in a particular assay...
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28143796/clr4-specificity-and-catalytic-activity-beyond-h3k9-methylation
#17
Denis Kusevic, Srikanth Kudithipudi, Nahid Iglesias, Danesh Moazed, Albert Jeltsch
In fission yeast, the catalytic activity of the protein lysine methyltransferase (PKMT) Clr4, the sole homolog of the mammalian SUV39H1 and SUV39H2 enzymes, majorly contributes to the formation of heterochromatin. The enzyme introduces histone 3 lysine 9 (H3K9) di- and tri-methylation, a central heterochromatic histone modification, and later it was also found to methylate the Mlo3 protein, which has a role in heterochromatin formation as well. Herein, we have investigated the substrate specificity of Clr4 using custom made mutational scanning peptide arrays...
January 28, 2017: Biochimie
https://www.readbyqxmd.com/read/28142187/spatial-regulation-of-the-kh-domain-rna-binding-protein-rnc1-mediated-by-a-crm1-independent-nuclear-export-system-in-schizosaccharomyces-pombe
#18
Ryosuke Satoh, Yasuhiro Matsumura, Akitomo Tanaka, Makoto Takada, Yuna Ito, Kanako Hagihara, Masahiro Inari, Ayako Kita, Akira Fukao, Toshinobu Fujiwara, Shinya Hirai, Tokio Tani, Reiko Sugiura
RNA-binding proteins (RBPs) play important roles in the posttranscriptional regulation of gene expression, including mRNA stability, transport, and translation. Fission yeast rnc1(+) encodes a K Homology (KH)-type RBP, which binds and stabilizes the Pmp1 MAPK phosphatase mRNA thereby suppressing the Cl(-) hypersensitivity of calcineurin deletion and MAPK signaling mutants. Here, we analyzed the spatial regulation of Rnc1 and discovered a putative nuclear export signal (NES)Rnc1 , which dictates the cytoplasmic localization of Rnc1 in a Crm1-independent manner...
January 31, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28139976/an-aging-independent-replicative-lifespan-in-a-symmetrically-dividing-eukaryote
#19
Eric C Spivey, Stephen K Jones, James R Rybarski, Fatema A Saifuddin, Ilya J Finkelstein
The replicative lifespan (RLS) of a cell-defined as the number of cell divisions before death-has informed our understanding of the mechanisms of cellular aging. However, little is known about aging and longevity in symmetrically dividing eukaryotic cells because most prior studies have used budding yeast for RLS studies. Here, we describe a multiplexed fission yeast lifespan micro-dissector (multFYLM) and an associated image processing pipeline for performing high-throughput and automated single-cell micro-dissection...
January 31, 2017: ELife
https://www.readbyqxmd.com/read/28137950/many-roads-to-symmetry-breaking-molecular-mechanisms-and-theoretical-models-of-yeast-cell-polarity
#20
Andrew B Goryachev, Marcin Leda
Mathematical modeling has been instrumental in identifying common principles of cell polarity across diverse systems. These principles include positive feedback loops that are required to destabilize a spatially uniform state of the cell. The conserved small G-protein Cdc42 is a master regulator of eukaryotic cellular polarization. Here we discuss recent developments in studies of Cdc42 polarization in budding and fission yeasts and demonstrate that models describing symmetry-breaking polarization can be classified into six minimal classes based on the structure of positive feedback loops that activate and localize Cdc42...
February 1, 2017: Molecular Biology of the Cell
keyword
keyword
11013
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"