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https://www.readbyqxmd.com/read/28427494/characterization-and-expression-analysis-of-peroxiredoxin-genes-in-nnk-induced-v79-cells
#1
Gui Qin Shi, Wen Shan Zhou, Meng Li, Fei Ren, Ya Wei Han
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent and prevalent nitrosamine procarcinogen found in cigarette smoke. The aim of this work is to study alterations in peroxiredoxin (Prx) expression induced by NNK during carcinogenesis. Characterization of Prx genes from hamster was performed using bioinformatics. V79 cells were induced with different concentrations of NNK (0.1-0.4 mg/mL), and the expression levels of six Prx genes (Prx1-Prx6) were measured by qRT-PCR 24 h following NNK treatment...
March 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28410843/left-ventricular-hypertrophy-does-not-prevent-heart-failure-in-experimental-hypertension
#2
H Hernán Gómez Llambí, G Cao, M Donato, D Suárez, G Ottaviano, A Müller, B Buchholz, R Gelpi, M Otero-Losada, J Milei
BACKGROUND: Left ventricular hypertrophy (LVH) secondary to hypertension has been accepted to prevent heart failure (HF) while paradoxically increasing cardiovascular morbi-mortality. OBJECTIVES: To evaluate whether antihypertensive treatment inhibits LVH, restores beta-adrenergic response and affects myocardial oxidative metabolism. METHODS: Ninety spontaneously hypertensive rats (SHR) were distributed into groups and treated (mg/kg, p.o...
March 27, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28402171/tethered-bone-morphogenetic-protein-2-onto-sulfonated-polyrotaxane-based-surfaces-promotes-osteogenic-differentiation-of-mc3t3-e1-cells
#3
Yoshinori Arisaka, Nobuhiko Yui
Sulfonated-polyrotaxane (PRX)-coated surfaces with tethered bone morphogenetic protein-2 (BMP-2) were designed for inducing osteogenic differentiation of MC3T3-E1 cells (preosteoblasts). Sulfonated-PRX triblock copolymers composed of sulfopropyl ether-modified α-cyclodextrins (α-CDs) threaded onto a poly(ethylene glycol) (PEG) chain as a PRX segment and poly(benzyl methacrylate) (PBzMA) at both terminals of the PEG as anchoring segments were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization...
April 20, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28392436/an-oral-administration-of-a-recombinant-anti-tnf-fusion-protein-is-biologically-active-in-the-gut-promoting-regulatory-t-cells-results-of-a-phase-i-clinical-trial-using-a-novel-oral-anti-tnf-alpha-based-therapy
#4
Einat Almon, Tawfik Khoury, Ariel Drori, Svetlana Gingis-Velitski, Sari Alon, Raul Chertkoff, Mordechai Mushkat, Yoseph Shaaltiel, Yaron Ilan
BACKGROUND: An orally administered BY-2 plant cell-expressed recombinant anti-TNF fusion protein (PRX-106) consists of the soluble form of the human TNF receptor (TNFR) fused to the Fc component of a human IgG1 domain. Aim This study aim at determining the safety and the immune modulatory effect of an oral administration of PRX-106 in humans. METHODS: Three different doses (2, 8 or 16mg/day) of PRX-106 were orally administered for five consecutive days in 14 healthy volunteered participants...
April 7, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28375740/experimentally-dissecting-the-origins-of-peroxiredoxin-catalysis
#5
Kimberly J Nelson, Arden Perkins, Amanda E D Van Swearingen, Steven Hartman, Andrew E Brereton, Derek Parsonage, Freddie R Salsbury, P Andrew Karplus, Leslie B Poole
AIMS: Peroxiredoxins (Prxs) are ubiquitous cysteine-based peroxidases involved in oxidant defense and signal transduction. Despite much study, the precise roles of conserved residues remain poorly defined. In this study, we carried out extensive functional and structural characterization of 10 variants of such residues in a model decameric bacterial Prx. RESULTS: Three active site proximal mutations of Salmonella typhimurium AhpC, T43V, R119A, and E49Q, lowered catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but did not affect reactivity toward their reductant, AhpF...
April 4, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28370033/extremely-low-frequency-electromagnetic-field-exposure-enhances-inflammatory-response-and-inhibits-effect-of-antioxidant-in-raw-264-7-cells
#6
Soo Jeong Kim, Ye Won Jang, Kyung Eun Hyung, Da Kyoung Lee, Kee Hyeob Hyun, Seung Hwarn Jeong, Kyung Hoon Min, Wonku Kang, Ji Hoon Jeong, So-Young Park, Kwang Woo Hwang
In recent years, there has been a dramatic increase in the number and variety of electronic devices that emit electromagnetic waves. Because people live and work in close proximity to these pieces of electrical equipment, there is growing concern surrounding the destruction of homeostasis by electromagnetic field exposure. In the present study, the effects of 60 Hz 0.8 mT extremely low-frequency electromagnetic fields (ELF-EMF) on a macrophage cell line (RAW 264.7) were examined. Under defined ELF-EMF exposure conditions, the production of nitric oxide and pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were increased in RAW 264...
March 29, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/28361248/increased-risk-of-critical-cbf-levels-in-sah-patients-with-actual-cpp-below-calculated-optimal-cpp
#7
Ulf Johnson, Henrik Engquist, Anders Lewén, Tim Howells, Pelle Nilsson, Elisabeth Ronne-Engström, Elham Rostami, Per Enblad
BACKGROUND: Cerebral pressure autoregulation can be quantified with the pressure reactivity index (PRx), based on the correlation between blood pressure and intracranial pressure. Using PRx optimal cerebral perfusion pressure (CPPopt) can be calculated, i.e., the level of CPP where autoregulation functions best. The relation between cerebral blood flow (CBF) and CPPopt has not been examined. The objective was to assess to which extent CPPopt can be calculated in SAH patients and to investigate CPPopt in relation to CBF...
March 30, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28358580/peroxiredoxin-5-decreases-beta-amyloid-mediated-cyclin-dependent-kinase-5-activation-through-regulation-of-ca-2-mediated-calpain-activation
#8
Junghyung Park, Bokyung Kim, Unbin Chae, Dong Gil Lee, Min Kyoung Kam, Sang-Rae Lee, Seunghoon Lee, Hyun-Shik Lee, Jeen-Woo Park, Dong-Seok Lee
AIMS: Aberrant Cdk5 (cyclin-dependent kinase 5) and oxidative stress are crucial components of diverse neurodegenerative disorders, including Alzheimer's disease (AD). We previously reported that a change in peroxiredoxin (Prx) expression is associated with protection from neuronal death. The aim of the current study was to analyze the role of Prx in regulating Cdk5 activation in AD. RESULTS: We found that of the six Prx subtypes, Prx5 was increased the most in cellular (N2a-APPswe cells) model of AD...
March 30, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28338762/redox-and-reactive-oxygen-species-network-in-acclimation-for-salinity-tolerance-in-sugar-beet
#9
M Sazzad Hossain, Abdelaleim Ismail ElSayed, Marten Moore, Karl-Josef Dietz
Fine-tuned and coordinated regulation of transport, metabolism and redox homeostasis allows plants to acclimate to osmotic and ionic stress caused by high salinity. Sugar beet is a highly salt tolerant crop plant and is therefore an interesting model to study sodium chloride (NaCl) acclimation in crops. Sugar beet plants were subjected to a final level of 300 mM NaCl for up to 14 d in hydroponics. Plants acclimated to NaCl stress by maintaining its growth rate and adjusting its cellular redox and reactive oxygen species (ROS) network...
February 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28330412/early-asymmetric-cardio-cerebral-causality-and-outcome-after-severe-traumatic-brain-injury
#10
Lei Gao, Peter Smieleweski, Marek Czosnyka, Ari Ercole
The brain and heart are two vital systems in health and disease, increasingly recognised as a complex, interdependent network with constant information flow in both directions. After severe traumatic brain injury (TBI), the causal, directed interactions between the brain, heart and autonomic nervous system have not been well established. Novel methods are needed to probe unmeasured, potentially prognostic information in complex biological networks that are not revealed via traditional means. In this study, we examined potential bi-directional causality between intracranial pressure (ICP), mean arterial pressure (MAP) and heart rate (HR) and its relationship to mortality in a 24-hour period early after TBI...
March 23, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28314261/peroxiredoxin-expression-of-human-osteosarcoma-cells-is-influenced-by-cold-atmospheric-plasma-treatment
#11
Denis Gümbel, Nadine Gelbrich, Matthias Napp, Georg Daeschlein, Axel Kramer, Axel Sckell, Martin Burchardt, Axel Ekkernkamp, Matthias B Stope
BACKGROUND/AIM: To evaluate the potential involvement of redox-specific signalling pathways in cold atmospheric plasma (CAP)-induced apoptosis on human osteosarcoma cells. MATERIALS AND METHODS: Osteosarcoma cell lines were treated with CAP with or without antioxidative agents and seeded in cell culture plates. Cell proliferation was determined by counting viable cells. Carrier gas-treated cells served as control. Peroxiredoxin (PRX) 1-3 expression and secretion were assessed...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28299982/oligo-ethylene-glycol-modified-%C3%AE-cyclodextrin-based-polyrotaxanes-for-simultaneously-modulating-solubility-and-cellular-internalization-efficiency
#12
Atsushi Tamura, Moe Ohashi, Nobuhiko Yui
We developed stimuli-labile polyrotaxanes (PRXs) composed of β-cyclodextrin (β-CD), Pluronic as an axle polymer, and acid-cleavable N-triphenylmethyl groups as bulky stopper molecules, and found that the PRXs are potent therapeutics for Niemann-Pick type C disease, because the PRX can effectively reduce intracellular cholesterol through the intracellular release of threaded β-CDs. In general, the PRXs need to be chemically modified with hydrophilic functional groups because PRXs are not soluble in aqueous media...
March 16, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28296409/microfibrous-solid-dispersions-of-poorly-water-soluble-drugs-produced-via-centrifugal-spinning-unexpected-dissolution-behavior-on-recrystallization
#13
Stefania Marano, Susan A Barker, Bahijja T Raimi-Abraham, Shahrzad Missaghi, Ali Rajabi-Siahboomi, Abil E Aliev, Duncan Q M Craig
Temperature-controlled, solvent-free centrifugal spinning may be used as a means of rapid production of amorphous solid dispersions in the form of drug-loaded sucrose microfibers. However, due to the high content of amorphous sucrose in the formulations, such microfibers may be highly hygroscopic and unstable on storage. In this study, we explore both the effects of water uptake of the microfibers and the consequences of deliberate recrystallization for the associated dissolution profiles. The stability of sucrose microfibers loaded with three selected BCS class II model drugs (itraconazole (ITZ), olanzapine (OLZ), and piroxicam (PRX)) was investigated under four different relative humidity conditions (11, 33, 53, and 75% RH) at 25 °C for 8 months, particularly focusing on the effect of the highest level of moisture (75% RH) on the morphology, size, drug distribution, physical state, and dissolution performance of microfibers...
March 27, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28293090/proteomic-profiling-of-fetal-esophageal-epithelium-esophageal-cancer-and-tumor-adjacent-esophageal-epithelium-and-immunohistochemical-characterization-of-a-representative-differential-protein-prx6
#14
Jun-Hui Guo, Guo-Lan Xing, Xin-Hui Fang, Hui-Fang Wu, Bo Zhang, Jin-Zhong Yu, Zong-Min Fan, Li-Dong Wang
AIM: To understand the molecular mechanism of esophageal cancer development and provide molecular markers for screening high-risk populations and early diagnosis. METHODS: Two-dimensional electrophoresis combined with mass spectrometry were adopted to screen differentially expressed proteins in nine cases of fetal esophageal epithelium, eight cases of esophageal cancer, and eight cases of tumor-adjacent normal esophageal epithelium collected from fetuses of different gestational age, or esophageal cancer patients from a high-risk area of esophageal cancer in China...
February 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28289905/expression-profile-of-desiccation-tolerance-factors-in-intertidal-seaweed-species-during-the-tidal-cycle
#15
Camila Fierro, Camilo López-Cristoffanini, Andrés Meynard, Carlos Lovazzano, Francisco Castañeda, Eduardo Guajardo, Loretto Contreras-Porcia
The transcriptional modulation of desiccation tolerance factors in P. orbicularis explains its successful recuperation after water deficit. Differential responses to air exposure clarify seaweed distribution along intertidal rocky zones. Desiccation-tolerant seaweed species, such as Pyropia orbicularis, can tolerate near 96% water loss during air exposure. To understand the phenotypic plasticity of P. orbicularis to desiccation, several tolerance factors were assessed by RT-qPCR, Western-blot analysis, and enzymatic assays during the natural desiccation-rehydration cycle...
March 13, 2017: Planta
https://www.readbyqxmd.com/read/28255331/detection-of-impaired-cerebral-autoregulation-using-selected-correlation-analysis-a-validation-study
#16
Martin A Proescholdt, Rupert Faltermeier, Sylvia Bele, Alexander Brawanski
Multimodal brain monitoring has been utilized to optimize treatment of patients with critical neurological diseases. However, the amount of data requires an integrative tool set to unmask pathological events in a timely fashion. Recently we have introduced a mathematical model allowing the simulation of pathophysiological conditions such as reduced intracranial compliance and impaired autoregulation. Utilizing a mathematical tool set called selected correlation analysis (sca), correlation patterns, which indicate impaired autoregulation, can be detected in patient data sets (scp)...
2017: Computational and Mathematical Methods in Medicine
https://www.readbyqxmd.com/read/28245300/anti-inflammatory-and-antioxidative-effects-of-camellia-japonica-on-human-corneal-epithelial-cells-and-experimental-dry-eye-in-vivo-and-in-vitro-study
#17
Hyo Seok Lee, Joo-Hee Choi, Lian Cui, Ying Li, Jee Myung Yang, Je-Jung Yun, Ji Eun Jung, Won Choi, Kyung Chul Yoon
Purpose: To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). Methods: Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganese-dependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28226215/multiple-functions-and-regulation-of-mammalian-peroxiredoxins
#18
Sue Goo Rhee, In Sup Kil
Peroxiredoxins (Prxs) constitute a major family of peroxidases, with mammalian cells expressing six Prx isoforms (PrxI to PrxVI). Cells produce hydrogen peroxide (H2O2) at various intracellular locations where it can serve as a signaling molecule. Given that Prxs are abundant and possess a structure that renders the cysteine (Cys) residue at the active site highly sensitive to oxidation by H2O2, the signaling function of this oxidant requires extensive and highly localized regulation. Recent findings on the reversible regulation of PrxI through phosphorylation at the centrosome and on the hyperoxidation of the Cys at the active site of PrxIII in mitochondria are described in this review as examples of such local regulation of H2O2 signaling...
February 2, 2017: Annual Review of Biochemistry
https://www.readbyqxmd.com/read/28219939/peroxiredoxin-1-prx1-is-a-dual-function-enzyme-by-possessing-cys-independent-catalase-like-activity
#19
Cen-Cen Sun, Wei-Ren Dong, Tong Shao, Jiang-Yuan Li, Jing Zhao, Li Nie, Li-Xin Xiang, Guan Zhu, Jian-Zhong Shao
Peroxiredoxin (Prx) was previously known as a Cys-dependent thioredoxin. However, we unexpected observed that Prx1 from the green spotted puffer fish Tetraodon nigroviridis (TnPrx1) was able to reduce H2O2 in a manner independent on the Cys peroxidation and reductants. This study aimed to validate the novel function for Prx1, delineate the biochemical features and explore its antioxidant role in cells. We have confirmed that Prx1 from the puffer fish and humans truly possesses a catalase-like activity that is independent of Cys residues and reductants, but dependent on iron...
February 20, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28197170/glutathionylation-of-pea-chloroplast-2-cys-prx-and-mitochondrial-prx-iif-affects-their-structure-and-peroxidase-activity-and-sulfiredoxin-deglutathionylates-only-the-2-cys-prx
#20
Aingeru Calderón, Alfonso Lázaro-Payo, Iván Iglesias-Baena, Daymi Camejo, Juan J Lázaro, Francisca Sevilla, Ana Jiménez
Together with thioredoxins (Trxs), plant peroxiredoxins (Prxs), and sulfiredoxins (Srxs) are involved in antioxidant defense and redox signaling, while their regulation by post-translational modifications (PTMs) is increasingly regarded as a key component for the transduction of the bioactivity of reactive oxygen and nitrogen species. Among these PTMs, S-glutathionylation is considered a protective mechanism against overoxidation, it also modulates protein activity and allows signaling. This study explores the glutathionylation of recombinant chloroplastic 2-Cys Prx and mitochondrial Prx IIF from Pisum sativum...
2017: Frontiers in Plant Science
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