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https://www.readbyqxmd.com/read/28454428/the-regulation-of-proteins-associated-with-the-cytoskeleton-by-hepatitis-b-virus-x-protein-during-hepatocarcinogenesis
#1
Fanyun Kong, Hongjuan You, Renxian Tang, Kuiyang Zheng
Hepatocellular carcinoma (HCC) is a major malignant disease worldwide, and chronic hepatitis B virus (HBV) infection is one of the primary causes for this type of cancer. Hepatitis B virus X protein (HBx) is a non-structural protein encoded by the viral genome that has significant effects on the pathogenesis of HCC. With the development of high-throughput assays and technologies, the abnormal HBx-induced expression of certain cellular proteins with assorted biological functions has been investigated. These target proteins identified by various methods include specific proteins associated with the cellular cytoskeleton, which contribute to HBx-induced hepatocarcinogenesis...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454295/nima-related-kinase-2-regulates-hepatocellular-carcinoma-cell-growth-and-proliferation
#2
Xiao-Bo Lai, Yu-Qiang Nie, Hong-Li Huang, Ying-Fei Li, Chuang-Yu Cao, Hui Yang, Bo Shen, Zhi-Qiang Feng
NIMA-related kinase 2 (Nek2) is often upregulated in human cancer and is important in regulating the cell cycle and gene expression, and maintaining centrosomal structure and function. The present study aimed to investigate the expression pattern, clinical significance, and biological function of Nek2 in hepatocellular carcinoma (HCC). mRNA and protein levels of Nek2 were examined in HCC and corresponding normal liver tissues. The MTT and soft agar colony formation assays, and flow cytometry were employed to assess the roles of Nek2 in cell proliferation and growth...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454057/application-of-2d-nmr-with-room-temperature-nmr-probes-for-the-assessment-of-the-higher-order-structure-of-filgrastim
#3
Robert G Brinson, Houman Ghasriani, Derek J Hodgson, Kristie M Adams, Ian McEwen, Darón I Freedberg, Kang Chen, David A Keire, Yves Aubin, John P Marino
The higher order structure (HOS) of biotherapeutics is a critical quality attribute that can be evaluated by nuclear magnetic resonance (NMR) spectroscopy at atomic resolution. NMR spectral mapping of HOS can be used to establish HOS consistency of a biologic across manufacturing changes or to compare a biosimilar to an innovator reference product. A previous inter-laboratory study performed using filgrastim drug products demonstrated that two-dimensional (2D)-NMR (1)H(N)-(15)N(H) heteronuclear correlation spectroscopy is a highly robust and precise method for mapping the HOS of biologic drugs at natural abundance using high sensitivity NMR 'cold probes...
April 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28454046/preliminary-investigation-of-catalytic-antioxidant-anticancer-and-bactericidal-activity-of-green-synthesized-silver-and-gold-nanoparticles-using-actinidia-deliciosa
#4
Saraschandra Naraginti, Yi Li
Herein we report a rapid low cost one step green synthetic method using Actinidia deliciosa fruit extract for preparation of stable and multifunctional silver and gold nanoparticles. The synthesized nanoparticles were successfully used as green catalysts for the reduction of 4-nitrophenol (4-NP) and methylene blue (MB). The enhanced biological activity of the prepared nanoparticles was investigated based on its highly stable antioxidant, anticancer and bactericidal effects. TEM micrographs showed that the silver nanoparticles (AgNPs) formed were predominantly spherical in shape having diameters ranging from 25 to 40nm, while gold nanoparticles (AuNPs) shown particle size ranges from 7 to 20nm...
March 30, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28453995/synthesis-and-mechanistic-evaluation-of-novel-n-benzylidene-carbohydrazide-1h-pyrazolo-3-4-b-pyridine-derivatives-as-non-anionic-antiplatelet-agents
#5
André L Lourenço, Raquel R S Salvador, Leonardo A Silva, Max S Saito, Juliana F R Mello, Lúcio M Cabral, Carlos R Rodrigues, Maria A F Vera, Estela M F Muri, Alessandra M T de Souza, Charles S Craik, Luiza R S Dias, Helena C Castro, Plínio C Sathler
Cardiovascular diseases (CVDs) account for over 17 million deaths globally each year, with atherosclerosis as the underlying cause of most CVDs. Herein we describe the synthesis and in vitro mechanistic evaluation of novel N'-benzylidene-carbohydrazide-1H-pyrazolo[3,4-b]pyridines (3-22) designed as non-anionic antiplatelet agents and presenting a 30-fold increase in potency compared to aspirin. The mechanism underlying their antiplatelet activity was elucidated by eliminating potential targets through a series of in vitro assays including light transmission aggregometry, clot retraction, and quantitative ELISA, further identifying the reduction in biosynthesis of thromboxane B2 as their main mechanism of action...
April 14, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28453994/synthesis-and-applications-of-benzohydroxamic-acid-based-histone-deacetylase-inhibitors
#6
REVIEW
Rob De Vreese, Matthias D'hooghe
This paper provides an overview of the synthesis and biological activity of the most representative benzohydroxamic acid-based histone deacetylase inhibitors published to date. Benzohydroxamic acids comprise an important class of HDAC inhibitors, and recently several of these structures have been evaluated in clinical trials for the treatment of a variety of cancers. In this overview, benzohydroxamic acids were divided in four different classes based on their reported selectivity towards zinc-dependent HDACs: a first and major class consists of HDAC6 selective inhibitors, a second class deals with pan-HDAC inhibitors, a third class comprises HDAC8 selective inhibitors and a fourth, minor class includes dual HDAC6/8 selective inhibitors...
April 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28453861/functional-lung-avoidance-and-response-adaptive-escalation-flare-rt-multimodality-plan-dosimetry-of-a-precision-radiation-oncology-strategy
#7
Eunsin Lee, Jing Zeng, Robert S Miyaoka, Jatinder Saini, Paul E Kinahan, George A Sandison, Tony Wong, Hubert J Vesselle, Ramesh Rengan, Stephen R Bowen
PURPOSE: Non-small cell lung cancer (NSCLC) patient radiation therapy (RT) is planned without consideration of spatial heterogeneity in lung function or tumor response. We assessed the dosimetric and clinical feasibility of functional lung avoidance and response-adaptive escalation (FLARE) RT to reduce dose to [(99m) Tc]MAA-SPECT/CT perfused lung while redistributing an escalated boost dose within [(18) F]FDG-PET/CT-defined biological target volumes (BTV). METHODS: Eight stage IIB-IIIB NSCLC patients underwent FDG-PET/CT and MAA-SPECT/CT treatment planning scans...
April 28, 2017: Medical Physics
https://www.readbyqxmd.com/read/28453814/graphene-specimen-support-technique-for-low-voltage-stem-imaging
#8
Masao Yamashita, Matthew Ryan Leyden, Hidehito Adaniya, Martin Philip Cheung, Teruhisa Hirai, Yabing Qi, Tsumoru Shintake
The scanning transmission electron microscopy (STEM) mode of today's field emission scanning electron microscopes enables sub-nanometer resolution imaging. Graphene is a single-atom thick, electrically conductive material, making it an excellent specimen support for the low voltage STEM imaging of nanometer-sized objects such as viruses. Here we present low voltage STEM images of bacteriophage T4 recorded on highly cleaned graphene films. The results show that ultrathin graphene support films markedly improve image signal at low accelerating voltages...
April 27, 2017: Microscopy
https://www.readbyqxmd.com/read/28453668/a-mass-graph-based-approach-for-the-identification-of-modified-proteoforms-using-top-down-tandem-mass-spectra
#9
Qiang Kou, Si Wu, Nikola Tolic, Ljiljana Paša-Tolic, Yunlong Liu, Xiaowen Liu
Motivation: Although proteomics has rapidly developed in the past decade, researchers are still in the early stage of exploring the world of complex proteoforms, which are protein products with various primary structure alterations resulting from gene mutations, alternative splicing, post-translational modifications, and other biological processes. Proteoform identification is essential to mapping proteoforms to their biological functions as well as discovering novel proteoforms and new protein functions...
May 1, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28453644/traitrateprop-a-web-server-for-the-detection-of-trait-dependent-evolutionary-rate-shifts-in-sequence-sites
#10
Eli Levy Karin, Haim Ashkenazy, Susann Wicke, Tal Pupko, Itay Mayrose
Understanding species adaptation at the molecular level has been a central goal of evolutionary biology and genomics research. This important task becomes increasingly relevant with the constant rise in both genotypic and phenotypic data availabilities. The TraitRateProp web server offers a unique perspective into this task by allowing the detection of associations between sequence evolution rate and whole-organism phenotypes. By analyzing sequences and phenotypes of extant species in the context of their phylogeny, it identifies sequence sites in a gene/protein whose evolutionary rate is associated with shifts in the phenotype...
April 27, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28453488/assessment-of-novel-curcumin-derivatives-as-potent-inhibitors-of-inflammation-and-amyloid-%C3%AE-aggregation-in-alzheimer-s-disease
#11
Johant Lakey-Beitia, Yisett González, Deborah Doens, David E Stephens, Ricardo Santamaría, Enrique Murillo, Marcelino Gutiérrez, Patricia L Fernández, K S Rao, Oleg V Larionov, Armando A Durant-Archibold
Alzheimer's disease (AD) is the most common neurodegenerative disorder affecting the elderly population worldwide. Brain inflammation plays a key role in the progression of AD. Deposition of senile plaques in the brain stimulates an inflammatory response with the overexpression of pro-inflammatory mediators, such as the neuroinflammatory cytokine. interleukin-6. Curcumin has been revealed to be a potential agent for treating AD following different neuroprotective mechanisms, such as inhibition of aggregation and decrease in brain inflammation...
April 25, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28449207/targeting-anti-apoptotic-bcl2-family-proteins-in-haematological-malignancies-from-pathogenesis-to-treatment
#12
REVIEW
Meike Vogler, Harriet S Walter, Martin J S Dyer
The B-cell lymphoma 2 (BCL2) family of proteins comprise key regulators of apoptosis and are implicated in the pathogenesis of many malignancies, including lymphomas and leukaemias. Targeting of BCL2 proteins can be directly toxic to tumour cells or render them more sensitive to chemotherapy. Inhibition of the anti-apoptotic functions of BCL2 proteins using structure-based design to produce specific inhibitors of protein-protein interactions has been achieved for BCL2, MCL1 and BCL-XL (also termed BCL2L1), providing an armamentarium of new targeted therapies called BH3-mimetics...
April 27, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28449114/neuro-symbolic-representation-learning-on-biological-knowledge-graphs
#13
Mona Alshahrani, Mohammed Asif Khan, Omar Maddouri, Akira R Kinjo, Núria Queralt-Rosinach, Robert Hoehndorf
Motivation: Biological data and knowledge bases increasingly rely on Semantic Web technologies and the use of knowledge graphs for data integration, retrieval and federated queries. In the past years, feature learning methods that are applicable to graph-structured data are becoming available, but have not yet widely been applied and evaluated on structured biological knowledge. Results: We develop a novel method for feature learning on biological knowledge graphs...
April 25, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28449087/methyl-cpg-binding-domain-protein-1-regulates-localization-and-activity-of-tet1-in-a-cxxc3-domain-dependent-manner
#14
Peng Zhang, Cathia Rausch, Florian D Hastert, Boyana Boneva, Alina Filatova, Sujit J Patil, Ulrike A Nuber, Yu Gao, Xinyu Zhao, M Cristina Cardoso
Cytosine modifications diversify and structure the genome thereby controlling proper development and differentiation. Here, we focus on the interplay of the 5-methylcytosine reader Mbd1 and modifier Tet1 by analyzing their dynamic subcellular localization and the formation of the Tet oxidation product 5-hydroxymethylcytosine in mammalian cells. Our results demonstrate that Mbd1 enhances Tet1-mediated 5-methylcytosine oxidation. We show that this is due to enhancing the localization of Tet1, but not of Tet2 and Tet3 at heterochromatic DNA...
April 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28449072/functions-of-galectins-as-self-non-self-recognition-and-effector-factors
#15
Gerardo R Vasta, Chiguang Feng, Nuria González-Montalbán, Justin Mancini, Lishi Yang, Kelsey Abernathy, Graeme Frost, Cheyenne Palm
Carbohydrate structures on the cell surface encode complex information that through specific recognition by carbohydrate-binding proteins (lectins), modulates interactions between cells, cells and the extracellular matrix, or mediates recognition of potential microbial pathogens. Galectins are a family of ß-galactoside-binding lectins, which are evolutionary conserved and have been identified in most organisms, from fungi to invertebrates and vertebrates, including mammals. Since their discovery in the 1970s, their biological roles, initially understood as limited to recognition of endogenous carbohydrate ligands in embryogenesis and development, have expanded in recent years by the discovery of their roles in tissue repair and regulation of immune homeostasis...
April 25, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28449031/rnablueprint-flexible-multiple-target-nucleic-acid-sequence-design
#16
Stefan Hammer, Birgit Tschiatschek, Christoph Flamm, Ivo L Hofacker, Sven Findeiß
Motivation: Realizing the value of synthetic biology in biotechnology and medicine requires the design of molecules with specialized functions. Due to its close structure to function relationship, and the availability of good structure prediction methods and energy models, RNA is perfectly suited to be synthetically engineered with predefined properties. However, currently available RNA design tools cannot be easily adapted to accommodate new design specifications. Furthermore, complicated sampling and optimization methods are often developed to suit a specific RNA design goal, adding to their inflexibility...
April 25, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28448728/does-an-injection-of-adipose-derived-mesenchymal-stem-cells-loaded-in-fibrin-glue-influence-rotator-cuff-repair-outcomes-a-clinical-and-magnetic-resonance-imaging-study
#17
Yong Sang Kim, Chang Hun Sung, Sung Hoon Chung, Sang Joon Kwak, Yong Gon Koh
BACKGROUND: The mesenchymal stem cell (MSC)-based tissue engineering approach has been developed to improve the treatment of rotator cuff tears. Hypothesis/Purpose: The purpose was to determine the effect of an injection of adipose-derived MSCs loaded in fibrin glue during arthroscopic rotator cuff repair on clinical outcomes and to evaluate its effect on structural integrity using magnetic resonance imaging (MRI). The hypothesis was that the application of adipose-derived MSCs would improve outcomes after the surgical repair of a rotator cuff tear...
April 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28448436/role-of-archaeal-hera-protein-in-the-biology-of-the-bacterium-thermus-thermophilus
#18
Alba Blesa, Nieves G Quintans, Ignacio Baquedano, Carlos P Mata, José R Castón, José Berenguer
Intense gene flux between prokaryotes result in high percentage of archaeal genes in the genome of the thermophilic bacteria Thermus spp. Among these archaeal genes a homolog to the Sulfolobus spp. HerA protein appears in all of the Thermus spp. strains so far sequenced (HepA). The role of HepA in Thermus thermophilus HB27 has been analyzed using deletion mutants, and its structure resolved at low resolution by electron microscopy. Recombinant HepA shows DNA-dependent ATPase activity and its structure revealed a double ring, conically-shaped hexamer with an upper diameter of 150 Å and a bottom module of 95 Å...
April 27, 2017: Genes
https://www.readbyqxmd.com/read/28448135/total-synthesis-of-%C3%A2-lycojaponicumin-d-and-lycodoline-type-lycopodium-alkaloids
#19
Xian-He Zhao, Qing Zhang, Ji-Yuan Du, Xin-Yun Lu, Ye-Xing Cao, Yu-Hua Deng, Chun-An Fan
Lycopodium alkaloids with structural diversity and biological significance have been stimulating an increasing interest in synthetic and medicinal communities, in which inspiration and exploration of their related biogenetic relationship generally comprises one of the major concerns. Driven by the plausible biogenetic entry to lycojaponicumin D as the first member of Lycopodium alkaloids having structurally unusual C(3)-C(13) linked scaffold, a new connection with lycodoline has been proposed and discovered on the basis of the design of an unprecedented bioinspired tandem fragmentation/Mannich reaction...
April 27, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28448052/an-in-vitro-organ-culture-model-of-the-murine-intervertebral-disc
#20
Jennifer W Liu, Kevin H Lin, Christian Weber, Sameer Bhalla, Sean Kelso, Kaixi Wang, Simon Y Tang
Intervertebral disc (IVD) degeneration is a significant contributor to low back pain. The IVD is a fibrocartilaginous joint that serves to transmit and dampen loads in the spine. The IVD consists of a proteoglycan-rich nucleus pulposus (NP) and a collagen-rich annulus fibrosis (AF) sandwiched by cartilaginous end-plates. Together with the adjacent vertebrae, the vertebrae-IVD structure forms a functional spine unit (FSU). These microstructures contain unique cell types as well as unique extracellular matrices...
April 11, 2017: Journal of Visualized Experiments: JoVE
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