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https://www.readbyqxmd.com/read/29775897/the-gastric-mucosal-protective-effects-of-astragaloside-iv-in-mnng-induced-gpl-rats
#1
Tiantian Cai, Chengzhe Zhang, Ziming Zhao, Siyi Li, Haobin Cai, Xiaodong Chen, Dake Cai, Wei Liu, Yan Yan, Kaifeng Xie, Huafeng Pan, Xiaohui Zeng
Gastric Cancer is one of the most common types of cancer. And the occurrence of gastric carcinoma is an evolutionary histopathological stage. As a result, further research of GPL, which is a borderline of gastric cancer, is indispensable for preventing the formation and development of gastric carcinoma. Several studies have demonstrated a correlation between the expression of autophagy, apoptosis and Gastric cancer (GC). However, the effects of autophagy and apoptosis on human gastric cancer progression, particularly on gastric precancerous lesions (GPL), have not totally been investigated...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29775819/facile-synthesis-of-yolk-shell-structured-monodisperse-mesoporous-organosilica-nanoparticles-by-a-mild-alkalescent-etching-approach
#2
Jun Tao, Meng Dang, Xiaodan Su, Qing Hao, Junjie Zhang, Xiaobo Ma, Guangming Lu, Yunlei Zhang, Ying Tian, Lixing Weng, Zhaogang Teng, Lianhui Wang
In the work, yolk-shell structured mesoporous organosilica nanoparticles (YSMONs) are successfully prepared by a mild alkalescent etching approach. The method is very convenient, in which mesostructured organosilica nanospheres are directly transformed into yolk-shell structures after etching with mild alkalescent solution (e.g. sodium carbonate solution). The prepared YSMONs have ethane-bridged frameworks, a monodisperse diameter (320 nm), a large pore volume (1.0 cm3  g-1 ), a uniform mesopore (2.4 nm) and a high surface area (1327 m2  g-1 )...
May 15, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29775775/knowledge-and-learning-of-verb-biases-in-amnesia
#3
Rachel Ryskin, Zhenghan Qi, Natalie V Covington, Melissa Duff, Sarah Brown-Schmidt
Verb bias-the co-occurrence frequencies between a verb and the syntactic structures it may appear with-is a critical and reliable linguistic cue for online sentence processing. In particular, listeners use this information to disambiguate sentences with multiple potential syntactic parses (e.g., Feel the frog with the feather.). Further, listeners dynamically update their representations of specific verbs in the face of new evidence about verb-structure co-occurrence. Yet, little is known about the biological memory systems that support the use and dynamic updating of verb bias...
May 15, 2018: Brain and Language
https://www.readbyqxmd.com/read/29775713/enhancement-of-catalytic-activity-and-thermostability-of-a-thermostable-cellobiohydrolase-from-chaetomium-thermophilum-by-site-directed-mutagenesis
#4
Chao Han, Weiguang Li, Chengyao Hua, Fengqing Sun, Pengsheng Bi, Qunqing Wang
Enzymatic saccharification of lignocellulosic biomass is increasingly applied in agricultural and industrial applications. Nevertheless, low performance in the extreme environment severely prevents the utilization of commercial enzyme preparations. To obtain cellobiohydrolases with improved catalytic activity and thermostability, structure-based rational design was performed based on a thermostable cellobiohydrolase CtCel6 from Chaetomium thermophilum. In the present study, four conserved and noncatalytic residue substitutions were generated via site-directed mutagenesis...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775664/auto-induction-for-high-level-production-of-biologically-active-reteplase-in-escherichia-coli
#5
Mehrnoosh Fathi-Roudsari, Nader Maghsoudi, Amirhossein Maghsoudi, Sepideh Niazi, Morvarid Soleiman
Reteplase is a third generation tissue plasminogen activator (tPA) with a modified structure and prolonged half-life in comparison to native tPA. As a non-glycosylated protein, reteplase is expressed in Escherichia coli. Due to presence of several disulfide bonds, high level production of reteplase is complicated and needs extra steps for conversion to biologically active form. Auto-induction represents a method for high-yield growth of bacterial cells and higher expression of recombinant proteins. Here we have tried to optimize the auto-induction procedure for soluble and active expression of reteplase in E...
May 15, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29775648/towards-a-quantitative-model-to-predict-the-toxicity-pathogenicity-potential-of-mineral-fibers
#6
Alessandro F Gualtieri
A number of mineral fibers represent a health hazard because they are classified as cancer-causing chemical/physical toxicants upon (chronic) dust inhalation. Although in the last decades they have been the subject of intensive multidisciplinary investigations, the mechanisms by which mineral fibers induce toxic and pathogenic adverse effects on human health and environment are not yet fully understood. The major intricacy of the biological approach that prevents the design of a conclusive shared model of behavior of mineral fibers in a biological system stems from their very nature with intrinsic variability in chemical, molecular, structural and morphometric parameters, biodurability and surface reactivity...
May 15, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29775569/modification-of-chickpea-cystatin-by-reactive-dicarbonyl-species-glycation-oxidation-and-aggregation
#7
Sheraz Ahmad Bhat, Waseem Feeroze Bhat, Mohammad Afsar, Mohd Shahnawaz Khan, Bilqees Bano
Reactive dicarbonyl species such as methylglyoxal (MGO) and glyoxal (GO) have recently received extensive attention due to their high reactivity and ability to modify biological substances such as proteins, phospholipids, and DNA. In case of proteins these reactive species mainly react with lysine and arginine residues to form AGEs, oxidative products, and aggregates. Chickpea cystatin (CPC) was incubated with varying concentrations of glyoxal and methylglyoxal which caused, along with altered secondary and tertiary structures, glycation, functional inactivation, altered redox state, cross-linking and high-molecular-mass aggregation...
May 15, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29775224/circular-rnas-are-differentially-expressed-in-liver-ischemia-reperfusion-injury-model
#8
Zhiqiang Ye, Qinglei Kong, Jianhua Han, Jingyi Deng, Miaolue Wu, Hong Deng
Liver ischemia/reperfusion (I/R) injury has high mortality due to the intense inflammatory process occurs in the liver. However, the pathological mechanism underlying I/R injury is still not clear. Recent works showed that circular RNAs play critical roles in many human diseases. In this study, the occurrence of liver I/R injury was validated by an analysis of the blood samples and hematoxylin-eosin (HE) staining of liver tissues. Total RNA was purified and followed by RNA-seq in the purpose of screening the circRNAs in significant differentially expression, which were validated by quantitative PCR...
May 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29775069/simultaneous-release-and-labelling-of-o-and-n-glycans-allowing-for-rapid-glycomic-analysis-by-online-lc-uv-esi-ms-ms
#9
Chengjian Wang, Yu Lu, Jianli Han, Wanjun Jin, Lingmei Li, Ying Zhang, Xuezheng Song, Linjuan Huang, Zhongfu Wang
Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming. Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed. Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labelled in situ and thus available for further characterization. In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by beta-elimination and N-glycans liberated by alkaline hydrolysis...
May 18, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29775068/embedding-a-metal-binding-motif-for-copper-transporter-into-a-lipid-bilayer-by-cu-i-binding
#10
Mariko Okada, Shinji Kajimoto, Takakazu Nakabayashi
Peptide-lipid interactions are widely involved with biologically significant phenomena, including the pathogenic mechanisms of protein misfolding diseases, and transmembrane protein folding. In this paper, the interaction of the cysteine/tryptophan (Cys/Trp) motif, which is a metal binding motif of copper transporter (Ctr) proteins, with a lipid bilayer was studied using fluorescence and circular dichroism (CD) spectroscopy. The binding of Cu(I) to the Cys/Trp motif induced a large red-edge excitation shift (REES) in the Trp fluorescence, indicating that the Trp residue is located inside the lipid bilayer following complexation of Cu(I) with the Cys/Trp motif...
May 18, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29775059/intrinsic-antioxidant-potential-of-the-aminoindole-structure-a-computational-kinetics-study-of-tryptamine
#11
Erika N Bentz, Rosana Maria Lobayan, Henar Martinez, Pilar Redondo, Antonio Largo
A computational kinetics study of the antioxidant activity of tryptamine toward HO● and HOO● radicals in water at 298 K has been carried out. Density functional methods have been employed for the quantum chemical calculations and conventional transition state theory was used for rate constants evaluation. Different mechanisms have been considered: radical adduct formation (RAF), single electron transfer (SET), and hydrogen atom transfer (HAT). For the reaction of tryptamine with the hydroxyl radical nearly all channels are diffusion controlled, and the overall rate constant is very high, 6...
May 18, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29774971/3d-dna-origami-crystals
#12
Tao Zhang, Caroline Hartl, Kilian Frank, Amelie Heuer-Jungemann, Stefan Fischer, Philipp C Nickels, Bert Nickel, Tim Liedl
3D crystals assembled entirely from DNA provide a route to design materials on a molecular level and to arrange guest particles in predefined lattices. This requires design schemes that provide high rigidity and sufficiently large open guest space. A DNA-origami-based "tensegrity triangle" structure that assembles into a 3D rhombohedral crystalline lattice with an open structure in which 90% of the volume is empty space is presented here. Site-specific placement of gold nanoparticles within the lattice demonstrates that these crystals are spacious enough to efficiently host 20 nm particles in a cavity size of 1...
May 18, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29774900/multiplex-detection-of-quality-indicator-molecule-targets-in-urine-using-programmable-hairpin-probes-based-on-a-simple-double-t-type-microchip-electrophoresis-platform-and-isothermal-polymerase-catalyzed-target-recycling
#13
Lingying Zhou, Ning Gan, Yongxiang Wu, Futao Hu, Jianyuan Lin, Yuting Cao, Dazhen Wu
Recently, it has been crucial to be able to detect and quantify small molecular targets simultaneously in biological samples. Herein, a simple and conventional double-T type microchip electrophoresis (MCE) based platform for the multiplex detection of quality indicator molecule targets in urine, using ampicillin (AMPI), adenosine triphosphate (ATP) and estradiol (E2) as models, was developed. Several programmable hairpin probes (PHPs) were designed for detecting different targets and triggering isothermal polymerase-catalyzed target recycling (IPCTR) for signal amplification...
May 18, 2018: Analyst
https://www.readbyqxmd.com/read/29774883/-properties-of-decellularized-rabbit-carotid-artery-with-two-different-cross-linked-technologies-a-comparative-study
#14
Lei Huang, Feng Kuang, Demiao Kong, Xinmin Zhou
To compare the properties between decellularized rabbit carotid artery with different cross-linked technologies.
 Methods: The decellularized rabbit carotid arteries were randomly divided into a photo-oxidation group and a procyanidins group. One group was cross-linked with photo-oxidation and the other group was cross-linked with procyanidins. The in vitro or in vivo properties of the two groups were evaluated by testing heat-shrinking temperature, max tensile strength and the max elongation or by testing tissue structure, inflammatory reaction and calcification degree...
April 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/29774634/study-of-hyperpigmentation-in-human-skin-disorder-using-different-electron-microscopy-techniques
#15
Seulgi Noh, Hyosun Choi, Ji Soo Kim, Il-Hwan Kim, Ji Young Mun
There is a global trend of increase in the demand for three-dimensional electron microscopy with high resolution. The ultrastructural change and related functional studies are necessary to investigate biological phenomena. In this study, currently available 3D reconstruction techniques of electron microscopes (serial block-face scanning electron microscopy and focused ion beam-scanning electron microscopy) were used to investigate hyperpigmentary disorders in human skin. In the basal layer of the epidermis in the human skin, there are melanocytes that produce melanin and keratinocytes that act as a barrier against environmental damage...
May 18, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29774535/physiology-and-molecular-biology-of-barrier-mechanisms-in-the-fetal-and-neonatal-brain
#16
Norman R Saunders, Katarzyna M Dziegielewska, Kjeld Møllgård, Mark D Habgood
Properties of the local internal environment of the adult brain are tightly controlled providing a stable milieu essential for its normal function. The mechanisms involved in this complex control are structural, molecular and physiological (influx and efflux transporters) frequently referred to as the "blood-brain barrier". These mechanisms include regulation of ion levels in brain interstitial fluid essential for normal neuronal function, supply of nutrients, removal of metabolic products and prevention of entry or elimination of toxic agents...
May 17, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29774506/therapeutic-potential-of-mesenchymal-stem-cell-derived-exosomes-in-the-treatment-of-eye-diseases
#17
C Randall Harrell, Bojana Simovic Markovic, Crissy Fellabaum, Aleksandar Arsenijevic, Valentin Djonov, Nebojsa Arsenijevic, Vladislav Volarevic
Mesenchymal stem cells (MSCs) were, due to their immunomodulatory and pro-angiogenic characteristics, extensively explored as new therapeutic agents in cell-based therapy of uveitis, glaucoma, retinal and ocular surface diseases.Since it was recently revealed that exosomes play an important role in biological functions of MSCs, herewith we summarized current knowledge about the morphology, structure, phenotype and functional characteristics of MSC-derived exosomes emphasizing their therapeutic potential in the treatment of eye diseases...
May 18, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29774428/inter-individual-differences-in-serotonin-and-glutamate-co-transmission-reflect-differentiation-in-context-induced-conditioned-50-khz-usvs-response-after-morphine-withdrawal
#18
Adam Hamed, Miron Bartosz Kursa
A growing body of research provides compelling evidence that in rats 50-kHz USVs are a form of expression of positive emotions. Context-induced 50-kHz USVs emission is variable among rats, indicating individual differences in contextual response bound up with pharmacological reward. The aims of this study were to: extract the most important neurotransmitters related to context-induced conditioned 50-kHz USVs response; find biological basis of existing inter-individual differences in context-induced conditioned 50-kHz USVs response; create a model of all-to-all neurotransmitters correlations...
May 17, 2018: Brain Structure & Function
https://www.readbyqxmd.com/read/29774118/proteomic-profiling-of-skeletal-and-cardiac-muscle-in-cancer-cachexia-alterations-in-sarcomeric-and-mitochondrial-protein-expression
#19
Angie M Y Shum, Anne Poljak, Nicholas L Bentley, Nigel Turner, Timothy C Tan, Patsie Polly
Background: Cancer cachexia is observed in more than 50% of advanced cancer patients, and impairs quality of life and prognosis. A variety of pathways are likely to be dysregulated. Hence, a broad-spectrum understanding of the disease process is best achieved by a discovery based approach such as proteomics. Results: More than 300 proteins were identified with > 95% confidence in correct sequence identification, of which 5-10% were significantly differentially expressed in cachectic tissues ( p -value of 0...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29774097/natriuretic-peptides-system-in-the-pulmonary-tissue-of-rats-with-heart-failure-potential-involvement-in-lung-edema-and-inflammation
#20
Emad E Khoury, Safa Kinaneh, Doron Aronson, Offer Amir, Diab Ghanim, Natalia Volinsky, Zaher Azzam, Zaid Abassi
Congestive heart failure (CHF) often leads to progressive cardiac hypertrophy and salt/water retention as evident by peripheral and lung edema. Although the pathogenesis of CHF remains largely unclarified, it is widely accepted that neurohormonal changes and inflammatory processes are profoundly involved in structural and functional deterioration of vital organs including, heart, kidney and lungs. Corin, a cardiac serine protease, is responsible for converting pro-ANP and pro-BNP to biologically active natriuretic peptides (NPs)...
April 24, 2018: Oncotarget
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