Read by QxMD icon Read


Thomas E Rams, Jacqueline D Sautter, Chinhua Y Hsiao, Arie J van Winkelhoff
The accuracy of a phenotypic scheme to recognize periodontal Prevotella intermedia/nigrescens group clinical isolates on primary isolation culture plates was assessed with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 84 fresh subgingival isolates from 23 chronic periodontitis patients were presumptively recognized on anaerobically-incubated enriched Brucella blood agar primary isolation plates as P. intermedia/nigrescens based on their dark-pigmented colony morphology, brick-red autofluorescence under long-wave ultraviolet light, and a negative fluorescence test for lactose production...
June 15, 2018: Anaerobe
Karen Mys, Filip Stockmans, Evie Vereecke, G Harry van Lenthe
Due to the rising life expectancy, bone diseases (e.g. osteoporosis, osteoarthritis) and trauma (e.g. fracture) have become an important socio-economic burden. Accurate visualization and quantification of the bone microstructure in vivo is seen as an important step to enhance diagnosis and treatment. Micro-computed tomography (microCT) has become the gold standard in three-dimensional (3D) imaging of trabecular bone structure. Yet, usage is limited to ex vivo analyses, hence, it cannot be used to evaluate bone and bone adaptive responses in a patient...
June 14, 2018: Bone
Michalina Gramatyka, Agnieszka Skorupa, Maria Sokół
Several lines of evidence indicate that exposure of heart to ionizing radiation increases the risk of cardiotoxicity manifested by heart dysfunction and cardiovascular diseases. It was initially believed that the heart is an organ relatively resistant to radiation. Currently, however, it is suspected that even low doses of radiation (< 2 Gy) may have a negative impact on the cardiovascular system. Cardiotoxicity of ionizing radiation is associated with metabolic changes observed in cardiac cells injured by radiation...
June 15, 2018: Acta Biochimica Polonica
Hiroyuki Kono
This article contains two-dimensional (2D) NMR experimental data for a series of methylcellulose (MC) with different substitution degrees (DS), obtained by the Bruker BioSpin 500 MHz NMR spectrometer (Germany). The data facilitated the 1 H and 13 C chemical shifts of eight anhydroglucose units (AGUs) comprising MC chains-unsubstituted, 2-mono-, 3-mono-, 6-mono-, 2,3-di-, 2,6-di-, 3,6-di-, and 2,3,6-tri-substituted AGUs. Data include analyzed the 2D NMR spectra of the MC samples, which are related to the subject of an article in Carbohydrate Polymers , entitled "NMR characterization of methylcellulose: Chemical shift assignment and mole fraction of monomers in the polymer chains" (Kon et al...
June 2018: Data in Brief
Laurent Dortet, Didier Tandé, Dominique de Briel, Sandrine Bernabeu, Camille Lasserre, Guillaume Gregorowicz, Agnès B Jousset, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated three MALDI-TOF-based techniques to detect carbapenemase-producing Enterobacteriaceae (CPE) from cultured colonies. Methods: The performance of three MALDI-TOF-based techniques, including the commercialized MBT STAR®-Carba IVD Kit (Bruker Daltonics) and two in-house protocols performed on the Microflex LT Biotyper (Bruker Daltonics) and the VITEK® MS Plus (bioMérieux), were compared with those of the RAPIDEC® CARBA NP (bioMérieux)...
June 11, 2018: Journal of Antimicrobial Chemotherapy
Markus Stein, Vanessa Tran, Kimberly A Nichol, Philippe Lagacé-Wiens, Peter Pieroni, Heather J Adam, Christine Turenne, Andrew J Walkty, Anne-Cécile Normand, Marijke Hendrickx, Renaud Piarroux, James A Karlowsky
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is commonly used by clinical microbiology laboratories to identify bacterial pathogens and yeasts, but not for the identification of moulds. Recent progress in extraction protocols and the composition of comparative libraries support potential application of MALDI-TOF MS for mould identification in clinical microbiology laboratories. We evaluated the performance of the Bruker Microflex™ MALDI-TOF MS instrument (Billerica, MA, USA) to identify clinical isolates and reference strains of moulds using three libraries, the Bruker mould library, the National Institutes of Health (NIH) library, and the Mass Spectrometry Identification (MSI) online library, and compared those results to conventional (morphological) and molecular (18S/ITS; gold standard) identification methods...
June 12, 2018: Mycoses
Ângela Graciela Deliga Schröder, Fernando Henrique Westphalen, Julio Cesar Schröder, Ângela Fernandes, Vânia Portela Ditzel Westphalen
INTRODUCTION: The aim of this study was to compare the sensitivity and specificity of digital periapical radiography and cone-beam computed tomographic (CBCT) imaging in the detection of natural and simulated external root resorptions (ERRs) with micro-computed tomographic (micro-CT) imaging as the reference standard. METHODS: One hundred twenty-six teeth were scanned using the SkyScan 1172 micro-CT scanner (Bruker microCT, Kontich, Belgium), and the images were evaluated using NRecon software (Bruker microCT)...
June 5, 2018: Journal of Endodontics
Juan A Sanchis-Gimeno, Susanna Llido, David Guede, Shahed Nalla, Francisco Martinez-Soriano, Esther Blanco-Perez, Jose R Caeiro
BACKGROUND: To date, no information about the cortical bone microstructural properties in atlas vertebrae with arcuate foramen has been reported. As a result, we aimed to test in an experimental model if there is a cortical bone thickening in an atlas vertebra which has an arcuate foramen that may play a protective role against bone fracture. METHODS: We analyzed by means of micro-computed tomography the cortical bone thickness, the cortical volume, and the medullary volume (SkyScan 1172 Bruker micro-CT NV, Kontich, Belgium) in cadaveric dry atlas vertebrae with arcuate foramen and without arcuate foramen...
June 5, 2018: World Neurosurgery
T Plecko, K Berbalk, E Wieland, M Shipkova
BACKGROUND: Oral fluid (OF) is increasingly used as an alternative sample matrix in drug of abuse (DOA) screening. Screening is commonly performed by immunoassays and results confirmed using laborious GC-MS based methods. Therefore, an easy to operate ion trap mass spectrometric (IT-MS) commercial screening method (Toxtyper, Bruker Daltronik, Bremen, Germany) combined with a laboratory-developed sample preparation procedure have been evaluated for their application to OF. METHODS: Oral fluid samples were subjected to protein precipitation followed by HybridSPE-phospholipid extraction...
June 6, 2018: Therapeutic Drug Monitoring
Mi Li, Yuanyuan Tang, Nana Ren, Zuotai Zhang, Yiming Cao
This dataset is the supplementary data for the summited research article Li et al., 2017 [1] and provides detailed data profiles to support the explication about mineral constituents' effect on temperature-dependent structural characterization of carbon fractions in sewage sludge-derived biochar. The elemental compositions of major inorganics in the sewage sludge were detected by X-ray fluorescence spectrometry (XRF, S2-Ranger, Bruker).The images from scanning electron microscope (SEM) were compared between unwashed and acid-washed samples, and revealed the effect of acid washing on the surface morphology and porosity of sewage sludge and the biochar...
April 2018: Data in Brief
Gonca Erkose Genc, Melda Demir, Görkem Yaman, Begum Kayar, Fatih Koksal, Dilek Satana
Nowadays, there is a rising worldwide incidence of diseases caused by nontuberculous mycobacteria (NTM) species, especially in immunocompromised patients and those with underlying chronic pulmonary diseases. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) became a method of choice for the identification of NTM species. The aim of this study was to evaluate MALDI-TOF MS for the identification of NTM isolates compared to the PCR-restriction enzyme analysis (PRA)-hsp65 method...
June 6, 2018: New Microbiologica
José Javier Costa-Alcalde, Gema Barbeito-Castiñeiras, José María González-Alba, Antonio Aguilera, Juan Carlos Galán, María Luisa Pérez-Del-Molino
INTRODUCTION: The American Thoracic Society and the Infectious Diseases Society of America recommend that clinically significant non-tuberculous mycobacteria (NTM) should be identified to the species level in order to determine their clinical significance. The aim of this study was to evaluate identification of rapidly growing NTM (RGM) isolated from clinical samples by using MALDI-TOF MS and a commercial molecular system. The results were compared with identification using a reference method...
June 2, 2018: Enfermedades Infecciosas y Microbiología Clínica
Felix Streckenbach, Ronja Klose, Sönke Langner, Inga Langner, Marcus Frank, Andreas Wree, Anne-Marie Neumann, Änne Glass, Thomas Stahnke, Rudolf F Guthoff, Oliver Stachs, Tobias Lindner
PURPOSE: Ultrahigh-field MRI (UHF-MRI) with an in-plane spatial resolution of less than 100 μm is known as MR microscopy (MRM). MRM provides highly resolved anatomical images and allows quantitative assessment of different tissue types using diffusion-weighted imaging (DWI). The aim of the present study was to evaluate the feasibility of combined in vivo anatomical and quantitative assessment of the developing chicken eye in ovo. PROCEDURES: Thirty-eight fertilized chicken eggs were examined at 7...
May 23, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Pia Baumgart, Holger Kirsten, Rainer Haak, Constanze Olms
BACKGROUND: Implant and superstructure provide a complex system, which has to withstand oral conditions. Concerning the brittleness of many ceramics, fractures are a greatly feared issue. Therefore, polymer-infiltrated ceramic networks (PICNs) were developed. Because of its low Young's modulus and high elastic modulus, the PICN crown on a one-piece zirconia implant might absorb forces to prevent the system from fracturing in order to sustain oral forces. Recommendations for the material of superstructure on zirconia implants are lacking, and only one study investigates PICN crowns on these types of implants...
May 23, 2018: International Journal of Implant Dentistry
Damiana Vulturar, Alexandru Fărcăşanu, Flaviu Turcu, Dan Boitor, Carmen Crivii
Background and aims: The cerebellum ("little brain"), the largest part of hind brain, lies in the posterior cranial fossa, beneath the occipital lobe and dorsal to the brainstem. It develops over a long period: it is one of the first structures in the brain to begin to differentiate, but one of the last to mature. The use of ultrasonography has significantly improved the evaluation of fetal growth and development and has permitted prenatal diagnosis of a variety of congenital malformations...
2018: Clujul Medical (1957)
Keith C da Cunha, Arnaud Riat, Anne-Cecile Normand, Philipp P Bosshard, Margarete T G de Almeida, Renaud Piarroux, Jacques Schrenzel, Lionel Fontao
BACKGROUND: Dermatophytes cause human infections limited to keratinized tissues. We showed that the direct transfer method allows reliable identification of non-dermatophytes mould and yeast by MALDI-TOF/MS. OBJECTIVES: We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. METHODS: We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/ testing a panel of mass spectrum produced with strains genotypically identified and, 2/ comparing MALDI-TOF/MS identification to morphology-based methods...
May 15, 2018: Mycoses
Michael Cannon, Deepa Khambe, Malgorzata Klukowska, David L Ramsey, Melanie Miner, Tom Huggins, Donald J White
OBJECTIVES: The clinical effects of stannous fluoride (SnF2) dentifrice in reducing symptoms of gingivitis and reducing the virulence of subgingival plaque through suppression of activation of gene expression in toll receptor based reporter cells were previously reported. This study expanded analysis of the clinical study to include evaluation of dentifrice effects on salivary metabolites using 1H Nuclear Magnetic Resonance (1H NMR) systems biology-based metabonomics. METHODS: The clinical design was reported previously (J Clin Dent2017;28:16-26)...
March 2018: Journal of Clinical Dentistry
Xin Hou, Meng Xiao, Sharon Ca Chen, Fanrong Kong, He Wang, Xin Fan, Yu-Pei Zhao, Ying-Chun Xu
AIM: Distinction of species within the Candida glabrata complex (i.e., C. glabrata sensu stricto, Candida nivariensis and Candida bracarensis) is relevant for epidemiological purposes and antifungal management. MATERIALS & METHODS: Two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems were comprehensively evaluated for the identification of fungi within this complex. RESULTS: None of the species (C...
May 10, 2018: Future Microbiology
Jade L L Teng, Ying Tang, Samson S Y Wong, Jordan Y H Fong, Zhe Zhao, Chun-Pong Wong, Jonathan H K Chen, Antonio H Y Ngan, Alan K L Wu, Kitty S C Fung, Tak-Lun Que, Susanna K P Lau, Patrick C Y Woo
Although Tsukamurella infections have been increasingly reported in Europe, Asia, America, and Africa, indicating that diseases caused by this group of bacteria are emerging in a global scale, species identification within this genus is difficult in most clinical microbiology laboratories. Recently, we showed that groEL gene sequencing is useful for identification of all existing Tsukamurella species. Nevertheless, PCR sequencing is still considered expensive, time-consuming, and technically demanding, and therefore is yet to be incorporated as a routine identification method in clinical laboratories...
May 9, 2018: Emerging Microbes & Infections
Sang Hee Ahn, Nohyun Lee, Changhoon Choi, Sung Won Shin, Youngyih Han, Hee Chul Park
We investigated the feasibility of using multifunctional Fe3O4/TaOx (core/shell) nanoparticles, developed for use in contrast agents for computed tomography (CT) and magnetic resonance imaging (MRI), as dose-enhancing radiosensitizers. First, to verify the detectability of Fe3O4/TaOx nanoparticles in imaging, in vivo tests were conducted. Approximately 600 mg/kg of 19-nm-diameter Fe3O4/TaOx nanoparticles dispersed in phosphate-buffered saline was injected into the tail vein of six Balb/c mice used as tumour (4T1 mammary carcinoma cell) models...
May 4, 2018: Physics in Medicine and Biology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"