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https://www.readbyqxmd.com/read/28100598/molecular-and-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-characterization-of-clinically-significant-melanized-fungi-in-india
#1
Ashutosh Singh, Pradeep Kumar Singh, Anil Kumar, Jagdish Chander, Geetika Khanna, Pradip Roy, Jacques F Meis, Anuradha Chowdhary
Melanized or black fungi are a heterogeneous group of fungi causing cutaneous to systemic diseases with high mortality. These fungi are rarely reported as agent of human infections primarily due to difficulties in their classical identification. In this study we examined using molecular methods and MALDI-TOF MS the diversity of melanized fungi (MF) isolated from patients in 19 medical centers in India. Overall, during a 4-year period, 718 (5.3%) clinical specimens yielded MF. Of these, 72 (10%) isolates had clinical significance and were identified primarily by sequencing the ITS and LSU regions...
January 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#2
Xinxin Lu, Yanfei Huang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Jinglin Wang
BACKGROUND: filamentous fungi are the most important pathogens causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time-consuming and expertise-dependent. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed to identify various fungi, its performance for the identification of FRS fungi is less clear...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28064071/enhanced-efficiency-of-solid-state-nmr-investigations-of-energy-materials-using-an-external-automatic-tuning-matching-eatm-robot
#3
Oliver Pecher, David M Halat, Jeongjae Lee, Zigeng Liu, Kent J Griffith, Marco Braun, Clare P Grey
We have developed and explored an external automatic tuning/matching (eATM) robot that can be attached to commercial and/or home-built magic angle spinning (MAS) or static nuclear magnetic resonance (NMR) probeheads. Complete synchronization and automation with Bruker and Tecmag spectrometers is ensured via transistor-transistor-logic (TTL) signals. The eATM robot enables an automated "on-the-fly" re-calibration of the radio frequency (rf) carrier frequency, which is beneficial whenever tuning/matching of the resonance circuit is required, e...
December 23, 2016: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28056838/first-case-report-of-myopericarditis-linked-to-campylobacter-coli-enterocolitis
#4
Cameron R M Moffatt, Soniah B Moloi, Karina J Kennedy
BACKGROUND: Campylobacter spp. are a common cause of mostly self-limiting enterocolitis. Although rare, pericarditis and myopericarditis have been increasingly documented as complications following campylobacteriosis. Such cases have occurred predominantly in younger males, and involved a single causative species, namely Campylobacter jejuni. We report the first case of myopericarditis following Campylobacter coli enterocolitis, with illness occurring in an immunocompetent middle-aged female...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28050983/wear-resistance-of-bulk-fill-composite-resin-restorative-materials-polymerized-under-different-curing-intensities
#5
Fahad Alkhudhairy
INTRODUCTION: The aim of this study was to assess the wear resistance of four bulk-fill composite resin restorative materials cured using high- and low-intensity lights. MATERIALS AND METHODS: Twenty-four samples were prepared from each composite resin material (Tetric N-Ceram, SonicFill, Smart Dentin Replacement, Filtek Bulk-Fill) resulting in a total of 96 samples; they were placed into a mold in a single increment. All of the 96 samples were cured using the Bluephase N light curing unit for 20 seconds...
January 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28046759/su-f-i-64-comparison-of-lipid-contents-using-steam-and-press-with-in-vivo-high-resolution-spectra-at-9-4-t
#6
K-H Song, S-I Lim, C-H Yoo, B-Y Choe
PURPOSE: The objective of this study is to compare lipid contents using localized point-resolved spectroscopy (PRESS) and stimulated echo acquisition mode (STEAM) sequences to assess lipid resonances in the liver, using in vivo high-resolution spectra at 9.4 T. METHODS: The examinations were performed on a Bruker 9.4T scanner using 4-channel animal coil for higher resolution. We used PRESS (repetition time [TR]/ TE = 3500/20 ms; number of signal averages [NSA] = 128; acquisition data points = 2048) and STEAM (TR/TM/TE = 3500/10/20 ms; NSA = 128; acquisition data points = 2048)...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28041734/identification-of-bovine-associated-coagulase-negative-staphylococci-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-using-a-direct-transfer-protocol
#7
M Cameron, H W Barkema, J De Buck, S De Vliegher, M Chaffer, J Lewis, G P Keefe
This study evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) for the identification of bovine-associated coagulase-negative staphylococci (CNS), a heterogeneous group of different species. Additionally, we aimed to expand the MALDI-ToF MS database with new reference spectra as required to fill the gaps within the existing commercial spectral library. A total of 258 isolates of CNS were used in the study, covering 16 different CNS species. The majority of the isolates were previously identified by rpoB gene sequencing (n = 219), and the remainder were identified by sequencing of 16S rRNA, hsp60, or both rpoB and hsp60...
December 29, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/28001159/effect-of-cu-ii-on-in-vitro-glycation-of-human-serum-albumin-by-methylglyoxal-a-lc-ms-based-proteomic-approach
#8
Alejandra Sarahi Ramirez Segovia, Kazimierz Wrobel, Francisco Javier Acevedo Aguilar, Alma Rosa Corrales Escobosa, Katarzyna Wrobel
It has been reported that glycation of human serum albumin (HSA) changes its capability for copper binding whereas the increase of free copper might have an impact on protein glycation - a key process in diabetes progression. In this work, proteomic analysis of non-glycated HSA and HSA glycated with methylglyoxal (MGo) in the absence or in the presence of Cu(ii) (0.1; 1.0; 5.0 mg Cu L(-1)) has been undertaken. Trypsin hydrolysates were subjected to capillary HPLC-ESI-QTOF-MS and MS/MS. Raw data were analyzed using two proteomic platforms: MaxQuant () and ProteinScape (Bruker)...
December 21, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27976288/bruker-pharmapulse-hts-fast-drug-candidate-screening-with-maldi-tof-ms
#9
S Dalby, J Fuchser, M Hamester
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27960108/a-technique-for-evaluating-bone-ingrowth-into-3d-printed-porous-ti6al4v-implants-accurately-using-x-ray-micro-computed-tomography-and-histomorphometry
#10
Anders Palmquist, Furqan A Shah, Lena Emanuelsson, Omar Omar, Felicia Suska
This paper investigates the application of X-ray micro-computed tomography (micro-CT) to accurately evaluate bone formation within 3D printed, porous Ti6Al4V implants manufactured using Electron Beam Melting (EBM), retrieved after six months of healing in sheep femur and tibia. All samples were scanned twice (i.e., before and after resin embedding), using fast, low-resolution scans (Skyscan 1172; Bruker micro-CT, Kontich, Belgium), and were analysed by 2D and 3D morphometry. The main questions posed were: (i) Can low resolution, fast scans provide morphometric data of bone formed inside (and around) metal implants with a complex, open-pore architecture?, (ii) Can micro-CT be used to accurately quantify both the bone area (BA) and bone-implant contact (BIC)?, (iii) What degree of error is introduced in the quantitative data by varying the threshold values?, and (iv) Does resin embedding influence the accuracy of the analysis? To validate the accuracy of micro-CT measurements, each data set was correlated with a corresponding centrally cut histological section...
November 21, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27936061/in-vivo-measurements-of-t2-relaxation-time-of-mouse-lungs-during-inspiration-and-expiration
#11
Lars E Olsson, Paul D Hockings
PURPOSE: The interest in measurements of magnetic resonance imaging relaxation times, T1, T2, T2*, with intention to characterize healthy and diseased lungs has increased recently. Animal studies play an important role in this context providing models for understanding and linking the measured relaxation time changes to the underlying physiology or disease. The aim of this work was to study how the measured transversal relaxation time (T2) in healthy lungs is affected by normal respiration in mouse...
2016: PloS One
https://www.readbyqxmd.com/read/27923670/early-and-progressive-microstructural-brain-changes-in-mice-overexpressing-human-%C3%AE-synuclein-detected-by-diffusion-kurtosis-imaging
#12
Amit Khairnar, Jana Ruda-Kucerova, Nikoletta Szabó, Eva Drazanova, Anas Arab, Birgit Hutter-Paier, Joerg Neddens, Peter Latta, Zenon Starcuk, Irena Rektorova
Diffusion kurtosis imaging (DKI) is sensitive in detecting α-Synuclein (α-Syn) accumulation-associated microstructural changes at late stages of the pathology in α-Syn overexpressing TNWT-61 mice. The aim of this study was to perform DKI in young TNWT-61 mice when α-Syn starts to accumulate and to compare the imaging results with an analysis of motor and memory impairment and α-Syn levels. Three-month-old (3mo) and six-month-old (6mo) mice underwent DKI scanning using the Bruker Avance 9.4T magnetic resonance imaging system...
December 5, 2016: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/27919649/maldi-tof-ms-identification-of-species-from-the-acinetobacter-baumannii-ab-group-revisited-inclusion-of-the-novel-a-%C3%A2-seifertii-and-a-%C3%A2-dijkshoorniae-species
#13
M Marí-Almirall, C Cosgaya, P G Higgins, A Van Assche, M Telli, G Huys, B Lievens, H Seifert, L Dijkshoorn, I Roca, Jordi Vila
OBJECTIVES: Rapid identification of Acinetobacter species is critical as members of the A. baumannii (Ab) group differ in antibiotic susceptibility and clinical outcomes. A. baumannii, A. pittii, and A. nosocomialis can be identified by MALDI-TOF/MS, while the novel species A. seifertii and A. dijkshoorniae cannot. Low identification rates for A. nosocomialis also have been reported. We evaluated the use of MALDI-TOF/MS to identify isolates of A. seifertii and A. dijkshoorniae and revisited the identification of A...
December 3, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27895585/feto-maternal-trafficking-of-exosomes-in-murine-pregnancy-models
#14
Samantha Sheller-Miller, Jun Lei, George Saade, Carlos Salomon, Irina Burd, Ramkumar Menon
Timing and initiation of labor are well-orchestrated by signals communicated between the fetal and maternal compartments; however, how these signals are communicated is not completely understood. Fetal exosomes, intercellular signaling vesicles, may play a key role in the process. The objective of this study was to evaluate exosome trafficking in vivo from fetal to maternal compartments. Pregnant CD-1 mice were intra-amniotically injected on gestational day 16 and 17 with exosomes isolated from primary human amnion epithelial cells fluorescently labeled with the lipophilic dye 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR)...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27891048/metabolic-signatures-of-lung-cancer-in-sputum-and-exhaled-breath-condensate-detected-by-1-h-magnetic-resonance-spectroscopy-a-feasibility-study
#15
Naseer Ahmed, Tedros Bezabeh, Omkar B Ijare, Renelle Myers, Reem Alomran, Michel Aliani, Zoann Nugent, Shantanu Banerji, Julian Kim, Gefei Qing, Zoheir Bshouty
OBJECTIVES: Lung cancer is one of the most lethal cancers. Currently, there are no biomarkers for early detection, monitoring treatment response, and detecting recurrent lung cancer. We undertook this study to determine if (1)H magnetic resonance spectroscopy (MRS) of sputum and exhaled breath condensate (EBC), as a noninvasive tool, can identify metabolic biomarkers of lung cancer. MATERIALS AND METHODS: Sputum and EBC samples were collected from 20 patients, comprising patients with pathologically confirmed non-small cell lung cancer (n = 10) and patients with benign respiratory conditions (n = 10)...
2016: Magnetic Resonance Insights
https://www.readbyqxmd.com/read/27868301/exploring-maldi-tof-ms-approach-for-a-rapid-identification-of-mycobacterium-avium-subsp-paratuberculosis-field-isolates
#16
M Ricchi, A Mazzarelli, A Piscini, A Di Caro, A Cannas, S Leo, S Russo, N Arrigoni
AIMS: The aim of the study was to explore the suitability of MALDI-TOF MS for a rapid and correct identification of Mycobacterium avium subsp. paratuberculosis (MAP) field isolates. METHODS AND RESULTS: MALDI-TOF MS approach is becoming one of the most popular tests for the identification of intact bacterial cells which has been shown to be fast and reliable. For this purpose, 36 MAP field isolates were analysed through MALDI-TOF MS and the spectra compared with two different databases: one provided by the vendor of the system employed (Biotyper Version 3...
November 21, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27863949/imipenem-avibactam-a-novel-combination-for-the-rapid-detection-of-carbapenemase-activity-in-enterobacteriaceae-and-acinetobacter-baumannii-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#17
Marina Oviaño, Germán Bou
In the present study, we propose a novel matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for detecting carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii. For this, we analyzed a series of 131 isolates. Among them, a total of 115 Enterobacteriaceae: 79 of them carrying a carbapenemase enzyme (15blaKPC, 7blaNDM, 11blaIMP, 12blaVIM, and 34blaOXA-48) and 16 A. baumannii isolates: 15 of them carrying carbapenemases (10blaOXA-23, 2blaOXA-58, 2blaOXA-24, and 1blaOXA-237)...
February 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27837329/highly-crosslinked-polyethylene-a-safe-alternative-to-conventional-polyethylene-for-dual-mobility-cup-mobile-component-a-biomechanical-validation
#18
Matthieu Malatray, Jean-Paul Roux, Stanislas Gunst, Vincent Pibarot, Julien Wegrzyn
PURPOSE: Dual mobility cup (DMC) consists of a cobalt-chromium (CoCr) alloy cup articulated with a polyethylene (PE) mobile component capturing the femoral head in force using a snap-fit technique. This biomechanical study was the first to evaluate and compare the generation of cracks in the retentive area of DMC mobile components made of highly crosslinked PE (XLPE) or conventional ultra-high molecular weight PE (UHMWPE). METHODS: Eighty mobile components designed for a 52-mm diameter Symbol® DMC (Dedienne Santé, Mauguio, France) and a 28-mm diameter femoral head were analyzed...
November 11, 2016: International Orthopaedics
https://www.readbyqxmd.com/read/27798637/the-technical-and-biological-reproducibility-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-based-typing-employment-of-bioinformatics-in-a-multicenter-study
#19
Michael Oberle, Nadia Wohlwend, Daniel Jonas, Florian P Maurer, Geraldine Jost, Sarah Tschudin-Sutter, Katleen Vranckx, Adrian Egli
BACKGROUND: The technical, biological, and inter-center reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI TOF MS) typing data has not yet been explored. The aim of this study is to compare typing data from multiple centers employing bioinformatics using bacterial strains from two past outbreaks and non-related strains. MATERIAL/METHODS: Participants received twelve extended spectrum betalactamase-producing E. coli isolates and followed the same standard operating procedure (SOP) including a full-protein extraction protocol...
2016: PloS One
https://www.readbyqxmd.com/read/27796646/streptococcus-bovis-new-taxonomy-does-subspecies-distinction-matter
#20
E Ben-Chetrit, Y Wiener-Well, L Kashat, A M Yinnon, M V Assous
Bacteremia with Streptococcus bovis/equinus complex strains is associated with hepatobiliary disease, colorectal lesions (CL), and infective endocarditis (IE). This study addressed the clinical significance of subspecies distinction of previously designated S. bovis blood culture isolates according to the updated nomenclature. During 2002-2013, all blood culture isolates previously designated as S. bovis were recultured and identified using 16S rRNA gene sequencing and MALDI-TOF (Bruker BioTyper and Vitek MS, bioMérieux)...
October 28, 2016: European Journal of Clinical Microbiology & Infectious Diseases
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