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https://www.readbyqxmd.com/read/28514339/-increased-oral-fluid-remineraling-function-by-endogenous-and-exogenous-saturation-methods-of-its-mineral-complexes
#1
O G Avraamova, Yu A Ippolitov, Ya A Plotnikova, P V Seredin, D V Goloshapov, E O Aloshina
RELEVANCE: One of the most common problems in modern caries prevention is the development of techniques to increase tooth resistance to the effects of cariogenic factors. Therefore, research is needed to study the chemical composition of tissues like teeth and body fluids surrounding the tooth to prevent demineralization process. The purpose of determining the effectiveness of work- and correlation relationship between endo saturation exogenous methods oral liquid mineral complexes. MATERIAL AND METHODS: In 95 patients analyzed the chemical composition and structure of the oral fluid by IR spectroscopy...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/28505220/multicenter-evaluation-of-the-bruker-maldi-biotyper-ca-system-for-the-identification-of-clinically-important-bacteria-and-yeasts
#2
Deborah A Wilson, Stephen Young, Karen Timm, Susan Novak-Weekley, Elizabeth M Marlowe, Neil Madisen, Jennifer L Lillie, Nathan A Ledeboer, Rebecca Smith, Josh Hyke, Christen Griego-Fullbright, Patricia Jim, Paul A Granato, Matthew L Faron, Joven Cumpio, Blake W Buchan, Gary W Procop
Objectives: A report on the multicenter evaluation of the Bruker MALDI Biotyper CA System (MBT-CA; Bruker Daltonics, Billerica, MA) for the identification of clinically important bacteria and yeasts. Methods: In total, 4,399 isolates of medically important bacteria and yeasts were assessed in the MBT-CA. These included 2,262 aerobic gram-positive (AGP) bacteria, 792 aerobic gram-negative (AGN) bacteria 530 anaerobic (AnA) bacteria, and 815 yeasts (YSTs). Three processing methods were assesed...
May 13, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28500917/using-rapid-scan-epr-to-improve-the-detection-limit-of-quantitative-epr-by-more-than-one-order-of-magnitude
#3
J Möser, K Lips, M Tseytlin, G R Eaton, S S Eaton, A Schnegg
X-band rapid-scan EPR was implemented on a commercially available Bruker ELEXSYS E580 spectrometer. Room temperature rapid-scan and continuous-wave EPR spectra were recorded for amorphous silicon powder samples. By comparing the resulting signal intensities the feasibility of performing quantitative rapid-scan EPR is demonstrated. For different hydrogenated amorphous silicon samples, rapid-scan EPR results in signal-to-noise improvements by factors between 10 and 50. Rapid-scan EPR is thus capable of improving the detection limit of quantitative EPR by at least one order of magnitude...
April 17, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28490491/comparison-of-the-vitek-ms-and-bruker-maldi-tof-ms-mass-spectrometry-systems-for-the-identification-of-rhodococcus-equi-and-dietzia-spp
#4
Carlos Ruiz de Alegría Puig, Lilian Pilares, Francesc Marco, Jordi Vila, Luis Martínez-Martínez, Jesús Navas
Rhodococcus equi causes pyogranulomatous pneumonia in domesticated animals and immunocompromised humans. Dietzia spp. are environmental bacteria that have been rarely associated with human infections. R. equi and Dietzia spp. are closely related actinomycetes. Phenotypic discrimination between R. equi and Dietzia on the basis of their Gram stain morphology and colony appearance is problematic. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable and cost-effective method for identification of a wide variety of microorganisms...
May 10, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28487677/accurate-differentiation-of-streptococcus-pneumoniae-from-other-species-within-the-streptococcus-mitis-group-by-peak-analysis-using-maldi-tof-ms
#5
Mercedes Marín, Emilia Cercenado, Carlos Sánchez-Carrillo, Adrián Ruiz, Álvaro Gómez González, Belén Rodríguez-Sánchez, Emilio Bouza
Despite the benefits of MALDI-TOF MS technology (Matrix-Assisted Laser Desorption-Ionization Time-Of-Flight Mass Spectrometry) reported worldwide and the continuous improving of the available databases, discrimination between Streptococcus pneumoniae and closely related species within the Streptococcus mitis group (SMG) using this methodology has been suboptimal. However, the accurate identification at the species level of this group of microorganisms is important for the appropriate management of infected patients...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28470986/nickel-ii-assisted-enantiomeric-differentiation-and-quantitation-of-tadalafil-by-direct-electrospray-ionization-mass-spectrometry
#6
Lu Wang, Yali Wang, Yunfeng Chai, Yu Kang, Cuirong Sun, Su Zeng
A facile method based on electrospray mass spectrometry was established and validated for the differentiation of enantiomeric Tadalafil isomers without using chiral chromatographic separation. The enantiomers were coupled with a chiral selector to form diastereomeric complex ions. Nickel-Tadalafil complexes, [Ni(II) (Tadalafil)(L-Trp)-H](+) , produced a characteristic fragment ion at m/z 524 by loss of 1-methyl-1, 6-dihydropyrazine-2, 5-dione (MDD) via collision-induced dissociation (CID). The relative abundance of this fragment ion to the precursor contributed to differentiate Tadalafil enantiomers, and energy-resolved product-ion spectra were applied to determine molar composition of Tadalafil in the mixture (R, R and S, S) as well...
May 4, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28455886/optimization-of-electrospray-ionization-conditions-to-enhance-formation-of-doubly-protonated-peptide-ions-with-and-without-addition-of-chromium-iii
#7
Juliette J Commodore, Xinyao Jing, Carolyn J Cassady
RATIONALE: Production of multiply protonated ions by electrospray ionization (ESI) is important to the analysis of peptides by mass spectrometry. For small neutral and acidic peptides, addition of chromium(III) greatly increases the intensity of doubly protonated ions. The current study examines instrumental and solution parameters that maximize peptide ion charge by ESI. METHODS: The neutral and basic heptapeptides AAAAAAA (A7) and AAAKAAA (A3KA3) were used as test compounds and electrosprayed from a solution containing chromium(III) nitrate at a peptide to metal molar ratio of 1:10...
April 29, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28449877/two-stage-algorithm-vs-commonly-used-approaches-for-the-suspect-screening-of-complex-environmental-samples-analyzed-via-liquid-chromatography-high-resolution-time-of-flight-mass-spectroscopy-a-test-study
#8
Saer Samanipour, Jose A Baz-Lomba, Nikiforos A Alygizakis, Malcolm J Reid, Nikolaos S Thomaidis, Kevin V Thomas
LC-HR-QTOF-MS recently has become a commonly used approach for the analysis of complex samples. However, identification of small organic molecules in complex samples with the highest level of confidence is a challenging task. Here we report on the implementation of a two stage algorithm for LC-HR-QTOF-MS datasets. We compared the performances of the two stage algorithm, implemented via NIVA_MZ_Analyzer™, with two commonly used approaches (i.e. feature detection and XIC peak picking, implemented via UNIFI by Waters and TASQ by Bruker, respectively) for the suspect analysis of four influent wastewater samples...
April 20, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28444315/towards-the-early-detection-of-%C3%AE-lactamase-producing-enterobacteriaceae-by-maldi-tof-ms-analysis
#9
Marina Oviaño, Marta Gómara, Maria José Barba, María José Revillo, Luis Pedro Barbeyto, Germán Bou
Objectives: Development of a novel MALDI-TOF MS-based method for the rapid detection of ESBL-producing Enterobacteriaceae. Methods: The method was evaluated in terms of sensitivity, specificity and turnaround time regarding the antibiotic used (cefotaxime, ceftazidime, ceftriaxone, cefpodoxime or cefepime) and the performance of the automated MBT STAR-BL software (Bruker Daltonik GmbH, Germany) relative to qualitative interpretation of spectra for detecting β-lactamase resistance by MALDI-TOF MS (Bruker Daltonik) in a collection of 11 isogenic Escherichia coli control strains expressing different β-lactamases...
April 24, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28443694/fractal-features-and-surface-micromorphology-of-unworn-surfaces-of-rigid-gas-permeable-contact-lenses
#10
Ştefan Ţălu, Miroslaw Bramowicz, Slawomir Kulesza, Ilenia Fiorillo, Stefano Giovanzana
PURPOSE: The aim of this exploratory study was to investigate the micromorphology of surfaces of rigid gas permeable (RGP) contact lenses (CLs) using atomic force microscopy (AFM) followed by fractal analysis. MATERIALS AND METHODS: In order to characterize in a quantitative manner the micromorphology of surfaces of new and unworn RGP CLs made of twelve different materials, AFM was taken and then analyzed using fractal methods. Surface topography was sampled in an intermittent-contact mode in air, on square areas of 5 × 5 µm(2) (MultiMode with Nanoscope V (Bruker)...
April 26, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28440576/new-cascarosides-from-rhamnus-purshiana-and-fragmentation-studies-of-the-class-by-it-ms
#11
Daniel P Demarque, Danielle R Pinho, Daniel R Callejon, Gibson G de Oliveira, Denise B Silva, Carlos A Carollo, Norberto P Lopes
RATIONALE: Anthrone and oxanthrone are important anthraquinone derivatives present in medicinal plants and are used in therapeutics as laxatives. Some of these plants need to be stored at least one year before they can be used in order to oxidize anthrones into oxanthrones, so to avoid severe diarrhea and dehydration. Therefore, this work aimed to characterize fragmentation reactions between these anthraquinones to provide an easy way to differentiate between the two classes, since it is necessary and important to discriminate and identify these derivatives in laxative plants and phytotherapic drugs...
April 25, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28438498/performance-of-maldi-tof-ms-for-identification-of-oral-prevotella-species
#12
Mervi Gürsoy, Inka Harju, Jaakko Matomäki, Anne Bryk, Eija Könönen
During the past decade, the clinically relevant genus Prevotella has expanded considerably. Prevotella species can be isolated from nearly all types of oral infections but also from various non-oral infections. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been introduced in clinical microbiology laboratories as a convenient method for identifying bacterial isolates from clinical specimens. Here we tested the diagnostic accuracy of a total of 123 oral Prevotella isolates, selected based on their biochemical profile, by Bruker MALDI-TOF MS...
April 21, 2017: Anaerobe
https://www.readbyqxmd.com/read/28431114/molecular-characterization-of-cryptococcus-neoformans-isolated-from-the-environment-in-beijing-china
#13
Hongtao Dou, Huizhu Wang, Shaowei Xie, Xinxin Chen, Zhipeng Xu, Yingchun Xu
The molecular type of environmental Cryptococcus neoformans in Beijing was not clear. Our study aims to reveal the molecular characterization of C. neoformans complex from environment in Beijing, China. A total of 435 samples of pigeon droppings from 11 different homes in Beijing were collected from August to November in 2015. Pigeon droppings were inoculated onto caffeic acid cornmeal agar (CACA) to screen C. neoformans complex. Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed for species identification...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28412936/-1-h-nmr-analysis-of-feces-new-possibilities-in-the-helminthes-infections-research
#14
Sarantos Kostidis, Daria Kokova, Natalia Dementeva, Irina V Saltykova, Hye Kyong Kim, Young Hae Choi, Oleg A Mayboroda
BACKGROUND: Analysis of the stool samples is an essential part of routine diagnostics of the helminthes infections. However, the standard methods such Kato and Kato-Katz utilize only a fraction of the information available. Here we present a method based on the nuclear magnetic resonance spectroscopy (NMR) which could be auxiliary to the standard procedures by evaluating the complex metabolic profiles (or phenotypes) of the samples. METHOD: The samples were collected over the period of June-July 2015, frozen at -20 °C at the site of collection and transferred within four hours for the permanent storage at -80 °C...
April 17, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28412013/investigation-of-the-imaging-characteristics-of-the-albira-ii-small-animal-pet-system-for-18-f-68-ga-and-64-cu
#15
Ali Asgar Attarwala, Yvonne Wanjiku Karanja, Deni Hardiansyah, Chiara Romanó, Mareike Roscher, Björn Wängler, Gerhard Glatting
AIM: In this study the performance characteristics of the Albira II PET sub-system and the response of the system for the following radionuclides (18)F, (68)Ga and (64)Cu was analyzed. MATERIALS AND METHODS: The Albira II tri-modal system (Bruker BioSpin MRI GmbH, Ettlingen, Germany) is a pre-clinical device for PET, SPECT and CT. The PET sub-system uses single continuous crystal detectors of lutetium yttrium orthosilicate (LYSO). The detector assembly consists of three rings of 8 detector modules...
April 12, 2017: Zeitschrift Für Medizinische Physik
https://www.readbyqxmd.com/read/28400760/aeromonas-diversity-and-antimicrobial-susceptibility-in-freshwater-an-attempt-to-set-generic-epidemiological-cut-off-values
#16
Sandrine Baron, Sophie A Granier, Emeline Larvor, Eric Jouy, Maelan Cineux, Amandine Wilhelm, Benoit Gassilloud, Sophie Le Bouquin, Isabelle Kempf, Claire Chauvin
The importance of the role of environment in the dissemination of antimicrobial resistant bacteria is now well recognized. Thus, bacterial indicators to monitor the phenomena are required. The Aeromonas genus is autochthonous in the aquatic environment and easy to detect in any water type, such as freshwater, or wastewater. These microorganisms are also causing infections in humans and animals (including fish). Furthermore, as Aeromonas spp. is able to acquire antimicrobial resistance mechanisms, it is candidate for indicator bacteria to follow antimicrobial resistance dissemination in aquatic environments...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28397475/evaluation-of-maldi-tof-ms-for-the-identification-of-yeast-isolates-causing-bloodstream-infection
#17
Ozge Turhan, Betil Ozhak-Baysan, Oscar Zaragoza Hernandez, Halil Er, Gozde Ongut, Dilara Ogunc, Dilek Colak, Manuel Cuenca-Estrella
BACKGROUND: Infections due to Candida species are major causes of morbidity and mortality in humans, causing a diverse spectrum of clinical disease ranging from superficial and mucosal infections to invasive disease. Several authors have demonstrated that mortality is closely linked to both timing of therapy and/or source control. The rapid identification of pathogenic species is helpful to start timely and effective antifungal therapy. The aim of this study was to assess the performance of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the correct and rapid identification of yeast isolates causing bloodstream infection...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28360188/rapid-detection-of-cfia-metallo-%C3%AE-lactamase-producing-bacteroides-fragilis-by-the-combination-of-maldi-tof-ms-and-carbanp
#18
Pak-Leung Ho, Chong-Yee Yau, Lok-Yan Ho, Jonathan H K Chen, Eileen L Y Lai, Stephanie W U Lo, Cindy W S Tse, Kin-Hung Chow
AIMS: Carbapenem resistance in Bacteroides fragilis is emerging and is mainly attributed to insertion sequence (IS)-mediated activation of the carbapenemase gene cfiA. We investigated the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and the CarbaNP assay for the rapid identification of these strains. METHODS: This study used the Bruker MALDI Biotype system and the mass spectra models generated by 20 B. fragilis reference strains (10 cfiA-positive and 10 cfiA-negative) in the ClinProTools software to identify 404 B...
March 30, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28349999/potential-of-maldi-tof-ms-as-an-alternative-approach-for-capsular-typing-streptococcus-pneumoniae-isolates
#19
Tatiana C A Pinto, Natalia S Costa, Luciana F S Castro, Rachel L Ribeiro, Ana Caroline N Botelho, Felipe P G Neves, Jose Mauro Peralta, Lucia M Teixeira
Streptococcus pneumoniae can be classified in more than 90 capsular types, as traditionally determined by serological methods and more recently by PCR-based techniques. Such methods, however, can be expensive, laborious or unable to accurately discriminate among certain serotypes. Therefore, determination of capsular types, although extremely important for epidemiological purposes and for estimating the impact of pneumococcal conjugate vaccines, is mainly restricted to research laboratories, being rarely performed in the clinical setting...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28327698/accuracy-and-reliability-of-different-cone-beam-computed-tomography-cbct-devices-for-structural-analysis-of-alveolar-bone-in-comparison-with-multislice-ct-and-micro-ct
#20
Jeroen Van Dessel, Laura Ferreira Pinheiro Nicolielo, Yan Huang, Walter Coudyzer, Benjamin Salmon, Ivo Lambrichts, Reinhilde Jacobs
OBJECTIVE: The aim of this study was to assess whether cone beam computed tomography (CBCT) may be used for clinically reliable alveolar bone quality assessment in comparison to its clinical alternatives, multislice computed tomography and the gold standard (micro-CT). MATERIALS AND METHODS: Six dentate mandibular bone samples were scanned with seven CBCT devices (ProMax 3D Max, NewTom GiANO, Cranex 3D, 3D Accuitomo 170, Carestream 9300, Scanora 3D, I-CAT Next generation), one micro-CT scanner (SkyScan 1174) and one MSCT machine (Somatom Definition Flash) using two protocols (standard and high-resolution)...
2017: European Journal of Oral Implantology
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