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https://www.readbyqxmd.com/read/29656124/development-of-an-improved-rapid-bacpro%C3%A2-protocol-and-a-method-for-direct-identification-from-blood-culture-positive-bottles-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#1
Takatoshi Yonezawa, Tomohisa Watari, Kazuho Ashizawa, Daisuke Hanada, Takako Yanagiya, Naoki Watanabe, Takashi Terada, Yutaka Tomoda, Satoshi Fujii
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been incorporated into pathogenic bacterial identification methods and has improved their rapidity. Various methods have been reported to directly identify bacteria with MALDI-TOF MS by pretreating culture medium in blood culture bottles. Rapid BACpro® (Nittobo Medical Co., Ltd.) is a pretreatment kit for effective collection of bacteria with cationic copolymers. However, the Rapid BACpro® pretreatment kit is adapted only for MALDI Biotyper (Bruker Daltonics K...
April 12, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29644206/growing-asymptomatic-nodule-on-the-ear
#2
Alyssa Miceli, Ann Church, Sarah Ferrer-Bruker
No abstract text is available yet for this article.
March 2018: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/29643036/-quantitative-magnetic-resonance-imaging-of-brain-iron-deposition-comparison-between-quantitative-susceptibility-mapping-and-transverse-relaxation-rate-r2-mapping
#3
Ji-Jing Guan, Yan-Qiu Feng
OBJECTIVE: To evaluate the accuracy and sensitivity of quantitative susceptibility mapping (QSM) and transverse relaxation rate (R2*) mapping in the measurement of brain iron deposition. METHODS: Super paramagnetic iron oxide (SPIO) phantoms and mouse models of Parkinson's disease (PD) related to iron deposition in the substantia nigra (SN) underwent 7.0 T magnetic resonance (MR) scans (Bruker, 70/16) with a multi-echo 3D gradient echo sequence, and the acquired data were processed to obtain QSM and R2*...
March 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29626622/bacteroides-fragilis-a-whole-maldi-based-workflow-from-identification-to-confirmation-of-carbapenemase-production-for-routine-laboratories
#4
M Cordovana, M Kostrzewa, J Sóki, E Witt, S Ambretti, A B Pranada
Bacteroides fragilis is a frequent anaerobic pathogen and can cause severe infections. Resistance to carbapenems, associated with the cfiA gene encoded carbapenemase, represents an emerging problem. To date, no rapid methods are available to detect and confirm this resistance mechanism in routine laboratories, and the missed recognition of carbapenemase-producing strains can lead to therapeutic failures. In this study we have investigated a whole MALDI-TOF MS-based workflow to detect carbapenemase-producing B...
April 4, 2018: Anaerobe
https://www.readbyqxmd.com/read/29626448/zoledronic-acid-induces-site-specific-structural-changes-and-decreases-vascular-area-in-the-alveolar-bone
#5
Mariana Quirino Silveira Soares, Jeroen Van Dessel, Reinhilde Jacobs, Paulo Sérgio da Silva Santos, Tania Mary Cestari, Gustavo Pompermaier Garlet, Marco Antonio Hungaro Duarte, Thaís Sumie Nozu Imada, Ivo Lambrichts, Izabel Regina Fischer Rubira-Bullen
PURPOSE: The aim was to assess the effect of a relevant regimen of zoledronic acid (ZA) treatment for the study of bisphosphonate-related osteonecrosis of the jaw on alveolar bone microstructure and vasculature. A sub-objective was to use 3-dimensional imaging to describe site-specific changes induced by ZA in the alveolar bone. MATERIALS AND METHODS: Five Wistar rats received ZA (0.6 mg/kg) and five (controls) received saline solution in the same volume. The compounds were administered intraperitoneally in 5 doses every 28 days...
March 15, 2018: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29604726/a-new-device-for-high-temperature-in-situ-gisaxs-measurements
#6
Gerhard Fritz-Popovski, Sabine C Bodner, Florentyna Sosada-Ludwikowska, Günther A Maier, Roland Morak, Livia Chitu, Lutz Bruegemann, Joachim Lange, Hans-Georg Krane, Oskar Paris
A heating stage originally designed for diffraction experiments is implemented into a Bruker NANOSTAR instrument for in situ grazing incidence small-angle x-ray scattering experiments. A controlled atmosphere is provided by a dome separating the sample environment from the evacuated scattering instrument. This dome is double shelled in order to enable cooling water to flow through it. A mesoporous silica film templated by a self-assembled block copolymer system is investigated in situ during step-wise heating in air...
March 2018: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29584664/comparison-of-portable-and-bench-top-spectrometers-for-mid-infrared-diffuse-reflectance-measurements-of-soils
#7
Christopher Hutengs, Bernard Ludwig, András Jung, Andreas Eisele, Michael Vohland
Mid-infrared (MIR) spectroscopy has received widespread interest as a method to complement traditional soil analysis. Recently available portable MIR spectrometers additionally offer potential for on-site applications, given sufficient spectral data quality. We therefore tested the performance of the Agilent 4300 Handheld FTIR (DRIFT spectra) in comparison to a Bruker Tensor 27 bench-top instrument in terms of (i) spectral quality and measurement noise quantified by wavelet analysis; (ii) accuracy of partial least squares (PLS) calibrations for soil organic carbon (SOC), total nitrogen (N), pH, clay and sand content with a repeated cross-validation analysis; and (iii) key spectral regions for these soil properties identified with a Monte Carlo spectral variable selection approach...
March 27, 2018: Sensors
https://www.readbyqxmd.com/read/29576776/feasibility-of-micro-computed-tomography-to-detect-and-classify-proximal-caries-lesions-in-vitro
#8
Karla Rovaris, Liana Matos Ferreira, Thiago Oliveira Sousa, Leonardo Vieira Peroni, Deborah Queiroz Freitas, Ann Wenzel, Francisco Haiter-Neto
Background: Micro-computed tomography (micro-CT) has been widely described as a nondestructive in vitro imaging method although its accuracy for caries detection is still unclear. The aim of this study was to investigate the feasibility of micro-CT to detect and classify proximal caries lesions in posterior teeth, using different protocols. Materials and Methods: In this in vitro study, crowns of 122 human teeth were scanned with Skyscan 1174 (Bruker, Kontich, Belgium) using the full-scan mode (360°)...
March 2018: Dental Research Journal
https://www.readbyqxmd.com/read/29570584/first-case-of-bacteremia-due-to-kytococcus-schroeteri-in-a-child-with-congenital-adrenal-hyperplasia
#9
Banu Bayraktar, Nazan Dalgic, Nurcan Duman, Ercüment Petmezci
Kytococcus schroeteri is a commensal organism of the human skin, may cause serious infections in immunocompromised patients. We report the first case of Kytococcus schroeteri bacteremia in a child with congenital adrenal hyperplasia (CAH), identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)(Bruker Daltonics, Germany). İdentification was confirmed by 16S rRNA gene sequencing.
March 22, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29552823/-quantitative-determination-of-febrifugine-by-proton-nuclear-magnetic-resonance-spectroscopy-with-internal-standard-method
#10
Ji-Yuan Zhang, Xiao-Rui Fan, Xiao-Qian Liu, Xiao-Wei Yu, Li-Xin Yang, Zhi-Ting Guo, Wei-Hong Feng, Zhi-Min Wang, Chun Li
A quantitative nuclear magnetic resonance method(qNMR) was established for determination of the absolute content of febrifugine. Proton nuclear magnetic resonance spectroscopy of febrifugine was obtained in DMSO-d₆ with hydroquinone as the internal standard substance on a Bruker Ascend 600 MHz superconducting nuclear resonance spectrometer at 298 K. The specific parameters were as follows: the observing frequency was 600 MHz,spectra width was 7 211 Hz, pulse width was 9.70 μs, pulse sequence was zg30,scan times was 32 and relaxation time was 2 s...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29534849/capsular-serotyping-of-haemophilus-influenzae-by-using-matrix-associated-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Noriko Takeuchi, Shunsuke Segawa, Naruhiko Ishiwada, Misako Ohkusu, Sachio Tsuchida, Mamoru Satoh, Kazuyuki Matsushita, Fumio Nomura
Haemophilus influenzae is a major pathogenic bacteria causing invasive disease, which is classified into six capsular serotypes (a-f) and non-typeable (NT) strains. Capsular serotyping of H. influenzae is traditionally determined by serological methods and more recently by PCR methods. However, these methods are time-consuming and expensive. In the present study, matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated as an alternative method for capsular serotyping of H...
March 10, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29515167/accuracy-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-identification-of-mycobacteria-a-systematic-review-and-meta-analysis
#12
Yan Cao, Lei Wang, Ping Ma, Wenting Fan, Bing Gu, Shaoqing Ju
Mycobacterium species are a significant cause of morbidity and mortality worldwide. The present study was carried out to systematically evaluate the accuracy of Matrix-assisted laser desorption ionization-time of flight mass spectroscopy (MALDI-TOF MS) for the identification of clinical pathogenic mycobacteria. After a rigid selection process, 19 articles involving 2,593 mycobacteria isolates were included. The pooled result agreed with the reference method identification for 85% of the isolates to genus level, with 71% (95% CI of 69% to 72%) correct to the species level...
March 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29514939/improved-discrimination-of-bacillus-anthracis-from-closely-related-species-in-the-bacillus-cereus-sensu-lato-group-based-on-maldi-tof-mass-spectrometry
#13
Viktoria I Pauker, Bryan R Thoma, Gregor Grass, Pauline Bleichert, Matthias Hanczaruk, Lothar Zöller, Sabine Zange
Discrimination of highly pathogenic bacteria, such as Bacillus anthracis , from closely related species based on molecular biological methods is challenging. We applied Matrix Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (MALDI-TOF MS) to a collection of B. anthracis strains and close relatives in order to significantly improve the statistical confidence of identification results for this group of bacteria. Protein mass spectra of 189 verified and diverse Bacillus strains of the Bacillus cereus sensu lato group were generated using MALDI-TOF MS and subsequently analyzed with supervised and unsupervised statistical methods, such as shrinkage discriminant analysis (SDA) and principle component analysis (PCA)...
March 7, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29501178/peptide-identifications-and-false-discovery-rates-using-different-mass-spectrometry-platforms
#14
Krishna D B Anapindi, Elena V Romanova, Bruce R Southey, Jonathan V Sweedler
Characterization of endogenous neuropeptides produced from post-translational proteolytic processing of precursor proteins is a demanding task. A variety of complex prohormone processing steps generate molecular diversity from neuropeptide prohormones, making in silico neuropeptide discovery difficult. In addition, the wide range of endogenous peptide concentrations as well as significant peptide complexity further challenge the structural characterization of neuropeptides. Liquid chromatography-mass spectrometry (MS), performed in conjunction with bioinformatics, allows for high-throughput characterization of peptides...
May 15, 2018: Talanta
https://www.readbyqxmd.com/read/29467194/identification-and-characterization-of-clostridium-difficile-genotype-st37-by-maldi-tof-mass-spectrometry
#15
Ruxin Li, Di Xiao, Jing Yang, Suju Sun, Samuel Kaplan, Zhirong Li, Yanan Niu, Cuixin Qiang, Yu Zhai, Xiaoming Wang, Xingzhen Zhao, Baoxin Zhao, Martin Welker, David H Pincus, Dazhi Jin, Mini Kamboj, Guanghui Zheng, Guojun Zhang, Jianzhong Zhang, Yi-Wei Tang, Jianhong Zhao
Clostridium difficile multilocus sequence type 37 (ST37), which mainly corresponds to ribotype 017, has been a dominant genotype circulating in China. In this study, we report the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 204 C. difficile clinical isolates including 49 ST37 and 155 non-ST37 isolates collected in China and other countries. Distribution of two major protein peaks ( m/z 3242 and 3286) was significantly different between ST37 and non-ST37 prototype strains and clinical isolates...
February 21, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29444330/increased-species-assignment-of-filamentous-fungi-using-maldi-tof-ms-coupled-with-a-simplified-sample-processing-and-an-in-house-library
#16
M E Zvezdanova, P Escribano, A Ruiz, M C Martínez-Jiménez, T Peláez, A Collazos, J Guinea, E Bouza, B Rodríguez-Sánchez
In this study we evaluated the capacity of MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) to identify clinical mould isolates. We focused on two aspects of MALDI-TOF MS identification: the sample processing and the database. Direct smearing of the sample was compared with a simplified processing consisting of mechanical lysis of the moulds followed by a protein extraction step. Both methods were applied to all isolates and the Filamentous Fungi Library 1.0 (Bruker Daltonics) was used for their identification...
February 9, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29438105/magnetic-resonance-and-photoacoustic-imaging-of-brain-tumor-mediated-by-mesenchymal-stem-cell-labeled-with-multifunctional-nanoparticle-introduced-via-carotid-artery-injection
#17
Yang Qiao, Joy Gumin, Christopher J MacLellan, Feng Gao, Richard Bouchard, Frederick F Lang, R Jason Stafford, Marites P Melancon
OBJECTIVE: To evaluate the feasibility of visualizing bone marrow-derived human mesenchymal stem cells (MSCs) labeled with a gold-coated magnetic resonance (MR)-active multifunctional nanoparticle and injected via the carotid artery for assessing the extent of MSC homing in glioma-bearing mice. MATERIALS AND METHODS: Nanoparticles containing superparamagnetic iron oxide coated with gold (SPIO@Au) with a diameter of ∼82 nm and maximum absorbance in the near infrared region were synthesized...
April 20, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29422351/comparison-of-the-identification-results-of-candida-species-obtained-by-bd-phoenix%C3%A2-and-maldi-tof-bruker-microflex-lt-biotyper-3-1
#18
Andrea P Marucco, Patricia Minervini, Gabriela V Snitman, Adriana Sorge, Liliana I Guelfand, Laura López Moral
In patients with invasive fungal infections, the accurate and rapid identification of the genus Candida is of utmost importance since antimycotic sensitivity is closely related to the species. The aim of the present study was to compare the identification results of species of the genus Candida obtained by BD Phoenix™ (Becton Dickinson [BD]) and Maldi-TOF MS (Bruker Microflex LT Biotyper 3.1). A total of 192 isolates from the strain collection belonging to the Mycology Network of the Autonomous City of Buenos Aires, Argentina, were analyzed...
February 5, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29401558/same-day-identification-and-antimicrobial-susceptibility-testing-of-bacteria-in-positive-blood-culture-broths-using-short-term-incubation-on-solid-medium-with-the-microflex-lt-vitek-ms-and-vitek2-systems
#19
Jihye Ha, Sung Kuk Hong, Geum Hee Han, Myungsook Kim, Dongeun Yong, Kyungwon Lee
BACKGROUND: Early and appropriate antibiotic treatment improves the clinical outcome of patients with septicemia; therefore, reducing the turn-around time for identification (ID) and antimicrobial susceptibility test (AST) results is essential. We established a method for rapid ID and AST using short-term incubation of positive blood culture broth samples on solid media, and evaluated its performance relative to that of the conventional method using two rapid ID systems and a rapid AST method...
May 2018: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/29400736/python-based-dynamic-scheduling-assistant-for-atmospheric-measurements-by-bruker-instruments-using-opus
#20
Alexander Geddes, John Robinson, Dan Smale
Atmospheric remote sensing by instruments such as spectrometers and interferometers often requires scheduling that is dependent on external factors, for example; time and solar (or lunar) zenith angle. Such instruments manufactured by Bruker often use the software package OPUS, which, while useful, is not flexible enough for automatic, repeated, atmospheric measurements of this nature. In this brief paper, we describe ASAP, a Python tool developed to run our network of Fourier transform interferometers in New Zealand and Antarctica...
February 1, 2018: Applied Optics
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