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Giovanni Gherardi, Claudio Palmieri, Emanuela Marini, Arianna Pompilio, Valentina Crocetta, Giovanni Di Bonaventura, Roberta Creti, Bruna Facinelli
This study aimed to retrospectively identify 22Streptococcus bovis clinical strains based on the new taxonomy, as well as to investigate their antibiotic-resistance and clonality. Strains were identified by Phoenix100 system, 16S rRNA sequencing, and two MALDI-TOF MS platforms (Bruker Biotyper, Vitek MS). Antibiotic resistance was determined both phenotypically and genotypically, and clonality was assessed by PFGE. Most of strains (63.6%) were isolated from urine, and diabetes was the most common underlying disease (31...
September 24, 2016: Diagnostic Microbiology and Infectious Disease
Tae-Hyoung Kim, Oh Joo Kweon, Hye Ryoun Kim, Mi-Kyung Lee
Recently, several studies have revealed that commercial microbial identification systems do not accurately identify the uncommon causative species of candidiasis, including Candida famata, Meyerozyma guilliermondii, and C. auris. We investigated the accuracy of species-level identification in a collection of clinical isolates previously identified as C. famata (N=38), C. lusitaniae (N=12), and M. guilliermondii (N=5) by the Vitek 2 system. All 55 isolates were re-analyzed by the Phoenix system (Becton Dickinson Diagnostics), two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analyzers (a Vitek MS and a Bruker Biotyper), and by sequencing of internal transcribed space (ITS) regions or 26S rRNA gene D1/D2 domains...
October 6, 2016: Journal of Microbiology and Biotechnology
Maïté Micaelo, Pascal Rasmy, Marlène Amara, Juliette Lambert, Aymeric Coutard, Béatrice Pangon
Erysipelothrix rhusiopathiae, a Gram-positive bacillus, is reported to cause for cutaneous infections and endocarditis. We report a case of E. rhusiopathiae bacteremia without severe clinical illness. The patient, a 74-year-old man, is suffering from a chronic lymphoid leukemia (LLC). Following a trauma, the patient developed a bruise on the left inch. Because the site of shock seemed clinically infected, oral amoxicilline-acid clavulanic (AAC) treatment was started after withdrawn 1 set of blood cultures. These blood culture specimens yielded a Gram-positive bacillus identified as E...
October 1, 2016: Annales de Biologie Clinique
Jung-Yien Chien, Tai-Fen Lee, Shin-Hei Du, Shih-Hua Teng, Chun-Hsing Liao, Wang-Hui Sheng, Lee-Jene Teng, Po-Ren Hsueh
We used an in-house saponin-based extraction method to evaluate the performance of the Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) system for the identification of bacteria and fungi in 405 positively flagged blood culture bottles. Results obtained from MALDI-TOF/MS were compared with those obtained using conventional phenotypic identification methods. Of the 405 positively flagged blood culture bottles, 365 showed monomicrobal growth and were correctly identified to the species (72...
2016: Frontiers in Microbiology
Michael B Jirjis, Chris Valdez, Aditya Vedantam, Brian D Schmit, Shekar N Kurpad
OBJECTIVE The aims of this study were to determine if the morphological and functional changes induced by neural stem cell (NSC) grafts after transplantation into the rodent spinal cord can be detected using MR diffusion tensor imaging (DTI) and, furthermore, if the DTI-derived mean diffusivity (MD) metric could be a biomarker for cell transplantation in spinal cord injury (SCI). METHODS A spinal contusion was produced at the T-8 vertebral level in 40 Sprague Dawley rats that were separated into 4 groups, including a sham group (injury without NSC injection), NSC control group (injury with saline injection), co-injection control group (injury with Prograf), and the experimental group (injury with NSC and Prograf injection)...
September 30, 2016: Journal of Neurosurgery. Spine
Lucas M M Marques, Daniel R Callejon, Larissa G Pinto, Michel L de Campos, Anderson R M de Oliveira, Ricardo Vessecchi, Achyut Adhikari, Ram L S Shrestha, Rosangela G Peccinini, Norberto P Lopes
Govaniadine (GOV) is an alkaloid isolated from Corydalis govaniana Wall. It has been reported to show a different number of biological activities including anti-urease, leishmanicidal and antinociceptive. The present study aims to characterize the GOV in vitro metabolism after incubation with rat and human liver microsomes (RLM and HLM, respectively) and to evaluate its pharmacokinetic properties. The identification of GOV metabolites was conducted by different mass analyzers: a micrOTOF II-ESI-ToF Bruker Daltonics(®) and an amaZon-SL ion trap (IT) Bruker Daltonics(®)...
November 30, 2016: Journal of Pharmaceutical and Biomedical Analysis
Shannon R Sweeney, Arthur Kavanaugh, Alessia Lodi, Bo Wang, David Boyle, Stefano Tiziani, Monica Guma
To determine whether characterisation of patients' metabolic profiles, utilising nuclear magnetic resonance (NMR) and mass spectrometry (MS), could predict response to rituximab therapy. 23 patients with active, seropositive rheumatoid arthritis (RA) on concomitant methotrexate were treated with rituximab. Patients were grouped into responders and non-responders according to the American College of Rheumatology improvement criteria, at a 20% level at 6 months. A Bruker Avance 700 MHz spectrometer and a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer were used to acquire (1)H-NMR and ultra high pressure liquid chromatography (UPLC)-MS/MS spectra, respectively, of serum samples before and after rituximab therapy...
2016: RMD Open
Amanda B Longo, Phil L Salmon, Wendy E Ward
The parameters of a micro-computed tomography (μCT) scan, including whether a bone is imaged in vivo or ex vivo, determine the quality of the resulting image. In turn, this impacts the accuracy of the trabecular and cortical outcomes. The absolute impact of μCT scanning at different voxel sizes and whether the sample is imaged in vivo or ex vivo on the morphological outcomes of the proximal tibia in the rat is unknown. The right proximal tibia of 6-month-old Sham-control and ovariectomized (OVX) rats (n = 8/group) was scanned using μCT (SkyScan 1176, Bruker, Kontich, Belgium) using three sets of parameters (9 μm ex vivo, 18 μm ex vivo, 18 μm in vivo) to compare the trabecular and cortical outcomes...
September 14, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Veronique Deschodt-Arsac, Laurent Arsac, Julie Magat, Jerome Naulin, Bruno Quesson, Pierre Dos Santos
INTRODUCTION: Gradual alterations in cardiac energy balance, as assessed by the myocardial PCr/ATP-ratio, are frequently associated with the development of cardiac disease. Despite great interest for the follow-up of myocardial PCr and ATP content, cardiac MR-spectroscopy in rat models in vivo is challenged by sensitivity issues and cross-contamination from other organs. METHODS: Here we combined MR-Imaging and MR-Spectroscopy (Bruker BioSpec 9.4T) to follow-up for the first time in vivo the cardiac energy balance in the SHR, a genetic rat model of cardiac hypertrophy known to develop early disturbances in cytosolic calcium dynamics...
2016: PloS One
Maud Foissac, Maria Lekaditi, Bouchra Loutfi, Agnès Ehrhart, Frédéric-Antoine Dauchy
INTRODUCTION: Staphylococcus hyicus is a coagulase-variable Staphylococcus spp. well-known by veterinarians since it is the major agent of a severe cutaneous infection in piglets called exudative epidermitis. In other species the symptoms of infection are quite different. Human cases are uncommon but seem to occur more frequently after repeated contacts with farm animals. CASE REPORT: We report the case of a 58-year-old man suffering from debilitating subacute lumbar pain, in whom diagnosis of infectious spondylodiscitis was based on spine MRI and positive microbiological results...
September 2016: Germs
Menglan Zhou, Qiwen Yang, Timothy Kudinha, Li Zhang, Meng Xiao, Fanrong Kong, Yupei Zhao, Ying-Chun Xu
There are challenges in viridans group streptococci (VGS) identification especially for the mitis group. Few studies have investigated the performance of MALDI-TOF MS system in VGS identification. Using 16S rRNA gene and gyrB gene sequencing as a gold standard, the performance of two MALDI-TOF MS instruments in the identification of 181 VGS clinical isolates was studied. The Bruker Biotyper and Vitek MS IVD systems correctly identified 88.4% and 98.9% of the 181 isolates, respectively. The Vitek MS RUO system was the least reliable, only correctly identifying 38...
2016: Frontiers in Microbiology
Sebastian Stach, Ştefan Ţălu, Silvia Trabattoni, Silvia Tavazzi, Alicja Głuchaczka, Patrycja Siek, Joanna Zając, Stefano Giovanzana
PURPOSE: The aim of this study was to quantitatively characterize the micromorphology of contact lens (CL) surfaces using atomic force microscopy (AFM) and multifractal analysis. MATERIALS AND METHODS: AFM and multifractal analysis were used to characterize the topography of new and worn siloxane-hydrogel CLs made of Filcon V (I FDA group). CL surface roughness was studied by AFM in intermittent-contact mode, in air, on square areas of 25 and 100 μm(2), by using a Nanoscope V MultiMode (Bruker)...
September 9, 2016: Current Eye Research
Pietro Zedde, Sebastiano Cudoni, Giacomo Giachetti, Maria Lucia Manunta, Gerolamo Masala, Antonio Brunetti, Andrea Fabio Manunta
PURPOSE: microfracture, providing direct stimulation of chondrogenic mesenchymal stem cells (MSCs) in the subchondral bone, remains the most frequently used primary cartilage repair technique. However, the newly formed type I collagen-rich fibrocartilaginous tissue has poor biomechanical properties and a tendency to degenerate. To overcome these limitations the nanofracture technique was introduced. Our purpose was to compare subchondral bone remodeling 6 months after microfracture versus nanofracture (subchondral needling) treatment in an ovine model...
April 2016: Joints
Eyup Burak Kucuk, Siddik Malkoc, Abdullah Demir
INTRODUCTION: The aim of this study was to use microcomputed tomography to evaluate the effects on white spot lesions of 3 remineralizing agents compared with artificial saliva (Inonu University, Malatya, Turkey). The agents were GC Tooth Mousse (GC International, Itabashi-ku, Tokyo, Japan), 50-ppm sodium fluoride solution (Inonu University, Malatya, Turkey), and Clinpro 5000 (3M ESPE Dental Products (St Paul, Minn). The experimental and control teeth were stored in artificial saliva...
September 2016: American Journal of Orthodontics and Dentofacial Orthopedics
Mikhail Y Ivanov, Vladimir A Nadolinny, Elena G Bagryanskaya, Yuriy A Grishin, Matvey V Fedin, Sergey L Veber
High purity bismuth germanate (Bi4(GeO4)3, BGO) is proposed and implemented as an alternative material for dielectric EPR resonators. A significant improvement of the absolute sensitivity can be readily achieved by substituting the alumina insert (ring) by BGO-made one in commercially available X-band EPR probeheads. Four BGO dielectric inserts of 2, 3, 4 and 5mm inner diameter (ID) were made for comparison with standard 5mm inner diameter alumina insert. All inserts were introduced into commercial Bruker EPR resonator ER 4118X-MD-5W1, and their performance was investigated...
October 2016: Journal of Magnetic Resonance
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Jing-Wei Cheng, Xin-Xin Chen, Zhi-Peng Xu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida haemulonii complex (Candida haemulonii, Candida haemulonii var. vulnera and Candida duobushaemulonii) are emerging pathogens. Thirty-one isolates from 14 hospitals in China were studied for their species classification and antifungal susceptibilities. Molecular (i.e. rDNA internal transcribed spacer (ITS) sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)) and phenotypic identification methods were compared for their performance in species identification. Twenty-six of 31 (83...
August 17, 2016: Journal of Clinical Microbiology
Johannes A Delport, Arend Strikwerda, Amanda Armstrong, David Schaus, Michael John
Sepsis has seen an incremental increase in cases of about 13% annually in the USA and accounts for approximately 4400 deaths among pediatric patients. Early identification of the specific pathogen allows the clinician to ensure that the antibiotic coverage is optimal, an intervention that has been shown to improve patient outcomes in sepsis. Our study's objective was to assess the impact of a rapid Bruker MALDI-Tof identification protocol on pediatric sepsis cases by assessing various indicators. We assessed the quality of care by measuring the following indicators; time to identification of the pathogen, initiation of the most appropriate antibiotic, length of stay (LOS) in hospital and patient outcomes, using a retrospective review over three consecutive years...
2016: PloS One
Jung-Yien Chien, Chong-Jen Yu, Po-Ren Hsueh
AIM: The performance of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying species of nontuberculous mycobacteria (NTM) in flagged Mycobacteria Growth Indicator Tubes (MGIT)s is unclear. MATERIALS & METHODS: A total of 92 sequential MGIT-positive nontuberculous mycobacteria isolates, including Mycobacterium intracellulare, M. abscessus complex, M. avium complex and M. avium, were used to compare the performance of the MALDI-TOF MS for species-level identification with that of the BluePoint MycoID plus kit and final identification...
August 2016: Future Microbiology
Claire Heit, Peter Eriksson, David C Thompson, Georgia Charkoftaki, Kristofer S Fritz, Vasilis Vasiliou
BACKGROUND: There is controversy regarding the active agent responsible for alcohol addiction. The theory that ethanol (EtOH) itself was the agent in alcohol drinking behavior was widely accepted until acetaldehyde (AcH) was found in the brain. The importance of AcH formation in the brain is still subject to speculation due to the lack of a method to accurately assay the AcH levels directly. A highly sensitive gas chromatography mass spectrometry (GC-MS) method to reliably determine AcH concentration with certainty is needed to address whether neural AcH is indeed responsible for increased alcohol consumption...
September 2016: Alcoholism, Clinical and Experimental Research
Eduardo Inocente Jussiani, Paulo José Dos Reis, Carlos Roberto Appoloni
X-ray microtomography is a 3D non-destructive method which, through digital images, enables a view of the internal structure of samples. Recently, researchers have been extensively performing various methods in the attempt to determine the chemical composition of materials. This paper brings further insight into this matter and proposes a new experimental method for determining the internal chemical composition of samples. Using a set of standard samples, calibration curves can be created that allow to relate the average gray scale of a sample obtained through microtomographic images to the value of ρZeff(m)...
October 2016: Micron: the International Research and Review Journal for Microscopy
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