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https://www.readbyqxmd.com/read/28238629/apolipoprotein-a1-rs5070-a-g-polymorphism-with-stroke-subtypes-in-taiwan
#1
Li-Chi Hsu, Tsong-Hai Lee
BACKGROUND: Apolipoprotein A1 (ApoA1) is a structural protein of high-density lipoprotein cholesterol; ApoA1 is involved in lipid and cholesterol metabolism. This study evaluated the association of ApoA1 polymorphism (rs5070) with different stroke subtypes in Taiwanese individuals. METHODS: A total of 2139 cases, including 614 controls and 708 large artery atherosclerosis (LAA), 377 small-vessel occlusion, and 440 hypertensive intracranial hemorrhage cases, were enrolled in this study...
February 23, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28224768/comparison-of-the-bruker-biotyper-and-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-systems-using-a-formic-acid-extraction-method-to-identify-common-and-uncommon-yeast-isolates
#2
Hyun Seung Lee, Jong Hee Shin, Min Ji Choi, Eun Jeong Won, Seung Jung Kee, Soo Hyun Kim, Myung Geun Shin, Soon Pal Suh
BACKGROUND: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and accurate identification of clinical yeast isolates. In-tube formic acid/acetonitrile (FA/ACN) extraction is recommended prior to the analysis with MALDI Biotyper, but the direct on-plate FA extraction is simpler. We compared the Biotyper with the VITEK MS for the identification of various clinically relevant yeast species, focusing on the use of the FA extraction method...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28199097/calibration-strategies-for-ftir-and-other-iris-instruments-for-accurate-%C3%AE-13c-and-%C3%AE-18o-measurements-of-co2-in-air
#3
Edgar Flores, Joele Viallon, Philippe Moussay, David W T Griffith, Robert Ian Wielgosz
This paper describes calibration strategies in laboratory conditions that can be applied to ensure accurate measurements of the isotopic composition of the CO2 in ultra-dry air, expressed as δ13C and δ18O on the VPDB scale, with either FTIR (in this case a Vertex 70V (Bruker)) or an Isotope Ratio Infrared Spectrometer (IRIS) (in this case a Delta Ray (Thermo Fisher Scientific)). In the case of FTIR a novel methodology using only two standards of CO2 in air with different mole fractions but identical isotopic composition was demonstrated to be highly accurate for measurements of δ13C and δ18O with standard uncertainties of 0...
February 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28161160/non-culture-based-identification-of-mastitis-causing-bacteria-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#4
Juliana Regina Barreiro, Juliano Leonel Gonçalves, Patrícia Aparecida Campos Braga, Aline Gerato Dibbern, Marcos Nogueira Eberlin, Marcos Veiga Dos Santos
The purpose of this study was to evaluate the detection limit of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for direct identification, without previous microbiological culture, of bovine mastitis-causing bacteria from milk samples. Milk samples (n = 15) were experimentally contaminated with Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Escherichia coli to have bacterial counts ranging from 10(3) to 10(9) cfu/mL...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28154553/identification-and-antifungal-susceptibility-profiles-of-candida-nivariensis-and-candida-bracarensis-in-a-multi-center-chinese-collection-of-yeasts
#5
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Shu-Ying Yu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida nivariensis and C. bracarensis are two emerging cryptic species within the C. glabrata complex. Thirteen of these isolates from 10 hospitals in China were studied for their species identification and antifungal susceptibilities. Phenotypic and molecular [rDNA ITS sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)] and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS identification methods were compared for their performance in species identification...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28143403/decision-criteria-for-maldi-tof-ms-based-identification-of-filamentous-fungi-using-commercial-and-in-house-reference-databases
#6
Anne-Cécile Normand, Carole Cassagne, Magali Gautier, Pierre Becker, Stéphane Ranque, Marijke Hendrickx, Renaud Piarroux
BACKGROUND: Several Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry protocols, which differ in identification criteria, have been developed for mold and dermatophyte identification. Currently, the most widely used approach is Bruker technology, although no consensus concerning the log(score) threshold has been established. Furthermore, it remains unknown how far increasing the number of spots to compare results might improve identification performance. In this study, we used in-house and Bruker reference databases as well as a panel of 422 isolates belonging to 126 species to test various thresholds...
January 31, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28133904/lifelong-intake-of-flaxseed-or-menhaden-oil-to-provide-varying-n-6-to-n-3-pufa-ratios-modulate-bone-microarchitecture-during-growth-but-not-after-ovx-in-sprague-dawley-rats
#7
Amanda B Longo, Phillip J Sullivan, Sandra J Peters, Paul J LeBlanc, Gregory R Wohl, Wendy E Ward
SCOPE: Skeletal health is a lifelong process impacted by environmental factors, including nutrient intake. The n-3 source and PUFA ratio affect bone health in growing rats, or following ovariectomy (OVX), but no study has investigated the longitudinal effect of PUFA-supplementation throughout these periods of bone development. METHODS AND RESULTS: One month old, Sprague-Dawley rats (n = 98) were randomized to receive 1 of 4 diets from 1 through 6 months of age. Diets were modified from AIN-93G to contain a varying amount and source of n-3 (flaxseed versus menhaden oil) to provide an n-6 to n-3 ratio of 10:1 or 5:1...
January 29, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28128639/evaluation-of-three-bacterial-identification-systems-for-species-identification-of-bacteria-isolated-from-bovine-mastitis-and-bulk-tank-milk-samples
#8
Emily Savage, Shubhada Chothe, Valerie Lintner, Traci Pierre, Tammy Matthews, Subhashinie Kariyawasam, Dawn Miller, Deepanker Tewari, Bhushan Jayarao
A study was conducted to evaluate Sensititre(®) Automated Reading and Incubation System 2x System (ARIS), API(®) (API), and Bruker MALDI-TOF MS (MALDI) bacterial species identification systems using 132 diverse bacterial isolates from bovine milk samples and bulk tank milk received at the Penn State Animal Diagnostic Laboratory. The results were compared with 16S rRNA gene sequence analysis, which served as the reference method for species identification. The ARIS, API, and MALDI identified 0%, 40%, and 33...
January 27, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28127202/tracing-overlapping-biological-signals-in-mid-infrared-using-colonic-tissues-as-a-model-system
#9
Ranjit Kumar Sahu, Ahmad Salman, Shaul Mordechai
AIM: To understand the interference of carbohydrates absorbance in nucleic acids signals during diagnosis of malignancy using Fourier transform infrared (FTIR) spectroscopy. METHODS: We used formalin fixed paraffin embedded colonic tissues to obtain infrared (IR) spectra in the mid IR region using a bruker II IR microscope with a facility for varying the measurement area by varying the aperture available. Following this procedure we could measure different regions of the crypt circles containing different biochemicals...
January 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28125697/usefulness-of-maldi-tof-ms-as-a-diagnostic-tool-for-the-identification-of-streptococcus-species-recovered-from-clinical-specimens-of-pigs
#10
Marta Pérez-Sancho, Ana I Vela, Teresa García-Seco, Sergio González, Lucas Domínguez, Jose Francisco Fernández-Garayzábal
The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S...
2017: PloS One
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#11
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28124778/rapid-maldi-biotyper-based-identification-and-cluster-analysis-of-streptococcus-iniae
#12
Si Won Kim, Seong Won Nho, Se Pyeong Im, Jung Seok Lee, Jae Wook Jung, Jassy Mary S Lazarte, Jaesung Kim, Woo-Jai Lee, Jeong-Ho Lee, Tae Sung Jung
Streptococcus iniae causes severe mortalities among cultured marine species, especially in the olive flounder (Paralichthys olivaceus), which is economically important in Korea and Japan. Recently, there has been growing concern regarding the emergence of S. iniae as a zoonotic pathogen. Here, 89 S. iniae isolates obtained from diseased olive flounders collected from 2003 to 2008 in Jeju Island, South Korea, were characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28119478/rapid-direct-detection-of-carbapenemase-producing-enterobacteriaceae-in-clinical-urine-samples-by-maldi-tof-ms-analysis
#13
Marina Oviaño, Cecilia de la Luna Ramírez, Luis Pedro Barbeyto, Germán Bou
OBJECTIVES: Development of an automated MALDI-TOF MS-based method for the rapid, direct detection of carbapenemase-producing Enterobacteriaceae in clinical urine samples within 90 min of sample reception. METHODS: A total of 3041 urine samples were processed by flow cytometry, and a cut-off value of ≥1.5 × 10(5) bacteria/mL was used to select samples for the study. Following these criteria, 608 samples were selected for direct bacterial identification...
January 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28109550/rapid-detection-of-high-risk-enterococcus-faecium-clones-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#14
Ana R Freitas, Clara Sousa, Carla Novais, Liliana Silva, Helena Ramos, Teresa M Coque, João Lopes, Luísa Peixe
We aimed to explore the potential of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for early identification of dominant Enterococcus faecium (Efm) clones involved in human infections. Well-characterized Efm isolates (n=77), analyzed by pulsed-field gel electrophoresis and multilocus sequence typing(eBURST and BAPS [Bayesian analysis of population structure] algorithms), and belonging to different hospital (n=53) and community (n=24) phylogenomic groups, were tested...
December 14, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28108794/maldi-tof-short-incubation-identification-from-blood-cultures-is-associated-with-reduced-length-of-hospitalization-and-a-decrease-in-bacteremia-associated-mortality
#15
J A Delport, A Strikwerda, A Armstrong, D Schaus, M John
The purpose of this study was to assess the impact of MALDI-ToF identification and rapid short incubation MALDI-Tof identification protocol on patient care compared to conventional identification. By using a retrospective review we assessed the impact of a rapid Bruker MALDI-Tof identification protocol. Overall there was a 16.76-hour reduction in time to identification of the pathogen after the introduction of MALDI-TOF identification in 2013 (P<0.0001) and a further 15-hour reduction (P<9.37 E-05) after implementation of the short incubation MALDI-TOF identification protocol in 2014...
January 20, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28100598/molecular-and-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-characterization-of-clinically-significant-melanized-fungi-in-india
#16
Ashutosh Singh, Pradeep Kumar Singh, Anil Kumar, Jagdish Chander, Geetika Khanna, Pradip Roy, Jacques F Meis, Anuradha Chowdhary
Melanized or black fungi are a heterogeneous group of fungi causing cutaneous to systemic diseases with high mortality. These fungi are rarely reported as agent of human infections primarily due to difficulties in their classical identification. In this study we examined using molecular methods and MALDI-TOF MS the diversity of melanized fungi (MF) isolated from patients in 19 medical centers in India. Overall, during a 4-year period, 718 (5.3%) clinical specimens yielded MF. Of these, 72 (10%) isolates had clinical significance and were identified primarily by sequencing the ITS and LSU regions...
January 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28100314/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-rapid-identification-of-fungal-rhinosinusitis-pathogens
#17
Xinxin Lu, Yanfei Huang, Mingxin Zhang, Min Zhu, Mei Wang, Yufeng Sun, Haitong Gu, Jingjing Cao, Xue Li, Shaoya Zhang, Jinglin Wang
BACKGROUND: filamentous fungi are the most important pathogens causing fungal rhinosinusitis (FRS). Current laboratory diagnosis of FRS pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time-consuming and expertise-dependent. Although matrix -assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed to identify various fungi, its performance for the identification of FRS fungi is less clear...
January 17, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28064071/enhanced-efficiency-of-solid-state-nmr-investigations-of-energy-materials-using-an-external-automatic-tuning-matching-eatm-robot
#18
Oliver Pecher, David M Halat, Jeongjae Lee, Zigeng Liu, Kent J Griffith, Marco Braun, Clare P Grey
We have developed and explored an external automatic tuning/matching (eATM) robot that can be attached to commercial and/or home-built magic angle spinning (MAS) or static nuclear magnetic resonance (NMR) probeheads. Complete synchronization and automation with Bruker and Tecmag spectrometers is ensured via transistor-transistor-logic (TTL) signals. The eATM robot enables an automated "on-the-fly" re-calibration of the radio frequency (rf) carrier frequency, which is beneficial whenever tuning/matching of the resonance circuit is required, e...
December 23, 2016: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28056838/first-case-report-of-myopericarditis-linked-to-campylobacter-coli-enterocolitis
#19
Cameron R M Moffatt, Soniah B Moloi, Karina J Kennedy
BACKGROUND: Campylobacter spp. are a common cause of mostly self-limiting enterocolitis. Although rare, pericarditis and myopericarditis have been increasingly documented as complications following campylobacteriosis. Such cases have occurred predominantly in younger males, and involved a single causative species, namely Campylobacter jejuni. We report the first case of myopericarditis following Campylobacter coli enterocolitis, with illness occurring in an immunocompetent middle-aged female...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28050983/wear-resistance-of-bulk-fill-composite-resin-restorative-materials-polymerized-under-different-curing-intensities
#20
Fahad Alkhudhairy
INTRODUCTION: The aim of this study was to assess the wear resistance of four bulk-fill composite resin restorative materials cured using high- and low-intensity lights. MATERIALS AND METHODS: Twenty-four samples were prepared from each composite resin material (Tetric N-Ceram, SonicFill, Smart Dentin Replacement, Filtek Bulk-Fill) resulting in a total of 96 samples; they were placed into a mold in a single increment. All of the 96 samples were cured using the Bluephase N light curing unit for 20 seconds...
January 1, 2017: Journal of Contemporary Dental Practice
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