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https://www.readbyqxmd.com/read/29228397/yeast-identification-by-sequencing-biochemical-kits-maldi-tof-ms-and-rep-pcr-dna-fingerprinting
#1
Ying Zhao, Chi-Ching Tsang, Meng Xiao, Jasper F W Chan, Susanna K P Lau, Fanrong Kong, Yingchun Xu, Patrick C Y Woo
No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29226548/diffusion-tensor-imaging-determines-three-dimensional-architecture-of-human-cervix-a-cross-sectional-study
#2
J P Nott, E Pervolaraki, A P Benson, E A Bonney, J D Pickering, N Wilkinson, Nab Simpson
OBJECTIVE: To determine the microarchitecture of the cervix using high-resolution diffusion tensor (DT) magnetic resonance imaging (MRI). DESIGN: Cross-sectional study. SETTING: Leeds, UK. SAMPLE: Women undergoing hysterectomy for benign pathology. METHODS: Ex-vivo DT-MRI measurements were obtained using a 9.4-T Bruker nuclear magnetic resonance (NMR) spectrometer on seven fixed human cervices obtained at hysterectomy...
October 26, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29211924/anatolia-nmr-software-for-spectral-analysis-of-total-lineshape
#3
D A Cheshkov, K F Sheberstov, D O Sinitsyn, V A Chertkov
In this paper we present a new fast and free open-source software package ANATOLIA for the total lineshape analysis of NMR spectra. It performs fitting of experimental 1D NMR spectra based on quantum mechanical formalism. The program serves for the determination of J-coupling constants and elucidation of complicated mutliplet structures including strongly coupled systems. The program utilizes the Lorentzian broadening approach for local minima suppression. It is developed in the C++ language for standard personal computers...
December 6, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/29205621/multiparametric-characterization-of-response-to-anti-angiogenic-therapy-using-uspio-contrast-enhanced-mri-in-combination-with-dynamic-contrast-enhanced-mri
#4
Jana Kim, Eugene Kim, Leslie R Euceda, Dan E Meyer, Karina Langseth, Tone F Bathen, Siver A Moestue, Else Marie Huuse
BACKGROUND: Steady state susceptibility contrast (SSC)-MRI provides information on vascular morphology but is a rarely used method. PURPOSE: To investigate the utility of the ultrasmall superparamagnetic iron oxide particles (USPIOs) GEH121333 for measuring tumor response to bevacizumab and compare this with gadolinium-based DCE-MRI. STUDY TYPE: Prospective preclinical animal model study. ANIMAL MODEL: Mice bearing subcutaneous TOV-21G human ovarian cancer xenografts treated with bevacizumab (n = 9) or saline (n = 9)...
December 4, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/29196922/nosocomial-candiduria-in-the-elderly-microbiological-diagnosis
#5
Lidia García-Agudo, Manuel Rodríguez-Iglesias, Rafael Carranza-González
Candiduria is associated with high morbidity, mortality, and long hospitalization, involving high costs for the healthcare system. The use of increasingly aggressive treatments has prolonged the lives of patients susceptible to candiduria, namely the immunosuppressed, the premature, and the elderly. Our objective was to evaluate the incidence of nosocomial candiduria and the implicated species in hospitalized patients aged over 80 years old from three Spanish centers during 2012 and 2013. Urine samples received from these patients were cultured and analyzed by flow cytometry in search of leukocyturia, hematuria, proteinuria, and microbial nitrate reductase activity...
December 1, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29191475/evaluation-of-bruker-biotyper-and-vitek-ms-for-the-identification-of-candida-tropicalis-on-different-solid-culture-media
#6
He Wang, Ying Li, Xin Fan, Tzong-Shi Chiueh, Ying-Chun Xu, Po-Ren Hsueh
BACKGROUND: The aim of this study was to investigate the performance of the Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek MS systems for identification of genetically-confirmed blood isolates of Candida tropicalis that had been grown on several types of culture media commonly used for primary fungal isolation. METHODS: Isolates included 105 from the National China Hospital Invasive Fungal Surveillance Net program (CHIF-NET) and 120 from National Taiwan University Hospital (NTUH)...
November 11, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29183185/the-effect-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-instrumentation-parameters-on-the-matrix-assisted-laser-desorption-ionization-simulated-size-exclusion-chromatography-number-mass-average-weight-and-polydispersity-values
#7
S Bashir, A E Giannakopulos, J Liu
The matrix-assisted laser desorption/ionization simulated size exclusion chromatography (SECPC) average-number mass, weight average and polydispersity of dextran 1000 were determined by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry. The instrument parameters were varied and the SECPC value determined via the Bruker XMASS software was compared to the value obtained from aqueous-phase size exclusion chromatography. The aqueous-phase size exclusion chromatography values for average-number mass, weight average and polydispersity were 1223 Da, 1500 Da and 1...
December 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29175697/in-vivo-study-of-erysolin-metabolic-profile-by-ultra-high-performance-liquid-chromatography-coupleded-to-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#8
Binglong Li, Hui Zhou, Guochun Yang, Fei Han, Yanting Li, Yongfeng Gao, Jinwei Gao, Feng Zhang, Lixin Sun
An ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was developed for the first time to study the in vivo metabolism of erysolin, a compound derived from cruciferous plants which has a definite effect of anti-tumor and anti-nerve injury. In this research, the chromatographic separation was performed on an ACQUITY UPLC® BEH C18 column (2.1 mm×100mm, 1.7μm, Waters, USA) and eluted by a gradient program, the identification work was achieved on a Bruker ultra-high resolution spectrometer in positive ion mode...
November 16, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29175499/visualization-of-porcine-eye-anatomy-by-x-ray-microtomography
#9
Bartosz Leszczyński, Paulina Sojka-Leszczyńska, Dorota Wojtysiak, Andrzej Wróbel, Roman Pędrys
The aim of our study is to obtain, as accurately as possible, porcine ocular tissue visualization using microtomography (micro-CT) method. We propose image contrast enhancement by different staining procedures with combination of micro-CT scanning. Porcine eye globes were investigated with Bruker-SkyScan 1172 micro-CT. We used 4F1G and Bouin's as sample fixation solutions and tincture of iodine, 100% Lugol, phosphotungstic acid and 1% osmium tetroxide solutions for staining. Quantitative and qualitative analysis was performed based on micro-CT reconstruction images histograms and 3D volume rendering models of investigated samples...
November 22, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29153581/novel-application-of-micro-computerized-tomography-for-morphologic-characterization-of-the-murine-penis
#10
Marisol O'Neill, Gene O Huang, Dolores J Lamb
BACKGROUND: The murine penis model has enriched our understanding of anomalous penile development. The morphologic characterization of the murine penis using conventional serial sectioning methods is labor intensive and prone to errors. AIM: To develop a novel application of micro-computerized tomography (micro-CT) with iodine staining for rapid, non-destructive morphologic study of murine penis structure. METHODS: Penises were dissected from 10 adult wild-type mice and imaged using micro-CT with iodine staining...
November 16, 2017: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/29153517/rapid-species-and-subspecies-specific-level-classification-and-identification-of-lactobacillus-casei-group-members-using-maldi-biotyper-combined-with-clinprotools
#11
Chien-Hsun Huang, Lina Huang
Using common taxonomic methods such as 16S rDNA sequencing and physiological and biochemical analysis to identify members of the Lactobacillus casei group (LCG) is time-consuming, expensive and inaccurate. In this study, we applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapid discriminate LCG strains by creating an analytical in-house database (IHDB) and to develop a classification model for subspecies-level differentiation based on MS biomarkers using ClinProTools bioinformatics software (Bruker Daltonics, Billerica, MA)...
November 15, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29136819/alteration-in-the-liver-metabolome-of-rats-with-metabolic-syndrome-after-treatment-with-hydroxytyrosol-a-mass-spectrometry-and-nuclear-magnetic-resonance-based-metabolomics-study
#12
Ioanna Dagla, Dimitra Benaki, Eirini Baira, Nikolaos Lemonakis, Hemant Poudyal, Lindsay Brown, Anthony Tsarbopoulos, Alexios-Leandros Skaltsounis, Emmanouel Mikros, Evagelos Gikas
Metabolic syndrome (MetS) represents a group of abnormalities that enhances the risk for cardiovascular disease, diabetes and stroke. The Mediterranean diet seems to be an important dietary pattern, which reduces the incidence of MetS. Hydroxytyrosol (HT) - a simple phenol found in olive oil - has received increased attention for its antioxidant activity. Recently, the European Foods Safety Authority (EFSA) claimed that dietary consumption of HT exhibits a protective role against cardiovascular disease. In this study, an experimental protocol has been setup, including isolated HT administration in a diet induced model of MetS in young Wistar rats, in order to find out whether HT has a protective effect against MetS...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29131518/metabolic-profile-of-kudiezi-injection-in-rats-by-uhplc-coupled-with-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#13
Jingdan Zhang, Xiaoxue Zhang, Yangyang Zhao, Aihua Song, Wei Sun, Ran Yin
In this study, a reliable and sensitive ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry method was developed for the systematic study of the metabolic profile of Kudiezi injection in rat plasma, bile, urine and feces after intravenous administration of a single dose. The chromatographic separation was performed on an Agilent Eclipse Plus C18 column (4.6 mm × 50 mm, 1.8 μm) and the identification of prototype components and metabolites was achieved on a Bruker Solarix 7...
November 13, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29118171/total-laboratory-automation-and-maldi-tof-improve-turnaround-times-in-the-clinical-microbiology-laboratory-a-retrospective-analysis
#14
Talent Theparee, Sanchita Das, Richard B Thomson
Technological advances have changed the practice of clinical microbiology. We implemented Bruker MALDI-TOF and BD Kiestra Total Laboratory Automation (TLA) three and four years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TAT) for positive and negative urine cultures before and after implementation. Comparison I: TATs for 61,157 urine cultures were extracted for two periods corresponding to pre-TLA and post-TLA, both using MALDI-TOF for organism identification...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29110928/direct-bacterial-identification-from-positive-blood-cultures-using-matrix-assisted-laser-desorption-ionization-time-of-flight-maldi-tof-mass-spectrometry-a-systematic-review-and-meta-analysis
#15
Jesús Ruiz-Aragón, Mónica Ballestero-Téllez, Belén Gutiérrez-Gutiérrez, Marina de Cueto, Jesús Rodríguez-Baño, Álvaro Pascual
INTRODUCTION: The rapid identification of bacteraemia-causing pathogens could assist clinicians in the timely prescription of targeted therapy, thereby reducing the morbidity and mortality of this infection. In recent years, numerous techniques that rapidly and directly identify positive blood cultures have been marketed, with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) being one of the most commonly used. METHODS: The aim of this systematic review and meta-analysis was to evaluate the accuracy of MALDI-TOF (Bruker(®)) for the direct identification of positive blood culture bottles...
October 27, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29105210/in-vivo-detection-and-automatic-analysis-of-gaba-in-the-mouse-brain-with-mega-press-at-9-4%C3%A2-t
#16
Jia Guo, Zhu Gang, Yanping Sun, Andrew Laine, Scott A Small, Douglas L Rothman
The goals of this study were to develop an acquisition protocol and the analysis tools for Meshcher-Garwood point-resolved spectroscopy (MEGA-PRESS) in mouse brain at 9.4 T, to allow the in vivo detection of γ-aminobutyric acid (GABA) and to examine whether isoflurane alters GABA levels in the thalamus during anesthesia. We implemented the MEGA-PRESS sequence on a Bruker 94/20 system with ParaVision 6.0.1, and magnetic resonance spectra were acquired from nine male wild-type C57BL/6 J mice at the thalamus...
November 3, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/29073795/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-protocol-standardization-and-database-expansion-for-rapid-identification-of-clinically-important-molds
#17
Saikat Paul, Pankaj Singh, Shivaprakash M Rudramurthy, Arunaloke Chakrabarti, Anup K Ghosh
AIM: To standardize the matrix-assisted laser desorption ionization-time of flight mass spectrometry protocols and expansion of existing Bruker Biotyper database for mold identification. MATERIALS & METHODS: Four different sample preparation methods (protocol A, B, C and D) were evaluated. RESULTS: On analyzing each protein extraction method, reliable identification and best log scores were achieved through protocol D. The same protocol was used to identify 153 clinical isolates...
October 27, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29062198/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-as-a-reliable-proteomic-method-for-characterization-of-escherichia-coli-and-salmonella-isolates
#18
Waleed S Shell, Mahmoud Lotfy Sayed, Fatma Mohamed Gad Allah, Fatma Elzahraa Mohamed Gamal, Afaf Ahmed Khder, A A Samy, Abdel Hakam M Ali
AIM: Identification of pathogenic clinical bacterial isolates is mainly dependent on phenotypic and genotypic characteristics of the microorganisms. These conventional methods are costive, time-consuming, and need special skills and training. An alternative, mass spectral (proteomics) analysis method for identification of clinical bacterial isolates has been recognized as a rapid, reliable, and economical method for identification. This study was aimed to evaluate and compare the performance, sensitivity and reliability of traditional bacteriology, phenotypic methods and matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS) in the identification of clinical Escherichia coli and Salmonella isolates recovered from chickens...
September 2017: Veterinary World
https://www.readbyqxmd.com/read/29061514/bold-mri-demonstrates-acute-placental-and-fetal-organ-hypoperfusion-with-fetal-brain-sparing-during-hypercapnia
#19
Yehuda Ginosar, Yuval Gielchinsky, Nathalie Nachmansson, Lital Hagai, Joel Shapiro, Uriel Elchalal, Rinat Abramovitch
INTRODUCTION: We evaluated changes in placental and fetal hemodynamics in rodents during acute hypercapnia using BOLD-MRI and Doppler ultrasound. METHODS: Animals were anesthetized with pentobarbital and, in consecutive 4-min periods, breathed: air, 21%O2:5%CO2, and 95%O2:5%CO2. BOLD-MRI: Pregnant ICR mice (n = 6; E17.5) were scanned in a 4.7-T Bruker Biospec spectrometer. Placenta and fetal liver, heart and brain were identified on True-FISP images...
September 11, 2017: Placenta
https://www.readbyqxmd.com/read/29055059/application-of-the-bbcid-mass-spectrometry-approach-for-protein-glycosylation-and-phosphorylation-analysis
#20
Narciso Couto, Liliya Davlyatova, Caroline A Evans, Phillip C Wright
RATIONALE: Post-translational modified peptides analysis by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA). An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity. The use of broad band collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation...
October 21, 2017: Rapid Communications in Mass Spectrometry: RCM
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