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S. pombe

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https://www.readbyqxmd.com/read/27906621/isolation-of-a-novel-protein-p12-from-adult-drosophila-melanogaster-that-inhibits-deoxyribonucleoside-and-protein-kinase-activities-and-activates-3-5-exonuclease-activity
#1
Louise Slot Christiansen, Gabriella van Zanten, Dvora Berenstein, Marianne Lauridsen, Søren Kjærulff, Leif Søndergaard, Birgitte Munch-Petersen
We have previously found that Drosophila melanogaster only has one deoxyribonucleoside kinase, Dm-dNK, however, capable to phosphorylate all four natural deoxyribonucleosides. Dm-dNK was originally isolated from an embryonic cell line. We wanted to study the expression of Dm-dNK during development from embryonic cells to adult flies and found declining Dm-dNK activity during development and no activity in adult flies. Surprisingly, the extract from adult flies exhibited a strong inhibitory effect on deoxyribonucloside kinase activity...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27902423/characterization-of-cis-elements-in-the-promoter-of-trz2-encoding-schizosaccharomyces-pombe-mitochondrial-trna-3-end-processing-enzyme
#2
Jinyu Liu, Linting Huang, Yirong Wang, Ying Huang
The endonuclease tRNase Z is responsible for the 3'-end processing of tRNA precursors, which is one of the essential steps in tRNA maturation. The fission yeast Schizosaccharomyces pombe contains two essential tRNase ZL genes (trz1 and trz2) involved in nuclear and mitochondrial tRNA 3'-end processing, respectively. Our previous studies suggest that trz2 is expressed at a very low level. Here we report characterization of the trz2 promoter. Using lacZ as a reporter, we show that the trz2 promoter contains a HomolD box and a very weak diverged TATA element...
November 22, 2016: Microbiology
https://www.readbyqxmd.com/read/27896529/acetaldehyde-kinetics-of-enological-yeast-during-alcoholic-fermentation-in-grape-must
#3
Erhu Li, Ramón Mira de Orduña
Acetaldehyde strongly binds to the wine preservative SO2 and, on average, causes 50-70 mg l(-1) of bound SO2 in red and white wines, respectively. Therefore, a reduction of bound and total SO2 concentrations necessitates knowledge of the factors that affect final acetaldehyde concentrations in wines. This study provides a comprehensive analysis of the acetaldehyde production and degradation kinetics of 26 yeast strains of oenological relevance during alcoholic fermentation in must under controlled anaerobic conditions...
November 28, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27890612/in%C3%A2-vitro-reconstitution-and-biochemical-analyses-of-the-schizosaccharomyces-pombe-nucleosome
#4
Masako Koyama, Wataru Nagakura, Hiroki Tanaka, Tomoya Kujirai, Yuji Chikashige, Tokuko Haraguchi, Yasushi Hiraoka, Hitoshi Kurumizaka
Schizosaccharomyces pombe, which has a small genome but shares many physiological functions with higher eukaryotes, is a useful single-cell, model eukaryotic organism. In particular, many features concerning chromatin structure and dynamics, including heterochromatin, centromeres, telomeres, and DNA replication origins, are well conserved between S. pombe and higher eukaryotes. However, the S. pombe nucleosome, the fundamental structural unit of chromatin, has not been reconstituted in vitro. In the present study, we established the method to purify S...
November 24, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27886462/loss-of-ppr3-ppr4-ppr6-or-ppr10-perturbs-iron-homeostasis-and-leads-to-apoptotic-cell-death-in-schizosaccharomyces-pombe
#5
Yang Su, Yanmei Yang, Ying Huang
Pentatricopeptide repeat (PPR) proteins characterized by tandem arrays of a degenerate 35-amino-acid repeat belong to a large family of RNA-binding proteins that are involved in posttranscriptional control of organelle gene expression. PPR proteins are ubiquitous in eukaryotes, and particularly prevalent in higher plants. Schizosaccharomyces pombe has ten PPR proteins. Among them, ppr3, ppr4, ppr6 and ppr10 participate in mitochondrial posttranscriptional processes and are required for mitochondrial electron transport chain (ETC) function...
November 25, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27884105/structural-features-of-dna-that-determine-rna-polymerase-ii-core-promoter
#6
Irina A Il'icheva, Mingian V Khodikov, Maria S Poptsova, Dmitry Yu Nechipurenko, Yury D Nechipurenko, Sergei L Grokhovsky
BACKGROUND: The general structure and action of all eukaryotic and archaeal RNA polymerases machinery have an astonishing similarity despite the diversity of core promoter sequences in different species. The goal of our work is to find common characteristics of DNA region that define it as a promoter for the RNA polymerase II (Pol II). RESULTS: The profiles of a large number of physical and structural characteristics, averaged over representative sets of the Pol II minimal core promoters of the evolutionary divergent species from animals, plants and unicellular fungi were analysed...
November 25, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27875300/the-fission-yeast-pre-mrna-processing-factor-18-prp18-has-intron-specific-splicing-functions-with-links-to-g1-s-cell-cycle-progression
#7
Nagampalli Vijaykrishna, Geetha Melangath, Rakesh Kumar, Piyush Khandelia, Pushpinder Bawa, Raghavan Varadarajan, Usha Vijayraghavan
The fission yeast genome which contains numerous short introns is an apt model for studies on fungal splicing mechanisms and splicing by intron-definition. Here we perform a domain analysis of the evolutionarily conserved Schizosaccharomyces pombe pre-mRNA processing factor, SpPrp18. Our mutational and biophysical analyses of the C- terminal alpha-helical bundle reveal critical roles for the conserved region as well as and helix five. We generate a novel conditional missense mutant, spprp18-5 To assess the role of SpPrp18, we performed global splicing analyses on cells depleted of prp18+ and the conditional spprp18-5 mutant which show widespread but intron-specific defects...
November 15, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27844442/quantifying-force-and-viscoelasticity-inside-living-cells-using-an-active-passive-calibrated-optical-trap
#8
Christine M Ritter, Josep Mas, Lene Oddershede, Kirstine Berg-Sørensen
As described in the previous chapters, optical tweezers have become a tool of precision for in vitro single-molecule investigations, where the single molecule of interest most often is studied in purified form in an experimental assay with a well-controlled fluidic environment. A well-controlled fluidic environment implies that the physical properties of the liquid, most notably the viscosity, are known and the fluidic environment can, for calibrational purposes, be treated as a simple liquid.In vivo, however, optical tweezers have primarily been used as a tool of manipulation and not so often for precise quantitative force measurements, due to the unknown value of the spring constant of the optical trap formed within the cell's viscoelastic cytoplasm...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27837315/cloning-and-characterization-of-decaprenyl-diphosphate-synthase-from-three-different-fungi
#9
Daisuke Moriyama, Tomohiro Kaino, Kazuyoshi Yajima, Ryota Yanai, Yasuhiro Ikenaka, Junzo Hasegawa, Motohisa Washida, Hirokazu Nanba, Makoto Kawamukai
Coenzyme Q (CoQ) is composed of a benzoquinone moiety and an isoprenoid side chain of varying lengths. The length of the side chain is controlled by polyprenyl diphosphate synthase. In this study, dps1 genes encoding decaprenyl diphosphate synthase were cloned from three fungi: Bulleromyces albus, Saitoella complicata, and Rhodotorula minuta. The predicted Dps1 proteins contained seven conserved domains found in typical polyprenyl diphosphate synthases and were 528, 440, and 537 amino acids in length in B. albus, S...
November 11, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27825301/analysis-of-the-association-between-codon-optimality-and-mrna-stability-in-schizosaccharomyces-pombe
#10
Yuriko Harigaya, Roy Parker
BACKGROUND: Recent experiments have shown that codon optimality is a major determinant of mRNA stability in Saccharomyces cerevisiae and that this phenomenon may be conserved in Escherichia coli and some metazoans, although work in Neurospora crassa is not consistent with this model. RESULTS: We examined the association between codon optimality and mRNA stability in the fission yeast Schizosaccharomyces pombe. Our analysis revealed the following points. First, we observe a genome-wide association between codon optimality and mRNA stability also in S...
November 8, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27803257/chromatin-immunoprecipitation-chip-in-schizosaccharomyces-pombe
#11
Hugh P Cam, Simon Whitehall
Chromatin immunoprecipitation (ChIP), the cross-linking of chromatin followed by immunoprecipitation with antibodies against a chromatin target, is a key method for measuring association of proteins with a specific genomic region(s). As a negative control, a mock ChIP experiment in which no antibody is added to the immunoprecipitation reaction is included. Enriched DNA fragments from a ChIP experiment can be analyzed in a variety of ways. For semiquantitative analysis, a region of interest can be amplified using standard polymerase chain reaction (PCR) techniques...
November 1, 2016: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/27798241/an-acetyltransferase-independent-function-of-eso1-regulates-centromere-cohesion
#12
Su-Jiun Lin, Claudia Tapia-Alveal, Omar J Jabado, Doris Germain, Matthew J O'Connell
Eukaryotes contain three essential Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6. Cohesin forms a ring-shaped structure that embraces sister chromatids to promote their cohesion. The cohesiveness of cohesin is promoted by acetylation of N-terminal lysines of the Smc3 subunit by the acetyltransferases Eco1 in Saccharomyces cerevisiae, and by the homolog Eso1 in Schizosaccharomyces pombe In both yeasts, these acetyltransferases are essential for cell viability. However, while non-acetylatable Smc3 mutants are lethal in S...
October 19, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27766670/spatial-organization-of-the-schizosaccharomyces-pombe-genome-within-the-nucleus
#13
Atsushi Matsuda, Haruhiko Asakawa, Tokuko Haraguchi, Yasushi Hiraoka
The fission yeast Schizosaccharomyces pombe is a useful experimental system for studying the organization of chromosomes within the cell nucleus. S. pombe has a small genome that is organized into three chromosomes. The small size of the genome and the small number of chromosomes are advantageous for cytological and genome-wide studies of chromosomes; however, the small size of the nucleus impedes microscopic observations owing to limits in spatial resolution during imaging. Recent advances in microscopy, such as super-resolution microscopy, have greatly expanded the use of S...
October 21, 2016: Yeast
https://www.readbyqxmd.com/read/27736907/scarless-gene-tagging-with-one-step-transformation-and-two-step-selection-in-saccharomyces-cerevisiae-and-schizosaccharomyces-pombe
#14
Dirk Landgraf, Dann Huh, Erinc Hallacli, Susan Lindquist
Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of the gene of interest without disrupting regulatory sequences including the 5' and 3' untranslated region (UTR) and without introducing any extraneous unwanted "scar" sequences, which may create unpredictable transcriptional or translational effects. We present a reliable, low-cost, and highly efficient method for the construction of such scarless C-terminal and N-terminal fusions with fluorescent proteins in yeast...
2016: PloS One
https://www.readbyqxmd.com/read/27734801/curvature-induced-expulsion-of-actomyosin-bundles-during-cytokinetic-ring-contraction
#15
Junqi Huang, Ting Gang Chew, Ying Gu, Saravanan Palani, Anton Kamnev, Douglas S Martin, Nicholas J Carter, Robert Anthony Cross, Snezhana Oliferenko, Mohan K Balasubramanian
Many eukaryotes assemble a ring-shaped actomyosin network that contracts to drive cytokinesis. Unlike actomyosin in sarcomeres, which cycles through contraction and relaxation, the cytokinetic ring disassembles during contraction through an unknown mechanism. Here we find in Schizosaccharomyces japonicus and Schizosaccharomyces pombe that, during actomyosin ring contraction, actin filaments associated with actomyosin rings are expelled as micron-scale bundles containing multiple actomyosin ring proteins. Using functional isolated actomyosin rings we show that expulsion of actin bundles does not require continuous presence of cytoplasm...
October 13, 2016: ELife
https://www.readbyqxmd.com/read/27697865/the-inositol-pyrophosphate-kinase-asp1-modulates-chromosome-segregation-fidelity-and-spindle-function-in-s-pombe
#16
Boris Topolski, Visnja Jakopec, Natascha A Künzel, Ursula Fleig
Chromosome transmission fidelity during mitosis is of critical importance for the fitness of an organism as mistakes will lead to aneuploidy, which has a causative role in numerous severe diseases. Proper segregation of chromosomes depends on interdependent processes at the microtubule-kinetochore interface and the spindle assembly checkpoint. Here we report the discovery of a new element essential for chromosome transmission fidelity that implicates inositol pyrophosphates (IPPs) as playing a key role in this process...
October 3, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27697163/cell-wall-polysaccharides-released-during-the-alcoholic-fermentation-by-schizosaccharomyces-pombe-and-s-%C3%A2-japonicus-quantification-and-characterization
#17
P Domizio, Y Liu, L F Bisson, D Barile
The present work demonstrates that yeasts belonging to the Schizosaccharomyces genus release a high quantity of polysaccharides of cell wall origin starting from the onset of the alcoholic fermentation. By the end of the alcoholic fermentation, all of the Schizosaccharomyces yeast strains released a quantity of polysaccharides approximately 3-7 times higher than that released by a commercial Saccharomyces cerevisiae yeast strain under the same fermentative conditions of synthetic juice. A higher content of polysaccharide was found in media fermented by Schizosaccharomyces japonicus with respect to that of Schizosaccharomyces pombe...
February 2017: Food Microbiology
https://www.readbyqxmd.com/read/27696877/engineering-an-abc-transporter-for-enhancing-resistance-to-caffeine-in-saccharomyces-cerevisiae
#18
Min Wang, Wei-Wei Deng, Zheng-Zhu Zhang, Oliver Yu
In addressing caffeine toxicity to the producing cells, engineering a transporter that can move caffeine from cytoplasm across cell membrane to extracellular space, thus enhancing caffeine resistance and potentially increasing the yield in yeast are important. An ABC-transporter bfr1 from Schizosaccharomyces pombe was cloned and transformed into S. cerevisiae, resulting in enhancing caffeine resistance. Afterwards, a library of randomly mutagenized bfr1 mutants through error-prone PCR was generated. It was identified one mutant with drastically increased caffeine resistance (15 mg/mL)...
October 4, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27687771/csl-protein-regulates-transcription-of-genes-required-to-prevent-catastrophic-mitosis-in-fission-yeast
#19
Martin Převorovský, Martina Oravcová, Róbert Zach, Anna Jordáková, Jürg Bähler, František Půta, Petr Folk
For every eukaryotic cell to grow and divide, intricately coordinated action of numerous proteins is required to ensure proper cell-cycle progression. The fission yeast Schizosaccharomyces pombe has been instrumental in elucidating the fundamental principles of cell-cycle control. Mutations in S. pombe 'cut' (cell untimely torn) genes cause failed coordination between cell and nuclear division, resulting in catastrophic mitosis. Deletion of cbf11, a fission yeast CSL transcription factor gene, triggers a 'cut' phenotype, but the precise role of Cbf11 in promoting mitotic fidelity is not known...
September 29, 2016: Cell Cycle
https://www.readbyqxmd.com/read/27678009/expression-pattern-and-phenotypic-characterization-of-the-mutant-strain-reveals-target-genes-and-processes-regulated-by-pka1-in-the-dimorphic-fission-yeast-schizosaccharomyces-japonicus
#20
László Papp, Matthias Sipiczki, Ida Miklós
The cAMP cascade plays an important role in several biological processes. Thus, study of its molecular details can contribute to a better understanding of these processes, treatment of diseases, or even finding antifungal drug targets. To gain further information about the PKA pathway, and its evolutionarily conserved and species-specific features, the central regulator pka1 gene, which encodes the cAMP-dependent protein kinase catalytic subunit, was studied in the less known haplontic, dimorphic fission yeast Schizosaccharomyces japonicus...
September 27, 2016: Current Genetics
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