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https://www.readbyqxmd.com/read/28528978/multimodal-control-of-transcription-factor-pap1-in-schizosaccharomyces-pombe-under-nitrosative-stress
#1
Puranjoy Kar, Pranjal Biswas, Sanjay Ghosh
Schizosaccharomyces pombe Pap1, a bZIP transcription factor, is highly homologous to the mammalian c-Jun protein that belongs to the AP1 family of transcriptional regulators. The role of transcription factor Pap1 has been extensively studied under oxidative stress. Two cysteine residues in Pap1p namely, C278 and C501 form disulfide linkage under oxidative stress resulting in nuclear accumulation. We first time showed the involvement of Pap1 in the protection against nitrosative stress. In the present study we show that pap1 deletion makes growth of S...
May 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28522285/production-of-3-hydroxypropionic-acid-via-the-malonyl-coa-pathway-using-recombinant-fission-yeast-strains
#2
Akiko Suyama, Yujiro Higuchi, Masahiro Urushihara, Yuka Maeda, Kaoru Takegawa
3-Hydroxypropionic acid (3-HP) can be converted into derivatives such as acrylic acid, a source for producing super absorbent polymers. Although Escherichia coli has often been used for 3-HP production, it exhibits low tolerance to 3-HP. To circumvent this problem, we selected the fission yeast Schizosaccharomyces pombe as this microorganism has higher tolerance to 3-HP than E. coli. Therefore, we constructed S. pombe transformants overexpressing two genes, one encoding the S. pombe acetyl-CoA carboxylase (Cut6p) and the other encoding the malonyl-CoA reductase derived from Chloroflexus aurantiacus (CaMCR)...
May 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28494091/fermentation-characteristics-and-aromatic-profile-of-plum-wines-produced-with-indigenous-microbiota-and-pure-cultures-of-selected-yeast
#3
Uroš Miljić, Vladimir Puškaš, Vesna Vučurović, Ana Muzalevski
The aim of this study was to assess and compare fermentation characteristics and aromatic profile of plum wines produced with indigenous microbiota and pure cultures of different selected yeast. Experiments were carried out with plum (Prunus domestica L.) varieties of different fruit ripening times (Čačanska rana, Čačanska lepotica, and Požegača). Wine fermentations were conducted by the activity of indigenous microbiota, commercially available Saccharomyces cerevisiae and Saccharomyces bayanus yeast strains and joint activity of Schizosaccharomyces pombe and S...
May 11, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28481910/pcna-ubiquitylation-ensures-timely-completion-of-unperturbed-dna-replication-in-fission-yeast
#4
Yasukazu Daigaku, Thomas J Etheridge, Yuka Nakazawa, Mayumi Nakayama, Adam T Watson, Izumi Miyabe, Tomoo Ogi, Mark A Osborne, Antony M Carr
PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase δ with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling...
May 8, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28479325/size-dependent-expression-of-the-mitotic-activator-cdc25-suggests-a-mechanism-of-size-control-in-fission-yeast
#5
Daniel Keifenheim, Xi-Ming Sun, Edridge D'Souza, Makoto J Ohira, Mira Magner, Michael B Mayhew, Samuel Marguerat, Nicholas Rhind
Proper cell size is essential for cellular function. Nonetheless, despite more than 100 years of work on the subject, the mechanisms that maintain cell-size homeostasis are largely mysterious [1]. Cells in growing populations maintain cell size within a narrow range by coordinating growth and division. Bacterial and eukaryotic cells both demonstrate homeostatic size control, which maintains population-level variation in cell size within a certain range and returns the population average to that range if it is perturbed [1, 2]...
May 22, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28476936/genome-wide-screen-for-cell-growth-regulators-in-fission-yeast
#6
Louise Weston, Jessica Greenwood, Paul Nurse
Cellular growth control is important for all living organisms but experimental investigation into this problem is difficult because of the complex range of growth regulatory mechanisms. We have used the fission yeast Schizosaccharomyces pombe to identify potential master regulators of growth. The S. pombe pat1(ts)mei4Δ strain enters the meiotic developmental program at restrictive temperature, but arrests in meiotic G2 phase as mei4(+) is essential for meiotic progression. These cells do not grow, even in an abundance of nutrients...
May 5, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28432181/dual-nature-of-pseudouridylation-in-u2-snrna-pus1p-dependent-and-pus1p-independent-activities-in-yeasts-and-higher-eukaryotes
#7
Svetlana Deryusheva, Joseph G Gall
The pseudouridine at position 43 in vertebrate U2 snRNA is one of the most conserved posttranscriptional modifications of spliceosomal snRNAs; the equivalent position is pseudouridylated in U2 snRNAs in different phyla including fungi, insects, and worms. Pseudouridine synthase Pus1p acts alone on U2 snRNA to form this pseudouridine in yeast Saccharomyces cerevisiae and mouse. Furthermore, in S. cerevisiae Pus1p is the only pseudouridine synthase for this position. Using an in vivo yeast cell system we tested enzymatic activity of Pus1p from the fission yeast Schizosaccharomyces pombe, the worm Caenorhabditis elegans, the fruit fly Drosophila melanogaster and the frog Xenopus tropicalis...
April 21, 2017: RNA
https://www.readbyqxmd.com/read/28426144/a-multiplex-culture-system-for-the-long-term-growth-of-fission-yeast-cells
#8
Celine Callens, Nelson C Coelho, Aaron W Miller, Maria Rosa Domino Sananes, Maitreya J Dunham, Matthieu Denoual, Damien Coudreuse
Maintenance of long-term cultures of yeast cells is central to a broad range of investigations, from metabolic studies to laboratory evolution assays. However, repeated dilutions of batch cultures lead to variations in medium composition, with implications for cell physiology. In S. cerevisiae, powerful miniaturized chemostat setups, or ministat arrays, have been shown to allow for constant dilution of multiple independent cultures. Here we set out to adapt these arrays for continuous culture of a morphologically and physiologically distinct yeast, the fission yeast Schizosaccharomyces pombe, with the goal of maintaining constant population density over time...
April 20, 2017: Yeast
https://www.readbyqxmd.com/read/28423198/an-estradiol-inducible-promoter-enables-fast-graduated-control-of-gene-expression-in-fission-yeast
#9
Makoto J Ohira, David G Hendrickson, R Scott McIsaac, Nicholas Rhind
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S...
April 19, 2017: Yeast
https://www.readbyqxmd.com/read/28382430/dna-topoisomerase-ii-modulates-acetyl-regulation-of-cohesin-mediated-chromosome-dynamics
#10
Su-Jiun Lin, Matthew J O'Connell
Cohesin is one of three multi-protein structural maintenance of chromosome (SMC) complexes that regulate eukaryotic chromosome dynamics. It forms a ring-shaped structure that embraces sister chromatids through interphase to promote their pairing. In preparation for mitosis, most cohesin is stripped from the chromosome arms in prophase by a poorly defined process that is associated with cohesin phosphorylation. In the fission yeast Schizosaccharomyces pombe this prophase pathway is dependent on the cohesin-related Smc5/6 complex, and this requirement is heightened in Smc5/6 hypomorphs by DNA damage, replication stress and Topoisomerase II (Top2) dysfunction...
April 5, 2017: Current Genetics
https://www.readbyqxmd.com/read/28369658/tracking-the-evolution-of-3d-gene-organization-demonstrates-its-connection-to-phenotypic-divergence
#11
Alon Diament, Tamir Tuller
It has recently been shown that the organization of genes in eukaryotic genomes, and specifically in 3D, is strongly related to gene expression and function and partially conserved between organisms. However, previous studies of 3D genomic organization analyzed each organism independently from others. Here, we propose an approach for unified inter-organismal analysis of gene organization based on a network representation of Hi-C data. We define and detect four classes of spatially co-evolving orthologous modules (SCOMs), i...
May 5, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28357387/s-pombe-placed-on-the-prion-map
#12
COMMENT
Jacqueline Hayles
Schizosaccharomyces pombe has been used extensively as a model organism, however it is only recently that the first prion in this organism, a copper transporter protein encoded by ctr4, has been conclusively demonstrated. Prions are found in a wide range of organisms and have been implicated in a number of human neurodegenerative diseases. Research into the biology of prions has been carried out mainly in the budding yeast Saccharomyces cerevisiae, however there are many questions still to be addressed. Now, with the identification of the Ctr4 prion in S...
February 3, 2017: Microbial Cell
https://www.readbyqxmd.com/read/28349389/quantitative-genome-wide-measurements-of-meiotic-dna-double-strand-breaks-and-protein-binding-in-s-pombe
#13
Randy W Hyppa, Kyle R Fowler, Gerald R Smith
The fission yeast Schizosaccharomyces pombe is especially well suited for studying meiosis in molecular detail. Experiments with S. pombe strains that undergo a nearly synchronous meiosis-at variable temperatures-have elucidated the mechanisms of meiotic progression and the proteins that are involved. For example, studies focused on the initiation of meiotic recombination by programmed DNA double-strand breaks (DSBs) have proven exceptionally informative. In meiosis, some regions of DNA have more frequent DSBs than the surrounding regions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28345447/urea-enhances-cell-lysis-of-schizosaccharomyces-pombe-ura4-mutants
#14
Kohei Nishino, Misaki Kushima, Tomohiro Kaino, Yasuhiro Matsuo, Makoto Kawamukai
Cell lysis is induced in Schizosaccharomyces pombe ∆ura4 cells grown in YPD medium, which contains yeast extract, polypeptone, and glucose. To identify the medium components that induce cell lysis, we first tested various kinds of yeast extracts from different suppliers. Cell lysis of ∆ura4 cells on YE medium was observed when yeast extracts from OXOID, BD, Oriental, and Difco were used, but not when using yeast extract from Kyokuto. To determine which compounds induced cell lysis, we subjected yeast extract and polypeptone to GC-MS analysis...
March 27, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28342076/schizosaccharomyces-pombe-rsv1-transcription-factor-and-its-putative-homologues-preserved-their-functional-homology-and-are-evolutionarily-conserved
#15
Emese Pataki, Matthias Sipiczki, Ida Miklos
Environmental glucose is an important regulator of biological processes, as it can launch different cell processes depending on its concentration. Thus, low glucose concentration can induce entry into quiescence, which ensures long-term viability for the cells or in other cases mycelial growth in the dimorphic species, which, in turn, provides the cells with fresh nutrients. Several genes, such as the genes of cAMP cascade, are involved in glucose sensing and response. Since this signal transduction pathway seemed to be an evolutionarily conserved process, we assumed that its genes were also conserved and preserved their functional homology...
March 24, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28341698/schizosaccharomyces-pombe-muts%C3%AE-and-mutl%C3%AE-maintain-stability-of-tetra-nucleotide-repeats-and-msh3-of-hepta-nucleotide-repeats
#16
Desirée Villahermosa, Olaf Christensen, Karen Knapp, Oliver Fleck
Defective mismatch repair (MMR) in humans is associated with colon cancer and instability of microsatellites, that is, DNA sequences with one or several nucleotides repeated. Key factors of eukaryotic MMR are the heterodimers MutSα (Msh2-Msh6), which recognizes base-base mismatches and unpaired nucleotides in DNA, and MutLα (Mlh1-Pms1), which facilitates downstream steps. In addition, MutSβ (Msh2-Msh3) recognizes DNA loops of various sizes, although our previous data and the data presented here suggest that Msh3 of Schizosaccharomyces pombe does not play a role in MMR...
May 5, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28338982/rna-editing-during-sexual-development-occurs-in-distantly-related-filamentous-ascomycetes
#17
Ines Teichert, Tim A Dahlmann, Ulrich Kück, Minou Nowrousian
RNA editing is a post-transcriptional process that modifies RNA molecules leading to transcript sequences that differ from their template DNA. A-to-I editing was found to be widely distributed in nuclear transcripts of metazoa, but was detected in fungi only recently in a study of the filamentous ascomycete Fusarium graminearum that revealed extensive A-to-I editing of mRNAs in sexual structures (fruiting bodies). Here, we searched for putative RNA editing events in RNA-seq data from Sordaria macrospora and Pyronema confluens, two distantly related filamentous ascomycetes, and in data from the Taphrinomycete Schizosaccharomyces pombe...
April 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28338873/molecular-dissection-of-the-actin-binding-ability-of-the-fission-yeast-%C3%AE-actinin-ain1-in-vitro-and-in-vivo
#18
Rikuri Morita, Masak Takaine, Osamu Numata, Kentaro Nakano
A contractile ring (CR) is involved in cytokinesis in animal and yeast cells. Although several types of actin-bundling proteins associate with F-actin in the CR, their individual roles in the CR have not yet been elucidated in detail. Ain1 is the sole α-actinin homologue in the fission yeast Schizosaccharomyces pombe and specifically localizes to the CR with a high turnover rate. S. pombe cells lacking the ain1+ gene show defects in cytokinesis under stress conditions. We herein investigated the biochemical activity and cellular localization mechanisms of Ain1...
February 28, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28292918/nonhomologous-end-joining-with-minimal-sequence-loss-is-promoted-by-the-mre11-rad50-nbs1-ctp1-complex-in-schizosaccharomyces-pombe
#19
Yanhui Li, Jinyu Wang, Gang Zhou, Michael Lajeunesse, Nga Le, Brittany N Stawicki, Yalitza Lopez Corcino, Kathleen L Berkner, Kurt W Runge
While the Mre11-Rad50-Nbs1 (MRN) complex has known roles in repair processes like homologous recombination and microhomology-mediated end-joining, its role in nonhomologous end-joining (NHEJ) is unclear as Saccharomyces cerevisiae, Schizosaccharomyces pombe, and mammals have different requirements for repairing cut DNA ends. Most double-strand breaks (DSBs) require nucleolytic processing prior to DNA ligation. Therefore, we studied repair using the Hermes transposon, whose excision leaves a DSB capped by hairpin ends similar to structures generated by palindromes and trinucleotide repeats...
May 2017: Genetics
https://www.readbyqxmd.com/read/28287547/spatiotemporal-analysis-of-cytokinetic-events-in-fission-yeast
#20
Bin Wei, Brian S Hercyk, Julius Habiyaremye, Maitreyi Das
Cytokinesis, the final step in cell division is critical for maintaining genome integrity. Proper cytokinesis is important for cell differentiation and development. Cytokinesis involves a series of events that are well coordinated in time and space. Cytokinesis involves the formation of an actomyosin ring at the division site, followed by ring constriction, membrane furrow formation and extra cellular matrix remodeling. The fission yeast, Schizosaccharomyces pombe (S. pombe) is a well-studied model system that has revealed with substantial clarity the initial events in cytokinesis...
February 20, 2017: Journal of Visualized Experiments: JoVE
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