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https://www.readbyqxmd.com/read/28725905/schizosaccharomyces-pombe-grx4-regulates-the-transcriptional-repressor-php4-via-2fe-2s-cluster-binding
#1
Adrienne C Dlouhy, Jude Beaudoin, Simon Labbé, Caryn E Outten
The fission yeast Schizosaccharomyces pombe expresses the CCAAT-binding factor Php4 in response to iron deprivation. Php4 forms a transcription complex with Php2, Php3, and Php5 to repress the expression of iron proteins as a means to economize iron usage. Previous in vivo results demonstrate that the function and location of Php4 are regulated in an iron-dependent manner by the cytosolic CGFS type glutaredoxin Grx4. In this study, we aimed to biochemically define these protein-protein and protein-metal interactions...
July 20, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28718400/histone-h3g34r-mutation-causes-replication-stress-homologous-recombination-defects-and-genomic-instability-in-s-pombe
#2
Rajesh K Yadav, Carolyn M Jablonowski, Alfonso G Fernandez, Brandon R Lowe, Ryan A Henry, David Finkelstein, Kevin J Barnum, Alison L Pidoux, Yin-Ming Kuo, Jie Huang, Matthew J O'Connell, Andrew J Andrews, Arzu Onar-Thomas, Robin C Allshire, Janet F Partridge
Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express only the mutant histone H3. H3-G34R specifically reduces H3K36 tri-methylation and H3K36 acetylation, and mutants show partial transcriptional overlap with set2 deletions. H3-G34R mutants exhibit genomic instability and increased replication stress, including slowed replication fork restart, although DNA replication checkpoints are functional...
July 18, 2017: ELife
https://www.readbyqxmd.com/read/28697384/schizosaccharomyces-pombe-and-saccharomyces-cerevisiae-yeasts-in-sequential-fermentations-effect-on-phenolic-acids-of-fermented-kei-apple-dovyalis-caffra-l-juice
#3
P P Minnaar, N P Jolly, V Paulsen, H W Du Plessis, M Van Der Rijst
Kei-apple (Dovyalis caffra) is an evergreen tree indigenous to Southern Africa. The fruit contains high concentrations of l-malic acid, ascorbic acid, and phenolic acids. Kei-apple juice was sequentially inoculated with Schizosaccharomyces pombe and Saccharomyces cerevisiae yeasts. A reference fermentation using only S. cerevisiae was included. The fermentation was monitored by recording mass loss. At the end of fermentation, twelve untrained judges conducted free choice aroma profiling on the fruit wines. The Kei-apple juice and wines were analysed for total titratable acidity, total soluble solids, pH, alcohol, l-malic acid, and phenolic acids...
July 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28682306/unique-roles-for-histone-h3k9me-states-in-rnai-and-heritable-silencing-of-transcription
#4
Gloria Jih, Nahid Iglesias, Mark A Currie, Natarajan V Bhanu, Joao A Paulo, Steven P Gygi, Benjamin A Garcia, Danesh Moazed
Heterochromatic DNA domains play important roles in regulation of gene expression and maintenance of genome stability by silencing repetitive DNA elements and transposons. From fission yeast to mammals, heterochromatin assembly at DNA repeats involves the activity of small noncoding RNAs (sRNAs) associated with the RNA interference (RNAi) pathway(1-9). Typically, sRNAs, originating from long noncoding RNAs, guide Argonaute-containing effector complexes to complementary nascent RNAs to initiate histone H3 lysine 9 di- and tri-methylation (H3K9me2 and H3K9me3, respectively) and heterochromatin formation(10-17)...
June 22, 2017: Nature
https://www.readbyqxmd.com/read/28671615/torc1-dependent-phosphorylation-targets-in-fission-yeast
#5
REVIEW
Yoko Otsubo, Akio Nakashima, Masayuki Yamamoto, Akira Yamashita
Target of rapamycin (TOR) kinase controls cell metabolism and growth in response to environmental cues such as nutrients, growth factors, and stress. TOR kinase is widely conserved across eukaryotes. As in other organisms, the fission yeast Schizosaccharomyces pombe has two types of TOR complex, namely TOR complex 1 (TORC1) and TORC2. It is interesting that the two TOR complexes in S. pombe have opposite roles in sexual differentiation, which is induced by nutrient starvation. TORC1, which contains Tor2 as a catalytic subunit, promotes vegetative growth and represses sexual differentiation in nutrient-rich conditions, while TORC2 is required for the initiation of sexual differentiation...
July 3, 2017: Biomolecules
https://www.readbyqxmd.com/read/28667014/the-gluconate-shunt-is-an-alternative-route-for-directing-glucose-into-the-pentose-phosphate-pathway-in-fission-yeast
#6
Mark E Corkins, Stevin Wilson, Jean-Christophe Cocuron, Ana P Alonso, Amanda J Bird
Glycolysis and the pentose phosphate pathway both play a central role in the degradation of glucose in all domains of life. Another metabolic route that can facilitate glucose breakdown is the gluconate shunt. In this shunt glucose dehydrogenase and gluconate kinase catalyze the two-step conversion of glucose into the pentose phosphate pathway intermediate 6-phosphogluconate. Despite the presence of these enzymes in many organisms, their only established role is in the production of 6-phosphogluconate for the Entner-Doudoroff pathway...
June 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28657616/efficient-conversion-of-n-6-threonylcarbamoyladenosine-t-6-a-into-a-trna-native-hydantoin-cyclic-form-ct-6-a-performed-at-nucleoside-and-oligoribonucleotide-levels
#7
Michal Matuszewski, Katarzyna Debiec, Elzbieta Sochacka
A t(6)A nucleoside was efficiently and stereospecifically transformed into a hydantoin cyclic form of N(6)-l-threonylcarbamoyladenosine (ct(6)A) by the use of polymer bounded carbodiimide (EDC-P) and HOBt. The procedure was successfully applied for a post-synthetic conversion of t(6)A-containing RNA 17-mers (of the sequences of anticodon stem and loop (ASL) fragments of S. pombe tRNA(i) and E. coli tRNA(Lys)) into the products bearing the ct(6)A unit.
June 28, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28645196/antagonistic-regulation-of-cyclin-expression-by-the-bzip-transcription-factors-pcr1-and-atf1-during-g2-m-transition
#8
Sushobhana Bandyopadhyay, Protiti Maiti Ghosh, Sohini Basu, Madhurima Paul, Syed Benazir Alam, Elizabeth Das, Geetanjali Sundaram
The transcription factor Atf1 is known to promote cell survival during various stress conditions in Schizosaccharomyces pombe by activating the expression of appropriate genes. It can also activate transcription of other important genes responsible for cell cycle progression. An Atf1 dependent increase in the expression of cell division promoting genes will oppose activation of checkpoints necessary to ensure repairs and cell survival during stress. Hence selective inhibition of the cell cycle related functions of Atf1 would be indispensable for cellular survival during stress...
June 22, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28639147/rethinking-cell-cycle-dependent-gene-expression-in-schizosaccharomyces-pombe
#9
Stephen Cooper
Three studies of gene expression during the division cycle of Schizosaccharomyces pombe led to the proposal that a large number of genes are expressed at particular times during the S. pombe cell cycle. Yet only a small fraction of genes proposed to be expressed in a cell-cycle-dependent manner are reproducible in all three published studies. In addition to reproducibility problems, questions about expression amplitudes, cell-cycle timing of expression, synchronization artifacts, and the problem with methods for synchronizing cells must be considered...
June 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28636937/the-conserved-rna-binding-cyclophilin-rct1-regulates-small-rna-biogenesis-and-splicing-independent-of-heterochromatin-assembly
#10
An-Yun Chang, Stephane E Castel, Evan Ernst, Hyun Soo Kim, Robert A Martienssen
RNAi factors and their catalytic activities are essential for heterochromatin assembly in S. pombe. This has led to the idea that siRNAs can promote H3K9 methylation by recruiting the cryptic loci regulator complex (CLRC), also known as recombination in K complex (RIKC), to the nucleation site. The conserved RNA-binding protein Rct1 (AtCyp59/SIG-7) interacts with splicing factors and RNA polymerase II. Here we show that Rct1 promotes processing of pericentromeric transcripts into siRNAs via the RNA recognition motif...
June 20, 2017: Cell Reports
https://www.readbyqxmd.com/read/28634040/1-2-4-triazole-and-1-3-4-oxadiazole-analogues-synthesis-mo-studies-in-silico-molecular-docking-studies-antimalarial-as-dhfr-inhibitor-and-antimicrobial-activities
#11
Sampark S Thakkar, Parth Thakor, Hiren Doshi, Arabinda Ray
1,2,4-Triazole and 1,3,4-oxadiazole analogues are of interest due to their potential activity against microbial and malarial infections. In search of suitable antimicrobial and antimalarial compounds, we report here the synthesis, characterization and biological activities of 1,2,4-triazole and 1,3,4-oxadiazole analogues (SS 1-SS 10). The molecules were characterized by IR, mass, (1)H NMR, (13)C NMR and elemental analysis. The in vitro antimicrobial activity was investigated against pathogenic strains, the results were explained with the help of DFT and PM6 molecular orbital calculations...
May 27, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28632741/aging-mortality-and-the-fast-growth-trade-off-of-schizosaccharomyces-pombe
#12
Hidenori Nakaoka, Yuichi Wakamoto
Replicative aging has been demonstrated in asymmetrically dividing unicellular organisms, seemingly caused by unequal damage partitioning. Although asymmetric segregation and inheritance of potential aging factors also occur in symmetrically dividing species, it nevertheless remains controversial whether this results in aging. Based on large-scale single-cell lineage data obtained by time-lapse microscopy with a microfluidic device, in this report, we demonstrate the absence of replicative aging in old-pole cell lineages of Schizosaccharomyces pombe cultured under constant favorable conditions...
June 2017: PLoS Biology
https://www.readbyqxmd.com/read/28631612/wtf-genes-are-prolific-dual-poison-antidote-meiotic-drivers
#13
Nicole L Nuckolls, María Angélica Bravo Núñez, Michael T Eickbush, Janet M Young, Jeffrey J Lange, Jonathan S Yu, Gerald R Smith, Sue L Jaspersen, Harmit S Malik, Sarah E Zanders
Meiotic drivers are selfish genes that bias their transmission into gametes, defying Mendelian inheritance. Despite the significant impact of these genomic parasites on evolution and infertility, few meiotic drive loci have been identified or mechanistically characterized. Here, we demonstrate a complex landscape of meiotic drive genes on chromosome 3 of the fission yeasts Schizosaccharomyces kambucha and S. pombe. We identify S. kambucha wtf4 as one of these genes that acts to kill gametes (known as spores in yeast) that do not inherit the gene from heterozygotes...
June 20, 2017: ELife
https://www.readbyqxmd.com/read/28631610/a-large-gene-family-in-fission-yeast-encodes-spore-killers-that-subvert-mendel-s-law
#14
Wen Hu, Zhao-Di Jiang, Fang Suo, Jin-Xin Zheng, Wan-Zhong He, Li-Lin Du
Spore killers in fungi are selfish genetic elements that distort Mendelian segregation in their favor. It remains unclear how many species harbor them and how diverse their mechanisms are. Here, we discover two spore killers from a natural isolate of the fission yeast Schizosaccharomyces pombe. Both killers belong to the previously uncharacterized wtf gene family with 25 members in the reference genome. These two killers act in strain-background-independent and genome-location-independent manners to perturb the maturation of spores not inheriting them...
June 20, 2017: ELife
https://www.readbyqxmd.com/read/28625541/plant-derived-antifungal-compounds-trigger-a-common-transcriptional-response
#15
Zhe Wang
Understanding the mechanism of action of antifungal drugs is vital for better control of mycosis, which kills >1.3 million lives every year thus remains a major health problem worldwide. In this study, we investigate the activities of three different categories of plant-derived antifungal compounds (resveratrol, honokiol and osthole) via transcriptomics and bioinformatics analysis, with the goal of discovering the common Mode-of-Action (MoA) at molecular level. The result shows that a common transcriptional response (72 gene are up-regulated while 10 genes are down-regulated, commonly) are triggered by above representative antifungal compounds in Schizosaccharomyces pombe (S...
June 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28619713/molecular-model-of-fission-yeast-centrosome-assembly-determined-by-superresolution-imaging
#16
Andrew J Bestul, Zulin Yu, Jay R Unruh, Sue L Jaspersen
Microtubule-organizing centers (MTOCs), known as centrosomes in animals and spindle pole bodies (SPBs) in fungi, are important for the faithful distribution of chromosomes between daughter cells during mitosis as well as for other cellular functions. The cytoplasmic duplication cycle and regulation of the Schizosaccharomyces pombe SPB is analogous to centrosomes, making it an ideal model to study MTOC assembly. Here, we use superresolution structured illumination microscopy with single-particle averaging to localize 14 S...
June 15, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28572211/stable-isotope-labeling-by-amino-acids-in-cell-culture-silac-technology-in-fission-yeast
#17
Boris Maček, Alejandro Carpy, André Koch, Claudia C Bicho, Weronika E Borek, Silke Hauf, Kenneth E Sawin
Shotgun proteomics combined with stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach to quantify proteins and posttranslational modifications across the entire proteome. SILAC technology in Schizosaccharomyces pombe must cope with the "arginine conversion problem," in which isotope-labeled arginine is converted to other amino acids. This can be circumvented by either using stable isotope-marked lysine only (as opposed to the more standard lysine/arginine double labeling) or using yeast genetics to create strains that only very inefficiently convert arginine...
June 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28555368/a-mutated-dph3-gene-causes-sensitivity-of-schizosaccharomyces-pombe-cells-to-cytotoxic-agents
#18
Desirée Villahermosa, Karen Knapp, Oliver Fleck
Dph3 is involved in diphthamide modification of the eukaryotic translation elongation factor eEF2 and in Elongator-mediated modifications of tRNAs, where a 5-methoxycarbonyl-methyl moiety is added to wobble uridines. Lack of such modifications affects protein synthesis due to inaccurate translation of mRNAs at ribosomes. We have discovered that integration of markers at the msh3 locus of Schizosaccharomyces pombe impaired the function of the nearby located dph3 gene. Such integrations rendered cells sensitive to the cytotoxic drugs hydroxyurea and methyl methanesulfonate...
May 29, 2017: Current Genetics
https://www.readbyqxmd.com/read/28528978/multimodal-control-of-transcription-factor-pap1-in-schizosaccharomyces-pombe-under-nitrosative-stress
#19
Puranjoy Kar, Pranjal Biswas, Sanjay Ghosh
Schizosaccharomyces pombe Pap1, a bZIP transcription factor, is highly homologous to the mammalian c-Jun protein that belongs to the AP1 family of transcriptional regulators. The role of transcription factor Pap1 has been extensively studied under oxidative stress. Two cysteine residues in Pap1p namely, C278 and C501 form disulfide linkage under oxidative stress resulting in nuclear accumulation. We first time showed the involvement of Pap1 in the protection against nitrosative stress. In the present study we show that pap1 deletion makes growth of S...
July 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28522285/production-of-3-hydroxypropionic-acid-via-the-malonyl-coa-pathway-using-recombinant-fission-yeast-strains
#20
Akiko Suyama, Yujiro Higuchi, Masahiro Urushihara, Yuka Maeda, Kaoru Takegawa
3-Hydroxypropionic acid (3-HP) can be converted into derivatives such as acrylic acid, a source for producing super absorbent polymers. Although Escherichia coli has often been used for 3-HP production, it exhibits low tolerance to 3-HP. To circumvent this problem, we selected the fission yeast Schizosaccharomyces pombe as this microorganism has higher tolerance to 3-HP than E. coli. Therefore, we constructed S. pombe transformants overexpressing two genes, one encoding the S. pombe acetyl-CoA carboxylase (Cut6p) and the other encoding the malonyl-CoA reductase derived from Chloroflexus aurantiacus (CaMCR)...
May 15, 2017: Journal of Bioscience and Bioengineering
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