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tuberculosis presentation

Linlin Li, Yonghua Yuan, Yongjie Chen, Pu Zhang, Yan Bai, Lijuan Bai
The 6-kDa early secretory antigenic target referred to as ESAT-6 is a virulence factor secreted by Mycobacterium tuberculosis (MTB). This work describes a voltammetric aptasensor for ultrasensitive detection of ESAT-6. Reduced graphene oxide doped with metal-organic framework (MOF-rGO) was deposited on a glassy carbon electrode (GCE). This increases the immobilization of electroactive Toluidine Blue (TB) and facilitates the electron transfer from TB to the modified GCE. Platinum/gold core/shell (Pt@Au) nanoparticles were used to assemble thiolated ESAT-6 binding aptamer (EBA) on a modified electrode and to further amplify the response to TB...
July 17, 2018: Mikrochimica Acta
Ruchi Rathore, Shivangi Chauhan, Suman Mendiratta, Ritu Sharma, Manupriya Nain, Namrata Sarin
Xanthogranulomatous inflammation is a rare form of chronic inflammatory response consisting of macrophages, lymphocytes, plasma cells and neutrophils too. Due to its locally destructive nature and mass forming capacity as a result of adhesions, this type of inflammation may mimic malignancy or tuberculosis both clinically and radiologically. We present a report of two such cases, one mimicking tuberculosis and the other mimicking malignancy clinically. Awareness of this condition and a higher index of suspicion among clinicians, radiologists and pathologists can help in early diagnosis and more appropriate treatment of this potentially destructive disorder...
September 2017: Journal of Family & Reproductive Health
Diana Machado, Miriam Girardini, Miguel Viveiros, Marco Pieroni
The emergence of multi- and extensively drug resistant tuberculosis worldwide poses a great threat to human health and highlight the need to discover and develop new, effective and inexpensive antituberculosis agents. High-throughput screening assays against well-validated drug targets and structure based drug design have been employed to discover new lead compounds. However, the great majority fail to demonstrate any antimycobacterial activity when tested against Mycobacterium tuberculosis in whole-cell screening assays...
2018: Frontiers in Microbiology
Landry Blanc, Jansy Passiflora Sarathy, Nadine Alvarez Cabrera, Paul O'Brien, Isabela Dias-Freedman, Marizel Mina, James Sacchettini, Radojka M Savic, Martin Gengenbacher, Brendan K Podell, Brendan Prideaux, Thomas Ioerger, Thomas Dick, Véronique Dartois
In the 1970s, inclusion of pyrazinamide (PZA) in the drug regimen of tuberculosis (TB) patients for the first 2 mo achieved a drastic reduction of therapy duration. Until now, however, the mechanisms underlying PZA's unique contribution to efficacy have remained controversial, and animal efficacy data vary across species. To understand how PZA kills bacterial populations present in critical lung lesion compartments, we first characterized a rabbit model of active TB, showing striking similarities in lesion types and fates to nonhuman primate models deemed the most appropriate surrogates of human TB...
July 17, 2018: Journal of Experimental Medicine
A Bleibtreu, N Grall, J-P Laissy, C Rioux, A Strukov, S Lariven, P Yeni, Y Yazdanpanah, V Joly
BACKGROUND: Miliary tuberculosis (miliary TB) is characterized by a hematogenous spread of Mycobacterium tuberculosis. Cerebral lesions associated with miliary TB have been reported with diverse frequencies. METHODS: We retrospectively analyzed brain imaging in 34 patients presenting with proven miliary TB hospitalized in our teaching hospital between 2008 and 2014. RESULTS: Neurological symptoms were present at admission in 15 patients, emerged during treatment in six, and were never reported in 13...
July 12, 2018: Médecine et Maladies Infectieuses
Priyatam Khadka, Ramesh Bahadur Basnet, Basista Parsad Rijal, Jeevan Bahadur Sherchand
BACKGROUND: Pulmonary nocardiosis is an opportunistic infection in an immunocompromised patient; however, often neglected in the immunocompetent patient from the diagnosis considerations. CASE PRESENTATIONS: We describe a case of pulmonary nocardiosis masquerading renascence of tuberculosis, in a 51-years-Nepali farmer. After a 6 month of presumed successful antitubercular therapy; the patient develops the clinical presentations and radiological features showing similarities with that of tuberculosis and malignancy...
July 17, 2018: BMC Research Notes
Yassir A Shuaib, Eltahir A G Khalil, Ulrich E Schaible, Lothar H Wieler, Mohammed A M Bakheit, Saad E Mohamed-Noor, Mohamed A Abdalla, Susanne Homolka, Sönke Andres, Doris Hillemann, Knut Lonnroth, Elvira Richter, Stefan Niemann, Katharina Kranzer
Background: In Sudan, tuberculosis diagnosis largely relies on clinical symptoms and smear microscopy as in many other low- and middle-income countries. The aim of this study was to investigate the positive predictive value of a positive sputum smear in patients investigated for pulmonary tuberculosis in Eastern Sudan. Methods: Two sputum samples from patients presenting with symptoms suggestive of tuberculosis were investigated using direct Ziehl-Neelsen (ZN) staining and light microscopy between June to October 2014 and January to July 2016...
2018: Tuberculosis Research and Treatment
Bipadabhanjan Mallick, Harshal S Mandavdhare, Sourabh Aggarwal, Harjeet Singh, Usha Dutta, Vishal Sharma
Background: Chylous ascites is an uncommon presentation of mycobacterial infection. Methods: We report three cases of tubercular chylous ascites, and in addition, we performed a systematic review of the published literature for the clinical presentation, treatment, and outcomes of mycobacterial chylous ascites. We followed the PRISMA guidelines for the systematic review. Results: A total of 33 cases (including three of ours) were included...
July 2018: Therapeutic Advances in Infectious Disease
Pauraic McCarron, Malachy McCann, Michael Devereux, Kevin Kavanagh, Ciaran Skerry, Petros C Karakousis, Ana C Aor, Thaís P Mello, André L S Santos, Débora L Campos, Fernando R Pavan
Mycobacterium tuberculosis is the etiologic agent of tuberculosis. The demand for new chemotherapeutics with unique mechanisms of action to treat (multi)resistant strains is an urgent need. The objective of this work was to test the effect of manganese(II) and copper(II) phenanthroline/dicarboxylate complexes against M. tuberculosis . The water-soluble Mn(II) complexes, [Mn2 (oda)(phen)4 (H2 O)2 ][Mn2 (oda)(phen)4 (oda)2 ]·4H2 O ( 1 ) and {[Mn(3,6,9-tdda)(phen)2 ]·3H2 O·EtOH}n ( 3 ) (odaH2 = octanedioic acid, phen = 1,10-phenanthroline, tddaH2 = 3,6,9-trioxaundecanedioic acid), and water-insoluble complexes, [Mn(ph)(phen)(H2 O)2 ] ( 5 ), [Mn(ph)(phen)2 (H2 O)]·4H2 O ( 6 ), [Mn2 (isoph)2 (phen)3 ]·4H2 O ( 7 ), {[Mn(phen)2 (H2 O)2 ]}2 (isoph)2 (phen)·12H2 O ( 8 ) and [Mn(tereph)(phen)2 ]·5H2 O ( 9 ) (phH2 = phthalic acid, isophH2 = isophthalic acid, terephH2 = terephthalic acid), robustly inhibited the viability of M...
2018: Frontiers in Microbiology
Yvonne A Dei-Adomakoh, Leslie Quarcoopome, Afua D Abrahams, Catherine I Segbefia, Dzifa I Dey
Castleman disease is a rare cause of lymphoid hyperplasia and may result in localized symptoms or an aggressive, multisystem disorder. It can mimic other diseases like lymphoma or tuberculosis. It classically presents as a mediastinal mass that involves the lymphatic tissue primarily but can also affect extra lymphatic sites including the lungs, larynx, parotid glands, pancreas, meninges, and muscles. In HIV and HHV8-negative patients with idiopathic multi-centric Castleman disease, pathogenesis may involve autoimmune mechanisms...
March 2018: Ghana Medical Journal
Harshal S Mandavdhare, Vishal Sharma, Harjeet Singh, Usha Dutta
Chylous ascites is an uncommon entity and infectious etiology is the most common cause in developing countries. However, recently, whether there is any change in trend of etiologies in developing countries is not known. In this study, a retrospective analysis of the data of cases of atraumatic chylous ascites was conducted. Twelve patients of atraumatic chylous ascites with a mean age of 35 years were studied and 6 of them were males. The mean duration of symptoms was 9.6 months and the clinical presentation was abdominal distension (12 cases), pain abdomen (10 cases), loss of appetite and weight (9 cases), peripheral lymphadenopathy (4 cases) and fever (3 cases)...
July 15, 2018: Bioscience Trends
George Antunes, Karen Galloway, Nigel Clerk, Richard Hartley
Human bovine tuberculosis is a rare zoonotic infection in developed countries which has been achieved predominantly by effective eradication programmes in cattle. The principal modes of transmission are consumption of unpasteurised dairy products and close contact with infected cattle. The clinical and radiological presentation is indistinguishable from tuberculosis caused by Mycobacterium tuberculosis The diagnosis should be considered in individuals with relevant risk factors who present with intra/extrathoracic pathology...
July 16, 2018: BMJ Case Reports
Takahiro Nagatake, Hidehiko Suzuki, So-Ichiro Hirata, Naomi Matsumoto, Yasuko Wada, Sakiko Morimoto, Ayaka Nasu, Michiko Shimojou, Mitsuo Kawano, Kentaro Ogami, Yusuke Tsujimura, Etsushi Kuroda, Norifumi Iijima, Koji Hosomi, Ken J Ishii, Tetsuya Nosaka, Yasuhiro Yasutomi, Jun Kunisawa
We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of inducible bronchus-associated lymphoid tissue (iBALT) played a role in the induction of antigen-specific T cells and IgA antibody responses in the lung of mice intranasally administered Ag85B-rHPIV2. We found that expression of Ag85B was dispensable for the development of iBALT, suggesting that HPIV2 acted as iBALT-inducing vector...
July 16, 2018: International Immunology
S Agarwal, S Ghosh, S Sharma, K Kaur, I Verma
Mycobacterium tuberculosis survives within the infected host cells by escaping phago-lysosomal fusion and adaptation to hosts' intracellular milieu. Recently, role of alveolar epithelial cells (AECs) in survival of intracellular M. tuberculosis is gaining importance. It has been reported that M. tuberculosis causes cytotoxicity in AECs, a phenotype attributed to M. tuberculosis virulence that could be due to differential gene/protein expression of bacilli in these cells. Thus, present study was focused on comparative proteomic analysis of intracellular mycobacteria within macrophages and epithelial cells...
July 11, 2018: Pathogens and Disease
Arjang Hassibi, Arun Manickam, Rituraj Singh, Sara Bolouki, Ruma Sinha, Kshama B Jirage, Mark W McDermott, Babak Hassibi, Haris Vikalo, Gelareh Mazarei, Lei Pei, Luc Bousse, Mark Miller, Mehrdad Heshami, Michael P Savage, Michael T Taylor, Nader Gamini, Nicholas Wood, Pallavi Mantina, Patrick Grogan, Peter Kuimelis, Piyush Savalia, Scott Conradson, Yuan Li, Rich B Meyer, Edmond Ku, Jessica Ebert, Benjamin A Pinsky, Gregory Dolganov, Tran Van, Kirsten A Johnson, Pejman Naraghi-Arani, Robert G Kuimelis, Gary Schoolnik
The emergence of pathogens resistant to existing antimicrobial drugs is a growing worldwide health crisis that threatens a return to the pre-antibiotic era. To decrease the overuse of antibiotics, molecular diagnostics systems are needed that can rapidly identify pathogens in a clinical sample and determine the presence of mutations that confer drug resistance at the point of care. We developed a fully integrated, miniaturized semiconductor biochip and closed-tube detection chemistry that performs multiplex nucleic acid amplification and sequence analysis...
July 16, 2018: Nature Biotechnology
Julia Bespyatykh, Alexander Smolyakov, Andrei Guliaev, Egor Shitikov, Georgij Arapidi, Ivan Butenko, Marine Dogonadze, Olga Manicheva, Elena Ilina, Victor Zgoda, Vadim Govorun
Nowadays proteomics is one of the major instruments for editing and correcting annotation of genomic information. The correct genome annotation is necessary for omics studies of clinically relevant pathogens like Mycobacterium tuberculosis as well as for the progress in drug design and in silico biology. Here, we focused on the proteogenomic analysis of W-148 strain belonging to the Beijing B0/W148 cluster. This cluster, also known as a "successful" clone possesses unique pathogenic properties and has a unique genome organization...
July 13, 2018: Journal of Proteomics
P Arulmozhi, S Vijayakumar, T Kumar
The aim of the present study is to investigate the antimicrobial potency of leaves from various extracts of Capparis zeylanica, Streblus asper and Tribulus terrestris were evaluated. In addition, this is the first report on MIC, MBC/MFC antimicrobial activities of above mentioned plants and also identify the phytochemical, functional groups by GC-MS and FT-IR respectively. Soxhlet extraction method was used for preparation of different extracts viz., aqueous, petroleum ether, ethyl acetate and methanol. The extracts were examined against Staphylococcus epidermidis, Enterococcus faecallis, Salmonella paratyphi, Shigella dysenteriae, Candida albicans and Mycobacterium tuberculosis by agar well diffusion method, and Minimum Inhibitory Concentratioon (MIC), Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) values were determined through micro dilution method...
July 12, 2018: Microbial Pathogenesis
Daniele M Pelissari, Fredi A Diaz-Quijano
Background: Alcohol and illicit drugs are associated with the discontinuation of tuberculosis (TB) treatment and can compromise the immune system. We estimated the impact of alcohol disorder and the use of illicit drug on TB treatment outcomes, considering the interaction of both substances in patients from São Paulo state, Brazil. Methods: This is a retrospective cohort of patients diagnosed with TB from 2011 to 2015. We estimated the relative risk (RR) of an unsuccessful outcome associated with alcohol disorder, use of illicit drugs and their interaction using a multiple regression model...
2018: Archives of Public Health, Archives Belges de Santé Publique
Sylvia Annabel Dass, Mohd Nor Norazmi, Armando Acosta Dominguez, Maria Elena Sarmiento Garcia San Miguel, Gee Jun Tye
The discovery of heat shock protein 16 kDa antigen protein has deepen the understanding of latent tuberculosis since it was found to be primarily expressed by Mycobacterium tuberculosis during latent phase leading to the rapid optimization and development in terms of diagnosis and therapeutics. Recently, T cell receptor-like antibody has been explored extensively targeting various diseases due to its dual functionality (T cell receptor and antibody). In this study, a TCR-like domain antibody (A2/Ab) with the binding capacity to Mtb heat shock protein (HSP) 16 kDa antigen presented by major histocompatible complex (MHC) HLA-A*02 was successfully generated via biopanning against human domain antibody library...
July 4, 2018: Molecular Immunology
Mercedes Giralt, Amalia Molinero, Juan Hidalgo
Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55 ) + complete Freund's adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE...
2018: Methods in Molecular Biology
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