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https://www.readbyqxmd.com/read/29306950/immune-consequences-of-in-vitro-infection-of-human-peripheral-blood-leukocytes-with-vesicular-stomatitis-virus
#1
Tomasz Tomczyk, Grażyna Wróbel, Radosław Chaber, Iwona Siemieniec, Egbert Piasecki, Małgorzata Krzystek-Korpacka, Beata U Orzechowska
BACKGROUND: Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. Our study aimed to assess the overall immunological consequences of ex vivo infection of PBLs with VSV. METHODS: Phenotypic analysis of lymphocyte subsets and apoptosis were evaluated with flow cytometry. Caspase 3/7 activity was detected by luminescence assay...
January 6, 2018: Journal of Innate Immunity
https://www.readbyqxmd.com/read/29302022/effect-of-immunosuppression-on-target-blood-immune-cells-within-1-year-after-lung-transplantation-influence-of-age-on-t-lymphocytes
#2
Benjamin Coiffard, Matthieu Pelardy, Anderson D Loundou, Corine Nicolino-Brunet, Pascal Alexandre Thomas, Laurent Papazian, Françoise Dignat-George, Martine Reynaud-Gaubert
BACKGROUND Lymphocytes are targeted by immunosuppressive therapy in solid organ transplantation and they influence allograft outcome. MATERIAL AND METHODS Peripheral blood lymphocyte subsets (PBLS) determined by flow cytometry during the first year post-transplant from patients who underwent a first lung transplantation in a French University Hospital between December 2011 and July 2013 were retrospectively analyzed according to recipient characteristics and allograft outcome. RESULTS Fifty-seven recipients were enrolled and 890 PBLS were collected...
January 5, 2018: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/29285089/malp-2-an-agonist-of-tlr6-promotes-the-immune-status-without-affecting-the-differentiation-capacity-of-umbilical-cord-mesenchymal-stem-cells
#3
Xiuli Wu, Lian Xu, Yangmei Shen, Na Yu, Yan Zhang, Tao Guo
Mesenchymal stem cells (MSCs) are increasingly used in cell-based therapy due to their multiple differentiation capacity, low expression of co-stimulatory factors and immunosuppressive effect. However, accumulating studies reported the recognition and rejection of engrafted MSCs, which eventually led to the fail of clinical trials. Toll-like receptors (TLRs) are important in mediating the immune response. In the present study, macrophage-activated lipopeptide-2 (MALP-2) was introduced to activate the TLR6 pathway in umbilical cord MSCs (UCMSCs)...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29284781/dna-damage-in-tumor-cells-and-peripheral-blood-lymphocytes-of-endometrial-cancer-patients-assessed-by-the-comet-assay
#4
L G Buchynska, O V Brieieva, N P Iurchenko, V V Protsenko, S V Nespryadko
To date, genome instability is considered to be a common feature not only of tumor cells, but also of non-malignant cells of cancer patients, including peripheral blood lymphocytes (PBLs). The issue of the association between genome instability in tumor cells and PBLs, as well as of its relationship with tumor progression remains poorly understood. AIM: To evaluate the level DNA damage in tumor cells and PBLs of endometrial cancer (EC) patients with regard to clinical and morphological characteristics of the patients...
December 2017: Experimental Oncology
https://www.readbyqxmd.com/read/29281088/prediction-of-acute-gastrointestinal-and-genitourinary-radiation-toxicity-in-prostate-cancer-patients-using-lymphocyte-microrna
#5
Masanori Someya, Masakazu Hori, Toshio Gocho, Kensei Nakata, Takaaki Tsuchiya, Mio Kitagawa, Tomokazu Hasegawa, Yuuki Fukushima, Koh-Ichi Sakata
Background: To search for novel biomarkers that can predict acute radiation toxicity, we conducted microRNA expression analysis of peripheral blood lymphocytes (PBLs). Methods: The discovery cohort was 69 patients with localized adenocarcinoma of the prostate who received intensity-modulated radiation therapy between October 2007 and October 2010. The validation cohort was 72 patients treated with low-dose-rate brachytherapy between May 2008 and March 2014. After13 microRNAs were selected by TaqMan® Array analysis in a preliminary experiment, expression of these microRNAs in all samples was analyzed by RT-PCR...
December 21, 2017: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29250828/cd4-cd25hi-foxp3-regulatory-t-cells-in-long-term-cardiac-xenotransplantation
#6
Avneesh K Singh, Joshua L Chan, Caleb N Seavey, Philip C Corcoran, Robert F Hoyt, Billeta G T Lewis, Marvin L Thomas, David L Ayares, Keith A Horvath, Muhammad M Mohiuddin
BACKGROUND: CD4+CD25Hi FoxP3+ T (Treg) cells are a small subset of CD4+ T cells that have been shown to exhibit immunoregulatory function. Although the absolute number of Treg cells in peripheral blood lymphocytes (PBL) is very small, they play an important role in suppressing immune reactivity. Several studies have demonstrated that the number of Treg cells, rather than their intrinsic suppressive capacity, may contribute to determining the long-term fate of transplanted grafts. In this study, we analyzed Treg cells in PBL of long-term baboon recipients who have received genetically modified cardiac xenografts from pig donors...
December 17, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29228718/increased-single-strand-annealing-rather-than-non-homologous-end-joining-predicts-hereditary-ovarian-carcinoma
#7
Miriam Deniz, Tatiana Romashova, Sarah Kostezka, Anke Faul, Theresa Gundelach, Maria Moreno-Villanueva, Wolfgang Janni, Thomas W P Friedl, Lisa Wiesmüller
Mutations in genes encoding DNA double-strand break (DSB) repair components, especially homologous recombination (HR) proteins, were found to predispose to breast and ovarian cancer. Beyond high penetrance risk gene mutations underlying monogenic defects, low risk gene mutations generate polygenic defects, enlarging the fraction of individuals with a predisposing phenotype. DSB repair dysfunction opens new options for targeted therapies; poly (ADP-ribose) polymerase (PARP) inhibitors have been approved for BRCA-mutated and platinum-responsive ovarian cancers...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156812/correlation-of-drd2-mrna-expression-levels-with-deficit-syndrome-severity-in-chronic-schizophrenia-patients-receiving-clozapine-treatment
#8
Liang Liu, Yin Luo, Guofu Zhang, Chunhui Jin, Zhenhe Zhou, Zaohuo Cheng, Guozhen Yuan
Schizophrenia is a complex, severe, chronic psychiatric disorder, and the associated deficit syndrome is widely regarded as an important clinical aspect of schizophrenia. This study analyzed the relationship of deficit syndrome severity with the mRNA levels of members of signaling pathways that associate with the pathophysiology of schizophrenia, including the dopamine D2 receptor (DRD2), protein kinase B (AKT1), and phosphoinositide-3 kinase (PI3KCB), in peripheral blood leukocytes (PBLs) of 20 healthy controls and 19 chronic schizophrenia patients with long-term clozapine treatment...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29129999/expression-of-genes-and-their-polymorphism-influences-the-risk-of-knee-osteoarthritis
#9
Abhishek Mishra, Rajeshwar Nath Srivastava, Sachin Awasthi, Devendra Parmar, Priya Mishra
Introduction: Genetic factors including the level of expression of the fingerprint of genes involved in the development of bones and cartilage such as GDF-5 or ESR-α or CALM-1 are known to be strong determinants of the osteoarthritis (OA) in Caucasian and Oriental populations. Because of high prevalence of OA in Indian population and availability of limited genetic data, we determined whether similar genetic factors are involved in Indians as well. Methods: A case control study was carried out involving 500 patients of knee OA and equal number of healthy controls...
2017: Journal of Nucleic Acids
https://www.readbyqxmd.com/read/29104625/high-expression-of-long-chain-acyl-coenzyme-a-synthetase-1-in-peripheral-blood-may-be-a-molecular-marker-for-assessing-the-risk-of-acute-myocardial-infarction
#10
Liping Yang, Yushuang Yang, Daoyuan Si, Kaiyao Shi, Dongna Liu, Heyu Meng, Fanbo Meng
The current study aimed to investigate whether the increased expression of long chain acyl-coenzymeA synthetase 1 (ACSL1) in peripheral blood leukocytes (PBL) may be a molecular marker for the genetic evaluation of acute myocardial infarction (AMI). The mechanism of action of ACSL1 in the pathogenesis of AMI was also investigated. A total of 75 patients with AMI and 70 individuals without coronary heart disease were selected to participate in the present study. The demographic and clinical information of the enrolled subjects was recorded...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29076586/cancer-and-normal-adipose-derived-mesenchymal-stem-cells-ascs-do-they-have-differential-effects-on-tumor-and-immune-cells
#11
Mahboobeh Razmkhah, Zahra Mansourabadi, Maryam Al-Sadat Mohtasebi, Abdol-Rasoul Talei, Abbas Ghaderi
Adipose-derived mesenchymal stem cells (ASCs) are known to have immunomodulatory properties through soluble factors or by direct cell-to-cell contact. This study aimed to assess the expression of HLA-G and IDO activity in breast cancer and normal ASCs and to see if ASC is capable of modulating both tumor cells and immune system cells in vitro. ASCs were enzymatically isolated from 15 breast cancer patients and 10 normal individuals. Then they were cultured, and the impact of their conditioned media on the movement of the MDA-MB-231 breast cancer cell line was studied in wound healing scratch assay...
October 27, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29066482/the-effect-of-metformin-on-the-expression-of-gpr109a-nf-%C3%AE%C2%BAb-and-il-1%C3%AE-in-peripheral-blood-leukocytes-from-patients-with-type-2-diabetes-mellitus
#12
Xiaoling Xu, Shaoda Lin, Yongsong Chen, Xitao Li, Shuhua Ma, Yucai Fu, Chiju Wei, Chang Wang, Wencan Xu
OBJECTIVES: Type 2 Diabetes Mellitus (T2DM), which often accompanies dyslipidemia, is considered an inflammatory disease. GPR109A, as a niacin receptor, is up-regulated under high glucose concentration. Activation of GPR109A reduces GSIS and exerts anti-inflammatory effects by regulating NF-κB/IL-1β signaling. Metformin improves hyperglycemia, increases insulin sensitivity and attenuates the activation of the NF-κB pathway in T2DM. We aimed to examine whether metformin plays beneficial effects in T2DM by regulating the GPR109A signaling...
September 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29045966/-immune-effects-of-specific-ctls-response-induced-by-dendritic-cells-pulsed-with-ny-eso-1-peptide
#13
J W Liu, X Lu, Z M Yang, L J Deng, L Yang
OBJECTIVE: To explore the potential of autologous dendritic cells (DCs) pulsed with caner/testis antigen NY-ESO-1 peptides in inducing specific cytotoxic T lymphocyte (CTLs) response and antineoplastic immune function of specific CTLs. METHODS: Fifteen patients with II to III stage positive HLA -A0201(+) and NY-ESO-1(+) were enrolled in the Cancer Hospital Chinese Academy of Medical Sciences on the basis of preclinical experiments from November 2014 to October 2015, and their peripheral blood mononuclear cells (PBMCs) and peripheral blood lymphocytes (PBLs) were isolated...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29034433/development-of-humanized-scfv-antibody-fragment-s-that-targets-and-blocks-specific-hla-alleles-linked-to-myasthenia-gravis
#14
B Vijayalakshmi Ayyar, M Zouhair Atassi
Myasthenia gravis (MG) is an autoimmune disease caused by sensitization of the immune system to self-antigens. We have previously shown that targeting MG-susceptible alleles can significantly inhibit proliferation of disease-specific T cells. In this work, we humanized a murine monoclonal antibody (mAb) LG11, capable of blocking MG-associated DQ beta 1 (DQB1) allele and reformatted it into single-chain fragment variable (scFv). A fully functional humanized scFv was obtained by optimizing variable domain orientations and linker lengths, along with the optimization of expression conditions and codons to suit Escherichia coli expression machinery...
October 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29029457/prognostic-relevance-of-protein-expression-clinical-factors-and-myd88-mutation-in-primary-bone-lymphoma
#15
Yong Xu, Jian Li, Jian Ouyang, Juan Li, Jingyan Xu, Qiguo Zhang, Yonggong Yang, Min Zhou, Jing Wang, Cuiling Zhang, Yueyi Xu, Ping Li, Rongfu Zhou, Bing Chen
Primary bone lymphomas (PBLs) are composed of malignant lymphoid cells presenting in osseous sites, without supra-regional lymph node or extranodal involvement. We systematically characterized the immunophenotype and the myeloid differentiation factor 88 (MYD88)-L265P gene mutation status in PBL. Clinical data from 19 patients with PBL treated at Nanjing Drum Tower Hospital between 2009 and 2015 were analyzed retrospectively. Protein expression patterns were identified immunohistochemically, and MYD88 mutation was assessed using polymerase chain reaction and direct DNA sequencing...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29024769/identification-of-a-single-p19-gene-and-three-p40-paralogues-in-grass-carp-ctenopharyngodon-idellus-their-potential-for-the-formation-of-interleukin-23-and-inducible-expression-in-vitro-and-in-vivo
#16
Xinyan Wang, Lei Qin, Linyong Du, Di Chen, Anying Zhang, Kun Yang, Hong Zhou
Interleukin (IL-) 23, a member of IL-12 family, is a composite cytokine with the subunits of p19 and p40. Although IL-12 and IL-23 share the p40 subunit, they play vastly different roles in immune regulation. In teleost, much emphasis has been placed on the identification of IL-12, but evidence for the existence of IL-23 is still lacking. In the present study, a p19 gene and three p40 paralogues were isolated and identified from grass carp, suggesting multiple assembly of IL-23 molecules in fish species. To address this issue, the existence of different p19/p40 heterodimers were examined by Co-Immunoprecipitation (Co-IP) assay, showing that only co-expression of p19 and each p40 subunit could produce the soluble proteins corresponding to three IL-23 isoforms...
October 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28988993/characterization-and-functional-assessment-of-the-nlrc3-like-molecule-of-the-goldfish-carassius-auratus-l
#17
Jiasong Xie, Miodrag Belosevic
The NLRC3-like (NLRC3L) molecule from the goldfish transcriptome database was identified and characterized. Quantitative gene expression analysis revealed the highest mRNA levels of NLRC3L were in the spleen and intestine, with lower mRNA levels observed in muscle and liver. Goldfish NLRC3L was differentially expressed in goldfish immune cell populations with highest mRNA levels measured in PBLs and macrophages. We generated a recombinant form of the molecule (rgfNLRC3L) and an anti-CT-NLRC3L IgG. Treatment of goldfish primary kidney macrophages in vitro with ATP, LPS and heat-killed Aeromonas salmonicida up-regulated the NLRC3L mRNA and protein...
October 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28988766/estimation-of-the-future-remnant-liver-function-is-a-better-tool-to-predict-post-hepatectomy-liver-failure-than-platelet-based-liver-scores
#18
T Chapelle, B Op de Beeck, A Driessen, G Roeyen, B Bracke, V Hartman, I Huyghe, S Morrison, D Ysebaert, S Francque
INTRODUCTION: Recently, there has been increasing interest in the preoperative prediction and prevention of post-hepatectomy liver failure (PHLF). This is a particular concern in colorectal liver metastases (CRLM), when surgery follows potentially hepatotoxic chemotherapy. Platelet-based liver scores (PBLS) such as APRI and FIB-4 are predictive of chemotherapy-associated liver injury (CALI) and PHLF. Estimation of the future liver remnant function (eFLRF) by combining (99m)Tc-Mebrofenin Hepatobiliary Scintigraphy (HBS(BSA)) with future liver remnant volume ratio (FLRV%), is predictive of PHLF and related mortality...
September 4, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28946702/astragalus-polysaccharide-protect-against-cadmium-induced-cytotoxicity-through-the-mda5-nf-%C3%AE%C2%BAb-pathway-in-chicken-peripheral-blood-lymphocytes
#19
Wanqiu Xie, Ming Ge, Guangxing Li, Linan Zhang, Zequn Tang, Ruyue Li, Ruili Zhang
Cadmium (Cd) is a known environmental pollutant that is associated with inflammation, oxidative stress, and cell apoptosis. Astragalus polysaccharide (APS) is a major component of Astragalus membranaceus, a vital qi-reinforcing herb medicine with favorable immuneregulation properties. To study the effect of APS on the inhibition of the cadmium-induced injury of peripheral blood lymphocytes (PBLs) in chickens through the MDA5/NF-κB signaling pathway, PLBs acquired from 15-day-old chickens were divided into control group, Cd group, APS + Cd group, anti-MDA5 mAb + Cd group, BAY 11-7082 (a nuclear factor kappa-light chain-enhancer of activated B cells [NF-κB] inhibitor) +Cd group, APS group, anti-MDA5 mAb group, and BAY 11-7082 group...
September 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28944882/evaluation-of-apoptosis-induced-by-exposure-to-antineoplastic-drugs-in-peripheral-blood-lymphocytes-of-nurses
#20
Heng Liao, Lijie Bi, Jun Wei, Xin Song
Cytostatic antineoplastic drugs are considered carcinogenic and mutagenic risk factors for health workers who are occupationally exposed to them; however, the molecular mechanisms underlying these effects remain to be elucidated. Therefore, the present study aimed to investigate the underlying mechanisms of antineoplastic drugs‑induced apoptosis of peripheral blood lymphocytes (PBLs) obtained from oncology nurses handling antineoplastic drugs. A microRNA (miRNA/miR) polymerase chain reaction (PCR) array was performed to analyze the expression levels of miRNAs in the PBLs from 3 trained nurses occupationally exposed to antineoplastic drugs...
December 2017: Molecular Medicine Reports
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