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https://www.readbyqxmd.com/read/27894409/the-effects-of-mitochondrial-dna-deletion-and-copy-number-variations-on-different-exercise-intensities-in-highly-trained-swimmers
#1
O Baykara, S K Sahin, F Akbas, M Guven, I Onaran
It has been suggested that heavy exercise might increase oxidative stress, causing mitochondrial DNA (mtDNA) mutations as well as DNA mutations and changes in the mtDNA copy number in cells. mtDNA4977 deletion is one of the most common deletions seen on mitochondria. We hypothesize association between exercise induced oxidative stress and mtDNA damage in peripheral blood lymphocytes (PBLs) of highly trained swimmers. Therefore we studied the mtDNA4977 deletion level, mtDNA copy number and their relationship with cellular ATP and oxidative stress status in PBLs of swimmers...
October 31, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27878103/role-of-rna-secondary-structure-in-emergence-of-compartment-specific-hepatitis-b-virus-immune-escape-variants
#2
Sibnarayan Datta, Runu Chakravarty
AIM: To investigate the role of subgenotype specific RNA secondary structure in the compartment specific selection of hepatitis B virus (HBV) immune escape mutations. METHODS: This study was based on the analysis of the specific observation of HBV subgenotype A1 in the serum/plasma, while subgenotype A2 with G145R mutation in the peripheral blood leukocytes (PBLs). Genetic variability found among the two subgenotypes was used for prediction and comparison of the full length pregenomic RNA (pgRNA) secondary structure and base pairings...
November 12, 2016: World Journal of Virology
https://www.readbyqxmd.com/read/27876849/characterization-of-two-distinct-early-post-entry-blocks-to-hiv-1-in-common-marmoset-lymphocytes
#3
Beatriz Pacheco, Luis Menéndez-Arias, Joseph Sodroski
In nature, primate lentiviruses infect humans and several Old World monkeys and apes. However, to date, lentiviruses infecting New World monkeys have not been described. We studied the susceptibility of common marmoset cells to HIV-1 infection and observed the presence of post-entry blocks to the early phase of HIV-1 infection in peripheral blood lymphocytes (PBLs) and a B lymphocytic cell line (B-LCL). The blocks present in these cells are dominant and phenotypically different from each other. In PBLs, the block occurs at the level of reverse transcription, reducing the accumulation of early and late transcripts, similar to the block imposed by TRIM5α...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27862236/allogeneic-mhc-mismatched-equine-bone-marrow-derived-mesenchymal-stem-cells-are-targeted-for-death-by-cytotoxic-anti-mhc-antibodies
#4
A K Berglund, L V Schnabel
REASONS FOR PERFORMING STUDY: Allogeneic mesenchymal stem cells (MSCs) are a promising cell source for treating musculoskeletal injuries in horses. Controversy exists, however, over whether major histocompatibility complex (MHC)-mismatched MSCs are recognised by the recipient immune system and targeted for death by a cytotoxic antibody response. OBJECTIVES: To determine if cytotoxic anti-MHC antibodies generated in-vivo following MHC-mismatched MSC injections are capable of initiating complement-dependent cytotoxicity of MSCs...
November 10, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27854198/investigation-of-the-cytotoxicity-antioxidative-and-immune-modulatory-effects-of-ligusticum-porteri-osha-root-extract-on-human-peripheral-blood-lymphocytes
#5
Khanh Nguyen, Jean Sparks, Felix O Omoruyi
OBJECTIVE: Ligusticum porteri is a traditional Native American herb. The roots of L. porteri are traditionally used in the treatment of many diseases, however, its cytotoxicity, antioxidative and immune-modulatory effects need to be investigated. In this study, we evaluated the effects of the root extract at different doses on human peripheral blood lymphocytes (PBLs). METHODS: The lymphocytes were incubated with different concentrations of the root extracts (0, 50, 100, 200, and 400 μg/mL) and harvested every 6 h for 2 d (P<0...
November 2016: Journal of Integrative Medicine
https://www.readbyqxmd.com/read/27843530/effects-of-poor-hygiene-on-cytokine-phenotypes-in-children-in-the-tropics
#6
C A Figueiredo, L D Amorim, M Vaca, M E Chico, A C Campos, M L Barreto, P J Cooper
We describe immune phenotypes (innate and adaptive cytokines) according to environmental exposure using latent class analysis. A total of 310 schoolchildren living in Ecuador were assayed for spontaneous cytokine production as well as mitogen (SEB)-stimulated cytokines in whole blood cultures. We collected data on environmental exposures by questionnaire and on intestinal parasites by examination of stool samples. Latent class analysis (LCA) was used to group children according to their innate (IL-6, IL-8, IL-10 and TNF-α) and adaptive (IL-5, IL-13, IL-17, IFN-γ and IL-10) cytokine profile...
2016: World Allergy Organization Journal
https://www.readbyqxmd.com/read/27815441/soluble-rankl-cleaved-from-activated-lymphocytes-by-tnf-%C3%AE-converting-enzyme-contributes-to-osteoclastogenesis-in-periodontitis
#7
Hiroyuki Kanzaki, Seicho Makihira, Maiko Suzuki, Takenobu Ishii, Alexandru Movila, Josefine Hirschfeld, Hani Mawardi, Xiaoping Lin, Xiaozhe Han, Martin A Taubman, Toshihisa Kawai
Host immune responses play a key role in promoting bone resorption in periodontitis via receptor activator of NF-κB ligand (RANKL)-dependent osteoclastogenesis. Both membrane-bound RANKL (mRANKL) expressed on lymphocytes and soluble RANKL (sRANKL) are found in periodontal lesions. However, the underlying mechanism and cellular source of sRANKL release and its biological role in periodontitis are unclear. TNF-α-converting enzyme (TACE) is reported to cleave the following: 1) precursor TNF-α with release of mature, soluble TNF-α and 2) mRANKL with release of sRANKL...
November 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27779681/slc25a13-cdna-cloning-analysis-using-peripheral-blood-lymphocytes-facilitates-the-identification-of-a-large-deletion-mutation-molecular-diagnosis-of-an-infant-with-neonatal-intrahepatic-cholestasis-caused-by-citrin-deficiency
#8
Han-Shi Zeng, Wei-Xia Lin, Shu-Tao Zhao, Zhan-Hui Zhang, Heng-Wen Yang, Feng-Ping Chen, Yuan-Zong Song, Zhi-Nan Yin
Neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) is an autosomal recessive disorder resulting from biallelic mutations of the SLC25A13 gene. Due to the lack of well‑recognized clinical or biochemical diagnostic criteria, the definitive diagnosis of this disease relies on the genetic analysis of SLC25A13 at present. As novel large deletion/insertion mutations of the SLC25A13 gene are difficult to detect using routine DNA analytic approaches, the timely diagnosis of patients with these types of mutations remains a challenge...
October 21, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27773849/intranasal-administration-of-a-polyvalent-bacterial-lysate-induces-self-restricted-inflammation-in-the-lungs-and-a-th1-th17-memory-signature
#9
Analía Rial, Florencia Ferrara, Norma Suárez, Paola Scavone, Juan Martín Marqués, José Alejandro Chabalgoity
Respiratory tract infections are among the most frequent infections in humans causing millions of deaths especially in children and the elderly. Antibiotics and vaccines are the main available tools of control, but resistant strains are continuously arising and available vaccines only account for few of many pathogens involved. Non-specific immunotherapies are an emerging alternative to induce protective immunity at the airways. Mucosally administered polyvalent bacterial lysates (PBLs) have been widely used for decades for prevention of respiratory diseases, but the bases of their proposed therapeutic effectiveness are still controversial...
October 20, 2016: Microbes and Infection
https://www.readbyqxmd.com/read/27764774/correlation-between-anti-pd-l1-tumor-concentrations-and-tumor-specific-and-nonspecific-biomarkers-in-a-melanoma-mouse-model
#10
Ana M Contreras-Sandoval, María Merino, Marcos Vasquez, Iñaki F Trocóniz, Pedro Berraondo, María J Garrido
Blockade of PD-L1 with specific monoclonal antibodies (anti-PD-L1) represents a therapeutic strategy to increase the capability of the immune system to modulate the tumor immune-resistance. The relationship between anti-PD-L1 tumor exposition and anti-tumor effect represents a challenge that has been addressed in this work through the identification of certain biomarkers implicated in the antibody's mechanism of action, using a syngeneic melanoma mouse model. The development of an in-vitro/in-vivo platform has allowed us to investigate the PD-L1 behavior after its blockage with anti-PD-L1 at cellular level and in animals...
October 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27748796/detection-of-cancer-specific-mutations-in-early-stage-non-small-cell-lung-cancer-using-cell-free-dna-by-targeted-sequencing
#11
Yutong He, Xinyuan Zhang, Liqun Wang, Ziqiang Tian, Qingyi Liu, Jifang Yao, Yueping Liu, Chuanbao Li, Li Min, Baoen Shan
Non-small cell lung cancer (NSCLC) is a major public health problem worldwide and leads to a high mortality. NSCLC is always diagnosed in late stages because of its unapparent symptoms. However, cell-free DNA (cfDNA) may serve as a new potential biomarker to detect early stage of non‑small cell lung cancer. Here we recruited 10 non-small cell lung cancer patients to obtain fresh tumor tissue, peripheral blood lymphocytes (PBLs), and plasma. CfDNAs from 13 elderly people and 7 middle-age smokers were also extracted as controls...
October 12, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27732956/the-prognostic-value-of-dna-damage-level-in-peripheral-blood-lymphocytes-of-chemotherapy-na%C3%A3-ve-patients-with-germ-cell-cancer
#12
Zuzana Sestakova, Katarina Kalavska, Lenka Hurbanova, Dana Jurkovicova, Jan Gursky, Michal Chovanec, Daniela Svetlovska, Vera Miskovska, Jana Obertova, Patrik Palacka, Katarina Rejlekova, Zuzana Sycova-Mila, Silvia Cingelova, Stanislav Spanik, Jozef Mardiak, Miroslav Chovanec, Michal Mego
Germ cell tumors (GCTs) are extraordinarily sensitive to cisplatin (CDDP)-based chemotherapy. DNA damage represents one of the most important factors contributing to toxic effects of CDDP-based chemotherapy. This study was aimed to evaluate the prognostic value of DNA damage level in peripheral blood lymphocytes (PBLs) from chemo-naïve GCT patients. PBLs isolated from 59 chemotherapy-naïve GCT patients were included into this prospective study. DNA damage levels in PBLs were evaluated by the Comet assay and scored as percentage tail DNA by the Metafer-MetaCyte analyzing software...
October 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27729274/identification-of-a-fish-specific-chemokine-cxcl_f2-in-large-yellow-croaker-larimichthys-crocea-reveals-its-primitive-chemotactic-function
#13
Shimin Zhou, Yinnan Mu, Yingdi Liu, Jingqun Ao, Xinhua Chen
Chemokines are a superfamily of cytokines regulating immune cell migration under both inflammatory and normal physiological conditions. Currently, a number of fish specific CXC chemokines, named as CXCL_F1-5, have been identified in several species. However, understanding of their functional characteristics is still limited. In this study, we identified a fish specific chemokine CXCL_F2 (LycCXCL_F2) from large yellow croaker (Larimichthys crocea). The open reading frame (ORF) of LycCXCL_F2 is 348 nucleotides long, encoding a protein of 115 amino acids (aa)...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27685703/aberrant-methylation-of-nucleotide-excision-repair-genes-is-associated-with-chronic-arsenic-poisoning
#14
Aihua Zhang, Huiyao Li, Yun Xiao, Liping Chen, Xiaonian Zhu, Jun Li, Lu Ma, Xueli Pan, Wen Chen, Zhini He
OBJECTIVE: To define whether aberrant methylation of DNA repair genes is associated with chronic arsenic poisoning. METHODS: Hundred and two endemic arsenicosis patients and 36 healthy subjects were recruited. Methylight and bisulfite sequencing (BSP) assays were used to examine the methylation status of ERCC1, ERCC2 and XPC genes in peripheral blood lymphocytes (PBLs) and skin lesions of arsenicosis patients and NaAsO2-treated HaCaT cells. RESULTS: Hypermethylation of ERCC1 and ERCC2 and suppressed gene expression were found in PBLs and skin lesions of arsenicosis patients and was correlated with the level of arsenic exposure...
August 12, 2016: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/27685449/do-chromosome-changes-in-blood-cells-implicate-formaldehyde-as-a-leukemogen
#15
Richard J Albertini, Debra A Kaden
Formaldehyde (FA) is a mutagenic chemical - a property mitigated in vivo by rapid detoxification and limited tissue distribution following inhalation of the free agent. Endogenously produced FA is necessary for life and required for one-carbon transfer reactions; however, FA derived from external sources (exogenous FA), which may be in the form of methanol, may increase in vivo concentrations above naturally occurring physiological levels. Both endogenous and exogenous FA produce DNA monoadducts, DNA-DNA and DNA-protein cross-links (DDX and DPX) but, when exposed to exogenously-derived free FA, DNA monoadducts, DDX, and DPX are only produced at initial sites of contact...
August 11, 2016: Critical Reviews in Toxicology
https://www.readbyqxmd.com/read/27680891/macrophages-of-genetically-characterized-familial-hypercholesterolaemia-patients-show-up-regulation-of-ldl-receptor-related-proteins
#16
Rafael Escate, Teresa Padro, Maria Borrell-Pages, Rosa Suades, Rosa Aledo, Pedro Mata, Lina Badimon
Familial hypercholesterolaemia (FH) is a major risk for premature coronary heart disease due to severe long-life exposure to high LDL levels. Accumulation of LDL in the vascular wall triggers atherosclerosis with activation of the innate immunity system. Here, we have investigated (i) gene expression of LDLR and LRPs in peripheral blood cells (PBLs) and in differentiated macrophages of young FH-patients; and (ii) whether macrophage from FH patients have a differential response when exposed to high levels of atherogenic LDL...
September 29, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27663375/bovine-p-selectin-mediates-leukocyte-adhesion-and-is-highly-polymorphic-in-dairy-breeds
#17
Xing Chen, Zhangrui Cheng, Dirk Werling, Geoffrey E Pollott, Mazdak Salavati, Kate F Johnson, Faheem Ahmed Khan, D Claire Wathes, Shujun Zhang
Bovine P-selectin (SELP) mediates leukocyte rolling and primes leukocyte adhesion to endothelium, both essential for leukocyte recruitment to an infection site. We investigated SELP-mediated adhesion between bovine peripheral blood leukocytes (PBLs) and cultured bovine aortic endothelial cells pre-activated with lipopolysaccharide (LPS). We examined gene polymorphism for bovine selectins SELP, l-selectin (SELL) and E-selectin (SELE) and compared their SNP frequency between five dairy breeds (Holstein, Friesian, Jersey, Ayrshire and Brown Swiss)...
October 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27590419/non-specific-activation-of-cd8%C3%AE-characterised-%C3%AE-%C3%AE-t-cells-in-pbl-cultures-of-different-chicken-lines
#18
Christina Polasky, Steffen Weigend, Lars Schrader, Angela Berndt
Avian γδ T lymphocytes still represent an enigmatic cell population not only regarding their functions but also their activation requirements and subset differentiation. To find a suitable method for non-specific stimulation and multiplication of CD8α-characterised γδ T-cell subsets in peripheral blood lymphocyte (PBL) cultures, PBLs of four different chicken lines (WLA, BLA, R11, L68) were treated with a range of commonly used non-specific reagents (PMA, PHA, ConA) and interleukins (IL-2, IL-12, IL-15), and the CD8α(-), CD8α(hi)β(+) and CD8αα(hi+) γδ T-cell subsets examined in relation to activation (CD25 expression) and proliferation by flow cytometry...
October 15, 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27584834/time-lapse-to-colorectal-cancer-telomere-dynamics-define-the-malignant-potential-of-polyps
#19
Brooke R Druliner, Xiaoyang Ruan, Ruth Johnson, Diane Grill, Daniel O'Brien, Tsung-Po Lai, Shahrooz Rashtak, Donna Felmlee-Devine, Jill Washechek-Aletto, Andrei Malykh, Thomas Smyrk, Ann Oberg, Hongfang Liu, Jerry W Shay, David A Ahlquist, Lisa A Boardman
OBJECTIVE: Whereas few adenomas become cancer, most colorectal cancers arise from adenomas. Telomere length is a recognized biomarker in multiple cancers, and telomere maintenance mechanisms (TMM) are exploited by malignant cells. We sought to determine whether telomere length and TMM distinguish cancer-associated adenomas from those that are cancer-free. METHODS: Tissues were identified as cancer-adjacent polyp (CAP)-residual adenoma contiguous with cancer-and cancer-free polyp (CFP)-adenomas without malignancy...
2016: Clinical and Translational Gastroenterology
https://www.readbyqxmd.com/read/27580218/decreased-expression-of-programmed-death-1-on-peripheral-blood-lymphocytes-disrupts-immune-homeostasis-in-peripartum-cardiomyopathy
#20
Guozhi Xia, Xin Sun, Xiaopu Zheng, Junkui Wang
Peripartum cardiomyopathy (PPCM) is a disease of unknown pathogenesis. Programmed death 1 (PD1) has been postulated to modulate immune response through potential mechanisms that remain elusive. This study aimed to elaborate the expression and function of PD1 on peripheral blood lymphocytes (PBLs) in the development of PPCM. Specimens of PBLs were performed to determine the expression of PD1 mRNA using fluorescence quantitative RT-PCR, and Th cytokines by ELISA. Immune homeostasis was evaluated with T lymphocyte phenotypes and immunoglobulin (Ig) isotypes as well as complement factors (C)...
November 15, 2016: International Journal of Cardiology
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