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https://www.readbyqxmd.com/read/28904691/il-21-augments-rapamycin-in-expansion-of-alpha-fetoprotein-antigen-specific-stem-cell-like-memory-t-cells-in-vitro
#1
Victor Tunje Jeza, Xiaoyi Li, Jun Chen, Zhihui Liang, Adem Onago Aggrey, Xiongwen Wu
INTRODUCTION: Alloreactive tumor specific T cells are important arsenals of the adaptive immune system in the fight against tumors. However, stem cell-like memory T cells (Tscm) provide the key to effective elimination of tumor cells. Methods for generating these T cell subsets already exist. However, they could be made more efficient. Further, they are expensive and unattainable to the resource poor laboratories. In this regard, we are hereby describing a novel in vitro allogeneic co-culture method for raising allo-restricted tumor specific Tscm cells that we developed...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28862348/immunophenotype-of-peripheral-blood-lymphocytes-in-dogs-with-inflammatory-bowel-disease
#2
A Galler, B C Rütgen, E Haas, A Saalmüller, R A Hirt, W Gerner, I Schwendenwein, B Richter, J G Thalhammer, N Luckschander-Zeller
BACKGROUND: Inflammatory bowel disease (IBD) is common in dogs. Despite the known importance of intestinal lymphocytes in its pathogenesis, little is known about the role of peripheral blood lymphocytes (PBLs) in IBD. OBJECTIVES: The aims of this study were (1) comparison of PBLs analyzed by flow cytometry (FCM) in IBD dogs and healthy controls and (2) comparison of PBLs in IBD dogs at the time of diagnosis and in dogs in clinical remission. ANIMALS: Whole blood samples of 19 IBD dogs at the time of diagnosis and blood samples of 6 dogs in clinical remission were collected...
September 1, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28855224/dha-rich-n-3-fatty-acid-supplementation-decreases-dna-methylation-in-blood-leukocytes-the-omegad-study
#3
Mohsen Karimi, Inger Vedin, Yvonne Freund Levi, Hans Basun, Gerd Faxén Irving, Maria Eriksdotter, Lars-Olof Wahlund, Marianne Schultzberg, Erik Hjorth, Tommy Cederholm, Jan Palmblad
Background: Dietary fish oils, rich in long-chain n-3 (ω-3) fatty acids (FAs) [e.g., docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3)], modulate inflammatory reactions through various mechanisms, including gene expression, which is measured as messenger RNA concentration. However, the effects of long-term treatment of humans with DHA and EPA on various epigenetic factors-such as DNA methylation, which controls messenger RNA generation-are poorly described.Objective: We wanted to determine the effects of 6 mo of dietary supplementation with an n-3 FA preparation rich in DHA on global DNA methylation of peripheral blood leukocytes (PBLs) and the relation to plasma EPA and DHA concentrations in Alzheimer disease (AD) patients...
August 30, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28743632/comparative-analysis-of-dual-specificity-protein-phosphatase-genes-1-2-and-5-in-response-to-immune-challenges-in-japanese-flounder-paralichthys-olivaceus
#4
Shuo Li, Gaixiang Hao, Jiafang Li, Weijiao Peng, Xuyun Geng, Jinsheng Sun
Dual-specificity MAP kinase (MAPK) phosphatases (DUSPs) are well-established negative modulators in regulating MAPK signaling in mammalian cells and tissues. Our previous studies have shown the involvement of DUSP6 in regulating innate immunity in Japanese flounder Paralichthys olivaceus. In order to gain a better understanding of the role of DUSPs in fish innate immunity, in the present study we identified and characterized three additional DUSP genes including DUSP1, 2 and 5 in P. olivaceus. The three Japanese flounder DUSP proteins share common domain structures composed of a conserved N-terminal Rhodanase/CDC25 domain and a C-terminal catalytic phosphatase domain, while they show only less than 26% sequence identities, indicating that they may have different substrate selectivity...
September 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28727822/dna-methylation-of-candidate-genes-in-peripheral-blood-from-patients-with-type-2-diabetes-or-the-metabolic-syndrome
#5
Sanne D van Otterdijk, Alexandra M Binder, Katarzyna Szarc Vel Szic, Julia Schwald, Karin B Michels
INTRODUCTION: The prevalence of type 2 diabetes (T2D) and the metabolic syndrome (MetS) is increasing and several studies suggested an involvement of DNA methylation in the development of these metabolic diseases. This study was designed to investigate if differential DNA methylation in blood can function as a biomarker for T2D and/or MetS. METHODS: Pyrosequencing analyses were performed for the candidate genes KCNJ11, PPARγ, PDK4, KCNQ1, SCD1, PDX1, FTO and PEG3 in peripheral blood leukocytes (PBLs) from 25 patients diagnosed with only T2D, 9 patients diagnosed with T2D and MetS and 11 control subjects without any metabolic disorders...
2017: PloS One
https://www.readbyqxmd.com/read/28698119/stress-differentially-affects-the-systemic-and-leukocyte-estrogen-network-in-common-carp
#6
Ewa Szwejser, Lukasz Pijanowski, Magdalena Maciuszek, Anna Ptak, Kamil Wartalski, Malgorzata Duda, Helmut Segner, B M Lidy Verburg-van Kemenade, Magdalena Chadzinska
Both systemic and locally released steroid hormones, such as cortisol and estrogens, show immunomodulatory actions. This research gives evidence that circulating and leukocyte-derived estrogens can be involved in the regulation of the immune response in common carp, during homeostasis and upon restraining stress. It was found that stress reduced level of blood 17β-estradiol (E2) and down-regulated the gene expression of components of the "classical" estrogen system: the nuclear estrogen receptors and the aromatase CYP19, in the hypothalamus, the pituitary and in the ovaries...
July 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28688764/relt-family-members-activate-p38-and-induce-apoptosis-by-a-mechanism-distinct-from-tnfr1
#7
Pachai Moua, Mathew Checketts, Liang-Guo Xu, Hong-Bing Shu, Mary E Reyland, John K Cusick
Receptor Expressed in Lymphoid Tissues (RELT) is a human Tumor Necrosis Factor Receptor (TNFR) family member that has two identified homologous binding partners, RELL1 and RELL2. This study sought to further understand the pattern of RELT expression, the functional role of RELT family members, and the mechanism of RELT-induced apoptosis. RELT protein expression was detected in the spleen, lymph node, brain, breast and peripheral blood leukocytes (PBLs). A smaller than expected size of RELT was observed in PBLs, suggesting a proteolytically cleaved form of RELT...
September 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28630015/characterization-of-japanese-flounder-paralichthys-olivaceus-caspase1-involved-in-extracellular-atp-mediated-immune-signaling-in%C3%A2-fish
#8
Shuo Li, Weijiao Peng, Jiafang Li, Gaixiang Hao, Xuyun Geng, Jinsheng Sun
Caspase1 is a member of inflammatory Caspases that play important roles in the innate immune system. Although several teleost caspase1 genes have been identified, their partner proteins and implication in extracellular ATP-mediated immune signaling in fish are still very limited. Here we identified and characterized a caspase1 gene, named JfCaspase1, from Japanese flounder Paralichthys olivaceus. JfCaspase1 mRNA was constitutively expressed in all examined normal tissues with high expression in skin and gills and moderate expression in the enriched Japanese flounder head kidney macrophages (HKMs) and peripheral blood leukocytes (PBLs)...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28626752/protective-effect-of-a-polyherbal-aqueous-extract-comprised-of-nigella-sativa-seeds-hemidesmus-indicus-roots-and-smilax-glabra-rhizome-on-bleomycin-induced-cytogenetic-damage-in-human-lymphocytes
#9
Bandula Prasanna Galhena, S S R Samarakoon, Myrtle Ira Thabrew, Solomon F D Paul, Venkatachalam Perumal, Chinnadurai Mani
This study was carried out to determine the chemoprotective potential of a polyherbal aqueous decoction comprised of Nigella sativa (seeds), Hemidesmus indicus (roots), and Smilax glabra (rhizome) against bleomycin induced cytogenetic damage in human lymphocytes. Isolated peripheral blood lymphocytes (PBLs) were exposed to bleomycin at a dose of 40 µg/mL for 2 hrs in the presence or absence of different doses of the decoction (100, 300, and 600 µg/mL). Modulatory effect of the decoction on bleomycin induced cytogenetic damage was evaluated by (a) degree of chromosomal aberrations (CA), (b) formation of micronuclei (MN), and (c) induction of γH2AX foci in lymphocytes exposed to bleomycin...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28610607/resveratrol-distinctively-modulates-the-inflammatory-profiles-of-immune-and-endothelial-cells
#10
Joseph Schwager, Nathalie Richard, Franziska Widmer, Daniel Raederstorff
BACKGROUND: The phenolic substance resveratrol (RES) is a plant metabolite known to modulate numerous physiological functions and to exert beneficial effects as a cancer-chemopreventing agent and on neurological, hepatic, and cardiovascular systems. Since the compound affects the lifespan of yeast and flies it might be an anti-aging substance. Mechanistically, RES is involved in down regulating the inflammatory response. The pleiotropic effects of RES in cells of the immune and endothelial system were examined in this study...
June 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28592251/distinct-expression-profile-of-hcmv-encoded-mirnas-in-plasma-from-oral-lichen-planus-patients
#11
Meng Ding, Xiang Wang, Cheng Wang, Xiaoshuang Liu, Ke Zen, Wenmei Wang, Chen-Yu Zhang, Chunni Zhang
BACKGROUND: Oral lichen planus (OLP) is a T cell-mediated autoimmune disease. The aetiology and molecular mechanisms of OLP remain unclear. Human cytomegalovirus (HCMV) infection is a causal factor in the development of various diseases, but the clinical relevance of HCMV in OLP has not been thoroughly investigated. METHODS: In the present study, we firstly examined twenty-three HCMV-encoded microRNA (miRNA) expression profiles in plasma from training set that including 21 OLP patients and 18 healthy controls using RT-qPCR technology...
June 7, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28526681/adoptive-transfer-of-phosphoantigen-specific-%C3%AE-%C3%AE-t-cell-subset-attenuates-mycobacterium-tuberculosis-infection-in-nonhuman-primates
#12
Arwa Qaqish, Dan Huang, Crystal Y Chen, Zhuoran Zhang, Richard Wang, Shengpu Li, Enzhuoa Yang, Yang Lu, Michelle H Larsen, William R Jacobs, Lixia Qian, James Frencher, Ling Shen, Zheng W Chen
The dominant Vγ2Vδ2 T cell subset recognizes phosphoantigen and exists only in humans and nonhuman primates. Despite the discovery of γδ T cells >30 y ago, a proof-of-concept study has not been done to prove the principle that the Vγ2Vδ2 T cell subset is protective against Mycobacterium tuberculosis and other infections. In this study, we used an adoptive cell-transfer strategy to define the protective role of Vγ2Vδ2 T cells in a primate tuberculosis (TB) model. Vγ2Vδ2 T cells for adoptive transfer displayed central/effector memory and mounted effector functions, including the production of anti-M...
June 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28526442/comparative-study-of-interleukin-17c-il-17c-and-il-17d-in-large-yellow-croaker-larimichthys-crocea-reveals-their-similar-but-differential-functional-activity
#13
Yang Ding, Jingqun Ao, Xinhua Chen
Interleukin 17 (IL-17) family members are key players in regulating the immune response in mammals. Here, we identified the IL-17C and IL-17D homologs from large yellow croaker (Larimichthys crocea), named LcIL-17C and LcIL-17D, respectively. The deduced LcIL-17C and LcIL-17D proteins possessed the typical IL-17 domain and shared a conserved arrangement of eight cysteine residues. Both LcIL-17C and LcIL-17Dc genes were constitutively expressed in all tissues examined, although at different levels. After challenge with Aeromonas hydrophila, the expression of LcIL-17C and LcIL-17D was significantly increased in gills, head kidney, and spleen...
May 16, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28510806/role-of-core-needle-biopsy-in-primary-breast-lymphoma
#14
Giulia Bicchierai, Luigi Rigacci, Vittorio Miele, Icro Meattini, Diego De Benedetto, Valeria Selvi, Simonetta Bianchi, Lorenzo Livi, Jacopo Nori
Primary breast lymphoma (PBL) may mimic carcinoma clinically and also mammographic and ecographic distinction is subtle. Despite its rarity, incidence of PBLs has increased over the last four decades and continues to increase for younger women and for some subtypes, and for this reason it is increasingly important to achieve a preoperative pathological diagnosis using core needle biopsy (CNB) or fine-needle aspiration cytology (FNA). The aim of this retrospective study was to report our single-center experience in CNBs performed for histological diagnosis of PBL compared to FNA...
May 16, 2017: La Radiologia Medica
https://www.readbyqxmd.com/read/28432527/effects-of-agaricus-blazei-murill-polysaccharide-on-cadmium-poisoning-on-the-mda5-signaling-pathway-and-antioxidant-function-of-chicken-peripheral-blood-lymphocytes
#15
Ai Lv, Ming Ge, Xuequan Hu, Wenjing Liu, Guangxing Li, Ruili Zhang
This experimental study investigated the effect of Agaricus blazei Murill polysaccharide (ABP) on cadmium (Cd) poisoning on the melanoma differentiation-associated gene 5 (MDA5) signaling pathway and antioxidant function of peripheral blood lymphocytes (PBLs) in chickens. The experiments were divided into four groups: 7-day-old chickens with normal saline (0.2 mL single/day), Cd (140 mg/kg), ABP (30 mg/mL, 0.2 mL single/day), and Cd + ABP(140 mg/kg/day + 0.2 mL ABP). Peripheral blood was collected on the 20th, 40th, and 60th days for each group, and PBLs were separated...
April 21, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28408386/increased-pd-1-and-tim-3-tils-during-cetuximab-therapy-inversely-correlate-with-response-in-head-and-neck-cancer-patients
#16
Hyun-Bae Jie, Raghvendra M Srivastava, Athanassios Argiris, Julie E Bauman, Lawrence P Kane, Robert L Ferris
Despite emerging appreciation for the important role of immune checkpoint receptors in regulating the effector functions of T cells, it is unknown whether their expression is involved in determining the clinical outcome in response to cetuximab therapy. We examined the expression patterns of immune checkpoint receptors (including PD-1, CTLA-4, and TIM-3) and cytolytic molecules (including granzyme B and perforin) of CD8(+) tumor-infiltrating lymphocytes (TIL) and compared them with those of peripheral blood T lymphocytes (PBL) in patients with head and neck cancer (HNSCC) during cetuximab therapy...
May 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/28408239/protective-responses-of-two-paralogs-of-granulocyte-colony-stimulating-factor-gcsf-in-rock-bream-oplegnathus-fasciatus-during-bacterial-and-viral-infection
#17
Joseph Jeswin, Ji-Min Jeong, Jae-Dong Shim, Jin-Sol Bae, Chan-Il Park
Granulocyte colony stimulating factor (GCSF) has a key role in the production of neutrophilic granulocytes during normal hematopoietic development and release of neutrophils into the blood circulation. In this study we have identified and characterized two paralogs of GCSF (RbGCSF) in rock bream. Although RbGCSF-1 and RbGCSF-2 share low sequence conservation, its domains and protein structure still share significant similarity. Basal levels of RbGCSF-1 gene expression was high in peripheral blood leukocytes (PBLs), spleen and intestine whereas the RbGCSF-2 was highly expressed in PBLs and kidney, of healthy animals...
April 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28399576/local-tumor-control-and-dna-pk-activity-of-peripheral-blood-lymphocytes-in-prostate-cancer-patients-receiving-radiotherapy
#18
Masanori Someya, Tomokazu Hasegawa, Masakazu Hori, Yoshihisa Matsumoto, Kensei Nakata, Naoya Masumori, Koh-Ichi Sakata
Repair of DNA damage is critical for genomic stability, and DNA-dependent protein kinase (DNA-PK) has an important role in repairing double-strand breaks. We examined whether the DNA-PK activity of peripheral blood lymphocytes (PBLs) was related to biochemical (prostate-specific antigen: PSA) relapse and radiation toxicity in prostate cancer patients who have received radiotherapy. A total of 69 patients with localized adenocarcinoma of the prostate participated in this study. Peripheral blood was collected 2 years or later after radiotherapy and centrifuged, then DNA-PK activity was measured by a filter binding assay...
March 1, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28377270/expression-and-functional-characterization-of-vitronectin-gene-from-japanese-flounder-paralichthys-olivaceus
#19
Shuo Li, Gaixiang Hao, Weijiao Peng, Xuyun Geng, Jinsheng Sun
Vitronectin (Vtn) is a multifunctional protein that plays significant roles in cell adhesion, migration, spreading and survival, and in the regulation of membrane attack complex formation and the terminal pathway of complement activation in innate immune response. However, the expression and immune significance of Vtn in fish remains largely unknown. In order to understand the involvement of Vtn in fish innate immune response, here we cloned and characterized a full-length Vtn ortholog cDNA, termed PoVtn, from Japanese flounder Paralichthys olivaceus...
April 1, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28374070/knockdown-of-microrna-17-5p-ameliorates-atherosclerotic-lesions-in-apoe-mice-and-restores-the-expression-of-very-low-density-lipoprotein-receptor
#20
Lili Tan, Liang Meng, Xiaojing Shi, Bo Yu
OBJECTIVE: To propose and verify a hypothesis that miR-17-5p knockdown may mitigate atherosclerotic lesions using atherosclerotic ApoE(-/-) mice as serum microRNA-17-5p (miR-17-5p) is elevated in patients with atherosclerosis. RESULTS: The level of miR-17-5p was higher while the level of very low density lipoprotein receptor (VLDLR), a predicted target of miR-17-5p, was lower in the peripheral blood lymphocytes (PBLs) of atherosclerosis patients as compared with control PBLs...
April 3, 2017: Biotechnology Letters
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