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https://www.readbyqxmd.com/read/28213321/effects-of-selenium-lead-interaction-on-the-gene-expression-of-inflammatory-factors-and-selenoproteins-in-chicken-neutrophils
#1
Xiaojing Li, Mengyuan Xing, Menghao Chen, Jinxin Zhao, Ruifeng Fan, Xia Zhao, Changyu Cao, Jie Yang, Ziwei Zhang, Shiwen Xu
Lead (Pb) is one of the most highly toxic metal pollutant that can cause damage to the immune system. It is known that selenium (Se) can antagonize heavy metals. To explore the toxic effects of Pb poisoning on bird immune cells, as well as the alleviating effects of Se on Pb, Se supplement and/or Pb poisoning chicken models were established. One hundred and eighty Hyline 7-day-old male chickens received either Se (1mg Se per kg of diet), Pb (350mg Pb per liter water) or Se+Pb in their diet and water for 90 days...
February 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28212525/ablation-of-ferroptosis-regulator-glutathione-peroxidase-4-in-forebrain-neurons-promotes-cognitive-impairment-and-neurodegeneration
#2
William Sealy Hambright, Rene Solano Fonseca, Liuji Chen, Ren Na, Qitao Ran
Synaptic loss and neuron death are the underlying cause of neurodegenerative diseases such as Alzheimer's disease (AD); however, the modalities of cell death in those diseases remain unclear. Ferroptosis, a newly identified oxidative cell death mechanism triggered by massive lipid peroxidation, is implicated in the degeneration of neurons populations such as spinal motor neurons and midbrain neurons. Here, we investigated whether neurons in forebrain regions (cerebral cortex and hippocampus) that are severely afflicted in AD patients might be vulnerable to ferroptosis...
February 1, 2017: Redox Biology
https://www.readbyqxmd.com/read/28204974/lipid-peroxidation-dependent-cell-death-regulated-by-gpx4-and-ferroptosis
#3
Hirotaka Imai, Masaki Matsuoka, Takeshi Kumagai, Taro Sakamoto, Tomoko Koumura
Glutathione peroxidase 4 (Phospholipid hydroperoxide glutathione peroxidase, PHGPx) can directly reduce phospholipid hydroperoxide. Depletion of GPx4 induces lipid peroxidation-dependent cell death in embryo, testis, brain, liver, heart, and photoreceptor cells of mice. Administration of vitamin E in tissue specific GPx4 KO mice restored tissue damage in testis, liver, and heart. These results indicate that suppression of phospholipid peroxidation is essential for cell survival in normal tissues in mice. Ferroptosis is an iron-dependent non-apoptotic cell death that can elicited by pharmacological inhibiting the cystine/glutamate antiporter, system Xc(-) (type I) or directly binding and loss of activity of GPx4 (Type II) in cancer cells with high level RAS-RAF-MEK pathway activity or p53 expression, but not in normal cells...
February 16, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28203523/glutathione-peroxidase-4-plays-an-important-role-in-oxidative-homeostasis-and-wound-repair-in-corneal-epithelial-cells
#4
Osamu Sakai, Takatoshi Uchida, Hirotaka Imai, Takashi Ueta
Oxidative stress is involved in the pathologies of corneal epithelial cells. However, the importance of specific antioxidant enzymes in corneal epithelial cells is not fully understood. The purpose of this study is to elucidate the role of glutathione peroxidase 4 (GPx4) in corneal epithelial cells. For in vitro experiments, an immortalized human corneal epithelial cell line was used. Cytotoxicity measured through LDH activity, lipid peroxidation immunostained for 4-hydroxynonenal, cell viability, and cell death were compared between cells transfected with either GPx4 siRNA or scrambled control siRNA...
December 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/28140330/insights-for-setting-of-nutrient-requirements-gleaned-by-comparison-of-selenium-status-biomarkers-in-turkeys-and-chickens-versus-rats-mice-and-lambs
#5
REVIEW
Roger A Sunde, Jin-Long Li, Rachel M Taylor
To gain insights into nutrient biomarkers and setting of dietary nutrient requirements, selenium biomarker levels and requirements in response to multiple graded levels of dietary selenium were compared between day-old turkeys and chickens versus weanling rats and mice and 2-d-old lambs supplemented with sodium selenite. In rodents, there was no significant effect of dietary selenium on growth, indicating that the minimum selenium requirement was <0.007 μg Se/g diet. In contrast, there was a significant effect in turkeys, chicks, and lambs, which showed selenium requirements for growth of 0...
November 2016: Advances in Nutrition
https://www.readbyqxmd.com/read/28130223/hspa5-regulates-ferroptotic-cell-death-in-cancer-cells
#6
Shan Zhu, Qiuhong Zhang, Xiaofang Sun, Herbert J Zeh, Michael T Lotze, Rui Kang, Daolin Tang
Ferroptosis is a form of regulated cell death driven by oxidative injury promoting lipid peroxidation, although detailed molecular regulators are largely unknown. Here we show that heat shock 70kDa protein 5 (HSPA5) negatively regulates ferroptosis in human pancreatic ductal adenocarcinoma (PDAC) cells. Mechanistically, activating transcription factor 4 (ATF4) resulted in the induction of HSPA5, which in turn bound glutathione peroxidase 4 (GPX4) and protected against GPX4 protein degradation and subsequent lipid peroxidation...
January 27, 2017: Cancer Research
https://www.readbyqxmd.com/read/28088013/delineating-hierarchy-of-selenotranscriptome-expression-and-their-response-to-selenium-status-in-chicken-central-nervous-system
#7
Xiu-Qing Jiang, Chang-Yu Cao, Zhao-Yang Li, Wei Li, Cong Zhang, Jia Lin, Xue-Nan Li, Jing-Long Li
Selenium (Se) incorporated in selenoproteins as selenocysteine and supports various important cellular and organismal functions. We recently reported that chicken brain exhibited high priority for Se supply and retention under conditions of dietary Se deficiency and supernutrition Li et al. (2012) . However, the selenotranscriptome expressions and their response to Se status in chicken central nervous system (CNS) are unclear. To better understand the relationship of Se homeostasis and selenoproteins expression in chicken CNS, 1day-old HyLine White chickens were fed a low Se diet (Se-L, 0...
January 4, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28063465/maternal-obesity-in-the-rat-impairs-male-offspring-aging-of-the-testicular-antioxidant-defence-system
#8
Claudia J Bautista, Guadalupe L Rodríguez-González, Angélica Morales, Consuelo Lomas-Soria, Fabiola Cruz-Pérez, Luis A Reyes-Castro, Elena Zambrano
A high-fat diet during intrauterine development predisposes offspring (F1) to phenotypic alterations, such as lipid synthesis imbalance and increased oxidative stress, causing changes in male fertility. The objective of this study was to evaluate the effects of maternal obesity during pregnancy and lactation on antioxidant enzymes in the F1 testes. Female Wistar rats (F0) were fed either a control (C, 5% fat) or an obesogenic (MO, maternal obesity, 25% fat) diet from weaning and throughout subsequent pregnancy and lactation...
January 9, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28050792/alpha-synuclein-alters-differently-gene-expression-of-sirts-parps-and-other-stress-response-proteins-implications-for-neurodegenerative-disorders
#9
J Motyl, P L Wencel, M Cieślik, R P Strosznajder, J B Strosznajder
Alpha-synuclein (ASN) is a presynaptic protein that can easily change its conformation under different types of stress. It's assumed that ASN plays an important role in the pathogenesis of Parkinson's and Alzheimer's disease. However, the molecular mechanism of ASN toxicity has not been elucidated. This study focused on the role of extracellular ASN (eASN) in regulation of transcription of sirtuins (Sirts) and DNA-bound poly(ADP-ribose) polymerases (PARPs) - proteins crucial for cells' survival/death. Our results indicate that eASN enhanced the free radicals level, decreased mitochondria membrane potential, cells viability and activated cells' death...
January 3, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28043022/spliced-leader-based-analyses-reveal-the-effects-of-polycyclic-aromatic-hydrocarbons-on-gene-expression-in-the-copepod-pseudodiaptomus-poplesia
#10
Yunyun Zhuang, Feifei Yang, Donghui Xu, Hongju Chen, Huan Zhang, Guangxing Liu
Polycyclic aromatic hydrocarbons (PAHs) are a group of toxic and carcinogenic pollutants that can adversely affect the development, growth and reproduction of marine organisms including copepods. However, knowledge on the molecular mechanisms regulating the response to PAH exposure in marine planktonic copepods is limited. In this study, we investigated the survival and gene expression of the calanoid copepod Pseudodiaptomus poplesia upon exposure to two PAHs, 1, 2-dimethylnaphthalene (1, 2-NAPH) and pyrene...
February 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28011267/methotrexate-induced-germ-cell-toxicity-and-the-important-role-of-zinc-and-sod1-investigation-of-molecular-mechanisms
#11
Krishna Prahlad Maremanda, G B Jena
Zinc (Zn) was proved to be a germ cell protectant against various disease conditions and toxic insults. Besides other mechanisms, here we have explored the important role of Zn and Zn-dependent SOD1in methotrexate (MTX)-induced germ cell damage. MTX was given 5 mg/kg i.p. once a week for four consecutive weeks, while Zn was supplemented daily at the doses of 3 and 6 mg/kg i.p. for four consecutive weeks. After four weeks of treatment the animals were sacrificed and observed for various end points. There were several histopahtological alterations in the testes like desquamation and altered tubular structures...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28007408/transcriptional-abundance-of-antioxidant-enzymes-in-endometrium-and-their-circulating-levels-in-zebu-cows-with-and-without-uterine-infection
#12
R K Baithalu, S K Singh, A Kumaresan, A K Mohanty, T K Mohanty, S Kumar, S Kerketta, B R Maharana, T K Patbandha, N Attupuram, S K Agarwal
Oxidative stress during peripartum period may compromise the uterine immunity. In the present study, we assessed the oxidative stress and antioxidant status during peripartum period and studied their relationship with postpartum uterine infection in dairy cows. Peripheral blood concentrations of total antioxidant capacity (TAC), malondialdehyde (MDA) and nitric oxide (NO) were determined (day -21, -7, on the day of calving and day +7, +21, +35) in normal (n=11), puerperal metritic (n=7) and clinical endometritic (n=6) cows...
February 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/27993104/short-term-effects-of-t-2-toxin-or-deoxynivalenol-on-lipid-peroxidation-and-the-glutathione-system-in-common-carp
#13
Csilla Pelyhe, Benjámin Kövesi, Erika Zándoki, Balázs Kovács, Judit Szabó-Fodor, Miklós Mézes, Krisztián Balogh
The purpose of this study was to investigate the short-term effects of a single oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg(-1) body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg(-1) body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours...
December 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27982062/changes-in-mitochondrial-homeostasis-and-redox-status-in-astronauts-following-long-stays-in-space
#14
Hiroko P Indo, Hideyuki J Majima, Masahiro Terada, Shigeaki Suenaga, Kazuo Tomita, Shin Yamada, Akira Higashibata, Noriaki Ishioka, Takuro Kanekura, Ikuya Nonaka, Clare L Hawkins, Michael J Davies, Daret K St Clair, Chiaki Mukai
The effects of long-term exposure to extreme space conditions on astronauts were investigated by analyzing hair samples from ten astronauts who had spent six months on the International Space Station (ISS). Two samples were collected before, during and after their stays in the ISS; hereafter, referred to as Preflight, Inflight and Postflight, respectively. The ratios of mitochondrial (mt) to nuclear (n) DNA and mtRNA to nRNA were analyzed via quantitative PCR. The combined data of Preflight, Inflight and Postflight show a significant reduction in the mtDNA/nDNA in Inflight, and significant reductions in the mtRNA/nRNA ratios in both the Inflight and Postflight samples...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27964880/role-of-gpx4-in-human-vascular-endothelial-cells-and-the-compensatory-activity-of-brown-rice-on-gpx4-ablation-condition
#15
Osamu Sakai, Toshinori Yasuzawa, Yoshie Sumikawa, Takashi Ueta, Hirotaka Imai, Akiyoshi Sawabe, Shigeru Ueshima
Oxidative stress is implicated in the pathologies of vascular endothelial cells. However, the importance of specific antioxidant enzymes in vascular endothelial cells is not fully understood. The purpose of this study was to elucidate the importance of Glutathione peroxidase 4 (GPx4), and the involvement of ferroptosis on cell death induced by GPx4 loss in human vascular endothelial cells. In addition, we examined the compensatory activity of brown rice on GPx4 ablation condition. Human umbilical vein endothelial cells were transfected with GPx4 or scramble control siRNA...
December 1, 2016: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://www.readbyqxmd.com/read/27957666/decreased-expression-of-selenoproteins-as-a-poor-prognosticator-of-gastric-cancer-in-humans
#16
Xiuwen Lan, Jun Xing, Hongyu Gao, Sen Li, Lina Quan, Yang Jiang, Shaohua Ding, Yingwei Xue
The aim of the present study was to analyze the selenoprotein expression levels in gastric cancer patients. We enrolled 40 patients (29 males, 11 females) who were recently diagnosed with gastric cancer and 50 healthy people (30 males, 20 females) as controls. The expression of 25 selenoprotein genes (Dio1, Dio2, Dio3, Gpx1, Gpx2, Gpx3, Gpx4, Gpx6, SelH, SelI, SelK, SelM, SelN, SelO, SelP, SelS, SelT, SelV, SelW, SelX, Sel15, Sps2, TR1, TR2, and TR3) in human gastric cancer tissues, para-carcinoma tissues, adjacent normal gastric tissues, erythrocytes, and lymphocytes in the gastric cancer group and healthy control group was analyzed by qRT-PCR...
December 13, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27929073/rna-sequencing-of-chorionic-villi-from-recurrent-pregnancy-loss-patients-reveals-impaired-function-of-basic-nuclear-and-cellular-machinery
#17
Siim Sõber, Kristiina Rull, Mario Reiman, Piret Ilisson, Pirkko Mattila, Maris Laan
Recurrent pregnancy loss (RPL) concerns ~3% of couples aiming at childbirth. In the current study, transcriptomes and miRNomes of 1(st) trimester placental chorionic villi were analysed for 2 RPL cases (≥6 miscarriages) and normal, but electively terminated pregnancies (ETP; n = 8). Sequencing was performed on Illumina HiSeq 2000 platform. Differential expression analyses detected 51 (27%) transcripts with increased and 138 (73%) with decreased expression in RPL compared to ETP (DESeq: FDR P < 0...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27860262/ferroptosis-a-new-form-of-cell-death-and-its-relationships-with-tumourous-diseases
#18
REVIEW
Haitao Yu, Pengyi Guo, Xiaozai Xie, Yi Wang, Gang Chen
Ferroptosis is a newly discovered type of cell death that differs from traditional apoptosis and necrosis and results from iron-dependent lipid peroxide accumulation. Ferroptotic cell death is characterized by cytological changes, including cell volume shrinkage and increased mitochondrial membrane density. Ferroptosis can be induced by two classes of small-molecule substances known as class 1 (system Xc(-) inhibitors) and class 2 ferroptosis inducers [glutathione peroxidase 4 (GPx4) inhibitors]. In addition to these small-molecule substances, a number of drugs (e...
November 10, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27836545/discovery-of-gpx4-inhibitory-peptides-from-random-peptide-t7-phage-display-and-subsequent-structural-analysis
#19
Kotaro Sakamoto, Satoshi Sogabe, Yusuke Kamada, Shin-Ichi Matsumoto, Akito Kadotani, Jun-Ichi Sakamoto, Akiyoshi Tani
The phospholipid hydroperoxidase glutathione peroxidase (GPX4) is an enzyme that reduces lipid hydroperoxides in lipid membranes. Recently, GPX4 has been investigated as a target molecule that induces iron-dependent cell death (ferroptosis) selectively in cancer cells that express mutant Ras. GPX4 inhibitors have the potential to become novel anti-cancer drugs. However, there are no druggable pockets for conventional small molecules on the molecular surface of GPX4. To generate GPX4 inhibitors, we examined the use of peptides as an alternative to small molecules...
January 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27816518/antioxidant-responses-in-hibernating-chinese-soft-shelled-turtle-pelodiscus-sinensis-hatchlings
#20
Wen-Yi Zhang, Cui-Juan Niu, Bo-Jian Chen, Lin Yuan
The antioxidant defense system protects turtles from oxidative stress during hibernation. The present study examined changes of the antioxidant enzymes both on mRNA level and enzyme activity level during hibernation of Chinese soft-shelled turtle Pelodiscus sinensis hatchlings. The upstream regulator NF-E2 related factor 2 (Nrf2) mRNA was also measured. Samples were taken at pre-hibernation (17.0°C, Mud temperature (MT)), hibernation (5.8°C, MT) and arousal (20.1°C, MT). Nrf2 exhibited a tissue-specific pattern of expression with a decrease in the brain, slight increase in the liver and heart during hibernation, and significant increase during arousal in all the three tissues...
February 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
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