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https://www.readbyqxmd.com/read/29778389/resveratrol-improves-in-vitro-maturation-of-oocytes-in-aged-mice-and-humans
#1
Mei-Ju Liu, Ai-Gang Sun, Shi-Gang Zhao, Hui Liu, Shui-Ying Ma, Mei Li, Ying-Xue Huai, Han Zhao, Hong-Bin Liu
OBJECTIVE: To evaluate the effects of resveratrol on oocyte maturation in aged mice and humans. DESIGN: Experimental laboratory study. SETTING: University-based reproductive medicine center. PATIENT(S): A total of 64 women 38-45 years of age undergoing intracytoplasmic sperm injection (ICSI) and 48-52-week-old female C57BL/6J mice. INTERVENTION(S): In vitro culture in the presence of three different concentrations of resveratrol (0...
May 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29713468/dietary-resveratrol-improves-antioxidant-status-of-sows-and-piglets-and-regulates-antioxidant-gene-expression-in-placenta-by-keap1-nrf2-pathway-and-sirt1
#2
Qingwei Meng, Tao Guo, Gaoqiang Li, Shishuai Sun, Shiqi He, Baojing Cheng, Baoming Shi, Anshan Shan
Background: Resveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects of dietary resveratrol supplementation during pregnancy and lactation on the antioxidant status of sows and piglets and on antioxidant gene expression and pathway in placenta. Methods: Forty sows were allotted to 2 dietary treatments 20 d after breeding. Sows were fed a control diet and a control diet with 300 mg/kg resveratrol...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29709707/consequences-of-mutations-and-inborn-errors-of-selenoprotein-biosynthesis-and-functions
#3
REVIEW
Noelia Fradejas-Villar
In its 200 years of history, selenium has been defined first as a toxic element and finally as a micronutrient. Selenium is incorporated into selenoproteins as selenocysteine (Sec), the 21st proteinogenic amino acid codified by a stop codon. Specific biosynthetic factors recode UGA stop codon as Sec. The significance of selenoproteins in human health is manifested through the identification of patients with inborn errors in selenoproteins or their biosynthetic factors. Selenoprotein N-related myopathy was the first disease identified due to mutations in a selenoprotein gene...
April 27, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29707428/rnai-mediated-knockdown-of-mtnr1b-without-disrupting-the-effects-of-melatonin-on-apoptosis-and-cell-cycle-in-bovine-granulose-cells
#4
Wenju Liu, Shujuan Wang, Jinxing Zhou, Xunsheng Pang, Like Wang
Melatonin is well known as a powerful free radical scavenger and exhibits the ability to prevent cell apoptosis. In the present study, we investigated the role of melatonin and its receptor MTNR1B in regulating the function of bovine granulosa cells (GCs) and hypothesized the involvement of MTNR1B in mediating the effect of melatonin on GCs. Our results showed that MTNR1B knockdown significantly promoted GCs apoptosis but did not affect the cell cycle. These results were further verified by increasing the expression of pro-apoptosis genes ( BAX and CASP3 ), decreasing expression of the anti-apoptosis genes ( BCL2 and BCL-XL ) and anti-oxidant genes ( SOD1 and GPX4 ) without affecting cell cycle factors ( CCND1 , CCNE1 and CDKN1A ) and TP53 ...
2018: PeerJ
https://www.readbyqxmd.com/read/29702237/molecular-characterization-and-functional-analysis-of-two-phospholipid-hydroperoxide-isoforms-from-larimichthys-crocea-under-vibrio-parahaemolyticus-challenge
#5
Xiaoze Xie, Mengnan Chen, Aiyi Zhu
Glutathione peroxidases family is a key role in the antioxidant system in oxybiotic organisms for cell redox homeostasis. One of their members, phospholipid hydroperoxide glutathione peroxidase (GPx4) have unique monomeric structure and can directly react with complex lipid and membrane-bound peroxides under the presence of glutathione(GSH). In this paper, two complete GPx4 cDNAs (designated as LcGPx4a and LcGPx4b) from Larimichthys crocea are identified by rapid amplification of cDNA ends. The cDNA of LcGPx4a was consisted of a 5'-untranslated region (UTR) of 258 bp, a 3'-UTR of 330 bp, and an open reading frame (ORF) of 561 bp encoding 186 amino acid (aa) polypeptides...
April 24, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29688708/novel-allosteric-activators-for-ferroptosis-regulator-glutathione-peroxidase-4
#6
Cong Li, Xiaobing Deng, Weilin Zhang, Xiaowen Xie, Marcus Conrad, Ying Liu, José Pedro Friedmann Angeli, Luhua Lai
Glutathione peroxidase 4 (GPX4) is essential for cell membrane repair, inflammation suppression, and ferroptosis inhibition. GPX4 upregulation provides unique drug discovery opportunities for inflammation and ferroptosis-related diseases. However, rational design of protein activators is challenging. Until now, no compound has been reported to activate the enzyme activity of GPX4. Here, we identified a potential allosteric site in GPX4, and successfully found eight GPX4 activators using a novel computational strategy and experimental studies...
April 24, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29673971/changes-in-trophoblasts-gene-expression-in-response-to-perchlorate-exposition
#7
De la Peña Sol, Santiago-Roque Isela, Olivo-Vidal Zendy, Navarro-Meza Mónica, Xochihua-Rosas Irene, Arroyo-Helguera Omar
Contaminated water with chlorates is a public health problem associated with iodine deficiency. Epidemiological evidence shows that iodine deficiency is a risk factor for preeclampsia (PE). In this study we use human BeWo trophoblast cells exposed to perchlorate (KClO4 ) and changes in gene expression were analyzed by microarrays, quantitative RT-PCR (qRT-PCR) and immunoblot. The microarray analysis identified 48 transcripts up-regulated and 112 down-regulated in comparison with non-exposed trophoblast. The qRT-PCR analysis confirmed changes in GAS7, PKP2, Emilin, Dynatic 3, protocadherins 11, 15, gamma A12, EGFR, SAFB1, ACE2, ANXA2, Apoliprotein E, SREBF1, and C/EBP-β...
April 16, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29610484/fino-2-initiates-ferroptosis-through-gpx4-inactivation-and-iron-oxidation
#8
Michael M Gaschler, Alexander A Andia, Hengrui Liu, Joleen M Csuka, Brisa Hurlocker, Christopher A Vaiana, Daniel W Heindel, Dylan S Zuckerman, Pieter H Bos, Eduard Reznik, Ling F Ye, Yulia Y Tyurina, Annie J Lin, Mikhail S Shchepinov, Amy Y Chan, Eveliz Peguero-Pereira, Maksim A Fomich, Jacob D Daniels, Andrei V Bekish, Vadim V Shmanai, Valerian E Kagan, Lara K Mahal, K A Woerpel, Brent R Stockwell
Ferroptosis is a non-apoptotic form of regulated cell death caused by the failure of the glutathione-dependent lipid-peroxide-scavenging network. FINO2 is an endoperoxide-containing 1,2-dioxolane that can initiate ferroptosis selectively in engineered cancer cells. We investigated the mechanism and structural features necessary for ferroptosis initiation by FINO2 . We found that FINO2 requires both an endoperoxide moiety and a nearby hydroxyl head group to initiate ferroptosis. In contrast to previously described ferroptosis inducers, FINO2 does not inhibit system xc - or directly target the reducing enzyme GPX4, as do erastin and RSL3, respectively, nor does it deplete GPX4 protein, as does FIN56...
April 2, 2018: Nature Chemical Biology
https://www.readbyqxmd.com/read/29609868/genetic-variants-in-selenoprotein-genes-modulate-biomarkers-of-selenium-status-in-response-to-brazil-nut-supplementation-the-su-bra-nut-study
#9
Janaina L S Donadio, Marcelo M Rogero, Elvira M Guerra-Shinohara, Fernando Barbosa, Charles Desmarchelier, Patrick Borel, Alan A Sneddon, John E Hesketh, Silvia M F Cozzolino
BACKGROUND: The beneficial effects of selenium (Se) to human health are exerted by selenoproteins, which can be quantified in blood and used as biomarkers of Se status. Different responses of Se biomarkers after supplementation with selenomethionine and sodium selenite have been observed and some of them could be due to genetic polymorphisms, mainly single nucleotide polymorphisms (SNPs). Brazil nuts are known to be the richest natural source of Se. OBJECTIVE: Investigate how genetic variations in selenoprotein genes modulate biomarkers of Se status in response to Brazil nut supplementation...
March 23, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29604131/changes-in-the-regulation-and-activity-of-glutathione-redox-system-and-lipid-peroxidation-processes-in-short-term-aflatoxin-b1-exposure-in-liver-of-laying-hens
#10
M Erdélyi, K Balogh, C Pelyhe, B Kövesi, M Nakade, E Zándoki, M Mézes, B Kovács
The purpose of this study was to investigate the short-term (48 hr) effects of feeding aflatoxin contaminated diet (170.3 μg/kg AFB1) in 49-week-old laying hens. Liver samples were taken at 12-hr intervals. Feed intake, body weight, absolute and relative liver weight were the same in groups. However, there was no feed intake during both dark periods (between 12nd to 24th and 36th to 48th hours of the experiment); therefore, aflatoxin intake was also negligible. Markers of initial phase of lipid peroxidation, conjugated dienes and trienes did not change as effect of aflatoxin, but terminal marker, malondialdehyde content was significantly higher at 12 hr as effect of aflatoxin...
March 31, 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29595330/neuroprotective-effects-of-psalmotoxin-1-an-acid-sensing-ion-channel-asic-inhibitor-in-ischemia-reperfusion-in-mouse-eyes
#11
Adnan Dibas, Cameron Millar, Abraham Al-Farra, Thomas Yorio
PURPOSE: The purpose of the current study is to assess changes in the expression of Acid-Sensing Ion Channel (ASIC)1a and ASIC2 in retinal ganglion cells (RGCs) after retinal ischemia and reperfusion (I/R) injury and to test if inhibition of ASIC1a provides RGC neuroprotection. METHODS: Transient ischemia was induced in one eye of C57BL/6 mice by raising intraocular pressure to 120 mmHg for 60 min followed by retinal reperfusion by restoring normal pressure. RGC function was measured by Pattern electroretinography (PERG)...
March 29, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29588180/selenium-versus-sulfur-reversibility-of-chemical-reactions-and-resistance-to-permanent-oxidation-in-proteins-and-nucleic-acids
#12
REVIEW
Michael J Maroney, Robert J Hondal
This review highlights the contributions of Jean Chaudière to the field of selenium biochemistry. Chaudière was the first to recognize that one of the main reasons that selenium in the form of selenocysteine is used in proteins is due to the fact that it strongly resists permanent oxidation. The foundations for this important concept was laid down by Al Tappel in the 1960's and even before by others. The concept of oxygen tolerance first recognized in the study of glutathione peroxidase was further advanced and refined by those studying [NiFeSe]-hydrogenases, selenosubtilisin, and thioredoxin reductase...
March 24, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29584411/targeting-dependency-on-the-gpx4-lipid-peroxide-repair-pathway-for-cancer-therapy
#13
Hengrui Liu, Stuart L Schreiber, Brent R Stockwell
No abstract text is available yet for this article.
March 27, 2018: Biochemistry
https://www.readbyqxmd.com/read/29580086/short-term-effects-of-t-2-toxin-or-deoxynivalenol-on-glutathione-status-and-expression-of-its-regulatory-genes-in-chicken
#14
Mangesh Nakade, Csilla Pelyhe, Benjámin Kövesi, Krisztián Balogh, Balázs Kovács, Judit Szabó-Fodor, Erika Zándoki, Miklós Mézes, Márta Erdélyi
Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48...
March 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29551679/enpp2-protects-cardiomyocytes-from-erastin-induced-ferroptosis
#15
Yu-Ting Bai, Rong Chang, Hua Wang, Feng-Jun Xiao, Ri-Li Ge, Li-Sheng Wang
Ferroptosis is an iron- and oxidative-dependent form of regulated cell death and may play important roles in maintaining myocardium homeostasis and pathology of cardiovascular diseases. Currently, the regulatory roles of lipid signals in regulating cardiomyocytes ferroptosis has not been explored. In this study, we show that ENPP2, as a lipid kinase involved in lipid metabolism, protects against erastin-induced ferroptosis in cardiomyocytes. The classical ferroptosis inducer erastin remarkably inhibits the growth which could be rescued by the small molecule Fer-1 in H9c2 cells...
March 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29531187/effects-of-epigallocatechin-3-gallate-on-bovine-oocytes-matured-in-vitro
#16
Ziqiang Huang, Yunwei Pang, Haisheng Hao, Weihua Du, Xueming Zhao, Huabin Zhu
Objective: Epigallocatechin-3-gallate (EGCG) is a major ingredient of catechin polyphenols and is considered one of the most promising bioactive compounds in green tea because of its strong antioxidant properties. However, the protective role of EGCG in bovine oocyte in vitro maturation (IVM) has not been investigated. Therefore, we aimed to study the effects of EGCG on IVM of bovine oocytes. Methods: Bovine oocytes were treated with different concentrations of EGCG (0, 25, 50 100, and 200 μM) , and the nuclear and cytoplasmic maturation, cumulus cell expansion, intracellular reactive oxygen species (ROS) levels, total antioxidant capacity, the early apoptosis and the developmental competence of in vitro fertilized embryos were measured...
March 13, 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/29522794/selenium-and-gpx4-a-vital-symbiosis
#17
REVIEW
José Pedro Friedmann Angeli, Marcus Conrad
Selenium has transitioned from an environmental poison and carcinogen to an essential micronutrient associated with a broad array of health promoting effects. These beneficial effects are now accepted to be linked to its incorporation into selenoproteins, a family of rare proteins utilizing a specialized translation machinery to integrate selenium in the form of selenocysteine. Despite this recognized role, much less is known regarding the actual role of selenium in these proteins. Here, we will provide the reader with an overview of the essential role of specific selenoproteins and their link to pathology based on mouse studies and relevant mutations discovered in humans...
March 6, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29515790/impact-of-glutathione-peroxidase-4-on-cell-proliferation-angiogenesis-and-cytokine-production-in-hepatocellular-carcinoma
#18
Nataliya Rohr-Udilova, Eva Bauer, Gerald Timelthaler, Robert Eferl, Klaus Stolze, Matthias Pinter, Martha Seif, Hubert Hayden, Thomas Reiberger, Rolf Schulte-Hermann, Markus Peck-Radosavljevic, Dagmar Stoiber, Michael Trauner
Insufficient supplementation with the micronutrient selenium and persistent hepatic inflammation predispose to hepatocellular carcinoma (HCC). Inflammation-associated reactive oxygen species attack membrane lipids and form lipid hydroperoxides able to propagate oxidative hepatic damage. Selenium-containing enzyme glutathione peroxidase 4 (GPx4) antagonizes this damage by reducing lipid hydroperoxides to respective hydroxides. However, the role of GPx4 in HCC remains elusive. We generated two human HCC cell lines with stable overexpression of GPx4, performed xenotransplantation into NOD...
February 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29502249/impact-of-glutathione-peroxidase-1-gpx1-genotype-on-selenoenzyme-and-transcript-expression-when-repleting-selenium-deficient-mice
#19
Roger A Sunde, Edward T Zemaitis, Andrew B Blink, Julia A Lawinger
Glutathione peroxidase (Gpx1) is the major selenoprotein in most tissues in animals. Knockout (KO) of Gpx1 decreases Gpx1 activity to near zero and substantially reduces liver selenium (Se) levels, but has no overt effects in otherwise healthy mice. To investigate the impact of deletion of Gpx1 on Se metabolism, Se flux, and apparent Se requirements, KO, Gpx1 heterozygous (Het), and Gpx1 wild-type (WT) mice were fed Se-deficient diet for 17 weeks, then repleted with graded levels of Se (0-0.3 μg Se/g as Na2 SeO3 ) for 7 days, and selenoprotein activities and transcripts were determined in blood, liver, and kidney...
March 3, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29488609/ebselen-suppresses-inflammation-induced-by-helicobacter-pylori-lipopolysaccharide-via-the-p38-mitogen-activated-protein-kinase-signaling-pathway
#20
Ling Xu, Changguo Gong, Guangming Li, Jue Wei, Ting Wang, Wenying Meng, Min Shi, Yugang Wang
Ebselen is a seleno-organic compound that has been demonstrated to have antioxidant and anti-inflammatory properties. A previous study determined that ebselen inhibits airway inflammation induced by inhalational lipopolysaccharide (LPS), however, the underlying molecular mechanism remains to be elucidated. The present study investigated the effect of ebselen on the glutathione peroxidase (GPX)‑reactive oxygen species (ROS) pathway and interleukin‑8 (IL‑8) expression induced by Helicobacter pylori LPS in gastric cancer (GC) cells...
May 2018: Molecular Medicine Reports
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