keyword
https://read.qxmd.com/read/34746147/alternative-functions-of-cell-cycle-related-and-dna-repair-proteins-in-post-mitotic-neurons
#21
JOURNAL ARTICLE
Remi Akagawa, Yo-Ichi Nabeshima, Takeshi Kawauchi
Proper regulation of neuronal morphological changes is essential for neuronal migration, maturation, synapse formation, and high-order function. Many cytoplasmic proteins involved in the regulation of neuronal microtubules and the actin cytoskeleton have been identified. In addition, some nuclear proteins have alternative functions in neurons. While cell cycle-related proteins basically control the progression of the cell cycle in the nucleus, some of them have an extra-cell cycle-regulatory function (EXCERF), such as regulating cytoskeletal organization, after exit from the cell cycle...
2021: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/34537275/routine-wastewater-based-monitoring-of-antibiotic-resistance-in-two-finnish-hospitals-focus-on-carbapenem-resistance-genes-and-genes-associated-with-bacteria-causing-hospital-acquired-infections
#22
JOURNAL ARTICLE
J Majlander, V-J Anttila, W Nurmi, A Seppälä, J Tiedje, W Muziasari
BACKGROUND: Wastewater-based monitoring represents a useful tool for antibiotic resistance surveillance. AIM: To investigate the prevalence and abundance of antibiotic resistance genes (ARGs) in hospital wastewater over time. METHODS: Wastewater from two hospitals in Finland (HUS1 and HUS2) was monitored weekly for nine weeks (weeks 25-33) in summer 2020. A high-throughput real-time polymerization chain reaction (HT-qPCR) system was used to detect and quantify 216 ARGs and genes associated with mobile genetic elements (MGEs), integrons, and bacteria causing hospital-acquired infections (HAIs), as well as the 16S rRNA gene...
November 2021: Journal of Hospital Infection
https://read.qxmd.com/read/34173014/sec61g-identified-as-a-prognostic-biomarker-of-head-and-neck-squamous-cell-carcinoma
#23
JOURNAL ARTICLE
Yanan Shi, Yibo Liang, Wei Wang, Guimin Zhang
PURPOSE: It is of obvious interest to identify clinical prognosis-related oncogenes in HNSCC (head and neck squamous cell carcinoma). METHODS: Based on the available datasets within the TCGA (The Cancer Genome Atlas) and the GEO (Gene Expression Omnibus) databases, the potential mechanism of action of the SEC61G (SEC61 translocon subunit gamma) gene in HNSCC tumorigenesis was explored by several bioinformatics approaches. RESULTS: There was a higher expression level of SEC61G in primary HNSCC tumor tissues than in normal tissues...
April 2022: European Archives of Oto-rhino-laryngology
https://read.qxmd.com/read/34142156/structure-of-the-mammalian-adenine-dna-glycosylase-mutyh-insights-into-the-base-excision-repair-pathway-and-cancer
#24
JOURNAL ARTICLE
Teruya Nakamura, Kohtaro Okabe, Shogo Hirayama, Mami Chirifu, Shinji Ikemizu, Hiroshi Morioka, Yusaku Nakabeppu, Yuriko Yamagata
Mammalian MutY homologue (MUTYH) is an adenine DNA glycosylase that excises adenine inserted opposite 8-oxoguanine (8-oxoG). The inherited variations in human MUTYH gene are known to cause MUTYH-associated polyposis (MAP), which is associated with colorectal cancer. MUTYH is involved in base excision repair (BER) with proliferating cell nuclear antigen (PCNA) in DNA replication, which is unique and critical for effective mutation-avoidance. It is also reported that MUTYH has a Zn-binding motif in a unique interdomain connector (IDC) region, which interacts with Rad9-Rad1-Hus1 complex (9-1-1) in DNA damage response, and with apurinic/apyrimidinic endonuclease 1 (APE1) in BER...
July 9, 2021: Nucleic Acids Research
https://read.qxmd.com/read/33711383/mir-340-3p-hus1-axis-suppresses-proliferation-and-migration-in-lung-adenocarcinoma-cells
#25
JOURNAL ARTICLE
Kaiming Ren, Yong Yu, Xiwen Wang, Haijun Liu, Jungang Zhao
AIMS: The functions and molecular mechanisms of miR-340-3p in lung adenocarcinoma (LUAD) progression remain unclear. On the other hand, the role of HUS1 in LUAD progression should be further explored. MAIN METHODS: Data from cancer database were subjected to bioinformatics analysis. Quantitative real-time PCR and western blot were performed to detect gene expression. Colony formation and MTT assay were performed to examine cell growth in vitro. Wound healing assays and transwell assays were performed to examine cell migration...
June 1, 2021: Life Sciences
https://read.qxmd.com/read/33424379/methanolic-extract-of-citrullus-colocynthis-suppresses-growth-and-proliferation-of-breast-cancer-cells-through-regulation-of-cell-cycle
#26
JOURNAL ARTICLE
Sadia Perveen, Hanfa Ashfaq, Saira Ambreen, Isbah Ashfaq, Zakia Kanwal, Asima Tayyeb
Breast cancer is a major cause of cancer related deaths in women worldwide. Available treatments pose serious limitations such as systemic toxicity, metastasis, tumor recurrence, off-target effects, and drug resistance. In recent years, phytochemicals such as secondary metabolites due to their effective anticancer potential at very low concentration have gained attention. Aim of the study was to evaluate anticancer potential of Citrullus colocynthis and its possible molecular targets on MCF-7, a human breast cancer cell line...
January 2021: Saudi Journal of Biological Sciences
https://read.qxmd.com/read/33137086/in-vivo-mirna-knockout-screening-identifies-mir-190b-as-a-novel-tumor-suppressor
#27
JOURNAL ARTICLE
Hui Hong, Shun Yao, Yuanyuan Zhang, Yi Ye, Cheng Li, Liang Hu, Yihua Sun, Hsin-Yi Huang, Hongbin Ji
MicroRNAs (miRNAs) play important roles in the development of various cancers including lung cancer which is one of the devastating diseases worldwide. How miRNAs function in de novo lung tumorigenesis remains largely unknown. We here developed a CRISPR/Cas9-mediated dual guide RNA (dgRNA) system to knockout miRNAs in genetically engineered mouse model (GEMM). Through bioinformatic analyses of human lung cancer miRNA database, we identified 16 downregulated miRNAs associated with malignant progression and performed individual knockout with dgRNA system in KrasG12D/Trp53L/L (KP) mouse model...
November 2020: PLoS Genetics
https://read.qxmd.com/read/32784836/s-adenosylmethionine-treatment-of-colorectal-cancer-cell-lines-alters-dna-methylation-dna-repair-and-tumor-progression-related-gene-expression
#28
JOURNAL ARTICLE
Sára Zsigrai, Alexandra Kalmár, Zsófia B Nagy, Barbara K Barták, Gábor Valcz, Krisztina A Szigeti, Orsolya Galamb, Titanilla Dankó, Anna Sebestyén, Gábor Barna, Vanessza Szabó, Orsolya Pipek, Anna Medgyes-Horváth, István Csabai, Zsolt Tulassay, Péter Igaz, István Takács, Béla Molnár
Global DNA hypomethylation is a characteristic feature of colorectal carcinoma (CRC). The tumor inhibitory effect of S-adenosylmethionine (SAM) methyl donor has been described in certain cancers including CRC. However, the molecular impact of SAM treatment on CRC cell lines with distinct genetic features has not been evaluated comprehensively. HT-29 and SW480 cells were treated with 0.5 and 1 mmol/L SAM for 48 h followed by cell proliferation measurements, whole-genome transcriptome and methylome analyses, DNA stability assessments and exome sequencing...
August 9, 2020: Cells
https://read.qxmd.com/read/32783654/succinylation-at-a-key-residue-of-fen1-is-involved-in-the-dna-damage-response-to-maintain-genome-stability
#29
JOURNAL ARTICLE
Rongyi Shi, Yiyi Wang, Ya Gao, Xiaoli Xu, Shuyu Mao, Yue Xiao, Shuang Song, Liangyan Wang, Bing Tian, Ye Zhao, Yuejin Hua, Hong Xu
Human flap endonuclease 1 (FEN1) is a structure-specific, multifunctional endonuclease essential for DNA replication and repair. Our previous study showed that in response to DNA damage, FEN1 interacts with the PCNA-like Rad9-Rad1-Hus1 complex instead of PCNA to engage in DNA repair activities, such as stalled DNA replication fork repair, and undergoes SUMOylation by SUMO-1. Here, we report that succinylation of FEN1 was stimulated in response to DNA replication fork-stalling agents, such as ultraviolet (UV) irradiation, hydroxyurea, camptothecin, and mitomycin C...
October 1, 2020: American Journal of Physiology. Cell Physiology
https://read.qxmd.com/read/32686935/transcriptional-changes-in-the-ovaries-of-perch-from-chernobyl
#30
JOURNAL ARTICLE
Adélaïde Lerebours, Samuel Robson, Colin Sharpe, Liubov Nagorskaya, Dmitri Gudkov, Charlotte Haynes-Lovatt, Jim T Smith
Fish have been highly exposed to radiation in freshwater systems after the Chernobyl Nuclear Power Plant (NPP) accident in 1986 and in freshwater and marine systems after the more recent Fukushima NPP accident in 2011. In the years after the accident, the radioactivity levels rapidly declined due to radioactive decay and environmental processes, but chronic lower dose exposures persisted. To gain insights into the long-term effects of environmental low dose radiation on fish ovaries development, a high-throughput transcriptomic approach including a de novo assembly was applied to different gonad phenotypes of female perch: developed gonads from reference lakes, developed/irradiated from medium contaminated lake, and both developed/irradiated and undeveloped from more highly contaminated lakes...
August 18, 2020: Environmental Science & Technology
https://read.qxmd.com/read/32245519/full-term-low-birth-weight-infants-have-differentially-hypermethylated-dna-related-to-immune-system-and-organ-growth-a-comparison-with-full-term-normal-birth-weight-infants
#31
COMPARATIVE STUDY
Ikuyo Hayashi, Ken Yamaguchi, Masahiro Sumitomo, Kenji Takakura, Narumi Nagai, Naoki Sakane
OBJECTIVE: Low birth weight (LBW) is a major public health issue as it increases the risk of noncommunicable diseases throughout life. However, the genome-wide DNA methylation patterns of full-term LBW infants (FT-LBWs) are still unclear. This exploratory study aimed to analyze the DNA methylation differences in FT-LBWs compared with those in full-term normal birth weight infants (FT-NBWs) whose mothers were nonsmokers and had no complications. Initially, 702 Japanese women with singleton pregnancies were recruited...
April 3, 2020: BMC Research Notes
https://read.qxmd.com/read/32095815/involvement-of-hus1-in-the-chain-elongation-step-of-dna-replication-after-exposure-to-camptothecin-or-ionizing-radiation
#32
JOURNAL ARTICLE
Xiang Wang, Jun Guan, Baocheng Hu, Robert S Weiss, George Iliakis, Ya Wang
No abstract text is available yet for this article.
February 25, 2020: Nucleic Acids Research
https://read.qxmd.com/read/31843500/amplification-of-7p12-is-associated-with-pathologic-nonresponse-to-neoadjuvant-chemotherapy-in-muscle-invasive-bladder-cancer
#33
JOURNAL ARTICLE
Renate Pichler, Andrea K Lindner, Eva Compérat, Peter Obrist, Georg Schäfer, Tilman Todenhöfer, Wolfgang Horninger, Zoran Culig, Gerold Untergasser
Pathologic downstaging (pDS) to neoadjuvant chemotherapy (NAC) is one of the most important predictors of survival in muscle-invasive bladder cancer (MIBC). The use of NAC is limited as pDS is only achieved in 30% to 40% of cases and predictive biomarkers are still lacking. We performed a comprehensive immunomolecular biomarker analysis to characterize the role of immune cells and inhibitory checkpoints, genome-wide frequencies of copy number alterations, mutational signatures in whole exome, and tumor mutational burden in predicting NAC response...
December 13, 2019: American Journal of Pathology
https://read.qxmd.com/read/31776186/structure-of-the-rad9-rad1-hus1-checkpoint-clamp-bound-to-rhino-sheds-light-on-the-other-side-of-the-dna-clamp
#34
JOURNAL ARTICLE
Kodai Hara, Nao Iida, Ryota Tamafune, Eiji Ohashi, Hitomi Sakurai, Yoshinobu Ishikawa, Asami Hishiki, Hiroshi Hashimoto
DNA clamp, a highly conserved ring-shaped protein, binds dsDNA within its central pore. Also, DNA clamp interacts with various nuclear proteins on its front, thereby stimulating their enzymatic activities and biological functions. It has been assumed that the DNA clamp is a functionally single-faced ring from bacteria to humans. Here, we report the crystal structure of the heterotrimeric RAD9-RAD1-HUS1 (9-1-1) checkpoint clamp bound to a peptide of RHINO, a recently identified cancer-related protein that interacts with 9-1-1 and promotes activation of the DNA damage checkpoint...
January 24, 2020: Journal of Biological Chemistry
https://read.qxmd.com/read/31624983/the-role-of-dna-repair-genes-in-radiation-induced-adaptive-response-in-drosophila-melanogaster-is-differential-and-conditional
#35
JOURNAL ARTICLE
Liubov Koval, Ekaterina Proshkina, Mikhail Shaposhnikov, Alexey Moskalev
Studies in human and mammalian cell cultures have shown that induction of DNA repair mechanisms is required for the formation of stimulation effects of low doses of ionizing radiation, named "hormesis". Nevertheless, the role of cellular defense mechanisms in the formation of radiation-induced hormesis at the level of whole organism remains poorly studied. The aim of this work was to investigate the role of genes involved in different mechanisms and stages of DNA repair in radioadaptive response and radiation hormesis by lifespan parameters in Drosophila melanogaster...
October 17, 2019: Biogerontology
https://read.qxmd.com/read/31574972/investigation-of-the-possible-role-of-rad9-in-post-diapaused-embryonic-development-of-the-brine-shrimp-artemia-sinica
#36
JOURNAL ARTICLE
Huifang Huang, Ce Chen, Feng Yao, Xiuling Li, Yanan Wang, Yuting Shao, Xinyao Wang, Xingzheng Zhang, Tao Jiang, Lin Hou
BACKGROUND: The cell cycle checkpoint protein RAD9 is a vital cell cycle regulator in eukaryotic cells. RAD9 is involved in diverse cellular functions by oligomer or monomer. However, the specific mechanism of its activity remains unknown in crustaceans, especially in embryonic diapause resumption of the brine shrimp Artemia sinica . METHODS AND RESULTS: In the present article, a 1238 bp full-length cDNA of As -RAD9 gene, encoding 376 amino acids, was obtained from A...
September 30, 2019: Genes
https://read.qxmd.com/read/31538680/the-fission-yeast-fhit-homolog-affects-checkpoint-control-of-proliferation-and-is-regulated-by-mitochondrial-electron-transport
#37
JOURNAL ARTICLE
Johanna Johansson Sjölander, Per Sunnerhagen
Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early stage cancers. The functions of the FHIT protein have however remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related to checkpoint control and oxidative metabolism. In sublethal concentrations of DNA damaging agents, aph1Δ mutants grew with a substantially shorter lag phase. In aph1Δ mutants carrying a hypomorphic allele of cds1 (fission yeast homolog of Chk2), in addition increased chromosome fragmentation and missegregation were found...
September 20, 2019: Cell Biology International
https://read.qxmd.com/read/31423183/knockdown-of-long-non-coding-rna-pvt1-inhibits-the-proliferation-of-raji-cells-through-cell-cycle-regulation
#38
JOURNAL ARTICLE
Chanli Zheng, Yu Xiao, Yangqiu Li, Dongmei He
Long non-coding RNA plasmacytoma variant translocation 1 ( PVT1 ) has been reported to be associated with oncogenesis. However, the functional role of PVT1 in Burkitt lymphoma has not yet been addressed. The purpose of the present study was to investigate the effect of PVT1 knockdown by small interfering RNA (siRNA) on the proliferation of Burkitt lymphoma Raji cells and to explore its possible mechanism of action. An effective siRNA targeting PVT1 was screened and the corresponding short hairpin RNA (shRNA) was reconstructed into a lentiviral vector...
August 2019: Oncology Letters
https://read.qxmd.com/read/31417910/isoproterenol-induced-cardiac-diastolic-dysfunction-in-mice-a-systems-genetics-analysis
#39
JOURNAL ARTICLE
Jessica Wang, Adriana Huertas-Vazquez, Yibin Wang, Aldons J Lusis
We examined an isoproterenol heart failure model across a panel of diverse inbred strains of mice, the Hybrid Mouse Diversity Panel (HMDP), using left atrial (LA) and lung weights as well as echocardiogram parameters as surrogates for cardiac diastolic function. We identified gene transcripts that significantly correlated with diastolic function. In addition, we mapped echocardiographic parameters associated with diastolic function. We identified a locus near Tns3-Hus1 to be associated with baseline E/A ratio in mice ( p = 1...
2019: Frontiers in Cardiovascular Medicine
https://read.qxmd.com/read/31182279/the-structure-of-the-checkpoint-clamp-9-1-1-complex-and-clamp-loader-rad24-rfc-in-saccharomyces-cerevisiae
#40
JOURNAL ARTICLE
Wei Liu
The 9-1-1 complex is a circular heterotrimeric complex composed of Rad9-Hus1-Rad1. In response to DNA damage, the 9-1-1 complex will be loaded onto the DNA damage site by clamp loader Rad24-RFC to activate the cell cycle checkpoint. The C-terminal of Ddc1/Rad9 is critical for checkpoint activation. However, there is little structural information about the intact 9-1-1 complex and the interaction with Rad24-RFC. Here, we determined the structure of the intact 9-1-1 complex in S. cerevisiae by cryo-Electron Microscopy (cryo-EM) and identified the Ddc1 C-tail module for the first time...
June 7, 2019: Biochemical and Biophysical Research Communications
keyword
keyword
109960
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.