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https://www.readbyqxmd.com/read/27904423/perceived-stress-among-malaysian-railway-workers
#1
Sami Abdo Radman Al-Dubai, Kurubaran Ganasegeran, Ramadan Elkalmi, Mohammed Alshakka, Nizar Ghanem
BACKGROUND: Stress is a common work-related problem worldwide. Railway workers are predominantly stressed due to their rigid protocols and limited rest opportunities. This study aimed to determine the level of, and factors associated with, stress among railway workers in Malaysia. METHODS: A cross-sectional study was conducted among all 729 railway workers employed at Keretapi Tanah Melayu Berhad (KTMB) in eight states in Malaysia via a postal survey. The self-administered questionnaire consisted of socio-demographic characteristics and the validated Malay version of Perceived Stress Scale-10 (PSS-10)...
September 2016: Malaysian Journal of Medical Sciences: MJMS
https://www.readbyqxmd.com/read/27898729/rna-contaminates-glycosaminoglycans-extracted-from-cells-and-tissues
#2
Jasper J van Gemst, Markus A Loeven, Mark J J de Graaf, Jo H M Berden, Ton J Rabelink, Cornelis H Smit, Johan van der Vlag
Glycosaminoglycans (GAGs) are linear negatively charged polysaccharides and important components of extracellular matrices and cell surface glycan layers such as the endothelial glycocalyx. The GAG family includes sulfated heparin, heparan sulfate (HS), dermatan sulfate (DS), chondroitin sulfate (CS), keratan sulfate, and non-sulfated hyaluronan. Because relative expression of GAGs is dependent on cell-type and niche, isolating GAGs from cell cultures and tissues may provide insight into cell- and tissue-specific GAG structure and functions...
2016: PloS One
https://www.readbyqxmd.com/read/27871390/eco-friendly-intracellular-biosynthesis-of-cds-quantum-dots-without-changing-escherichia-coli-s-antibiotic-resistance
#3
Zheng-Yu Yan, Qing-Qing Du, Jing Qian, Dong-Yu Wan, Sheng-Mei Wu
In the paper, a green and efficient biosynthetical technique was reported for preparing cadmium sulfide (CdS) quantum dots, in which Escherichia coli (E. coli) was chosen as a biomatrix. Fluorescence emission spectra and fluorescent microscopic photographs revealed that as-produced CdS quantum dots had an optimum fluorescence emission peak located at 470nm and emitted a blue-green fluorescence under ultraviolet excitation. After extracted from bacterial cells and located the nanocrystals' foci in vivo, the CdS quantum dots showed a uniform size distribution by transmission electron microscope...
January 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27846786/systematic-comparison-of-protocols-for-the-preparation-of-human-articular-cartilage-for-use-as-scaffold-material-in-cartilage-tissue-engineering
#4
Cornelia Schneider, Johannes Lehmann, Gerjo van Osch, Florian Hildner, Andreas Herbert Teuschl, Xavier Monforte, David Miosga, Patrick Heimel, Eleni Priglinger, Heinz Redl, Susanne Wolbank, Sylvia Nürnberger
Natural extracellular matrix (ECM) derived biomaterials from decellularized allogenic tissues are of increasing interest for tissue engineering since their structure and composition provide a complexity that is not achievable with current manufacturing techniques. The prerequisite to bring allogenic tissue from bench to bedside as a functional biomaterial is the full removal of cells while preserving most of its native characteristics such as structure and composition. The exceptionally dense structure of articular cartilage, however, poses a special challenge for decellularisation, scaffold preparation and reseeding...
November 15, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27836592/a-novel-signal-on-off-sensing-platform-for-selective-detection-of-thrombin-based-on-target-induced-ratiometric-electrochemical-biosensing-and-bio-bar-coded-nanoprobe-amplification-strategy
#5
Lanlan Wang, Rongna Ma, Liushan Jiang, Liping Jia, Wenli Jia, Huaisheng Wang
A novel dual-signal ratiometric electrochemical aptasensor for highly sensitive and selective detection of thrombin has been designed on the basis of signal-on and signal-off strategy. Ferrocene labeled hairpin probe (Fc-HP), thrombin aptamer and methyl blue labeled bio-bar-coded AuNPs (MB-P3-AuNPs) were rationally introduced for the construction of the assay platform, which combined the advantages of the recognition of aptamer, the amplification of bio-bar-coded nanoprobe, and the ratiometric signaling readout...
November 1, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27806478/effects-of-a-protocol-for-the-preparation-of-oxygen-moisturizing-chambers-on-the-count-of-its-bacteria-colony
#6
Reyhaneh Alikhani, Mohsen Shahriari, Sina Mobasherizadeh
This study aims to evaluate the effect of the protocol for the preparation of oxygen-moisturizing chamber on the count of its bacteria.This study is a double- group clinical trial before and after intervention, which was carried out in the neurology ward of an educational hospital in Esfahan from July to December 2015. In this study, 64 oxygen humidifying chambers were studied in terms of number and type of microorganisms in two groups of 32 during three phases, before, immediately after, and 6 hours after its intervention...
June 30, 2016: Global Journal of Health Science
https://www.readbyqxmd.com/read/27801518/detection-of-proteins-on-blot-membranes
#7
Aaron Goldman, Sandra Harper, David W Speicher
Staining of blot membranes enables the visualization of bound proteins. Proteins are usually transferred to blot membranes by electroblotting, by direct spotting of protein solutions, or by contact blots. Staining allows the efficiency of transfer to the membrane to be monitored. This unit describes protocols for staining proteins after electroblotting from polyacrylamide gels to blot membranes such as polyvinylidene difluoride (PVDF), nitrocellulose, or nylon membranes. The same methods can be used if proteins are directly spotted, either manually or using robotics...
November 1, 2016: Current Protocols in Protein Science
https://www.readbyqxmd.com/read/27785596/the-diagnostic-accuracy-of-chromatic-pupillary-light-responses-in-diseases-of-the-outer-and-inner-retina
#8
Paul Richter, Helmut Wilhelm, Tobias Peters, Holger Luedtke, Anne Kurtenbach, Herbert Jaegle, Barbara Wilhelm
PURPOSE: To compare the chromatic pupillary light responses (PLR) in healthy subjects with those from patients with diseases of the outer or inner retina under various stimulus conditions, and to ascertain the parameters required to optimally distinguish between disease and control groups. METHODS: Fifteen patients with retinitis pigmentosa (RP), 19 patients with optic nerve disease (ON), and 16 healthy subjects were enrolled in this prospective study. ON included optic neuritis (NNO) and non-arteritic anterior ischemic optic neuropathy (NAION)...
October 26, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27785458/aniline-blue-and-calcofluor-white-staining-of-callose-and-cellulose-in-the-streptophyte-green-algae-zygnema-and-klebsormidium
#9
Klaus Herburger, Andreas Holzinger
Plant including green algal cells are surrounded by a cell wall, which is a diverse composite of complex polysaccharides and crucial for their function and survival. Here we describe two simple protocols to visualize callose (β-1→3-glucan) and cellulose (β-1→4-glucan) and related polysaccharides in the cell walls of streptophyte green algae by using standard dyes and epifluorescence microscopy.
October 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/27774933/-acquirement-of-autologous-murine-cytotoxic-t-lymphocytes-via-cryopreservation-of-lymphocytes
#10
Lei Wang, Na Peng, Xiaoyan Hu, Wentao Liang, Kai Liang, Guizhu Peng
Objective To evaluate the effects of cryopreservation on the proliferation and killing activity of lymphocytes, and explore a novel protocol of preparing autologous mouse cytotoxic T lymphocytes (CTLs). Methods Mononuclear cells derived from spleen (5.0×10(6)/mL) were cryopreserved in CELLBANKER2 for 6 days and recovered in water bath at 39DegreesCelsius. The fresh lymphocytes and post-cryopreservation lymphocytes were induced by CD3 mAb (100 ng/mL) and recombinant mouse interleukin 2 (rmIL-2, 100 ng/mL) to obtain cytokine-induced killer cells (CIKs)...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27768053/an-alternative-approach-for-sample-preparation-with-low-cell-number-for-tem-analysis
#11
Sachin Kumar, Marie-Dominique Filippi
Transmission electron microscopy (TEM) provides details of the cellular organization and ultrastructure. However, TEM analysis of rare cell populations, especially cells in suspension such as hematopoietic stem cells (HSCs), remains limited due to the requirement of a high cell number during sample preparation. There are a few cytospin or monolayer approaches for TEM analysis from scarce samples, but these approaches fail to get significant quantitative data from the limited number of cells. Here, an alternative and novel approach for sample preparation in TEM studies is described for rare cell populations that enables quantitative analysis...
October 12, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27759836/advances-in-lung-ultrasound
#12
Miguel José Francisco, Antonio Rahal, Fabio Augusto Cardillo Vieira, Paulo Savoia Dias da Silva, Marcelo Buarque de Gusmão Funari
Ultrasound examination of the chest has advanced in recent decades. This imaging modality is currently used to diagnose several pathological conditions and provides qualitative and quantitative information. Acoustic barriers represented by the aerated lungs and the bony framework of the chest generate well-described sonographic artifacts that can be used as diagnostic aids. The normal pleural line and A, B, C, E and Z lines (also known as false B lines) are artifacts with specific characteristics. Lung consolidation and pneumothorax sonographic patterns are also well established...
July 2016: Einstein
https://www.readbyqxmd.com/read/27756051/preparation-of-99m-tc-isosulfan-blue-for-lymph-node-localization-in-rats-99m-tc-isosulfan-blue-for-lymph-node-localization
#13
Derya İlem-Özdemir, Ulkem Yararbas, Baha Zengel, Gökhan Ertan, Makbule Asikoglu
The sentinel lymph node (SLN) is defined as the first regional lymph node to receive lymphatic drainage from a malignant tumor. Therefore, this node is a "sentinel" for second metastatic lymph node stations and for labeling regional tumor spread. For SLN detection, many surgeons preferred a combination of a preoperative injection of radiolabeled colloid and the intraoperative injection of blue dye. Under this combination protocol, nodes are considered to be "sentinel nodes" if they are radioactive and blue...
October 11, 2016: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/27751961/light-induced-retinal-damage-using-different-light-sources-protocols-and-rat-strains-reveals-led-phototoxicity
#14
A Krigel, M Berdugo, E Picard, R Levy-Boukris, I Jaadane, L Jonet, M Dernigoghossian, C Andrieu-Soler, A Torriglia, F Behar-Cohen
To save energy, the European directives from the Eco-design of Energy Using Products (2005/32/CE) have recommended the replacement of incandescent lamps by more economic devices such as Light Emitting Diodes (LEDs). However, the emission spectrum of these devices is enriched in blue radiations, known to be potentially dangerous to the retina. Recent studies showed that light exposure contributes to the onset of early stages of age-related macular degeneration (AMD). Here, we investigate, in albinos and pigmented rats, the effects of different exposure protocols...
December 17, 2016: Neuroscience
https://www.readbyqxmd.com/read/27747683/difficulty-using-smart-pump-logs-to-recreate-a-patient-safety-event-case-study-and-considerations-for-pump-enhancements
#15
Andrew A M Ibey, Derek Andrews, Barb Ferreira
The authors present a case in which a physical anomaly with an infusion pump resulted in an unforeseen fault that the nurse's attempts to resolve unknowingly exacerbated. This case study presents the first report in the literature to detail the difficulty in recreating a patient safety event using smart pump logs, support server continuous quality improvement (CQI) data, and the drug order entry system to elucidate the clinical scenario. A 75-year-old male patient presented to a major teaching hospital and was admitted to the intensive care unit (ICU) with a massive gastrointestinal bleed and myocardial infarction, then stabilized...
December 2016: Drug Safety—Case Reports
https://www.readbyqxmd.com/read/27738121/listeria-monocytogenes-infection-of-hd11-chicken-macrophage-like-cells
#16
N A Jarvis, J R Donaldson, C A O'Bryan, S C Ricke, P G Crandall
Listeria monocytogenes can be carried by and infect poultry, although the clinical disease in birds is rare. Escape from macrophage phagocytosis is a key step in pathogenesis for L. monocytogenes Therefore, we investigated the infection of the chicken macrophage-like cell line HD11 with 2 strains of L. monocytogenes EGD-e and Scott A. After infection, L. monocytogenes was quantified by spread plating and HD11 was quantified with trypan blue exclusion stain before enumeration. The standard macrophage killing protocols require washing the cell monolayers 3 times with PBS, which was found to negatively influence HD11 monolayers...
October 12, 2016: Poultry Science
https://www.readbyqxmd.com/read/27737438/assessment-of-scaffolding-properties-for-chondrogenic-differentiation-of-adipose-derived-mesenchymal-stem-cells-in-nasal-reconstruction
#17
Serban San-Marina, Ayushman Sharma, Stephen G Voss, Jeffrey R Janus, Grant S Hamilton
Importance: Nasal reconstruction in patients who are missing a significant amount of structural nasal support remains a difficult challenge. One challenge is the deficiency of cartilage left within the nose as a consequence of rhinectomy or a midline destructive disease. Historically, the standard donor source for large quantities of native cartilage has been costal cartilage. Objective: To enable the development of protocols for new mesenchymal stem cell technologies as alternative procedures with reduced donor site morbidity, risk of infection and extrusion...
October 13, 2016: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/27737405/methylene-blue-to-treat-protamine-induced-anaphylaxis-reactions-an-experimental-study-in-pigs
#18
Agnes Afrodite S Albuquerque, Edson A Margarido, Antonio Carlos Menardi, Adilson Scorzoni, Andrea Carla Celotto, Alfredo J Rodrigues, Walter Vilella A Vicente, Paulo Roberto B Evora
Objective: To examine if methylene blue (MB) can counteract or prevent protamine (P) cardiovascular effects. Methods: The protocol included five heparinized pig groups: Group Sham -without any drug; Group MB - MB 3 mg/kg infusion; Group P - protamine; Group P/MB - MB after protamine; Group MB/P - MB before protamine. Nitric oxide levels were obtained by the nitric oxide/ozone chemiluminescence method, performed using the Nitric Oxide Analizer 280i (Sievers, Boulder, CO, USA)...
May 2016: Brazilian Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/27730682/high-nuclear-organometallic-copper-i-alkynide-clusters-thermochromic-near-infrared-luminescence-and-solution-stability
#19
Hong-Yan Zhuo, Hai-Feng Su, Zhao-Zhen Cao, Wei Liu, Shu-Ao Wang, Lei Feng, Gui-Lin Zhuang, Shui-Chao Lin, Mohamedally Kurmoo, Chen-Ho Tung, Di Sun, Lan-Sun Zheng
Cu(CF3 COO)2 reacts with tert-butylacetylene (tBuC≡CH) in methanol in the presence of metallic copper powder to give two air-stable clusters, [Cu(I)15 (tBuC≡C)10 (CF3 COO)5 ]⋅tBuC≡CH (1) and [Cu(I)16 (tBuC≡C)12 (CF3 COO)4 (CH3 OH)2 ] (2). The assembly process involves in situ comproportionation reaction between Cu(2+) and Cu(0) and the formation of two different clusters is controlled by reactants concentration. The clusters consist of Cu15 and Cu16 cores co-stabilized by strong by σ- and π-bonded tert-butylethynide and CF3 COO(-) (together with methanol molecule in 2)...
October 12, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27729939/is-blue-dye-still-required-during-sentinel-lymph-node-biopsy-for-breast-cancer
#20
Mirjam Cl Peek, Tibor Kovacs, Rose Baker, Hisham Hamed, Ash Kothari, Michael Douek
BACKGROUND: In early breast cancer, the optimal technique for sentinel lymph node biopsy (SLNB) is the combined technique (radioisotope and Patent Blue V) which achieves high identification rates. Despite this, many centres have decided to stop using blue dye due to blue-dye-related complications (tattoo, anaphylaxis). We evaluated the SLNB identification rate using the combined technique with and without Patent Blue V and the blue-dye-related complication rates. METHODS: Clinical and histological data were analysed on patients undergoing SLNB between March 2014 and April 2015...
2016: Ecancermedicalscience
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