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https://www.readbyqxmd.com/read/28540349/dataset-of-protein-species-from-human-liver
#1
Stanislav Naryzhny, Maria Maynskova, Victor Zgoda, Alexander Archakov
This article contains data related to the research article entitled "Zipf׳s law in proteomics" (Naryzhny et al., 2017) [1]. The protein composition in the human liver or hepatocarcinoma (HepG2) cells extracts was estimated using a filter-aided sample preparation (FASP) protocol. The protein species/proteoform composition in the human liver was determined by two-dimensional electrophoresis (2-DE) followed by Electrospray Ionization Liquid Chromatography-Tandem Mass Spectrometry (ESI LC-MS/MS). In the case of two-dimensional electrophoresis (2-DE), the gel was stained with Coomassie Brilliant Blue R350, and image analysis was performed with ImageMaster 2D Platinum software (GE Healthcare)...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28526089/adipose-derived-mesenchymal-stem-cells-from-liposuction-and-resected-fat-are-feasible-sources-for-regenerative-medicine
#2
Sandra Schneider, Marina Unger, Martijn van Griensven, Elizabeth R Balmayor
BACKGROUND: The use of mesenchymal stem cells (MSCs) in research and in regenerative medicine has progressed. Bone marrow as a source has drawbacks because of subsequent morbidities. An easily accessible and valuable source is adipose tissue. This type of tissue contains a high number of MSCs, and obtaining higher quantities of tissue is more feasible. Fat tissue can be harvested using different methods such as liposuction and resection. First, a detailed isolation protocol with complete characterization is described...
May 19, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28518109/assessment-of-neuronal-viability-using-fluorescein-diacetate-propidium-iodide-double-staining-in-cerebellar-granule-neuron-culture
#3
Lin Jiajia, Mak Shinghung, Zheng Jiacheng, Wang Jialing, Xu Dilin, Hu Shengquan, Zhang Zaijun, Wang Qinwen, Han Yifan, Cui Wei
Primary cultured Cerebellar Granule Neurons (CGNs) have been widely used as an in vitro model in neuroscience and neuropharmacology research. However, the co-existence of glial cells and neurons in CGN culture might lead to biases in the accurate assessment of neuronal viability. Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining has been used to measure cell viability by simultaneously evaluating the viable and dead cells. We used FDA-PI double staining to improve the sensitivities of the colorimetric assays and to evaluate neuronal viability in CGNs...
May 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518105/murine-lymphocyte-labeling-by-64cu-antibody-receptor-targeting-for-in-vivo-cell-trafficking-by-pet-ct
#4
Sabrina H L Hoffmann, Andreas Maurer, Dorothea I Reck, Gerald Reischl, Bernd J Pichler, Manfred Kneilling, Christoph M Griessinger
This protocol illustrates the production of (64)Cu and the chelator conjugation/radiolabeling of a monoclonal antibody (mAb) followed by murine lymphocyte cell culture and (64)Cu-antibody receptor targeting of the cells. In vitro evaluation of the radiolabel and non-invasive in vivo cell tracking in an animal model of an airway delayed-type hypersensitivity reaction (DTHR) by PET/CT are described. In detail, the conjugation of a mAb with the chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) is shown...
April 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518072/in-vivo-investigation-of-antimicrobial-blue-light-therapy-for-multidrug-resistant-acinetobacter-baumannii-burn-infections-using-bioluminescence-imaging
#5
Yucheng Wang, Olivia D Harrington, Ying Wang, Clinton K Murray, Michael R Hamblin, Tianhong Dai
Burn infections continue to be an important cause of morbidity and mortality. The increasing emergence of multidrug-resistant (MDR) bacteria has led to the frequent failure of traditional antibiotic treatments. Alternative therapeutics are urgently needed to tackle MDR bacteria. An innovative non-antibiotic approach, antimicrobial blue light (aBL), has shown promising effectiveness against MDR infections. The mechanism of action of aBL is not yet well understood. It is commonly hypothesized that naturally occurring endogenous photosensitizing chromophores in bacteria (e...
April 28, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516115/a-modified-chromogenic-assay-for-determination-of-the-ratio-of-free-intracellular-nad-nadh-in-streptococcus-mutans
#6
Jonathon L Baker, Roberta C Faustoferri, Robert G Quivey
Nicotinamide adenine dinucleotide is a coenzyme present in all kingdoms of life and exists in two forms: oxidized (NAD(+)) and reduced (NADH). NAD(H) is involved in a multitude of essential metabolic redox reactions, providing oxidizing or reducing equivalents. The ratio of free intracellular NAD(+)/NADH is fundamentally important in the maintenance of cellular redox homeostasis (Ying, 2008). Various chromogenic cycling assays have been used to determine the ratio of NAD(+)/NADH in both bacterial and mammalian cells for more than forty years (Bernofsky and Swan, 1973; Nisselbaum and Green, 1969)...
August 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28516113/pit-assay-to-measure-the-bone-resorptive-activity-of-bone-marrow-derived-osteoclasts
#7
Alexander Vesprey, Wentian Yang
Although it is possible to use a tartrate-resistant acid phosphatase (TRAP) stain to assist in identifying osteoclasts, a separate method is needed to determine the bone resorption activity of osteoclasts. Since osteoclasts leave "pits" after bone matrix resorption (Charles et al., 2014), it is possible to stain pits as a method of measuring osteoclast bone resorption activity. The pit assay protocol enables researchers to stain bony slices that were co-cultured with osteoclasts with toluidine blue in order to allow the visualization, capture, and analysis of osteoclast resorptive activity based on the number, size and depth of pits (Zhou et al...
June 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28508640/construction-of-polysubstituted-1-2-dihydrocyclobuta-b-naphthalenes-and-1-2-dihydrocyclobuta-b-anthracenes-with-photoluminescence
#8
Dianpeng Chen, Lianpeng Zhang, Jinzhong Yao, Hongwei Zhou
A facile protocol for the synthesis of polysubstituted 1,2-dihydrocyclobuta[b]naphthalenes and 1,2-dihydrocyclobuta[b]anthracenes was developed via a sequence of base-promoted 1,5-H shift, intramolecular [2+2] cycloaddition and aromatization. The synthesized 1,2-dihydrocyclobuta[b]anthracenes exhibited bright blue emissions in solution, and strong yellow emissions in solid, which made them possible candidates for optoelectronic conjugated materials.
May 16, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28508364/a-novel-2-de-based-proteomic-analysis-to-identify-multiple-substrates-for-specific-protease-in-neuronal-cells
#9
Chiho Kim, Young J Oh
Proteolysis is a process where proteins are broken down into smaller polypeptides or amino acids, comprising one of the important posttranslational modifications of proteins. Since this process is exquisitely achieved by specialized enzymes called proteases under physiological conditions, abnormal protease activity and dysregulation of their substrate proteins are closely associated with a progression of several neurodegenerative diseases including Alzheimer disease, Parkinson disease, stroke, and spinal cord injury...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28508242/preclinical-study-of-a-cost-effective-photodynamic-therapy-protocol-for-treating-oral-candidoses
#10
Nathalia Ramos da Silva, Daniela Garcia Ribeiro, João Paulo Mardegan Issa, Karla Bonfá, Michelli Sobreiro Menezes, Viviane de Cássia Oliveira, Raphael Freitas de Souza
Photodynamic therapy (PDT) is a promising treatment for oral candidoses. Its use as an alternative to antifungals prevents several adverse effects, including microbial resistance. However, most PDT protocols do not employ devices and consumables commonly available in dental practice, thus influencing treatment affordability. This study aimed to determine the efficacy of a PDT method based on light curing units' blue LEDs combined to a plaque-disclosing composition (5% erythrosine) against C. albicans in culture and in a murine model of oral candidosis...
May 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28506653/inhibition-of-denture-plaque-deposition-on-complete-dentures-by-2-methacryloyloxyethyl-phosphorylcholine-polymer-coating-a-clinical-study
#11
Kenji Ikeya, Fuminori Iwasa, Yuuki Inoue, Miya Fukunishi, Nana Takahashi, Kazuhiko Ishihara, Kazuyoshi Baba
STATEMENT OF PROBLEM: Denture plaque-associated infections are regarded as a source of serious dental and medical complications in the elderly population. Methods of managing this problem are needed. PURPOSE: The purpose of this clinical study was to evaluate the effects of treatment with a 2-methacryloyloxyethyl phosphorylcholine polymer, PMBPAz, on plaque deposition in complete dentures. MATERIAL AND METHODS: The study protocol was approved by the Ethics Committee of Showa University (#2013-013)...
May 12, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28499155/simple-screening-protocol-for-identification-of-potential-mycoremediation-tools-for-the-elimination-of-polycyclic-aromatic-hydrocarbons-and-phenols-from-hyperalkalophile-industrial-effluents
#12
Ramón Alberto Batista-García, Vaidyanathan Vinoth Kumar, Arielle Ariste, Omar Eduardo Tovar-Herrera, Olivier Savary, Heidy Peidro-Guzmán, Deborah González-Abradelo, Stephen A Jackson, Alan D W Dobson, María Del Rayo Sánchez-Carbente, Jorge Luis Folch-Mallol, Roland Leduc, Hubert Cabana
A number of fungal strains belonging to the ascomycota, basidiomycota and zygomycota genera were subjected to an in vitro screening regime to assess their ligninolytic activity potential, with a view to their potential use in mycoremediation-based strategies to remove phenolic compounds and polycyclic aromatic hydrocarbons (PAHs) from industrial wastewaters. All six basidiomycetes completely decolorized remazol brilliant blue R (RBBR), while also testing positive in both the guaiacol and gallic acid tests indicating good levels of lignolytic activity...
May 9, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28498113/a-greenhouse-gas-source-of-surprising-significance-anthropogenic-co2-emissions-from-use-of-methanol-in-sewage-treatment
#13
John L Willis, Ahmed Al-Omari, Robert Bastian, Bill Brower, Christine DeBarbadillo, Sudhir Murthy, Christopher Peot, Zhiguo Yuan
The impact of methanol (CH3OH) as a source of anthropogenic carbon dioxide (CO2) in denitrification at wastewater treatment plants (WWTPs) has never been quantified. CH3OH is the most commonly purchased carbon source for sewage denitrification. Until recently, greenhouse gas (GHG) reporting protocols consistently ignored the liberation of anthropogenic CO2 attributable to CH3OH. This oversight can likely be attributed to a simplifying notion that CO2 produced through activated-sludge-process respiration is biogenic because most raw-sewage carbon is un-sequestered prior to entering a WWTP...
May 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28487316/atheroma-susceptible-to-thrombosis-exhibit-impaired-endothelial-permeability-in-vivo-as-assessed-by-nanoparticle-based-fluorescence-molecular-imaging
#14
Ashley F Stein-Merlob, Tetsuya Hara, Jason R McCarthy, Adam Mauskapf, James A Hamilton, Vasilis Ntziachristos, Peter Libby, Farouc A Jaffer
BACKGROUND: The role of local alterations in endothelial functional integrity in atherosclerosis remains incompletely understood. This study used nanoparticle-enhanced optical molecular imaging to probe in vivo mechanisms involving impaired endothelial barrier function in experimental atherothrombosis. METHODS AND RESULTS: Atherosclerosis was induced in rabbits (n=31) using aortic balloon injury and high-cholesterol diet. Rabbits received ultrasmall superparamagnetic iron oxide nanoparticles (CLIO) derivatized with a near-infrared fluorophore (CyAm7) 24 hours before near-infrared fluorescence imaging...
May 2017: Circulation. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28475089/effect-of-high-or-low-concentration-bleaching-agents-containing-calcium-and-or-fluoride-on-enamel-microhardness
#15
Isabella Spinardi Furlan, Enrico Coser Bridi, Flávia Lucisano Botelho do Amaral, Fabiana Mantovani Gomes França, Cecilia Pedroso Turssi, Roberta Tarkany Basting
The aim of this in vitro study was to evaluate enamel microhardness following bleaching treatments using either high- or low-concentration hydrogen peroxide (HP) agents containing calcium and/or fluoride. Sixty enamel blocks were bleached with 1 of 6 different bleaching agents (n = 10). The high-concentration HP agents were Whiteness HP Maxx (35% HP), Whiteness HP Blue (35% HP, 2% calcium gluconate), Pola Office+ (37.5% HP, 5% potassium nitrate), and Opalescence Boost (38% HP, 1.1% fluoride ion, 3% potassium nitrate)...
May 2017: General Dentistry
https://www.readbyqxmd.com/read/28472561/effect-of-steroid-and-serum-starvation-on-a-human-breast-cancer-adenocarcinoma-cell-line
#16
Maryam Nakhjavani, David J Stewart, Farshad H Shirazi
Breast cancer is one of the most frequent cancers in women. Although there are many treatment protocols for this disease, there are still many patients who do not respond satisfactorily to the current treatment protocols. A better understanding of the nature of the tumor cells might provide a guide for better therapeutic procedures. In this study, the effect of metabolic stress was assessed on the human adenocarcinoma MCF-7 cell line. The cells were seeded in culture plates and after reaching log phase of their growth, cells were exposed to phenol red free-media supplemented with 10%, 0...
May 2017: Journal of Experimental Therapeutics & Oncology
https://www.readbyqxmd.com/read/28456679/assembly-of-polymer-micelles-through-the-sol-gel-transition-for-effective-cancer-therapy
#17
Nisar Ul Khaliq, Keun Sang Oh, Febrina Carolina Sandra, Yeonhee Joo, Juhyung Lee, Youngro Byun, In-San Kim, Ick Chan Kwon, Jae Hong Seo, Sang Yoon Kim, Soon Hong Yuk
Photo-induced apoptosis-targeted chemotherapy (PIATC) was designed and characterized to propose a new protocol for improved chemotherapy. Intratumoral injection was selected as the mode of administration of the anticancer drug, doxorubicin (DOX). To extend the retention time of DOX at the tumor parenchyma, in-situ gel formation was induced through the sol-gel transition of the Pluronic NPs containing a prodrug of DOX or a photosensitizer. The prodrug (DEVD-S-DOX) was designed to be inactive with a peptide moiety (Aspartic acid-Glutamic acid-Valine-Aspartic acid: DEVD) linked to DOX and to be cleaved into free DOX by caspase-3 expressed with apoptosis...
April 26, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28448011/turbidimetry-on-human-washed-platelets-the-effect-of-the-pannexin1-inhibitor-brilliant-blue-fcf-on-collagen-induced-aggregation
#18
Filippo Molica, Séverine Nolli, Pierre Fontana, Brenda Renata Kwak
Turbidimetry is a laboratory technique that is applied to measure the aggregation of platelets suspended in either plasma (platelet-rich plasma, PRP) or in buffer (washed platelets), by the use of one or a combination of agonists. The use of washed platelets separated from their plasma environment and in the absence of anticoagulants allows for studying intrinsic platelet properties. Among the large panel of agonists, arachidonic acid (AA), adenosine di-phosphate (ADP), thrombin and collagen are the most frequently used...
April 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28433858/reduced-graphene-oxide-chitosan-aptamer-interface-as-new-platform-for-ultrasensitive-detection-of-human-epidermal-growth-factor-receptor-2
#19
Arezoo Tabasi, Abdollah Noorbakhsh, Ensiyeh Sharifi
The present work describes an ultrasensitive electrochemical aptamer-based assay for detection of human epidermal growth factor receptor 2 protein (HER2) cancer biomarker as a model analyte. Results show that the reduced graphene oxide-chitosan (rGO-Chit) film as a suitable electrode material possesses great favorable properties including high homogeneity, good stability, large surface area and high fraction of amine groups as aptamer binding sites. Various steps of aptasensor fabrication were characterized using microscopic, energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28427409/efficacies-of-dha-ppq-and-as-sp-in-patients-with-uncomplicated-plasmodium-falciparum-malaria-in-an-area-of-an-unstable-seasonal-transmission-in-sudan
#20
Abdelrahim O Mohamed, Muzamil M Abdel Hamid, Omer S Mohamed, Nuha S Elkando, Abdelmaroof Suliman, Mariam A Adam, Fahad Awad Ali Elnour, Elfatih M Malik
BACKGROUND: Artemisinin-based combination therapy (ACT), together with other control measures, have reduced the burden of falciparum malaria in sub-Saharan countries, including Sudan. Sudan adopted ACT in 2004 with a remarkable reduction in mortality due to falciparum malaria. However, emergence of resistance to the first-line treatment artesunate and sulfadoxine/pyrimethamine (AS/SP) has created new challenges to the control of malaria in Sudan. A search for an alternative drug of choice for treating uncomplicated malaria has become inevitable...
April 20, 2017: Malaria Journal
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