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https://www.readbyqxmd.com/read/29032785/two-glutathione-transferase-isoforms-isolated-from-juvenile-cysts-of-taenia-crassiceps-identification-purification-and-characterization
#1
G Maldonado, G Nava, A Plancarte
We identified and characterized the first two glutathione transferases (GSTs) isolated from juvenile cysts of Taenia crassiceps (EC 2.5.1.18). The two glutathione transferases (TcGST1 and TcGST2) were purified in a single-step protocol using glutathione (GSH)-sepharose chromatography in combination with a GSH gradient. The specific activities of TcGST1 and TcGST2 were 26 U mg-1 and 19 U mg-1, respectively, both at 25°C and pH 6.5 with 1-chloro-2,4-dinitrobenzene (CDNB) and GSH as substrates. The K m(CDNB) and K cat(CDNB) values for TcGST1 and TcGST2 (0...
October 16, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/29017759/differential-cytotoxic-effects-on-odontoblastic-cells-induced-by-self-adhesive-resin-cements-as-a-function-of-the-activation-protocol
#2
Paulo Henrique Perlatti D'Alpino, Gioconda Emanuella Diniz de Dantas Moura, Silvana Coelho de Arruda Barbosa, Lygia de Azevedo Marques, Marcos Nogueira Eberlin, Fábio Dupart Nascimento, Ivarne Luis Dos Santos Tersariol
OBJECTIVES: To evaluate the cytotoxic effects of exposing odontoblast cells to a variety of commercial self-adhesive cements polymerized using different activation modes. METHODS: Five cements: MaxCem Elite (MAX), Bifix SE (BSE), G-Cem LinkAce (GCE), Clearfil SA Luting (CAS), and RelyX U200 (U200) were mixed, dispensed into molds, and distributed in groups, according to polymerization protocols: immediate photoactivation; delayed photoactivation (10min self-curing plus light-activation); and chemical activation (no light exposure)...
October 7, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28991492/self-templated-synthesis-of-porous-ni-oh-2-nanocube-and-its-high-electrochemical-performance-for-supercapacitor
#3
Liang Li, Li Tan, Gengnan Li, Yumeng Zhang, Lili Liu
Porous Ni(OH)2 nanocubes were successfully fabricated by a simple self-sacrificial-template protocol using Ni-Co Prussian blue analogue (PBA) as precursor. When treated with NaOH, the simultaneous corrosion of Ni-Co PBA precursor and formation of amorphous Ni(OH)2 resulted in porous Ni(OH)2 nanocubes with uniform size of about 100 nm. Due to the large specific surface area and unique regular porous structure, the as-prepared materials showed large specific capacitance, relatively stable rate capability and long cycle stability when used as electrode materials for supercapacitors...
October 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28981792/dosimetry-with-salt-in-mixed-radiation-fields-of-photons-and-neutrons
#4
Daniela Ekendahl, Peter Rubovic, Pavel Žlebcík, Ondrej Huml, Helena Malá
Salt (NaCl) represents a radiation sensitive material with a considerable potential for dosimetry in mixed radiation fields of photons and neutrons. In consequence of a gamma radiation exposure, it exhibits a strong luminescence signal following stimulation with blue light. Optically stimulated luminescence (OSL) technique can be used for measurement. Photon dose reconstruction can be done using so-called Single-Aliquot Regenerative-dose protocol. However, a part of OSL signal is caused by NaCl self-irradiation resulting from neutron reactions...
August 22, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/28979900/comparison-of-the-effects-of-interleukin-1-on-equine-articular-cartilage-explants-and-cocultures-of-osteochondral-and-synovial-explants
#5
Christopher R Byron, Richard A Trahan
Osteoarthritis (OA) is a ubiquitous disease affecting many horses. The disease causes chronic pain and decreased performance for patients and great cost to owners for diagnosis and treatment. The most common treatments include systemic non-steroidal anti-inflammatory drugs and intra-articular injection of corticosteroids. There is excellent support for the palliative pain relief these treatments provide; however, they do not arrest progression and may in some instances hasten advancement of disease. Orthobiologic treatments have been investigated as potential OA treatments that may not only ameliorate pain but also prevent or reverse pathologic articular tissue changes...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28968293/standardizing-descemet-membrane-endothelial-keratoplasty-graft-preparation-method-in-the-eye-bank-experience-of-527-descemet-membrane-endothelial-keratoplasty-tissues
#6
Mohit Parekh, Mattia Baruzzo, Elisa Favaro, Davide Borroni, Stefano Ferrari, Diego Ponzin, Alessandro Ruzza
PURPOSE: To share the experience and provide a standardized protocol for Descemet membrane endothelial keratoplasty (DMEK) graft preparation. METHODS: A retrospective study based on 527 prestripped DMEK tissues that were prepared between 2014 and 2017. The experience of using different instruments and techniques has been described, and a standardized technique for preparing DMEK grafts has been identified. The tissues in general were prepared by superficially tapping the endothelial side with a Moria trephine (9...
September 29, 2017: Cornea
https://www.readbyqxmd.com/read/28956430/multiplexed-electrochemical-detection-of-mirnas-from-sera-of-glioma-patients-at-different-stages-via-the-novel-conjugates-of-conducting-magnetic-microbeads-and-diblock-oligonucleotide-modified-gold-nanoparticles
#7
Jingrui Wang, Zhixuan Lu, Hailin Tang, Ling Wu, Zixiao Wang, Minghua Wu, Xinyao Yi, Jianxiu Wang
MicroRNAs (miRNAs) serve as diagnostic and prognostic biomarkers for a wide variety of cancers. Via the novel conjugates of gold nanoparticle-coated magnetic microbeads (AuNP-MMBs) and the diblock oligonucleotide (ODN)-modified AuNPs, multiplexed electrochemical assay of miRNAs was performed. The hybridization to target miRNAs leads to the conformational change of the hairpin-structured ODN probes, and the attachment of the diblock ODN-modified AuNPs was achieved. By examining the oxidation peak currents of methylene blue (MB) and ferrocene (Fc) moieties residing on the diblock ODNs, simultaneous quantification of miRNA-182 and miRNA-381 was conducted...
October 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28927212/fluorescein-guided-neuroendoscopy-for-intraventricular-lesions-a-case-series
#8
Alessandro Fiorindi, Alessandro Boaro, Giulia Del Moro, Pierluigi Longatti
BACKGROUND: The benefits of neuroendoscopy in the pathological diagnosis of intra- and paraventricular tumors have already been shown in many neurosurgical studies. However, most authors agree that neuroendoscopic biopsies are not infrequently inconclusive due to small or inadequate samples, prompting the need for new diagnostic strategies. OBJECTIVE: To describe a technique not previously reported in the literature, combining neuroendoscopy with angiofluorescein guidance for the pathological diagnosis of intra- and paraventricular tumors...
April 1, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/28920612/visible-light-mediated-oxidative-demethylation-of-n-6-methyl-adenines
#9
Li-Jun Xie, Rui-Li Wang, Dong Wang, Li Liu, Liang Cheng
We report a simple protocol that affords oxidative demethylation of N(6)-methyl groups in N(6)-methyl adenines (m(6)A). The biologically compatible photocatalyst riboflavin prompts a highly selective C-H abstraction from N(6)-methyl in adenines under the irradiation of a visible blue LED light, affording a novel and highly selective biomimetic demethylation of m(6)A and related N-methyl adenine analogues.
September 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28899394/a-two-state-comparative-implementation-of-peer-support-intervention-to-link-veterans-to-health-related-services-after-incarceration-a-study-protocol
#10
Molly M Simmons, Benjamin G Fincke, Mari-Lynn Drainoni, Bo Kim, Tom Byrne, David Smelson, Kevin Casey, Marsha L Ellison, Christy Visher, Jessica Blue-Howells, D Keith McInnes
BACKGROUND: Approximately 600,000 persons are released from prison annually in the United States. Relatively few receive sufficient re-entry services and are at risk for unemployment, homelessness, poverty, substance abuse relapse and recidivism. Persons leaving prison who have a mental illness and/or a substance use disorder are particularly challenged. This project aims to create a peer mentor program to extend the reach and effectiveness of reentry services provided by the Department of Veterans' Affairs (VA)...
September 12, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28895060/cerebral-tissue-oxidative-ischemia-reperfusion-injury-in-connection-with-experimental-cardiac-arrest-and-cardiopulmonary-resuscitation-effect-of-mild-hypothermia-and-methylene-blue
#11
Lars Wiklund, Ranjana Patnaik, Aruna Sharma, Adriana Miclescu, Hari S Sharma
The present investigation is an expansion of previous studies which all share a basic experimental protocol of a porcine-induced cardiac arrest (CA) of 12 min followed by 8 min of cardiopulmonary resuscitation (CPR), different experimental treatments (immediate as well as postponed induced mild hypothermia and administration of much or less cool intravenous fluids), and a follow-up period of 3 h after which the animals were sacrificed. Another group of animals was studied according to the same protocol after 12-min CA and "standard CPR...
September 11, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28891701/sedative-effects-of-intranasal-midazolam-administration-in-wild-caught-blue-fronted-amazon-amazona-aestiva-and-orange-winged-amazon-amazona-amazonica-parrots
#12
Débora P H Schaffer, Nayone L L C de Araújo, Ana Cláudia S Raposo, Emanoel F Martins Filho, João Victor R Vieira, Arianne P Oriá
Safe and effective sedation protocols are important for chemical restraint of birds in clinical and diagnostic procedures, such as clinical evaluations, radiographic positioning, and blood collection. These protocols may reduce stress and ease the management of wild-caught birds, which are susceptible to injury or death when exposed to stressful situations. We compare the sedative effect of intranasal midazolam in wild-caught blue-fronted (Amazona aestiva) and orange-winged (Amazona amazonica) Amazon parrots...
September 2017: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28889295/analysis-of-c-di-gmp-levels-synthesized-by-a-photoreceptor-protein-in-response-to-different-light-qualities-using-an-in-vitro-enzymatic-assay
#13
Veronika Angerer, Lars-Oliver Essen, Annegret Wilde
Diguanylate cyclases are enzymes that use two GTP molecules to produce one molecule cyclic dimeric guanosine monophosphate (c-di-GMP). This cyclic dinucleotide is an ubiquitous prokaryotic second messenger that controls a variety of cell functions. Several proteins have been described which contain a photoreceptor domain fused to a diguanylate cyclase. The cyanobacterial light sensor Cph2 is responsible for the blue-light induced synthesis of c-di-GMP in Synechocystis sp. PCC 6803. Here, we provide a detailed protocol for an in vitro enzymatic assay with a purified photoreceptor protein using light as the crucial reaction parameter for c-di-GMP synthesis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28883989/osteoinductive-effects-of-preoperative-dexamethasone-in-human-dental-pulp-stem-cells-primary-culture
#14
Rani da Cunha Moretti, Monica Talarico Duailibi, Paulo Oliveira Martins, Jennifer Adriane Dos Santos, Silvio Eduardo Duailibi
AIM: The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques. Our objective was to evaluate the osteoinductive effect of DEX administered as a preoperative medication in primary cell culture of human dental pulp stem cell...
August 2017: Future Science OA
https://www.readbyqxmd.com/read/28875761/an-ultralow-dose-1-day-protocol-with-activities-lower-than-20-mbq-for-the-detection-of-sentinel-lymph-nodes-in-breast-cancer-experiences-after-150-cases
#15
Hans-Christian Kolberg, Shabnam Afsah, Thorsten Kuehn, Ute Winzer, Leyla Akpolat-Basci, Miltiades Stephanou, Sarah Wetzig, Oliver Hoffmann, Cornelia Liedtke
INTRODUCTION: Common protocols for the detection of sentinel lymph nodes in early breast cancer often include the injection of the tracer 1 day before surgery. In order to detect enough activity on the day of surgery, the applied activity in many protocols is as high as several hundred MBq. So far, very few protocols with an activity below 20 MBq have been reported. We developed an ultralow-dose 1-day protocol with a mean activity lower than 20 MBq in order to reduce radiation exposure for patients and staff...
January 1, 2017: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/28872692/coomassie-staining-provides-routine-sub-femtomole-in-gel-detection-of-intact-proteoforms-expanding-opportunities-for-genuine-top-down-proteomics
#16
Nour Noaman, Prabhodh S Abbineni, Michael Withers, Jens R Coorssen
Modified colloidal Coomassie Brilliant Blue (cCBB) staining utilising a novel destain protocol and near-infrared fluorescence detection (nIRFD) rivals the in-gel protein detection sensitivity (DS) of SYPRO Ruby. However, established DS estimates are likely inaccurate in terms of 2DE-resolved proteoform 'spots' since DS is routinely measured from comparatively diffuse protein 'bands' following wide-well 1DE. Here, cCBB DS for 2DE-based proteomics was more accurately determined using narrow-well 1DE. As precise estimates of protein standard monomer concentrations are essential for accurate quantitation, coupling UV absorbance with gel-based purity assessments is described...
September 5, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28867637/sinomenine-induced-histamine-release-like-anaphylactoid-reactions-are-blocked-by-tranilast-via-inhibiting-nf-%C3%AE%C2%BAb-signaling
#17
Lufen Huang, Yan Dong, Jianlin Wu, Peixun Wang, Hua Zhou, Ting Li, Liang Liu
Zhengqing Fengtongning (ZQFTN), the pharmaceutical preparation of sinomenine (SIN) derived from the medicinal plant Sinmenium acutum, is well-known in China as an effective treatment for rheumatoid arthritis (RA). However, its histamine-release anaphylactoid reactions (HRARs) occur often in some patients. Therefore, it is desirable to establish effective clinical protocols to manage such HRARs. In the study, rat models with systemic HRARs and local HRARs of the skin were established. The level of vascular permeability and mast cell numbers was determined by quantitative analysis using Evans blue dye and histological assays...
September 1, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28861856/role-of-sentinel-node-in-differentiated-thyroid-cancer-a-prospective-study-comparing-patent-blue-injection-technique-lymphoscintigraphy-and-the-combined-technique
#18
R Gelmini, M Campanelli, F Cabry, A Franceschetto, G Ceresini, L Ruffini, A Zaccaroni, P Del Rio
PURPOSE: The purpose of the present study was to evaluate the feasibility and reproducibility of the sentinel lymph node (SLNs) biopsy in differentiated thyroid cancer using patent blue injection, lymphoscintigraphy and the combined techniques. METHODS: Between January 2011 and January 2013, 82 consecutive patients were enrolled in our prospective multicentre study. Inclusion criteria were 18 years of age, preoperative diagnosis of differentiated thyroid carcinoma, no evidence of lymph node enlargement and multifocal neoplasm...
August 31, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28858288/simultaneous-lipid-and-content-mixing-assays-for-in-vitro-reconstitution-studies-of-synaptic-vesicle-fusion
#19
Xiaoxia Liu, Alpay Burak Seven, Junjie Xu, Victoria Esser, Lijing Su, Cong Ma, Josep Rizo
This protocol describes reconstitution assays to study how the neurotransmitter release machinery triggers Ca(2+)-dependent synaptic vesicle fusion. The assays monitor fusion between proteoliposomes containing the synaptic vesicle SNARE synaptobrevin (with or without the Ca(2+) sensor synaptotagmin-1) and proteoliposomes initially containing the plasma membrane SNAREs syntaxin-1 and soluble NSF attachment protein (SNAP)-25. Lipid mixing (from fluorescence de-quenching of Marina-Blue-labeled lipids) and content mixing (from development of fluorescence resonance energy transfer (FRET) between phycoerythrin-biotin (PhycoE-Biotin) and Cy5-streptavidin trapped in the two proteoliposome populations) are measured simultaneously to ensure that true, nonleaky membrane fusion is monitored...
September 2017: Nature Protocols
https://www.readbyqxmd.com/read/28837527/prestained-and-preloaded-dmek-grafts-an-evaluation-of-tissue-quality-and-stain-retention
#20
Dorian A Zeidenweber, Khoa D Tran, Christopher S Sales, Stephen W Wehrer, Michael D Straiko, Mark A Terry
PURPOSE: To examine endothelial cell damage and stain retention of prestained preloaded Descemet membrane endothelial keratoplasty (DMEK) grafts. METHODS: DMEK grafts were stained with trypan blue or left unstained before loading into a Straiko modified Jones tube. A protocol to stain preloaded grafts inside the modified Jones tube and resulting cell loss was also examined. Endothelial cell loss was determined by vital dye staining after 3 days of cold storage and compared between the 3 groups...
August 23, 2017: Cornea
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