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https://www.readbyqxmd.com/read/28220426/measuring-callose-deposition-an-indicator-of-cell-wall-reinforcement-during-bacterial-infection-in-arabidopsis
#1
Lin Jin, David M Mackey
The plant cell wall responds dynamically during interaction with various pathogens. Upon recognition of "nonself" components, plant cells deploy a variety of immune responses including cell wall fortification. Callose, a β-(1, 3)-D-glucan polymer, is a component of the material deposited at the site of infection between the plasma membrane and the preexisting cell wall that is hypothesized to serve as a physical barrier and platform for directed antimicrobial compound deposition. The defense-associated function of callose deposition is supported by its induction during pathogen-associated molecular patterns (PAMP)-triggered immunity (PTI) and its inhibition by defense suppressing virulence effectors...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28150881/non-gradient-blue-native-polyacrylamide-gel-electrophoresis
#2
Xiaoting Luo, Jinzi Wu, Zhen Jin, Liang-Jun Yan
Gradient blue native polyacrylamide gel electrophoresis (BN-PAGE) is a well established and widely used technique for activity analysis of high-molecular-weight proteins, protein complexes, and protein-protein interactions. Since its inception in the early 1990s, a variety of minor modifications have been made to this gradient gel analytical method. Here we provide a major modification of the method, which we call non-gradient BN-PAGE. The procedure, similar to that of non-gradient SDS-PAGE, is simple because there is no expensive gradient maker involved...
February 2, 2017: Current Protocols in Protein Science
https://www.readbyqxmd.com/read/28145483/determination-of-blue-light-induced-infrared-absorption-based-on-mode-matching-efficiency-in-an-optical-parametric-oscillator
#3
Yajun Wang, Wenhai Yang, Zhixiu Li, Yaohui Zheng
Non-classical squeezed states of light at a compatible atomic wavelength have a potential application in quantum information protocols for quantum states delaying or storaging. An optical parametric oscillator (OPO) with periodically poled potassium titanyl phosphate (PPKTP) is the most effective method for generating this squeezed state. However, it is a challege for the nonlinear interaction in PPKTP crystal at the D1 line of rubidium atomic, due to a strong blue-light-induced infrared absorption (BLIIRA)...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28139746/a-sensitive-single-enzyme-assay-system-using-the-non-ribosomal-peptide-synthetase-bpsa-for-measurement-of-l-glutamine-in-biological-samples
#4
Alistair S Brown, Katherine J Robins, David F Ackerley
The ability to rapidly, economically and accurately measure L-glutamine concentrations in biological samples is important for many areas of research, medicine or industry, however there is room for improvement on existing methods. We describe here how the enzyme BpsA, a single-module non-ribosomal peptide synthetase able to convert L-glutamine into the blue pigment indigoidine, can be used to accurately measure L-glutamine in biological samples. Although indigoidine has low solubility in aqueous solutions, meaning direct measurements of indigoidine synthesis do not reliably yield linear standard curves, we demonstrate that resolubilisation of the reaction end-products in DMSO overcomes this issue and that spontaneous reduction to colourless leuco-indigoidine occurs too slowly to interfere with assay accuracy...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28139513/transfusion-of-plasma-collected-at-late-phase-after-preconditioning-reduces-myocardial-infarct-size-induced-by-ischemia-reperfusion-in-rats-in-vivo
#5
Yang Zhao, Zhi-Nan Zheng, Chi-Wai Cheung, Zhi-Yi Zuo, San-Qing Jin
BACKGROUND: Plasma transfusion is a common clinical practice. Remote ischemic preconditioning (RIPC) protects organs against ischemia-reperfusion (IR) injury. Whether preconditioned plasma (PP), collected at late phase after RIPC, could protect organs against IR injury in vivo is unknown. This study explored whether transfusion of PP could reduce myocardial infarct size (IS) after IR in rat in vivo. METHODS: Eighty Lewis rats were randomized to eight groups (n = 10 for each group)...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28128400/mir-187-induces-apoptosis-of-siha-cervical-carcinoma-cells-by-downregulating-bcl-2
#6
C Y He, J Yang
Cervical carcinoma is a life-threatening illness posing considerable danger to women's health. microRNAs (miRNAs) have been shown to regulate multiple cellular events, including growth and proliferation, and miR-187 is thought to regulate the growth and apoptosis of certain cell types. Our study focused on the influence of miR-187 on the growth, proliferation, and apoptosis of SiHa cervical carcinoma cells, and explored the mechanism behind its pro-apoptotic effect. miR-187 and control (scrambled) miRNA were synthesized with a standard protocol and lipofected into SiHa cells...
January 23, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28128307/emergent-properties-of-giant-vesicles-formed-by-a-polymerization-induced-self-assembly-pisa-reaction
#7
Anders N Albertsen, Jan K Szymański, Juan Pérez-Mercader
Giant micrometer sized vesicles are of obvious interest to the natural sciences as well as engineering, having potential application in fields ranging from drug delivery to synthetic biology. Their formation often requires elaborate experimental techniques and attempts to obtain giant vesicles from chemical media in a one-pot fashion have so far led to much smaller nanoscale structures. Here we show that a tailored medium undergoing controlled radical polymerization is capable of forming giant polymer vesicles...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28125078/frequent-inactivating-germline-mutations-in-dna-repair-genes-in-patients-with-ewing-sarcoma
#8
Andrew S Brohl, Rajesh Patidar, Clesson E Turner, Xinyu Wen, Young K Song, Jun S Wei, Kathleen A Calzone, Javed Khan
PURPOSE: Ewing sarcoma is a small round blue cell tumor that is highly malignant and predominantly affects the adolescent and young adult population. It has long been suspected that a genetic predisposition exists for this cancer, but the germ-line genetic underpinnings of this disease have not been well established. METHODS: We performed germline variant analysis of whole-genome or whole-exome sequencing of samples from 175 patients affected by Ewing sarcoma. RESULTS: We discovered pathogenic or likely pathogenic germline mutations in 13...
January 26, 2017: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/28125005/isolation-and-characterization-of-a-phaseolus-vulgaris-trypsin-inhibitor-with-antiproliferative-activity-on-leukemia-and-lymphoma-cells
#9
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. "gold bean" with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dose-dependently inhibited trypsin with an IC50 value of 0.4 μM, and this activity was reduced in the presence of dithiothreitol in a dose- and time-dependent manner, signifying the importance of the disulfide linkage to the activity...
January 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28108569/five-year-progression-of-unilateral-age-related-macular-degeneration-to-bilateral-involvement-the-three-continent-amd-consortium-report
#10
Nichole Joachim, Johanna Maria Colijn, Annette Kifley, Kristine E Lee, Gabriëlle H S Buitendijk, Barbara E K Klein, Chelsea E Myers, Stacy M Meuer, Ava G Tan, Elizabeth G Holliday, John Attia, Gerald Liew, Sudha K Iyengar, Paulus T V M de Jong, Albert Hofman, Johannes R Vingerling, Paul Mitchell, Caroline C W Klaver, Ronald Klein, Jie Jin Wang
PURPOSE: To assess the 5-year progression from unilateral to bilateral age-related macular degeneration (AMD) and associated risk factors. DESIGN: Pooled data analyses of three prospective population-based cohorts, the Blue Mountains Eye Study, Beaver Dam Eye Study and Rotterdam Study. METHODS: Retinal photography and interview with comprehensive questionnaires were conducted at each visit of three studies. AMD was assessed following the modified Wisconsin AMD grading protocol...
January 20, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28101348/evans-blue-mediated-white-light-detection-of-non-muscle-invasive-bladder-cancer-a-preclinical-feasibility-and-safety-study-using-a-rat-bladder-urothelial-cell-carcinoma-model
#11
Sanne Elsen, Evelyne Lerut, Frank Van Der Aa, Ben Van Cleynenbreugel, Hendrik Van Poppel, Peter De Witte
Photodynamic diagnosis (PDD) improves the detection of non-muscle-invasive bladder cancer (NMIBC). However, white-light (WL) cystoscopy remains the technique routinely used in urological clinics. A more cost-effective but equally performant alternative to PDD may encompass the use of an intense tumoritropic dye in combination with WL cystoscopy. Using a preclinical setting, we investigated the practical aspects of the use of Evans blue (EB) dye for the possible future detection of NMIBC using WL cystoscopy...
December 2016: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/28099873/antimicrobial-photodynamic-therapy-as-an-adjunct-for-treatment-of-deep-carious-lesions-a-systematic-review
#12
REVIEW
Fabian Cieplik, Wolfgang Buchalla, Elmar Hellwig, Ali Al-Ahmad, Karl-Anton Hiller, Tim Maisch, Lamprini Karygianni
For deep carious lesions, a more conservative treatment modality ("selective caries removal") has been proposed, where only the heavily contaminated dentine is removed. In this regard, effective adjuncts for cavity disinfection such as the antimicrobial photodynamic therapy (aPDT) can be valuable clinically prior to definitive restoration. Therefore, the aim of this study was to systematically assess clinical studies on the effectiveness of aPDT as a supplementary tool in the treatment of deep caries lesions...
January 15, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28095557/the-photodynamic-therapy-experience-of-a-high-volume-laser-and-dermatologic-surgery-center
#13
David E Orbuch, Lauren Penn, Bradley S Bloom, Jeremy A Brauer, Daniel B Shin, Joshua Greenbaum, Leonard J Bernstein, Elliot T Weiss, Robert T Anolik, Roy G Geronemus
BACKGROUND: Photodynamic therapy (PDT) is an FDA approved treatment for actinic keratoses (AK's) although multiple off-label indi- cations are reported. Despite frequent use for AK's, no clear consensus exists regarding protocols for overall treatment parameters. METHODS: Retrospective chart review of 1,491 subjects who underwent PDT between 2007 and 2011 at a high volume laser surgery center. Demographic information, clinical history, treatment data, and subsequent diagnosis of skin cancers were recorded...
November 1, 2016: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28095551/acne-treatment-with-3-step-broadband-light-protocol
#14
Patrick Bitter
BACKGROUND AND OBJECTIVE: A new 3-step protocol using broadband light allows patients with moderate to severe in ammatory acne to avoid potential side effects of systemic acne treatments and the risks and uncertainties associated with laser and light treatments to date. The protocol also addresses acne scarring and, with appropriate modi cations, all skin types. METHODS: The protocol consists of 6 to 8 treatments performed with a single device that allows the user to select wavelength, spot size, uence, and pulse duration...
November 1, 2016: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28072432/closed-vitrification-system-as-a-platform-for-cryopreservation-of-tissue-engineered-constructs
#15
N A Trufanova, V S Zaikov, A V Zinchenko, A Yu Petrenko, Y A Petrenko
BACKGROUND: Cryopreservation of mesenchymal stromal cells (MSCs) and MSCs-based tissue engineered constructs (TECs) is a promising strategy for regenerative medicine. OBJECTIVE: To examine vitrification system consisting of multicomponent vitreous solution, closed type container, human adult MSCs and two-step exposure procedure as a platform for cryopreservation of MSCs-based TECs. MATERIALS AND METHODS: Vitrification properties of solutions were studied by visual analysis and calorimetry...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28065860/influence-of-ultrasonic-activation-over-final-irrigants-in-the-removal-of-photosensitizer-from-root-canal-walls-after-photodynamic-therapy
#16
Matheus Albino Souza, Bianca Pazinatto, Karolina Frick Bischoff, Huriel Scartazzini Palhano, Doglas Cecchin, José Antônio Poli de Figueiredo
INTRODUCTION: The aim of the present study was to evaluate the influence of ultrasonic activation (US) of the final irrigant in the removal of the photosensitizer from root canal walls after photodynamic therapy. METHODS: The root canals of 60 single-rooted bovine extracted teeth were filled with 0.01% methylene blue and submitted to photodynamic therapy for 90seconds. After that, the roots were divided into six groups (n=10) according to the final irrigation protocol: distilled water (DW), DW+US, 17% EDTA, QMix, EDTA+US, and QMix+US...
January 5, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28061512/assessment-of-rod-cone-and-intrinsically-photosensitive-retinal-ganglion-cell-contributions-to-the-canine-chromatic-pupillary-response
#17
Connie Y Yeh, Kristin L Koehl, Christine D Harman, Simone Iwabe, José M Guzman, Simon M Petersen-Jones, Randy H Kardon, András M Komáromy
Purpose: The purpose of this study was to evaluate a chromatic pupillometry protocol for specific functional assessment of rods, cones, and intrinsically photosensitive retinal ganglion cells (ipRGCs) in dogs. Methods: Chromatic pupillometry was tested and compared in 37 dogs in different stages of primary loss of rod, cone, and combined rod/cone and optic nerve function, and in 5 wild-type (WT) dogs. Eyes were stimulated with 1-s flashes of dim (1 cd/m2) and bright (400 cd/m2) blue light (for scotopic conditions) or bright red (400 cd/m2) light with 25-cd/m2 blue background (for photopic conditions)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28058292/monoclonal-antibodies-that-recognize-various-folding-states-of-pure-human-butyrylcholinesterase-can-immunopurify-butyrylcholinesterase-from-human-plasma-stored-at-elevated-temperatures
#18
Hong Peng, Thomas A Blake, Rudolph C Johnson, Alicia J Dafferner, Stephen Brimijoin, Oksana Lockridge
Human plasma to be analyzed for exposure to cholinesterase inhibitors is stored at 4 °C or lower to prevent denaturation of human butyrylcholinesterase (HuBChE), the biomarker of exposure. Currently published protocols immunopurify HuBChE using antibodies that bind native HuBChE before analysis by mass spectrometry. It is anticipated that the plasma collected from human casualties may be stored nonideally at elevated temperatures of up to 45 °C for days or maybe weeks. At 45 °C, the plasma loses 50% of its HuBChE activity in 8 days and 95% in 40 days...
December 31, 2016: ACS Omega
https://www.readbyqxmd.com/read/28052116/functional-maturation-of-human-stem-cell-derived-neurons-in-long-term-cultures
#19
Rebecca S Lam, Felix M Töpfer, Phillip G Wood, Volker Busskamp, Ernst Bamberg
Differentiated neurons can be rapidly acquired, within days, by inducing stem cells to express neurogenic transcription factors. We developed a protocol to maintain long-term cultures of human neurons, called iNGNs, which are obtained by inducing Neurogenin-1 and Neurogenin-2 expression in induced pluripotent stem cells. We followed the functional development of iNGNs over months and they showed many hallmark properties for neuronal maturation, including robust electrical and synaptic activity. Using iNGNs expressing a variant of channelrhodopsin-2, called CatCh, we could control iNGN activity with blue light stimulation...
2017: PloS One
https://www.readbyqxmd.com/read/28050362/bio-medical-waste-managment-in-a-tertiary-care-hospital-an-overview
#20
Anita Pandey, Sanjiv Ahuja, Molly Madan, Ajay Kumar Asthana
INTRODUCTION: Bio-Medical Waste (BMW) management is of utmost importance as its improper management poses serious threat to health care workers, waste handlers, patients, care givers, community and finally the environment. Simultaneously, the health care providers should know the quantity of waste generated in their facility and try to reduce the waste generation in day-to-day work because lesser amount of BMW means a lesser burden on waste disposal work and cost saving. AIM: To have an overview of management of BMW in a tertiary care teaching hospital so that effective interventions and implementations can be carried out for better outcome...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
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