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https://www.readbyqxmd.com/read/28721010/antiglioma-effects-of-cytarabine-on-leptomeningeal-metastasis-of-high-grade-glioma-by-targeting-the-pi3k-akt-mtor-pathway
#1
Kai-Hong Zhao, Can Zhang, Yue Bai, Yan Li, Xun Kang, Jian-Xin Chen, Kun Yao, Tao Jiang, Xiao-Song Zhong, Wen-Bin Li
Leptomeningeal metastasis (LM) of high-grade glioma is a highly lethal disease requiring new effective therapeutic measures. For both de novo or relapsed glioma with LM, intrathecal cytarabine chemotherapy is not frequently used for first-line and relapse protocols. We encountered a clinical case demonstrating effective application of cytarabine in high-grade glioma with LM, prompting us to explore the effects of cytarabine on malignant glioma and molecular mechanisms of such effects through in vivo and in vitro experiments...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28713887/morphology-dependent-catalysis-and-surface-enhanced-raman-scattering-sers-studies-using-pd-nanostructures-in-dna-ctab-and-pva-scaffolds
#2
Subrata Kundu, Su-In Yi, Lian Ma, Yunyun Chen, Wei Dai, Alexander M Sinyukov, Hong Liang
Palladium nanoparticles (Pd NPs) of three different morphologies viz., nanocubes with cetyltrimethylammonium bromide (CTAB), nanowires with polyvinyl alcohol (PVA) and Pd NPs with deoxyribonucleic acid (DNA) scaffolds were synthesized by UV-irradiation. Catalysis and surface enhanced Raman scattering (SERS) studies were done with the synthesized morphologically distinct Pd nanostructures for the very first time. The catalytic rate was extremely high with Pd nanowires templated with PVA and the order of the catalytic rate was Pd nanowires in PVA > Pd nanocubes in CTAB > DNA-Pd wire-like assemblies...
July 17, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28713831/new-mydriasis-free-electroretinogram-recorded-with-skin-electrodes-in-healthy-subjects
#3
Ken Asakawa, Kana Amino, Machiho Iwase, Yuki Kusayanagi, Akiho Nakamura, Rio Suzuki, Takashi Yuuki, Hitoshi Ishikawa
PURPOSE: To evaluate the reproducibility and consistency of the new mydriasis-free electroretinogram (ERG) with a skin electrode (RETeval) device, to determine the normative values of parameters, and to clarify the usefulness of pupil records to colored-light stimulus. METHODS: A total of 100 eyes of 50 healthy subjects (mean age, 21.4 years) were enrolled. The diagnostic parameters obtained by the RETeval device were examined under the following conditions. The reproducibility was determined with the coefficient of variation (CV)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28703245/blue-light-promoted-carbon-carbon-double-bond-isomerization-and-its-application-in-the-syntheses-of-quinolines
#4
Xinzheng Chen, Shuxian Qiu, Sasa Wang, Huifei Wang, Hongbin Zhai
A blue-light-promoted carbon-carbon double bond isomerization in the absence of any photoredox catalyst is reported. It provides rapid access to a series of quinolines in good to excellent yields under simple aerobic conditions. The protocol is direct, catalyst-free and operationally convenient.
July 13, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28698267/clinical-criteria-for-tracheostomy-decannulation-in-subjects-with-acquired-brain-injury
#5
Claudia Enrichi, Irene Battel, Cristiano Zanetti, Isabella Koch, Laura Ventura, Katie Palmer, Francesca Meneghello, Francesco Piccione, Simonetta Rossi, Marta Lazzeri, Maurizio Sommariva, Andrea Turolla
BACKGROUND: Patients with acquired brain injury (ABI) often require long periods of having a tracheostomy tube for airway protection and prolonged mechanical ventilation. It has been recognized that fast and safe decannulation improves outcomes and facilitates the recovery process. Nevertheless, few studies have provided evidence for decannulation criteria, despite the high prevalence of ABI subjects with tracheostomies. The aim of our study was to assess which clinical parameters are the best predictors for decannulation in subjects with ABI...
July 11, 2017: Respiratory Care
https://www.readbyqxmd.com/read/28685876/eus-fna-of-the-merkel-cell-carcinoma-metastasis-to-the-pancreas-cytomorphology-and-immunocytochemistry-on-direct-cytological-smears
#6
T Stoos-Veic, M Tadic, G Aralica, V Milicic, C Tomasovic-Loncaric
OBJECTIVE: To report two cases of Merkel cell carcinoma (MCC) metastatic to the pancreas diagnosed with endoscopic ultrasound-guided-fine needle aspiration (EUS-FNA) and to add the case of concomitant chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL) and MCC to the literature. The aim is to alert the cytopathologists once more to the problems of differential diagnosis of pancreatic metastasis of MCC and to describe the possibilities of ancillary methods performed on direct cytological smears...
July 6, 2017: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/28677080/comparison-of-impact-of-two-decontamination-solutions-on-the-viability-of-the-cells-in-human-amnion
#7
Ingrida Smeringaiova, Peter Trosan, Miluse Berka Mrstinova, Jan Matecha, Jan Burkert, Jan Bednar, Katerina Jirsova
Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this study, we tested two decontamination solutions, commercial (BASE·128) and laboratory decontamination solution (LDS), with an analogous content of antimycotic/antibiotics for their cytotoxic effect on HAM epithelial (EC) and mesenchymal stromal cells (MSC)...
July 4, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28667624/blue-native-page-analysis-of-bacterial-secretion-complexes
#8
Susann Zilkenat, Tobias Dietsche, Julia V Monjarás Feria, Claudia E Torres-Vargas, Mehari Tesfazgi Mebrhatu, Samuel Wagner
Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpression, purification, and biochemical characterization of these complexes is often difficult, impeding progress in understanding the structure and function of these systems. Blue native (BN) polyacrylamide gel electrophoresis (PAGE) allows for the investigation of these transmembrane complexes right from their originating membranes, without the need for long preparative steps, and is amenable to the parallel characterization of a number of samples under near-native conditions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28647284/the-effects-of-high-intensity-interval-training-on-muscle-size-and-quality-in-overweight-and-obese-adults
#9
Malia N M Blue, Abbie E Smith-Ryan, Eric T Trexler, Katie R Hirsch
OBJECTIVES: Despite growing popularity of high intensity interval training (HIIT) for improving health and fitness, limited data exist identifying the effects of HIIT on muscle characteristics. The purpose of the current study was to investigate the effects of a 3-week HIIT intervention on muscle size and quality in overweight and obese men and women. DESIGN: Randomized controlled trial. METHODS: Forty-four overweight and obese men and women (mean±SD; age: 35...
June 8, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28636921/characterizing-intracellular-ice-formation-of%C3%A2-lymphoblasts-using-low-temperature-raman%C3%A2-spectroscopy
#10
Guanglin Yu, Yan Rou Yap, Kathryn Pollock, Allison Hubel
Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions. The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments. Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death. The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28632841/green-light-autofluorescence-versus-combined-blue-light-autofluorescence-and-near-infrared-reflectance-imaging-in-geographic-atrophy-secondary-to-age-related-macular-degeneration
#11
COMPARATIVE STUDY
Maximilian Pfau, Lukas Goerdt, Steffen Schmitz-Valckenberg, Matthias M Mauschitz, Divyansh K Mishra, Frank G Holz, Moritz Lindner, Monika Fleckenstein
Purpose: To compare the intermodality and interreader agreement for geographic atrophy (GA) lesion size quantification in green-light fundus autofluorescence (GAF; excitation = 518 nm) versus combined blue-light fundus autofluorescence (BAF; excitation = 488 nm) and near-infrared reflectance (NIR; 820 nm) -based grading. Methods: Confocal scanning laser ophthalmoscopy (cSLO) GAF, BAF, and NIR images of 40 eyes from 29 patients (mean age 79.7 years) with GA secondary to AMD were recorded according to a standardized protocol...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28624146/the-impact-of-the-blue-protocol-ultrasonography-on-the-time-taken-to-treat-acute-respiratory-distress-in-the-emergency-department
#12
Javad Seyedhosseini, Golnaz Bashizadeh-Fakhar, Shirani Farzaneh, Mehdi Momeni, Ehsan Karimialavijeh
No abstract text is available yet for this article.
June 6, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28622352/volunteer-trials-of-a-novel-improvised-dry-decontamination-protocol-for-use-during-mass-casualty-incidents-as-part-of-the-uk-s-initial-operational-response-ior
#13
Richard Amlôt, Holly Carter, Lorna Riddle, Joanne Larner, Robert P Chilcott
Previous studies have demonstrated that rapid evacuation, disrobing and emergency decontamination can enhance the ability of emergency services and acute hospitals to effectively manage chemically-contaminated casualties. The purpose of this human volunteer study was to further optimise such an "Initial Operational Response" by (1) identifying an appropriate method for performing improvised skin decontamination and (2) providing guidance for use by first responders and casualties. The study was performed using two readily available, absorbent materials (paper towels and incontinence pads)...
2017: PloS One
https://www.readbyqxmd.com/read/28620429/novel-approaches-to-ultrasonography-of-the-lung-and-pleural-space-where-are-we-now
#14
REVIEW
Daniel Lichtenstein
This review article is an update of what should be known for practicing basic lung ultrasound in the critically ill (LUCI) and is also of interest for less critical disciplines (e.g. pulmonology). It pinpoints on the necessity of a professional machine (not necessarily a sophisticated one) and probe. It lists the 10 main signs of LUCI and some of the main protocols made possible using LUCI: the BLUE protocol for a respiratory failure, the FALLS protocol for a circulatory failure, the SESAME protocol for a cardiac arrest and the investigation of a ventilated acute respiratory distress syndrome patient, etc...
June 2017: Breathe
https://www.readbyqxmd.com/read/28619615/evaluation-of-antimicrobial-activity-of-association-of-chlorhexidine-to-photosensitizer-used-in-photodynamic-therapy-in-root-canals-infected-by-enterococcus-faecalis
#15
Matheus Albino Souza, Guilherme Lima, Bianca Pazinatto, Karolina Frick Bischoff, Huriel Scartazzini Palhano, Doglas Cecchin
INTRODUCTION: The aim of the present study was to evaluate, in vitro, the influence of the addition of chlorhexidine to photosensitiser in the antimicrobial activity of photodynamic therapy in root canals infected by Enterococcus faecalis. METHODS: the root canals of 50 single-rooted human extracted teeth were enlarged up to a file F3 of Pro-Taper system, autoclaved, inoculated with Enterococcus faecalis and incubated for 14 days. The samples were divided into five groups (n=10) according to the protocol of decontamination: G1 (control group) - no procedure was performed; G2-photosensitiser (0...
June 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28615276/bluehealth-a-study-programme-protocol-for-mapping-and-quantifying-the-potential-benefits-to-public-health-and-well-being-from-europe-s-blue-spaces
#16
James Grellier, Mathew P White, Maria Albin, Simon Bell, Lewis R Elliott, Mireia Gascón, Silvio Gualdi, Laura Mancini, Mark J Nieuwenhuijsen, Denis A Sarigiannis, Matilda van den Bosch, Tanja Wolf, Susanne Wuijts, Lora E Fleming
INTRODUCTION: Proximity and access to water have long been central to human culture and accordingly deliver countless societal benefits. Over 200 million people live on Europe's coastline, and aquatic environments are the top recreational destination in the region. In terms of public health, interactions with 'blue space' (eg, coasts, rivers, lakes) are often considered solely in terms of risk (eg, drowning, microbial pollution). Exposure to blue space can, however, promote health and well-being and prevent disease, although underlying mechanisms are poorly understood...
June 14, 2017: BMJ Open
https://www.readbyqxmd.com/read/28608220/calpain-zymography-general-methodology-and-protocol
#17
Kevin K W Wang
Casein zymography has become one of the gold standard assays for monitoring mammalian calcium-activated proteases (calpains) in purified enzyme, cell, or tissue samples. This calpain zymography method takes advantages of (1) casein is an excellent substrate for major isoforms of calpains (Calpain-1, 2 and 3), (2) the embedded casein is digested into small peptides where the calpain bands are located, thus creating a clear zone upon Commassie blue gel staining, and (3) the calpain isoforms have different gel mobility under native gel conditions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28608215/zymography-in-multiwells-for-quality-assessment-of-proteinases
#18
Ambili Mechoor, Madathiparambil G Madanan
Zymography is a well-standardized protocol for the qualitative assessment and analysis of proteinases under specified conditions. However, analysis of a large number of samples simultaneously becomes a challenge when the zymography is carried out by the usual protocol of electrophoresis. This can be overcome by assaying the matrix-degrading proteinases in substrate-impregnated gels in multiwells. Enzymes are copolymerized with 300 mL of 10% acrylamide impregnated with gelatin substrate and incubated for 16 h...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28608200/mmp-activity-detection-in-zymograms
#19
Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska, Gergő Szűcs, Péter Ferdinandy
Matrix metalloproteinases (MMP) belong to a distinguished class of zinc-dependent endopeptidases. Zymography is a semi-quantitative tool for determining the activity of different MMP isoenzymes in a variety of biological samples. In substrate gel zymography, protein samples of different origin (tissue, cell lysates, plasma/serum, perfusates, other liquids) are separated in sodium dodecyl sulfate (SDS) polyacrylamide gels containing copolymerized substrate (gelatin, casein, elastin, etc.), and after incubation-enabling substrate cleavage by MMPs, MMP activities are detected after the gel staining as transparent bands against a dark-blue background...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28540349/dataset-of-protein-species-from-human-liver
#20
Stanislav Naryzhny, Maria Maynskova, Victor Zgoda, Alexander Archakov
This article contains data related to the research article entitled "Zipf׳s law in proteomics" (Naryzhny et al., 2017) [1]. The protein composition in the human liver or hepatocarcinoma (HepG2) cells extracts was estimated using a filter-aided sample preparation (FASP) protocol. The protein species/proteoform composition in the human liver was determined by two-dimensional electrophoresis (2-DE) followed by Electrospray Ionization Liquid Chromatography-Tandem Mass Spectrometry (ESI LC-MS/MS). In the case of two-dimensional electrophoresis (2-DE), the gel was stained with Coomassie Brilliant Blue R350, and image analysis was performed with ImageMaster 2D Platinum software (GE Healthcare)...
June 2017: Data in Brief
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