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https://www.readbyqxmd.com/read/28072432/closed-vitrification-system-as-a-platform-for-cryopreservation-of-tissue-engineered-constructs
#1
N A Trufanova, V S Zaikov, A V Zinchenko, A Yu Petrenko, Y A Petrenko
BACKGROUND: Cryopreservation of mesenchymal stromal cells (MSCs) and MSCs-based tissue engineered constructs (TECs) is a promising strategy for regenerative medicine. OBJECTIVE: To examine vitrification system consisting of multicomponent vitreous solution, closed type container, human adult MSCs and two-step exposure procedure as a platform for cryopreservation of MSCs-based TECs. MATERIALS AND METHODS: Vitrification properties of solutions were studied by visual analysis and calorimetry...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28065860/influence-of-ultrasonic-activation-over-final-irrigants-in-the-removal-of-photosensitizer-from-root-canal-walls-after-photodynamic-therapy
#2
Matheus Albino Souza, Bianca Pazinatto, Karolina Frick Bischoff, Huriel Scartazzini Palhano, Doglas Cecchin, José Antônio Poli de Figueiredo
INTRODUCTION: The aim of the present study was to evaluate the influence of ultrasonic activation (US) of the final irrigant in the removal of the photosensitizer from root canal walls after photodynamic therapy. METHODS: The root canals of 60 single-rooted bovine extracted teeth were filled with 0.01% methylene blue and submitted to photodynamic therapy for 90seconds. After that, the roots were divided into six groups (n=10) according to the final irrigation protocol: distilled water (DW), DW+US, 17% EDTA, QMix, EDTA+US, and QMix+US...
January 5, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28061512/assessment-of-rod-cone-and-intrinsically-photosensitive-retinal-ganglion-cell-contributions-to-the-canine-chromatic-pupillary-response
#3
Connie Y Yeh, Kristin L Koehl, Christine D Harman, Simone Iwabe, José M Guzman, Simon M Petersen-Jones, Randy H Kardon, András M Komáromy
Purpose: The purpose of this study was to evaluate a chromatic pupillometry protocol for specific functional assessment of rods, cones, and intrinsically photosensitive retinal ganglion cells (ipRGCs) in dogs. Methods: Chromatic pupillometry was tested and compared in 37 dogs in different stages of primary loss of rod, cone, and combined rod/cone and optic nerve function, and in 5 wild-type (WT) dogs. Eyes were stimulated with 1-s flashes of dim (1 cd/m2) and bright (400 cd/m2) blue light (for scotopic conditions) or bright red (400 cd/m2) light with 25-cd/m2 blue background (for photopic conditions)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28058292/monoclonal-antibodies-that-recognize-various-folding-states-of-pure-human-butyrylcholinesterase-can-immunopurify-butyrylcholinesterase-from-human-plasma-stored-at-elevated-temperatures
#4
Hong Peng, Thomas A Blake, Rudolph C Johnson, Alicia J Dafferner, Stephen Brimijoin, Oksana Lockridge
Human plasma to be analyzed for exposure to cholinesterase inhibitors is stored at 4 °C or lower to prevent denaturation of human butyrylcholinesterase (HuBChE), the biomarker of exposure. Currently published protocols immunopurify HuBChE using antibodies that bind native HuBChE before analysis by mass spectrometry. It is anticipated that the plasma collected from human casualties may be stored nonideally at elevated temperatures of up to 45 °C for days or maybe weeks. At 45 °C, the plasma loses 50% of its HuBChE activity in 8 days and 95% in 40 days...
December 31, 2016: ACS Omega
https://www.readbyqxmd.com/read/28052116/functional-maturation-of-human-stem-cell-derived-neurons-in-long-term-cultures
#5
Rebecca S Lam, Felix M Töpfer, Phillip G Wood, Volker Busskamp, Ernst Bamberg
Differentiated neurons can be rapidly acquired, within days, by inducing stem cells to express neurogenic transcription factors. We developed a protocol to maintain long-term cultures of human neurons, called iNGNs, which are obtained by inducing Neurogenin-1 and Neurogenin-2 expression in induced pluripotent stem cells. We followed the functional development of iNGNs over months and they showed many hallmark properties for neuronal maturation, including robust electrical and synaptic activity. Using iNGNs expressing a variant of channelrhodopsin-2, called CatCh, we could control iNGN activity with blue light stimulation...
2017: PloS One
https://www.readbyqxmd.com/read/28050362/bio-medical-waste-managment-in-a-tertiary-care-hospital-an-overview
#6
Anita Pandey, Sanjiv Ahuja, Molly Madan, Ajay Kumar Asthana
INTRODUCTION: Bio-Medical Waste (BMW) management is of utmost importance as its improper management poses serious threat to health care workers, waste handlers, patients, care givers, community and finally the environment. Simultaneously, the health care providers should know the quantity of waste generated in their facility and try to reduce the waste generation in day-to-day work because lesser amount of BMW means a lesser burden on waste disposal work and cost saving. AIM: To have an overview of management of BMW in a tertiary care teaching hospital so that effective interventions and implementations can be carried out for better outcome...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28042323/microbubble-gas-volume-a-unifying-dose-parameter-in-blood-brain-barrier-opening-by-focused-ultrasound
#7
Kang-Ho Song, Alexander C Fan, Joshua J Hinkle, Joshua Newman, Mark A Borden, Brandon K Harvey
Focused ultrasound with microbubbles is being developed to transiently, locally and noninvasively open the blood-brain barrier (BBB) for improved pharmaceutical delivery. Prior work has demonstrated that, for a given concentration dose, microbubble size affects both the intravascular circulation persistence and extent of BBB opening. When matched to gas volume dose, however, the circulation half-life was found to be independent of microbubble size. In order to determine whether this holds true for BBB opening as well, we independently measured the effects of microbubble size (2 vs...
2017: Theranostics
https://www.readbyqxmd.com/read/28039000/moving-into-advanced-nanomaterials-toxicity-of-rutile-tio2-nanoparticles-immobilized-in-nanokaolin-nanocomposites-on-hepg2-cell-line
#8
Maria João Bessa, Carla Costa, Julian Reinosa, Cristiana Pereira, Sónia Fraga, José Fernández, Miguel A Bañares, João Paulo Teixeira
Immobilization of nanoparticles on inorganic supports has been recently developed, resulting in the creation of nanocomposites. Concerning titanium dioxide nanoparticles (TiO2 NPs(1)), these have already been developed in conjugation with clays, but so far there are no available toxicological studies on these nanocomposites. The present work intended to evaluate the hepatic toxicity of nanocomposites (C-TiO2(2)), constituted by rutile TiO2 NPs immobilized in nanokaolin (NK(3)) clay, and its individual components...
December 28, 2016: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28032356/use-of-computer-assisted-image-analysis-for-semi-quantitative-histology-of-the-hepatopancreas-in-whiteleg-shrimp-penaeus-vannamei-boone
#9
F Cervellione, C McGurk, T Berger Eriksen, W Van den Broeck
Despite the increasing use of novel molecular techniques in pathology, histology remains the standard method for monitoring tissue alterations and for assessing pathology. Histopathological evaluation is generally laborious and subjective with risk of discrepancies in semi-quantitative scoring between pathologists. In contrast, computer-assisted image analysis (CAIA) is potentially faster, more objective and thus suitable for routine screening. Limited research has been carried out on CAIA in crustacean histopathology, and the methods described were not fully automated...
December 29, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28028943/the-effects-of-anti-cd3-cd28-coated-beads-and-il-2-on-expanded-t-cell-for-immunotherapy
#10
Sirimarn Martkamchan, Nattawat Onlamoon, Siyu Wang, Kovit Pattanapanyasat, Palanee Ammaranond
BACKGROUND: The activation of peripheral blood mononucleated cells (PBMCs) with anti-CD3/CD28-coated magnetic beads promotes intrinsic resistance to HIV as well as cell expansion. OBJECTIVES: The aim of this study was to define an optimal cell isolation protocol for the expansion of PBMCs using anti-CD3/CD28-coated bead stimulation, with the ultimate goal of using these cells for adoptive therapy. MATERIAL AND METHODS: PBMCs were isolated from healthy donor blood samples...
September 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28013442/dmso-free-cryopreservation-of-adipose-derived-mesenchymal-stromal-cells-expansion-medium-affects-post-thaw-survival
#11
Olena Rogulska, Yuri Petrenko, Alexander Petrenko
Off-the-shelf availability of human adipose-derived mesenchymal stromal cells (ASCs) for regenerative medicine application requires the development of nontoxic, safe, and efficient protocols for cryopreservation. Favorably, such cell processing protocols should not contain xenogeneic or toxic components, such as fetal bovine serum (FS) and dimethyl sulfoxide (DMSO). The objective of the study was to assess the sensitivity of ASCs to DMSO-free cryopreservation protocol depending on their expansion conditions: conventional, based on the application of FS or xeno-free, using PL as a medium supplement...
December 24, 2016: Cytotechnology
https://www.readbyqxmd.com/read/28012901/pravastatin-and-simvastatin-inhibit-the-adhesion-replication-and-proliferation-of-toxoplasma-gondii-rh-strain-in-hela-cells
#12
Raquel Arruda Sanfelice, Suelen Santos da Silva, Larissa Rodrigues Bosqui, Milena Menegazzo Miranda-Sapla, Bellisa Freitas Barbosa, Rafaela José Silva, Eloísa A Vieira Ferro, Luciano Aparecido Panagio, Italmar Teodorico Navarro, Juliano Bordignon, Ivete Conchon-Costa, Wander Rogerio Pavanelli, Ricardo Sergio Almeida, Idessania Nazareth Costa
The conventional treatment for toxoplasmosis with pyrimethamine and sulfadiazine shows toxic effects to the host, and it is therefore necessary to search for new drugs. Some studies suggest the use of statins, which inhibit cholesterol synthesis in humans and also the initial processes of isoprenoid biosynthesis in the parasite. Thus, the objective of this study was to evaluate the activity of the statins pravastatin and simvastatin in HeLa cells infected in vitro with the RH strain of T. gondii. HeLa cells (1×10(5)) were infected with T...
December 21, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27992951/limits-on-carbon-sequestration-in-arid-blue-carbon-ecosystems
#13
Lisa M Schile, J Boone Kauffman, Stephen Crooks, James W Fourqurean, Jane Glavan, J Patrick Megonigal
Coastal ecosystems produce and sequester significant amounts of carbon ('blue carbon'), which has been well documented in humid and semi-humid regions of temperate and tropical climates but less so in arid regions where mangroves, marshes, and seagrasses exist near the limit of their tolerance for extreme temperature and salinity. To better understand these unique systems, we measured whole-ecosystem carbon stocks in 58 sites across the United Arab Emirates in natural and planted mangroves, salt marshes, seagrass beds, microbial mats, and coastal sabkha (inter- and supratidal unvegetated salt flats)...
December 19, 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/27991773/all-solution-based-aggregation-control-in-solid-state-photon-upconverting-organic-model-composites
#14
Hossein Goudarzi, Panagiotis E Keivanidis
Hitherto, great strides have been made in the development of organic systems that exhibit triplet-triplet annihilation-induced photon-energy upconversion (TTA-UC). Yet, the exact role of intermolecular states in solid-state TTA-UC composites remains elusive. Here we perform a comprehensive spectroscopic study in a series of solution-processable solid-state TTA-UC organic composites with increasing segregated phase content for elucidating the impact of aggregate formation in their TTA-UC properties. Six different states of aggregation are reached in composites of the 9,10-diphenylanthracene (DPA) blue emitter mixed with the (2,3,7,8,12,13,17,18-octaethylporphyrinato)platinum(II) sensitizer (PtOEP) in a fixed nominal ratio (2 wt % PtOEP)...
December 19, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27987452/colorimetric-detection-and-removal-of-radioactive-co-ions-using-sodium-alginate-based-composite-beads
#15
Daigeun Kim, Ara Jo, Hee-Man Yang, Bum-Kyoung Seo, Kune-Woo Lee, Taek Seung Lee
We demonstrate a simple method for the visual determination and removal of Co ions using a bead-shaped, capturing probe based on hybridized sodium alginate. For Co ions, the designed protocol consisted of three main constituents: an azopyridine-based Co ion-probe for visual detection; sodium alginate as an adsorbent for the Co ion and a bead construct for removal and structure; silica as a linker for the probe and the alginate, leading to a robust structure. When the composite beads were exposed to Co ions, the yellow color of the beads turned to intensive violet and the color intensity was associated with the Co ion concentration...
December 5, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27987046/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-identification-of-micro-organisms-in-the-routine-clinical-microbiology-laboratory
#16
C Wattal, J K Oberoi, N Goel, R Raveendran, S Khanna
The study evaluates the utility of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) Vitek MS for identification of microorganisms in the routine clinical microbiology laboratory. From May 2013 to April 2014, microbial isolates recovered from various clinical samples were identified by Vitek MS. In case of failure to identify by Vitek MS, the isolate was identified using the Vitek 2 system (bioMerieux, France) and serotyping wherever applicable or otherwise by nucleic acid-mediated methods...
December 16, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27959574/porcine-dorsal-root-ganglia-ovarian-neurons-are-affected-by-long-lasting-testosterone-treatment
#17
B Jana, K Palus, K Meller, J Całka
We studied the effect of testosterone overdose on the number, distribution and chemical coding of ovarian neurons in the dorsal root ganglia (DRGs) in pigs. On day 3 of the estrous cycle, the ovaries of both the control and experimental gilts were injected with retrograde tracer Fast Blue. From day 4 of the estrous cycle to the expected day 20 of the second studied cycle, the experimental gilts were injected with testosterone, while the control gilts received oil. After the completion of the protocol the Th16-L5 DRGs were collected...
December 13, 2016: Physiological Research
https://www.readbyqxmd.com/read/27941596/chlorophyll-a-estimation-around-the-antarctica-peninsula-using-satellite-algorithms-hints-from-field-water-leaving-reflectance
#18
Chen Zeng, Huiping Xu, Andrew M Fischer
Ocean color remote sensing significantly contributes to our understanding of phytoplankton distribution and abundance and primary productivity in the Southern Ocean (SO). However, the current SO in situ optical database is still insufficient and unevenly distributed. This limits the ability to produce robust and accurate measurements of satellite-based chlorophyll. Based on data collected on cruises around the Antarctica Peninsula (AP) on January 2014 and 2016, this research intends to enhance our knowledge of SO water and atmospheric optical characteristics and address satellite algorithm deficiency of ocean color products...
December 7, 2016: Sensors
https://www.readbyqxmd.com/read/27940236/engineering-anatase-hierarchically-cactus-like-tio2-arrays-for-photoelectrochemical-and-visualized-sensing-platform
#19
Chaomin Gao, Yanhu Wang, Shuai Yuan, Jie Xue, Bingqiang Cao, Jinghua Yu
This work described that one-step synthesis three dimensional anatase hierarchically cactus-like TiO2 arrays (AHCT) and their application in constructing a novel photoelectrochemical (PEC) and visualized sensing platform based on molecular imprinting technique, which reports its result with the prussian blue (PB) electrode served as the electrochromic indicator for the detection of glycoprotein (RNase B). The AHCT arrays were perpendicularly grown on FTO substrate with tunable sizes, offering many advantages, such as large contact area, rapid charge electron separation and transport...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27939963/introducing-euro-glo-a-rare-earth-metal-chelate-with-numerous-applications-for-the-fluorescent-localization-of-myelin-and-amyloid-plaques-in-brain-tissue-sections
#20
Larry Schmued, James Raymick
BACKGROUND: The vast majority of fluorochromes are organic in nature and none of the few existing chelates have been applied as histological tracers for localizing brain anatomy and pathology. NEW METHOD: In this study we have developed and characterized a Europium chelate with the ability to fluorescently label normal and pathological myelin in control and toxicant-exposed rats, as well as the amyloid plaques in aged AD/Tg mice. RESULTS: This study demonstrates how Euro-Glo can be used for the detailed labeling of both normal myelination in the control rat as well as myelin pathology in the kainic acid exposed rat...
December 8, 2016: Journal of Neuroscience Methods
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