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Blue protocol

Sachin Kumar, Marie-Dominique Filippi
Transmission electron microscopy (TEM) provides details of the cellular organization and ultrastructure. However, TEM analysis of rare cell populations, especially cells in suspension such as hematopoietic stem cells (HSCs), remains limited due to the requirement of a high cell number during sample preparation. There are a few cytospin or monolayer approaches for TEM analysis from scarce samples, but these approaches fail to get significant quantitative data from the limited number of cells. Here, an alternative and novel approach for sample preparation in TEM studies is described for rare cell populations that enables quantitative analysis...
October 12, 2016: Journal of Visualized Experiments: JoVE
Miguel José Francisco, Antonio Rahal, Fabio Augusto Cardillo Vieira, Paulo Savoia Dias da Silva, Marcelo Buarque de Gusmão Funari
Ultrasound examination of the chest has advanced in recent decades. This imaging modality is currently used to diagnose several pathological conditions and provides qualitative and quantitative information. Acoustic barriers represented by the aerated lungs and the bony framework of the chest generate well-described sonographic artifacts that can be used as diagnostic aids. The normal pleural line and A, B, C, E and Z lines (also known as false B lines) are artifacts with specific characteristics. Lung consolidation and pneumothorax sonographic patterns are also well established...
July 2016: Einstein
Derya İlem-Özdemir, Ulkem Yararbas, Baha Zengel, Gökhan Ertan, Makbule Asikoglu
The sentinel lymph node (SLN) is defined as the first regional lymph node to receive lymphatic drainage from a malignant tumor. Therefore, this node is a "sentinel" for second metastatic lymph node stations and for labeling regional tumor spread. For SLN detection, many surgeons preferred a combination of a preoperative injection of radiolabeled colloid and the intraoperative injection of blue dye. Under this combination protocol, nodes are considered to be "sentinel nodes" if they are radioactive and blue...
October 11, 2016: Applied Radiation and Isotopes
A Krigel, M Berdugo, E Picard, R Levy-Boukris, I Jaadane, L Jonet, M Dernigoghossian, C Andrieu-Soler, A Torriglia, F Behar-Cohen
To save energy, the European directives from the Eco-design of Energy Using Products (2005/32/CE) has recommended the replacement of incandescent lamps by more economic devices such as Light Emitting Diodes (LEDs). However, the emission spectrum of these devices is enriched in blue radiations, known to be potentially dangerous to the retina. Recent studies showed that light exposure contribute to the onset of early stages of Age related macular degeneration (AMD). Here, we investigate, in albinos and pigmented rats, the effects of different exposure protocols...
October 14, 2016: Neuroscience
Andrew A M Ibey, Derek Andrews, Barb Ferreira
The authors present a case in which a physical anomaly with an infusion pump resulted in an unforeseen fault that the nurse's attempts to resolve unknowingly exacerbated. This case study presents the first report in the literature to detail the difficulty in recreating a patient safety event using smart pump logs, support server continuous quality improvement (CQI) data, and the drug order entry system to elucidate the clinical scenario. A 75-year-old male patient presented to a major teaching hospital and was admitted to the intensive care unit (ICU) with a massive gastrointestinal bleed and myocardial infarction, then stabilized...
December 2016: Drug Saf Case Rep
N A Jarvis, J R Donaldson, C A O'Bryan, S C Ricke, P G Crandall
Listeria monocytogenes can be carried by and infect poultry, although the clinical disease in birds is rare. Escape from macrophage phagocytosis is a key step in pathogenesis for L. monocytogenes Therefore, we investigated the infection of the chicken macrophage-like cell line HD11 with 2 strains of L. monocytogenes EGD-e and Scott A. After infection, L. monocytogenes was quantified by spread plating and HD11 was quantified with trypan blue exclusion stain before enumeration. The standard macrophage killing protocols require washing the cell monolayers 3 times with PBS, which was found to negatively influence HD11 monolayers...
October 12, 2016: Poultry Science
Serban San-Marina, Ayushman Sharma, Stephen G Voss, Jeffrey R Janus, Grant S Hamilton
Importance: Nasal reconstruction in patients who are missing a significant amount of structural nasal support remains a difficult challenge. One challenge is the deficiency of cartilage left within the nose as a consequence of rhinectomy or a midline destructive disease. Historically, the standard donor source for large quantities of native cartilage has been costal cartilage. Objective: To enable the development of protocols for new mesenchymal stem cell technologies as alternative procedures with reduced donor site morbidity, risk of infection and extrusion...
October 13, 2016: JAMA Facial Plastic Surgery
Agnes Afrodite S Albuquerque, Edson A Margarido, Antonio Carlos Menardi, Adilson Scorzoni, Andrea Carla Celotto, Alfredo J Rodrigues, Walter Vilella A Vicente, Paulo Roberto B Evora
Objective: To examine if methylene blue (MB) can counteract or prevent protamine (P) cardiovascular effects. Methods: The protocol included five heparinized pig groups: Group Sham -without any drug; Group MB - MB 3 mg/kg infusion; Group P - protamine; Group P/MB - MB after protamine; Group MB/P - MB before protamine. Nitric oxide levels were obtained by the nitric oxide/ozone chemiluminescence method, performed using the Nitric Oxide Analizer 280i (Sievers, Boulder, CO, USA)...
May 2016: Brazilian Journal of Cardiovascular Surgery
Hong-Yan Zhuo, Hai-Feng Su, Zhao-Zhen Cao, Wei Liu, Shu-Ao Wang, Lei Feng, Gui-Lin Zhuang, Shui-Chao Lin, Mohamedally Kurmoo, Chen-Ho Tung, Di Sun, Lan-Sun Zheng
Cu(CF3 COO)2 reacts with tert-butylacetylene (tBuC≡CH) in methanol in the presence of metallic copper powder to give two air-stable clusters, [Cu(I)15 (tBuC≡C)10 (CF3 COO)5 ]⋅tBuC≡CH (1) and [Cu(I)16 (tBuC≡C)12 (CF3 COO)4 (CH3 OH)2 ] (2). The assembly process involves in situ comproportionation reaction between Cu(2+) and Cu(0) and the formation of two different clusters is controlled by reactants concentration. The clusters consist of Cu15 and Cu16 cores co-stabilized by strong by σ- and π-bonded tert-butylethynide and CF3 COO(-) (together with methanol molecule in 2)...
October 12, 2016: Chemistry: a European Journal
Mirjam Cl Peek, Tibor Kovacs, Rose Baker, Hisham Hamed, Ash Kothari, Michael Douek
BACKGROUND: In early breast cancer, the optimal technique for sentinel lymph node biopsy (SLNB) is the combined technique (radioisotope and Patent Blue V) which achieves high identification rates. Despite this, many centres have decided to stop using blue dye due to blue-dye-related complications (tattoo, anaphylaxis). We evaluated the SLNB identification rate using the combined technique with and without Patent Blue V and the blue-dye-related complication rates. METHODS: Clinical and histological data were analysed on patients undergoing SLNB between March 2014 and April 2015...
2016: Ecancermedicalscience
Ning Wen, Siming Lu, Jinkoo Kim, Yujiao Qin, Yimei Huang, Bo Zhao, Chang Liu, Indrin J Chetty
PURPOSE: The purpose of this study is to evaluate the dosimetric uncertainty associated with Gafchromic™ (EBT3) films and establish a practical and efficient film dosimetry protocol for Stereotactic Radiosurgery (SRS) and Stereotactic Body Radiotherapy (SBRT). METHOD AND MATERIALS: EBT3 films were irradiated at each of seven different dose levels between 1 and 15 Gy with open fields and standard deviations of dose maps were calculated at each color channel for evaluation...
October 4, 2016: Radiation Oncology
David J T Campbell, Marcello Tonelli, Brenda Hemmelgarn, Chad Mitchell, Ross Tsuyuki, Noah Ivers, Tavis Campbell, Raj Pannu, Eric Verkerke, Scott Klarenbach, Kathryn King-Shier, Peter Faris, Derek Exner, Vikas Chaubey, Braden Manns
BACKGROUND: Chronic diseases result in significant morbidity and costs. Although medications and lifestyle changes are effective for improving outcomes in chronic diseases, many patients do not receive these treatments, in part because of financial barriers, patient and provider-level knowledge gaps, and low patient motivation. The Assessing outcomes of enhanced chronic disease care through patient education and a value-based formulary study (ACCESS) will determine the impact of two interventions: (1) a value-based formulary which eliminates copayment for high-value preventive medications; and (2) a comprehensive self-management support program aimed at promoting health behavior change and medication adherence, combined with relay of information on medication use to healthcare providers, on cardiovascular events and/or mortality in low-income seniors with elevated cardiovascular risk...
September 26, 2016: Implementation Science: IS
Ying-Jie Ning, Shan-Shan Wang, Qing-Jun Chen, Zhuo-Ren Ling, Shou-Nan Wang, Wen-Ping Wang, Guo-Qing Zhang, Meng-Juan Zhu
A novel laccase was isolated from fermentation broth of the mycorrhizal fungus Leucoagaricus naucinus LAC-04 by using a protocol that comprising ion-exchange chromatography steps on DEAE-cellulose, SP-Sepharose, and Q-Sepharose, and finally gel filtration by fast protein liquid chromatography on Superdex 75. The laccase (LNL) was purified with a purification fold of 21.19 and a recovery rate of 19.8%. It is a monomeric protein with a molecular mass of 56kDa. LNL lacks absorption around 600nm, which indicates that the purified laccase is a yellow laccases...
September 15, 2016: International Journal of Biological Macromolecules
Jessica Bruijel, Wisse P van der Meijden, Denise Bijlenga, Farangis Dorani, Joris E Coppens, Bart H W Te Lindert, J J Sandra Kooij, Eus J W Van Someren
Melanopsin-containing retinal ganglion cells play an important role in the non-image forming effects of light, through their direct projections on brain circuits involved in circadian rhythms, mood and alertness. Individual differences in the functionality of the melanopsin-signaling circuitry can be reliably quantified using the maximum post-illumination pupil response (PIPR) after blue light. Previous protocols for acquiring PIPR relied on the use of mydriatics to dilate the light-exposed eye. However, pharmacological pupil dilation is uncomfortable for the participants and requires ophthalmological expertise...
2016: Biology
Shelby F Thames, Joshua B White, Kevin L Ong
INTRODUCTION AND HYPOTHESIS: Polypropylene is a base polymer used in biomaterial applications, including sutures and mesh products, for the treatment of pelvic organ prolapse, stress urinary incontinence, and hernia repairs. Previous studies have dismissed the value of formulation additives employed in polypropylene, and the importance and necessity of an effective mesh explant cleaning protocol when characterizing explanted devices. However, both are critical to understanding the alleged degradation of polypropylene-based meshes...
September 6, 2016: International Urogynecology Journal
Jae-Hyeok Lee, Sun-Yong Baek, YoungKyu Song, Sujeong Lim, Hansol Lee, Minh Phuong Nguyen, Eun-Joo Kim, Gi Yeong Huh, Se Young Chun, HyungJoon Cho
High field magnetic resonance imaging (MRI)-based delineation of the substantia nigra (SN) and visualization of its inner cellular organization are promising methods for the evaluation of morphological changes associated with neurodegenerative diseases; however, corresponding MR contrasts must be matched and validated with quantitative histological information. Slices from two postmortem SN samples were imaged with a 7 Tesla (7T) MRI with T1 and T2* imaging protocols and then stained with Perl's Prussian blue, Kluver-Barrera, tyrosine hydroxylase, and calbindin immunohistochemistry in a serial manner...
2016: Scientific Reports
Fred Stephen Sarfo, Frank Treiber, Carolyn Jenkins, Sachin Patel, Mulugeta Gebregziabher, Arti Singh, Osei Sarfo-Kantanka, Raelle Saulson, Lambert Appiah, Eunice Oparebea, Bruce Ovbiagele
BACKGROUND: Hypertension is the premier modifiable risk factor for recurrent stroke. In sub-Saharan Africa (SSA) where the stroke burden is escalating, little is known about the role of behavioral interventions in enhancing blood pressure (BP) control after stroke. Our objective is to test whether an m-Health technology-enabled, nurse-led, multilevel integrated approach is effective in improving BP control among Ghanaian stroke patients within 1 month of symptom onset compared with standard of care...
2016: Trials
Donata Magrini, Susanna Bracci, Emma Cantisani, Claudia Conti, Antonio Rava, Antonio Sansonetti, Will Shank, MariaPerla Colombini
Samples from Keith Haring's wall painting of the Necker Children Hospital in Paris were studied by a multi-analytical protocol. X-ray fluorescence (XRF), powder X-ray diffraction (XRDP), Electron microscope (SEM-EDS), Infrared and Raman spectroscopy (μ-FT-IR and μ-Raman) measurements were performed in order to characterize the materials and to identify the art technique used to produce this contemporary work. Materials from the mural suffered from severe detachments of materials and several fragments were found on the ground beneath...
August 18, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
M M Torvund, T S Ma, V P Connaughton, F Ono, R F Nelson
Strata within the inner plexiform layer (IPL) of vertebrate retinas are suspected to be distinct signaling regions. Functions performed within adult zebrafish IPL strata were examined through microelectrode recording and staining of stratified amacrine types. The stimulus protocol and analysis discriminated the pattern of input from red, green, blue, and UV cones as well as the light-response waveforms in this tetrachromatic species. A total of 36 cells were analyzed. Transient depolarizing waveforms at ON and OFF originated with bistratified amacrine types, whose dendritic planes branched either in IPL sublaminas a & b, or only within sublamina a...
August 29, 2016: Journal of Comparative Neurology
Tuomas K Niemi, Melissa A Marchesan, Adam Lloyd, Robert J Seltzer
INTRODUCTION: The aim of this study was to compare the effectiveness of TRUShape (TS) instruments with ProFile Vortex Blue (VB) instruments for the removal of obturation materials during retreatment of single-canal mandibular premolars performed through 2 access outlines. METHODS: Initial root canal treatment was completed through a contracted endodontic cavity (CEC) design. Canals were instrumented to an F2 ProTaper instrument, obturated with warm lateral condensation of gutta-percha with AH Plus sealer, and allowed to set for 30 days at 37°C and 100% humidity...
October 2016: Journal of Endodontics
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