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https://www.readbyqxmd.com/read/29609271/-killing-effect-of-robo1-targeted-chimeric-antigen-receptor-modified-nk92-cells-against-glioma-and-neuroblastoma-cells
#1
Y Qu, J Z Bi
Objective: To study the cytotoxicity of Robo1-CAR-NK92 cells against U87-MG and SH-SY5Y cells, to explore the effects of IL-15, IL-21 and dexamethasone on the proliferation, survival and cytotoxicity of Robo1-CAR-NK92 cells and to optimize the culture protocol. Methods: Robo1-CAR-NK92 cells were constructed by lentivirus transfection.The Robo1 car positive cells were sorted, expanded and detected by flow cytometry.The levels of Robo1 expression in SH-SY5Y and U87-MG cells were examined by flow cytometry.The cytotoxicity of Robo1-CAR-NK92 or NK92 cells against target cells was tested by CCK-8 and live cell imaging...
March 20, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29606555/current-methodology-of-mtt-assay-in-bacteria-a-review
#2
REVIEW
Ewa Grela, Joanna Kozłowska, Agnieszka Grabowiecka
The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay is a popular tool in estimating the metabolic activity of living cells. The test is based on enzymatic reduction of the lightly colored tetrazolium salt to its formazan of intense purple-blue color, which can be quantified spectrophotometrically. Under properly optimized conditions the obtained absorbance value is directly proportional to the number of living cells. Originally, the MTT assay was devised for use in eukaryotic cells lines and later applied for bacteria and fungi...
March 29, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29596843/viability-assessment-of-primary-growth-oocytes-following-ovarian-tissue-vitrification-of-neotropical-teleost-pacu-piaractus-mesopotamicus
#3
Lis S Marques, Ana A Fossati, Martinha S Leal, Rômulo B Rodrigues, Robie A Bombardelli, Danilo P Streit
Vitrification of ovarian tissue containing immature oocytes provides an important tool for protecting the endangered species and genetic diversity in aquatic species. Therefore, the main objective was to assess primary growth (PG) oocytes viability following ovarian tissue vitrification using histological analysis, two staining protocols (trypan blue or fluorescein diacetate combined with propidium iodide) and mitochondrial activity assay (MTT assay). In addition, oocyte histomorphometry was performed to evaluate the morphometric parameters after vitrification and the relationship with the occurrence of damage (nucleus and/or membrane) in PG oocytes...
March 26, 2018: Cryobiology
https://www.readbyqxmd.com/read/29596478/association-of-socioeconomic-status-with-hearing-loss-in-chinese-working-aged-adults-a-population-based-study
#4
Ping He, Yanan Luo, Xiangyang Hu, Rui Gong, Xu Wen, Xiaoying Zheng
Hearing loss is the most common sensory impairment, but limited studies focused on the association of socioeconomic status (SES) with hearing loss among adults of working age. This paper aimed to fill this gap among Chinese adults. We obtained data from Ear and Hearing Disorder Survey conducted in four provinces of China in 2014-2015. The survey was based on WHO Ear and Hearing Disorders Survey Protocol and 25,860 adults aged 25 to 59 years were selected in this study. Trained local examiners performed pure tone audiometry to screen people with hearing loss, and those who were screened positively for hearing loss were referred to audiologists to make final diagnosis...
2018: PloS One
https://www.readbyqxmd.com/read/29593685/the-use-of-a-chimeric-rhodopsin-vector-for-the-detection-of-new-proteorhodopsins-based-on-color
#5
Alina Pushkarev, Gur Hevroni, Sheila Roitman, Jin-Gon Shim, Ahreum Choi, Kwang-Hwan Jung, Oded Béjà
Student microbial ecology laboratory courses are often conducted as condensed courses in which theory and wet lab work are combined in a very intensive short time period. In last decades, the study of marine microbial ecology is increasingly reliant on molecular-based methods, and as a result many of the research projects conducted in such courses require sequencing that is often not available on site and may take more time than a typical course allows. In this work, we describe a protocol combining molecular and functional methods for analyzing proteorhodopsins (PRs), with visible results in only 4-5 days that do not rely on sequencing...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29589176/photodynamic-inactivation-in-the-expression-of-the-candida-albicans-genes-als3-hwp1-bcr1-tec1-cph1-and-efg1-in-biofilms
#6
Fernanda Freire, Patrícia Pimentel de Barros, Cristiane Aparecida Pereira, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
The objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time...
March 27, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29582671/a-standardized-method-of-applying-toluidine-blue-metachromatic-staining-for-assessment-of-chondrogenesis
#7
Natasja Leth Bergholt, Helle Lysdahl, Martin Lind, Casper Bindzus Foldager
Objectives Staining with toluidine blue is a well-established procedure for the histological assessment of cartilaginous- and chondrogenic-differentiated tissues. Being a cationic dye, toluidine blue staining visualizes proteoglycans in a tissue because of its high affinity for the sulfate groups in proteoglycans. It is generally accepted that metachromatic staining with toluidine blue represents cartilaginous matrix and that the degree of positive staining corresponds with the amount of proteoglycans. Design Articular cartilage and pellets of chondrocytes or bone marrow stromal cells were analyzed with a standardized staining procedure for toluidine blue...
March 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29579410/dose-and-administration-protocol-for-fsh-used-for-ovarian-stimulation-affect-gene-expression-in-sheep-cumulus-oocyte-complexes
#8
Gláucia M Bragança, Ribrio Ivan T P Batista, Joanna Maria G Souza-Fabjan, Vivian A P Alfradique, Eduardo K N Arashiro, Isabel O Cosentino, Pedro Henrique N Pinto, Luiz Sérgio A Camargo, Jeferson F da Fonseca, Felipe Z Brandão
The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus-oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P>0...
March 27, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29578414/a-mm-sized-wireless-implantable-device-for-electrical-stimulation-of-peripheral-nerves
#9
Jayant Charthad, Ting Chia Chang, Zhaokai Liu, Ahmed Sawaby, Marcus J Weber, Sam Baker, Felicity Gore, Stephen A Felt, Amin Arbabian
A wireless electrical stimulation implant for peripheral nerves, achieving >10× improvement over state of the art in the depth/volume figure of merit, is presented. The fully integrated implant measures just 2 mm × 3 mm × 6.5 mm (39 mm3 , 78 mg), and operates at a large depth of 10.5 cm in a tissue phantom. The implant is powered using ultrasound and includes a miniaturized piezoelectric receiver (piezo), an IC designed in 180 nm HV BCD process, an off-chip energy storage capacitor, and platinum stimulation electrodes...
April 2018: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/29569874/comparison-of-different-histological-protocols-for-the-preservation-and-quantification-of-the-intestinal-mucus-layer-in-pigs
#10
Ilen Röhe, Friedrich Joseph Hüttner, Johanna Plendl, Barbara Drewes, Jürgen Zentek
The histological characterization of the intestinal mucus layer is important for many scientific experiments investigating the interaction between intestinal microbiota, mucosal immune response and intestinal mucus production. The aim of this study was to examine and compare different fixation protocols for displaying and quantifying the intestinal mucus layer in piglets and to test which histomorphological parameters may correlate with the determined mucus layer thickness. Jejunal and colonal tissue samples of weaned piglets (n=10) were either frozen in liquid nitrogen or chemically fixed using methacarn solution...
February 5, 2018: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29564768/whole-mount-and-section-in-situ-hybridization-in-mouse-embryos-for-detecting-mrna-expression-and-localization
#11
Kazuko Koshiba-Takeuchi
In situ hybridization is defined as one of the most useful and powerful methods to know where genes (e.g., mRNA, ncRNA) of interest are expressed in tissues. Expression of mRNA can be detected as blue or dark purple signals though hybridization, immunoreaction and coloring steps. Genome-wide approaches in various model animals have been conducted thoroughly, and have led to new research areas aimed at uncovering novel gene functions in cell differentiation and development. To elucidate gene function, spatiotemporal gene expression analysis is very important...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29561895/untangling-the-r2-contrast-in-multiple-sclerosis-a-combined-mri-histology-study-at-7-0-tesla
#12
Francesca Bagnato, Simon Hametner, Emma Boyd, Verena Endmayr, Yaping Shi, Vasiliki Ikonomidou, Guanhua Chen, Siddharama Pawate, Hans Lassmann, Seth Smith, E Brian Welch
T2*-weighted multi-echo gradient-echo magnetic resonance imaging and its reciprocal R2* are used in brain imaging due to their sensitivity to iron content. In patients with multiple sclerosis who display pathological alterations in iron and myelin contents, the use of R2* may offer a unique way to untangle mechanisms of disease. Coronal slices from 8 brains of deceased multiple sclerosis patients were imaged using a whole-body 7.0 Tesla MRI scanner. The scanning protocol included three-dimensional (3D) T2*-w multi-echo gradient-echo and 2D T2-w turbo spin echo (TSE) sequences...
2018: PloS One
https://www.readbyqxmd.com/read/29558563/rediscovering-ducos-du-hauron-s-color-photography-through-a-review-of-his-three-color-printing-processes-and-synchrotron-microanalysis-of-his-prints
#13
Marine Cotte, Tiphaine Fabris, Juliette Langlois, Ludovic Bellot-Gurlet, Françoise Ploye, Natalie Coural, Clotilde Boust, Jean-Paul Gandolfo, Thomas Galifot, Jean Susini
Louis Ducos du Huron (1837-1920) dedicated his entire life to the elaboration of physical-chemical processes for color photography. This study aimed at highlighting his unique contribution to three-color printing through 1) an in-depth review of the many protocols he published and 2) the synchrotron-based IR and X-ray microanalysis of fragments sampled in three artworks. Ducos du Hauron's method relied on the preparation and assembly of three monochromes (red, blue, yellow). This study brings to light complex multistep recipes based on photochemistry (carbon print), organic, and inorganic chemistry...
March 20, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29550738/a-novel-modularized-optical-sensor-for-ph-monitoring-in-biological-matrixes
#14
Xun Liu, Shang-Qing Zhang, Xing Wei, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A novel core-shell structure optical pH sensor is developed with upconversion nanoparticles (UCNPs) serving as the core and silica as the shell, followed by grafting bovineserumalbumin (BSA) as another shell via glutaraldehyde cross-linking. The obtained core-shell-shell structure is shortly termed as UCNPs@SiO2 @BSA, and its surface provides a platform for loading various pH sensitive dyes, which are alike "modules" to make it feasible for measuring pHs within different pH ranges by simply regulating the type of dyes...
March 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29545993/combined-staining-techniques-for-demonstration-of-staphylococcus-aureus-biofilm-in-routine-histopathology
#15
Louise Kruse Jensen, Nicole Lind Henriksen, Thomas Bjarnsholt, Kasper Nørskov Kragh, Henrik Elvang Jensen
Aim: Visualization of Staphylococcus aureus biofilm using histochemical staining and combined histochemistry (HC) and immunohistochemistry (IHC). Methods: The ability of S. aureus S54F9 to form biofilm was tested in vitro . Hereafter, infected bone tissue was collected from two different porcine models of osteomyelitis inoculated with S. aureus strain S54F9. The infection time was five and fifteen days, respectively. Twenty-five different histochemical staining protocols were tested in order to find the stains that could identify extracellular biofilm matrix...
2018: Journal of Bone and Joint Infection
https://www.readbyqxmd.com/read/29535635/die-rna-a-reproducible-strategy-for-the-digestion-of-normal-and-injured-pancreas-isolation-of-pancreatic-cells-from-genetically-engineered-mouse-models-and-extraction-of-high-quality-rna
#16
Mohamad Assi, Nicolas Dauguet, Patrick Jacquemin
The isolation of ribonucleic acid (RNA) suitable for gene expression studies is challenging in the pancreas, due to its high ribonuclease activity. This is even more complicated during pancreatitis, a condition associated with inflammation and fibrosis. Our aim was to implement a time-effective and reproducible protocol to isolate high quality RNA from specific pancreatic cell subtypes, in normal and inflammatory conditions. We used two genetically engineered mouse models (GEMM), Ela-CreER/YFP and Sox9-CreER/YFP, to isolate acinar and ductal cells, respectively...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29528110/gold-nanoparticles-crosslinking-by-peptides-and-amino-acids-a-tool-for-the-colorimetric-identification-of-amino-acids
#17
Álvaro Martínez, Paolo Scrimin
Gold nanoparticles are known to aggregate in the presence of proper multifunctional compounds. For those larger than 3 nm, the process can be followed with the naked eye because the surface plasmon absorption band of the particles shifts to longer wavelengths and the solution color changes from red to blue. To exploit this property, we have used amino acids and peptides as crosslinking agents in ethanol at low µM concentrations. In the case of amino acids, we report a straightforward protocol for their colorimetric identification...
March 12, 2018: Biopolymers
https://www.readbyqxmd.com/read/29527416/immobilized-tio-2-on-glass-spheres-applied-to-heterogeneous-photocatalysis-photoactivity-leaching-and-regeneration-process
#18
Deivisson Lopes Cunha, Alexei Kuznetsov, Carlos Alberto Achete, Antonio Eduardo da Hora Machado, Marcia Marques
Heterogeneous photocatalysis using titanium dioxide as catalyst is an attractive advanced oxidation process due to its high chemical stability, good performance and low cost. When immobilized in a supporting material, additional benefits are achieved in the treatment. The purpose of this study was to develop a simple protocol for impregnation of TiO2 -P25 on borosilicate glass spheres and evaluate its efficiency in the photocatalytic degradation using an oxidizable substrate (methylene blue), in a Compound Parabolic Concentrator (CPC) reactor...
2018: PeerJ
https://www.readbyqxmd.com/read/29523862/antimicrobial-photodynamic-therapy-enhanced-by-the-peptide-aurein-1-2
#19
Laura Marise de Freitas, Esteban Nicolás Lorenzón, Norival Alves Santos-Filho, Lucas Henrique de Paula Zago, Marciana Pierina Uliana, Kleber Thiago de Oliveira, Eduardo Maffud Cilli, Carla Raquel Fontana
In the past few years, the World Health Organization has been warning that the post-antibiotic era is an increasingly real threat. The rising and disseminated resistance to antibiotics made mandatory the search for new drugs and/or alternative therapies that are able to eliminate resistant microorganisms and impair the development of new forms of resistance. In this context, antimicrobial photodynamic therapy (aPDT) and helical cationic antimicrobial peptides (AMP) are highlighted for the treatment of localized infections...
March 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29508105/decellularized-amnion-scaffold-with-activated-prp-a-new-paradigm-dressing-material-for-burn-wound-healing
#20
Jeevitaa Kshersagar, Ravi Kshirsagar, Shashikant Desai, Raghvendra Bohara, Meghnad Joshi
Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD)...
March 5, 2018: Cell and Tissue Banking
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