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https://www.readbyqxmd.com/read/28632841/green-light-autofluorescence-versus-combined-blue-light-autofluorescence-and-near-infrared-reflectance-imaging-in-geographic-atrophy-secondary-to-age-related-macular-degeneration
#1
Maximilian Pfau, Lukas Goerdt, Steffen Schmitz-Valckenberg, Matthias M Mauschitz, Divyansh K Mishra, Frank G Holz, Moritz Lindner, Monika Fleckenstein
Purpose: To compare the intermodality and interreader agreement for geographic atrophy (GA) lesion size quantification in green-light fundus autofluorescence (GAF; excitation = 518 nm) versus combined blue-light fundus autofluorescence (BAF; excitation = 488 nm) and near-infrared reflectance (NIR; 820 nm) -based grading. Methods: Confocal scanning laser ophthalmoscopy (cSLO) GAF, BAF, and NIR images of 40 eyes from 29 patients (mean age 79.7 years) with GA secondary to AMD were recorded according to a standardized protocol...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28624146/the-impact-of-the-blue-protocol-ultrasonography-on-the-time-taken-to-treat-acute-respiratory-distress-in-the-emergency-department
#2
Javad Seyedhosseini, Golnaz Bashizadeh-Fakhar, Shirani Farzaneh, Mehdi Momeni, Ehsan Karimialavijeh
No abstract text is available yet for this article.
June 6, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28622352/volunteer-trials-of-a-novel-improvised-dry-decontamination-protocol-for-use-during-mass-casualty-incidents-as-part-of-the-uk-s-initial-operational-response-ior
#3
Richard Amlôt, Holly Carter, Lorna Riddle, Joanne Larner, Robert P Chilcott
Previous studies have demonstrated that rapid evacuation, disrobing and emergency decontamination can enhance the ability of emergency services and acute hospitals to effectively manage chemically-contaminated casualties. The purpose of this human volunteer study was to further optimise such an "Initial Operational Response" by (1) identifying an appropriate method for performing improvised skin decontamination and (2) providing guidance for use by first responders and casualties. The study was performed using two readily available, absorbent materials (paper towels and incontinence pads)...
2017: PloS One
https://www.readbyqxmd.com/read/28620429/novel-approaches-to-ultrasonography-of-the-lung-and-pleural-space-where-are-we-now
#4
REVIEW
Daniel Lichtenstein
This review article is an update of what should be known for practicing basic lung ultrasound in the critically ill (LUCI) and is also of interest for less critical disciplines (e.g. pulmonology). It pinpoints on the necessity of a professional machine (not necessarily a sophisticated one) and probe. It lists the 10 main signs of LUCI and some of the main protocols made possible using LUCI: the BLUE protocol for a respiratory failure, the FALLS protocol for a circulatory failure, the SESAME protocol for a cardiac arrest and the investigation of a ventilated acute respiratory distress syndrome patient, etc...
June 2017: Breathe
https://www.readbyqxmd.com/read/28619615/evaluation-of-antimicrobial-activity-of-association-of-chlorhexidine-to-photosensitizer-used-in-photodynamic-therapy-in-root-canals-infected-by-enterococcus-faecalis
#5
Matheus Albino Souza, Guilherme Lima, Bianca Pazinatto, Karolina Frick Bischoff, Huriel Scartazzini Palhano, Doglas Cecchin
INTRODUCTION: The aim of the present study was to evaluate, in vitro, the influence of the addition of chlorhexidine to photosensitiser in the antimicrobial activity of photodynamic therapy in root canals infected by Enterococcus faecalis. METHODS: the root canals of 50 single-rooted human extracted teeth were enlarged up to a file F3 of Pro-Taper system, autoclaved, inoculated with Enterococcus faecalis and incubated for 14 days. The samples were divided into five groups (n=10) according to the protocol of decontamination: G1 (control group) - no procedure was performed; G2-photosensitiser (0...
June 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28615276/bluehealth-a-study-programme-protocol-for-mapping-and-quantifying-the-potential-benefits-to-public-health-and-well-being-from-europe-s-blue-spaces
#6
James Grellier, Mathew P White, Maria Albin, Simon Bell, Lewis R Elliott, Mireia Gascón, Silvio Gualdi, Laura Mancini, Mark J Nieuwenhuijsen, Denis A Sarigiannis, Matilda van den Bosch, Tanja Wolf, Susanne Wuijts, Lora E Fleming
INTRODUCTION: Proximity and access to water have long been central to human culture and accordingly deliver countless societal benefits. Over 200 million people live on Europe's coastline, and aquatic environments are the top recreational destination in the region. In terms of public health, interactions with 'blue space' (eg, coasts, rivers, lakes) are often considered solely in terms of risk (eg, drowning, microbial pollution). Exposure to blue space can, however, promote health and well-being and prevent disease, although underlying mechanisms are poorly understood...
June 14, 2017: BMJ Open
https://www.readbyqxmd.com/read/28608220/calpain-zymography-general-methodology-and-protocol
#7
Kevin K W Wang
Casein zymography has become one of the gold standard assays for monitoring mammalian calcium-activated proteases (calpains) in purified enzyme, cell, or tissue samples. This calpain zymography method takes advantages of (1) casein is an excellent substrate for major isoforms of calpains (Calpain-1, 2 and 3), (2) the embedded casein is digested into small peptides where the calpain bands are located, thus creating a clear zone upon Commassie blue gel staining, and (3) the calpain isoforms have different gel mobility under native gel conditions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28608215/zymography-in-multiwells-for-quality-assessment-of-proteinases
#8
Ambili Mechoor, Madathiparambil G Madanan
Zymography is a well-standardized protocol for the qualitative assessment and analysis of proteinases under specified conditions. However, analysis of a large number of samples simultaneously becomes a challenge when the zymography is carried out by the usual protocol of electrophoresis. This can be overcome by assaying the matrix-degrading proteinases in substrate-impregnated gels in multiwells. Enzymes are copolymerized with 300 mL of 10% acrylamide impregnated with gelatin substrate and incubated for 16 h...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28608200/mmp-activity-detection-in-zymograms
#9
Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska, Gergő Szűcs, Péter Ferdinandy
Matrix metalloproteinases (MMP) belong to a distinguished class of zinc-dependent endopeptidases. Zymography is a semi-quantitative tool for determining the activity of different MMP isoenzymes in a variety of biological samples. In substrate gel zymography, protein samples of different origin (tissue, cell lysates, plasma/serum, perfusates, other liquids) are separated in sodium dodecyl sulfate (SDS) polyacrylamide gels containing copolymerized substrate (gelatin, casein, elastin, etc.), and after incubation-enabling substrate cleavage by MMPs, MMP activities are detected after the gel staining as transparent bands against a dark-blue background...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28540349/dataset-of-protein-species-from-human-liver
#10
Stanislav Naryzhny, Maria Maynskova, Victor Zgoda, Alexander Archakov
This article contains data related to the research article entitled "Zipf׳s law in proteomics" (Naryzhny et al., 2017) [1]. The protein composition in the human liver or hepatocarcinoma (HepG2) cells extracts was estimated using a filter-aided sample preparation (FASP) protocol. The protein species/proteoform composition in the human liver was determined by two-dimensional electrophoresis (2-DE) followed by Electrospray Ionization Liquid Chromatography-Tandem Mass Spectrometry (ESI LC-MS/MS). In the case of two-dimensional electrophoresis (2-DE), the gel was stained with Coomassie Brilliant Blue R350, and image analysis was performed with ImageMaster 2D Platinum software (GE Healthcare)...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28526089/adipose-derived-mesenchymal-stem-cells-from-liposuction-and-resected-fat-are-feasible-sources-for-regenerative-medicine
#11
Sandra Schneider, Marina Unger, Martijn van Griensven, Elizabeth R Balmayor
BACKGROUND: The use of mesenchymal stem cells (MSCs) in research and in regenerative medicine has progressed. Bone marrow as a source has drawbacks because of subsequent morbidities. An easily accessible and valuable source is adipose tissue. This type of tissue contains a high number of MSCs, and obtaining higher quantities of tissue is more feasible. Fat tissue can be harvested using different methods such as liposuction and resection. First, a detailed isolation protocol with complete characterization is described...
May 19, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28518109/assessment-of-neuronal-viability-using-fluorescein-diacetate-propidium-iodide-double-staining-in-cerebellar-granule-neuron-culture
#12
Lin Jiajia, Mak Shinghung, Zheng Jiacheng, Wang Jialing, Xu Dilin, Hu Shengquan, Zhang Zaijun, Wang Qinwen, Han Yifan, Cui Wei
Primary cultured Cerebellar Granule Neurons (CGNs) have been widely used as an in vitro model in neuroscience and neuropharmacology research. However, the co-existence of glial cells and neurons in CGN culture might lead to biases in the accurate assessment of neuronal viability. Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining has been used to measure cell viability by simultaneously evaluating the viable and dead cells. We used FDA-PI double staining to improve the sensitivities of the colorimetric assays and to evaluate neuronal viability in CGNs...
May 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518105/murine-lymphocyte-labeling-by-64cu-antibody-receptor-targeting-for-in-vivo-cell-trafficking-by-pet-ct
#13
Sabrina H L Hoffmann, Andreas Maurer, Dorothea I Reck, Gerald Reischl, Bernd J Pichler, Manfred Kneilling, Christoph M Griessinger
This protocol illustrates the production of (64)Cu and the chelator conjugation/radiolabeling of a monoclonal antibody (mAb) followed by murine lymphocyte cell culture and (64)Cu-antibody receptor targeting of the cells. In vitro evaluation of the radiolabel and non-invasive in vivo cell tracking in an animal model of an airway delayed-type hypersensitivity reaction (DTHR) by PET/CT are described. In detail, the conjugation of a mAb with the chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) is shown...
April 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518072/in-vivo-investigation-of-antimicrobial-blue-light-therapy-for-multidrug-resistant-acinetobacter-baumannii-burn-infections-using-bioluminescence-imaging
#14
Yucheng Wang, Olivia D Harrington, Ying Wang, Clinton K Murray, Michael R Hamblin, Tianhong Dai
Burn infections continue to be an important cause of morbidity and mortality. The increasing emergence of multidrug-resistant (MDR) bacteria has led to the frequent failure of traditional antibiotic treatments. Alternative therapeutics are urgently needed to tackle MDR bacteria. An innovative non-antibiotic approach, antimicrobial blue light (aBL), has shown promising effectiveness against MDR infections. The mechanism of action of aBL is not yet well understood. It is commonly hypothesized that naturally occurring endogenous photosensitizing chromophores in bacteria (e...
April 28, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516115/a-modified-chromogenic-assay-for-determination-of-the-ratio-of-free-intracellular-nad-nadh-in-streptococcus-mutans
#15
Jonathon L Baker, Roberta C Faustoferri, Robert G Quivey
Nicotinamide adenine dinucleotide is a coenzyme present in all kingdoms of life and exists in two forms: oxidized (NAD(+)) and reduced (NADH). NAD(H) is involved in a multitude of essential metabolic redox reactions, providing oxidizing or reducing equivalents. The ratio of free intracellular NAD(+)/NADH is fundamentally important in the maintenance of cellular redox homeostasis (Ying, 2008). Various chromogenic cycling assays have been used to determine the ratio of NAD(+)/NADH in both bacterial and mammalian cells for more than forty years (Bernofsky and Swan, 1973; Nisselbaum and Green, 1969)...
August 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28516113/pit-assay-to-measure-the-bone-resorptive-activity-of-bone-marrow-derived-osteoclasts
#16
Alexander Vesprey, Wentian Yang
Although it is possible to use a tartrate-resistant acid phosphatase (TRAP) stain to assist in identifying osteoclasts, a separate method is needed to determine the bone resorption activity of osteoclasts. Since osteoclasts leave "pits" after bone matrix resorption (Charles et al., 2014), it is possible to stain pits as a method of measuring osteoclast bone resorption activity. The pit assay protocol enables researchers to stain bony slices that were co-cultured with osteoclasts with toluidine blue in order to allow the visualization, capture, and analysis of osteoclast resorptive activity based on the number, size and depth of pits (Zhou et al...
June 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28508640/construction-of-polysubstituted-1-2-dihydrocyclobuta-b-naphthalenes-and-1-2-dihydrocyclobuta-b-anthracenes-with-photoluminescence
#17
Dianpeng Chen, Lianpeng Zhang, Jinzhong Yao, Hongwei Zhou
A facile protocol for the synthesis of polysubstituted 1,2-dihydrocyclobuta[b]naphthalenes and 1,2-dihydrocyclobuta[b]anthracenes was developed via a sequence of base-promoted 1,5-H shift, intramolecular [2 + 2] cycloaddition, and aromatization. The synthesized 1,2-dihydrocyclobuta[b]anthracenes exhibited bright blue emissions in solution and strong yellow emissions in solid, which made them possible candidates for optoelectronic conjugated materials.
May 24, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28508364/a-novel-2-de-based-proteomic-analysis-to-identify-multiple-substrates-for-specific-protease-in-neuronal-cells
#18
Chiho Kim, Young J Oh
Proteolysis is a process where proteins are broken down into smaller polypeptides or amino acids, comprising one of the important posttranslational modifications of proteins. Since this process is exquisitely achieved by specialized enzymes called proteases under physiological conditions, abnormal protease activity and dysregulation of their substrate proteins are closely associated with a progression of several neurodegenerative diseases including Alzheimer disease, Parkinson disease, stroke, and spinal cord injury...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28508242/preclinical-study-of-a-cost-effective-photodynamic-therapy-protocol-for-treating-oral-candidoses
#19
Nathalia Ramos da Silva, Daniela Garcia Ribeiro, João Paulo Mardegan Issa, Karla Bonfá, Michelli Sobreiro Menezes, Viviane de Cássia Oliveira, Raphael Freitas de Souza
Photodynamic therapy (PDT) is a promising treatment for oral candidoses. Its use as an alternative to antifungals prevents several adverse effects, including microbial resistance. However, most PDT protocols do not employ devices and consumables commonly available in dental practice, thus influencing treatment affordability. This study aimed to determine the efficacy of a PDT method based on light curing units' blue LEDs combined to a plaque-disclosing composition (5% erythrosine) against C. albicans in culture and in a murine model of oral candidosis...
May 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28506653/inhibition-of-denture-plaque-deposition-on-complete-dentures-by-2-methacryloyloxyethyl-phosphorylcholine-polymer-coating-a-clinical-study
#20
Kenji Ikeya, Fuminori Iwasa, Yuuki Inoue, Miya Fukunishi, Nana Takahashi, Kazuhiko Ishihara, Kazuyoshi Baba
STATEMENT OF PROBLEM: Denture plaque-associated infections are regarded as a source of serious dental and medical complications in the elderly population. Methods of managing this problem are needed. PURPOSE: The purpose of this clinical study was to evaluate the effects of treatment with a 2-methacryloyloxyethyl phosphorylcholine polymer, PMBPAz, on plaque deposition in complete dentures. MATERIAL AND METHODS: The study protocol was approved by the Ethics Committee of Showa University (#2013-013)...
May 12, 2017: Journal of Prosthetic Dentistry
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