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https://www.readbyqxmd.com/read/28448011/turbidimetry-on-human-washed-platelets-the-effect-of-the-pannexin1-inhibitor-brilliant-blue-fcf-on-collagen-induced-aggregation
#1
Filippo Molica, Séverine Nolli, Pierre Fontana, Brenda Renata Kwak
Turbidimetry is a laboratory technique that is applied to measure the aggregation of platelets suspended in either plasma (platelet-rich plasma, PRP) or in buffer (washed platelets), by the use of one or a combination of agonists. The use of washed platelets separated from their plasma environment and in the absence of anticoagulants allows for studying intrinsic platelet properties. Among the large panel of agonists, arachidonic acid (AA), adenosine di-phosphate (ADP), thrombin and collagen are the most frequently used...
April 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28433858/reduced-graphene-oxide-chitosan-aptamer-interface-as-new-platform-for-ultrasensitive-detection-of-human-epidermal-growth-factor-receptor-2
#2
Arezoo Tabasi, Abdollah Noorbakhsh, Ensiyeh Sharifi
The present work describes an ultrasensitive electrochemical aptamer-based assay for detection of human epidermal growth factor receptor 2 protein (HER2) cancer biomarker as a model analyte. Results show that the reduced graphene oxide-chitosan (rGO-Chit) film as a suitable electrode material possesses great favorable properties including high homogeneity, good stability, large surface area and high fraction of amine groups as aptamer binding sites. Various steps of aptasensor fabrication were characterized using microscopic, energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques...
April 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28427409/efficacies-of-dha-ppq-and-as-sp-in-patients-with-uncomplicated-plasmodium-falciparum-malaria-in-an-area-of-an-unstable-seasonal-transmission-in-sudan
#3
Abdelrahim O Mohamed, Muzamil M Abdel Hamid, Omer S Mohamed, Nuha S Elkando, Abdelmaroof Suliman, Mariam A Adam, Fahad Awad Ali Elnour, Elfatih M Malik
BACKGROUND: Artemisinin-based combination therapy (ACT), together with other control measures, have reduced the burden of falciparum malaria in sub-Saharan countries, including Sudan. Sudan adopted ACT in 2004 with a remarkable reduction in mortality due to falciparum malaria. However, emergence of resistance to the first-line treatment artesunate and sulfadoxine/pyrimethamine (AS/SP) has created new challenges to the control of malaria in Sudan. A search for an alternative drug of choice for treating uncomplicated malaria has become inevitable...
April 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28426570/susceptibility-of-trichopyton-mentagrophytes-to-visible-light-wavelengths
#4
J Stephen Guffey, William Payne, Ben Buchanan, Jessie Daugherty, Logan Meurer, Patricia Hensley
OBJECTIVE: To determine whether a blue light (405 nm) could inhibit the growth of Trichopyton mentagrophytes without using a photosensitizing material as part of the treatment protocol. DESIGN: Basic physiologic randomized trial using laboratory specimens (T mentagrophytes). INTERVENTIONS/METHODS: Plated on a growth medium, T mentagrophytes were exposed to 3 to 5 administrations of blue light at 20 J/cm over 28 hours. Following 7 days of incubation, colony-forming units were counted and compared with nonirradiated controls...
May 2017: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/28415479/natural-polymeric-microspheres-for-modulated-drug-delivery
#5
Costantino Del Gaudio, Valentina Crognale, Gianpaolo Serino, Pierluca Galloni, Alberto Audenino, Domenico Ribatti, Umberto Morbiducci
Microspheres can be regarded as a suitable platform for the development of ad hoc drug delivery systems, since the targeted release of a therapeutic agent can effectively contribute to support and improve a pharmacological protocol. However, several crucial factors related to the selection of materials, drugs and fabrication techniques should be critically analyzed in order to enhance the expected performance. Dealing with highly compatible materials, e.g. naturally-derived polymers and "green" reagents, can be a valid approach...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28409463/blue-native-page-applications-to-study-peroxisome-biogenesis
#6
Kanji Okumoto, Shigehiko Tamura, Yukio Fujiki
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is one of the useful methods to isolate protein complexes including membrane proteins under native conditions. In BN-PAGE, Coomassie Brilliant Blue G-250 binds to proteins and provides a negative charge for the electrophoretic separation without denaturing at neutral pH, allowing the analysis of molecular mass, oligomeric state, and composition of native protein complexes. BN-PAGE is widely applied to the characterization of soluble protein complexes as well as isolation of membrane protein complexes from biological membranes such as the complexes I-V of the mitochondrial respiratory chain and subcomplexes of the mitochondrial protein import machinery...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28406507/achieving-yellow-emission-by-varying-the-donor-acceptor-units-in-rod-shaped-fluorenyl-alkynyl-based-%C3%AF-conjugated-oligomers-and-their-binuclear-gold-i-alkynyl-complexes
#7
Sk Najmul Islam, Amit Sil, Sanjib K Patra
Fluorenyl-alkynyl based π-conjugated rod-shaped oligomers bearing different central aromatic moieties and functionalizable di-alkynyl termini, such as H-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-H (OH1), H-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-Btz-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-H (OH2) and H-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-Btd-[triple bond, length as m-dash]-Fl-[triple bond, length as m-dash]-H (OH3) where Fl = 9,9-dioctylfluorene, Btz = N-hexylbenzotriazole, and Btd = benzothiadiazole, were successfully synthesized by a Pd(0) catalyzed Stille coupling protocol...
April 13, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28405594/screening-for-novel-endogenous-inflammatory-stimuli-using-the-secreted-embryonic-alkaline-phosphatase-nf-%C3%AE%C2%BAb-reporter-assay
#8
Lorena Zuliani-Alvarez, Anna M Piccinini, Kim S Midwood
An immune response can be activated by pathogenic stimuli, as well as endogenous danger signals, triggering the activation of pattern recognition receptors and initiating signalling cascades that lead to inflammation. This method uses THP1-Blue™ cells, a human monocytic cell line which contains an embryonic alkaline phosphatase reporter gene allowing the detection of NF-κB-induced transcriptional activation. We validated this protocol by assessing NF-κB activation after stimulation of toll-like receptor 4 (TLR4) by two different agonists: lipopolysaccharide (LPS), derived from the cell wall of Gram negative bacteria, and tenascin-C, an extracellular matrix protein whose expression is induced upon tissue injury...
April 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28403688/quantification-of-early-cutaneous-manifestations-of-chronic-venous-insufficiency-by-automated-analysis-of-photographic-images-feasibility-and-technical-considerations
#9
François Becker, Patrice Fourgeau, Patrick H Carpentier, Amina Ouchène
We postulate that blue telangiectasia and brownish pigmentation at ankle level, early markers of chronic venous insufficiency, can be quantified for longitudinal studies of chronic venous disease in Caucasian people. Objectives and methods To describe a photographic technique specially developed for this purpose. The pictures were acquired using a dedicated photo stand to position the foot in a reproducible way, with a normalized lighting and acquisition protocol. The image analysis was performed with a tool developed using algorithms optimized to detect and quantify blue telangiectasia and brownish pigmentation and their relative surface in the region of interest...
January 1, 2017: Phlebology
https://www.readbyqxmd.com/read/28403266/laser-assisted-photodynamic-therapy-two-novel-protocols-for-enhanced-treatment-results
#10
Mary L Stevenson, Julie K Karen, Elizabeth K Hale
<p>Photodynamic therapy (PDT) uses a topical photosensitizing agent which is activated by a light source to cause destruction of specific cells. Commonly used for the treatment of actinic keratoses and photodamage, PDT can also be used for other conditions including acne and sebaceous hyperplasia. Here we report our experience with two treatment protocols. The first protocol utilizes laser assisted delivery of topical 5-aminolevulinic acid for enhanced efficacy of blue light photodynamic therapy in the treatment of actinic keratoses and photodamage...
April 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28382446/quantification-and-characterization-of-microplastics-in-blue-mussels-mytilus-edulis-protocol-setup-and-preliminary-data-on-the-contamination-of-the-french-atlantic-coast
#11
Nam Ngoc Phuong, Aurore Zalouk-Vergnoux, Abderrahmane Kamari, Catherine Mouneyrac, Frederic Amiard, Laurence Poirier, Fabienne Lagarde
Microplastics (MPs) constitute a main environmental issue due to their threat to marine organisms and so far to humans. The lack of a fast standard protocol in MP isolation and identification from living organisms bring to challenge for the science. In this paper, an optimized protocol using potassium hydroxide 10% (KOH 10%; m/v) for digestion of mussel soft tissues (Mytilus edulis) and multi-steps of sedimentation has been developed. Efficiency higher than 99.9% of organic and mineral matter elimination was shown by application on mussels sampled on the French Atlantic coast...
April 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28375931/a-comparison-of-the-detection-of-sentinel-lymph-nodes-using-indocyanine-green-and-near-infrared-fluorescence-imaging-versus-blue-dye-during-robotic-surgery-in-uterine-cancer
#12
Ane Gerda Eriksson, Anna Beavis, Robert A Soslow, Qin Zhou, Nadeem R Abu-Rustum, Ginger J Gardner, Oliver Zivanovic, Kara Long Roche, Yukio Sonoda, Mario M Leitao, Elizabeth L Jewell
OBJECTIVES: The objective of this study was to assess and compare the sentinel lymph node (SLN) detection rate with indocyanine green (ICG) and near-infrared fluorescence imaging versus blue dye using the robotic platform in patients with uterine cancer. METHODS: We identified all patients with uterine cancer undergoing SLN mapping using ICG or blue dye on the robotic platform from January 2011 to December 2013. Our institutional SLN algorithm and pathologic processing protocol were adhered to uniformly...
May 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28374972/ventilation-strategy-has-a-major-influence-on-remote-ischaemic-preconditioning-in-mice
#13
Sean M Davidson, Zhenhe He, Alex Dyson, Daniel I Bromage, Derek M Yellon
Whether oxygen should be administered acutely during ST-segment elevation myocardial infarction is debated. Despite this controversy, the possible influence of supplementary oxygen on animal models of ischaemia-reperfusion injury or cardioprotection is rarely considered. We used an in vivo mouse model of ischaemia and reperfusion to investigate the effect of ventilation with room air versus 100% oxygen. The coronary artery of anaesthetized mice was occluded for 40 min. followed by 2-hrs reperfusion. Infarct size was measured by tetrazolium staining and expressed as a percentage of area at risk, determined using Evan's blue...
April 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28363058/investigation-of-the-presence-of-atoxoplasma-spp-in-blue-crowned-laughingthrush-dryonastes-courtoisi-adults-and-neonates
#14
Florence Mohr, Martha Betson, Benoit Quintard
Between 1996 and 2013, 71 blue-crowned laughingthrush (Dryonastes courtoisi) chicks, a small passerine bird endemic to China, were born at Mulhouse Zoo in northeast France. None of them survived past 1 yr, and 82% died between 0 and 6 days old of an unidentified cause and despite an attempt to establish an artificial breeding protocol. Atoxoplasma spp., causing a disease known as systemic isosporosis, is a coccidian parasite that can infect several species of birds. Mulhouse's adult birds were suspected to be infected with Atoxoplasma spp...
March 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28362390/blue-hazard-free-candlelight-oled
#15
Jwo-Huei Jou, Meenu Singh, Yu-Ting Su, Shih-Hao Liu, Zhe-Kai He
A candlelight-style organic light emitting diode (OLED) is a human-friendly type of lighting because it is blue-hazard-free and has a low correlated color temperature (CCT) illumination. The low CCT lighting is deprived of high-energy blue radiation, and it can be used for a longer duration before causing retinal damage. This work presents the comprehensive protocols for the fabrication of blue-hazard-free candlelight OLEDs. The emission spectrum of the OLED was characterized by the maximum exposure time limit of the retina and the melatonin suppression sensitivity...
March 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28358607/radiation-therapy-of-periorbital-lymphoma-in-a-blue-and-gold-macaw-ara-ararauna
#16
Amy B Alexander, Lynn Griffin, Matthew S Johnston
An adult male blue and gold macaw (Ara ararauna) was presented for evaluation of a right periorbital mass. Results of diagnostic tests, including periorbital ultrasound, fine-needle aspirate, and cytologic examination, confirmed a diagnosis of periocular lymphoma. Radiographs were suggestive of disseminated disease but because of concerns about the patient's quality of life, localized radiation therapy to address the periorbital disease was pursued. Coarse-fractionated radiation therapy was initiated, and the mass regressed rapidly during the 3-week course of treatment...
March 2017: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28349398/flow-cytometry-for-the-isolation-and-characterization-of-rodent-meiocytes
#17
Adriana Geisinger, Rosana Rodríguez-Casuriaga
Molecular analyses in mammalian meiotic cells have been hindered by the difficulty in isolating stage-specific cell populations, and this is especially true for early meiotic prophase stages (leptotene and zygotene). Here, we describe a method for obtaining cells in different spermatogenic stages from rodents including lepto-zygotene meiocytes at very high purity levels. The procedure includes an approach for the mechanical disaggregation of the testicular tissue, staining with a vital, noncytotoxic dye that is excitable with a blue laser, isolation of the cell populations by flow sorting, and different alternative protocols for the collection of the sorted cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28337070/genomic-methods-and-microbiological-technologies-for-profiling-novel-and-extreme-environments-for-the-extreme-microbiome-project-xmp
#18
Scott Tighe, Ebrahim Afshinnekoo, Tara M Rock, Ken McGrath, Noah Alexander, Alexa McIntyre, Sofia Ahsanuddin, Daniela Bezdan, Stefan J Green, Samantha Joye, Sarah Stewart Johnson, Don A Baldwin, Nathan Bivens, Nadim Ajami, Joseph R Carmical, Ian Charold Herriott, Rita Colwell, Mohamed Donia, Jonathan Foox, Nick Greenfield, Tim Hunter, Jessica Hoffman, Joshua Hyman, Ellen Jorgensen, Diana Krawczyk, Jodie Lee, Shawn Levy, Natàlia Garcia-Reyero, Matthew Settles, Kelley Thomas, Felipe Gómez, Lynn Schriml, Nikos Kyrpides, Elena Zaikova, Jon Penterman, Christopher E Mason
The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques. The goals are multifaceted, including development and refinement of new techniques for the following: 1) the detection and characterization of novel microbes, 2) the evaluation of nucleic acid techniques for extremophilic samples, and 3) the identification and implementation of the appropriate bioinformatics pipelines...
March 10, 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28332232/paper-based-colorimetric-sensor-system-for-high-throughput-screening-of-c-h-borylation
#19
Han-Sung Kim, Min Sik Eom, Min Su Han, Sunwoo Lee
A paper-based colorimetric sensor system (PBCSS) was developed to detect the amount of bis(pinacolato)diboron (B2 Pin2 ) and applied as a high-throughput screening protocol in Ir-catalyzed C-H borylation. First, 96 ligands were screened for the borylation of benzene, and then 12 of them were selected and tested for five substrates. These reaction mixtures were spotted in the PBCSS, showing a blue-violet color. The value of the gray scale of each reaction was obtained from these colored spots and converted to the extent of conversion of B2 Pin2 ...
March 22, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28323589/preventing-high-altitude-cerebral-edema-in-rats-with-repurposed-anti-angiogenesis-pharmacotherapy
#20
Samantha Tarshis, Joanne Maltzahn, Zoe Loomis, David C Irwin
BACKGROUND: High altitude cerebral edema (HACE) is a fulminant, deadly, and yet still unpredictable brain disease. A new prophylactic treatment for HACE and its predecessor, acute mountain sickness (AMS), needs to be developed without the contraindications or adverse effect profiles of acetazolamide and dexamethasone. Since neovascularization signals are likely key contributors to HACE/AMS, our approach was to examine already existing anti-angiogenic drugs to inhibit potential initiating HACE pathway(s)...
December 1, 2016: Aerospace Medicine and Human Performance
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