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hair follicle generating in vitro

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https://www.readbyqxmd.com/read/27867768/induced-pluripotent-stem-cells-from-human-hair-follicle-keratinocytes-as-a-potential-source-for-in-vitro-hair-follicle-cloning
#1
Sheng Jye Lim, Shu Cheow Ho, Pooi Ling Mok, Kian Lee Tan, Alan H K Ong, Seng Chiew Gan
BACKGROUND: Human hair follicles are important for the renewal of new hairs and their development. The generation of induced pluripotent stem cells (iPSCs) from hair follicles is easy due to its accessibility and availability. The pluripotent cells derived from hair follicles not only have a higher tendency to re-differentiate into hair follicles, but are also more suited for growth in hair scalp tissue microenvironment. METHODS: In this study, human hair follicular keratinocytes were used to generate iPSCs, which were then further differentiated in vitro into keratinocytes...
2016: PeerJ
https://www.readbyqxmd.com/read/27831564/skin-derived-stem-cells-as-a-source-of-primordial-germ-cell-and-oocyte-like-cells
#2
REVIEW
Wei Ge, Shun-Feng Cheng, Paul W Dyce, Massimo De Felici, Wei Shen
The skin is a unique organ that contains a variety of stem cells for the maintenance of skin homeostasis and the repair of skin tissues following injury and disease. Skin-derived stem cells (SDSCs) constitute a heterogeneous population of stem cells generated in vitro from dermis, which can be cultured as spherical aggregates of cells in suspension culture. Under certain in vitro or in vivo conditions, SDSCs show multipotency and can generate a variety of neural, mesodermal, and endodermal cell types such as neurons, glia, fibroblasts, adipocytes, muscle cells, chondroblasts, osteoblats, and islet β-cell-like cells...
November 10, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27679975/multifaceted-role-of-hair-follicle-dermal-cells-in-bioengineered-skins
#3
C A Higgins, M Roger, R Hill, A S Ali-Khan, J Garlick, A M Christiano, C A B Jahoda
BACKGROUND: The method to generate bioengineered skin constructs was pioneered several decades ago, and nowadays these constructs are used regularly for the treatment of severe burns and non-healing wounds. Commonly, these constructs are comprised of skin fibroblasts within a collagen scaffold, forming the skin dermis, and stratified keratinocytes overlying this, forming the skin epidermis. In the past decade there has been a surge of interest in bioengineered skins, with researchers searching for alternative cell sources, or scaffolds, from which constructs can be established, and for more biomimetic equivalents with skin appendages...
September 28, 2016: British Journal of Dermatology
https://www.readbyqxmd.com/read/27652036/in-vitro-culture-of-rat-hair-follicle-stem-cells-on-rabbit-bladder-acellular-matrix
#4
Jia Li, Wenguang Wang, Hengqing An, Feng Wang, Mulati Rexiati, Yujie Wang
BACKGROUND: The aim of this work was to create a xenogeneic cell scaffold complex with rabbit bladder acellular matrix and rat hair follicle stem cells, to study the feasibility of construct tissue engineer bladder through biocompatibility of hair follicle stem cells and heterogeneous bladder acellular matrix. MATERIAL AND METHODS: New Zealand rabbit bladder acellular matrix was prepared. Scanning electron microscope and Masson staining were used to analyse the acellular material...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27612515/enhanced-expansion-and-sustained-inductive-function-of-skin-derived-precursor-cells-in-computer-controlled-stirred-suspension-bioreactors
#5
Natacha A Agabalyan, Breanna S Borys, Holly D Sparks, Kathryn Boon, Eko W Raharjo, Sepideh Abbasi, Michael S Kallos, Jeff Biernaskie
: : Endogenous dermal stem cells (DSCs) reside in the adult hair follicle mesenchyme and can be isolated and grown in vitro as self-renewing colonies called skin-derived precursors (SKPs). Following transplantation into skin, SKPs can generate new dermis and reconstitute the dermal papilla and connective tissue sheath, suggesting they could have important therapeutic value for the treatment of skin disease (alopecia) or injury. Controlled cell culture processes must be developed to efficiently and safely generate sufficient stem cell numbers for clinical use...
September 9, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27502902/chromatographic-method-for-clobetasol-propionate-determination-in-hair-follicles-and-in-different-skin-layers
#6
Tamara Ângelo, Marcílio S S Cunha-Filho, Guilherme M Gelfuso, Tais Gratieri
Clobetasol propionate (CLO) is a potent steroid used for the treatment of several dermatological diseases. Recent studies suggest its additional use in alopecia topical treatment, generating a demand for novel formulations with specific delivery into hair follicles. Hence, a selective analytical method for drug quantification in follicular structures and skin layers is required. For this, a simple HPLC-UV method was developed. Quantification was performed using a RP-C18 column (4.6 mm × 15 cm, 5 μm), with a mixture of methanol-acetonitrile-water (50:15:35 v/v) as mobile phase, a flow rate of 1...
August 8, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27462123/cd133-positive-dermal-papilla-derived-wnt-ligands-regulate-postnatal-hair-growth
#7
Linli Zhou, Kun Yang, April Carpenter, Richard A Lang, Thomas Andl, Yuhang Zhang
Active Wnt/β-catenin signaling in the dermal papilla (DP) is required for postnatal hair cycling. In addition, maintenance of the hair-inducing ability of DP cells in vitro requires external addition of Wnt molecules. However, whether DP cells are a critical source of Wnt ligands and induce both autocrine and paracrine signaling cascades to promote adult hair follicle growth and regeneration remains elusive. To address this question, we generated an animal model that allows inducible ablation of Wntless (Wls), a transmembrane Wnt exporter protein, in CD133-positive (CD133+) DP cells...
October 1, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27458264/hair-follicle-and-sebaceous-gland-de-novo-regeneration-with-cultured-epidermal-stem-cells-and-skin-derived-precursors
#8
Xiaoxiao Wang, Xusheng Wang, Jianjun Liu, Ting Cai, Ling Guo, Shujuan Wang, Jinmei Wang, Yanpei Cao, Jianfeng Ge, Yuyang Jiang, Edward E Tredget, Mengjun Cao, Yaojiong Wu
: : Stem cell-based organ regeneration is purported to enable the replacement of impaired organs in the foreseeable future. Here, we demonstrated that a combination of cultured epidermal stem cells (Epi-SCs) derived from the epidermis and skin-derived precursors (SKPs) was capable of reconstituting functional hair follicles and sebaceous glands (SG). When Epi-SCs and SKPs were mixed in a hydrogel and implanted into an excisional wound in nude mice, the Epi-SCs formed de novo epidermis along with hair follicles, and SKPs contributed to dermal papilla in the neogenic hair follicles...
July 25, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27053247/differentiation-of-hepatocytes-from-induced-pluripotent-stem-cells-derived-from-human-hair-follicle-mesenchymal-stem-cells
#9
Xu Shi, Shuang Lv, Xia He, Xiaomei Liu, Meiyu Sun, Meiying Li, Guangfan Chi, Yulin Li
Due to the limitations of organ donors and immune rejection in severe liver diseases, stem cell-based therapy presents a promising application for tissue repair and regeneration. As a novel cell source, mesenchymal stem cells separated from human hair follicles (HF-MSCs) are convenient to obtain and have no age limit. To date, the differentiation of HF-MSCs into hepatocytes has not been reported. In this study, we explored whether HF-MSCs and HF-MSC-derived-induced pluripotent stem cells (HF-iPS) could differentiate into hepatocytes in vitro...
October 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27051874/bioengineering-a-3d-integumentary-organ-system-from-ips-cells-using-an-in-vivo-transplantation-model
#10
Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh-Ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa, Takashi Tsuji
The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis...
April 2016: Science Advances
https://www.readbyqxmd.com/read/26912421/pharmacological-bace1-and-bace2-inhibition-induces-hair-depigmentation-by-inhibiting-pmel17-processing-in-mice
#11
Derya R Shimshek, Laura H Jacobson, Carine Kolly, Natasa Zamurovic, Kamal Kumar Balavenkatraman, Laurent Morawiec, Robert Kreutzer, Juliane Schelle, Mathias Jucker, Barbara Bertschi, Diethilde Theil, Annabelle Heier, Karine Bigot, Karen Beltz, Rainer Machauer, Irena Brzak, Ludovic Perrot, Ulf Neumann
Melanocytes of the hair follicle produce melanin and are essential in determining the differences in hair color. Pigment cell-specific MELanocyte Protein (PMEL17) plays a crucial role in melanogenesis. One of the critical steps is the amyloid-like functional oligomerization of PMEL17. Beta Site APP Cleaving Enzyme-2 (BACE2) and γ-secretase have been shown to be key players in generating the proteolytic fragments of PMEL17. The β-secretase (BACE1) is responsible for the generation of amyloid-β (Aβ) fragments in the brain and is therefore proposed as a therapeutic target for Alzheimer's disease (AD)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26820528/protopanaxatirol-type-ginsenoside-re-promotes-cyclic-growth-of-hair-follicles-via-inhibiting-transforming-growth-factor-%C3%AE-signaling-cascades
#12
Zheng Li, Seung-Wook Ryu, Jungsul Lee, Kyungsun Choi, Sunchang Kim, Chulhee Choi
Ginsenosides, the major bio-active ingredients included in Panax ginseng, have been known for the hair growth activity and used to treat patients who suffer from hair loss; however, the detailed mechanisms of this action are still largely unknown. This study was conducted to investigate the molecular and cellular mechanisms responsible for hair growth promoting effect of ginsenoside Re (GRe) in vitro and in vivo. Different doses of minoxidil and GRe were administered topically to the back regions of nude mice for up to 45 days, and hair shaft length and hair cycles were determined for hair promoting activities...
February 19, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26788537/fam83h-null-mice-support-a-neomorphic-mechanism-for-human-adhcai
#13
Shih-Kai Wang, Yuanyuan Hu, Jie Yang, Charles E Smith, Amelia S Richardson, Yasuo Yamakoshi, Yuan-Ling Lee, Figen Seymen, Mine Koruyucu, Koray Gencay, Moses Lee, Murim Choi, Jung-Wook Kim, Jan C-C Hu, James P Simmer
Truncation mutations in FAM83H (family with sequence similarity 83, member H) cause autosomal dominant hypocalcified amelogenesis imperfecta (ADHCAI), but little is known about FAM83H function and the pathogenesis of ADHCAI. We recruited three ADHCAI families and identified two novel (p.Gln457*; p.Lys639*) and one previously documented (p.Q452*) disease-causing FAM83H mutations. We generated and characterized Fam83h-knockout/lacZ-knockin mice. Surprisingly, enamel thickness, density, Knoop hardness, morphology, and prism patterns were similar in Fam83h (+/+), Fam83h (+/-), and Fam83h (-/-) mice...
January 2016: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/26118627/wnt1a-maintains-characteristics-of-dermal-papilla-cells-that-induce-mouse-hair-regeneration-in-a-3d-preculture-system
#14
Liang Dong, Haojie Hao, Jiejie Liu, Chuan Tong, Dongdong Ti, Deyun Chen, Li Chen, Meirong Li, Huiling Liu, Xiaobing Fu, Weidong Han
Hair follicle morphogenesis and regeneration depend on intensive but well-orchestrated interactions between epithelial and mesenchymal components. Therefore, an alternative strategy to reproduce the process of epithelial-mesenchymal interaction in vitro could use a 3D system containing appropriate cell populations. The 3D air-liquid culture system for reproducibly generating hair follicles from dissociated epithelial and dermal papilla (DP) cells combined with a collagen-chitosan scaffold is described in this study...
June 29, 2015: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/26053520/generation-of-induced-pluripotent-stem-cells-from-bovine-epithelial-cells-and-partial-redirection-toward-a-mammary-phenotype-in-vitro
#15
Diego Cravero, Eugenio Martignani, Silvia Miretti, Paulo Accornero, Alfredo Pauciullo, Ruchi Sharma, Francesco Xavier Donadeu, Mario Baratta
In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal production has not been investigated. Herein, we investigated the potential application of iPSCs in dairy production by generating bovine iPSCs and establishing their ability to generate mammary epithelial tissue...
June 2015: Cellular Reprogramming
https://www.readbyqxmd.com/read/25948482/feasibility-of-human-hair-follicle-derived-mesenchymal-stem-cells-cultispher-%C3%A2-g-constructs-in-regenerative-medicine
#16
Pengdong Li, Feilin Liu, Chunling Wu, Wenyue Jiang, Guifang Zhao, Li Liu, Tingting Bai, Li Wang, Yixu Jiang, Lili Guo, Xiaojuan Qi, Junna Kou, Ruirui Fan, Deshun Hao, Shaowei Lan, Yulin Li, Jin Yu Liu
The use of human mesenchymal stem cells (hMSCs) in cell therapies has increased the demand for strategies that allow efficient cell scale-up. Preliminary data on the three-dimensional (3D) spinner culture describing the potential use of microcarriers for hMSCs culture scale-up have been reported. We exploited a rich source of autologous stem cells (human hair follicle) and demonstrated the robust in vitro long-term expansion of human hair follicle-derived mesenchymal stem cells (hHF-MSCs) by using CultiSpher(®)-G microcarriers...
October 2015: Cell and Tissue Research
https://www.readbyqxmd.com/read/25437427/partial-maintenance-and-long-term-expansion-of-murine-skin-epithelial-stem-cells-by-wnt-3a-in-vitro
#17
Yukiteru Ouji, Shigeaki Ishizaka, Fukumi Nakamura-Uchiyama, Daisuke Okuzaki, Masahide Yoshikawa
CD49f(+)CD34(+) cells, a skin epithelial stem cell (EpSC)-rich population, were prepared from adult mouse skin and cultured in the presence of Wnt-3a without feeder cells. CD34 expression was retained in about 10% of the cells, which had proliferated about 1,000-fold by day 10, although completely lost by day 14. CD49f(+)CD34(+) cells sorted on day 10 retained canonical Wnt-responsiveness, proliferated markedly in the presence of Wnt-3a, maintained undifferentiated epithelial cell marker expression, and promoted hair follicle development in vivo...
June 2015: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/25180455/the-vitamin-d-receptor-is-required-for-activation-of-cwnt-and-hedgehog-signaling-in-keratinocytes
#18
Thomas S Lisse, Vaibhav Saini, Hengguang Zhao, Hilary F Luderer, Francesca Gori, Marie B Demay
Alopecia (hair loss) in vitamin D receptor (VDR)-null mice is due to absence of ligand-independent actions of the VDR that are required for initiation of postmorphogenic hair cycles. Investigations were undertaken to determine whether the VDR is required for the induction of signaling pathways that play an important role in this process. The induction of cWnt and hedgehog target genes that characterizes early anagen was found to be dramatically attenuated in VDR(-/-) mice, relative to wild-type (WT) mice. To determine whether this reflects impaired responsiveness to cWnt ligands, in vitro studies were performed in primary keratinocytes...
October 2014: Molecular Endocrinology
https://www.readbyqxmd.com/read/25161315/dermal-papilla-cells-improve-the-wound-healing-process-and-generate-hair-bud-like-structures-in-grafted-skin-substitutes-using-hair-follicle-stem-cells
#19
Gustavo José Leirós, Ana Gabriela Kusinsky, Hugo Drago, Silvia Bossi, Flavio Sturla, María Lía Castellanos, Inés Yolanda Stella, María Eugenia Balañá
Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs)...
October 2014: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/25122137/alteration-of-skin-wound-healing-in-keratinocyte-specific-mediator-complex-subunit-1-null-mice
#20
Fumihito Noguchi, Takeshi Nakajima, Shigeki Inui, Janardan K Reddy, Satoshi Itami
MED1 (Mediator complex subunit 1) is a co-activator of various transcription factors that function in multiple transcriptional pathways. We have already established keratinocyte-specific MED1 null mice (Med1(epi-/-)) that develop epidermal hyperplasia. Herein, to investigate the function(s) of MED1 in skin wound healing, full-thickness skin wounds were generated in Med1(epi-/-) and age-matched wild-type mice and the healing process was analyzed. Macroscopic wound closure and the re-epithelialization rate were accelerated in 8-week-old Med1(epi-/-) mice compared with age-matched wild-type mice...
2014: PloS One
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