keyword
https://read.qxmd.com/read/38452278/efficient-generation-of-skin-organoids-from-pluripotent-cells-via-defined-extracellular-matrix-cues-and-morphogen-gradients-in-a-spindle-shaped-microfluidic-device
#1
JOURNAL ARTICLE
Cristina Quílez, Eun Y Jeon, Alberto Pappalardo, Pooja Pathak, Hasan E Abaci
Pluripotent stem cell-derived skin organoids (PSOs) recently emerged as a developmental skin model that is self-organized into multiple components, such as hair follicles. Despite their impressive cellular and anatomical complexity, PSOs are currently generated in the absence of 3D extracellular matrix (ECM) signals and have several major limitations, including an inverted anatomy (e.g., epidermis inside/dermis outside). In this work, we established a method to generate PSOs effectively in a chemically-defined 3D ECM environment...
March 7, 2024: Advanced Healthcare Materials
https://read.qxmd.com/read/38428092/development-of-pluripotent-stem-cell-derived-epidermal-organoids-that-generate-effective-extracellular-vesicles-in-skin-regeneration
#2
JOURNAL ARTICLE
Sojung Kwak, Cho Lok Song, Jinhyuk Lee, Sungyeon Kim, Seungyoon Nam, Young-Jun Park, Jungwoon Lee
Cellular skin substitutes such as epidermal constructs have been developed for various applications, including wound healing and skin regeneration. These cellular models are mostly derived from primary cells such as keratinocytes and fibroblasts in a two-dimensional (2D) state, and further development of three-dimensional (3D) cultured organoids is needed to provide insight into the in vivo epidermal phenotype and physiology. Here, we report the development of epidermal organoids (EpiOs) generated from induced pluripotent stem cells (iPSCs) as a novel epidermal construct and its application as a source of secreted biomolecules recovered by extracellular vesicles (EVs) that can be utilized for cell-free therapy of regenerative medicine...
February 23, 2024: Biomaterials
https://read.qxmd.com/read/38277391/rat-hair-follicle-associated-pluripotent-hap-stem-cells-can-differentiate-into-atrial-or-ventricular-cardiomyocytes-in-culture-controlled-by-specific-supplementation
#3
JOURNAL ARTICLE
Nanako Takaoka, Michiko Yamane, Ayami Hasegawa, Koya Obara, Kyoumi Shirai, Ryoichi Aki, Hiroyasu Hatakeyama, Yuko Hamada, Nobuko Arakawa, Manabu Tanaka, Robert M Hoffman, Yasuyuki Amoh
There has been only limited success to differentiate adult stem cells into cardiomyocyte subtypes. In the present study, we have successfully induced beating atrial and ventricular cardiomyocytes from rat hair-follicle-associated pluripotent (HAP) stem cells, which are adult stem cells located in the bulge area. HAP stem cells differentiated into atrial cardiomyocytes in culture with the combination of isoproterenol, activin A, bone morphogenetic protein 4 (BMP4), basic fibroblast growth factor (bFGF), and cyclosporine A (CSA)...
2024: PloS One
https://read.qxmd.com/read/38189899/human-induced-pluripotent-stem-cell%C3%A2-derived-keratinocytes-as-therapeutic-option-in-vitiligo
#4
JOURNAL ARTICLE
Babak Arjmand, Elahe Bahrami-Vahdat, Sepideh Alavi-Moghadam, Rasta Arjmand, Mostafa Rezaei-Tavirani, Nazli Namazi, Bagher Larijani
Vitiligo is a skin condition affecting 1% of the global population, causing non-scaly, chalky-white macules on the skin and hair. It is caused by the pathologic destruction of melanocytes, which produce melanin. Research has focused on the abnormalities of melanocytes and their interaction with neighboring keratinocytes. Current treatments are mainly immunosuppressive drugs and UV radiation, which are scarce and ineffective. To treat vitiligo, regenerative medicine techniques, such as cell-based and cell-free methods, are recommended...
January 9, 2024: Methods in Molecular Biology
https://read.qxmd.com/read/38167128/genetic-correction-of-induced-pluripotent-stem-cells-from-a-dfna36-patient-results-in-morphologic-and-functional-recovery-of-derived-hair-cell-like-cells
#5
JOURNAL ARTICLE
Yi Luo, Kaiwen Wu, Xiaolong Zhang, Hongyang Wang, Qiuju Wang
BACKGROUND: TMC1 is one of the most common deafness genes causing DFNA36. Patient-derived human induced pluripotent stem cells (iPSCs) provide an opportunity to modelling diseases. TMC1 p.M418K mutation in human is orthologous to Beethoven mice. Here, we investigated the differentiation, morphology and electrophysiological properties of hair cell-like cells (HC-like cells) derived from DFNA36 patient. METHODS: Inner ear HC-like cells were induced from iPSCs derived from DFNA36 (TMC1 p...
January 2, 2024: Stem Cell Research & Therapy
https://read.qxmd.com/read/38044307/development-of-physiologically-relevant-skin-organoids-from-human-induced-pluripotent-stem-cells
#6
JOURNAL ARTICLE
Abbas Shafiee, Jane Sun, Imaan A Ahmed, Felicia Phua, Gustavo R Rossi, Cheng-Yu Lin, Fernando Souza-Fonseca-Guimaraes, Ernst J Wolvetang, Jason Brown, Kiarash Khosrotehrani
The development of skin organs for studying developmental pathways, modeling diseases, or regenerative medicine purposes is a major endeavor in the field. Human induced pluripotent stem cells (hiPSCs) are successfully used to derive skin cells, but the field is still far from meeting the goal of creating skin containing appendages, such as hair follicles and sweat glands. Here, the goal is to generate skin organoids (SKOs) from human skin fibroblast or placental CD34+ cell-derived hiPSCs. With all three hiPSC lines, complex SKOs with stratified skin layers and pigmented hair follicles are generated with different efficacies...
December 3, 2023: Small
https://read.qxmd.com/read/38043402/nadh-improves-aif-dimerization-and-inhibits-apoptosis-in-ipscs-derived-neurons-from-patients-with-auditory-neuropathy-spectrum-disorder
#7
JOURNAL ARTICLE
Yue Qiu, Hongyang Wang, Huaye Pan, Xue Ding, Jing Guan, Qianqian Zhuang, Kaiwen Wu, Zhaoying Lei, Huajian Cai, Yufei Dong, Hui Zhou, Aifu Lin, Qiuju Wang, Qingfeng Yan
Auditory neuropathy spectrum disorder (ANSD) is a hearing impairment involving disruptions to inner hair cells (IHCs), ribbon synapses, spiral ganglion neurons (SGNs), and/or the auditory nerve itself. The outcomes of cochlear implants (CI) for ANSD are variable and dependent on the location of lesion sites. Discovering a potential therapeutic agent for ANSD remains an urgent requirement. Here, 293T stable transfection cell lines and patient induced pluripotent stem cells (iPSCs)-derived auditory neurons carrying the apoptosis inducing factor (AIF) p...
November 22, 2023: Hearing Research
https://read.qxmd.com/read/37995436/generation-of-an-induced-pluripotent-stem-cell-ipsc-line-sdqlchi056-a-from-a-patient-with-nicolaides-baraitser-syndrome-carrying-a-mutation-in-smarca2-gene
#8
JOURNAL ARTICLE
Xiaomeng Yang, Chunmei Yu, Min Gao, Yi Liu, Yuanxiang Liu
Nicolaides-Baraitser syndrome (NCBRS) is characterized by severe mental retardation, early-onset seizures, short stature, dysmorphic facial features, and sparse hair. Heterozygous mutations in the SMARCA2 gene are the most common cause of NCBRS. Here, we generated an induced pluripotent stem cell line SDQLCHi056-A from a five-year-old girl with NCBRS caused by heterozygous mutation (c.3592 G > A, p.V1198M) in the SMARCA2 gene (OMIM#601358). The established iPSC line was validated by pluripotency markers, original gene mutation and demonstrated trilineage differentiation potential in vitro...
November 14, 2023: Stem Cell Research
https://read.qxmd.com/read/37962101/natural-history-of-knq4-p-g285s-related-hearing-loss-construction-of-ipsc-and-mouse-model
#9
JOURNAL ARTICLE
Xiaolong Zhang, Tao Shi, Jin Li, Xiaonan Wu, Kaili Wu, Danyang Li, Dayong Wang, Jing Guan, Hongyang Wang
OBJECTIVE: KCNQ4 is one of the most common disease-causing genes involved in autosomal dominant non-syndromic hearing loss. We previously found that patients with KCNQ4 p.G285S exhibited a much more rapid deterioration in hearing loss than those with other KCNQ4 variants. To determine the rate of hearing loss and assess the disease for further analysis, we performed a long-term follow-up of these patients and generated patient-derived induced pluripotent stem cells (iPSCs), and a mouse model...
November 14, 2023: Laryngoscope
https://read.qxmd.com/read/37847179/the-role-of-mesenchymal-stem-cells-in-hair-regeneration-and-hair-cycle
#10
JOURNAL ARTICLE
Cong Ma, Ming Cheng, Yan Wu, Xuegang Xu
The health of hair is directly related to people's health and appearance. Hair has key physiological functions, including skin protection and temperature regulation. Hair follicle is a vital mini-organ that directly impacts hair growth. Besides, various signaling pathways and molecules regulate the growth cycle transition of hair follicles. Hair and its regeneration studies have attracted much interest in recent years with the increasing rate of alopecia. Mesenchymal stem cells (MSCs), as pluripotent stem cells, can differentiate into fat, bone, and cartilage and stimulate regeneration and immunological regulation...
October 17, 2023: Stem Cells and Development
https://read.qxmd.com/read/37845538/-organoids-the-key-to-novel-therapies-for-the-inner-ear-german-version
#11
REVIEW
Marc Diensthuber, Timo Stöver
The sensitivity and the complexity of the human inner ear in conjunction with the lack of regenerative capacity are the main reasons for hearing loss and tinnitus. Progress in the development of protective and regenerative therapies for the inner ear often failed in the past not least due to the fact that no suitable model systems for cell biological and pharmacological in vitro studies were available. A novel technology for creating "mini-organs", so-called organoids, could solve this problem and has now also reached inner ear research...
October 16, 2023: HNO
https://read.qxmd.com/read/37796037/mapping-oto-pharyngeal-development-in-a-human-inner-ear-organoid-model
#12
JOURNAL ARTICLE
Matthew R Steinhart, Wouter H van der Valk, Daniel Osorio, Sara A Serdy, Jingyuan Zhang, Carl Nist-Lund, Jin Kim, Cynthia Moncada-Reid, Liang Sun, Jiyoon Lee, Karl R Koehler
Inner ear development requires the coordination of cell types from distinct epithelial, mesenchymal and neuronal lineages. Although we have learned much from animal models, many details about human inner ear development remain elusive. We recently developed an in vitro model of human inner ear organogenesis using pluripotent stem cells in a 3D culture, fostering the growth of a sensorineural circuit, including hair cells and neurons. Despite previously characterizing some cell types, many remain undefined. This study aimed to chart the in vitro development timeline of the inner ear organoid to understand the mechanisms at play...
October 1, 2023: Development
https://read.qxmd.com/read/37739763/applications-of-human-pluripotent-stem-cell-derived-skin-organoids-in-dermatology
#13
JOURNAL ARTICLE
Aaron Gabriel W Sandoval, Kelly Y Gim, Jennifer T Huang, Karl R Koehler
Pluripotent stem cells have the potential to become any cell type, and recently, they have been used to create organoids that can recapitulate several pertinent features of human organs. Skin organoids have been developed that possess many of the crucial accessory organs, including hair follicles, sebaceous glands, nerves, fat, and melanocytes. These skin organoids present the opportunity to study skin development and disease as well as perform screens to identify new drug candidates. In the future, skin organoids might augment clinical practice by serving as source material for transplantation to treat wounds or other conditions...
October 2023: Journal of Investigative Dermatology
https://read.qxmd.com/read/37737178/induced-pluripotent-stem-cells-ex-vivo-models-for-human-diseases-due-to-mitochondrial-dna-mutations
#14
REVIEW
Chao Chen, Min-Xin Guan
Mitochondria are essential organelles for cellular metabolism and physiology in eukaryotic cells. Human mitochondria have their own genome (mtDNA), which is maternally inherited with 37 genes, encoding 13 polypeptides for oxidative phosphorylation, and 22 tRNAs and 2 rRNAs for translation. mtDNA mutations are associated with a wide spectrum of degenerative and neuromuscular diseases. However, the pathophysiology of mitochondrial diseases, especially for threshold effect and tissue specificity, is not well understood and there is no effective treatment for these disorders...
September 22, 2023: Journal of Biomedical Science
https://read.qxmd.com/read/37612404/author-correction-generation-and-characterization-of-hair-bearing-skin-organoids-from-human-pluripotent-stem-cells
#15
Jiyoon Lee, Wouter H van der Valk, Sara A Serdy, CiCi Deakin, Jin Kim, Anh Phuong Le, Karl R Koehler
No abstract text is available yet for this article.
August 23, 2023: Nature Protocols
https://read.qxmd.com/read/37540158/gene-expression-profile-of-hipsc-derived-cells-differentiated-with-growth-factors-forskolin-and-conditioned-medium-from-human-adrenocortical-cell-line
#16
JOURNAL ARTICLE
Ewelina Stelcer, Karol Jopek, Małgorzata Blatkiewicz, Anna Olechnowicz, Kacper Kamiński, Marta Szyszka, Wiktoria Maria Suchorska, Marcin Ruciński
BACKGROUND: Adrenocortical carcinoma (ACC) affects approx. 2 in 1,000,000 individuals in the USA, and is more common in females than males. Adrenocortical carcinoma often presents with severe symptoms, such as abdominal pain, high blood pressure, acne, hair overgrowth, and voice deepening. OBJECTIVES: Research on ACC constitutes a large body of published data. There is an increased need for easy access to ACC-derived biological material. Moreover, there are limited numbers of human cell lines available...
August 4, 2023: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://read.qxmd.com/read/37446376/hypoxia-treatment-of-adipose-mesenchymal-stem-cells-promotes-the-growth-of-dermal-papilla-cells-via-hif-1%C3%AE-and-erk1-2-signaling-pathways
#17
JOURNAL ARTICLE
Qing Wang, Mei Zhou, Hongyan Zhang, Zhuang Hou, Dongjun Liu
Dermal papilla cells (DPCs) cultured in vitro induce hair follicle formation. Using a hypoxic microenvironment to culture adipose mesenchymal stem cells (ADSCs) can promote hair follicle growth. However, the exact molecular mechanisms underlying this process remain unclear. In this study, ADSCs and DPCs from Arbas Cashmere goats were used. A hypoxic microenvironment promoted the proliferation of ADSCs and increased the pluripotency of ADSCs. The growth factors vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were upregulated in ADSCs in the hypoxia-conditioned medium (Hypo-cm)...
July 7, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37429584/efficient-establishment-of-an-optimized-culture-condition-for-cashmere-goat-primary-hair-follicle-stem-cells
#18
JOURNAL ARTICLE
Yan Li, Miaohan Jin, Yawei Gao, Lijin Lu, Jing Cao, Yao Liu, Yulin Chen, Xiaolong Wang
Hair follicle stem cells (HFSCs) are an important basis for hair follicle morphogenesis and hair cycle growth. This cell type also represents an excellent model for studying the gene function and molecular regulation of the hair growth cycle, including proliferation, differentiation, and apoptosis. Basically, the functional investigation of hair growth-regulating genes demands a sufficient amount of HFSCs. However, efficient propagation of HFSCs in goats is a challenging process under the current culture conditions...
July 10, 2023: Journal of Animal Science
https://read.qxmd.com/read/37419105/generating-high-fidelity-cochlear-organoids-from-human-pluripotent-stem-cells
#19
JOURNAL ARTICLE
Stephen T Moore, Takashi Nakamura, Jing Nie, Alexander J Solivais, Isabel Aristizábal-Ramírez, Yoshitomo Ueda, Mayakannan Manikandan, V Shweta Reddy, Daniel R Romano, John R Hoffman, Benjamin J Perrin, Rick F Nelson, Gregory I Frolenkov, Susana M Chuva de Sousa Lopes, Eri Hashino
Mechanosensitive hair cells in the cochlea are responsible for hearing but are vulnerable to damage by genetic mutations and environmental insults. The paucity of human cochlear tissues makes it difficult to study cochlear hair cells. Organoids offer a compelling platform to study scarce tissues in vitro; however, derivation of cochlear cell types has proven non-trivial. Here, using 3D cultures of human pluripotent stem cells, we sought to replicate key differentiation cues of cochlear specification. We found that timed modulations of Sonic Hedgehog and WNT signaling promote ventral gene expression in otic progenitors...
July 6, 2023: Cell Stem Cell
https://read.qxmd.com/read/37365177/impaired-aif-chchd4-interaction-and-mitochondrial-calcium-overload-contribute-to-auditory-neuropathy-spectrum-disorder-in-patient-ipsc-derived-neurons-with-aifm1-variant
#20
JOURNAL ARTICLE
Yue Qiu, Hongyang Wang, Mingjie Fan, Huaye Pan, Jing Guan, Yangwei Jiang, Zexiao Jia, Kaiwen Wu, Hui Zhou, Qianqian Zhuang, Zhaoying Lei, Xue Ding, Huajian Cai, Yufei Dong, Lei Yan, Aifu Lin, Yong Fu, Dong Zhang, Qingfeng Yan, Qiuju Wang
Auditory neuropathy spectrum disorder (ANSD) is a hearing impairment caused by dysfunction of inner hair cells, ribbon synapses, spiral ganglion neurons and/or the auditory nerve itself. Approximately 1/7000 newborns have abnormal auditory nerve function, accounting for 10%-14% of cases of permanent hearing loss in children. Although we previously identified the AIFM1 c.1265 G > A variant to be associated with ANSD, the mechanism by which ANSD is associated with AIFM1 is poorly understood...
June 26, 2023: Cell Death & Disease
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