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pluripotent stem cell hair cell

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https://www.readbyqxmd.com/read/29740017/atoh1-rfx1-rfx3-transcription-factors-facilitate-the-differentiation-and-characterisation-of-inner-ear-hair-cell-like-cells-from-patient-specific-induced-pluripotent-stem-cells-harbouring-a8344g-mutation-of-mitochondrial-dna
#1
Yen-Chun Chen, Chia-Ling Tsai, Yau-Huei Wei, Yu-Ting Wu, Wei-Ting Hsu, Hung-Ching Lin, Yi-Chao Hsu
Degeneration or loss of inner ear hair cells (HCs) is irreversible and results in sensorineural hearing loss (SHL). Human-induced pluripotent stem cells (hiPSCs) have been employed in disease modelling and cell therapy. Here, we propose a transcription factor (TF)-driven approach using ATOH1 and regulatory factor of x-box (RFX) genes to generate HC-like cells from hiPSCs. Our results suggest that ATOH1/RFX1/RFX3 could significantly increase the differentiation capacity of iPSCs into MYO7AmCherry -positive cells, upregulate the mRNA expression levels of HC-related genes and promote the differentiation of HCs with more mature stereociliary bundles...
April 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29700975/use-of-homeobox-gene-expression-patterns-to-determine-anatomical-regions-of-origin-for-body-surface-tissues-derived-from-adult-mice
#2
Toru Okubo, Ryuhei Hayashi, Shun Shibata, Yuji Kudo, Yoichi Honma, Kohji Nishida
Anatomical regions of the skin have distinct functions and anatomical characteristics, including thicker or thinner epidermis, more or fewer hair follicles, and lighter or darker skin. For a better therapeutic outcome of skin transplantation, site-specific characteristics of grafted tissues need to be taken into account in terms of their functionality and beauty. However, there is no method for evaluating positional information of epidermal cells. Homeobox genes are expressed along the anterior-posterior axis and direct the body plan in the animal development process...
April 26, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29572803/nerve-growth-and-interaction-in-gelfoam-%C3%A2-histoculture-a-nervous-system-organoid
#3
Robert M Hoffman, Sumiyuki Mii, Jennifer Duong, Yasuyuki Amoh
Nestin-expressing hair follicle-associated pluripotent (HAP) stem cells reside mainly in the bulge area (BA) of the hair follicle but also in the dermal papilla (DP). The BA appears to be origin of HAP stem cells. Long-term Gelfoam® histoculture was established of whiskers isolated from transgenic mice, in which there is nestin-driven green fluorescent protein (ND-GFP). HAP stem cells trafficked from the BA toward the DP area and extensively grew out onto Gelfoam® forming nerve-like structures. These fibers express the neuron marker β-III tubulin-positive fibers and consisted of ND-GFP-expressing cells and extended up to 500 mm from the whisker nerve stump in Gelfoam® histoculture...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29572802/hair-follicle-associated-pluripotent-hap-stem-cells-in-gelfoam-%C3%A2-histoculture-for-use-in-spinal-cord-repair
#4
Fang Liu, Robert M Hoffman
The stem cell marker, nestin, is expressed in the hair follicle, both in cells in the bulge area (BA) and the dermal papilla (DP). Nestin-expressing hair follicle-associated-pluripotent (HAP) stem cells of both the BA and DP have been previously shown to be able to form neurons, heart muscle cells, and other non-follicle cell types. The ability of the nestin-expressing HAP stem cells from the BA and DP to repair spinal cord injury was compared. Nestin-expressing HAP stem cells from both the BA and DP grew very well on Gelfoam® ...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29572801/hair-shaft-growth-in-gelfoam-%C3%A2-histoculture-of-skin-and-isolated-hair-follicles
#5
Robert M Hoffman, Lingna Li, Wenluo Cao
Human scalp skin with abundant hair follicles in various stages of the hair growth cycle was histocultured for up to 40 days on Gelfoam® at the air/liquid interface. The anagen hair follicles within the histoculture scalp skin produced growing hair shafts. Hair follicles could continue their cycle in histoculture; for example, apparent spontaneous catagen induction was observed both histologically and by the actual regression of the hair follicle. In addition, vellus follicles were shown to be viable at day 40 after initiation of culture...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29572789/3d-sponge-matrix-histoculture-an-overview
#6
Robert M Hoffman
Three-dimensional cell culture and tissue culture (histoculture) is much more in vivo-like than 2D culture on plastic. Three-dimensional culture allows investigation of crucial events in tumor biology such as drug response, proliferation and cell cycle progression, cancer cell migration, invasion, metastasis, immune response, and antigen expression that mimic in vivo conditions. Three-dimensional sponge-matrix histoculture maintains the in vivo phenotype, including the formation of differentiated structures of normal and malignant tissues, perhaps due to cells maintaining their natural shape in a sponge-gel matrix such as Gelfoam® ...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29541960/the-role-of-sox9-in-maintaining-the-characteristics-and-pluripotency-of-arbas-cashmere-goat-hair-follicle-stem-cells
#7
Nimantana He, Zhenguo Dong, Dapeng Tai, Hao Liang, Xudong Guo, Ming Cang, Dongjun Liu
In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere goat hair follicle, and also positively expressed in both nucleus and cytoplasm of the gHFSCs. When the cells were transfected using Sox9-shRNA, cell growth slowed down and the expression of related genes decreased significantly, cell cycle was abnormal, while the expression of terminal differentiation marker loricrin was markedly increased; cells lost the typical morphology of HFSCs; the mRNA and protein expression of gHFSCs markers and stem cell pluripotency associated factors were all significantly decreased; the expression of Wnt signaling pathway genes LEF1, TCF1,c-Myc were significantly changed...
March 14, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29429517/in-vitro-identification-of-a-stem-cell-population-from-canine-hair-follicle-bulge-region
#8
Raquel V G de Castro, Mariana R Tavares, Fabiana F Bressan, Naira C G Pieri, Amanda Baracho Trindade Hill, Aline F Souza, Nathan da R N Cruz, Daniele S Martins, Carlos E Ambrósio, Flávio V Meirelles, Joaquim M Garcia
Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2- testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ)...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29298425/hair-follicle-development-in-mouse-pluripotent-stem-cell-derived-skin-organoids
#9
Jiyoon Lee, Robert Bӧscke, Pei-Ciao Tang, Byron H Hartman, Stefan Heller, Karl R Koehler
The mammalian hair follicle arises during embryonic development from coordinated interactions between the epidermis and dermis. It is currently unclear how to recapitulate hair follicle induction in pluripotent stem cell cultures for use in basic research studies or in vitro drug testing. To date, generation of hair follicles in vitro has only been possible using primary cells isolated from embryonic skin, cultured alone or in a co-culture with stem cell-derived cells, combined with in vivo transplantation...
January 2, 2018: Cell Reports
https://www.readbyqxmd.com/read/29285614/the-effects-of-quinine-on-neurophysiological-properties-of-dopaminergic-neurons
#10
Li Zou, Yingchao Xue, Michael Jones, Thomas Heinbockel, Mingyao Ying, Xiping Zhan
Quinine is an antimalarial drug that is toxic to the auditory system by commonly inducing hearing loss and tinnitus, presumably due to its ototoxic effects on disruption of cochlear hair cells and blockade of ion channels of neurons in the auditory system. To a lesser extent, quinine also causes ataxia, tremor, and dystonic reactions. As dopaminergic neurons are implicated to play a role in all of these diseases, we tested the toxicity of quinine on induced dopaminergic (iDA) neurons derived from human pluripotent stem cells (iPSCs) and primary dopaminergic (DA) neurons of substantia nigra from mice brain slices...
December 29, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/29174977/generation-of-an-induced-pluripotent-stem-cell-line-from-a-39-year-old-female-patient-with-severe-to-profound-non-syndromic-sensorineural-hearing-loss-and-a-a1555g-mutation-in-the-mitochondrial-mtrnr1-gene
#11
Yu-Hung Hsu, Yu-Ting Wu, Ching-Ying Huang, Ming-Ching Ho, Yu-Che Cheng, Shih-Han Syu, Huai-En Lu, Yen-Chun Chen, Chia-Ling Tsai, Hung-Ching Lin, Yau-Huei Wei, Yi-Chao Hsu, Patrick C H Hsieh
Sensorineural hearing loss (SNHL) is a prevalent form of deafness commonly arising from damage to the cochlear sensory hair cells and degeneration of the spiral ganglion neurons. In this study, Sendai virus was used to generate an induced pluripotent stem cell (iPSC) line from a 39-year-old female patient diagnosed with severe-to-profound, non-syndromic SNHL. The patient also carries a A1555G mutation in the mitochondrial 12S ribosome RNA gene (MTRNR1). This iPSC line was verified to express pluripotent markers, possess normal karyotype, harbor the specific mutation and demonstrated the capacity to differentiate into three germ layers...
December 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29107558/esrp1-mutations-cause-hearing-loss-due-to-defects-in-alternative-splicing-that-disrupt-cochlear-development
#12
Alex M Rohacek, Thomas W Bebee, Richard K Tilton, Caleb M Radens, Chris McDermott-Roe, Natoya Peart, Maninder Kaur, Michael Zaykaner, Benjamin Cieply, Kiran Musunuru, Yoseph Barash, John A Germiller, Ian D Krantz, Russ P Carstens, Douglas J Epstein
Alternative splicing contributes to gene expression dynamics in many tissues, yet its role in auditory development remains unclear. We performed whole-exome sequencing in individuals with sensorineural hearing loss (SNHL) and identified pathogenic mutations in Epithelial Splicing-Regulatory Protein 1 (ESRP1). Patient-derived induced pluripotent stem cells showed alternative splicing defects that were restored upon repair of an ESRP1 mutant allele. To determine how ESRP1 mutations cause hearing loss, we evaluated Esrp1-/- mouse embryos and uncovered alterations in cochlear morphogenesis, auditory hair cell differentiation, and cell fate specification...
November 6, 2017: Developmental Cell
https://www.readbyqxmd.com/read/29026781/bulge-region-as-a-putative-hair-follicle-stem-cells-niche-a-brief-review
#13
REVIEW
Sanaz Joulai Veijouye, Abazar Yari, Fatemeh Heidari, Nayereh Sajedi, Fatemeh Ghoroghi Moghani, Maliheh Nobakht
BACKGROUND: Hair follicle stem cells exist in different sites. Most of the hair follicle stem cells are reside in niche called bulge. Bulge region is located between the opening of sebaceous gland and the attachment site of the arrector pili muscle. METHODS: Data were collected using databases and resources of PubMed, Web of Science, Science Direct, Scopus, MEDLINE and their references from the earliest available published to identify English observational studies on hair follicle bulge region...
September 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28973399/trichothiodystrophy-causative-tfiie%C3%AE-mutation-affects-transcription-in-highly-differentiated-tissue
#14
Arjan F Theil, Imke K Mandemaker, Emile van den Akker, Sigrid M A Swagemakers, Anja Raams, Tatjana Wüst, Jurgen A Marteijn, Jacques C Giltay, Richard M Colombijn, Ute Moog, Urania Kotzaeridou, Mehrnaz Ghazvini, Marieke von Lindern, Jan H J Hoeijmakers, Nicolaas G J Jaspers, Peter J van der Spek, Wim Vermeulen
The rare recessive developmental disorder Trichothiodystrophy (TTD) is characterized by brittle hair and nails. Patients also present a variable set of poorly explained additional clinical features, including ichthyosis, impaired intelligence, developmental delay and anemia. About half of TTD patients are photosensitive due to inherited defects in the DNA repair and transcription factor II H (TFIIH). The pathophysiological contributions of unrepaired DNA lesions and impaired transcription have not been dissected yet...
September 11, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28911317/generation-of-ips-derived-model-cells-for-analyses-of-hair-shaft-differentiation
#15
Takumi Kido, Tomoatsu Horigome, Minori Uda, Naoki Adachi, Yohei Hirai
Biological evaluation of hair growth/differentiation activity in vitro has been a formidable challenge, primarily due to the lack of relevant model cell systems. To solve this problem, we generated a stable model cell line in which successive differentiation via epidermal progenitors to hair components is easily inducible and traceable. Mouse induced pluripotent stem (iPS) cell-derived cells were selected to stably express a tetracycline (Tet)-inducible bone morphogenic protein-4 (BMP4) expression cassette and a luciferase reporter driven by a hair-specific keratin 31 gene (krt31) promoter (Tet-BMP4-KRT31-Luc iPS)...
September 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28886268/implanted-hair-follicle-associated-pluripotent-hap-stem-cells-encapsulated-in-polyvinylidene-fluoride-membrane-cylinders-promote-effective-recovery-of-peripheral-nerve-injury
#16
Aiko Yamazaki, Kohya Obara, Natsuko Tohgi, Kyoumi Shirai, Sumiyuki Mii, Yuko Hamada, Nobuko Arakawa, Ryoichi Aki, Robert M Hoffman, Yasuyuki Amoh
Hair follicle-associated-pluripotent (HAP) stem cells are located in the bulge area of the hair follicle, express the stem-cell marker, nestin, and have been shown to differentiate to nerve cells, glial cells, keratinocytes, smooth muscle cells, cardiac muscle cells, and melanocytes. Transplanted HAP stem cells promote the recovery of peripheral nerve and spinal cord injuries and have the potential for heart regeneration as well. In the present study, we implanted mouse green fluorescent protein (GFP)-expressing HAP stem-cell spheres encapsulated in polyvinylidene fluoride (PVDF)-membrane cylinders into the severed sciatic nerve of immunocompetent and immunocompromised (nude) mice...
October 18, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28749199/hair-follicle-associated-pluripotent-hap-stem-cells
#17
Yasuyuki Amoh, Robert M Hoffman
Various types of stem cells reside in the skin, including keratinocyte progenitor cells, melanocyte progenitor cells, skin-derived precursors (SKPs), and nestin-expressing hair follicle-associated-pluripotent (HAP) stem cells. HAP stem cells, located in the bulge area of the hair follicle, have been shown to differentiate to nerve cells, glial cells, keratinocytes, smooth muscle cells, cardiac muscle cells, and melanocytes. HAP stem cells are positive for the stem-cell marker CD34, as well as K15-negative, suggesting their relatively undifferentiated state...
July 27, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28686713/transcriptome-wide-comparison-of-the-impact-of-atoh1-and-mir-183-family-on-pluripotent-stem-cells-and-multipotent-otic-progenitor-cells
#18
COMPARATIVE STUDY
Michael Ebeid, Prashanth Sripal, Jason Pecka, Kirk W Beisel, Kelvin Kwan, Garrett A Soukup
Over 5% of the global population suffers from disabling hearing loss caused by multiple factors including aging, noise exposure, genetic predisposition, or use of ototoxic drugs. Sensorineural hearing loss is often caused by the loss of sensory hair cells (HCs) of the inner ear. A barrier to hearing restoration after HC loss is the limited ability of mammalian auditory HCs to spontaneously regenerate. Understanding the molecular mechanisms orchestrating HC development is expected to facilitate cell replacement therapies...
2017: PloS One
https://www.readbyqxmd.com/read/28643534/transplantation-of-mouse-induced-pluripotent-stem-cells-into-the-cochlea-for-the-treatment-of-sensorineural-hearing-loss
#19
Jing Chen, Lina Guan, Hengtao Zhu, Shan Xiong, Liang Zeng, Hongqun Jiang
CONCLUSION: Mouse-induced pluripotent stem cells (iPSCs) could differentiate into hair cell-like cells and spiral ganglion-like cells after transplantation into mouse cochleae, but it cannot improve the auditory brain response (ABR) thresholds in short term. OBJECTIVE: To evaluate the potential of iPSCs for use as a source of transplants for the treatment of sensorineural hearing loss (SNHL). METHODS: Establishing SNHL mice model, then injecting the iPSCs or equal volume DMEM basic medium into the cochleae, respectively...
June 23, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28602615/pten-mediates-activation-of-core-clock-protein-bmal1-and-accumulation-of-epidermal-stem-cells
#20
Chiara Zagni, Luciana O Almeida, Tarek Balan, Marco T Martins, Luciana K Rosselli-Murai, Petros Papagerakis, Rogerio M Castilho, Cristiane H Squarize
Tissue integrity requires constant maintenance of a quiescent, yet responsive, population of stem cells. In the skin, hair follicle stem cells (HFSCs) that reside within the bulge maintain tissue homeostasis in response to activating cues that occur with each new hair cycle or upon injury. We found that PTEN, a major regulator of the PI3K-AKT pathway, controlled HFSC number and size in the bulge and maintained genomically stable pluripotent cells. This regulatory function is central for HFSC quiescence, where PTEN-deficiency phenotype is in part regulated by BMAL1...
July 11, 2017: Stem Cell Reports
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