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https://www.readbyqxmd.com/read/27108086/multiplex-snapshot-a-new-simple-and-efficient-cyp2d6-and-adrb1-genotyping-method
#1
Songtao Ben, Rhonda M Cooper-DeHoff, Hanna K Flaten, Oghenero Evero, Tracey M Ferrara, Richard A Spritz, Andrew A Monte
BACKGROUND: Reliable, inexpensive, high-throughput genotyping methods are required for clinical trials. Traditional assays require numerous enzyme digestions or are too expensive for large sample volumes. Our objective was to develop an inexpensive, efficient, and reliable assay for CYP2D6 and ADRB1 accounting for numerous polymorphisms including gene duplications. MATERIALS AND METHODS: We utilized the multiplex SNaPshot® custom genotype method to genotype CYP2D6 and ADRB1...
2016: Human Genomics
https://www.readbyqxmd.com/read/26137835/conducting-the-student-interview-it-s-your-best-early-chance-to-spot-candidates-not-suited-for-ems
#2
Mike Rubin
Student interviews help ensure a match between classroom imperatives and candidates'capabilities. Exploring students'maturity and judgment is often more important than confirming their academic proficiency. Walking into an interview unprepared can be as damaging for interviewers as for interviewees. Selecting the right people and the right process for conducting interviews depends on planning and practice. A team approach to interviewing can be particularly effective if team members take the time to discuss topics and roles before meeting with candidates...
June 2015: EMS World
https://www.readbyqxmd.com/read/25734937/electrical-myostimulation-ems-improves-glucose-metabolism-and-oxygen-uptake-in-type-2-diabetes-mellitus-patients-results-from-the-ems-study
#3
Frank van Buuren, Dieter Horstkotte, Klaus Peter Mellwig, Andreas Fründ, Marios Vlachojannis, Nicola Bogunovic, Zisis Dimitriadis, Jürgen Vortherms, Reed Humphrey, Josef Niebauer
AIMS: In patients with type 2 diabetes mellitus (T2DM) exercise training is recommended to improve glycemic control. Electrical myostimulation (EMS) of skeletal muscles is a new method to increase exercise capacity in patients with chronic heart failure. The aim of this study was to investigate the effects of EMS in T2DM on glucose metabolism, body composition, and exercise performance using a newly designed stimulation suit that involves trunk, leg, and arm muscles. SUBJECTS AND METHODS: Fifteen individuals (nine males; 61...
June 2015: Diabetes Technology & Therapeutics
https://www.readbyqxmd.com/read/25329938/inferior-g-protection-with-an-electrical-muscle-stimulation-suit-compared-to-a-standard-g-suit
#4
COMPARATIVE STUDY
Ulf I Balldin, John A Gibbons
BACKGROUND: At +1 Gz, electrical muscle stimulation (EMS) has been shown to increase systemic blood pressure similarly to a standard G-suit or lower body muscle straining. It was hypothesized that EMS might improve G protection at increased G levels. METHODS: An EMS suit was developed with electrodes over the calves, thighs, gluteal, and abdominal muscles. Using nine subjects, the EMS suit was compared to a standard five-bladder G-suit during various G profiles up to +9 Gz in a human-rated centrifuge with EMS activated by electrical muscle stimulators at G levels at or above +4 Gz...
November 2014: Aviation, Space, and Environmental Medicine
https://www.readbyqxmd.com/read/25191748/emergency-medical-support-for-a-manned-stratospheric-balloon-test-program
#5
Rebecca S Blue, Sean C Norton, Jennifer Law, James M Pattarini, Erik L Antonsen, Alejandro Garbino, Jonathan B Clark, Matthew W Turney
INTRODUCTION: Red Bull Stratos was a commercial program that brought a test parachutist, protected by a full-pressure suit, in a stratospheric balloon with pressurized capsule to over 127,582 ft (38,969 m), from which he free fell and subsequently parachuted to the ground. Given that the major risks to the parachutist included ebullism, negative Gz (toe-to-head) acceleration exposure from an uncontrolled flat spin, and trauma, a comprehensive plan was developed to recover the parachutist under nominal conditions and to respond to any medical contingencies that might have arisen...
October 2014: Prehospital and Disaster Medicine
https://www.readbyqxmd.com/read/24957424/increased-mortality-associated-with-ems-transport-of-gunshot-wound-victims-when-compared-to-private-vehicle-transport
#6
COMPARATIVE STUDY
Syed Nabeel Zafar, Adil H Haider, Kent A Stevens, Nik Ray-Mazumder, Mehreen T Kisat, Eric B Schneider, Albert Chi, Samuel M Galvagno, Edward E Cornwell, David T Efron, Elliott R Haut
BACKGROUND: Recent studies suggest that mode of transport affects survival in penetrating trauma patients. We hypothesised that there is wide variation in transport mode for patients with gunshot wounds (GSW) and there may be a mortality difference for GSW patients transported by emergency medical services (EMS) vs. private vehicle (PV). STUDY DESIGN: We studied adult (≥16 years) GSW patients in the National Trauma Data Bank (2007-2010). Level 1 and 2 trauma centres (TC) receiving ≥50 GSW patients per year were included...
September 2014: Injury
https://www.readbyqxmd.com/read/24548058/degradation-of-benzodiazepines-after-120-days-of-ems-deployment
#7
COMPARATIVE STUDY
Jason T McMullan, Elizabeth Jones, Bruce Barnhart, Kurt Denninghoff, Daniel Spaite, Erin Zaleski, Robert Silbergleit
INTRODUCTION: EMS treatment of status epilepticus improves outcomes, but the benzodiazepine best suited for EMS use is unclear, given potential high environmental temperature exposures. OBJECTIVE: To describe the degradation of diazepam, lorazepam, and midazolam as a function of temperature exposure and time over 120 days of storage on active EMS units. METHODS: Study boxes containing vials of diazepam, lorazepam, and midazolam were distributed to 4 active EMS units in each of 2 EMS systems in the southwestern United States during May-August 2011...
July 2014: Prehospital Emergency Care
https://www.readbyqxmd.com/read/24301398/giving-ems-flexibility-in-transporting-low-acuity-patients-could-generate-substantial-medicare-savings
#8
Abby Alpert, Kristy G Morganti, Gregg S Margolis, Jeffrey Wasserman, Arthur L Kellermann
Some Medicare beneficiaries who place 911 calls to request an ambulance might safely be cared for in settings other than the emergency department (ED) at lower cost. Using 2005-09 Medicare claims data and a validated algorithm, we estimated that 12.9-16.2 percent of Medicare-covered 911 emergency medical services (EMS) transports involved conditions that were probably nonemergent or primary care treatable. Among beneficiaries not admitted to the hospital, about 34.5 percent had a low-acuity diagnosis that might have been managed outside the ED...
December 2013: Health Affairs
https://www.readbyqxmd.com/read/23579764/electrical-myostimulation-improves-left-ventricular-function-and-peak-oxygen-consumption-in-patients-with-chronic-heart-failure-results-from-the-exems-study-comparing-different-stimulation-strategies
#9
COMPARATIVE STUDY
Frank van Buuren, Klaus Peter Mellwig, Christian Prinz, Britta Körber, Andreas Fründ, Dirk Fritzsche, Lothar Faber, Tanja Kottmann, Nicola Bogunovic, Johannes Dahm, Dieter Horstkotte
AIMS: Electromyostimulation (EMS) of thigh and gluteal muscles is a strategy to increase exercise capacity in patients with chronic heart failure (CHF). The aim of this non-randomised pilot study was to investigate the effects of different stimulation strategies in CHF patients using a newly developed stimulation suit also involving trunk and arm muscles [extended electromyostimulation (exEMS)] in comparison with EMS therapy limited to gluteal and leg muscles (limEMS). METHODS: 60 individuals joined the EMS training programme...
July 2013: Clinical Research in Cardiology: Official Journal of the German Cardiac Society
https://www.readbyqxmd.com/read/22873666/in-vitro-assessment-of-a-new-portable-ballistic-lithotripter-with-percutaneous-and-ureteroscopic-models
#10
Agnes J Wang, Gregory T Baldwin, James C Gabriel, F Hadley Cocks, Zachariah G Goldsmith, Muhammad W Iqbal, Gaston M Astroza, W Neal Simmons, Pei Zhong, Glenn M Preminger, Michael Eric Lipkin
BACKGROUND AND PURPOSE: The EMS Swiss LithoBreaker is a new, portable, electrokinetic lithotripter. We compared its tip velocity and displacement characteristics with a handheld, pneumatic lithotripter LMA StoneBreaker.™ We also evaluated fragmentation efficiency using in vitro models of percutaneous and ureteroscopic stone fragmentation. MATERIALS AND METHODS: Displacement and velocity profiles were measured for 1-mm and 2-mm probes using a laser beam aimed at a photo detector...
November 2012: Journal of Endourology
https://www.readbyqxmd.com/read/22650429/does-spatial-variation-in-egg-thiamine-and-fatty-acid-concentration-of-lake-michigan-lake-trout-salvelinus-namaycush-lead-to-differential-early-mortality-syndrome-and-yolk-oedema-mortality-in-offspring
#11
S J Czesny, J Rinchard, B-J Lee, K Dabrowski, J M Dettmers, Y Cao
Individual variation in fatty-acid and thiamine concentrations were determined in lake trout Salvelinus namaycush eggs collected at two spawning grounds in Lake Michigan. A suite of predictor variables, including spawning location, egg fatty-acid and thiamine concentrations, were used to attempt to explain cause-and-effect in early life stage mortality among S. namaycush families. Lipid and fatty-acid composition of S. namaycush eggs differed between spawning locations. Salvelinus namaycush offspring from south-western Lake Michigan were affected by a high occurrence of yolk oedema, whereas a higher frequency of early mortality syndrome (EMS) was observed among offspring from the north-western part of the lake...
June 2012: Journal of Fish Biology
https://www.readbyqxmd.com/read/22152063/high-resolution-melt-hrm-analysis-is-an-efficient-tool-to-genotype-ems-mutants-in-complex-crop-genomes
#12
Seosamh O Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Ostergaard, Graham J King, Phillip J White, Martin R Broadley
BACKGROUND: Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generated in a number of crop species including Brassica rapa. Genetic analysis of mutants identified by TILLING requires an efficient, high-throughput and cost effective genotyping method to track the mutations through numerous generations. High resolution melt (HRM) analysis has been used in a number of systems to identify single nucleotide polymorphisms (SNPs) and insertion/deletions (IN/DELs) enabling the genotyping of different types of samples...
December 8, 2011: Plant Methods
https://www.readbyqxmd.com/read/21880107/a-combined-biochemical-screen-and-tilling-approach-identifies-mutations-in-sorghum-bicolor-l-moench-resulting-in-acyanogenic-forage-production
#13
Cecilia K Blomstedt, Roslyn M Gleadow, Natalie O'Donnell, Peter Naur, Kenneth Jensen, Tomas Laursen, Carl Erik Olsen, Peter Stuart, John D Hamill, Birger Lindberg Møller, Alan D Neale
Cyanogenic glucosides are present in several crop plants and can pose a significant problem for human and animal consumption, because of their ability to release toxic hydrogen cyanide. Sorghum bicolor L. contains the cyanogenic glucoside dhurrin. A qualitative biochemical screen of the M2 population derived from EMS treatment of sorghum seeds, followed by the reverse genetic technique of Targeted Induced Local Lesions in Genomes (TILLING), was employed to identify mutants with altered hydrogen cyanide potential (HCNp)...
January 2012: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/21830918/paramedic-contact-to-balloon-in-less-than-90-minutes-a-successful-strategy-for-st-segment-elevation-myocardial-infarction-bypass-to-primary-percutaneous-coronary-intervention-in-a-canadian-emergency-medical-system
#14
Sheldon Cheskes, Linda Turner, Ruth Foggett, Maud Huiskamp, Dean Popov, Sue Thomson, Greg Sage, Randy Watson, Richard Verbeek
INTRODUCTION: Few systems worldwide have achieved the benchmark time of less than 90 minutes from emergency medical services (EMS) contact to balloon inflation (E2B) for patients sustaining ST-segment elevation myocardial infarction (STEMI). We describe a successful EMS systems approach using a combination of paramedic and 12-lead electrocardiogram (ECG) software interpretation to activate a STEMI bypass protocol. OBJECTIVES: To determine the proportion of patients who met the benchmark of E2B in less than 90 minutes after institution of a regional paramedic activated STEMI bypass to primary PCI protocol...
October 2011: Prehospital Emergency Care
https://www.readbyqxmd.com/read/20536616/stroke-education-discrepancies-among-factors-influencing-prehospital-delay-and-stroke-knowledge
#15
REVIEW
Yvonne Teuschl, Michael Brainin
BACKGROUND: Time is essential for the treatment of acute stroke. Much time is lost outside the hospital, either due to failure in identifying stroke symptoms or due to a delay in notification or transport. We review studies reporting factors associated with better stroke knowledge and shorter time delays. We summarise the evidences for the effect of stroke knowledge and education on people's reaction in the acute situation of stroke. METHODS: We searched MEDLINE for studies reporting factors associated with prehospital time of stroke patients, or knowledge of stroke symptoms...
June 2010: International Journal of Stroke: Official Journal of the International Stroke Society
https://www.readbyqxmd.com/read/20380715/a-rich-tilling-resource-for-studying-gene-function-in-brassica-rapa
#16
Pauline Stephenson, David Baker, Thomas Girin, Amandine Perez, Stephen Amoah, Graham J King, Lars Østergaard
BACKGROUND: The Brassicaceae family includes the model plant Arabidopsis thaliana as well as a number of agronomically important species such as oilseed crops (in particular Brassica napus, B. juncea and B. rapa) and vegetables (eg. B. rapa and B. oleracea). Separated by only 10-20 million years, Brassica species and Arabidopsis thaliana are closely related, and it is expected that knowledge obtained relating to Arabidopsis growth and development can be translated into Brassicas for crop improvement...
April 9, 2010: BMC Plant Biology
https://www.readbyqxmd.com/read/20148411/prediction-of-metabolic-function-from-limited-data-lumped-hybrid-cybernetic-modeling-l-hcm
#17
Hyun-Seob Song, Doraiswami Ramkrishna
Motivated by the need for a quick quantitative assessment of metabolic function without extensive data, we present an adaptation of the cybernetic framework, denoted as the lumped hybrid cybernetic model (L-HCM), which combines the attributes of the classical lumped cybernetic model (LCM) and the recently developed HCM. The basic tenet of L-HCM and HCM is the same, that is, they both view the uptake flux as being split among diverse pathways in an optimal way as a result of cellular regulation such that some chosen metabolic objective is realized...
June 1, 2010: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/18979248/ultrastructural-imaging-of-cell-fusion-in-caenorhabditis-elegans
#18
Star Ems, William A Mohler
Caenorhabditis elegans is a well-established model system particularly suited for studying cell-cell fusion because of its highly predictable and rapid development and its known cell lineage. This chapter focuses on understanding the ultrastructural components of cell fusion through the use of transmission electron microscopy (TEM). Published TEM studies have described the initial demonstration of syncytial cells in the worm, the vesiculation of the bilayers between cells during widening of the normal fusion aperture, and the appearance of microfusion intermediates in the membranes of cells with fusion-defective mutations...
2008: Methods in Molecular Biology
https://www.readbyqxmd.com/read/18785362/an-electrical-muscle-stimulation-suit-for-increasing-blood-pressure
#19
Ulf Balldin, Lance Annicelli, John Gibbons, James Kisner
BACKGROUND: Electrical muscle stimulation (EMS) is used to strengthen muscles in rehabilitation of patients and for training of athletes. Voluntary muscle straining and an inflated anti-G suit increase the arterial blood pressure (BP) and give a pilot G protection during increased +Gz. This study's aim was to measure whether BP also increases with EMS of lower body muscles. METHODS: A suit with new cloth electrodes sewn into the garment was developed. There were 12 subjects who were tested in sitting position during 3 conditions with 10 consecutive periods of EMS, inflated anti-G suit (GS), or lower body muscle anti-G straining maneuvers (AGSM)...
September 2008: Aviation, Space, and Environmental Medicine
https://www.readbyqxmd.com/read/18416441/rapid-screening-and-characterization-of-drug-metabolites-using-a-multiple-ion-monitoring-dependent-ms-ms-acquisition-method-on-a-hybrid-triple-quadrupole-linear-ion-trap-mass-spectrometer
#20
Ming Yao, Li Ma, W Griffith Humphreys, Mingshe Zhu
A novel LC/MS/MS method that uses multiple ion monitoring (MIM) as a survey scan to trigger the acquisition of enhanced product ions (EPI) on a hybrid quadrupole-linear ion trap mass spectrometer (Q TRAP) was developed for drug metabolite identification. In the MIM experiment, multiple predicted metabolite ions were monitored in both Q1 and Q3. The collision energy in Q2 was set to a low value to minimize fragmentation. Results from analyzing ritonavir metabolites in rat hepatocytes demonstrate that MIM-EPI was capable of targeting a larger number of metabolites regardless of their fragmentation and retained sensitivity and duty cycle similar to multiple reaction monitoring (MRM)-EPI...
October 2008: Journal of Mass Spectrometry: JMS
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