Shahriar Koochekpour, Yu-Jun Zhuang, Rameen Beroukhim, Chia-Ling Hsieh, Matthias D Hofer, Haiyen E Zhau, Masao Hiraiwa, Daniel Y Pattan, Joy L Ware, Ronald B Luftig, Konrad Sandhoff, Charles L Sawyers, Kenneth J Pienta, Mark A Rubin, Robert L Vessella, William R Sellers, Oliver Sartor
We identified prosaposin (PSAP) as a secreted protein expressed in androgen-independent (AI) prostate cancer cells by cloning/sequencing, after probing a PC-3 cDNA library expressed in the lambdaTriplEx phagemid expression vector with a polyclonal rabbit antibody generated against pooled human seminal plasma. PSAP is a neurotrophic molecule; its deficiency or inactivation has proved to be lethal in man and mice, and in mice, it leads to abnormal development and atrophy of the prostate gland, despite normal testosterone levels...
December 2005: Genes, Chromosomes & Cancer