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https://www.readbyqxmd.com/read/27873249/application-of-a-bacillus-subtilis-whole-cell-biosensor-pliai-lux-for-the-identification-of-cell-wall-active-antibacterial-compounds
#1
Carolin Martina Kobras, Thorsten Mascher, Susanne Gebhard
Whole-cell biosensors, based on the visualization of a reporter strain's response to a particular stimulus, are a robust and cost-effective means to monitor defined environmental conditions or the presence of chemical compounds. One specific field in which such biosensors are frequently applied is drug discovery, i.e., the screening of large numbers of bacterial or fungal strains for the production of antimicrobial compounds. We here describe the application of a luminescence-based Bacillus subtilis biosensor for the discovery of cell wall active substances...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27865622/protecting-from-envelope-stress-variations-on-the-phage-shock-protein-theme
#2
REVIEW
Riccardo Manganelli, Maria Laura Gennaro
During envelope stress, critical inner-membrane functions are preserved by the phage-shock-protein (Psp) system, a stress response that emerged from work with Escherichia coli and other Gram-negative bacteria. Reciprocal regulatory interactions and multiple effector functions are well documented in these organisms. Searches for the Psp system across phyla reveals conservation of only one protein, PspA. However, examination of Firmicutes and Actinobacteria reveals that PspA orthologs associate with non-orthologous regulatory and effector proteins retaining functions similar to those in Gram-negative counterparts...
November 16, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27862269/arp2-3-complex-and-mps3-are-required-for-regulation-of-ribosome-biosynthesis-in-the-secretory-stress-response
#3
Yukari Yabuki, Masako Katayama, Yushi Kodama, Akiko Sakamoto, Ayumi Yatsuhashi, Kouichi Funato, Keiko Mizuta
Secretory defects cause transcriptional repression of ribosome biogenesis in Saccharomyces cerevisiae. However, the molecular mechanism underlying secretory defect-induced transcriptional repression of ribosome biogenesis remains to be fully elucidated. In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1-228 and cmd1-239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, fail to interact with Arc35, a component of the Arp2/3 complex...
November 10, 2016: Yeast
https://www.readbyqxmd.com/read/27829584/fe-3-dependent-epistasis-between-the-cpxr-activated-loci-and-the-pmra-activated-lps-modification-loci-in-salmonella-enterica
#4
Akinori Kato, Nami Higashino, Ryutaro Utsumi
Bacteria utilize varying combinations of two-component regulatory systems, many of which respond and adapt closely to stress conditions, thus expanding their niche steadily. While mechanisms of recognition and avoidance of the specific Fe(3+) signal by the PmrA/PmrB system is well understood, those of the CpxR/CpxA system are more complex because they can be induced by various stress conditions, which, in turn, expresses a variety of phenotypes. Here, we highlight another aspect of the CpxR/CpxA system; mutations in degP and yqjA genes, which are under the control of the system, exhibit an iron sensitive phenotype in the mutant background defective in the PmrA-dependent gene products that alter the pyrophosphate status of the lipid A moiety of lipopolysaccharide in Salmonella enterica...
November 8, 2016: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/27821607/novel-rpos-dependent-mechanisms-strengthen-the-envelope-permeability-barrier-during-stationary-phase
#5
Angela M Mitchell, Wei Wang, Thomas J Silhavy
: Gram-negative bacteria have effective methods of excluding toxic compounds including a largely impermeable outer membrane (OM) and a range of efflux pumps. Furthermore, when cells become nutrient-limited, RpoS enacts a global expression change providing cross-protection against many stresses. Here, we utilized sensitivity to an anionic detergent (sodium dodecyl sulfate, SDS) to model probe changes occurring to the cell's permeability barrier during nutrient limitation. Escherichia coli is resistant to SDS whether cells are actively growing, carbon-limited, or nitrogen-limited...
November 7, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27816520/inherently-variable-responses-to-glucocorticoid-stress-among-endogenous-retroviruses-isolated-from-23-mouse-strains
#6
Karen Hsu, Young-Kwan Lee, Alex Chew, Sophia Chiu, Debora Lim, David G Greenhalgh, Kiho Cho
Active participation of endogenous retroviruses (ERVs) in disease processes has been exemplified by the finding that the HERV (human ERV)-W envelope protein is involved in the pathogenesis of multiple sclerosis, an autoimmune disease. We also demonstrated that injury-elicited stressors alter the expression of murine ERVs (MuERVs), both murine leukemia virus-type and mouse mammary tumor virus (MMTV)-type (MMTV-MuERV). In this study, to evaluate MMTV-MuERVs' responses to stress (e.g., injury, infection)-elicited systemic glucocorticoid (GC) levels, we examined the GC-stress response of 64 MMTV-MuERV promoters isolated from the genomes of 23 mouse strains...
November 3, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27796471/deletion-of-degq-gene-enhances-outer-membrane-vesicle-production-of-shewanella-oneidensis-cells
#7
Yoshihiro Ojima, Thivagaran Mohanadas, Kosei Kitamura, Shota Nunogami, Reiki Yajima, Masahito Taya
Shewanella oneidensis is a Gram-negative facultative anaerobe that can use a wide variety of terminal electron acceptors for anaerobic respiration. In this study, S. oneidensis degQ gene, encoding a putative periplasmic serine protease, was cloned and expressed. The activity of purified DegQ was inhibited by diisopropyl fluorophosphate, a typical serine protease-specific inhibitor, indicating that DegQ is a serine protease. In-frame deletion and subsequent complementation of the degQ were carried out to examine the effect of envelope stress on the production of outer membrane vesicles (OMVs)...
October 31, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27790614/antimicrobial-activity-of-cationic-antimicrobial-peptides-against-gram-positives-current-progress-made-in-understanding-the-mode-of-action-and-the-response-of-bacteria
#8
REVIEW
Soraya Omardien, Stanley Brul, Sebastian A J Zaat
Antimicrobial peptides (AMPs) have been proposed as a novel class of antimicrobials that could aid the fight against antibiotic resistant bacteria. The mode of action of AMPs as acting on the bacterial cytoplasmic membrane has often been presented as an enigma and there are doubts whether the membrane is the sole target of AMPs. Progress has been made in clarifying the possible targets of these peptides, which is reported in this review with as focus gram-positive vegetative cells and spores. Numerical estimates are discussed to evaluate the possibility that targets, other than the membrane, could play a role in susceptibility to AMPs...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27784136/the-role-of-apolipoprotein-n-acyl-transferase-lnt-in-the-lipidation-of-factor-h-binding-protein-of-neisseria-meningitidis-strain-mc58-and-its-potential-as-a-drug-target
#9
Rag da Silva, C P Churchward, A V Karlyshev, O Eleftheriadou, A K Snabaitis, M R Longman, A Ryan, R Griffin
BACKGROUND AND PURPOSE: The level of cell surface expression of the meningococcal vaccine antigen, Factor H binding protein (FHbp) varies between and within strains and this limits the breadth of strains that can be targeted by FHbp-based vaccines. The molecular pathway dictating expression of FHbp at the cell surface, including its lipidation, sorting to the outer membrane and export, and the potential regulation of this pathway have not been investigated until now. This knowledge will aid our evaluation of FHbp vaccines...
October 26, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27766354/global-transcriptomic-analysis-of-the-response-of-corynebacterium-glutamicum-to-ferulic-acid
#10
Can Chen, Junfeng Pan, Xiaobing Yang, He Xiao, Yaoling Zhang, Meiru Si, Xihui Shen, Yao Wang
Corynebacterium glutamicum can survive by using ferulic acid as the sole carbon source. In this study, we assessed the response of C. glutamicum to ferulic acid stress by means of a global transcriptional response analysis. The transcriptional data showed that several genes involved in degradation of ferulic acid were affected. Moreover, several genes related to the stress response; protein protection or degradation and DNA repair; replication, transcription and translation; and the cell envelope were differentially expressed...
October 20, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27760214/global-transcriptomic-analysis-of-the-response-of-corynebacterium-glutamicum-to-vanillin
#11
Can Chen, Junfeng Pan, Xiaobing Yang, Chenghao Guo, Wei Ding, Meiru Si, Yi Zhang, Xihui Shen, Yao Wang
Lignocellulosic biomass is an abundant and renewable resource for biofuels and bio-based chemicals. Vanillin is one of the major phenolic inhibitors in biomass production using lignocellulose. To assess the response of Corynebacterium glutamicum to vanillin stress, we performed a global transcriptional response analysis. The transcriptional data showed that the vanillin stress not only affected the genes involved in degradation of vanillin, but also differentially regulated several genes related to the stress response, ribosome/translation, protein secretion, and the cell envelope...
2016: PloS One
https://www.readbyqxmd.com/read/27746771/transcriptomic-and-proteomic-analysis-of-oenococcus-oeni-adaptation-to-wine-stress-conditions
#12
Mar Margalef-Català, Isabel Araque, Albert Bordons, Cristina Reguant, Joaquín Bautista-Gallego
Oenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF start have been studied. DNA microarrays were used for the transcriptomic analysis and two complementary proteomic techniques, 2-D DIGE and iTRAQ labeling were used to analyze the proteomic response. One of the most influenced functions in PSU-1 due to inoculation into wine-like medium (WLM) was translation, showing the over-expression of certain ribosomal genes and the corresponding proteins...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27736975/cpxr-activates-mexab-oprm-efflux-pump-expression-and-enhances-antibiotic-resistance-in-both-laboratory-and-clinical-nalb-type-isolates-of-pseudomonas-aeruginosa
#13
Zhe-Xian Tian, Xue-Xian Yi, Anna Cho, Fergal O'Gara, Yi-Ping Wang
Resistance-Nodulation-Division (RND) efflux pumps are responsible for multidrug resistance in Pseudomonas aeruginosa. In this study, we demonstrate that CpxR, previously identified as a regulator of the cell envelope stress response in Escherichia coli, is directly involved in activation of expression of RND efflux pump MexAB-OprM in P. aeruginosa. A conserved CpxR binding site was identified upstream of the mexA promoter in all genome-sequenced P. aeruginosa strains. CpxR is required to enhance mexAB-oprM expression and drug resistance, in the absence of repressor MexR, in P...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27720009/the-journey-of-lipoproteins-through-the-cell-one-birthplace-multiple-destinations
#14
J Szewczyk, J-F Collet
Bacterial lipoproteins are a very diverse group of proteins characterized by the presence of an N-terminal lipid moiety that serves as a membrane anchor. Lipoproteins have a wide variety of crucial functions, ranging from envelope biogenesis to stress response. In Gram-negative bacteria, lipoproteins can be targeted to various destinations in the cell, including the periplasmic side of the cytoplasmic or outer membrane, the cell surface or the external milieu. The sorting mechanisms have been studied in detail in Escherichia coli, but exceptions to the rules established in this model bacterium exist in other bacteria...
2016: Advances in Microbial Physiology
https://www.readbyqxmd.com/read/27698088/interactions-between-the-cytoplasmic-domains-of-pspb-and-pspc-silence-the-yersinia-enterocolitica-phage-shock-protein-response
#15
Josué Flores-Kim, Andrew J Darwin
: The Phage shock protein (Psp) system is a widely conserved cell envelope stress response that is essential for the virulence of some bacteria, including Yersinia enterocolitica Recruitment of PspA by the inner membrane PspB•PspC complex characterizes the activated state of this response. The PspB•PspC complex has been proposed to be a stress-responsive switch, changing from an OFF to an ON state in response to an inducing stimulus. In the OFF state, PspA cannot access its binding site in the C-terminal cytoplasmic domain of PspC (PspC(CT)) because this site is bound to PspB...
October 3, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27685640/the-rna-chaperone-hfq-is-involved-in-colony-morphology-nutrient-utilization-and-oxidative-and-envelope-stress-response-in-vibrio-alginolyticus
#16
Yiqin Deng, Chang Chen, Zhe Zhao, Jingjing Zhao, Annick Jacq, Xiaochun Huang, Yiying Yang
Hfq is a global regulator that is involved in environmental adaptation of bacteria and in pathogenicity. To gain insight into the role of Hfq in Vibrio alginolyticus, an hfq deletion mutant was constructed in V. alginolyticus ZJ-T strain and phenotypically characterized. Deletion of hfq led to an alteration of colony morphology and reduced extracellular polysaccharide production, a general impairment of growth in both rich medium and minimal media with different carbon sources or amino acids, enhanced sensitivity to oxidative stress and to several antibiotics...
2016: PloS One
https://www.readbyqxmd.com/read/27681379/new-insights-into-the-mechanisms-of-acetic-acid-resistance-in-acetobacter-pasteurianus-using-itraq-dependent-quantitative-proteomic-analysis
#17
Kai Xia, Ning Zang, Junmei Zhang, Hong Zhang, Yudong Li, Ye Liu, Wei Feng, Xinle Liang
Acetobacter pasteurianus is the main starter in rice vinegar manufacturing due to its remarkable abilities to resist and produce acetic acid. Although several mechanisms of acetic acid resistance have been proposed and only a few effector proteins have been identified, a comprehensive depiction of the biological processes involved in acetic acid resistance is needed. In this study, iTRAQ-based quantitative proteomic analysis was adopted to investigate the whole proteome of different acidic titers (3.6, 7.1 and 9...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27645386/transcriptional-responses-of-escherichia-coli-to-a-small-molecule-inhibitor-of-lolcde-an-essential-component-of-the-lipoprotein-transport-pathway
#18
Christian Lorenz, Thomas J Dougherty, Stephen Lory
: In Gram-negative bacteria, a dedicated machinery consisting of LolABCDE components targets lipoproteins to the outer membrane. We used a previously identified small-molecule inhibitor of the LolCDE complex of Escherichia coli to assess the global transcriptional consequences of interference with lipoprotein transport. Exposure of E. coli to the LolCDE inhibitor at concentrations leading to minimal and significant growth inhibition, followed by transcriptome sequencing, identified a small group of genes whose transcript levels were decreased and a larger group whose mRNA levels increased 10- to 100-fold compared to those of untreated cells...
December 1, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27631095/ultrastructural-histometric-evidence-for-expansion-of-the-sustentacular-cell-envelope-in-response-to-hypersecretion-of-adrenal-chromaffin-cells-in-mice
#19
Sawetree Pakkarato, Wipawee Thoungseabyoun, Apussara Tachow, Atsara Rawangwong, Yoshiteru Kagawa, Yuji Owada, Hisatake Kondo, Wiphawi Hipkaeo
In our previous immuno-light microscopic study with an antibody for fatty acid binding protein of type 7 or brain type (FABP-7, B-FABP), the adrenomedullary sustentacular cells were revealed to have secondary processes that present faint immunostaining and an ill-defined sheet-like appearance, in addition to the well-recognized primary processes that present distinct immunostaining and a fibrous appearance. The secondary processes were regarded as corresponding to known ultrastructural profiles of sustentacular cells with a thickness of less than 0...
September 15, 2016: Anatomical Science International
https://www.readbyqxmd.com/read/27620730/adaptive-response-of-rhodococcus-opacus-pwd4-to-salt-and-phenolic-stress-on-the-level-of-mycolic-acids
#20
Carla C C R de Carvalho, Martin A Fischer, Sandra Kirsten, Birgit Würz, Lukas Y Wick, Hermann J Heipieper
Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, the mycolic acids. In order to investigate the adaptive changes in the mycolic acid composition, we investigated the composition of mycolic acids during the response both to osmotic stress caused by NaCl and to 4-chlorophenol in Rhodococcus opacus PWD4...
December 2016: AMB Express
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