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stress response membrane bacteria

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https://www.readbyqxmd.com/read/28346358/genomic-evidence-for-bacterial-determinants-influencing-obesity-development
#1
Raphael D Isokpehi, Shaneka S Simmons, Matilda O Johnson, Marinelle Payton
Obesity is a major global public health problem requiring multifaceted interventional approaches including dietary interventions with probiotic bacteria. High-throughput genome sequencing of microbial communities in the mammalian gastrointestinal system continues to present diverse protein function information to understand the bacterial determinants that influence obesity development. The goal of the research reported in this article was to identify biological processes in probiotic bacteria that could influence the mechanisms for the extraction of energy from diet in the human gastrointestinal system...
March 26, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28300180/systems-level-understanding-of-ethanol-induced-stresses-and-adaptation-in-e-coli
#2
Huansheng Cao, Du Wei, Yuedong Yang, Yu Shang, Gaoyang Li, Yaoqi Zhou, Qin Ma, Ying Xu
Understanding ethanol-induced stresses and responses in biofuel-producing bacteria at systems level has significant implications in engineering more efficient biofuel producers. We present a computational study of transcriptomic and genomic data of both ethanol-stressed and ethanol-adapted E. coli cells with computationally predicated ethanol-binding proteins and experimentally identified ethanol tolerance genes. Our analysis suggests: (1) ethanol damages cell wall and membrane integrity, causing increased stresses, particularly reactive oxygen species, which damages DNA and reduces the O2 level; (2) decreased cross-membrane proton gradient from membrane damage, coupled with hypoxia, leads to reduced ATP production by aerobic respiration, driving cells to rely more on fatty acid oxidation, anaerobic respiration and fermentation for ATP production; (3) the reduced ATP generation results in substantially decreased synthesis of macromolecules; (4) ethanol can directly bind 213 proteins including transcription factors, altering their functions; (5) all these changes together induce multiple stress responses, reduced biosynthesis, cell viability and growth; and (6) ethanol-adapted E...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299749/osmotic-stress-in-colony-and-planktonic-cells-of-pseudomonas-putida-mt-2-revealed-significant-differences-in-adaptive-response-mechanisms
#3
Nancy Hachicho, Astrid Birnbaum, Hermann J Heipieper
Planktonic cells and those grown on surfaces (or as colony biofilm) are known to show significant differences regarding growth behavior, cell physiology, gene expression and stress tolerance. In order to compare stress behavior of different growth forms, shake cultures for planktonic growth and agar plate cultivation for colony growth, were carried out with the well investigated model organism, Pseudomonas putida mt-2. Cells were exposed to sodium chloride to cause osmotic stress as one main environmental stressor bacteria have to cope with when growing in soil...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28285661/colonization-and-beneficial-effects-on-annual-ryegrass-by-mixed-inoculation-with-plant-growth-promoting-bacteria
#4
Nádia L Castanheira, Ana Catarina Dourado, Isabel Pais, José Semedo, Paula Scotti-Campos, Nuno Borges, Gilda Carvalho, Maria Teresa Barreto Crespo, Paula Fareleira
Multi-strain inoculants have increased potential to accomplish a diversity of plant needs, mainly attributed to its multi-functionality. This work evaluated the ability of a mixture of three bacteria to colonize and induce a beneficial response on the pasture crop annual ryegrass. Pseudomonas G1Dc10 and Paenibacillus G3Ac9 were previously isolated from annual ryegrass and were selected for their ability to perform multiple functions related to plant growth promotion. Sphingomonas azotifigens DSMZ 18530(T) was included due to nitrogen fixing ability...
May 2017: Microbiological Research
https://www.readbyqxmd.com/read/28277971/distinct-light-stress-and-nutrient-dependent-regulation-of-multiple-tryptophan-rich-sensory-protein-tspo-genes-in-the-cyanobacterium-fremyella-diplosiphon
#5
Andrea W U Busch, Beronda L Montgomery
The cyanobacterium Fremyella diplosiphon possesses 3 genes encoding homologs of the tryptophan-rich sensory protein (TSPO). TSPO proteins are membrane proteins implicated in stress responses across a range of organisms from bacteria to humans. Diverse TSPO proteins appear to generally bind tetrapyrrole ligands. Previously, we reported that one of these homologs, FdTSPO1, is involved in salt-, osmotic- and oxidative stress responses in F. diplosiphon. Here, we show distinct regulation of cellular mRNA levels of all 3 FdTSPO homologs by different abiotic stresses...
March 4, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28254301/in-vitro-cytotoxicity-effect-and-antibacterial-performance-of-human-lung-epithelial-cells-a549-activity-of-zinc-oxide-doped-tio2-nanocrystals-investigation-of-bio-medical-application-by-chemical-method
#6
K Kaviyarasu, N Geetha, K Kanimozhi, C Maria Magdalane, S Sivaranjani, A Ayeshamariam, J Kennedy, M Maaza
We report the synthesis of high quality ZnO doped TiO2 nanocrystals by chemical method at room temperature (RT), it can cause serious oxidative stress and DNA damage to human lung epithelial cells (A549) lines. Our aim in this study, to reduce the cytotoxicity effect of ZnO doped TiO2 nanocrystals are widely in biological fields. Several studies have been performed to understand the influence of ZnO doped titanium dioxide (TiO2-NPs) on cell function; however the effects of nanoparticle against to exposure on the cell membrane have been duly addressed fascinatingly so far...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28242719/tail-anchored-inner-membrane-protein-elab-increases-resistance-to-stress-while-reducing-persistence-in-escherichia-coli
#7
Yunxue Guo, Xiaoxiao Liu, Baiyuan Li, Jianyun Yao, Thomas K Wood, Xiaoxue Wang
Host-associated bacteria, such as Escherichia coli, often encounter various host-related stresses such as nutritional deprivation, oxidative stress and temperature shifts. There is growing interest in searching for small endogenous proteins that mediate stress responses. Here, we characterized a small C-tail anchored inner membrane protein ElaB in E. coli ElaB belongs to a class of tail-anchored inner membrane proteins with a C-terminal transmembrane domain but lacking an N-terminal signal sequence for membrane targeting...
February 27, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28242693/manganese-scavenging-and-oxidative-stress-response-mediated-by-type-vi-secretion-system-in-burkholderia-thailandensis
#8
Meiru Si, Chao Zhao, Brianne Burkinshaw, Bing Zhang, Dawei Wei, Yao Wang, Tao G Dong, Xihui Shen
Type VI secretion system (T6SS) is a versatile protein export machinery widely distributed in Gram-negative bacteria. Known to translocate protein substrates to eukaryotic and prokaryotic target cells to cause cellular damage, the T6SS has been primarily recognized as a contact-dependent bacterial weapon for microbe-host and microbial interspecies competition. Here we report contact-independent functions of the T6SS for metal acquisition, bacteria competition, and resistance to oxidative stress. We demonstrate that the T6SS-4 in Burkholderia thailandensis is critical for survival under oxidative stress and is regulated by OxyR, a conserved oxidative stress regulator...
February 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28217741/transcriptome-level-signatures-in-gene-expression-and-gene-expression-variability-during-bacterial-adaptive-evolution
#9
Keesha E Erickson, Peter B Otoupal, Anushree Chatterjee
Antibiotic-resistant bacteria are an increasingly serious public health concern, as strains emerge that demonstrate resistance to almost all available treatments. One factor that contributes to the crisis is the adaptive ability of bacteria, which exhibit remarkable phenotypic and gene expression heterogeneity in order to gain a survival advantage in damaging environments. This high degree of variability in gene expression across biological populations makes it a challenging task to identify key regulators of bacterial adaptation...
January 2017: MSphere
https://www.readbyqxmd.com/read/28191904/n-terminomics-identifies-prli42-as-a-membrane-miniprotein-conserved-in-firmicutes-and-critical-for-stressosome-activation-in-listeria-monocytogenes
#10
Francis Impens, Nathalie Rolhion, Lilliana Radoshevich, Christophe Bécavin, Mélodie Duval, Jeffrey Mellin, Francisco García Del Portillo, M Graciela Pucciarelli, Allison H Williams, Pascale Cossart
To adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. In Gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma B regulon. The stressosome is structurally well-characterized in Bacillus, but how it senses stress remains elusive. Here, we report a genome-wide N-terminomic approach in Listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniproteins, among which one, Prli42, is conserved in Firmicutes...
February 13, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28177291/expression-of-flagellin-and-key-regulatory-flagellar-genes-in-the-non-motile-bacterium-piscirickettsia-salmonis
#11
Gabriela P Carril, Fernando A Gómez, Sergio H Marshall
The Piscirickettsia salmonis genome was screened to evaluate potential flagella-related open reading frames, as well as their genomic organization and eventual expression. A complete and organized set of flagellar genes was found for P. salmonis, although no structural flagellum has ever been reported for this bacterium. To gain further understanding, the hierarchical flagellar cascade described for Legionella pneumophila was used as a reference model for putative analysis in P. salmonis. Specifically, 5 of the most relevant genes from this cascade were chosen, including 3 regulatory genes (fleQ, triggers the cascade; fliA, regulates the σ28-coding gene; and rpoN, an RNA polymerase-dependent gene) and 2 terminal structural genes (flaA and flaB, flagellin and a flagellin-like protein, respectively)...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28177232/adaptation-to-biocides-cetrimide-and-chlorhexidine-in-bacteria-from-organic-foods-association-with-tolerance-to-other-antimicrobials-and-physical-stresses
#12
Rebeca Gadea, Nicolás Glibota, Rubén Pérez Pulido, Antonio Gálvez, Elena Ortega
Chlorhexidine (CH) and quaternary ammonium compounds (QAC), such as cetrimide (CE), are widely used as disinfectants because of their broad antimicrobial spectrum. However, their frequent use for disinfection in different settings may promote bacterial drug resistance against both biocides and clinically relevant antibiotics. This study analyzes the effects of stepwise exposure to cetrimide (CE) and chlorhexidine (CH) of bacteria from organic foods and previously classified as biocide-sensitive. Gradual exposure of these strains to biocides resulted in mainly transient decreased antimicrobial susceptibility to other antibiotics and to biocides...
February 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28141494/butanol-is-cytotoxic-to-lactococcus-lactis-while-ethanol-and-hexanol-are-cytostatic
#13
Ann-Mette Meisner Hviid, Peter Rudahl Jensen, Mogens Kilstrup
Lactic acid bacteria currently used extensively by the dairy industry have a superior tolerance towards small chain alcohols, which makes them interesting targets for use in future bio-refineries. The mechanism underlying the alcohol tolerance of lactic acid bacteria has so far received little attention. In the present study the physiological alcohol stress response of Lactococcus lactis subsp. cremoris MG1363 towards the primary, even-chain alcohols; ethanol, butanol, and hexanol was characterized. The alcohol tolerance of L...
January 28, 2017: Microbiology
https://www.readbyqxmd.com/read/28131377/identification-of-otpr-regulated-srnas-in-brucella-melitensis-expressed-under-acidic-stress-and-their-roles-in-pathogenesis-and-metabolism
#14
Udayakumar S Vishnu, Jagadesan Sankarasubramanian, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Small RNAs (sRNAs) are the small regulatory molecules that regulate various biological processes in bacteria. Though the functions of sRNAs are well documented, very little information is available on the sRNAs of Brucella spp. The otpR is the response regulator of a two-component regulatory system, which plays a significant role in Brucella virulence. In this study, we identified the sRNAs expressed in B. melitensis 16M and its otpR mutant under acidic stress from the RNAseq dataset. We identified 94 trans-encoded and 948 cis-encoded sRNAs in B...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28115509/the-tat-substrate-sufi-is-critical-for-the-ability-of-yersinia-pseudotuberculosis-to-cause-systemic-infection
#15
Ummehan Avican, Tugrul Doruk, Yngve Östberg, Anna Fahlgren, Åke Forsberg
The Twin-arginine translocation (Tat) system targets folded proteins across the inner membrane and it is crucial for virulence in many important human pathogenic bacteria. Tat has been shown to be required for virulence of Yersinia pseudotuberculosis and we recently showed that the system is critical for different virulence related stress responses as well as for iron uptake. In this study, we wanted to address the role of the Tat substrates in in vivo virulence. Therefore, 22 genes encoding potential Tat substrates were mutated and each mutant was evaluated in competitive oral infection of mice...
January 23, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28110449/membrane-fluidity-controls-redox-regulated-cold-stress-responses-in-cyanobacteria
#16
Eugene G Maksimov, Kirill S Mironov, Marina S Trofimova, Natalya L Nechaeva, Daria A Todorenko, Konstantin E Klementiev, Georgy V Tsoraev, Eugene V Tyutyaev, Anna A Zorina, Pavel V Feduraev, Suleyman I Allakhverdiev, Vladimir Z Paschenko, Dmitry A Los
Membrane fluidity is the important regulator of cellular responses to changing ambient temperature. Bacteria perceive cold by the transmembrane histidine kinases that sense changes in thickness of the cytoplasmic membrane due to its rigidification. In the cyanobacterium Synechocystis, about a half of cold-responsive genes is controlled by the light-dependent transmembrane histidine kinase Hik33, which also partially controls the responses to osmotic, salt, and oxidative stress. This implies the existence of some universal, but yet unknown signal that triggers adaptive gene expression in response to various stressors...
January 21, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28070584/changes-in-protein-expression-profiles-in-bovine-endometrial-epithelial-cells-exposed-to-e-coli-lps-challenge
#17
Cristian Piras, Yongzhi Guo, Alessio Soggiu, Metasu Chanrot, Viviana Greco, Andrea Urbani, Gilles Charpigny, Luigi Bonizzi, Paola Roncada, Patrice Humblot
E. coli is one of the most frequently involved bacteria in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in pathogenic processes leading to post-partum metritis and endometritis in cattle. It also causes inflammation of the endometrium. The increase of cell proliferation by LPS is part of the inflammatory process. The aim of this study was to investigate possible changes in protein expression in relation to the proliferative response of bEECs after challenge with E...
January 10, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28066817/transcriptional-profiling-of-the-oral-pathogen-streptococcus-mutans-in-response-to-competence-signaling-peptide-xip
#18
Iwona B Wenderska, Andrew Latos, Benjamin Pruitt, Sara Palmer, Grace Spatafora, Dilani B Senadheera, Dennis G Cvitkovitch
In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S...
January 2017: MSystems
https://www.readbyqxmd.com/read/28040182/effects-of-exposure-to-quaternary-ammonium-based-biocides-on-antimicrobial-susceptibility-and-tolerance-to-physical-stresses-in-bacteria-from-organic-foods
#19
Rebeca Gadea, Miguel Ángel Fernández Fuentes, Rubén Pérez Pulido, Antonio Gálvez, Elena Ortega
In the present study, a collection of 76 biocide-sensitive bacterial strains isolated from organically produced food were adapted by repeated exposure to increasing concentrations of the quaternary ammonium compounds (QACs) benzalkonium chloride (BC) and hexadecylpyridinium chloride (HDP). The sensitivity of both wildtype strains and their corresponding QAC-adapted strains to other biocides and to antibiotics was studied. QAC tolerance increased in 88.2% of strains for BC and in 30.3% of strains for HDP, with increases in minimum inhibitory concentrations between 2 and over 100 fold...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28034666/oxidative-stress-in-sepsis-pathophysiological-implications-justifying-antioxidant-co-therapy
#20
REVIEW
Carlos André Prauchner
Sepsis is one of the main causes of death among critically ill patients. Sepsis pathogenesis includes infection by gram-negative and gram-positive bacteria, fungi, or both; exacerbated inflammatory response; hypotension, with potential to cause vasodilatory shock; and lesser delivery of oxygen to tissues due to impairment of oxygen utilization by cells. The participation of reactive species and/or free radicals such as nitric oxide (NO), peroxynitrite (ONOO(-)), superoxide (O2(-)), hydrogen peroxide (H2O2), and hydroxyl radical (OH) has been reported to underlie these effects...
December 26, 2016: Burns: Journal of the International Society for Burn Injuries
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