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stress response membrane bacteria

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https://www.readbyqxmd.com/read/28217741/transcriptome-level-signatures-in-gene-expression-and-gene-expression-variability-during-bacterial-adaptive-evolution
#1
Keesha E Erickson, Peter B Otoupal, Anushree Chatterjee
Antibiotic-resistant bacteria are an increasingly serious public health concern, as strains emerge that demonstrate resistance to almost all available treatments. One factor that contributes to the crisis is the adaptive ability of bacteria, which exhibit remarkable phenotypic and gene expression heterogeneity in order to gain a survival advantage in damaging environments. This high degree of variability in gene expression across biological populations makes it a challenging task to identify key regulators of bacterial adaptation...
January 2017: MSphere
https://www.readbyqxmd.com/read/28191904/n-terminomics-identifies-prli42-as-a-membrane-miniprotein-conserved-in-firmicutes-and-critical-for-stressosome-activation-in-listeria-monocytogenes
#2
Francis Impens, Nathalie Rolhion, Lilliana Radoshevich, Christophe Bécavin, Mélodie Duval, Jeffrey Mellin, Francisco García Del Portillo, M Graciela Pucciarelli, Allison H Williams, Pascale Cossart
To adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. In Gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma B regulon. The stressosome is structurally well-characterized in Bacillus, but how it senses stress remains elusive. Here, we report a genome-wide N-terminomic approach in Listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniproteins, among which one, Prli42, is conserved in Firmicutes...
February 13, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28177291/expression-of-flagellin-and-key-regulatory-flagellar-genes-in-the-non-motile-bacterium-piscirickettsia-salmonis
#3
Gabriela P Carril, Fernando A Gómez, Sergio H Marshall
The Piscirickettsia salmonis genome was screened to evaluate potential flagella-related open reading frames, as well as their genomic organization and eventual expression. A complete and organized set of flagellar genes was found for P. salmonis, although no structural flagellum has ever been reported for this bacterium. To gain further understanding, the hierarchical flagellar cascade described for Legionella pneumophila was used as a reference model for putative analysis in P. salmonis. Specifically, 5 of the most relevant genes from this cascade were chosen, including 3 regulatory genes (fleQ, triggers the cascade; fliA, regulates the σ28-coding gene; and rpoN, an RNA polymerase-dependent gene) and 2 terminal structural genes (flaA and flaB, flagellin and a flagellin-like protein, respectively)...
February 8, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28177232/adaptation-to-biocides-cetrimide-and-chlorhexidine-in-bacteria-from-organic-foods-association-with-tolerance-to-other-antimicrobials-and-physical-stresses
#4
Rebeca Gadea, Nicolas Glibota, Ruben Pérez Pulido, Antonio Galvez, Elena Ortega
Chlorhexidine (CH) and quaternary ammonium compounds (QAC), like cetrimide (CE) are widely used as disinfectants because of their broad antimicrobial spectrum. However, their frequent use for disinfection in different settings may promote bacterial drug resistance against both biocides and clinically relevant antibiotics. This study analyzes the effects of step-wise exposure to cetrimide (CE) and chlorhexidine (CH) of bacteria from organic foods and previously classified as sensitive to biocides. Gradual exposure of these strains to biocides resulted in mainly transient decreased antimicrobial susceptibility to other biocides and to antibiotics...
February 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28141494/butanol-is-cytotoxic-to-lactococcus-lactis-while-ethanol-and-hexanol-are-cytostatic
#5
Ann-Mette Meisner Hviid, Peter Rudahl Jensen, Mogens Kilstrup
Lactic acid bacteria currently used extensively by the dairy industry have a superior tolerance towards small chain alcohols, which makes them interesting targets for use in future bio-refineries. The mechanism underlying the alcohol tolerance of lactic acid bacteria has so far received little attention. In the present study the physiological alcohol stress response of Lactococcus lactis subsp. cremoris MG1363 towards the primary, even-chain alcohols; ethanol, butanol, and hexanol was characterized. The alcohol tolerance of L...
January 28, 2017: Microbiology
https://www.readbyqxmd.com/read/28131377/identification-of-otpr-regulated-srnas-in-brucella-melitensis-expressed-under-acidic-stress-and-their-roles-in-pathogenesis-and-metabolism
#6
Udayakumar S Vishnu, Jagadesan Sankarasubramanian, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Small RNAs (sRNAs) are the small regulatory molecules that regulate various biological processes in bacteria. Though the functions of sRNAs are well documented, very little information is available on the sRNAs of Brucella spp. The otpR is the response regulator of a two-component regulatory system, which plays a significant role in Brucella virulence. In this study, we identified the sRNAs expressed in B. melitensis 16M and its otpR mutant under acidic stress from the RNAseq dataset. We identified 94 trans-encoded and 948 cis-encoded sRNAs in B...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28115509/the-tat-substrate-sufi-is-critical-for-the-ability-of-yersinia-pseudotuberculosis-to-cause-systemic-infection
#7
Ummehan Avican, Tugrul Doruk, Yngve Östberg, Anna Fahlgren, Åke Forsberg
The Twin-arginine translocation (Tat) system targets folded proteins across the inner membrane and it is crucial for virulence in many important human pathogenic bacteria. Tat has been shown to be required for virulence of Yersinia pseudotuberculosis and we recently showed that the system is critical for different virulence related stress responses as well as for iron uptake. In this study, we wanted to address the role of the Tat substrates in in vivo virulence. Therefore, 22 genes encoding potential Tat substrates were mutated and each mutant was evaluated in competitive oral infection of mice...
January 23, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28110449/membrane-fluidity-controls-redox-regulated-cold-stress-responses-in-cyanobacteria
#8
Eugene G Maksimov, Kirill S Mironov, Marina S Trofimova, Natalya L Nechaeva, Daria A Todorenko, Konstantin E Klementiev, Georgy V Tsoraev, Eugene V Tyutyaev, Anna A Zorina, Pavel V Feduraev, Suleyman I Allakhverdiev, Vladimir Z Paschenko, Dmitry A Los
Membrane fluidity is the important regulator of cellular responses to changing ambient temperature. Bacteria perceive cold by the transmembrane histidine kinases that sense changes in thickness of the cytoplasmic membrane due to its rigidification. In the cyanobacterium Synechocystis, about a half of cold-responsive genes is controlled by the light-dependent transmembrane histidine kinase Hik33, which also partially controls the responses to osmotic, salt, and oxidative stress. This implies the existence of some universal, but yet unknown signal that triggers adaptive gene expression in response to various stressors...
January 21, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28070584/changes-in-protein-expression-profiles-in-bovine-endometrial-epithelial-cells-exposed-to-e-coli-lps-challenge
#9
Cristian Piras, Yongzhi Guo, Alessio Soggiu, Metasu Chanrot, Viviana Greco, Andrea Urbani, Gilles Charpigny, Luigi Bonizzi, Paola Roncada, Patrice Humblot
E. coli is one of the most frequently involved bacteria in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in pathogenic processes leading to post-partum metritis and endometritis in cattle. It also causes inflammation of the endometrium. The increase of cell proliferation by LPS is part of the inflammatory process. The aim of this study was to investigate possible changes in protein expression in relation to the proliferative response of bEECs after challenge with E...
January 10, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28066817/transcriptional-profiling-of-the-oral-pathogen-streptococcus-mutans-in-response-to-competence-signaling-peptide-xip
#10
Iwona B Wenderska, Andrew Latos, Benjamin Pruitt, Sara Palmer, Grace Spatafora, Dilani B Senadheera, Dennis G Cvitkovitch
In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S...
January 2017: MSystems
https://www.readbyqxmd.com/read/28040182/effects-of-exposure-to-quaternary-ammonium-based-biocides-on-antimicrobial-susceptibility-and-tolerance-to-physical-stresses-in-bacteria-from-organic-foods
#11
Rebeca Gadea, Miguel Ángel Fernández Fuentes, Rubén Pérez Pulido, Antonio Gálvez, Elena Ortega
In the present study, a collection of 76 biocide-sensitive bacterial strains isolated from organically produced food were adapted by repeated exposure to increasing concentrations of the quaternary ammonium compounds (QACs) benzalkonium chloride (BC) and hexadecylpyridinium chloride (HDP). The sensitivity of both wildtype strains and their corresponding QAC-adapted strains to other biocides and to antibiotics was studied. QAC tolerance increased in 88.2% of strains for BC and in 30.3% of strains for HDP, with increases in minimum inhibitory concentrations between 2 and over 100 fold...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28034666/oxidative-stress-in-sepsis-pathophysiological-implications-justifying-antioxidant-co-therapy
#12
REVIEW
Carlos André Prauchner
Sepsis is one of the main causes of death among critically ill patients. Sepsis pathogenesis includes infection by gram-negative and gram-positive bacteria, fungi, or both; exacerbated inflammatory response; hypotension, with potential to cause vasodilatory shock; and lesser delivery of oxygen to tissues due to impairment of oxygen utilization by cells. The participation of reactive species and/or free radicals such as nitric oxide (NO), peroxynitrite (ONOO(-)), superoxide (O2(-)), hydrogen peroxide (H2O2), and hydroxyl radical (OH) has been reported to underlie these effects...
December 26, 2016: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28030630/system-wide-adaptations-of-desulfovibrio-alaskensis-g20-to-phosphate-limited-conditions
#13
Tanja Bosak, Florence Schubotz, Ana de Santiago-Torio, Jennifer V Kuehl, Hans K Carlson, Nicki Watson, Mirna Daye, Roger E Summons, Adam P Arkin, Adam M Deutschbauer
The prevalence of lipids devoid of phosphorus suggests that the availability of phosphorus limits microbial growth and activity in many anoxic, stratified environments. To better understand the response of anaerobic bacteria to phosphate limitation and starvation, this study combines microscopic and lipid analyses with the measurements of fitness of pooled barcoded transposon mutants of the model sulfate reducing bacterium Desulfovibrio alaskensis G20. Phosphate-limited G20 has lower growth rates and replaces more than 90% of its membrane phospholipids by a mixture of monoglycosyl diacylglycerol (MGDG), glycuronic acid diacylglycerol (GADG) and ornithine lipids, lacks polyphosphate granules, and synthesizes other cellular inclusions...
2016: PloS One
https://www.readbyqxmd.com/read/28027983/effect-of-ammonia-n-and-pathogen-challenge-on-complement-component-8%C3%AE-and-8%C3%AE-expression-in-the-darkbarbel-catfish-pelteobagrus-vachellii
#14
Chuanjie Qin, Ting Shao, Daxian Zhao, Huiguo Duan, Zhengyong Wen, Dengyue Yuan, Huatao Li, Zemin Qi
The complement components C8α and C8β mediate the formation of the membrane attack complex (MAC) to resist pathogenic bacteria and play important roles in innate immunity. Full-length complement C8α (Pv-C8α) and C8β (Pv-C8β) cDNA were identified in the darkbarbel catfish Pelteobagrus vachellii, and their mRNA expression levels were analyzed after ammonia-N and pathogen treatment. The Pv-C8α gene contained 1983 bp, including a 1794-bp open reading frame (ORF) encoding 598 amino acids. The Pv-C8β gene contained 1952 bp, including a 1761-bp ORF encoding 587 amino acids...
December 25, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27999164/cytochrome-bd-dependent-bioenergetics-and-antinitrosative-defenses-in-salmonella-pathogenesis
#15
Jessica Jones-Carson, Maroof Husain, Lin Liu, David J Orlicky, Andrés Vázquez-Torres
: In the course of an infection, Salmonella enterica occupies diverse anatomical sites with various concentrations of oxygen (O2) and nitric oxide (NO). These diatomic gases compete for binding to catalytic metal groups of quinol oxidases. Enterobacteriaceae express two evolutionarily distinct classes of quinol oxidases that differ in affinity for O2 and NO as well as stoichiometry of H(+) translocated across the cytoplasmic membrane. The investigations presented here show that the dual function of bacterial cytochrome bd in bioenergetics and antinitrosative defense enhances Salmonella virulence...
December 20, 2016: MBio
https://www.readbyqxmd.com/read/27995790/physiological-responses-of-salinity-stressed-vibrio-sp-and-the-effect-on-the-biofilm-formation-on-a-nanofiltration-membrane
#16
Lan Hee Kim, Tzyy Haur Chong
This study evaluated the effects of salinity on the physiological characteristics of Vibrio sp. B2 and biofilm formation on nanofiltration (NF) membrane coupons used in the high recovery seawater desalination process. The test conditions were at 0.6, 1.2, and 2.4 M sodium chloride (NaCl), equivalent to salinity of seawater, brine at 50% and 75% water recovery, respectively. High salinity inhibited the cell growth rate but increased the viability and bacterial membrane integrity. In addition, protein and eDNA concentrations of salinity-stressed bacteria were increased at 1...
January 11, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27965646/synthesis-of-sphingolipids-impacts-survival-of-porphyromonas-gingivalis-and-the-presentation-of-surface-polysaccharides
#17
Zachary D Moye, Kornelija Valiuskyte, Floyd E Dewhirst, Frank C Nichols, Mary E Davey
Bacteria alter the biophysical properties of their membrane lipids in response to environmental cues, such as shifts in pH or temperature. In essence, lipid composition determines membrane structure, which in turn influences many basic functions, such as transport, secretion, and signaling. Like other members of the phylum Bacteroidetes, the oral anaerobe Porphyromonas gingivalis possesses the ability to synthesize a variety of novel membrane lipids, including species of dihydroceramides that are distinct, yet similar in structure to sphingolipids produced by the human host...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27942460/transcription-profiling-data-set-of-different-states-of-mycoplasma-gallisepticum
#18
Tatiana A Semashko, Alexander A Arzamasov, Gleb Y Fisunov, Vadim M Govorun
Mycoplasma gallisepticum belongs to class Mollicutes and causes chronic respiratory disease in birds. It has a reduced genome, lack of cell wall and many metabolic pathways, and also easy to culture and non-pathogenic to humans. Aforementioned made it is a convenient model for studying of systems biology of minimal cell. Studying the transcriptomic level of M. gallisepticum is interesting for both understanding of common principles of transcription regulation of minimal cell and response to definite influence for pathogen bacteria...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/27941972/a-kinase-phosphatase-switch-transduces-environmental-information-into-a-bacterial-cell-cycle-circuit
#19
Kristina Heinrich, Patrick Sobetzko, Kristina Jonas
The bacterial cell cycle has been extensively studied under standard growth conditions. How it is modulated in response to environmental changes remains poorly understood. Here, we demonstrate that the freshwater bacterium Caulobacter crescentus blocks cell division and grows to filamentous cells in response to stress conditions affecting the cell membrane. Our data suggest that stress switches the membrane-bound cell cycle kinase CckA to its phosphatase mode, leading to the rapid dephosphorylation, inactivation and proteolysis of the master cell cycle regulator CtrA...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27936405/identification-of-lipopolysaccharide-interacting-plasma-membrane-type-proteins-in-arabidopsis-thaliana
#20
Cornelius S Vilakazi, Ian A Dubery, Lizelle A Piater
Lipopolysaccharide (LPS) is an amphiphatic bacterial glycoconjugate found on the external membrane of Gram-negative bacteria. This endotoxin is considered as a microbe-associated molecular pattern (MAMP) molecule and has been shown to elicit defense responses in plants. Here, LPS-interacting proteins from Arabidopsis thaliana plasma membrane (PM)-type fractions were captured and identified in order to investigate those involved in LPS perception and linked to triggering of innate immune responses. A novel proteomics-based affinity-capture strategy coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed for the enrichment and identification of LPS-interacting proteins...
November 30, 2016: Plant Physiology and Biochemistry: PPB
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