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JOURNAL ARTICLE
Idan Carmon, Shira Kalmus, Anna Zobrab, Michael Alterman, Raphaelle Emram, May Gussarsky, Leonid Kandel, Eli Reich, Nardi Casap, Mona Dvir-Ginzberg
In cranial flat bone fractures, spontaneous bone repair will occur only when the fracture ends are in close contact. However, in cases wherein bone discontinuity is extensive, surgical interventions are often required. To this end, autologous bone is harvested and surgically integrated into the site of fracture. Here we propose to use cartilage, as an alternative autologous source, to promote cranial fracture repair. The advantage of this approach is the potential reduction in donor site morbidity, likely due to the avascular and aneural nature of cartilage...
2023: Journal of Tissue Engineering
#22
REVIEW
Yuqi Zheng, Manuel B Graeber
Evidence is accumulating that the tumour microenvironment (TME) has a key role in the progression of gliomas. Non-neoplastic cells in addition to the tumour cells are therefore finding increasing attention. Microglia and other glioma-associated macrophages are at the centre of this interest especially in the context of therapeutic considerations. New ideas have emerged regarding the role of microglia and, more recently, blood-derived brain macrophages in glioblastoma (GBM) progression. We are now beginning to understand the mechanisms that allow malignant glioma cells to weaken microglia and brain macrophage defence mechanisms...
December 9, 2022: International Journal of Molecular Sciences
#23
JOURNAL ARTICLE
Taotao Ji, Xiang Chen, Yan Zhang, Kaibin Fu, Yue Zou, Weiwei Wang, Jiafu Zhao
The purpose of this paper was to investigate the effects of N-acetylcysteine (NAC) on the proliferation, hormone secretion, and mRNA expression profiles of ovarian granulosa cells (GCs) in vitro. A total of 12 ovaries from 6 follicular-stage goats were collected for granulosa cell extraction. The optimum concentration of NAC addition was determined to be 200 μM via the Cell Counting Kit 8 (CCK-8) method. Next, GCs were cultured in a medium supplemented with 200 μM NAC (200 μM NAC group) and 0 μ M NAC (control group) for 48 h...
December 7, 2022: Genes
#24
JOURNAL ARTICLE
Ritchie G M Timmermans, Arjen B Blom, Niek G C Bloks, Rob G H H Nelissen, Enrike H M J van der Linden, Peter M van der Kraan, Ingrid Meulenbelt, Yolande F M Ramos, Martijn H J van den Bosch
OBJECTIVES: Previously, we have shown the involvement of cellular communication network factor 4/Wnt-activated protein Wnt-1-induced signaling protein 1 (CCN4/WISP1) in osteoarthritic (OA) cartilage and its detrimental effects on cartilage. Here, we investigated characteristics of CCN4 in chondrocyte biology by exploring correlations of CCN4 with genes expressed in human OA cartilage with functional follow-up. DESIGN: Spearman correlation analysis was performed for genes correlating with CCN4 using our previously established RNA sequencing dataset of human preserved OA cartilage of the RAAK study, followed by a pathway enrichment analysis for genes with ρ ≥|0...
December 22, 2022: Cartilage
#25
JOURNAL ARTICLE
Lisa C Green, Samuel Slone, Sarah R Anthony, Adrienne R Guarnieri, Sharon Parkins, Shannon M Shearer, Michelle L Nieman, Sudeshna Roy, Jeffrey Aube, Xiaoqing Wu, Liang Xu, Onur Kanisicak, Michael Tranter
Cardiac fibrosis is regulated by the activation and phenotypic switching of quiescent cardiac fibroblasts to active myofibroblasts, which have extracellular matrix (ECM) remodeling and contractile functions which play a central role in cardiac remodeling in response to injury. Here, we show that expression and activity of the RNA binding protein HuR is increased in cardiac fibroblasts upon transformation to an active myofibroblast. Pharmacological inhibition of HuR significantly blunts the TGFβ-dependent increase in ECM remodeling genes, total collagen secretion, in vitro scratch closure, and collagen gel contraction in isolated primary cardiac fibroblasts, suggesting a suppression of TGFβ-induced myofibroblast activation upon HuR inhibition...
November 11, 2022: Journal of Molecular and Cellular Cardiology
#26
JOURNAL ARTICLE
Martijn H J van den Bosch, Esmeralda N Blaney Davidson
The matricellular protein Wnt-induced secreted protein 1 (WISP1) is the fourth member of the CCN family of proteins, which has been shown to affect tissues of the musculoskeletal system. In the context of the musculoskeletal disorder osteoarthritis, our lab studied the function of CCN4/WISP1 in joint tissues, including synovium and cartilage, using both gain- and loss-of-function approaches. In mice, this was done by genetic engineering and recombination to generate mice deficient in CCN4/WISP1 protein. Various experimental models of osteoarthritis with different characteristics were induced in these mice...
2023: Methods in Molecular Biology
#27
JOURNAL ARTICLE
Rebeca Fernández-Ruiz, Rosa Gasa
Expanding the number of insulin-producing beta cells through reactivation of their replication has been proposed as a therapy to prevent or delay the appearance of diabetes. Using antibody arrays, we identified CCN4/Wisp1 as a circulating factor enriched in preweaning mice, a period in which beta cells exhibit a dramatic increase in number. This finding led us to investigate the involvement of CCN4 in beta cell proliferation. We demonstrated that CCN4 promotes adult beta cell proliferation in vitro in cultured isolated islets, and in vivo in islets transplanted into the anterior chamber of the eye...
2023: Methods in Molecular Biology
#28
JOURNAL ARTICLE
Yuka Okusha, Takanori Eguchi
The Cellular Communication Network Factor (CCN) family is composed of six members: CCN1/CYR61, CCN2/CTGF, CCN3/NOV, CCN4/WISP1, CCN5/WISP2, and CCN6/WISP3. The second member, CCN2/CTGF is a matricellular protein that promotes extracellular matrix (ECM) synthesis and controls angiogenesis. On the other hand, moonlighting/matrix metalloproteinase 3 (MMP3) is an ECM-degrading enzyme that also functions as an intracellular transcription factor. Importantly, extracellular MMP3 is uptaken into cells, translocating into nuclei, and transcriptionally activating CCN2/CTGF gene in cancer and chondrocytes...
2023: Methods in Molecular Biology
#29
JOURNAL ARTICLE
Pierre-Olivier Gaudreau, Isabelle Cousineau, John Stagg
CCN4 (also known as WNT1-Inducible Signaling Pathway Protein 1 or WISP1) is a 367 amino acid, 40 kDa protein located on chromosome 8q24.1-8q24.3. Prior studies have provided support for a pro-inflammatory role for CCN4. We have shown recently that CCN4 expression is associated with advanced disease, epithelial-mesenchymal transition, and an inflamed tumor microenvironment in multiple solid tumors. We detail here the CCN4 tissue microarray immunofluorescence protocol related to these findings.
2023: Methods in Molecular Biology
#30
JOURNAL ARTICLE
Kazuyoshi Ishigaki, Saori Sakaue, Chikashi Terao, Yang Luo, Kyuto Sonehara, Kensuke Yamaguchi, Tiffany Amariuta, Chun Lai Too, Vincent A Laufer, Ian C Scott, Sebastien Viatte, Meiko Takahashi, Koichiro Ohmura, Akira Murasawa, Motomu Hashimoto, Hiromu Ito, Mohammed Hammoudeh, Samar Al Emadi, Basel K Masri, Hussein Halabi, Humeira Badsha, Imad W Uthman, Xin Wu, Li Lin, Ting Li, Darren Plant, Anne Barton, Gisela Orozco, Suzanne M M Verstappen, John Bowes, Alexander J MacGregor, Suguru Honda, Masaru Koido, Kohei Tomizuka, Yoichiro Kamatani, Hiroaki Tanaka, Eiichi Tanaka, Akari Suzuki, Yuichi Maeda, Kenichi Yamamoto, Satoru Miyawaki, Gang Xie, Jinyi Zhang, Christopher I Amos, Edward Keystone, Gertjan Wolbink, Irene van der Horst-Bruinsma, Jing Cui, Katherine P Liao, Robert J Carroll, Hye-Soon Lee, So-Young Bang, Katherine A Siminovitch, Niek de Vries, Lars Alfredsson, Solbritt Rantapää-Dahlqvist, Elizabeth W Karlson, Sang-Cheol Bae, Robert P Kimberly, Jeffrey C Edberg, Xavier Mariette, Tom Huizinga, Philippe Dieudé, Matthias Schneider, Martin Kerick, Joshua C Denny, Koichi Matsuda, Keitaro Matsuo, Tsuneyo Mimori, Fumihiko Matsuda, Keishi Fujio, Yoshiya Tanaka, Atsushi Kumanogoh, Matthew Traylor, Cathryn M Lewis, Stephen Eyre, Huji Xu, Richa Saxena, Thurayya Arayssi, Yuta Kochi, Katsunori Ikari, Masayoshi Harigai, Peter K Gregersen, Kazuhiko Yamamoto, S Louis Bridges, Leonid Padyukov, Javier Martin, Lars Klareskog, Yukinori Okada, Soumya Raychaudhuri
Rheumatoid arthritis (RA) is a highly heritable complex disease with unknown etiology. Multi-ancestry genetic research of RA promises to improve power to detect genetic signals, fine-mapping resolution and performances of polygenic risk scores (PRS). Here, we present a large-scale genome-wide association study (GWAS) of RA, which includes 276,020 samples from five ancestral groups. We conducted a multi-ancestry meta-analysis and identified 124 loci (P < 5 × 10-8 ), of which 34 are novel...
November 2022: Nature Genetics
#31
EDITORIAL
Kenneth Maiese
No abstract text is available yet for this article.
October 19, 2022: Current Neurovascular Research
#32
JOURNAL ARTICLE
A S Belyashova, M V Galkin, N A Antipina, G V Pavlova, A V Golanov
To date, no modern methods of treatment allow overcoming malignant potential of glial neoplasms and significant increase of survival. Analysis of glioblastoma radioresistance using cancer cell cultures is one of the perspective directions, as radiotherapy is standard and available treatment method for these neoplasms. This review summarizes current studies identifying many factors of radioresistance of glial tumors, such as hypoxia, microenvironment and metabolic features of tumor, stem cells, internal heterogeneity of tumor, microRNA, features of cell cycle, DNA damage and reparation...
2022: Zhurnal Voprosy Neĭrokhirurgii Imeni N. N. Burdenko
#33
JOURNAL ARTICLE
Kang-Shuo Chang, Syue-Ting Chen, Hsin-Ching Sung, Shu-Yuan Hsu, Wei-Yin Lin, Chen-Pang Hou, Yu-Hsiang Lin, Tsui-Hsia Feng, Ke-Hung Tsui, Horng-Heng Juang
The WNT1 inducible signaling pathway protein 1 (WISP1), a member of the connective tissue growth factor family, plays a crucial role in several important cellular functions in a highly tissue-specific manner. Results of a RT-qPCR indicated that WISP1 expressed only in cells of the human prostate fibroblasts, HPrF and WPMY-1, but not the prostate carcinoma cells in vitro. Two major isoforms (WISP1v1 and WISP1v2) were identified in the HPrF cells determined by RT-PCR and immunoblot assays. The knock-down of a WISP1 blocked cell proliferation and contraction, while treating respectively with the conditioned medium from the ectopic WISP1v1- and WISPv2-overexpressed 293T cells enhanced the migration of HPrF cells...
September 28, 2022: International Journal of Molecular Sciences
#34
JOURNAL ARTICLE
Kai Ai, Lei Yi, Yinhuai Wang, Yuan Li
Growing evidence suggest that circular RNAs (circRNAs) are critical mediators in renal diseases. However, there have been very few reports about the role of circRNAs in renal fibrosis. In this study, circRNA_33702 was found to be upregulated, both in UUO mice and in TGF-β1-treated BUMPT cells. Furthermore, hsa_circ_0026331, homologous with mmu_circ_33702, was found to be upregulated in TGF-β1-treated HK-2 cells. Whilst knockdown of circRNA_33702 or hsa_circ_0026331 was shown to relieve the TGF-β1-induced expression of collagen I, collagen Ⅲ and fibronectin; overexpression of circRNA_33702 was found to exert an inhibitory effect on the expression of the same genes...
September 24, 2022: Journal of Pharmacology and Experimental Therapeutics
#35
JOURNAL ARTICLE
Chen Wei, Yixiang Liu, Enhong Xing, Zhenjiang Ding, Yanan Tian, Zhuoyan Zhao, Wenjun Fan, Lixian Sun
BACKGROUND AND AIMS: Novel pro- and anti-inflammatory adipocytokines affect inflammation, energy metabolism, and insulin signaling. However, their role in acute coronary syndrome (ACS) development is unclear. We evaluated the diagnostic and risk predictive value of such adipocytokines for ACS. METHODS: We enrolled 168 consecutive inpatients with suspected ACS and detected serum PLIN1, PLIN2, PLIN5, CTRP6, CTRP7, CTRP11, WISP1, FAM19A5, TNF-α, and adiponectin levels...
January 2022: Clinical and Applied Thrombosis/hemostasis
#36
JOURNAL ARTICLE
Qian-Nan Zhang, Huan Xiao, Li-Ting Fang, Qi-Xiang Sun, Lao-Dong Li, Si-Yue Xu, Chao-Qian Li
Background: Airway remodeling is accepted to be a determining component within the natural history of asthma. Nebulized inhalation of Mycobacterium vaccae ( M. vaccae ) has a protective effect on asthmatic mice. However, little is known regarding the effect of M. vaccae on airway structural remodeling in asthmatic mice. The purpose of this study was to explore the effect and the underlying mechanism of M. vaccae aerosol inhalation on airway structural remodeling in an asthma mouse model. Methods: Chronic asthma mouse models were established by ovalbumin induction...
August 24, 2022: Experimental Lung Research
#37
JOURNAL ARTICLE
Ying Xi, Ryan LaCanna, Hsiao-Yen Ma, Elsa-Noah N'Diaye, Sarah Gierke, Patrick Caplazi, Meredith Sagolla, Zhiyu Huang, Laura Lucio, Alexander Arlantico, Surinder Jeet, Hans Brightbill, Claire Emson, Aaron Wong, Katrina B Morshead, Daryle J DePianto, Merone Roose-Girma, Charles Yu, Lucinda Tam, Guiquan Jia, Thirumalai R Ramalingam, Scot Marsters, Avi Ashkenazi, Si Hyun Kim, Ryan Kelly, Shuang Wu, Paul J Wolters, Ariel E Feldstein, Jason A Vander Heiden, Ning Ding
Fibrosis is the major risk factor associated with morbidity and mortality in patients with non-alcoholic steatohepatitis (NASH)-driven chronic liver disease. Although numerous efforts have been made to identify the mediators of the initiation of liver fibrosis, the molecular underpinnings of fibrosis progression remain poorly understood, and therapies to arrest liver fibrosis progression are elusive. Here, we identify a pathway involving WNT1-inducible signaling pathway protein 1 (WISP1) and myocardin-related transcription factor (MRTF) as a central mechanism driving liver fibrosis progression through the integrin-dependent transcriptional reprogramming of myofibroblast cytoskeleton and motility...
September 6, 2022: Cell Metabolism
#38
REVIEW
Jia-Xue Cheng, Ke Yu
Obesity is defined as abnormal or excessive accumulation of adipose tissue, closely associated with the increased risk of various comorbidities, especially type 2 diabetes mellitus (T2DM). Adipose tissue is a complex structure responsible for not only fat storage but also releasing adipokines which may play roles in the pathogenesis and could be developed into biomarkers for diagnosis, treatment and prognosis of obesity-related metabolic diseases. This review aims to summarize several adipokines discovered recently that have promising functions in obesity and T2DM...
2022: Diabetes, Metabolic Syndrome and Obesity
#39
JOURNAL ARTICLE
Yan Li, Fangfang Wang, Tianyi Liu, Nan Lv, Xiaolei Yuan, Peiling Li
BACKGROUND: This study intended to clarify the mechanisms by which WISP1-mediated IGF1/αvβ3/Wnt axis might affect the progression of ovarian cancer. METHODS: Bioinformatics analysis was implemented for pinpointing expression of IGF1 and WISP1 which was verified through expression determination in clinical tissue samples and cells. Next, gain- or loss-of-function experimentations were implemented for testing CAOV4 and SKOV3 cell biological processes. The interaction between WISP1 and IGF1 was verified by co-immunoprecipitation and the molecular mechanism was analyzed...
August 13, 2022: Journal of Ovarian Research
#40
REVIEW
Yakun Ren, Hao Zhao, Chunyan Yin, Xi Lan, Litao Wu, Xiaojuan Du, Helen R Griffiths, Dan Gao
Chronic low-grade inflammation in adipose tissue (AT) is a hallmark of obesity and contributes to various metabolic disorders, such as type 2 diabetes and cardiovascular diseases. Inflammation in ATs is characterized by macrophage infiltration and the activation of inflammatory pathways mediated by NF-κB, JNK, and NLRP3 inflammasomes. Adipokines, hepatokines and myokines - proteins secreted from AT, the liver and skeletal muscle play regulatory roles in AT inflammation via endocrine, paracrine, and autocrine pathways...
2022: Frontiers in Endocrinology
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