keyword
MENU ▼
Read by QxMD icon Read
search

Mesenchymal stromal cell

keyword
https://www.readbyqxmd.com/read/28632184/crude-fucoidan-extracts-impair-angiogenesis-in-models-relevant-for-bone-regeneration-and-osteosarcoma-via-reduction-of-vegf-and-sdf-1
#1
Fanlu Wang, Harald Schmidt, Dijana Pavleska, Thees Wermann, Andreas Seekamp, Sabine Fuchs
The marine origin polysaccharide fucoidan combines multiple biological activities. As demonstrated by various studies in vitro and in vivo, fucoidans show anti-viral, anti-tumor, anti-oxidant, anti-inflammatory and anti-coagulant properties, although the detailed molecular action remains to be elucidated. The aim of the present study is to assess the impact of crude fucoidan extracts, on the formation of vascular structures in co-culture models relevant for bone vascularization during bone repair and for vascularization processes in osteosarcoma...
June 20, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28631613/repairing-a-bone-defect-with-a-three-dimensional-cellular-construct-composed-of-a-multi-layered-cell-sheet-on-electrospun-mesh
#2
Zhiwei Ren, Shiqing Ma, Le Jin, Zihao Liu, Deping Liu, Xu Zhang, Qing Cai, Xiaoping Yang
In addition to providing maneuverability, electrospun nanofibrous meshes can make excellent supports for constructing flexible cell sheets to regulate cell behavior by nanofiber features. With the target of bone regeneration, herein composite nanofibers with two different fiber arrangements (nestlike, random) were electrospun from a blend solution containing poly(l-lactide) (PLLA) and gelatin (1:1 in weight ratio). Unlike the non-woven morphology in a random nanofibrous mesh, PLLA/gelatin composite nanofibers in the nestlike nanofibrous mesh displayed both non-woven and parallel morphologies...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28631500/pancreatic-%C3%AE-cell-survival-and-proliferation-are-promoted-by-protein-kinase-g-type-i%C3%AE-and-downstream-regulation-of-akt-foxo1
#3
Janica C Wong, Van Vo, Priyatham Gorjala, Ronald R Fiscus
Early studies showed nitric oxide as a pro-inflammatory-cytokine-induced toxin involved in pancreatic β-cell destruction during pathogenesis of type-1 diabetes. However, nitric oxide has both cytotoxic and cytoprotective effects on mammalian cells, depending on concentration and micro-environmental surroundings. Our studies have shown that low/physiological-level nitric oxide selectively activates protein kinase G type Iα isoform, promoting cytoprotective/pro-cell-survival effects in many cell types. In bone marrow-derived stromal/mesenchymal stem cells, protein kinase G type Iα mediates autocrine effects of nitric oxide and atrial natriuretic peptide, promoting DNA-synthesis/proliferation and cell survival...
June 1, 2017: Diabetes & Vascular Disease Research
https://www.readbyqxmd.com/read/28630279/obesity-and-p16-ink4a-down-regulation-activate-breast-adipocytes-and-promote-their-pro-tumorigenicity
#4
Huda H Al-Khalaf, Mrad Amir, Falah Al-Mohanna, Asma Tulbah, Adher Al-Sayed, Abdelilah Aboussekhra
Obesity is increasingly recognized as a risk factor for breast cancer development. However, the molecular basis of obesity-related breast carcinogenesis remains elusive. In this study, we have shown that obesity reduces the level of the tumor suppressor p16(INK4A) protein in breast adipocytes, which showed active features and strong pro-carcinogenic potential both in vitro and in orthotopic tumor xenografts, as compared to mature adipocytes from lean women. Furthermore, obesity triggered epithelial-to-mesenchymal transition (EMT) in breast ductal epithelial cells...
June 19, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28630143/extracellular-vesicles-of-stromal-origin-target-and-support-hematopoietic-stem-and-progenitor-cells
#5
Gregoire Stik, Simon Crequit, Laurence Petit, Jennifer Durant, Pierre Charbord, Thierry Jaffredo, Charles Durand
Extracellular vesicles (EVs) have been recently reported as crucial mediators in cell-to-cell communication in development and disease. In this study, we investigate whether mesenchymal stromal cells that constitute a supportive microenvironment for hematopoietic stem and progenitor cells (HSPCs) released EVs that could affect the gene expression and function of HSPCs. By taking advantage of two fetal liver-derived stromal lines with widely differing abilities to maintain HSPCs ex vivo, we demonstrate that stromal EVs play a critical role in the regulation of HSPCs...
June 19, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28627713/process-development-of-human-multipotent-stromal-cell-microcarrier-culture-using-an-automated-high-throughput-microbioreactor
#6
Qasim A Rafiq, Mariana P Hanga, Thomas R J Heathman, Karen Coopman, Alvin W Nienow, David J Williams, Christopher J Hewitt
Microbioreactors play a critical role in process development as they reduce reagent requirements and can facilitate high-throughput screening of process parameters and culture conditions. Here we have demonstrated and explained in detail, for the first time, the amenability of the automated ambr15 cell culture microbioreactor system for the development of scalable adherent human mesenchymal multipotent stromal/stem cell (hMSC) microcarrier culture processes. This was achieved by first improving suspension and mixing of the microcarriers and then improving cell attachment thereby reducing the initial growth lag phase...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28625827/cells-as-advanced-therapeutics-state-of-the-art-challenges-and-opportunities-in-large-scale-biomanufacturing-of-high-quality-cells-for-adoptive-immunotherapies
#7
Nate J Dwarshuis, Kirsten Parratt, Adriana Santiago-Miranda, Krishnendu Roy
Therapeutic cells hold tremendous promise in treating currently incurable, chronic diseases since they perform multiple, integrated, complex functions in vivo compared to traditional small-molecule drugs or biologics. However, they also pose significant challenges as therapeutic products because (a) their complex mechanisms of actions are difficult to understand and (b) low-cost bioprocesses for large-scale, reproducible manufacturing of cells have yet to be developed. Immunotherapies using T cells and dendritic cells (DCs) have already shown great promise in treating several types of cancers, and human mesenchymal stromal cells (hMSCs) are now extensively being evaluated in clinical trials as immune-modulatory cells...
June 15, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/28622526/extragastrointestinal-stromal-tumour-of-the-lesser-omentum-a-case-report-and-literature-review
#8
I E Katsoulis, A Tzortzopoulou, P Tziakou, N Arnogiannaki, I Kostoglou-Athanassiou, G Lypas, I G Karaitianos
INTRODUCTION: Extragastrointestinal stromal tumours (EGISTs) are very uncommon compared to their gastrointestinal counterparts. Most of them originate from the intestinal mesentery and the omentum. CASE REPORT: A 70 year-old Caucasian woman presented with a bulky abdominal mass which on laparotomy was found to originate from the lesser omentum and was completely resected. Histological examination revealed spindle cells with severe pleomorphism and high mitotic activity...
June 8, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28622233/human-mesenchymal-stem-stromal-cells-from-human-umbilical-cord-ameliorate-acute-respiratory-distress-syndrome-in-rats-factors-to-consider
#9
Yan-Yan Xuan, Yi-Yi Wu, Yi-Lian Xie, Jin-Guo Chu, Guo-Xiang Li, Li-Ping Wang
No abstract text is available yet for this article.
July 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28621294/endoscopic-ultrasound-with-tissue-sampling-is-accurate-in-the-diagnosis-and-subclassification-of-gastrointestinal-spindle-cell-neoplasms
#10
Dalbir Singh Sandhu, Adrian N Holm, Rami El-Abiad, Carolyn Rysgaard, Chris Jensen, Henning Gerke
BACKGROUND AND OBJECTIVES: Mesenchymal (spindle cell) neoplasms (SCN) of the gastrointestinal (GI) tract are an important subtype of subepithelial lesions that need subclassification to assess their malignant potential. Reported success rates of accurate subclassification with endoscopic ultrasound (EUS)-guided biopsies are variable. Our goal was to analyze our experience using EUS-guided TruCut biopsy (EUS-TCB) in the majority of patients. METHODS: Retrospective analysis in patients who underwent EUS with biopsies for suspected SCN at our tertiary referral center between 2004 and 2013...
May 2017: Endoscopic Ultrasound
https://www.readbyqxmd.com/read/28620864/adipose-tissue-derived-stromal-cells-adsc-express-oligodendrocyte-and-myelin-markers-but-they-do-not-function-as-oligodendrocytes
#11
Lara Vellosillo, Maria Paz Muñoz, Carlos Luis Paíno
Mesenchymal cells cultured from the vasculo-stromal fraction of adipose tissue (ADSC) show adult stem cell characteristics and several groups have claimed generating neural cells from them. However, we have observed that many markers commonly used for the identification of neural cells are spontaneously expressed by ADSC in culture. In the present study, we have examined the expression of characteristic oligodendrocyte molecules in cultured ADSC, aiming to test if myelinating cells could be generated from accessible non-neural adult tissues...
June 15, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28620644/topical-application-of-mesenchymal-stromal-cells-ameliorated-liver-parenchyma-damage-after-ischemia-reperfusion-injury-in-an-animal-model
#12
Ping Kuen Lam, Charing Ching Ning Chong, Anthony Wing Ip Lo, Anthony Wing Hung Chan, Cindy See Wai Tong, Don Wai Ching Chin, Kenneth Hoi Kin Wong, Richard Kwong Wai Choy, Andrew Kai-Yip Fung, Yi Xiang Wang, Ka Fai To, Paul Bo San Lai
BACKGROUND: Ischemia-reperfusion injury (IRI) is commonly encountered after liver surgery. This study evaluated the hepatoprotective effects of topically applied adipose-derived mesenchymal stromal cells (ADMSCs) on hepatic IRI in a rat model. METHODS: ADMSCs from transgenic green fluorescent protein Sprague-Dawley rats were topically applied to the liver surface of Sprague-Dawley rats after hepatic IRI and fixed in position by fibrin glue (group A, n = 24). An equivalent amount of ADMSCs were administered through the portal (group B, n = 24) or tail vein (group C, n = 24)...
June 2017: Transplantation Direct
https://www.readbyqxmd.com/read/28618870/molecular-validation-of-chondrogenic-differentiation-and-hypoxia-responsiveness-of-platelet-lysate-expanded-adipose-tissue-derived-human-mesenchymal-stromal-cells
#13
Catalina Galeano-Garces, Emily T Camilleri, Scott M Riester, Amel Dudakovic, Dirk R Larson, Wenchun Qu, Jay Smith, Allan B Dietz, Hee-Jeong Im, Aaron J Krych, A Noelle Larson, Marcel Karperien, Andre J van Wijnen
OBJECTIVE: To determine the optimal environmental conditions for chondrogenic differentiation of human adipose tissue-derived mesenchymal stromal/stem cells (AMSCs). In this investigation we specifically investigate the role of oxygen tension and 3-dimensional (3D) culture systems. DESIGN: Both AMSCs and primary human chondrocytes were cultured for 21 days in chondrogenic media under normoxic (21% oxygen) or hypoxic (2% oxygen) conditions using 2 distinct 3D culture methods (high-density pellets and poly-ε-caprolactone [PCL] scaffolds)...
July 2017: Cartilage
https://www.readbyqxmd.com/read/28615711/identification-and-characterization-of-a-rich-population-of-cd34-mesenchymal-stem-stromal-cells-in-human-parotid-sublingual-and-submandibular-glands
#14
Padma Priya Togarrati, Robson T Sasaki, Mohamed Abdel-Mohsen, Nuntana Dinglasan, Xutao Deng, Shivani Desai, Elaine Emmerson, Elizabeth Yee, William R Ryan, Marcelo C P da Silva, Sarah M Knox, Satish K Pillai, Marcus O Muench
Mesenchymal stem/stromal cells (MSCs) play crucial roles in maintaining tissue homeostasis during physiological turnovers and injuries. Very little is known about the phenotype, distribution and molecular nature of MSCs in freshly isolated human salivary glands (SGs) as most reports have focused on the analysis of cultured MSCs. Our results demonstrate that the cell adhesion molecule CD34 was widely expressed by the MSCs of human major SGs, namely parotid (PAG), sublingual (SLG) and submandibular (SMG) glands...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28612255/il-27-regulates-the-adherence-proliferation-and-migration-of-mscs-and-enhances-their-regulatory-effects-on-th1-and-th2-subset-generations
#15
Fenghuang Xu, Junzhu Yi, Zhuoya Wang, Yejia Hu, Chunlei Han, Qun Xue, Xueguang Zhang, Xiying Luan
Interleukin 27 (IL-27) regulates T cell function and is involved in inflammation. It has been reported that human placenta-derived mesenchymal stromal cells (hPMSCs) can inhibit T cell responses and attenuate inflammation reactions. However, it is unclear whether IL-27 can regulate hPMSC function. Here, we examined the effects of IL-27 upon adherence, migration, and proliferation as well as the immunomodulatory effects of hPMSCs. The results show that IL-27 receptor α chain (IL-27Rα) is expressed in hPMSCs...
June 14, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28611013/the-effect-of-bone-marrow-mesenchymal-stem-cells-on-the-granulocytic-differentiation-of-hl-60-cells
#16
Hossein Nikkhah, Elham Safarzadeh, Karim Shamsasenjan, Mehdi Yousefi, Parisa Lotfinejad, Mehdi Talebi, Mozhde Mohammadian, Farhoud Golafshan, Ali Akbar Movassaghpour
OBJECTIVE: The mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types. They control the process of haematopoiesis by secreting regulatory cytokines and growth factors and by the expression of important cell adhesion molecules for cell-to-cell interactions. This investigation intended to examine the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on the differentiation of the HL-60 cells according to morphological evaluation, flow cytometry analysis, andgene expression profile...
June 13, 2017: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/28610623/transplantation-of-human-bone-marrow-mesenchymal-stromal-cells-reduces-liver-fibrosis-more-effectively-than-wharton-s-jelly%C3%A2-mesenchymal-stromal-cells
#17
Mathiyazhagan Rengasamy, Gurbind Singh, Noor Atiqah Fakharuzi, Siddikuzzaman, Sudha Balasubramanian, Priyanka Swamynathan, Charan Thej, Gopinath Sasidharan, Pawan Kumar Gupta, Anjan Kumar Das, Ahmad Zuhairi Abd Rahman, Kamal Shaik Fakiruddin, Lim Moon Nian, Zubaidah Zakaria, Anish S Majumdar
BACKGROUND: Mesenchymal stromal cells (MSCs) from various tissues have shown moderate therapeutic efficacy in reversing liver fibrosis in preclinical models. Here, we compared the relative therapeutic potential of pooled, adult human bone marrow (BM)- and neonatal Wharton's jelly (WJ)-derived MSCs to treat CCl4-induced liver fibrosis in rats. METHODS: Sprague-Dawley rats were injected with CCl4 for 8 weeks to induce irreversible liver fibrosis. Ex-vivo expanded, pooled human MSCs obtained from BM and WJ were intravenously administered into rats with liver fibrosis at a dose of 10 × 10(6) cells/animal...
June 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28607949/non-glycanated-decorin-is-a-drug-target-on-human-adipose-stromal-cells
#18
Alexes C Daquinag, Ali Dadbin, Brad Snyder, Xiaoping Wang, Aysegul A Sahin, Naoto T Ueno, Mikhail G Kolonin
Adipose stromal cells (ASCs) have been identified as a mesenchymal cell population recruited from white adipose tissue (WAT) by tumors and supporting cancer progression. We have previously reported the existence of a non-glycanated decorin isoform (ngDCN) marking mouse ASCs. We identified a peptide CSWKYWFGEC that binds to ngDCN and hence can serve as a vehicle for ASC-directed therapy delivery. We used hunter-killer peptides composed of CSWKYWFGEC and a pro-apoptotic moiety to deplete ASCs and suppress growth of mouse tumors...
September 15, 2017: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/28607804/mesenchymal-stromal-cells-role-in-tumor-microenvironment-involvement-of-signaling-pathways
#19
Armel Herve Nwabo Kamdje, Paul Takam Kamga, Richard Tagne Simo, Lorella Vecchio, Paul Faustin Seke Etet, Jean Marc Muller, Giulio Bassi, Erique Lukong, Raghuveera Kumar Goel, Jeremie Mbo Amvene, Mauro Krampera
Mesenchymal stromal cells (MSCs) are adult multipotent stem cells residing as pericytes in various tissues and organs where they can differentiate into specialized cells to replace dying cells and damaged tissues. These cells are commonly found at injury sites and in tumors that are known to behave like " wounds that do not heal." In this article, we discuss the mechanisms of MSCs in migrating, homing, and repairing injured tissues. We also review a number of reports showing that tumor microenvironment triggers plasticity mechanisms in MSCs to induce malignant neoplastic tissue formation, maintenance, and chemoresistance, as well as tumor growth...
May 2017: Cancer Biology & Medicine
https://www.readbyqxmd.com/read/28607396/cxcl12-and-osteopontin-from-bone-marrow-derived-mesenchymal-stromal-cells-improve-muscle-regeneration
#20
Yasushi Maeda, Yasuhiro Yonemochi, Yuki Nakajyo, Hideaki Hidaka, Tokunori Ikeda, Yukio Ando
Muscle satellite cells are essential for muscle regeneration. However, efficient regeneration does not occur without muscle-resident mesenchymal progenitor cells. We show here that bone marrow-derived mesenchymal stromal cells (Bm-MSCs) also facilitate muscle regeneration in Duchenne muscular dystrophy (DMD) model mice. Bm-MSCs transplanted into peritoneal cavities of DMD model mice with severe muscle degeneration strongly suppressed dystrophic pathology and improved death-related symptoms, which resulted in dramatic lifespan extension...
June 12, 2017: Scientific Reports
keyword
keyword
109832
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"