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Mesenchymal stromal cell

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https://www.readbyqxmd.com/read/29334181/donor-variability-in-growth-kinetics-of-healthy-hmscs-using-manual-processing-considerations-for-manufacture-of-cell-therapies
#1
Giulia Detela, Owen W Bain, Hae-Won Kim, David J Williams, Chris Mason, Anthony Mathur, Ivan B Wall
Human mesenchymal stromal cells (hMSCs) are excellent candidates for cell therapy but their expansion to desired clinical quantities can be compromised by ex vivo processing, due to differences between donor material and process variation. The aim of this article was to characterize growth kinetics of healthy baseline "reference" hMSCs using typical manual processing. Bone-marrow derived hMSCs from ten donors were isolated based on plastic adherence, expanded and analyzed for their growth kinetics until passage 4...
January 15, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29333563/aldehyde-dehydrogenase-activity-in-adipose-tissue-isolation-and-gene-expression-profile-of-distinct-sub-population-of-mesenchymal-stromal-cells
#2
Mehdi Najar, Emerence Crompot, Leo A van Grunsven, Laurent Dollé, Laurence Lagneaux
Thanks to their relative abundance and easier collection, adipose tissue (AT) is considered an alternative source for the isolation of mesenchymal stromal cells (MSCs). MSCs have great therapeutic values and are thus under investigations for several clinical indications such as regenerative medicine and immunomodulation. In this work, we aimed to identify, isolate and characterize AT-MSCs based on their aldehyde dehydrogenase (ALDH) activity known to be a classical feature of stem cells. FACS technology allowed to isolate two different populations of AT-MSCs according to their ALDH activity (referred as ALDH+ and ALDH-)...
January 15, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29331956/adenosine-production-by-biomaterial-supported-mesenchymal-stromal-cells-reduces-the-innate-inflammatory-response-in-myocardial-ischemia-reperfusion-injury
#3
Eric Y Shin, Lanfang Wang, Marina Zemskova, Juline Deppen, Kai Xu, Frederick Strobel, Andrés J García, Rabindra Tirouvanziam, Rebecca D Levit
BACKGROUND: During myocardial ischemia/reperfusion (MI/R) injury, there is extensive release of immunogenic metabolites that activate cells of the innate immune system. These include ATP and AMP, which upregulate chemotaxis, migration, and effector function of early infiltrating inflammatory cells. These cells subsequently drive further tissue devitalization. Mesenchymal stromal cells (MSCs) are a potential treatment modality for MI/R because of their powerful anti-inflammatory capabilities; however, the manner in which they regulate the acute inflammatory milieu requires further elucidation...
January 13, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29331291/global-microrna-profiling-in-human-bone-marrow-skeletal-stromal-or-mesenchymal-stem-cells-identified-candidates-for-bone-regeneration
#4
Chi-Chih Chang, Morten T Venø, Li Chen, Nicholas Ditzel, Dang Q S Le, Philipp Dillschneider, Moustapha Kassem, Jørgen Kjems
Bone remodeling and regeneration are highly regulated multistep processes involving posttranscriptional regulation by microRNAs (miRNAs). Here, we performed a global profiling of differentially expressed miRNAs in bone-marrow-derived skeletal cells (BMSCs; also known as stromal or mesenchymal stem cells) during in vitro osteoblast differentiation. We functionally validated the regulatory effects of several miRNAs on osteoblast differentiation and identified 15 miRNAs, most significantly miR-222 and miR-423, as regulators of osteoblastogenesis...
December 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29331104/inhibition-of-mircorna-34a-enhances-survival-of-human-bone-marrow-mesenchymal-stromal-stem-cells-under-oxidative-stress
#5
Yang Liu, Xiaohu Zhang, Jie Chen, Tingyu Li
BACKGROUND Mesenchymal stromal/stem cells (MSCs) are broadly used for many diseases, but the efficacy of MSC engraftment is very low due to low viability and high cell death rate under a stressful microenvironment. The present study aimed to investigate whether microRNA-34a (miR-34a), which is a downstream target of P53, is involved in H2O2-induced MSC cell death. MATERIAL AND METHODS Human bone marrow MSCs (hMSCs) were purchased from Lonza and were cultured as previously described. hMSCs were transfected with miR-34a inhibitor and exposed to H2O2...
January 13, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29327669/advanced-3d-models-culture-to-investigate-mesenchymal-stromal-cells-of-the-human-dental-follicle
#6
Nathalie Steimberg, Francesca Angiero, Davide Farronato, Angiola Berenzi, Gianguido Cossellu, Andrea Ottonello, Darnell Kaigler, Giovanna Mazzoleni
The human dental follicle (hDF) contains the developing tooth and is involved in regulating tooth maturation and eruption. To investigate the mesenchymal stromal cells of the dental follicle, two 3D culture models were used, based on a dynamic bioreactor: the Rotary Cell Culture System (RCCS) and the 3D culture of precursor cells isolated from follicular tissue (hDFCs). The hDFCs were obtained from impacted third molars of 20 patients. Two 3D culture models were tested. In the first model, intact hDF explants were cultured in 3D conditions, preserving the original tissue architecture; they were studied via histomorphological and molecular analyses...
January 12, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29325970/the-role-of-adjuvant-immunomodulatory-agents-for-treatment-of-severe-influenza
#7
REVIEW
David S Hui, Nelson Lee, Paul K Chan, John H Beigel
A severe inflammatory immune response with hypercytokinemia occurs in patients hospitalized with severe influenza, such as avian influenza A(H5N1), A(H7N9), and seasonal A(H1N1)pdm09 virus infections. The role of immunomodulatory therapy is unclear as there have been limited published data based on randomized controlled trials (RCTs). Passive immunotherapy such as convalescent plasma and hyperimmune globulin have some studies demonstrating benefit when administered as an adjunctive therapy for severe influenza...
January 8, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29324934/stem-cells-therapy-the-future-in-the-management-of-systemic-sclerosis-a-case-report
#8
Jung In Song, Silvanie Volz, Maria Eirini Liodaki, Peter Mailänder, Konstantinos Kalousis
OBJECTIVE: Systemic sclerosis (SSc) is a connective tissue disorder of unknown etiology, with heterogeneous clinical manifestations and chronic and often progressive course. The diffuse cutaneous form of SSc (dcSSc) is characterized by thickening of the skin (scleroderma) and distinctive involvement of multiple internal organs. Patients with limited cutaneous SSc (lcSSc) generally have long-standing Raynaud's phenomenon before other manifestations of SSc appear. Over the last decade the Interest of adipose-derived cell therapy in regenerative medicine has increased continuously...
September 2017: Hellenic Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/29323194/human-mesenchymal-stromal-cell-derived-extracellular-vesicles-modify-microglial-response-and-improve-clinical-outcomes-in-experimental-spinal-cord-injury
#9
Katherine A Ruppert, Tin T Nguyen, Karthik S Prabhakara, Naama E Toledano Furman, Amit K Srivastava, Matthew T Harting, Charles S Cox, Scott D Olson
No current clinical intervention can alter the course of acute spinal cord injury (SCI), or appreciably improve neurological outcome. Mesenchymal stromal cells (MSCs) have been shown to modulate the injury sequelae of SCI largely via paracrine effects, although the mechanisms remain incompletely understood. One potential modality is through secretion of extracellular vesicles (EVs). In this study, we investigate whether systemic administration of EVs isolated from human MSCs (MSCEv) has the potential to be efficacious as an alternative to cell-based therapy for SCI...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29322348/optimisation-of-a-potency-assay-for-the-assessment-of-immunomodulative-potential-of-clinical-grade-multipotent-mesenchymal-stromal-cells
#10
Irene Oliver-Vila, Carmen Ramírez-Moncayo, Marta Grau-Vorster, Sílvia Marín-Gallén, Marta Caminal, Joaquim Vives
Clinical use of multipotent Mesenchymal Stromal Cell (MSC)-based medicinal products requires their production in compliance with Good Manufacturing Practices, thus ensuring that the final drug product meets specifications consistently from batch to batch in terms of cell viability, identity, purity and potency. Potency relates to the efficacy of the medicine in its target clinical indication, so adequate release tests need to be defined and validated as quality controls. Herein we report the design and optimisation of parameters affecting the performance of an in vitro cell-based assay for assessing immunomodulatory potential of clinical grade MSC for human use, based on their capacity to inhibit proliferation of T lymphocytes under strong polyclonal stimuli...
January 10, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29321063/first-clinical-case-report-of-local-microinjection-of-autologous-fat-and-adipose-derived-stromal-vascular-fraction-for-perianal-fistula-in-crohn-s-disease
#11
Cécile Philandrianos, Mélanie Serrero, Fanny Grimaud, Jérémy Magalon, Carine Visée, Mélanie Velier, Pauline Francois, Pierre Orsoni, Guy Magalon, Jean-Charles Grimaud, Ariadne Desjeux, Julie Véran, Florence Sabatier
Mesenchymal stem cell therapy is a promising treatment for perianal Crohn's fistulas refractory to conventional therapy, which are an extremely morbid complication and a true therapeutic challenge. Autologous adipose-derived stromal vascular fraction (ADSVF) is an easily accessible source of cells with angiogenic, anti-inflammatory, immunomodulatory, and regenerative properties. Here, we describe a case involving a patient with severe perianal Crohn's fistulas refractory to the best medical and surgical practices who received local treatment with ADSVF and microfat...
January 10, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29321040/automated-image-analysis-detects-aging-in-clinical-grade-mesenchymal-stromal-cell-cultures
#12
S Oja, P Komulainen, A Penttilä, J Nystedt, M Korhonen
BACKGROUND: Senescent cells are undesirable in cell therapy products due to reduced therapeutic activity and risk of aberrant cellular effects, and methods for assessing senescence are needed. Early-passage mesenchymal stromal cells (MSCs) are known to be small and spindle-shaped but become enlarged upon cell aging. Indeed, cell morphology is routinely evaluated during MSC production using subjective methods. We have therefore explored the possibility of utilizing automated imaging-based analysis of cell morphology in clinical cell manufacturing...
January 10, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29319804/effects-of-agricultural-organic-dusts-on-human-lung-resident-mesenchymal-stem-stromal-cell-function
#13
Tara M Nordgren, Kristina L Bailey, Art J Heires, Dawn Katafiasz, Debra J Romberger
Agricultural organic dust exposures trigger harmful airway inflammation, and workers experiencing repetitive dust exposures are at increased risk for lung disease. Mesenchymal stem/stromal cells (MSC) regulate wound repair processes in the lung, and may contribute to either pro-resolution or pro-fibrotic lung responses. It is unknown how organic dust exposures alter lung-resident MSC activation and pro-inflammatory versus pro-repair programs in the lung. To address this gap in knowledge, we isolated human lung-resident MSC from lung tissue...
January 8, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29319174/chronic-high-glucose-and-insulin-stimulate-bone-marrow-stromal-cells-adipogenic-differentiation-in-young-spontaneously-hypertensive-rats
#14
Antonio Hernandes Chaves Neto, Victor Gustavo Balera Brito, Thamine Landim de Barros, Caril Constante Ferreira do Amaral, Dóris Hissako Sumida, Sandra Helena Penha Oliveira
We evaluated whether genetic predisposition is sufficient to induce changes due to chronic high glucose (HG; 25 mmol/L) in the presence or absence of insulin (HGI; 10 µg/mL) on osteogenic differentiation and markers in bone-marrow mesenchymal stem cells (BMSCs) from young Wistar (WBMSCs) and spontaneous hypertensive rats (SBMSCs) without hypertension. HG suppressed osteogenic differentiation in both the strains, observed by mineralization inhibition and decreased levels of the osteogenic markers Runx2, osterix, osteopontin, and bone sialoprotein, compared to osteogenic medium (OM) cells...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29314362/a-small-population-of-resident-limb-bud-mesenchymal-cells-expresses-few-msc-associated-markers-but-the-expression-of-these-markers-is-increased-immediately-after-cell-culture
#15
Jessica Cristina Marín-Llera, Jesús Chimal-Monroy
Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors or multipotent mesenchymal stromal cells (MSC). Derived-MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Here, comparing non-cultured versus cultured mesenchymal limb bud cells, we determined the expression of MSC-associated markers, the in vitro differentiation capacity and their gene expression profile...
January 4, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29313232/activation-of-nk-cells-in-mixed-cultures-of-wharton-s-jelly-mesenchymal-stromal-cells-and-peripheral-blood-lymphocytes
#16
E V Svirshchevskaya, A M Poltavtsev, G Zh Os'mak, R A Poltavtseva
Mesenchymal stromal cells possess immunosuppressive properties that might be used for the therapy of inflammatory diseases of various geneses. The effects of mesenchymal stromal cells depend on their lifetime in the recipient tissues. During heterologous transplantation, mesenchymal stromal cells are eliminated by NK cells. We studied NK cell formation in mixed cultures of Wharton's jelly mesenchymal stromal cells and peripheral blood lymphocytes from an autologous donor. Lymphocytes were activated by a mitogen or IL-2...
January 8, 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29311424/jaw-bones-regeneration-using-mesenchymal-stem-cells-a-single-center-experience
#17
Walter Colangeli, Umberto Riccelli, Amerigo Giudice, Ida Barca, Davide Caruso, Daniela Novembre, Claudio Tortosa, Raffaella Cordaro, Maria Giulia Cristofaro
PURPOSE: Mesenchymal stem cells (MSC), which are multipotent stromal cells, are considered to be a promising resource in tissue engineering and tissue regeneration. MSCs have been used to generate new maxillary bone with clinically successful results. The aim of this study was to determine the role of MSC in bone regeneration procedures in patients with benign maxillary lesions. METHODS: A study was conducted on five patients treated for maxillary bone defects resulting from biopsy of benign lesions at the University Hospital of Magna Græcia, Catanzaro, Italy from January 2015 to October 2016...
December 21, 2017: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/29306567/platelet-rich-plasma-derived-scaffolds-increase-the-benefit-of-delayed-mesenchymal-stromal-cell-therapy-after-severe-traumatic-brain-injury
#18
Celia Bonilla Horcajo, Mercedes Zurita Castillo, Jesús Vaquero Crespo
BACKGROUND: Cell therapy using mesenchymal stromal cells (MSCs) offers new perspectives in the treatment of traumatic brain injury (TBI). The aim of the present study was to assess the impact of platelet-rich plasma scaffolds (PRPS) as support of MSCs in a delayed phase after severe TBI in rats. METHODS: TBI was produced by weight-drop impact to the right cerebral hemisphere. Two months after TBI, four experimental groups were established; saline, PRPS, MSCs in saline, or MSCs in PRPS was transplanted into the area of brain lesion through a small hole...
January 3, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29305674/comparable-osteogenic-capacity-of-mesenchymal-stem-or-stromal-cells-derived-from-human-amnion-membrane-and-bone-marrow
#19
Mehran Ghasemzadeh, Ehteramolsadat Hosseini, Mohammadhossein Ahmadi, Maedeh Kamalizad, Naser Amirizadeh
So far, substantial attentions have been attracted to the application of mesenchymal stem or stromal cells (MSCs) in different therapeutic approaches. Although human bone marrow is commonly considered as a major source for MSCs, having an invasive collection method, ethical consideration and donor availability create a challenge for scientists, leading them to explore better alternative sources for MSCs. The study presented here aimed to characterize and compare osteogenic capacity of MSCs obtained from the amnion membrane (AM) with those originated from BM...
January 5, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29305587/consensus-molecular-subtypes-of-colorectal-cancer-are-recapitulated-in-in-vitro-and-in-vivo-models
#20
Janneke F Linnekamp, Sander R van Hooff, Pramudita R Prasetyanti, Raju Kandimalla, Joyce Y Buikhuisen, Evelyn Fessler, Prashanthi Ramesh, Kelly A S T Lee, Grehor G W Bochove, Johan H de Jong, Kate Cameron, Ronald van Leersum, Hans M Rodermond, Marek Franitza, Peter Nürnberg, Laura R Mangiapane, Xin Wang, Hans Clevers, Louis Vermeulen, Giorgio Stassi, Jan Paul Medema
Colorectal cancer (CRC) is a highly heterogeneous disease both from a molecular and clinical perspective. Several distinct molecular entities, such as microsatellite instability (MSI), have been defined that make up biologically distinct subgroups with their own clinical course. Recent data indicated that CRC can be best segregated into four groups called consensus molecular subtypes (CMS1-4), each of which has a unique biology and gene expression pattern. In order to develop improved, subtype-specific therapies and to gain insight into the molecular wiring and origin of these subtypes, reliable models are needed...
January 5, 2018: Cell Death and Differentiation
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