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https://www.readbyqxmd.com/read/28109104/-clinical-significance-of-peripheral-blood-neutrophil-lymphocyte-ratio-and-platelet-lymphocyte-ratio-in-patients-with-asthma
#1
Guang Shi, Jun-Wei Zhao, Liang Ming
OBJECTIVE: To investigate the changes of peripheral blood neutrophil-lymphocyte ratio (NLR) and platelet- lymphocyte ratio (PLR) in patients in acute stage of bronchial asthma and their clinical significance. METHODS: A total of 262 patients with acute asthma, including 97 critical and 175 non-critical patients, were recruited from our hospital between January, 2013 and May, 2016, with 130 healthy individuals as the control group. The absolute neutrophil count, absolute lymphocyte count, platelet, NLR and PLR were compared among different groups, and their diagnostic values were evaluated using the area under the receiver-operating characteristic (ROC) curve...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28109063/chemical-variability-antioxidant-and-antifungal-activities-of-essential-oils-and-hydrosol-extract-of-calendula-arvensis-l-from-western-algeria
#2
Rania Belabbes, Mohammed El Amine Dib, Nassim Djabou, Faiza Ilias, Boufeldja Tabti, Jean Costa, Alain Muselli
The chemical composition of the essential oils and hydrosol extract from aerial parts of Calendula arvensis L. was investigated using GC-FID and GC/MS. Intra-species variations of the chemical compositions of essential oils from 18 Algerian sample locations were investigated using statistical analysis. Chemical analysis allowed the identification of fifty-three compounds amounting to 92.3-98.5% with yields varied of 0.09-0.36% and the main compounds were zingiberenol 1 (8.7-29.8%), eremoligenol (4.2-12.5%), β-curcumene (2...
January 21, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28109018/gonad-histology-and-serum-11-kt-profile-during-the-annual-reproductive-cycle-in-sterlet-sturgeon-adult-males-acipenser-ruthenus
#3
A Golpour, C Broquard, S Milla, H Dadras, A R Baloch, T Saito, M Pšenička
The aim of this study was to assess monthly testicular development in the cultured breeding stock of sterlet, Acipenser ruthenus, using histological and serum sex steroid changes. Testicular development in the adult male was examined monthly and showed four distinct phases including resting, pre-spawning, spawning and post-spawning. Also, seasonal changes of the testes were described according to its variations in gonadosomatic index (GSI) during different phases of testicular development. Using histology, we identified continuous spermatogenesis and asynchronous gonad development pattern in the testes of male sterlet, which shows that regulation of annual gonadal cycle is influenced by season...
January 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28109005/the-mboat7-variant-rs641738-increases-inflammation-and-fibrosis-in-chronic-hepatitis-b
#4
Khaled Thabet, Henry Lik Yuen Chan, Salvatore Petta, Alessandra Mangia, Thomas Berg, Andre Boonstra, Willem P Brouwer, Maria Lorena Abate, Vincent Wai-Sun Wong, Maiiada Nazmy, Janett Fischer, Christopher Liddle, Jacob George, Mohammed Eslam
Chronic hepatitis B (CHB) is characterized by hepatic inflammation that promotes progression to cirrhosis and predisposes to the development of hepatocellular carcinoma (HCC). Subtle inter-individual genetic variation, viral and environmental factors interact to determine the disease progression between individuals. Recently, the rs641738 Membrane Bound O-Acyltransferase Domain Containing 7 (MBOAT7) polymorphism was demonstrated to influence histological liver damage in alcoholic liver disease, nonalcoholic fatty liver disease and hepatitis C, but no data are available for CHB...
January 20, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28108977/a-locus-coeruleus-norepinephrine-account-of-individual-differences-in-working-memory-capacity-and-attention-control
#5
Nash Unsworth, Matthew K Robison
Studies examining individual differences in working memory capacity (WMC) have suggested that low WMC individuals have particular deficits in attention control processes compared to high WMC individuals. In the current article we suggest that part of the WMC-attention control relation is due to variation in the functioning of the locus coeruleus-norepinephrine system (LC-NE). Specifically, we suggest that because of dysregulation of LC-NE functioning, the fronto-parietal control network for low WMC individuals is only weakly activated, resulting in greater default-mode network activity (and greater mind-wandering) for low WMC individuals compared to high WMC individuals...
January 20, 2017: Psychonomic Bulletin & Review
https://www.readbyqxmd.com/read/28108957/effect-of-temperature-on-surface-characteristics-of-nitrogen-ion-implanted-biocompatible-titanium
#6
Hossein Aghajani, Mohsen Sadeghpour Motlagh
It has been established that nitrogen implantation can modify metals surface properties such as corrosion, wear, friction, etc. Recent studies have shown that nitrogen implantation into titanium and its alloys leads to the formation of titanium nitride, which has interesting biological, chemical, and mechanical properties. In the present investigation, commercially pure biocompatible titanium implanted by nitrogen. Implantation of ions performed at 80 keV and a dose of 2.1 × 10(18) ions/cm(2) at four different temperatures of 300, 473, 673 and 873 k...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108874/surviving-in-isolation-genetic-variation-bottlenecks-and-reproductive-strategies-in-the-canarian-endemic-limonium-macrophyllum-plumbaginaceae
#7
Ares Jiménez, Barbara Weigelt, Arnoldo Santos-Guerra, Juli Caujapé-Castells, José María Fernández-Palacios, Elena Conti
Oceanic archipelagos are typically rich in endemic taxa, because they offer ideal conditions for diversification and speciation in isolation. One of the most remarkable evolutionary radiations on the Canary Islands comprises the 16 species included in Limonium subsection Nobiles, all of which are subject to diverse threats, and legally protected. Since many of them are single-island endemics limited to one or a few populations, there exists a risk that a loss of genetic variation might limit their long-term survival...
January 20, 2017: Genetica
https://www.readbyqxmd.com/read/28108840/differences-in-monoterpene-biosynthesis-and-accumulation-in-pistacia-palaestina-leaves-and-aphid-induced-galls
#8
Karin Rand, Einat Bar, Matan Ben Ari, Rachel Davidovich-Rikanati, Natalia Dudareva, Moshe Inbar, Efraim Lewinsohn
Certain insect species can induce gall formation on numerous plants species. Although the mechanism of gall development is largely unknown, it is clear that insects manipulate their hosts' anatomy, physiology, and chemistry for their own benefit. It is well known that insect-induced galls often contain vast amounts of plant defensive compounds as compared to non-colonized tissues, but it is not clear if defensive compounds can be produced in situ in the galled tissues. To answer this question, we analyzed terpene accumulation patterns and possible independent biosynthetic potential of galls induced by the aphid Baizongia pistaciae L...
January 20, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28108754/using-fluorescence-immunochromatographic-test-strips-based-on-quantum-dots-for-the-rapid-and-sensitive-determination-of-microcystin-lr
#9
Jiadi Sun, Ying Li, Fuwei Pi, Jian Ji, Yinzhi Zhang, Xiulan Sun
A novel immunosensor for the detection of microcystin-LR (MC-LR) was constructed with use of immunochromatographic test strips (ICTS). Quantum dots were chosen to be the fluorescent labels for the immune sensor in ICTS because of their excellent optical and electronic properties with a relatively narrow emission spectrum. The detection sensitivity of the ICTS was related to the concentration of the fluorescent probe and the amount of the MC-LR standards. Under optimal conditions, with MC-LR as the target, the ICTS sensor had a linear range from 0...
January 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28108698/genome-wide-analysis-of-fatty-acid-desaturation-and-its-response-to-temperature
#10
Guillaume Menard, Jose Martin Moreno, Fiona Bryant, Olaya Munoz-Azcarate, Amélie A Kelly, Keywan Hassani-Pak, Smita Kurup, Peter J Eastmond
Plants modify the polyunsaturated fatty acid content of their membrane and storage lipids in order to adapt to changes in temperature. In developing seeds this response is largely controlled by the activities of the microsomal ω-6 and ω-3 fatty acid desaturases, FAD2 and FAD3. Although temperature-regulation of desaturation has been studied at the molecular and biochemical level, the genetic control of this trait is poorly understood. Here we have characterised the response of Arabidopsis thaliana seed lipids to variation in ambient temperature and found that heat inhibits both ω-6 and ω-3 desaturation in phosphatidylcholine, leading to a proportional change in triacylglycerol composition...
January 20, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28108668/ultraviolet-and-yellow-reflectance-but-not-fluorescence-is-important-for-visual-discrimination-of-conspecifics-by-heliconius-erato
#11
Susan D Finkbeiner, Dmitry A Fishman, Daniel Osorio, Adriana D Briscoe
Toxic Heliconius butterflies have yellow hindwing bars that - unlike their closest relatives - reflect ultraviolet (UV) and long wavelength light, and also fluoresce. The pigment in the yellow scales is 3-hydroxy-DL-kynurenine (3-OHK), found also in the hair and scales of a variety of animals. In other butterflies like pierids with color schemes characterized by independent sources of variation in UV and human-visible yellow/orange, behavioral experiments have generally implicated the UV component as most relevant to mate choice...
January 20, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28108655/the-novel-lysine-specific-methyltransferase-mettl21b-affects-mrna-translation-through-inducible-and-dynamic-methylation-of-lys-165-in-human-eukaryotic-elongation-factor-1-alpha-eef1a
#12
Jędrzej Małecki, Vinay Kumar Aileni, Angela Y Y Ho, Juliane Schwarz, Anders Moen, Vigdis Sørensen, Benedikt S Nilges, Magnus E Jakobsson, Sebastian A Leidel, Pål Ø Falnes
Lysine methylation is abundant on histone proteins, representing a dynamic regulator of chromatin state and gene activity, but is also frequent on many non-histone proteins, including eukaryotic elongation factor 1 alpha (eEF1A). However, the functional significance of eEF1A methylation remains obscure and it has remained unclear whether eEF1A methylation is dynamic and subject to active regulation. We here demonstrate, using a wide range of in vitro and in vivo approaches, that the previously uncharacterized human methyltransferase METTL21B specifically targets Lys-165 in eEF1A in an aminoacyl-tRNA- and GTP-dependent manner...
January 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28108592/social-class-variation-the-effect-of-the-economic-recession-and-childhood-obesity-at-3-years-of-age-in-ireland
#13
Samira Barbara Jabakhanji, Milena Pavlova, Wim Groot, Fiona Boland, Regien Biesma
BACKGROUND: Many studies have reported a social class gradient within overweight prevalence (1-4). Additionally, cross-country comparisons report high overweight trends and a change in food consumption patterns in countries affected by an economic crisis (5-11). The aim of this study was to assess the association between social class and recession on obesity levels in 3-year-old Irish children. METHODS: The population-based infant cohort of the national Growing Up in Ireland (GUI) study was used...
January 19, 2017: European Journal of Public Health
https://www.readbyqxmd.com/read/28108578/the-activated-clotting-time-in-cardiac-surgery-should-celite-or-kaolin-be-used
#14
Adrianus J De Vries, Annemieke Oude Lansink-Hartgring, Freek-Jan Fernhout, Rolf C G Huet, Edwin R van den Heuvel
OBJECTIVES: Both kaolin- and Celite-activated clotting times (ACT) are used to guide anticoagulation during cardiopulmonary bypass. It is unknown whether these methods lead to similar management procedures for anticoagulation in patients and are thus interchangeable in terms of bias, precision and variability. METHODS: We randomized 97 patients undergoing coronary artery bypass grafting or aortic valve replacement to either kaolin- or Celite-guided anticoagulation...
January 20, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28108445/multi-rate-poisson-tree-processes-for-single-locus-species-delimitation-under-maximum-likelihood-and-markov-chain-monte-carlo
#15
P Kapli, S Lutteropp, J Zhang, K Kobert, P Pavlidis, A Stamatakis, T Flouri
MOTIVATION: In recent years, molecular species delimitation has become a routine approach for quantifying and classifying biodiversity. Barcoding methods are of particular importance in large-scale surveys as they promote fast species discovery and biodiversity estimates. Among those, distance-based methods are the most common choice as they scale well with large datasets; however, they are sensitive to similarity threshold parameters and they ignore evolutionary relationships. The recently introduced "Poisson Tree Processes" (PTP) method is a phylogeny-aware approach that does not rely on such thresholds...
January 19, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28108432/living-on-the-edge-conservation-genetics-of-seven-thermophilous-plant-species-in-a-high-arctic-archipelago
#16
Siri Birkeland, Idunn Elisabeth Borgen Skjetne, Anne Krag Brysting, Reidar Elven, Inger Greve Alsos
Small, isolated, and/or peripheral populations are expected to harbour low levels of genetic variation and may therefore have reduced adaptability to environmental change, including climate warming. In the Arctic, global warming has already caused vegetation change across the region and is acting as a significant stressor on Arctic biodiversity. Many of the rare plants in the Arctic are relicts from early Holocene warm periods, but their ability to benefit from the current warming is dependent on the viability of their populations...
January 20, 2017: AoB Plants
https://www.readbyqxmd.com/read/28108412/deciphering-the-mechanistic-insight-into-the-stoichiometric-ratio-dependent-behavior-of-cu-ii-on-bsa-fibrillation
#17
Ajeet Singh, Poulami Datta, Lalit M Pandey
Various metal ions are recently implicated in protein aggregates and are associated with numerous neurodegenerative diseases. In the present work, we have scrutinized the effect of stoichiometric variation of Cu(II) on BSA fibrillation at physiological pH 7.4 through Thioflavin-T dye binding study, residual protein concentration, Fourier Transform Infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Isothermal Titration Calorimetry (ITC). Fibrillation kinetics was studied through ThT fluorescence, which illustrated dependency on stoichiometric ratio of Cu(II)...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108406/berry-s-ligament-and-the-inferior-thyroid-artery-as-reliable-anatomical-landmarks-for-the-recurrent-laryngeal-nerve-a-fresh-cadaveric-study-of-the-cervical-spine-the-recurrent-laryngeal-nerve-rln-relevant-to-spine
#18
Ali Rajabian, Michael Walsh, Nasir A Quraishi
BACKGROUND CONTEXT: Whilst most cadaveric studies of the RLN have focused on course variations, they have usually been done on preserved (fixed and embalmed) cadavers which renders the RLN immobile and of less surgical landmark value. PURPOSE: Our aim was to perform a thorough exposure in fresh cadavers with the intention of investigating inferior thyroid artery and Berry's ligament as reliable landmarks for the identification of the RLN in anterior cervical spine surgery...
January 17, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28108378/the-pressure-induced-deformation-response-of-the-human-lamina-cribrosa-analysis-of-regional-variations
#19
Dan E Midgett, Mary E Pease, Joan L Jefferys, Mohak Patel, Christian Franck, Harry A Quigley, Thao D Nguyen
: The objective of this study was to measure the pressure-induced deformation response of the human lamina cribrosa (LC) and analyze for variations with age and anatomical region. The posterior scleral cup of 8 eyes from 6 human donors was mounted onto a custom inflation chamber. A laser-scanning microscope was used for second harmonic generation (SHG), imaging the collagen structure in the posterior volume of the LC at pressures from 5 mmHg to 45 mmHg. The SHG volumes were analyzed by the Fast-Fourier Iterative Digital Volume Correlation (DVC) algorithm for the three dimensional (3D) displacement field...
January 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28108354/abcs-of-dna-aptamer-and-related-assay-development
#20
REVIEW
Tarun Kumar Sharma, John G Bruno, Abhijeet Dhiman
This review is intended to guide the novice in aptamer research and development to understand virtually all of the aptamer development options and currently available assay modalities. Aptamer development topics range from discussions of basic and advanced versions of Systematic Evolution of Ligands by EXponential Enrichment (SELEX) and SELEX variations involving incorporation of exotic unnatural nucleotides to expand library diversity for even greater aptamer affinity and specificity to improved next generation methods of DNA sequencing, screening and tracking aptamer development throughout the SELEX process and characterization of lead aptamer candidates...
January 17, 2017: Biotechnology Advances
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