keyword
MENU ▼
Read by QxMD icon Read
search

KASP

keyword
https://www.readbyqxmd.com/read/29224884/a-genome-wide-association-study-for-left-sided-displacement-of-the-abomasum-using-a-high-density-single-nucleotide-polymorphism-array
#1
S Lehner, I Zerbin, K Doll, J Rehage, O Distl
Left-sided displacement of the abomasum (LDA) is a frequent disease in dairy cattle causing significant financial losses for dairy farmers. Heritability (h2) of this complex disease was estimated at up to 0.5 in German Holstein (GH) cattle. Using the Bovine High Density BeadChip (Illumina Inc., San Diego, CA) comprising 588,753 single nucleotide polymorphisms (SNP) after quality control for 126 LDA cases and 280 population-based controls, we used a mixed linear model analysis in a genome-wide association study (GWAS)...
December 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29224171/genetic-mapping-and-validation-of-the-loci-controlling-7s-%C3%AE-and-11s-a-type-storage-protein-subunits-in-soybean-glycine-max-l-merr
#2
Jeffrey D Boehm, Vi Nguyen, Rebecca M Tashiro, Dale Anderson, Chun Shi, Xiaoguang Wu, Lorna Woodrow, Kangfu Yu, Yuhai Cui, Zenglu Li
Four soybean storage protein subunit QTLs were mapped using bulked segregant analysis and an F2 population, which were validated with an F5 RIL population. The storage protein globulins β-conglycinin (7S subunit) and glycinin (11S subunits) can affect the quantity and quality of proteins found in soybean seeds and account for more than 70% of the total soybean protein. Manipulating the storage protein subunits to enhance soymeal nutrition and for desirable tofu manufacturing characteristics are two end-use quality goals in soybean breeding programs...
December 9, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29222636/identification-introgression-and-molecular-marker-genetic-analysis-and-selection-of-a-highly-effective-novel-oat-crown-rust-resistance-from-diploid-oat-avena-strigosa
#3
Howard W Rines, Marisa E Miller, Martin Carson, Shiaoman Chao, Tyler Tiede, Jochum Wiersma, Shahryar F Kianian
Oat crown rust is one of the most damaging diseases of oat. We identified a new source of resistance and developed KASP and TaqMan markers for selection in breeding programs. A new highly effective resistance to oat crown rust (Puccinia coronata f. sp. avenae) was identified in the diploid oat Avena strigosa PI 258731 and introgressed into hexaploid cultivated oat. Young plants with this resistance show moderate susceptibility, whereas older plant tissues and adult plants are resistant with no virulent isolates encountered in over 8 years of testing...
December 6, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29163598/an-rna-sequencing-transcriptome-analysis-of-grasspea-lathyrus-sativus-l-and-development-of-ssr-and-kasp-markers
#4
Xiaopeng Hao, Tao Yang, Rong Liu, Jinguo Hu, Yang Yao, Marina Burlyaeva, Yan Wang, Guixing Ren, Hongyan Zhang, Dong Wang, Jianwu Chang, Xuxiao Zong
Grasspea (Lathyrus sativus L., 2n = 14) has great agronomic potential because of its ability to survive under extreme conditions, such as drought and flood. However, this legume is less investigated because of its sparse genomic resources and very slow breeding process. In this study, 570 million quality-filtered and trimmed cDNA sequence reads with total length of over 82 billion bp were obtained using the Illumina NextSeq(TM) 500 platform. Approximately two million contigs and 142,053 transcripts were assembled from our RNA-Seq data, which resulted in 27,431 unigenes with an average length of 1,250 bp and maximum length of 48,515 bp...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29118424/methods-for-scarless-selection-free-generation-of-human-cells-and-allele-specific-functional-analysis-of-disease-associated-snps-and-variants-of-uncertain-significance
#5
Nicole B Coggins, Jacob Stultz, Henriette O'Geen, Luis G Carvajal-Carmona, David J Segal
With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public health. Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotyping pipeline utilizing KASP (Kompetitive Allele-Specific PCR) genotyping technology to create scarless isogenic cell models of cancer variants in ~1 month. We successfully modeled two novel variants previously identified by our lab in the PALB2 gene in HEK239 cells, resulting in isogenic cells representing all three genotypes for both variants...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29095103/distinctiveness-of-the-roma-population-within-cyp2b6-worldwide-variation
#6
Željka Tomas, Antonija Kuhanec, Tatjana Škarić-Jurić, Matea Zajc Petranović, Nina Smolej Narančić, Branka Janićijević, Marijana Peričić Salihović
AIM: To determine variation of CYP2B6 gene within the genetically specific Croatian Roma (Gypsy) population originating from India and to examine it in the worldwide perspective. MATERIALS & METHODS: Seven SNP loci (rs12721655, rs2279343, rs28399499, rs34097093, rs3745274, rs7260329 and rs8192709) were genotyped in 439 subjects using Kompetitive Allele Specific PCR (KASP) method. RESULTS: The Croatian Roma took an outlying position in CYP2B6 variation from the worldwide perspective mainly due to their exceptionally high minor allele frequency (MAF) for rs8192709 (12...
November 2, 2017: Pharmacogenomics
https://www.readbyqxmd.com/read/28965125/rapid-identification-of-an-adult-plant-stripe-rust-resistance-gene-in-hexaploid-wheat-by-high-throughput-snp-array-genotyping-of-pooled-extremes
#7
Jianhui Wu, Shengjie Liu, Qilin Wang, Qingdong Zeng, Jingmei Mu, Shuo Huang, Shizhou Yu, Dejun Han, Zhensheng Kang
High-throughput SNP array analysis of pooled extreme phenotypes in a segregating population by KASP marker genotyping permitted rapid, cost-effective location of a stripe rust resistance QTL in wheat. German wheat cultivar "Friedrichswerther" has exhibited high levels of adult plant resistance (APR) to stripe rust in field environments for many years. F2:3 lines and F6 recombinant inbred line (RILs) populations derived from a cross between Friedrichswerther and susceptible landrace Mingxian 169 were evaluated in the field in 2013, 2016 and 2017...
September 30, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28955358/association-mapping-of-diastatic-power-in-uk-winter-and-spring-barley-by-exome-sequencing-of-phenotypically-contrasting-variety-sets
#8
Mark E Looseley, Micha Bayer, Hazel Bull, Luke Ramsay, William Thomas, Allan Booth, Carla De La Fuente Canto, Jenny Morris, Pete E Hedley, Joanne Russell
Diastatic Power (DP) is an important quality trait for malt used in adjunct brewing and distilling. Substantial genetic variation for DP exists within UK elite barley cultivars, but breeding progress has been slow due to the limited demand, compared to the overall barley market, and difficulties in assessing DP. Estimates of DP (taken from recommended and national list trials between 1994 and 2012) from a collection of UK elite winter and spring varieties were used to identify contrasting sets of high and low DP varieties...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28952802/novel-tenascin-c-haplotype-modifies-the-risk-for-a-failure-to-heal-after-rotator-cuff-repair
#9
Rainer Kluger, Klaus R Huber, Philipp G Seely, Christian E Berger, Florian Frommlet
BACKGROUND: Several single-nucleotide polymorphisms (SNPs) in the TNC gene have recently been found to be associated with degenerative rotator cuff tears. HYPOTHESIS: Exonic SNPs in the TNC gene are related to the risk for a failure to heal after rotator cuff repair. STUDY DESIGN: Case-control study; Level of evidence, 3. METHODS: A total of 302 patients from the Vienna area and European Caucasian ancestry underwent mini-open rotator cuff repair for a full-thickness superior or posterosuperior tear and were assessed for the integrity of the repair 1 year postoperatively with a real-time 7...
November 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28951075/first-episode-psychosis-patients-display-increased-plasma-il-18-that-correlates-with-cognitive-dysfunction
#10
Funda Orhan, Helena Fatouros-Bergman, Lilly Schwieler, Simon Cervenka, Lena Flyckt, Carl M Sellgren, Göran Engberg, Sophie Erhardt
No abstract text is available yet for this article.
September 23, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28913866/conversion-of-array-based-single-nucleotide-polymorphic-markers-for-use-in-targeted-genotyping-by-sequencing-in-hexaploid-wheat-triticum-aestivum
#11
Amanda J Burridge, Paul A Wilkinson, Mark O Winfield, Gary L A Barker, Alexandra M Allen, Jane A Coghill, Christy Waterfall, Keith J Edwards
Wheat breeders and academics alike use Single Nucleotide Polymorphisms (SNPs) as molecular markers to characterise regions of interest within the hexaploid wheat genome. A number of SNP-based genotyping platforms are available and their utility depends upon factors such as the available technologies, number of data points required, budgets and the technical expertise required. Unfortunately, markers can rarely be exchanged between existing and newly developed platforms, meaning that previously generated data cannot be compared, or combined, with more recently generated datasets...
September 15, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28892034/shifting-zebrafish-lethal-skeletal-mutant-penetrance-by-progeny-testing
#12
Elliott P Brooks, James T Nichols
Zebrafish mutant phenotypes are often incompletely penetrant, only manifesting in some mutants. Interesting phenotypes that inconsistently appear can be difficult to study, and can lead to confounding results. The protocol described here is a straightforward breeding paradigm to increase and decrease penetrance in lethal zebrafish skeletal mutants. Because lethal mutants cannot be selectively bred directly, the classic selective breeding strategy of progeny testing is employed. This method also includes protocols for Kompetitive Allele Specific PCR (KASP) genotyping zebrafish and staining larval zebrafish cartilage and bone...
September 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28855282/discovery-of-a-novel-stem-rust-resistance-allele-in-durum-wheat-that-exhibits-differential-reactions-to-ug99-isolates
#13
Jayaveeramuthu Nirmala, Jyoti Saini, Maria Newcomb, Pablo Olivera, Sam Gale, Daryl Klindworth, Elias Elias, Luther Talbert, Shiaoman Chao, Justin Faris, Steven Xu, Yue Jin, Matthew N Rouse
Wheat stem rust, caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn, can incur yield losses in susceptible cultivars of durum wheat, Triticum turgidum ssp. durum (Desf.) Husnot. Although several durum cultivars possess the stem rust resistance gene Sr13, additional genes in durum wheat effective against emerging virulent races have not been described. Durum line 8155-B1 confers resistance against the P. graminis f. sp. tritici race TTKST, the variant race of the Ug99 race group with additional virulence to wheat stem rust resistance gene Sr24 However, 8155-B1 does not confer resistance to the first-described race in the Ug99 race group: TTKSK...
October 5, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28832276/combining-snp-genotyping-array-with-bulked-segregant-analysis-to-map-a-gene-controlling-adult-plant-resistance-to-stripe-rust-in-wheat-line-03031-1-5-h62
#14
Jianhui Wu, Qilin Wang, Liangsheng Xu, Xianming Chen, Bei Li, Jingmei Mu, Qingdong Zeng, Lili Huang, Dejun Han, Zhensheng Kang
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Growing resistant cultivars is considered the best approach to manage this disease. In order to identify the resistance gene(s) in wheat line 03031-1-5 H62, which displayed high resistance to stripe rust at adult plant stage, a cross was made between 03031-1-5 H62 and susceptible cultivar Avocet S. The mapping population was tested with Chinese Pst race CYR32 through artificial inoculation in a field in Yangling, Shaanxi Province and in Tianshui, Gansu Province under natural infection...
August 23, 2017: Phytopathology
https://www.readbyqxmd.com/read/28760136/development-of-a-high-density-linkage-map-and-mapping-of-the-three-pistil-gene-pis1-in-wheat-using-gbs-markers
#15
Zaijun Yang, Zhenyong Chen, Zhengsong Peng, Yan Yu, Mingli Liao, Shuhong Wei
BACKGROUND: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F2 mapping population (CM28 × CM28TP) was used to construct a genetic map that is of significant value...
July 31, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28716422/development-of-imidazolinone-herbicide-tolerant-borage-borago-officinalis-l
#16
Dongyan Song, Guohai Wu, Patricia Vrinten, Xiao Qiu
Borage (Borago officinalis) is an annual herb that produces a high level of gamma-linolenic acid (GLA) in its seed oil. Due to the recognized health benefits of GLA, borage is now commercially cultivated worldwide. However, an herbicide-tolerant variety for effective weed management has not yet been developed. Here we report the generation and characterization of ethyl methanesulfonate (EMS) induced borage mutant lines tolerant to the herbicide imidazolinone. An EMS-mutagenized borage population was generated by using a series of concentrations of EMS to treat mature borage seeds...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28709693/squamous-proliferations-on-the-legs-of-women-qualitative-examination-of-histopathology-tp53-sequencing-and-implications-for-diagnosis-in-a-series-of-30-cases
#17
Christine J Ko, Earl J Glusac, Jennifer M McNiff, Nemanja Rodic, David J Leffell
BACKGROUND: Women with multiple squamous cell carcinomas (SCCs) of the legs have a striking clinical phenotype. Numerous tumors can develop in a short period of time. OBJECTIVE: Because histopathologic findings can vary in women with multiple SCC lesions, from keratoacanthoma-like to well-differentiated SCC, we hypothesized that TP53 variants might shed light on the appropriate classification. METHODS: We sequenced TP53 in 30 SCCs from 6 women who had multiple SCCs on their legs during a 21-month time frame...
July 11, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28683740/using-kasp-technique-to-screen-lrrk2-g2019s-mutation-in-a-large-tunisian-cohort
#18
Zied Landoulsi, Sawssan Benromdhan, Mouna Ben Djebara, Mariem Damak, Hamza Dallali, Rym Kefi, Sonia Abdelhak, Amina Gargouri-Berrechid, Chokri Mhiri, Riadh Gouider
BACKGROUND: In North African populations, G2019S mutation in LRRK2 gene, encoding for the leucine-rich repeat kinase 2, is the most prevalent mutation linked to familial and sporadic Parkinson's disease (PD). Early detection of G2019S by fast genetic testing is very important to guide PD's diagnosis and support patients and their family caregivers for better management of their life according to disease's evolution. METHODS: In our study, a genetic PD's diagnosis tool was developed for large scale genotyping using Kompetitive Allele Specific PCR (KASP) technology...
July 6, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/28630621/development-of-snp-genotyping-assays-for-seed-composition-traits-in-soybean
#19
Gunvant Patil, Juhi Chaudhary, Tri D Vuong, Brian Jenkins, Dan Qiu, Suhas Kadam, Grover J Shannon, Henry T Nguyen
Seed composition is one of the most important determinants of the economic values in soybean. The quality and quantity of different seed components, such as oil, protein, and carbohydrates, are crucial ingredients in food, feed, and numerous industrial products. Soybean researchers have successfully developed and utilized a diverse set of molecular markers for seed trait improvement in soybean breeding programs. It is imperative to design and develop molecular assays that are accurate, robust, high-throughput, cost-effective, and available on a common genotyping platform...
2017: International Journal of Plant Genomics
https://www.readbyqxmd.com/read/28624908/development-and-validation-of-kasp-markers-for-the-greenbug-resistance-gene-gb7-and-the-hessian-fly-resistance-gene-h32-in-wheat
#20
Chor-Tee Tan, Hangjin Yu, Yan Yang, Xiangyang Xu, Mingshun Chen, Jackie C Rudd, Qingwu Xue, Amir M H Ibrahim, Lisa Garza, Shichen Wang, Mark E Sorrells, Shuyu Liu
Greenbug and Hessian fly are important pests that decrease wheat production worldwide. We developed and validated breeder-friendly KASP markers for marker-assisted breeding to increase selection efficiency. Greenbug (Schizaphis graminum Rondani) and Hessian fly [Mayetiola destructor (Say)] are two major destructive insect pests of wheat (Triticum aestivum L.) throughout wheat production regions in the USA and worldwide. Greenbug and Hessian fly infestation can significantly reduce grain yield and quality. Breeding for resistance to these two pests using marker-assisted selection (MAS) is the most economical strategy to minimize losses...
June 17, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
keyword
keyword
109821
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"