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https://www.readbyqxmd.com/read/28932231/addition-of-aegilops-u-and-m-chromosomes-affects-protein-and-dietary-fiber-content-of-wholemeal-wheat-flour
#1
Marianna Rakszegi, István Molnár, Alison Lovegrove, Éva Darkó, András Farkas, László Láng, Zoltán Bedő, Jaroslav Doležel, Márta Molnár-Láng, Peter Shewry
Cereal grain fiber is an important health-promoting component in the human diet. One option to improve dietary fiber content and composition in wheat is to introduce genes from its wild relatives Aegilops biuncialis and Aegilops geniculata. This study showed that the addition of chromosomes 2U(g), 4U(g), 5U(g), 7U(g), 2M(g), 5M(g), and 7M(g) of Ae. geniculata and 3U(b), 2M(b), 3M(b), and 7M(b) of Ae. biuncialis into bread wheat increased the seed protein content. Chromosomes 1U(g) and 1M(g) increased the proportion of polymeric glutenin proteins, while the addition of chromosomes 1U(b) and 6U(b) led to its decrease...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28922760/the-roles-of-pleiotropy-and-close-linkage-as-revealed-by-association-mapping-of-yield-and-correlated-traits-of-wheat-triticum-aestivum-l
#2
Albert W Schulthess, Jochen C Reif, Jie Ling, Jörg Plieske, Sonja Kollers, Erhard Ebmeyer, Viktor Korzun, Odile Argillier, Gunther Stiewe, Martin W Ganal, Marion S Röder, Yong Jiang
Grain yield (GY) of bread wheat (Triticum aestivum L.) is quantitatively inherited. Correlated GY-syndrome traits such as plant height (PH), heading date (HD), thousand grain weight (TGW), test weight (TW), grains per ear (GPE), and ear weight (EW) influence GY. Most quantitative genetics studies assessed the multiple-trait (MT) complex of GY-syndrome using single-trait approaches, and little is known about its underlying pleiotropic architecture. We investigated the pleiotropic architecture of wheat GY-syndrome through MT association mapping (MT-GWAS) using 372 varieties phenotyped in up to eight environments and genotyped with 18 832 single nucleotide polymorphisms plus 24 polymorphic functional markers...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28913806/endogenous-reference-genes-and-their-quantitative-real-time-pcr-assays-for-genetically-modified-bread-wheat-triticum-aestivum-l-detection
#3
Litao Yang, Sheng Quan, Dabing Zhang
Endogenous reference genes (ERG) and their derivate analytical methods are standard requirements for analysis of genetically modified organisms (GMOs). Development and validation of suitable ERGs is the primary step for establishing assays that monitoring the genetically modified (GM) contents in food/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ACC1, PKABA1, ALMT1, and Waxy-D1, as well as their performances in GM wheat analysis. Also, we discussed one model for developing and validating one ideal RG for one plant species based on our previous research work...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28913801/targeted-mutagenesis-in-hexaploid-bread-wheat-using-the-talen-and-crispr-cas-systems
#4
Yanpeng Wang, Yuan Zong, Caixia Gao
The use of sequence-specific transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats-associated system (CRISPR/Cas9) have provided powerful reverse genetic approaches to the targeted modification of genomes in numerous organisms. Both systems have been employed to generate loss-of-function alleles in bread wheat, by targeting multiple and single copies of genes. Here we present protocols for modifying the wheat genome using the two systems. The protocols include the design of TALEN and CRISPR/Cas9 target sites and their construction, evaluation of their activities in protoplasts, transformation of plants, and mutation screening...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28913798/agrobacterium-mediated-transformation-of-wheat-using-immature-embryos
#5
Goetz Hensel, Cornelia Marthe, Jochen Kumlehn
Methods of the Agrobacterium-mediated transformation of bread wheat (Triticum aestivum L.) have been improved in recent years so that genetic engineering can be routinely used for functional genomics as well as for wheat breeding. In the protocol described here, immature embryos of the spring-type model genotype Bobwhite SH 98 26 have been used. Preculture and temperature pretreatment of these explants have led to the reproducible generation of transgenic plants at efficiencies between 5 and 15%. Whereas primary transgenic plants regenerated in vitro commonly show reduced fitness and fertility, no apparent variations with regard to morphology and grain set in their transgenic progeny as compared to wild-type counterparts were observed...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28913791/enabling-molecular-technologies-for-trait-improvement-in-wheat
#6
Prem L Bhalla, Akanksha Sharma, Mohan B Singh
Wheat is the major staple food crop and a source of calories for humans worldwide. A steady increase in the wheat production is essential to meet the demands of an ever-increasing global population and to achieve food security. The large size and structurally intricate genome of polyploid wheat had hindered the genomic analysis. However, with the advent of new genomic technologies such as next generation sequencing has led to genome drafts for bread wheat and its progenitors and has paved the way to design new strategies for crop improvement...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28912827/development-of-taqman-probes-targeting-the-four-major-celiac-disease-epitopes-found-in-%C3%AE-gliadin-sequences-of-spelt-triticum-aestivum-ssp-spelta-and-bread-wheat-triticum-aestivum-ssp-aestivum
#7
Benjamin Dubois, Pierre Bertin, Yordan Muhovski, Emmanuelle Escarnot, Dominique Mingeot
BACKGROUND: Celiac disease (CD) is caused by specific sequences of gluten proteins found in cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta). Among them, the α-gliadins display the highest immunogenicity, with four T-cell stimulatory epitopes. The toxicity of each epitope sequence can be reduced or even suppressed according to the allelic form of each sequence. One way to address the CD problem would be to make use of this allelic variability in breeding programs to develop safe varieties, but tools to track the presence of toxic epitopes are required...
2017: Plant Methods
https://www.readbyqxmd.com/read/28912797/corrigendum-expression-level-of-the-dreb2-type-gene-identified-with-amplifluor-snp-markers-correlates-with-performance-and-tolerance-to-dehydration-in-bread-wheat-cultivars-from-northern-kazakhstan
#8
Yuri Shavrukov, Aibek Zhumalin, Dauren Serikbay, Makpal Botayeva, Ainur Otemisova, Aiman Absattarova, Grigoriy Sereda, Sergey Sereda, Vladimir Shvidchenko, Arysgul Turbekova, Satyvaldy Jatayev, Sergiy Lopato, Kathleen Soole, Peter Langridge
[This corrects the article on p. 1736 in vol. 7, PMID: 27917186.].
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28904802/analytical-study-of-226-ra-activity-concentration-in-market-consuming-foodstuffs-of-ramsar-iran
#9
M Gooniband Shooshtari, M R Deevband, M R Kardan, N Fathabadi, A A Salehi, K Naddafi, M Yunesian, R Nabizadeh Nodehi, M Karimi, S S Hosseini
BACKGROUND: Ramsar, a city of Iran located on the coast of the Caspian Sea, has been considered to be enormously important due to its high natural radioactivity levels. People living in High Level Natural Radiation Areas (HLNRAs) have been exposed by several sources, one of which could be foodstuff. However, many studies have been carried out to measure the environmental radioactivity in Ramsar, but no survey has been conducted in all stapled consumed foods yet. This study was dedicated to determine (226)Ra activity concentration in the daily diets of Ramsar residents as a probable exposure...
2017: Journal of Environmental Health Science & Engineering
https://www.readbyqxmd.com/read/28895793/food-stabilizing-antioxidants-increase-nutrient-bioavailability-in-the-in-vitro-model
#10
Magdalena Mika, Agnieszka Wikiera, Anna Antończyk, Maja Grabacka
OBJECTIVE: We investigated whether antioxidants may enhance bioavailability of lipids and carbohydrates and therefore increase the risk of obesity development. METHODS: We tested how supplementation with antioxidants (0.01% butylated hydroxytoluene [BHT], α-tocopherol, and green tea catechins) of a diet containing butter and wheat bread affects bioavailability of fats and carbohydrates. The absorption of the in vitro digested diet was estimated in the intestinal epithelia model of the Caco-2 cells cultured in Transwell chambers...
September 12, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28887628/development-of-a-set-of-pcr-markers-specific-to-aegilops-longissima-chromosome-arms-and-application-in-breeding-a-translocation-line
#11
Kunyang Wang, Zhishan Lin, Long Wang, Ke Wang, Qinghua Shi, Lipu Du, Xingguo Ye
Transcriptome data were used to develop 134 Aegilops longissima specific PCR markers and their comparative maps were constructed by contrasting with the homologous genes in the wheat B genome. Three wheat- Ae. longissima 1BL·1S (l) S translocation lines were identified using the correspondence markers. Aegilops longissima is an important wild species of common wheat that harbors many genes that can be used to improve various traits of common wheat (Triticum aestivum L.). To efficiently transfer the traits conferred by these Ae...
September 8, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28878789/effect-of-co-segregating-markers-on-high-density-genetic-maps-and-prediction-of-map-expansion-using-machine-learning-algorithms
#12
Amidou N'Diaye, Jemanesh K Haile, D Brian Fowler, Karim Ammar, Curtis J Pozniak
Advances in sequencing and genotyping methods have enable cost-effective production of high throughput single nucleotide polymorphism (SNP) markers, making them the choice for linkage mapping. As a result, many laboratories have developed high-throughput SNP assays and built high-density genetic maps. However, the number of markers may, by orders of magnitude, exceed the resolution of recombination for a given population size so that only a minority of markers can accurately be ordered. Another issue attached to the so-called 'large p, small n' problem is that high-density genetic maps inevitably result in many markers clustering at the same position (co-segregating markers)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28878329/rna-sequencing-and-co-expressed-long-non-coding-rna-in-modern-and-wild-wheats
#13
Halise Busra Cagirici, Burcu Alptekin, Hikmet Budak
There is an urgent need for the improvement of drought-tolerant bread and durum wheat. The huge and complex genome of bread wheat (BBAADD genome) stands as a vital obstruction for understanding the molecular mechanism underlying drought tolerance. However, tetraploid wheat (Triticum turgidum ssp., BBAA genome) is an ancestor of modern bread wheat and offers an important model for studying the drought response due to its less complex genome. Additionally, several wild relatives of tetraploid wheat have already shown a significant drought tolerance...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28877331/lactic-acid-bacteria-combinations-for-wheat-sourdough-preparation-and-their-influence-on-wheat-bread-quality-and-acrylamide-formation
#14
Elena Bartkiene, Vadims Bartkevics, Vita Krungleviciute, Iveta Pugajeva, Daiva Zadeike, Grazina Juodeikiene
Different lactic acid bacteria (LAB) from spontaneous wheat sourdough were isolated, identified, and characterized by their growth, acidification rate, and carbohydrate metabolism. The combinations of isolated LAB (Pediococcus pentosaceus LUHS183 and Leuconostoc mesenteroides LUHS242, P. pentosaceus LUHS183 and Lactobacillus brevis LUHS173, P. pentosaceus LUHS183 and Enterococcus pseudoavium LUHS 234, P. pentosaceus LUHS183 and Lactobacillus curvatus LUHS51, Lactobacillus plantarum LUHS135 and L. curvatus LUHS51, L...
September 6, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28875866/in-haitian-women-and-preschool-children-iron-absorption-from-wheat-flour-based-meals-fortified-with-sodium-iron-edta-is-higher-than-that-from-meals-fortified-with-ferrous-fumarate-and-is-not-affected-by-helicobacter-pylori-infection-in-children
#15
RANDOMIZED CONTROLLED TRIAL
Isabelle Herter-Aeberli, Kerline Eliancy, Yanick Rathon, Cornelia U Loechl, Joseline Marhône Pierre, Michael B Zimmermann
Fe fortification of wheat flour was proposed in Haiti to combat Fe deficiency, but Fe bioavailability from fortificants has never been investigated in Haitian women or preschool children, two key target groups. We aimed to investigate the bioavailability of ferrous fumarate (FeFum), NaFeEDTA and their combination from fortified wheat flour. We recruited twenty-two healthy mother-child pairs in Port au Prince, Haiti, for an Fe-absorption study. We administered stable Fe isotopes as FeFum or NaFeEDTA individually in low-extraction wheat flour bread rolls consumed by all participants in a randomised, cross-over design...
August 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28869377/saccharomyces-cerevisiae%C3%A2-and%C3%A2-kluyveromyces-marxianus%C3%A2-co-cultures-allow-to-reduce-fodmap-levels-in-whole-wheat-bread
#16
Nore Struyf, Jitka Laurent, Joran Verspreet, Kevin J Verstrepen, Christophe M Courtin
FODMAPs (Fermentable Oligo-, Di-, and Monosaccharides and Polyols) are small molecules that are poorly absorbed in the small intestine and rapidly fermented in the large intestine. There is evidence that a diet low in FODMAPs reduces abdominal symptoms in approx. 70% of the patients suffering from irritable bowel syndrome. Wheat contains relatively high fructan levels and is therefore a major source of FODMAPs in our diet. In this study, a yeast-based strategy was developed to reduce FODMAP levels in bread...
September 4, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28868214/allelic-variation-at-high-molecular-weight-and-low-molecular-weight-glutenin-subunit-genes-in-moroccan-bread-wheat-and-durum-wheat-cultivars
#17
Fatima Henkrar, Jamal El-Haddoury, Driss Iraqi, Najib Bendaou, Sripada M Udupa
Glutenin is a major protein fraction contributing to the functional properties of gluten and dough. The glutenin constitutes 30-40% of the protein in wheat flour and about half of that in gluten. It is essential to identify correct glutenin alleles and to improve wheat quality by selecting alleles that exert favorable effects. Moroccan wheat cultivars are unique in West Asia and North Africa region, since many of them possess resistance to Hessian fly, a pest, which is becoming important in other countries in the region...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28859373/phenotyping-of-field-grown-wheat-in-the-uk-highlights-contribution-of-light-response-of-photosynthesis-and-flag-leaf-longevity-to-grain-yield
#18
Elizabete Carmo-Silva, P John Andralojc, Joanna C Scales, Steven M Driever, Andrew Mead, Tracy Lawson, Christine A Raines, Martin A J Parry
Improving photosynthesis is a major target for increasing crop yields and ensuring food security. Phenotyping of photosynthesis in the field is critical to understand the limits to crop performance in agricultural settings. Yet, detailed phenotyping of photosynthetic traits is relatively scarce in field-grown wheat, with previous studies focusing on narrow germplasm selections. Flag leaf photosynthetic traits, crop development, and yield traits were compared in 64 field-grown wheat cultivars in the UK. Pre-anthesis and post-anthesis photosynthetic traits correlated significantly and positively with grain yield and harvest index (HI)...
June 15, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28857322/new-insights-into-structural-organization-and-gene-duplication-in-a-1-75-mb-genomic-region-harboring-the-%C3%AE-gliadin-gene-family-in-aegilops-tauschii-the-source-of-wheat-d-genome
#19
Naxin Huo, Lingli Dong, Shengli Zhang, Yi Wang, Tingting Zhu, Toni Mohr, Susan Altenbach, Zhiyong Liu, Jan Dvorak, Olin D Anderson, Ming-Cheng Luo, Daowen Wang, Yong Q Gu
Among the wheat prolamins important for its end-use traits, α-gliadins are the most abundant and also a major cause of food-related allergies and intolerances. Previous studies of various wheat species estimated between 25 to 150 α-gliadin genes reside in the Gli-2 locus regions. To better understand the evolution of this complex gene family, the DNA sequence of a 1.75-Mb genomic region spanning the Gli-2 locus was analyzed in the diploid grass, Aegilops tauschii, the ancestral source of D genome in hexaploid bread wheat...
August 30, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28846721/heritable-heading-time-variation-in-wheat-lines-with-the-same-number-of-ppd-b1-gene-copies
#20
Zuzana Ivaničová, Miroslav Valárik, Kateřina Pánková, Martina Trávníčková, Jaroslav Doležel, Jan Šafář, Zbyněk Milec
The ability of plants to identify an optimal flowering time is critical for ensuring the production of viable seeds. The main environmental factors that influence the flowering time include the ambient temperature and day length. In wheat, the ability to assess the day length is controlled by photoperiod (Ppd) genes. Due to its allohexaploid nature, bread wheat carries the following three Ppd-1 genes: Ppd-A1, Ppd-B1 and Ppd-D1. While photoperiod (in)sensitivity controlled by Ppd-A1 and Ppd-D1 is mainly determined by sequence changes in the promoter region, the impact of the Ppd-B1 alleles on the heading time has been linked to changes in the copy numbers (and possibly their methylation status) and sequence changes in the promoter region...
2017: PloS One
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