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wheat breading

Charoonsri Chusak, Christiani Jeyakumar Henry, Praew Chantarasinlapin, Varanya Techasukthavorn, Sirichai Adisakwattana
This study aimed to assess the effect of the Clitoria ternatea L. flower extract (CTE), on the inhibition of pancreatic α-amylase, in vitro starch hydrolysis, and predicted the glycemic index of different type of flours including potato, cassava, rice, corn, wheat, and glutinous rice flour. The application in a bakery product prepared from flour and CTE was also determined. The results demonstrated that the 1% and 2% (w/v) CTE inhibited the pancreatic α-amylase activity by using all flours as a substrate...
July 2, 2018: Foods (Basel, Switzerland)
Amanda Tafuri Paniago Passarinho, Rafaela Zandonade Ventorim, Gabriela Piccolo Maitan-Alfenas, Eduardo Basílio de Oliveira, Valéria Monteze Guimarães
BACKGROUND: Endo-1,4-β-xylanases have remarkable hydrolytic activity towards arabinoxylans and xylanase (xynA), produced by the anaerobic fungus Orpinomyces sp. strain PC-2 was shown to be superior to specific activity, which strongly suggests their applicability in the bakery industry, for the processing of whole-wheat flour containing xylans. In this way, two xylanases from this source, the Small Wild-Type xylanase (SWT) and the Small Mutant xylanase (SM2) (V108A, A199T), were expressed in E...
July 12, 2018: Journal of the Science of Food and Agriculture
Cecilia Lourdudoss, Laurent Arnaud, Alicja Wolk, Ronald F van Vollenhoven, Daniela Di Giuseppe
Objective: To investigate long-term dietary changes after rheumatoid arthritis (RA) diagnosis in Swedish women, compared to women without RA. Methods: This study included 21,602 women from the Swedish Mammography Cohort (SMC), who completed dietary questionnaires in 1997 and 2009. Between 1997 and 2009, 191 women were diagnosed with RA. Dietary changes after RA diagnosis were analyzed based on intake of 82 food items. Statistical analysis included linear mixed models...
2018: International Journal of Rheumatology
Teresa Fuertes-Mendizábal, José M Estavillo, Miren K Duñabeitia, Ximena Huérfano, Ander Castellón, Carmen González-Murua, Ana Aizpurua, María Begoña González-Moro
This work explores whether the natural abundance of N isotopes technique could be used to understand the movement of N within the plant during vegetative and grain filling phases in wheat crop ( Triticum aestivum L.) under different fertilizer management strategies. We focus on the effect of splitting the same N dose through a third late amendment at flag leaf stage (GS37) under humid Mediterranean conditions, where high spring precipitations can guarantee the incorporation of the lately applied N to the soil-plant system in an efficient way...
2018: Frontiers in Plant Science
Paul McManus, Joshua Hortin, Anne J Anderson, Astrid R Jacobson, David W Britt, Joseph Stewart, Joan E McLean
The impact of copper oxide nanoparticles (CuO NPs) on crop production is dependent on the biogeochemistry of Cu in the rooting zone of the plant. This study addressed the metabolites in wheat root exudates that increased dissolution of CuO NPs, and whether solubility correlated with Cu uptake into the plant. Bread wheat (Triticum aestivum cv. Dolores) was grown for 10 d with 0 to 300 mg Cu/kg as CuO NPs in sand, a matrix deficient in Fe, Zn, Mn and Cu for optimum plant growth. Increased NP doses enhanced root exudation, including the Cu-complexing phytosiderophore, 2'-deoxymugineic acid (DMA), and corresponded to greater dissolution of the CuO NPs...
July 5, 2018: Environmental Toxicology and Chemistry
Kyoungwon Cho, Hye-Rang Beom, You-Ran Jang, Susan B Altenbach, William H Vensel, Annamaria Simon-Buss, Sun-Hyung Lim, Min G Kim, Jong-Yeol Lee
Wheat gliadins are a complex group of proteins that contribute to the functional properties of wheat flour doughs and contain epitopes that are relevant for celiac disease (CD) and wheat-dependent exercise-induced anaphylaxis (WDEIA). In this study, we extracted ethanol-soluble gliadin fractions from flour of the Korean bread wheat cultivar Keumkang. Proteins were separated by 2-dimensional gel electrophoresis (2-DE) using a pI range of 6-11 in the first dimension and subjected to tandem mass spectrometry. α-, γ-, and ω-gliadins were identified as the predominant proteins in 31, 28, and one 2-DE spot, respectively...
2018: Frontiers in Plant Science
Adam Norman, Julian Taylor, James Edwards, Haydn Kuchel
Genomic selection applied to plant breeding enables earlier estimates of a line's performance and significant reductions in generation interval. Several factors affecting prediction accuracy should be well understood if breeders are to harness genomic selection to its full potential. We used a panel of 10,375 bread wheat ( Triticum aestivum ) lines genotyped with 18,101 SNP markers to investigate the effect and interaction of training set size, population structure and marker density on genomic prediction accuracy...
July 3, 2018: G3: Genes—Genomes—Genetics
Manjit Singh, Manish Kumar, Marc C Albertsen, Joshua K Young, A Mark Cigan
Hexaploid bread wheat is not readily amenable to traditional mutagenesis approaches. In this study, we show efficient utilization of CRISPR-Cas system and Next Generation Sequencing for mutant analysis in wheat. Identification and manipulation of male fertility genes in hexaploid bread wheat is important for understanding the molecular basis of pollen development and to obtain novel sources of nuclear genetic male sterility (NGMS). The maize Male sterile 45 (Ms45) gene encodes a strictosidine synthase-like enzyme and has been shown to be required for male fertility...
June 29, 2018: Plant Molecular Biology
Mirza Faisal Qaseem, Rahmatullah Qureshi, Quddoos H Muqaddasi, Humaira Shaheen, Rehana Kousar, Marion S Röder
Most investigations to date aiming to identify the genetic basis of the stress response of wheat (Triticum aestivum L.) have focused on the response to single stress agents such as high temperature or drought, even though in the natural situation, these stresses tend often to occur together. Here, a panel of 108 spring type bread wheat cultivars was phenotyped for 15 yield and yield related traits for two years (2014/15 and 2015/16) under non-stressed conditions, under high temperature stress, under drought and under a combined high temperature and drought regime...
2018: PloS One
Ainur Ismagul, Nannan Yang, Elina Maltseva, Gulnur Iskakova, Inna Mazonka, Yuri Skiba, Huihui Bi, Serik Eliby, Satyvaldy Jatayev, Yuri Shavrukov, Nikolai Borisjuk, Peter Langridge
BACKGROUND: The relatively low efficiency of biolistic transformation and subsequent integration of multiple copies of the introduced gene/s significantly complicate the genetic modification of wheat (Triticum aestivum) and other plant species. One of the key factors contributing to the reproducibility of this method is the uniformity of the DNA/gold suspension, which is dependent on the coating procedure employed. It was also shown recently that the relative frequency of single copy transgene inserts could be increased through the use of nanogram quantities of the DNA during coating...
June 26, 2018: BMC Plant Biology
Harinder Vishwakarma, Alim Junaid, Jayanand Manjhi, Gyanendra Pratap Singh, Kishor Gaikwad, Jasdeep Chatrath Padaria
To generate a genetic resource of heat stress responsive genes/ESTs, suppression subtractive hybridization (SSH) library was constructed in a heat and drought stress tolerant Indian bread wheat cultivar C306. Ninety three days old plants during grain filling stage were subjected to heat stress at an elevated temperature of 37°C and 42°C for different time intervals (30 min, 1h, 2h, 4h, and 6h). Two subtractive cDNA libraries were prepared with RNA isolated from leaf samples at 37°C and 42°C heat stress...
2018: PloS One
Jussi Loponen, Michael G Gänzle
A low FODMAP (fermentable oligosaccharides, disaccharides, monosaccharides, and polyols) diet allows most irritable bowel syndrome (IBS) patients to manage their gastrointestinal symptoms by avoiding FODMAP-containing foods, such as onions, pulses, and products made from wheat or rye. The downside of a low FODMAP diet is the reduced intake of dietary fiber. Applying sourdoughs—with specific FODMAP-targeting metabolic properties—to wholegrain bread making can help to remarkably reduce the content of FODMAPs in bread without affecting the content of the slowly fermented and well-tolerated dietary fiber...
June 22, 2018: Foods (Basel, Switzerland)
Mehdi Mansouri, Mohammad Reza Naghavi, Hoshang Alizadeh, Ghasem Mohammadi-Nejad, Seyed Ahmad Mousavi, Ghasem Hosseini Salekdeh, Yuichi Tada
Aegilops tauschii is the diploid progenitor of the bread wheat D-genome. It originated from Iran and is a source of abiotic stress tolerance genes. However, little is known about the molecular events of salinity tolerance in Ae. tauschii. This study investigates the leaf transcriptional changes associated with long-term salt stress. Total RNA extracted from leaf tissues of control and salt-treated samples was sequenced using the Illumina technology, and more than 98 million high-quality reads were assembled into 255,446 unigenes with an average length of 1398 bp and an N50 of 2269 bp...
June 21, 2018: Functional & Integrative Genomics
Meifang Qi, Zijuan Li, Chunmei Liu, Wenyan Hu, Luhuan Ye, Yilin Xie, Yili Zhuang, Fei Zhao, Wan Teng, Qi Zheng, Zhenjun Fan, Lin Xu, Zhaobo Lang, Yiping Tong, Yijing Zhang
Genetic diversity in plants is remarkably high. Recent whole genome sequencing (WGS) of 67 rice accessions recovered 10,872 novel genes. Comparison of the genetic architecture among divergent populations or between crops and wild relatives is essential for obtaining functional components determining crucial traits. However, many major crops have gigabase-scale genomes, which are not well-suited to WGS. Existing cost-effective sequencing approaches including re-sequencing, exome-sequencing and restriction enzyme-based methods all have difficulty in obtaining long novel genomic sequences from highly divergent population with large genome size...
June 21, 2018: Nucleic Acids Research
Panfeng Guan, Lahu Lu, Lijia Jia, Muhammad Rezaul Kabir, Jinbo Zhang, Tianyu Lan, Yue Zhao, Mingming Xin, Zhaorong Hu, Yingyin Yao, Zhongfu Ni, Qixin Sun, Huiru Peng
Major advances in wheat production are needed to address global food insecurity under future climate conditions, such as high temperatures. The grain yield of bread wheat ( Triticum aestivum L.) is a quantitatively inherited complex trait that is strongly influenced by interacting genetic and environmental factors. Here, we conducted global QTL analysis for five yield-related traits, including spike yield, yield components and plant height (PH), in the Nongda3338/Jingdong6 doubled haploid (DH) population using a high-density SNP and SSR-based genetic map...
2018: Frontiers in Plant Science
Liangkui Li, Georg Lietz, Wendy Bal, Anthony Watson, Ben Morfey, Chris Seal
A number of epidemiological studies have suggested that diets rich in whole grains are linked to lower cardiovascular disease (CVD) risk and mortality. Quinoa, a pseudo-cereal, is included in the “whole grain” category but the effects of quinoa consumption in humans is not widely studied. Our aim was to undertake a dietary intervention study to investigate the effects of daily consumption of quinoa-enriched bread (providing 20 g quinoa flour) on CVD risk markers compared with a 100% refined wheat bread control...
June 16, 2018: Nutrients
Ana Luísa Garcia-Oliveira, César Benito, Henrique Guedes-Pinto, Paula Martins-Lopes
Recently, members of MATE family have been implicated in aluminium (Al) tolerance by facilitating citrate efflux in plants. The aim of the present work was to perform a molecular characterisation of the MATE2 gene in bread wheat. Here, we cloned a member of the MATE gene family in bread wheat and named TaMATE2, which showed the typical secondary structure of MATE-type transporters maintaining all the 12 transmembrane domains. The amplification in Chinese Spring nulli-tetrasomic and ditelosomic lines revealed that TaMATE2 is located on the long arm of homoeologous group 1 chromosomes...
June 15, 2018: Plant Biology
Honghong Wu, Lana Shabala, Elisa Azzarello, Yuqing Huang, Camilla Pandolfi, Nana Su, Qi Wu, Shengguan Cai, Nadia Bazihizina, Lu Wang, Meixue Zhou, Stefano Mancuso, Zhonghua Chen, Sergey Shabala
The progress in plant breeding for salinity stress tolerance is handicapped by the lack of understanding of the specificity of salt stress signalling and adaptation at the cellular and tissue levels. In this study, we used electrophysiological, fluorescence imaging, and real-time quantitative PCR tools to elucidate the essentiality of the cytosolic Na+ extrusion in functionally different root zones (elongation, meristem, and mature) in a large number of bread and durum wheat accessions. We show that the difference in the root's ability for vacuolar Na+ sequestration in the mature zone may explain differential salinity stress tolerance between salt-sensitive durum and salt-tolerant bread wheat species...
June 11, 2018: Journal of Experimental Botany
Olena Prykhodko, Jonna Sandberg, Stephen Burleigh, Inger Björck, Anne Nilsson, Frida Fåk Hållenius
Rye kernel bread (RKB) evening meals improve glucose tolerance, enhance appetite regulation and increase satiety in healthy volunteers. These beneficial effects on metabolic responses have been shown to be associated with increased gut fermentation. The present study aimed to elucidate if RKB evening meals may cause rapid alterations in microbiota composition that might be linked to metabolic-, immune-, and appetite- parameters. Gut-brain axis interaction was also studied by relating microbiota composition to amount of brain-derived neurotrophic factor (BDNF) in blood plasma...
2018: Frontiers in Nutrition
Han Tao, Ji-Sheng Huang, Qiu-Tao Xie, Yong-Ming Zou, Hui-Li Wang, Xue-Ying Wu, Xue-Ming Xu
Properties of starches isolated from soft and hard wheat dough after freezing/thawing (F/T) treatment were investigated. Significance of results was observed between isolated hard wheat starch (HWS) and soft wheat starch (SWS), but both cultivars showed an increase in the amounts of damaged starch and leaching proteins, lipids, and amylose with F/T cycles. The freezing-treated HWS exhibited a higher swelling power and peak, trough, breakdown and final viscosity than SWS after F/T treatment. The onset, peak and conclusion gelatinization temperatures and the enthalpy of the isolated HWS determined by differential scanning calorimetry, decreased throughout F/T cycles...
November 1, 2018: Food Chemistry
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