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Non-healing wounds treatments

N Kirsten, K Herberger, M Augustin, W Tigges, C Behrendt, F Heidemann, E S Debus, H Diener
According to the Research Group for Primary Medical Care (PMV), approximately 890,000 people in Germany were suffering from a chronic wound in 2012. This corresponds to a prevalence of 1.1%. Ulcus cruris, diabetic ulcers and decubital ulcers are among the most frequent causes of chronic wounds (57-80%). The guarantee for successful wound care is based on a good understanding of the physiology of the wound healing process. A disorder of the phase-like course can lead to complications, delays or suspension of wound healing...
September 21, 2018: Der Chirurg; Zeitschrift Für Alle Gebiete der Operativen Medizen
Deborah Chicharro, Jose M Carrillo, Mónica Rubio, Ramón Cugat, Belén Cuervo, Silvia Guil, Jerónimo Forteza, Victoria Moreno, Jose M Vilar, Joaquín Sopena
BACKGROUND: The use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine. In recent years, human and veterinary medicine prefer to avoid using traumatic techniques and choose low or non-invasive procedures. The objective of this study was to evaluate the efficacy of PRGF, ASCs and the combination of both in wound healing of full-thickness skin defects in rabbits. With this purpose, a total of 144 rabbits were used for this study...
September 21, 2018: BMC Veterinary Research
Bi O Jeong, Duke Whan Chung, Jong Hun Baek
RATIONALE: Calcific myonecrosis is a very rare late sequela that occurs in patients who have had trauma accompanied by vascular compromise, in which a single muscle or entire muscles in a compartment undergoes necrosis and form a calcified mass. It is mostly a benign entity, but some cases cause bone destruction and form non-healing chronic sinuses. In such cases, wound management becomes difficult and there is a potential risk of secondary infection. PATIENT CONCERNS: A 60-year-old male was referred for evaluation of a pain, erythematous changes, and draining sinus of the anterolateral aspect of his left leg...
September 2018: Medicine (Baltimore)
Kegang Jia, Fan Chen, Lian Xu
OBJECTIVES: This study is aimed to explore the functions of long noncoding RNA (lncRNA) HEIH on the proliferation as well as metastasis of non-small cell lung cancer (NSCLC). METHODS: HEIH was shown to be increased in NSCLC by a microarray study on lncRNA profiling. We detected the expression of HEIH in different human NSCLC lines and tissues by quantitative real-time PCR. After gain- and loss-of-function approaches in A549 and Calu-1 cells, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, thiazolyl blue tetrazolium bromide (MTT) assays and a colony formation assay were applied to test the proliferative activity of cells, and the apoptosis of cells was measured by flow cytometry...
September 19, 2018: Journal of Cellular Biochemistry
Marek Konop, Joanna Czuwara, Ewa Kłodzińska, Anna K Laskowska, Urszula Zielenkiewicz, Iwona Brzozowska, Seyed M Nabavi, Lidia Rudnicka
Impaired wound healing is a major medical problem in diabetes. The objective of this study was to determine the possible application of an insoluble fraction of fur-derived keratin biomaterial as a wound dressing in a full thickness surgical skin wound model in mice ( n = 20) with iatrogenically induced diabetes. The obtained keratin dressing was examined in vitro and in vivo. In vitro study showed the keratin dressing is tissue biocompatible and non-toxic for murine fibroblasts. Antimicrobial examination revealed the keratin dressing inhibited the growth of S...
September 18, 2018: Journal of Biomaterials Applications
Zealyn Shi Lin Heng, Jing Yi Lee, Charannya Sozheesvari Subhramanyam, Cheng Wang, Lal Zo Thanga, Qidong Hu
A majority of breast cancer cases are positive for the estrogen receptor (ER), which means that they can respond to the estrogen hormone to achieve growth. Hence, the ER signaling pathway has been extensively targeted in pharmaceutical research and development in order to suppress tumor growth. However, prevalent hormone therapy and targeted therapy often become ineffective as cancer cells ultimately develop resistance, suggesting that there could be unidentified signaling molecules and events that regulate breast cancer growth...
November 2018: Oncology Reports
S Borys, A H Ludwig-Slomczynska, M Seweryn, J Hohendorff, T Koblik, J Machlowska, B Kiec-Wilk, P Wolkow, Maciej T Malecki
AIMS: Negative pressure wound therapy (NPWT) has been successfully used as a treatment for diabetic foot ulceration (DFU). Its mechanism of action on the molecular level, however, is not fully understood. We assessed the effect of NPWT on gene expression in patients with type 2 diabetes (T2DM) and DFU. METHODS: We included two cohorts of patients-individuals treated with either NPWT or standard therapy. The assignment to NWPT was non-randomized and based on wound characteristics...
September 17, 2018: Acta Diabetologica
Lemeng Sun, Liangshu Feng, Jiuwei Cui
BACKGROUND: Hepatocellular carcinoma (HCC) is the second leading cause of cancer death in Asia; however, the molecular mechanism in its tumorigenesis remains unclear. Abnormal expression of claudins (CLDNs), a family of tight junction (TJ) proteins, plays an important role in the metastatic phenotype of epithelial-derived tumors by affecting tight junction structure, function and related cellular signaling pathways. In a previous study, we used a tissue chip assay to identify CLDN17 as an upregulated gene in HCC...
September 15, 2018: Diagnostic Pathology
Jun Zou, Yan Yang, Yang Yang, Xingren Liu
Non-small cell lung cancer (NSCLC) is the leading cause of cancer death. Particularly, inflammation is crucial for the progression of NSCLC. In the past few decades, the anti-tumor effects of some traditional Chinese medicinal herbs have caused much attention. This study was designed to investigate the effects of polydatin in the progression of NSCLC. CCK-8 assay, wound healing assay and western blot assay were used to assess the anti-cancer property. Consequently, we showed that polydatin inhibited proliferation and migration of NSCLC cells (A549 and H1299 cells) in a dose-dependent manner...
September 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Divya B Patel, Marco Santoro, Louis J Born, John P Fisher, Steven M Jay
Extracellular vesicles (EVs), including exosomes, microvesicles, and others, have emerged as potential therapeutics for a variety of applications. Pre-clinical reports of EV efficacy in treatment of non-healing wounds, myocardial infarction, osteoarthritis, traumatic brain injury, spinal cord injury, and many other injuries and diseases demonstrate the versatility of this nascent therapeutic modality. EVs have also been demonstrated to be effective in humans, and clinical trials are underway to further explore their potential...
September 12, 2018: Biotechnology Advances
Edimara S Reis, Nadja Berger, Xin Wang, Sophia Koutsogiannaki, Robert K Doot, Justin T Gumas, Periklis G Foukas, Ranillo R G Resuello, Joel V Tuplano, David Kukis, Alice F Tarantal, Anthony J Young, Tetsushiro Kajikawa, Athena Soulika, Dimitrios C Mastellos, Despina Yancopoulou, Ali-Reza Biglarnia, Markus Huber-Lang, George Hajishengallis, Bo Nilsson, John D Lambris
The central component of the complement cascade, C3, is involved in various biological functions, including opsonization of foreign bodies, clearance of waste material, activation of immune cells, and triggering of pathways controlling development. Given its broad role in immune responses, particularly in phagocytosis and the clearance of microbes, a deficiency in complement C3 in humans is often associated with multiple bacterial infections. Interestingly, an increased susceptibility to infections appears to occur mainly in the first two years of life and then wanes throughout adulthood...
September 11, 2018: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Ho-Ryun Won, Sung Un Kang, Haeng Jun Kim, Jeon Yeob Jang, Yoo Seob Shin, Chul-Ho Kim
Adequate and rapid mucosal regeneration is one of the most important factors in the healing process of nasal mucosa after surgery or trauma. In particular, delayed mucosal regeneration after surgery is an important cause of surgical failure. However, no effective treatment is available yet. Non-thermal plasma (NTP) has several medical effects, but the existing probe type is limited to local direct treatment. Therefore, we investigated the various effects using liquid type plasma to overcome this limitation...
September 13, 2018: Scientific Reports
Marieke Bamps, Rüveyda Dok, Sandra Nuyts
Objectives: Low-level laser therapy (LLLT) is a promising non-invasive treatment option for oropharyngeal mucositis, which is a common side effect of many oncological treatments. LLLT is known for its wound healing properties due to the stimulation of cellular processes, such as proliferation, migration and differentiation. Controversy exists on the possible stimulatory effect of LLLT on head and neck cancer (HNSCC) cells in patients treated with radiotherapy. The aim of this study was to evaluate the biostimulatory effect together with the underlying mechanisms of LLLT on HNSCC cancer cells and normal epithelial cells...
2018: Frontiers in Oncology
Ana Livia Silva Galbiatti-Dias, Glaucia Maria Mendonça Fernandes, Marcia Maria Urbanin Castanhole-Nunes, Luiza Fernandes Hidalgo, Carlos Henrique Viesi Nascimento Filho, Rosa Sayoko Kawasaki-Oyama, Leticia Antunes Muniz Ferreira, Patricia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo
Recent evidence suggests that cancer stem cells (CSCs), a small population of cancer cells that are highly tumourigenic, capable of self-renewal and have the ability to differentiate into cells that constitute the tumor, are the "drivers" of local recurrence and metastatic spread and may be associated with resistant to conventional therapy. The objectives of the study are to identify and characterize two head and neck cancer cell lines with regard CD44high /CD133high /CD117high profile (CSCs) and CD44low /CD133low /CD117low profile (Non-CSCs); to investigate the influence of chemotherapy treatment in CSCs and compare with Non-CSCs; to evaluate CD44 and EGFR gene expression in CSCs...
2018: American Journal of Cancer Research
Maryam Rameshk, Fariba Sharififar, Mitra Mehrabani, Abbas Pardakhty, Alireza Farsinejad, Mehrnaz Mehrabani
PURPOSE: In Traditional Persian Medicine (TPM), different natural treatments have been suggested for skin damages such as Narcissus tazetta L. bulb application. New drug delivery systems such as niosomes have shown considerable increase transdermal drug delivery through stratum corneum, the main barrier against substances transport into skin. The aim of this study is preparation of niosomal formulations from N. tazetta bulb extract and evaluation of its in vitro wound healing effect. MATERIALS AND METHODS: Non-ionic surfactant vesicles (NSVs or niosomes) were prepared by film hydration method from percolated extract of N...
September 12, 2018: Daru: Journal of Faculty of Pharmacy, Tehran University of Medical Sciences
Taylor R Johnson, Belinda I Gómez, Matthew K McIntyre, Michael A Dubick, Robert J Christy, Susannah E Nicholson, David M Burmeister
The ecological community of microorganisms in/on humans, termed the microbiome, is vital for sustaining homeostasis. While culture-independent techniques have revealed the role of the gut microbiome in human health and disease, the role of the cutaneous microbiome in wound healing is less defined. Skin commensals are essential in the maintenance of the epithelial barrier function, regulation of the host immune system, and protection from invading pathogenic microorganisms. In this review, we summarize the literature derived from pre-clinical and clinical studies on how changes in the microbiome of various acute and chronic skin wounds impact wound healing tissue regeneration...
September 11, 2018: International Journal of Molecular Sciences
Yashar Mehrbani Azar, Robyn Green, Carola U Niesler, Mari van de Vyver
Mesenchymal stem cells (MSCs) are a promising therapeutic tool for the treatment of non-healing diabetic wounds. The pathological nature of the niche microenvironment limits the use of autologous cell therapy in diabetic patients. Prolonged exposure of endogenous MSCs to a pathological micro-environment in vivo, reduces their ability to respond to environmental cues. This study investigated the effectiveness of ex vivo antioxidant treatment [N-acetylcysteine (7.5mM NAC) and Ascorbic acid 2-phosphate (0.6mM AAP)] to restore the paracrine function of diabetic MSCs...
September 6, 2018: Stem Cells and Development
Huanling Lai, Yuwei Wang, Fugang Duan, Ying Li, Zebo Jiang, Lianxiang Luo, Liang Liu, Elaine L H Leung, Xiaojun Yao
Oncogenic activation of the KRAS gene via point mutations occurs in 20-30% of patients with non-small cell lung cancer (NSCLC). The RAS-RAF-ERK and RAS-PI3K-AKT pathways are the major hyper-activated downstream pathways in RAS mutation, which promotes the unlimited lifecycle of cancer cells and their metastasis in humans. However, the success of targeted therapy is restricted by many factors. Herein, we show a new pharmacological KRAS signaling inhibitor krukovine, which is a small molecular bisbenzylisoquinoline alkaloid, isolated from the bark of Abuta grandifolia (Mart...
2018: Frontiers in Pharmacology
Masakazu Kurita, Toshikazu Araoka, Tomoaki Hishida, David D O'Keefe, Yuta Takahashi, Akihisa Sakamoto, Masahiro Sakurai, Keiichiro Suzuki, Jun Wu, Mako Yamamoto, Reyna Hernandez-Benitez, Alejandro Ocampo, Pradeep Reddy, Maxim Nikolaievich Shokhirev, Pierre Magistretti, Estrella Núñez Delicado, Hitomi Eto, Kiyonori Harii, Juan Carlos Izpisua Belmonte
Large cutaneous ulcers are, in severe cases, life threatening1,2 . As the global population ages, non-healing ulcers are becoming increasingly common1,2 . Treatment currently requires the transplantation of pre-existing epithelial components, such as skin grafts, or therapy using cultured cells2 . Here we develop alternative supplies of epidermal coverage for the treatment of these kinds of wounds. We generated expandable epithelial tissues using in vivo reprogramming of wound-resident mesenchymal cells. Transduction of four transcription factors that specify the skin-cell lineage enabled efficient and rapid de novo epithelialization from the surface of cutaneous ulcers in mice...
September 2018: Nature
F Riechelmann, P Kaiser, R Arora
OBJECTIVE: Debridement of soft tissue and bone in an open fracture situation to minimize infection risk and achieve primary skin closure, or to provide conditions for early soft tissue coverage. INDICATIONS: Indications are Gustilo-Anderson grade I-III A-C open fractures. CONTRAINDICATIONS: Contraindications are injuries requiring amputation, burns, and life-threatening injuries which make appropriate treatment temporarily impossible. SURGICAL TECHNIQUE: Removal of gross contamination and macroscopic contaminants; debridement of the wound; complete resection of contaminated and dirty tissue; sparse step-by-step resection of contaminated or non-vital wound and bone margins until vital, bleeding tissue begins; low-pressure irrigation with isotonic irrigation fluid; diagnostic biopsies for microbiological testing; reduction of dead space by interpositioning of muscle or cement spacers loaded with local antibiotics; primary wound closure if tension-free closure possible; otherwise, if resources and knowhow permit and satisfactory clean debridement was achieved, local flap; if flap impossible, debridement not satisfactory, secondary tissue necrosis likely, potential remaining contamination or contamination with fecal matter, then vacuum-assisted closure therapy...
September 4, 2018: Operative Orthopädie und Traumatologie
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