keyword
MENU ▼
Read by QxMD icon Read
search

antibiotics resistant genes

keyword
https://www.readbyqxmd.com/read/28437228/first-case-of-ndm-1-producing-klebsiella-pneumoniae-in-annaba-university-hospital-algeria
#1
Amel Abderrahim, Nassima Djahmi, Charlotte Pujol, Sabina Nedjai, Mohamed Cherif Bentakouk, Djamila Kirane-Gacemi, Mazouz Dekhil, Albert Sotto, Jean-Philippe Lavigne, Alix Pantel
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive...
February 22, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437226/first-report-of-prevalence-of-ctx-m-15-producing-escherichia-coli-o25b-st131-from-iran
#2
Mohammad Hasan Namaei, Masoud Yousefi, Masoud Ziaee, Alireza Salehabadi, Malaknaz Ghannadkafi, Elham Amini, Parvin Askari
The emergence of Escherichia coli sequence type 131 (ST131) as a multidrug-resistant and virulent pathogen represents a major challenge to public health globally. Recently, the O25b/ST131 E. coli producing CTX-M-15 with high virulence potential has been reported worldwide, but has received little attention in Iran. This study is the first in Iran to specifically determine the spread of the O25b/ST131 clone producing CTX-M-15 among E. coli isolates belonging to the B2 phylogenetic group. ST131 clone in phylogenetic group B2 was detected based on PCR detection of ST131-specific single-nucleotide polymorphisms in mdh and gyrB...
February 16, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28435890/gene-expression-profile-of-human-cytokines-in-response-to-burkholderia-pseudomallei-infection
#3
Shivankari Krishnananthasivam, Harindra Darshana Sathkumara, Enoka Corea, Mohan Natesan, Aruna Dharshan De Silva
Melioidosis is an underreported infectious disease, caused by the Gram-negative bacterium Burkholderia pseudomallei. Understanding the disease susceptibility and pathogenesis is crucial for developing newer diagnostic and therapeutic strategies for this life-threatening infection. In this study, we aimed to analyze the gene expression levels of important cytokines in melioidosis patients and establish useful correlates with disease biomarkers compared to cases of sepsis infection caused by other pathogens and healthy individuals...
March 2017: MSphere
https://www.readbyqxmd.com/read/28435889/multidrug-resistance-salmonella-genomic-island-1-in-a-morganella-morganii-subsp-morganii-human-clinical-isolate-from-france
#4
Eliette Schultz, Olivier Barraud, Jean-Yves Madec, Marisa Haenni, Axel Cloeckaert, Marie-Cécile Ploy, Benoît Doublet
Salmonella genomic island 1 (SGI1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous Salmonella enterica serovars and also in Proteus mirabilis. A serious threat to public health was revealed in the recent description in P. mirabilis of a SGI1-derivative multidrug resistance island named PGI1 (Proteus genomic island 1) carrying extended-spectrum-β-lactamase (ESBL) and metallo-β-lactamase resistance genes, blaVEB-6 and blaNDM-1, respectively...
March 2017: MSphere
https://www.readbyqxmd.com/read/28435202/phenotypic-and-genotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-from-bovine-mastitis
#5
S Hamid, Mohd Altaf Bhat, Irfan Ahmad Mir, Anil Taku, Gulzar Ahmad Badroo, Salik Nazki, Andleeb Malik
AIM: This study was conducted to determine the occurrence of methicillin-sensitive and Staphylococcus aureus (MSSA) methicillin-resistant S. aureus (MRSA) from bovine mastitis and to characterize them with respect to antibiotic resistance gene mecA. MATERIALS AND METHODS: A total of 160 mastitic milk samples were screened for the presence of S. aureus. The presumptive positive isolates were confirmed using nuc and 23S rRNA gene-based polymerase chain reaction. All the confirmed isolates were subjected to in vitro antibiogram using a number of antibiotics...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28435108/molecular-characterization-and-antimicrobial-susceptibility-of-the-ca-mrsa-isolated-from-healthcare-workers-tehran-iran
#6
Solmaz Ohadian Moghadam, Mohammad Modoodi Yaghooti, Nasrin Pourramezan, Mohammad Reza Pourmand
INTRODUCTION: Methicillin resistant Staphylococcus aureus (MRSA) has become as a nosocomial pathogen worldwide. Considering the importance of MRSA typing for understanding the evolution and dissemination of these strains, we studied the molecular characteristics of MRSA colonized healthcare workers (HCWs). METHODOLOGY: All MRSA isolated from HCWs, were genotyped using staphylococcal cassette chromosome mec (SCCmec) with multiplex PCR assay, multilocus sequence typing (MLST) and spa typing...
April 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28434779/a-comparative-study-of-cutibacterium-propionibacterium-acnes-clones-from-acne-patients-and-healthy-controls
#7
H B Lomholt, C F P Scholz, H Brüggemann, H Tettelin, M Kilian
BACKGROUND: Cutibacterium (Propionibacterium) acnes is assumed to play an important role in the pathogenesis of acne. OBJECTIVES: To examine if clones with distinct virulence properties are associated with acne. METHODS: Multiple C. acnes isolates from follicles and surface skin of patients with moderate to severe acne and healthy controls were characterized by multilocus sequence typing. To determine if CC18 isolates from acne patients differ from those of controls in the possession of virulence genes or lack of genes conducive to a harmonious coexistence the full genomes of dominating CC18 follicular clones from six patients and five controls were sequenced...
April 18, 2017: Anaerobe
https://www.readbyqxmd.com/read/28433796/genetic-diversity-and-virulence-genes-of-salmonella-enterica-subspecies-enterica-serotype-enteritidis-isolated-from-meats-and-eggs
#8
Fatemeh Fardsanei, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Taghi Zahraei Salehi, Mahmood Mahmoodi, Hamed Memariani, Farhad Nikkhahi
Salmonella enterica subspecies enterica serotype Enteritidis (S. Enteritidis) is one of the leading causes of food-borne gastroenteritis associated with the consumption of contaminated food products of animal origin. Little is known about the genetic diversity and virulence content of S. Enteritidis isolated from poultry meats and eggs in Iran. A total of 34 S. Enteritidis strains were collected from different food sources of animal origin in Tehran from May 2015 to July 2016. All of the S. Enteritidis strains were serotyped, antimicrobial susceptibility tested, and characterized for virulence genes...
April 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28432950/effects-of-chlortetracycline-and-copper-on-tetracyclines-and-copper-resistance-genes-and-microbial-community-during-swine-manure-anaerobic-digestion
#9
Rui Wang, Meixue Chen, Feng Feng, Junya Zhang, Qianwen Sui, Juan Tong, Yuansong Wei, Dongbin Wei
As antibiotic and heavy metals are over used in the livestock industry, animal manure is a reservoir of antibiotic resistance genes (ARGs). Anaerobic digestion has been reported to have the potential to reduce ARGs. However, few studies investigated whether reduction of ARGs would be affected by different external pressures including antibiotics and heavy metals during anaerobic digestion. The purpose of this study was thus to investigate effects of both chlortetracycline (CTC) and Cu on reduction of ARGs, heavy metal resistance genes (HMRGs) and mobile genetic elements (MGEs) during the swine manure anaerobic digestion...
March 25, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28430673/antimicrobial-resistant-streptococcus-pneumoniae-prevalence-mechanisms-and-clinical-implications
#10
Regine Cherazard, Marcia Epstein, Thien-Ly Doan, Tanzila Salim, Sheena Bharti, Miriam A Smith
BACKGROUND: Streptococcus pneumoniae is a major cause of pneumonia, meningitis, sepsis, bacteremia, and otitis media. S. pneumoniae has developed increased resistance to multiple classes of antibiotics. STUDY DESIGN: Systematic literature review of prevalence, mechanisms, and clinical implications in S. pneumoniae resistance. AREAS OF UNCERTAINTY: Since S. pneumoniae resistance to penicillin was first reported with subsequent development of resistance to other classes of drugs, selection of appropriate antibiotic treatment is challenging...
April 19, 2017: American Journal of Therapeutics
https://www.readbyqxmd.com/read/28429335/imipenem-resistance-in-serratia-marcescens-is-mediated-by-plasmid-expression-of-kpc-2
#11
W-Q Su, Y-Q Zhu, N-M Deng, L Li
OBJECTIVE: Imipenem is a broad-spectrum carbapenem antibiotic with applications against severe bacterial infections. Here, we describe the identification of imipenem-resistant Serratia marcescens in our hospital and the role of plasmid-mediated KPC-2 expression in imipenem resistance. MATERIALS AND METHODS: We used the modified Hodge test to detect carbapenemase produced in imipenem-resistant strains. RESULTS: His resistance can be transferred to E...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28427842/predominance-of-international-clone-2-oxa-23-producing-acinetobacter-baumannii-clinical-isolates-in-greece-2015-results-of-a-nationwide-study
#12
Spyros Pournaras, Konstantina Dafopoulou, Mariateresa Del Franco, Olympia Zarkotou, Evangelia Dimitroulia, Efi Protonotariou, Aggeliki Poulou, Raffaele Zarrilli, Athanasios Tsakris
In a previous nationwide study in Greece, OXA-58 was the sole carbapenemase present among carbapenem-resistant Acinetobacter baumannii (CRAB) isolated between 2000 and 2009. In this study, the antibiotic resistances, carbapenemase gene content and clonal relatedness of 194 single-patient CRAB clinical isolates collected randomly during 2015 from 11 tertiary hospitals located throughout Greece were investigated. Antimicrobial susceptibility was determined using commercial and dilution methods. PCR for carbapenemase genes were performed...
April 18, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28426273/preclinical-development-of-bcg-hiva-2auxo-int-harboring-an-integrative-expression-vector-for-a-hiv-tb-pediatric-vaccine-enhancement-of-stability-and-specific-hiv-1-t-cell-immunity
#13
Aakash Mahant, Narcís Saubi, Yoshiki Eto, Núria Guitart, Josep M ª Gatell, Tomáš Hanke, Joan Joseph
One of the critical issues that should be addressed in the development of a BCG-based HIV vaccine is genetic plasmid stability. Therefore, to address this issue we have considered using integrative vectors and the auxotrophic mutant of BCG complemented with a plasmid carrying a wild-type complementing gene. In this study, we have constructed an integrative E. coli-mycobacterial shuttle plasmid, p2auxo.HIVA(int), expressing the HIV-1 clade A immunogen HIVA. This shuttle vector employs an antibiotic resistance-free mechanism for plasmid selection and maintenance...
April 20, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28426231/the-prevalence-of-integrons-as-the-carrier-of-antibiotic-resistance-genes-in-natural-and-man-made-environments
#14
Liping Ma, An-Dong Li, Xiao-Le Yin, Tong Zhang
Class 1 integrase intI1 has been considered as a good proxy for anthropogenic pollution because of being linked to genes conferring resistance to antibiotics. The gene cassettes of class 1 integrons could carry diverse antibiotic resistance genes (ARGs) and conduct horizontal gene transfer among microorganisms. The present study applied high-throughput sequencing technique combined with an intI1 database and genome assembly to quantify the abundance of intI1 in 64 environmental samples from 8 ecosystems, and to investigate the diverse arrangements of ARG-carrying gene cassettes (ACGCs) carried by class 1 integrons...
April 20, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28425935/water-access-sanitation-and-hygiene-conditions-and-health-outcomes-among-two-settlement-types-in-rural-far-north-cameroon
#15
Tyler J Gorham, Joshua Yoo, Rebecca Garabed, Arabi Mouhaman, Jiyoung Lee
The Far North region in Cameroon has been more heavily impacted by cholera than any other region over the past decade, but very little has been done to study the drivers of waterborne diseases in the region. We investigated the relationship between water, sanitation, and hygiene (WASH) parameters, microbial and antibiotic resistance (AR) contamination levels in drinking water, and health outcomes using health survey and molecular analysis during June and July of 2014 in two settlement types (agro-pastoralist villages and transhumant pastoralist camps)...
April 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28425478/data-intensive-genome-level-analysis-for-identifying-novel-non-toxic-drug-targets-for-multi-drug-resistant-mycobacterium-tuberculosis
#16
Divneet Kaur, Rintu Kutum, Debasis Dash, Samir K Brahmachari
We report the construction of a novel Systems Biology based virtual drug discovery model for the prediction of non-toxic metabolic targets in Mycobacterium tuberculosis (Mtb). This is based on a data-intensive genome level analysis and the principle of conservation of the evolutionarily important genes. In the 1623 sequenced Mtb strains, 890 metabolic genes identified through a systems approach in Mtb were evaluated for non-synonymous mutations. The 33 genes showed none or one variation in the entire 1623 strains, including 1084 Russian MDR strains...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425271/molecular-characteristics-and-antibiotic-resistance-of-acinetobacter-baumanniibeta-lactamase-producing-isolates-a-predominance-of-intrinsic-blaoxa-51-and-detection-of-tem-and-ctx-m-genes
#17
Amir Ibrahimagić, Farah Kamberović, Selma Uzunović, Branka Bedenić, Emina Idrizović
BACKGROUND/AIM: The aim of this study was to determine the molecular characteristics and antibiotic resistance of 13 (10 inpatient and three outpatient) Acinetobacter baumannii beta-lactamase-producing isolates collected in Bosnia and Herzegovina between December 2009 and May 2010. MATERIALS AND METHODS: Susceptibility testing was performed by disk diffusion and broth microdilution methods. The modified Hodge and combined disk test with EDTA/phenylboronic acid was used to screen for carbapenemase production...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28422755/%C3%AE-klotho-deficiency-protects-against-obesity-through-a-crosstalk-between-liver-microbiota-and-brown-adipose-tissue
#18
Emmanuel Somm, Hugues Henry, Stephen J Bruce, Sébastien Aeby, Marta Rosikiewicz, Gerasimos P Sykiotis, Mohammed Asrih, François R Jornayvaz, Pierre Damien Denechaud, Urs Albrecht, Moosa Mohammadi, Andrew Dwyer, James S Acierno, Kristina Schoonjans, Lluis Fajas, Gilbert Greub, Nelly Pitteloud
β-Klotho (encoded by Klb) is the obligate coreceptor mediating FGF21 and FGF15/19 signaling. Klb-/- mice are refractory to beneficial action of pharmacological FGF21 treatment including stimulation of glucose utilization and thermogenesis. Here, we investigated the energy homeostasis in Klb-/- mice on high-fat diet in order to better understand the consequences of abrogating both endogenous FGF15/19 and FGF21 signaling during caloric overload. Surprisingly, Klb-/- mice are resistant to diet-induced obesity (DIO) owing to enhanced energy expenditure and BAT activity...
April 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28422476/origin-dependent-variations-in-the-atmospheric-microbiome-community-in-eastern-mediterranean-dust-storms
#19
Daniella Gat, Yinon Mazar, Eddie Cytryn, Yinon Rudich
Dust storms transport through the atmosphere microorganisms that can potentially affect human health and the functionality of microbial communities in various environments. Characterizing the dust-borne microbiome in storms of different origin or that followed different paths provides valuable data to improve understanding of global health and environmental impacts. We present a comparative study on the diversity of dust-borne microbial communities in dust storms from three distinct origins-North Africa, Syria and Saudi-Arabia-and compare them with those on dust-free days...
April 19, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28421718/small-serine-recombination-systems-para-mrs-and-cinh-rs2-perform-precise-excision-of-plastid-dna
#20
Min Shao, Ann Blechl, James G Thomson
Selectable marker genes (SMGs) are necessary for selection of transgenic plants. However, once stable transformants have been identified, the marker gene is no longer needed. In this study, we demonstrate the use of the small serine recombination systems, ParA-MRS and CinH-RS2, to precisely excise a marker gene from the plastid genome of tobacco. Transplastomic plants transformed with the pTCH-MRS and pTCH-RS2 vectors, containing the visual reporter gene DsRed flanked by directly oriented MRS and RS2 recognition sites, respectively, were crossed with nuclear-genome transformed tobacco plants expressing plastid-targeted ParA and CinH recombinases, respectively...
April 19, 2017: Plant Biotechnology Journal
keyword
keyword
109798
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"