keyword
https://read.qxmd.com/read/38144376/in-silico-screening-and-molecular-dynamics-simulation-of-potential-anti-malarial-agents-from-zingiberaceae-as-potential-plasmodium-falciparum-lactate-dehydrogenase-pfldh-enzyme-inhibitors
#21
JOURNAL ARTICLE
Muhammad Fikri Heikal, Wira Eka Putra, Sustiprijatno, Muhaimin Rifa'i, Arief Hidayatullah, Febby Nurdiya Ningsih, Diana Widiastuti, Adawiyah Suriza Shuib, Baiq Feby Zulfiani, Afrabias Firyal Hanasepti
Malaria continues to be a major public health issue in a number of countries, particularly in tropical regions-the emergence of drug-resistant Plasmodium falciparum encourages new drug discovery research. The key to Plasmodium falciparum survival is energy production up to 100 times greater than other parasites, primarily via the PfLDH. This study targets PfLDH with natural bioactive compounds from the Zingiberaceae family through molecular docking and molecular dynamic studies. Sulcanal, quercetin, shogosulfonic acid C, galanal A and naringenin are the Top 5 compounds with a lower binding energy value than chloroquine, which was used as a control in this study...
June 2023: Tropical Life Sciences Research
https://read.qxmd.com/read/38101411/pyruvate-modulation-of-redox-potential-controls-mouse-sperm-motility
#22
JOURNAL ARTICLE
Cameron A Schmidt, Benjamin J Hale, Debajit Bhowmick, William J Miller, P Darrell Neufer, Christopher B Geyer
Sperm gain fertilization competence in the female reproductive tract through a series of biochemical changes and a requisite switch from linear progressive to hyperactive motility. Despite being essential for fertilization, regulation of sperm energy transduction is poorly understood. This knowledge gap confounds interpretation of interspecies variation and limits progress in optimizing sperm selection for assisted reproduction. Here, we developed a model of mouse sperm bioenergetics using metabolic phenotyping data, quantitative microscopy, and spectral flow cytometry...
December 5, 2023: Developmental Cell
https://read.qxmd.com/read/38084815/a-vesicular-warburg-effect-aerobic-glycolysis-occurs-on-axonal-vesicles-for-local-nad-recycling-and-transport
#23
JOURNAL ARTICLE
Maximilian Mc Cluskey, Hervé Dubouchaud, Anne-Sophie Nicot, Frédéric Saudou
In neurons, fast axonal transport (FAT) of vesicles occurs over long distances and requires constant and local energy supply for molecular motors in the form of adenosine triphosphate (ATP). FAT is independent of mitochondrial metabolism. Indeed, the glycolytic machinery is present on vesicles and locally produces ATP, as well as nicotinamide adenine dinucleotide bonded with hydrogen (NADH) and pyruvate, using glucose as a substrate. It remains unclear whether pyruvate is transferred to mitochondria from the vesicles as well as how NADH is recycled into NAD+ on vesicles for continuous glycolysis activity...
December 12, 2023: Traffic
https://read.qxmd.com/read/38077825/platelets-of-covid-19-patients-display-mitochondrial-dysfunction-oxidative-stress-and-energy-metabolism-failure-compatible-with-cell-death
#24
JOURNAL ARTICLE
Valentine Léopold, Osoul Chouchane, Joe M Butler, Alex R Schuurman, Erik H A Michels, Justin de Brabander, Bauke V Schomakers, Michel van Weeghel, Daisy I Picavet-Havik, Anita E Grootemaat, Renée A Douma, Tom D Y Reijnders, Augustijn M Klarenbeek, Brent Appelman, W Joost Wiersinga, Nicole N van der Wel, Jeroen den Dunnen, Riekelt H Houtkooper, Cornelis Van't Veer, Tom van der Poll
BACKGROUND: Alterations in platelet function have been implicated in the pathophysiology of COVID-19 since the beginning of the pandemic. While early reports linked hyperactivated platelets to thromboembolic events in COVID-19, subsequent investigations demonstrated hyporeactive platelets with a procoagulant phenotype. Mitochondria are important for energy metabolism and the function of platelets. OBJECTIVES: Here, we sought to map the energy metabolism of platelets in a cohort of noncritically ill COVID-19 patients and assess platelet mitochondrial function, activation status, and responsiveness to external stimuli...
October 2023: Research and Practice in Thrombosis and Haemostasis
https://read.qxmd.com/read/38071053/-levels-and-clinical-significances-of-spd-1-and-spd-l1-in-peripheral-blood-of-lymphoma-patients
#25
JOURNAL ARTICLE
Xiao-Liu Liu, Ping He, Jian Lei, Li-Xin Zou, Lang Peng
OBJECTIVE: To observe the levels of soluble programmed cell death protein 1 (sPD-1) and soluble programmed cell death ligand 1 (sPD-L1) in peripheral blood of lymphoma patients, and reveal their clinical significances. METHODS: The peripheral blood specimens and clinical data of 64 newly diagnosed lymphoma patients and 30 healthy volunteers were collected. The levels of sPD-1 and sPD-L1 were detected by enzyme-linked immunosorbent assay (ELISA), and their correlations with clinical characteristics of the patients including pathological type, stage, lactate dehydrogenase (LDH) level, T cell subsets were analyzed...
December 2023: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://read.qxmd.com/read/38069233/biological-impact-of-organic-extracts-from-urban-air-particulate-matter-an-in-vitro-study-of-cytotoxic-and-metabolic-effects-in-lung-cells
#26
JOURNAL ARTICLE
Tatiana D Silva, Célia Alves, Helena Oliveira, Iola F Duarte
Atmospheric particulate matter (PM) with diameters below 10 µm (PM10 ) may enter the lungs through inhalation and are linked to various negative health consequences. Emergent evidence emphasizes the significance of cell metabolism as a sensitive target of PM exposure. However, the current understanding of the relationship between PM composition, conventional toxicity measures, and the rewiring of intracellular metabolic processes remains limited. In this work, PM10 sampled at a residential area (urban background, UB) and a traffic-impacted location (roadside, RS) of a Portuguese city was comprehensively characterized in terms of polycyclic aromatic hydrocarbons and plasticizers...
November 29, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38066993/lactate-dehydrogenase-gene-family-in-spirometra-mansoni-cestoda-diphyllobothriidea-phylogenetic-patterns-and-molecular-characteristics
#27
JOURNAL ARTICLE
Shasha Liu, Ke Zhou, Fei Gao, Wen Li, Zhongquan Wang, Xi Zhang
The plerocercoid of Spirometra mansoni can parasitize both human and animals, resulting in sparganosis. Lactate dehydrogenase (LDH) is an important enzyme in parasites. However, our knowledge of the LDH family in S. mansoni is still inadequate. This work identified 19 new LDH members in S. mansoni . Clustering analysis demonstrated that all Sm LDHs were divided into two main groups, which is consistent with the patterns of conserved motif organization. According to RT-qPCR, 2 LDHs were highly expressed in the plerocercoid stage and 17 LDHs were highly expressed in the adult stage...
November 24, 2023: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38063074/sensitive-monitoring-of-nad-p-h-levels-within-cancer-cells-using-mitochondria-targeted-near-infrared-cyanine-dyes-with-optimized-electron-withdrawing-acceptors
#28
JOURNAL ARTICLE
Dilka Liyana Arachchige, Sushil K Dwivedi, May Waters, Sophia Jaeger, Joe Peters, Daniel R Tucker, Micaela Geborkoff, Thomas Werner, Rudy L Luck, Bhaskar Godugu, Haiying Liu
A series of near-infrared fluorescent probes, labeled A to E, were developed by combining electron-rich thiophene and 3,4-ethylenedioxythiophene bridges with 3-quinolinium and various electron deficient groups, enabling the sensing of NAD(P)H. Probes A and B exhibit absorptions and emissions in the near-infrared range, offering advantages such as minimal interference from autofluorescence, negligible photo impairment in cells and tissues, and exceptional tissue penetration. These probes show negligible fluorescence when NADH is not present, and their absorption maxima are at 438 nm and 470 nm, respectively...
December 8, 2023: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://read.qxmd.com/read/38057866/nicotinamide-mononucleotide-as-a-therapeutic-agent-to-alleviate-multi-organ-failure-in-sepsis
#29
JOURNAL ARTICLE
Ting Cao, Rui Ni, Weimin Ding, Xiaoyun Ji, Guo-Chang Fan, Zhuxu Zhang, Tianqing Peng
BACKGROUND: Sepsis-caused multi-organ failure remains the major cause of morbidity and mortality in intensive care units with limited therapeutics. Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD+ ), has been recently reported to be protective in sepsis; however, its therapeutic effects remain to be determined. This study sought to investigate the therapeutic effects of NMN in septic organ failure and its underlying mechanisms. METHODS: Sepsis was induced by feces-injection-in-peritoneum in mice...
December 6, 2023: Journal of Translational Medicine
https://read.qxmd.com/read/38054548/metabolic-alterations-unravel-the-maternofetal-immune-responses-with-disease-severity-in-pregnant-women-infected-with-sars-cov-2
#30
JOURNAL ARTICLE
Sandhya Hora, Prabhjyoti Pahwa, Hamda Siddiqui, Anoushka Saxena, Minal Kashyap, Jayesh K Sevak, Ravinder Singh, Maryam Javed, Pushpa Yadav, Pratibha Kale, Gayatri Ramakrishna, Meenu Bajpai, Asmita Rathore, Jaswinder S Maras, Shakun Tyagi, Shiv K Sarin, Nirupama Trehanpati
Pregnancy being an immune compromised state, coronavirus disease of 2019 (COVID-19) disease poses high risk of premature delivery and threat to fetus. Plasma metabolome regulates immune cellular responses, therefore we aimed to analyze the change in plasma secretome, metabolome, and immune cells with disease severity in COVID-19 positive pregnant females and their cord blood. COVID-19 reverse transcriptase-polymerase chain reaction positive pregnant females (n = 112) with asymptomatic (Asy) (n = 82), mild (n = 21), or moderate (n = 9) disease, healthy pregnant (n = 18), COVID-19 positive nonpregnant females (n = 7) were included...
December 2023: Journal of Medical Virology
https://read.qxmd.com/read/38041502/aging-of-stallion-spermatozoa-stored-in-vitro-is-delayed-at-22%C3%A2-c-using-a-67%C3%A2-mm-glucose-10%C3%A2-mm-pyruvate-based-media
#31
JOURNAL ARTICLE
Laura Becerro-Rey, Francisco Eduardo Martín-Cano, Cristina Ortega Ferrusola, Heriberto Rodríguez-Martínez, Gemma Gaitskell-Phillips, Eva da Silva-Álvarez, Antonio Silva-Rodríguez, María Cruz Gil, Fernando J Peña
BACKGROUND: Most commerce of equine seminal doses is carried out using commercial extenders under refrigeration at 5°C. OBJECTIVES: To determine if 10 mm pyruvate in a 67 mm glucose extender and storage at 22°C could be the basis of an alternative storage method to cooling to 5°C. MATERIAL AND METHODS: Stallion ejaculates were extendedin: INRA96 (67 mm glucose, non-pyruvate control), modified Tyrode's (67 mm glucose-10 mm pyruvate), supplemented with 0, 10, 50, and 100 μM itaconate...
December 1, 2023: Andrology
https://read.qxmd.com/read/38039759/cryptotanshinone-suppresses-ovarian-cancer-via-simultaneous-inhibition-of-glycolysis-and-oxidative-phosphorylation
#32
JOURNAL ARTICLE
Tong Wang, Mengmeng Zhang, Muhammad Khan, Jingjing Li, Xiao Wu, Tonghui Ma, Yongming Li
Ovarian cancer is one of the most lethal cancers in female reproductive system due to heterogeneity and lack of effective treatment. Targeting aerobic glycolysis, a predominant energy metabolism of cancer cells has been recognized a novel strategy to overcome cancer cell growth. However, the capability of cancer cells to undergo metabolic reprogramming guarantees their survival even when glycolysis is inhibited. Here in this study, we have shown that Cryptotanshinone (CT), a lipid-soluble bioactive anticancer molecule of Salvia miltiorrhiza, inhibits both glycolysis and oxidative phosphorylation (OXPHOS) in ovarian cancer cells leading to growth suppression and apoptosis induction...
November 30, 2023: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38036520/the-mitochondrial-nadh-shuttle-system-is-a-targetable-vulnerability-for-group-3-medulloblastoma-in-a-hypoxic-microenvironment
#33
JOURNAL ARTICLE
J Contenti, Y Guo, A Mazzu, M Irondelle, M Rouleau, C Lago, G Leva, L Tiberi, I Ben-Sahra, F Bost, N M Mazure
Medulloblastoma is a cancerous brain tumor that affects mostly children. Among the four groups defined by molecular characteristics, Group 3, the least well characterized, is also the least favorable, with a survival rate of 50%. Current treatments, based on surgery, radiotherapy, and chemotherapy, are not adequate and the lack of understanding of the different molecular features of Group 3 tumor cells makes the development of effective therapies challenging. In this study, the problem of medulloblastoma is approached from a metabolic standpoint in a low oxygen microenvironment...
November 30, 2023: Cell Death & Disease
https://read.qxmd.com/read/38029686/pyruvate-enhances-stallion-sperm-function-in-high-glucose-media-improving-overall-metabolic-efficiency
#34
JOURNAL ARTICLE
Francisco E Martín-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodríguez, Cristina Ortega-Ferrusola, María Cruz Gil, Fernando J Peña
If a mechanism of more efficient glycolysis depending on pyruvate is present in stallion spermatozoa, detrimental effects of higher glucose concentrations that are common in current commercial extenders could be counteracted. To test this hypothesis, spermatozoa were incubated in a 67 mM Glucose modified Tyrode's media in the presence of 1- or 10-mM pyruvate and in the Tyrode's basal media which contains 5 mM glucose. Spermatozoa incubated for 3 h at 37 °C in 67 mM Tyrode's media with 10 mM pyruvate showed increased motility in comparison with aliquots incubated in Tyrode's 5 mM glucose and Tyrode's 67 mM glucose (57...
November 23, 2023: Theriogenology
https://read.qxmd.com/read/38028938/lipoaspirate-stored-at-a-constant-low-temperature-by-electric-control-suppresses-intracellular-metabolism-and-maintains-high-cell-viability
#35
JOURNAL ARTICLE
Ryoko Inaki, Yoshihiko Sato, Daisuke Nakamura, Yoshiyuki Aikawa, Tsuyoshi Takato, Kazuto Hoshi, Atsuhiko Hikita
BACKGROUND: Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as temperature, time, air pressure, and vibration affect the cell functions isolated from body tissues. In particular, temperature is a critical factor which determines the viability of the cells and organs...
December 2023: Regenerative Therapy
https://read.qxmd.com/read/38028810/effect-of-circadian-rhythm-on-nad-and-other-metabolites-in-human-brain
#36
JOURNAL ARTICLE
Bernard Cuenoud, Zhiwei Huang, Mickael Hartweg, Mark Widmaier, SongI Lim, Daniel Wenz, Lijing Xin
Nicotinamide Adenine Dinucleotide (NAD) plays a central role in the master circadian clock of the brain (the suprachiasmatic nuclei, SCN) as demonstrated in many model organisms. NAD acts as an enzyme co-factor and substrate and its modulation was found to be tightly regulated to the periodicity of the cycles. However, in human brain, the effect of the circadian rhythm (CR) on the metabolism of the SCN and other brain regions is poorly understood. We conducted a magnetic resonance spectroscopy (MRS) study at a high magnetic field, measuring the occipital brain NAD levels and other metabolites in two different morning and afternoon diurnal states in 25 healthy participants...
2023: Frontiers in Physiology
https://read.qxmd.com/read/38003412/plant-life-with-and-without-oxygen-a-metabolomics-approach
#37
REVIEW
Vladislav V Yemelyanov, Roman K Puzanskiy, Maria F Shishova
Oxygen deficiency is an environmental challenge which affects plant growth, the development and distribution in land and aquatic ecosystems, as well as crop yield losses worldwide. The capacity to exist in the conditions of deficiency or the complete lack of oxygen depends on a number of anatomic, developmental and molecular adaptations. The lack of molecular oxygen leads to an inhibition of aerobic respiration, which causes energy starvation and the acceleration of glycolysis passing into fermentations. We focus on systemic metabolic alterations revealed with the different approaches of metabolomics...
November 12, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37957679/autofluorescence-imaging-of-endogenous-metabolic-cofactors-in-response-to-cytokine-stimulation-of-classically-activated-macrophages
#38
JOURNAL ARTICLE
Shelby N Bess, Matthew J Igoe, Abby C Denison, Timothy J Muldoon
BACKGROUND: Macrophages are one of the most prevalent subsets of immune cells within the tumor microenvironment and perform a range of functions depending on the cytokines and chemokines released by surrounding cells and tissues. Recent research has revealed that macrophages can exhibit a spectrum of phenotypes, making them highly plastic due to their ability to alter their physiology in response to environmental cues. Recent advances in examining heterogeneous macrophage populations include optical metabolic imaging, such as fluorescence lifetime imaging (FLIM), and multiphoton microscopy...
November 13, 2023: Cancer & Metabolism
https://read.qxmd.com/read/37896171/targeting-lactate-dehydrogenase-b-as-a-strategy-to-fight-cancer-identification-of-potential-inhibitors-by-in-silico-analysis-and-in-vitro-screening
#39
JOURNAL ARTICLE
Manos Vlasiou, Vicky Nicolaidou, Christos Papaneophytou
Lactate dehydrogenase (LDH) is an enzyme that catalyzes the reversible conversion of lactate to pyruvate while reducing NAD+ to NADH (or oxidizing NADH to NAD+ ). Due to its central role in the Warburg effect, LDH-A isoform has been considered a promising target for treating several types of cancer. However, research on inhibitors targeting LDH-B isoform is still limited, despite the enzyme's implication in the development of specific cancer types such as breast and lung cancer. This study aimed to identify small-molecule compounds that specifically inhibit LDH-B...
October 1, 2023: Pharmaceutics
https://read.qxmd.com/read/37817572/lactate-induces-the-development-of-beige-adipocytes-via-an-increase-in-the-level-of-reactive-oxygen-species
#40
JOURNAL ARTICLE
Nana Esaki, Toshiro Matsui, Takanori Tsuda
Recent studies have indicated that lactate acts as a signaling molecule in various tissues. We previously demonstrated that intake of an amino acid mixture combined with exercise synergistically induced beige adipocyte formation in inguinal white adipose tissue (iWAT) in mice. Moreover, plasma lactate levels remained significantly elevated in the amino acid mixture + exercise group even 16 h after exercise, indicating that a lactate-mediated pathway may be involved in the induction of beige adipocyte formation...
October 11, 2023: Food & Function
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