keyword
MENU ▼
Read by QxMD icon Read
search

Nkcc

keyword
https://www.readbyqxmd.com/read/30012538/helicokinin-alters-ion-transport-in-the-secondary-cell-containing-region-of-the-malpighian-tubule-of-the-larval-cabbage-looper-trichoplusia-ni
#1
Dennis Kolosov, Michael J O'Donnell
Excretion in insects is accomplished by the combined actions of the Malpighian tubules (MTs) and hindgut, which together form the functional kidney. MTs of many insect groups consist of principal cells (PC) and secondary cells (SC). In most insect groups SCs are reported to secrete ions from haemolymph into the tubule lumen. Paradoxically, SCs in the MTs of the lepidopteran cabbage looper T. ni are used to reabsorb Na+ and K+ back into haemolymph. The current study was designed to investigate the effects and mode of action of the lepidopteran kinin, Helicokinin (HK), on ion transport in the SC-containing region of MT of T...
July 14, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29923766/wnk-spak-osr1-signaling-lessons-learned-from-an-insect-renal-epithelium
#2
Aylin R Rodan
WNK (With No Lysine (K)) kinases regulate renal epithelial ion transport to maintain homeostasis of electrolyte concentrations, extracellular volume and blood pressure. The SLC12 cation-chloride cotransporters, including the sodium-potassium-2-chloride (NKCC) and sodium-chloride (NCC) cotransporters, are targets of WNK regulation via the intermediary kinases SPAK (Ste20-related proline/alanine-rich kinase) and OSR1 (oxidative stress response). The pathway is activated by low dietary potassium intake, resulting in increased phosphorylation and activity of NCC...
June 20, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29908900/molecular-mechanisms-of-bi-directional-ion-transport-in-the-malpighian-tubules-of-a-lepidopteran-crop-pest-trichoplusia-ni
#3
Dennis Kolosov, Maria Tauqir, Sabitha Rajaruban, Peter M Piermarini, Andrew Donini, Michael J O'Donnell
Classical studies have described in detail the complex and regionalized morphology of the Malpighian tubule (MT) in larval Lepidoptera. Recent studies revealed unusual aspects of ion transport in the Malpighian tubules of the larva of the cabbage looper, Trichoplusia ni. These included: cation reabsorption via secondary cells (SC); coupling of SCs to neighbouring PCs via gap junctions to enable reabsorption; and a reversal from cation secretion to reabsorption by the principal cells in the distal ileac plexus region of the in situ tubule in response to dietary ion loading...
June 14, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29897966/salinity-reduction-benefits-european-eel-larvae-insights-at-the-morphological-and-molecular-level
#4
Sebastian N Politis, David Mazurais, Arianna Servili, Jose-Luis Zambonino-Infante, Joanna J Miest, Jonna Tomkiewicz, Ian A E Butts
European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions...
2018: PloS One
https://www.readbyqxmd.com/read/29874916/sodium-transporters-are-involved-in-lithium-influx-in-brain-endothelial-cells
#5
Huilong Luo, Matthieu Gauthier, Xi Tan, Christophe Landry, Joël Poupon, Marie-Pierre Dehouck, Fabien Gosselet, Nicolas Perrière, Frank Bellivier, Salvatore Cisternino, Xavier Declèves
Variability in drug response to lithium (Li+ ) is poorly understood and significant, as only 40% of patients with bipolar disorder highly respond to Li+ . Li+ can be transported by sodium (Na+ ) transporters in kidney tubules or red blood cells, but its transport has not been investigated at the blood-brain barrier (BBB). Inhibition and/or transcriptomic strategies for Na+ transporters such as NHE (SLC9), NBC (SLC4), and NKCC (SLC12) were used to assess their role on Li+ transport in human brain endothelial cells...
July 2, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29783866/effect-of-the-flame-retardant-tris-1-3-dichloro-2-propyl-phosphate-tdcpp-on-na-k-atpase-and-cl-transport-in-hela-cells
#6
Simona Latronico, Maria Elena Giordano, Emanuela Urso, Maria Giulia Lionetto, Trifone Schettino
Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) is one of the most diffused phosphorus flame retardants in the environment and is highly persistent and abundant in residential dust samples. To date the cellular targets and mechanisms underlying its toxic effects are not completely understood. The aim of this work was to study the effects of TDCPP on ion transport mechanisms fundamental for the cellular ionic homeostasis, such as Na+ -K+ -ATPase and Cl- transport. HeLa cells were used as experimental model. TDCPP showed a dose-dependent effect on cell viability in cells exposed for 24 h as assessed by MTT test (IC50  = 52...
June 26, 2018: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29739261/exacerbated-brain-edema-in-a-rat-streptozotocin-model-of-hyperglycemic-ischemic-stroke-evidence-for-involvement-of-blood-brain-barrier-na-k-cl-cotransport-and-na-h-exchange
#7
Natalie Y Yuen, Olga V Chechneva, Yi-Je Chen, Yi-Chen Tsai, Logan K Little, James Dang, Daniel J Tancredi, Jacob Conston, Steven E Anderson, Martha E O'Donnell
Cerebral edema is exacerbated in diabetic ischemic stroke through poorly understood mechanisms. We showed previously that blood-brain barrier (BBB) Na-K-Cl cotransport (NKCC) and Na/H exchange (NHE) are major contributors to edema formation in normoglycemic ischemic stroke. Here, we investigated whether hyperglycemia-exacerbated edema involves changes in BBB NKCC and NHE expression and/or activity and whether inhibition of NKCC or NHE effectively reduces edema and injury in a type I diabetic model of hyperglycemic stroke...
January 1, 2018: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29705850/psychoneuroimmunology-and-natural-killer-cells-the-chromium-release-whole-blood-assay
#8
Mary Ann Fletcher, Zachary Barnes, Gordon Broderick, Nancy G Klimas
Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter describes a chromium (51 Cr)-release bioassay designed to measure to the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29687903/na-k-2cl-cotransporter-nkcc-physiological-function-in-nonpolarized-cells-and-transporting-epithelia
#9
Eric Delpire, Kenneth B Gagnon
Two genes encode the Na+ -K+ -2Cl- cotransporters, NKCC1 and NKCC2, that mediate the tightly coupled movement of 1Na+ , 1K+ , and 2Cl- across the plasma membrane of cells. Na+ -K+ -2Cl- cotransport is driven by the chemical gradient of the three ionic species across the membrane, two of them maintained by the action of the Na+ /K+ pump. In many cells, NKCC1 accumulates Cl- above its electrochemical potential equilibrium, thereby facilitating Cl- channel-mediated membrane depolarization. In smooth muscle cells, this depolarization facilitates the opening of voltage-sensitive Ca2+ channels, leading to Ca2+ influx, and cell contraction...
March 25, 2018: Comprehensive Physiology
https://www.readbyqxmd.com/read/29204056/effects-of-hemp-seed-soft-capsule-on-colonic-ion-transport-in-rats
#10
Xiao-Fang Lu, Meng-Di Jia, Sheng-Sheng Zhang, Lu-Qing Zhao
AIM: To investigate the effect of Hemp seed soft capsule (HSCC) on colonic ion transport and its related mechanisms in constipation rats. METHODS: Sprague-Dawley male rats were randomly divided into three groups: normal group, constipation group and HSSC group. Rats in the constipation and HSSC groups were administrated loperamide 3 mg/kg per day orally for 12 d to induce the constipation model. Then, the HSSC group was given HSSC 0.126 g/kg per day by gavage for 7 d...
November 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29150743/differential-response-of-neural-cells-to-trauma-induced-swelling-in-vitro
#11
A R Jayakumar, M Taherian, K S Panickar, N Shamaladevi, M E Rodriguez, B G Price, M D Norenberg
Brain edema and the associated increase in intracranial pressure are major consequences of traumatic brain injury (TBI) that accounts for most early deaths after TBI. We recently showed that acute severe trauma to cultured astrocytes results in cell swelling. We further examined whether trauma induces cell swelling in neurons and microglia. We found that severe trauma also caused cell swelling in cultured neurons, whereas no swelling was observed in microglia. While severe trauma caused cell swelling in both astrocytes and neurons, mild trauma to astrocytes, neurons, and microglia failed to cell swelling...
February 2018: Neurochemical Research
https://www.readbyqxmd.com/read/28924368/the-effects-of-perioperative-anesthesia-and-analgesia-on-immune-function-in-patients-undergoing-breast-cancer-resection-a-prospective-randomized-study
#12
RANDOMIZED CONTROLLED TRIAL
Jin Sun Cho, Mi-Hyang Lee, Seung Il Kim, Seho Park, Hyung Seok Park, Ein Oh, Jong Ho Lee, Bon-Nyeo Koo
Introduction: Perioperative anesthesia and analgesia exacerbate immunosuppression in immunocompromised cancer patients. The natural killer (NK) cell is a critical part of anti-tumor immunity. We compared the effects of two different anesthesia and analgesia methods on the NK cell cytotoxicity (NKCC) in patients undergoing breast cancer surgery. Methods: Fifty patients undergoing breast cancer resection were randomly assigned to receive propofol-remifentanil anesthesia with postoperative ketorolac analgesia (Propofol-ketorolac groups) or sevoflurane-remifentanil anesthesia with postoperative fentanyl analgesia (Sevoflurane-fentanyl group)...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28923771/differential-expression-of-putative-sodium-dependent-cation-chloride-cotransporters-in-aedes-aegypti
#13
COMPARATIVE STUDY
Peter M Piermarini, Daniel C Akuma, John C Crow, Taylor L Jamil, Willa G Kerkhoff, Kenyatta C M F Viel, Christopher M Gillen
The yellow fever mosquito, Aedes aegypti, has three genes that code for proteins with sequence similarity to vertebrate Na+ -K+ -Cl- cotransporters (NKCCs) of the solute-linked carrier 12 superfamily of cation-chloride cotransporters (CCCs). We hypothesized that these mosquito NKCC orthologues have diverged to perform distinct roles in salt secretion and absorption. In phylogenetic analyses, one protein (aeNKCC1) groups with a Drosophila melanogaster NKCC that mediates salt secretion whereas two others (aeCCC2 and aeCCC3) group with a Drosophila transporter that is not functionally characterized...
December 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28852583/comparative-molecular-analyses-of-select-ph-and-osmoregulatory-genes-in-three-freshwater-crayfish-cherax-quadricarinatus-c-destructor-and-c-cainii
#14
Muhammad Y Ali, Ana Pavasovic, Lalith K Dammannagoda, Peter B Mather, Peter J Prentis
Systemic acid-base balance and osmotic/ionic regulation in decapod crustaceans are in part maintained by a set of transport-related enzymes such as carbonic anhydrase (CA), Na(+)/K(+)-ATPase (NKA), H(+)-ATPase (HAT), Na(+)/K(+)/2Cl(-) cotransporter (NKCC), Na(+)/Cl(-)/HCO[Formula: see text] cotransporter (NBC), Na(+)/H(+) exchanger (NHE), Arginine kinase (AK), Sarcoplasmic Ca(+2)-ATPase (SERCA) and Calreticulin (CRT). We carried out a comparative molecular analysis of these genes in three commercially important yet eco-physiologically distinct freshwater crayfish, Cherax quadricarinatus, C...
2017: PeerJ
https://www.readbyqxmd.com/read/28829424/a-flow-cytometry-based-cytotoxicity-assay-for-the-assessment-of-human-nk-cell-activity
#15
Fadi Kandarian, Gemalene M Sunga, Diana Arango-Saenz, Maura Rossetti
Within the innate immune system, effector lymphocytes known as natural killer (NK) cells play an essential role in host defense against aberrant cells, specifically eliminating tumoral and virally infected cells. Approximately 30 known monogenic defects, together with a host of other pathological conditions, cause either functional or classic NK cell deficiency, manifesting in reduced or absent cytotoxic activity. Historically, cytotoxicity has been investigated with radioactive methods, which are cumbersome, expensive and potentially hazardous...
August 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28818393/the-modulation-of-the-phosphorylation-status-of-nkcc1-in-organ-cultured-bovine-lenses-implications-for-the-regulation-of-fiber-cell-and-overall-lens-volume
#16
Irene Vorontsova, Paul J Donaldson, Zhiying Kong, Chiharu Wickremesinghe, Leo Lam, Julie C Lim
In previous work, we have shown the Sodium/Potassium/2 Chloride Cotransporter (NKCC1) to be a key effector of lens fiber cell volume regulation. Since others have shown that the activity of NKCC1 is regulated via its phosphorylation status, the purpose of this study was to investigate whether NKCC1 phosphorylation can be modulated in organ cultured bovine lenses, and to see how this relates to changes in lens wet weight. Western blotting was first used to confirm the expression of NKCC1, phosphorylated NKCC1 (NKCC1-P) and the regulatory kinases WNK/SPAK and phosphatases PP1/PP2A in bovine lenses at the protein level...
December 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28522431/wnk1-and-p38-mapk-distribution-in-ionocytes-and-accessory-cells-of-euryhaline-teleost-fish-implies-ionoregulatory-function
#17
W S Marshall, R R F Cozzi, M Spieker
Ionocytes of euryhaline teleost fish secrete NaCl, under regulation by serine and threonine kinases, including with-no-lysine kinase (WNK1) and p38 mitogen-activated protein kinase (MAPK). Mummichogs (Fundulus heteroclitus L.) were acclimated to freshwater (FW), full strength seawater (SW) and hypersaline conditions (2SW). Immunocytochemistry of ionocytes in opercular epithelia of fish acclimated to SW and 2SW revealed that WNK1-anti-pT58 phosphoantibody localized strongly to accessory cells and was present in the cytosol of ionocytes, close to cystic fibrosis transmembrane conductance regulator (CFTR) in the apical membrane and the sodium potassium 2 chloride cotransporter (NKCC) in the basolateral membrane...
July 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28482023/understanding-the-genomic-basis-of-adaptive-response-to-variable-osmotic-niches-in-freshwater-prawns-a-comparative-intraspecific-rna-seq-analysis-of-macrobrachium-australiense
#18
Azam Moshtaghi, Md Lifat Rahi, Peter B Mather, David A Hurwood
Understanding the molecular basis of adaptive response to variable environmental conditions is a central goal of evolutionary biology. Here, we sought to identify potential outlier single nucleotide polymorphisms (SNPs) in 3 wild populations of a freshwater prawn (Macrobrachium australiense) that are exposed to differing osmotic niches by using a comparative transcriptomics approach. De novo assembly of approximately 542 million (75 nt) pair end reads collected from 10 individuals revealed 123396 longer contigs/transcripts of variable length, that showed 97...
July 1, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28408927/activities-of-amphioxus-gh-like-protein-in-osmoregulation-insight-into-origin-of-vertebrate-gh-family
#19
Mengyang Li, Chengyan Jiang, Yu Zhang, Shicui Zhang
GH is known to play an important role in both growth promotion and osmoregulation in vertebrates. We have shown that amphioxus possesses a single GH-like hormone (GHl) gene encoding a functional protein capable of promoting growth. However, if GHl can mediate osmoregulation remains open. Here, we demonstrated clearly that GHl increased not only the survival rate of amphioxus but also the muscle moisture under high salinity. Moreover, GHl induced the expression of both the ion transporter Na(+)-K(+)-ATPase (NKA) and Na(+)-K(+)-2Cl(-) cotransporter (NKCC) in the gill as well as the mediator of GH action IGFl in the hepatic caecum, indicating that GHl fulfills this osmoregulatory activity through the same mechanisms of vertebrate GH...
2017: International Journal of Endocrinology
https://www.readbyqxmd.com/read/28223394/primary-adrenal-insufficiency-is-associated-with-impaired-natural-killer-cell-function-a-potential-link-to-increased-mortality
#20
Irina Bancos, Jon Hazeldine, Vasileios Chortis, Peter Hampson, Angela E Taylor, Janet M Lord, Wiebke Arlt
OBJECTIVE: Mortality in patients with primary adrenal insufficiency (PAI) is significantly increased, with respiratory infections as a major cause of death. Moreover, patients with PAI report an increased rate of non-fatal infections. Neutrophils and natural killer (NK) cells are innate immune cells that provide frontline protection against invading pathogens. Thus, we compared the function and phenotype of NK cells and neutrophils isolated from PAI patients and healthy controls to ascertain whether altered innate immune responses could be a contributory factor for the increased susceptibility of PAI patients to infection...
April 2017: European Journal of Endocrinology
keyword
keyword
109741
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"