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https://www.readbyqxmd.com/read/29784673/formation-of-the-sacrum-requires-down-regulation-of-sonic-hedgehog-signaling-in-the-sacral-intervertebral-discs
#1
Raffaella Bonavita, Kathleen Vincent, Robert Pinelli, Chitra Lekha Dahia
In humans, the sacrum forms an important component of the pelvic arch, and it transfers the weight of the body to the lower limbs. The sacrum is formed by collapse of the intervertebral discs (IVDs) between the five sacral vertebrae during childhood, and their fusion to form a single bone. We show that collapse of the sacral discs in the mouse is associated with the down-regulation of sonic hedgehog (SHH) signaling in the nucleus pulposus (NP) of the disc, and many aspects of this phenotype can be reversed by experimental postnatal activation of HH signaling...
May 21, 2018: Biology Open
https://www.readbyqxmd.com/read/29782610/castration-induces-satellite-cell-activation-that-contributes-to-skeletal-muscle-maintenance
#2
Alanna Klose, Wenxuan Liu, Nicole D Paris, Sophie Forman, John J Krolewski, Kent L Nastiuk, Joe V Chakkalakal
Background: Sarcopenia, the age-related loss of skeletal muscle, is a side effect of androgen deprivation therapy (ADT) for prostate cancer patients. Resident stem cells of skeletal muscle, satellite cells (SCs), are an essential source of progenitors for the growth and regeneration of skeletal muscle. Decreased androgen signaling and deficits in the number and function of SCs are features of aging. Although androgen signaling is known to regulate skeletal muscle, the cellular basis for ADT-induced exacerbation of sarcopenia is unknown...
2018: JCSM rapid communications
https://www.readbyqxmd.com/read/29772900/pyrene-fused-s-indacene
#3
Jason Melidonie, Junzhi Liu, Yubin Fu, Jan J Weigand, Reinhard Berger, Xinliang Feng
One antiaromatic polycyclic hydrocarbon (PH) with and without solubilizing tert-butyl substituents, namely s-indaceno[2,1- a:6,5- a']dipyrene (IDPs), has been synthesized by a four-step protocol. The IDPs represent the longitudinal, peri-extension of the indeno[1,2- b]fluorene skeleton towards a planar 40 π-electron system. Their structures were unambiguously confirmed by X-ray crystallographic analysis. The optoelectronic properties were studied by UV/Vis absorption spectroscopy and cyclic voltammetry. These studies revealed that peri-fusion renders the IDP derivatives with a narrow optical energy gap of 1...
May 18, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29769343/glycoprotein-3-of-porcine-reproductive-and-respiratory-syndrome-virus-exhibits-an-unusual-hairpin-like-membrane-topology
#4
Minze Zhang, Ludwig Krabben, Fangkun Wang, Michael Veit
The glycoprotein GP3 of the Arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) consists of a cleaved signal peptide, a highly glycosylated domain, a short hydrophobic region and an unglycosylated C-terminal domain. GP3 is supposed to form a complex with GP2 and GP4 in virus particles, but secretion of GP3 from cells has also been reported.We analyzed the membrane topology of GP3 from various PRRSV strains. A fraction of the protein is secreted from transfected cells; GP3 from PRRSV-1 strains to a greater extent than GP3 from PRRSV-2 strains...
May 16, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29768105/identification-of-a-recurrent-lmo7-braf-fusion-in-papillary-thyroid-carcinoma
#5
Huiling He, Wei Li, Pearlly Yan, Ralf Bundschuh, Jackson A Killian, Jadwiga Labanowska, Pamela Brock, Rulong Shen, Nyla A Heerema, Albert de la Chapelle
BACKGROUND: The BRAFV600E mutation is the most common driver in papillary thyroid carcinoma (PTC) tumors. In recent years, gene fusions have also been recognized as important drivers of cancer in PTC. Previous studies have suggested that thyroid tumors with fusion genes frequently display an aggressive course. These observations prompted further exploration of gene fusions in PTC tumors. The aim was to search for previously unrecognized gene fusions using thyroid tissue samples from PTC patients...
May 16, 2018: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/29755319/ambra1-mediated-mitophagy-counteracts-oxidative-stress-and-apoptosis-induced-by-neurotoxicity-in-human-neuroblastoma-sh-sy5y-cells
#6
Anthea Di Rita, Pasquale D'Acunzo, Luca Simula, Silvia Campello, Flavie Strappazzon, Francesco Cecconi
Therapeutic strategies are needed to protect dopaminergic neurons in Parkinson's disease (PD) patients. Oxidative stress caused by dopamine may play an important role in PD pathogenesis. Selective autophagy of mitochondria (mitophagy), mainly regulated by PINK1 and PARKIN, plays an important role in the maintenance of cell homeostasis. Mutations in those genes cause accumulation of damaged mitochondria, leading to nigral degeneration and early-onset PD. AMBRA1ActA is a fusion protein specifically expressed at the mitochondria, and whose expression has been shown to induce a powerful mitophagy in mammalian cells...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29751400/2-3-7-8-tetrachlorodibenzo-p-dioxin-induces-alterations-in-myogenic-differentiation-of-c2c12-cells
#7
Heidi Q Xie, Yingjie Xia, Tuan Xu, Yangsheng Chen, Hualing Fu, Yunping Li, Yali Luo, Li Xu, Karl W K Tsim, Bin Zhao
Dioxin-induced toxicities that affect the development of the motor system have been proposed since many years. However, cellular evidence and the molecular basis for the effects are limited. In this study, a cultured mouse myoblast cell line, C2C12, was utilized to examine the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on myogenic differentiation and expression of acetylcholinesterase (AChE), a neuromuscular transmission-related gene. The results showed that TCDD exposure at 10-10  M repressed the myotube formation of C2C12 cells by disturbing the fusion process and suppressing the expression of myosin heavy chain, a myobute structural protein, and not by induction of cytotoxicity...
April 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29749935/improvement-of-reverse-remodeling-using-electrocardiogram-fusion-optimized-intervals-in-cardiac-resynchronization-therapy-a-randomized-study
#8
Emilce Trucco, José María Tolosana, Elena Arbelo, Ada Doltra, María Ángeles Castel, Eva Benito, Roger Borràs, Eduard Guasch, Silvia Vidorreta, Barbara Vidal, Silvia Montserrat, Marta Sitges, Antonio Berruezo, Josep Brugada, Lluís Mont
OBJECTIVES: The aim of this study was to compare patient response to cardiac resynchronization therapy (CRT) using fusion-optimized atrioventricular (AV) and interventricular (VV) intervals versus nominal settings. BACKGROUND: The additional benefit obtained by AV- and VV-interval optimization in patients undergoing CRT remains controversial. Previous studies show short-term benefit in hemodynamic parameters; however, midterm randomized comparison between electrocardiogram optimization and nominal parameters is lacking...
February 2018: JACC. Clinical Electrophysiology
https://www.readbyqxmd.com/read/29744522/-impact-of-thyroid-diseases-on-bone
#9
REVIEW
E Tsourdi, F Lademann, H Siggelkow
Thyroid hormones are key regulators of skeletal development in childhood and bone homeostasis in adulthood, and thyroid diseases have been associated with increased osteoporotic fractures. Hypothyroidism in children leads to an impaired skeletal maturation and mineralization, but an adequate and timely substitution with thyroid hormones stimulates bone growth. Conversely, hyperthyroidism at a young age accelerates skeletal development, but may also cause short stature because of a premature fusion of the growth plates...
May 9, 2018: Der Internist
https://www.readbyqxmd.com/read/29733646/drug-inducible-control-of-lethality-genes-a-low-background-destabilizing-domain-architecture-applied-to-the-gal4-uas-system-in-drosophila
#10
Manjunatha Kogenaru, Mark Isalan
Destabilizing domains (DDs) are genetic tags that conditionally control the level of abundance of proteins-of-interest (POI) with specific stabilizing small-molecule drugs, rapidly and reversibly, in a wide variety of organisms. The amount of the DD-tagged fusion protein directly impacts its molecular function. Hence, it is important that the background levels be tightly regulated in the absence of any drug. This is especially true for classes of proteins that function at extremely low levels, such as lethality genes involved in tissue development and certain transcriptional activator proteins...
May 7, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29731853/targeted-gene-therapy-of-the-hsv-tk-hil-12-fusion-gene-controlled-by-the-hslpi-gene-promoter-of-human-non-small-cell-lung-cancer-in-vitro
#11
Shuhong Hao, Xiaoyuan Du, Yang Song, Ming Ren, Qiwei Yang, Ao Wang, Qingyu Wang, Haiyue Zhao, Zhenwu Du, Guizhen Zhang
The incidence of lung cancer and lung cancer-associated mortality have markedly increased worldwide, and gene-targeted therapy has emerged as a promising treatment strategy. The present study aimed to explore the targeted antitumor effect of the herpes simplex virus-thymidine kinase/human interleukin-12 ( HSV-TK/hIL-12 ) fusion gene regulated by the human secretory leukocyte protease inhibitor ( hSLPI ) promoter of human non-small cell lung cancer (hNSCLC). There were four recombinant eukaryotic expression vectors: pcDNA3...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29721359/acute-cauda-equina-syndrome-following-orthopedic-procedures-as-a-result-of-epidural-anesthesia
#12
Lisa B E Shields, Vasudeva G Iyer, Yi Ping Zhang, Christopher B Shields
Background: Cauda equina syndrome (CES) is a rare complication of spinal or epidural anesthesia. It is attributed to direct mechanical injury to the spinal roots of the cauda equina that may result in saddle anesthesia and paraplegia with bowel and bladder dysfunction. Case Description: The first patient underwent a hip replacement and received 5 mL of 1% lidocaine epidural anesthesia. Postoperatively, when the patient developed an acute CES, the lumbar magnetic resonance imaging (MRI) scan demonstrated clumping/posterior displacement of nerve roots of the cauda equina consistent with adhesive arachnoiditis attributed to the patient's previous L4-L5 lumbar decompression/fusion...
2018: Surgical Neurology International
https://www.readbyqxmd.com/read/29720259/human-serum-and-platelet-lysate-are-appropriate-xeno-free-alternatives-for-clinical-grade-production-of-human-mustem-cell-batches
#13
Charlotte Saury, Aurélie Lardenois, Cindy Schleder, Isabelle Leroux, Blandine Lieubeau, Laurent David, Marine Charrier, Laëtitia Guével, Sabrina Viau, Bruno Delorme, Karl Rouger
BACKGROUND: Canine MuStem cells have demonstrated regenerative efficacy in a dog model of muscular dystrophy, and the recent characterization of human counterparts (hMuStem) has highlighted the therapeutic potential of this muscle-derived stem cell population. To date, these cells have only been generated in research-grade conditions. However, evaluation of the clinical efficacy of any such therapy will require the production of hMuStem cells in compliance with good manufacturing practices (GMPs)...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29713041/rna-sequencing-of-xp11-translocation-associated-cancers-reveals-novel-gene-fusions-and-distinctive-clinicopathologic-correlations
#14
Xiao-Tong Wang, Qiu-Yuan Xia, Sheng-Bing Ye, Xuan Wang, Rui Li, Ru Fang, Shan-Shan Shi, Ru-Song Zhang, Xiao Tan, Jie-Yu Chen, Ke Sun, Xiao-Dong Teng, Heng-Hui Ma, Zhen-Feng Lu, Xiao-Jun Zhou, Qiu Rao
Both Xp11 translocation renal cell carcinomas and the corresponding mesenchymal neoplasms are characterized by a variety of gene fusions involving TFE3. It has been known that tumors with different gene fusions may have different clinicopathologic features; however, further in-depth investigations of subtyping Xp11 translocation-associated cancers are needed in order to explore more meaningful clinicopathologic correlations. A total of 22 unusual cases of Xp11 translocation-associated cancers were selected for the current study; 20 cases were further analyzed by RNA sequencing to explore their TFE3 gene fusion partners...
April 30, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29709714/molecular-analyses-in-the-diagnosis-and-prediction-of-prognosis-in-non-gist-soft-tissue-sarcomas-a-systematic-review-and-meta-analysis
#15
REVIEW
Rita A Kandel, Xiaomei Yao, Brendan C Dickson, Michelle Ghert, Snezana Popovic, Bibianna M Purgina, Shailendra Verma, Joel Werier
BACKGROUND: The molecular pathogenesis of many forms of soft tissue sarcomas (STS) have been rigorously characterized in the medical literature, which may be particularly important for the diagnosis and prediction of prognosis in STS. METHODS: Electronic databases (2005 to October 2016) were searched. Gastrointestinal stromal tumor and pediatric sarcomas were excluded. The eligible individual study's risk of bias and the quality of aggregate evidence were assessed...
May 2018: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/29708737/a-novel-lipidomics-workflow-for-improved-human-plasma-identification-and-quantification-using-rplc-msn-methods-and-isotope-dilution-strategies
#16
Evelyn Rampler, Angela Criscuolo, Martin Zeller, Yasin El Abiead, Harald Schoeny, Gerrit Hermann, Elena Sokol, Ken Cook, David A Peake, Bernard Delanghe, Gunda Koellensperger
Lipid identification and quantification are essential objectives in comprehensive lipidomics studies challenged by the high number of lipids, their chemical diversity and their dynamic range. In this work, we developed a tailored method for profiling and quantification combining (1) isotope dilution, (2) enhanced isomer separation by C30 fused-core reversed-phase material and (3) parallel Orbitrap and ion trap detection by the Orbitrap Fusion Lumos Tribid mass spectrometer. The combination of parallelizable ion analysis without time loss together with different fragmentation techniques (HCD/CID) and an inclusion list led to higher quality in lipid identifications exemplified in human plasma and yeast samples...
April 30, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29707837/self-assembled-breathing-grana-like-cisternae-stacks
#17
Qingchuan Li, Xiaojun Han
Membranes in cells display elaborate, dynamic morphologies intimately tied to defined cellular functions. Cisternae stacks are a common membrane morphology in cells widely found in organelles. However, compared with the well-studied spherical cell membrane mimics, cisternae stacks as organelle membrane mimics are greatly neglected because of the difficulty of fabricating this unique structure. Herein, the grana-like cisternae stacks are assembled via the reorganization of stacked microsized bicelles to mimic grana functions...
April 30, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29705861/the-prognostic-role-of-e2a-pbx1-expression-detected-by-real-time-quantitative-reverse-transcriptase-polymerase-chain-reaction-rq-pcr-in-b-cell-acute-lymphoblastic-leukemia-after-allogeneic-hematopoietic-stem-cell-transplantation
#18
Yan Hong, Xiaosu Zhao, Yazhen Qin, Songhai Zhou, Yingjun Chang, Yu Wang, Xiaohui Zhang, Lanping Xu, Xiaojun Huang
The E2A-PBX1 rearrangement is common in B cell acute lymphoblastic leukemia (B-ALL). However, whether this fusion gene can be used as a reliable marker for minimal residual disease (MRD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains unknown. In this study, clinical data were collected from 28 consecutive B-ALL patients who received allo-HSCT. Their MRD was evaluated by E2A-PBX1 and leukemia-associated immunophenotype (LAIP). The median follow-up was 374 days (55-2342 days)...
April 28, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29703757/telomerase-promoter-mutations-and-copy-number-alterations-in-solitary-fibrous-tumours
#19
Yingbo Lin, Nelly Seger, Panagiotis Tsagkozis, Asle C Hesla, Mehran Ghaderi, Yi Chen, Monika Ehnman, Dudi Warsito, Johan Wejde, Olle Larsson, Felix Haglund
AIMS: Solitary fibrous tumour (SFT) is an infrequently metastasising mesenchymal tumour defined by the NAB2-STAT6 fusion gene. Activating mutations in the telomerase reverse transcriptase ( hTERT ) gene promoter has been reported to associate with adverse patient outcome in SFTs. METHODS: We analysed the hTERT gene for promoter mutations and copy number alterations in 43 primary extrameningeal SFTs (9 malignant and 34 benign tumours according to WHO 2013 criteria), six local recurrences and three metastatic lesions...
April 27, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29700317/pgc1%C3%AE-promotes-cholangiocarcinoma-metastasis-by-upregulating-pdha1-and-mpc1-expression-to-reverse-the-warburg-effect
#20
Dan Li, Chaoqun Wang, Panfei Ma, Qingan Yu, Mingqi Gu, Liqian Dong, Wenjing Jiang, Shangha Pan, Changming Xie, Jihua Han, Yaliang Lan, Jing Sun, Ping Sheng, Kunpeng Liu, Yaohua Wu, Lianxin Liu, Yong Ma, Hongchi Jiang
PGC1α acts as a central regulator of mitochondrial metabolism, whose role in cancer progression has been highlighted but remains largely undefined. Especially, it is completely unknown about the effect of PGC1α on cholangiocarcinoma (CCA). Here we showed that PGC1α overexpression had no impact on CCA growth despite the decreased expression of PGC1α in CCA compared with adjacent normal tissue. Instead, PGC1α overexpression-promoted CCA metastasis both in vitro and in vivo. Mechanistically, for the first time, we illuminated that PGC1α reversed the Warburg effect by upregulating the expression of pyruvate dehydrogenase E1 alpha 1 subunit and mitochondrial pyruvate carrier 1 to increase pyruvate flux into the mitochondria for oxidation, whereas simultaneously promoting mitochondrial biogenesis and fusion to mediate the metabolic switch to oxidative phosphorylation...
April 27, 2018: Cell Death & Disease
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