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Reverse fusion

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https://www.readbyqxmd.com/read/28534479/efficient-protein-production-inspired-by-how-spiders-make-silk
#1
Nina Kronqvist, Médoune Sarr, Anton Lindqvist, Kerstin Nordling, Martins Otikovs, Luca Venturi, Barbara Pioselli, Pasi Purhonen, Michael Landreh, Henrik Biverstål, Zigmantas Toleikis, Lisa Sjöberg, Carol V Robinson, Nicola Pelizzi, Hans Jörnvall, Hans Hebert, Kristaps Jaudzems, Tore Curstedt, Anna Rising, Jan Johansson
Membrane proteins are targets of most available pharmaceuticals, but they are difficult to produce recombinantly, like many other aggregation-prone proteins. Spiders can produce silk proteins at huge concentrations by sequestering their aggregation-prone regions in micellar structures, where the very soluble N-terminal domain (NT) forms the shell. We hypothesize that fusion to NT could similarly solubilize non-spidroin proteins, and design a charge-reversed mutant (NT*) that is pH insensitive, stabilized and hypersoluble compared to wild-type NT...
May 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28527402/a-rare-e13a3-b2a3-bcr-abl1-fusion-transcript-with-normal-karyotype-in-chronic-myeloid-leukemia-the-challenges-in-diagnosis-and-monitoring-minimal-residual-disease-mrd
#2
M-H Duan, H Li, H Cai
Patients with chronic myeloid leukemia (CML) have a t (9;22)(q34;q11.2) or variant translocation that results in a BCR-ABL1 fusion gene. For many years, conventional karyotyping has been used as the standard diagnostic tool for t (9;22) (q34;q11.2). However, it has several limitations that may lead to failure for detecting BCR-ABL1 gene rearrangements in around 5% of all CML patients. Although reverse transcription polymerase chain reaction (RT-PCR) has evolved as a sensitive method for detecting BCR-ABL1 translocation, this method fail to detect certain BCR-ABL1 fusion transcript type, such as e13a3 (also known as b2a3), as a result of many commercially available and laboratory-developed primer sets...
May 12, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28507416/pathotypic-characterization-of-newcastle-disease-virus-isolated-from-vaccinated-chicken-in-west-java-indonesia
#3
Dwi Desmiyeni Putri, Ekowati Handharyani, Retno Damajanti Soejoedono, Agus Setiyono, Ni Luh Putu Ika Mayasari, Okti Nadia Poetri
AIM: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers. MATERIALS AND METHODS: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene...
April 2017: Veterinary World
https://www.readbyqxmd.com/read/28504877/illuminating-superoxide-anion-and-ph-enhancements-in-apoptosis-of-breast-cancer-cells-induced-by-mitochondrial-hyperfusion-using-a-new-two-photon-fluorescence-probe
#4
Wen Zhang, Xin Wang, Ping Li, Haibin Xiao, Wei Zhang, Hui Wang, Bo Tang
Mitochondrial morphology regulated by fusion and fission processes determines mitochondrial function and cell fate. Some studies showed hyperfused mitochondria could induce apoptosis in cancer cells, but the relevant molecular mechanisms remain elusive. Superoxide (O2•-) and pH play vital roles in mitochondrial dysfunction and apoptosis. Therefore, it is worthwhile to explore if there is an intimate relationship between mitochondrial hyperfusion and simultaneous changes in O2•- and pH levels, which will be helpful to uncover relevant detailed mechanism...
May 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28503736/hydrogen-sulfide-pretreatment-improves-mitochondrial-function-in-myocardial-hypertrophy-via-a-sirt3-dependent-manner
#5
Guoliang Meng, Jieqiong Liu, Shangmin Liu, Qiuyi Song, Lulu Liu, Liping Xie, Yi Han, Yong Ji
BACKGROUND AND PURPOSE Hydrogen sulfide (H2 S) is a gaseous signal molecule with anti-oxidative ability. Sirtuin 3 (SIRT3) is closely associated with mitochondrial function and oxidative stress. The study was to investigate whether and how H2 S improved myocardial hypertrophy via a SIRT3-dependent manner. EXPERIMENTAL APPROACH Neonatal rat cardiomyocytes were pre-treated with NaHS (50 μM) for 4 h followed by angiotensin II (Ang II, 100 nM) for 24 h. SIRT3 was silenced with siRNA technology. SIRT 3 promoter activity and expression, cell surface, hypertrophic gene mRNA expression, mitochondrial oxygen consumption rate and membrane potential were measured...
May 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28501614/shaping-mitochondrial-dynamics-the-role-of-camp-signalling
#6
Giulietta Di Benedetto, Andrea Gerbino, Konstantinos Lefkimmiatis
In recent years, our idea of mitochondria evolved from "mere" energy and metabolite producers to key regulators of many cellular functions. In order to preserve and protect their functional status, these organelles engage a number of dynamic processes that allow them to decrease accumulated burden and maintain their homeostasis. Indeed, mitochondria can unite (fusion), divide (fission), position themselves strategically in the cell (motility/trafficking) and if irreversibly damaged or dysfunctional eliminated (mitophagy)...
May 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28498445/evaluation-of-expression-of-cancer-stem-cell-markers-and-fusion-gene-in-synovial-sarcoma-insights-into-histogenesis-and-pathogenesis
#7
Yang Zhou, Dongdong Chen, Yan Qi, Ruixue Liu, Shugang Li, Hong Zou, Jiaojiao Lan, Xinxin Ju, Jinfang Jiang, Weihua Liang, Yaoyuan Shen, Lijuan Pang, Feng Li
Synovial sarcoma (SS) is an aggressive soft tissue tumor, with uncertain histological and cellular origin. SYT-SSX is considered to be responsible for sarcoma initiation and progression. The histogenesis and pathogenesis of this tumor are poorly understood, and prognosis of patients of SS is unsatisfactory. Recent studies have shown an association of cancer stem cells with the initiation and development of tumors. We explored immunohistochemical expression level of stem cell associated markers to determine the possible histogenesis and pathogenesis of SS...
May 2, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28496950/development-of-flow-cytometry-fluorescent-in-situ-hybridization-flow-fish-method-for-detection-of-pml-rara-chromosomal-translocation-in-acute-promyelocytic-leukemia-cell-line
#8
Fatemeh Zahedipour, Reza Ranjbaran, Abbas Behzad Behbahani, Khalil Tavakol Afshari, Mohammad Ali Okhovat, Gholamhossein Tamadon, Sedigheh Sharifzadeh
BACKGROUND: Acute Promyelocytic Leukemia (APL) is a subclass of acute myeloid leukemia. The chromosomal aberration in 95% of APL cases is t(15; 17) (q22; q21), which prevents cell differentiation. Characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. The goal of this study was to develop a new and powerful Flow- FISH technique to detect the long isoform (L) of PML-RARa fusion transcript in NB4 cell line. METHODS: To achieve the best condition for fixation, two different fixatives including 2% paraformaldehyde and 75% ethanol were used...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28494796/a-malignant-solitary-fibrous-tumour-arising-from-the-first-lumbar-vertebra-and-mimicking-an-osteosarcoma-a-case-report
#9
Naoki Oike, Hiroyuki Kawashima, Akira Ogose, Tetsuo Hotta, Toru Hirano, Takashi Ariizumi, Tetsuro Yamagishi, Hajime Umezu, Shoichi Inagawa, Naoto Endo
BACKGROUND: A solitary fibrous tumour (SFT) is an unusual neoplasm typically found in soft tissues. Although SFTs can arise in the bones, they very rarely arise in the vertebral arch. Here, we describe a case of a SFT that arose in the vertebral arch of the first lumbar (L1) spinal vertebrae and mimicked osteosarcoma. CASE PRESENTATION: A 49-year-old woman presented with a 2-month history of lower back pain and a lumbar region mass. Magnetic resonance imaging demonstrated a heterogeneously enhanced mass in the L1 vertebral arch...
May 11, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28494506/minimal-residual-disease-eradication-with-epigenetic-therapy-in-core-binding-factor-acute-myeloid-leukemia
#10
Brittany Knick Ragon, Naval Daver, Guillermo Garcia-Manero, Farhad Ravandi, Jorge Cortes, Tapan Kadia, Betul Oran, Maro Ohanian, Alessandra Ferrajoli, Naveen Pemmaraju, Hagop M Kantarjian, Gautam Borthakur
Recurrent translocations, t(8;21) or inv(16), in core binding factor acute myeloid leukemia (CBF-AML) are amenable to monitoring for minimal residual disease (MRD) with reverse transcriptase polymerase chain reaction (RTPCR). Despite a favorable prognosis, disease relapse remains the single cause of treatment failure in CBF-AML. Fusion products of these translocations recruit epigenetic silencing complexes resulting in hematopoietic maturation arrest. We hypothesized that maintenance therapy with hypomethylating agents (HMA), including decitabine (DAC) and azacitidine (AZA) after induction/consolidation, can be used for MRD elimination to ultimately prolong relapse free survival...
May 11, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28494151/design-of-hiv-co-receptor-derived-peptides-that-inhibit-viral-entry-at-submicromolar-concentrations
#11
Kostyantyn D Bobyk, Sivakoteswara R Mandadapu, Katheryn Lohith, Christina Guzzo, Abhishek Bhargava, Paolo Lusso, Carole A Bewley
HIV/AIDS continues to pose an enormous burden on global health. Current HIV therapeutics include inhibitors that target the enzymes HIV protease, reverse transcriptase and integrase, along with viral entry inhibitors that block the initial steps of HIV infection by preventing membrane fusion or virus-coreceptor interactions. With regard to the latter, peptides derived from the HIV coreceptor CCR5 were previously shown to modestly inhibit entry of CCR5-tropic HIV strains, with a peptide containing residues 178-191 of the second extracellular loop (peptide 2C) showing the strongest inhibition...
May 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28490595/sensing-of-hiv-1-entry-triggers-a-type-i-interferon-response-in-human-primary-macrophages
#12
Jérémie Decalf, Marion Desdouits, Vasco Rodrigues, François-Xavier Gobert, Matteo Gentili, Santy Marques-Ladeira, Célia Chamontin, Marylène Mougel, Bruna Cunha de Alencar, Philippe Benaroch
Along with CD4+ T lymphocytes, macrophages are a major cellular source of HIV-1 replication, and a potential viral reservoir. Following entry and reverse transcription in macrophages, cloaking of the viral cDNA by the HIV-1 capsid limits its cytosolic detection, enabling efficient replication. However, whether incoming HIV-1 particles are sensed by macrophages prior to reverse-transcription, remains unclear. Here, we show that HIV-1 triggers a broad expression of interferon-stimulated genes (ISG) in monocyte-derived macrophages within a few hours after infection...
May 10, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28489900/scanning-double-sided-documents-without-incurring-show-through-by-learning-to-fuse-two-complementary-images-using-multilayer-perceptron
#13
Yuzhong Chen
This paper presents a novel method for scanning duplex-printed documents without incurring the unwanted show-through artifact. The proposed method achieves the goal of eliminating the leaked-out reverse-side content by fusing a white backed scan image with a black backed scan image of the document. The fusion is accomplished using a multilayer perceptron having learned a fusion mapping from manually corrected document images. The main novel contributions of this work include (1) being the first to propose to accomplish the goal of show through free scanning by fusing a white backed scan image with a black backed scan image of the document; (2) proposing a learning approach using a multilayer perceptron to learn the fusion mapping from manually corrected scan images; and (3) proposing to use the pixel value histogram of reverse-side-printed area as well as the pixel value histogram of duplex-printed area to quantitatively indicate show through severity to facilitate objective comparison of the methods in consideration...
2017: PloS One
https://www.readbyqxmd.com/read/28489068/tnf%C3%AE-drives-mitochondrial-stress-in-pomc-neurons-in-obesity
#14
Chun-Xia Yi, Marc Walter, Yuanqing Gao, Soledad Pitra, Beata Legutko, Stefanie Kälin, Clarita Layritz, Cristina García-Cáceres, Maximilian Bielohuby, Martin Bidlingmaier, Stephen C Woods, Alexander Ghanem, Karl-Klaus Conzelmann, Javier E Stern, Martin Jastroch, Matthias H Tschöp
Consuming a calorically dense diet stimulates microglial reactivity in the mediobasal hypothalamus (MBH) in association with decreased number of appetite-curbing pro-opiomelanocortin (POMC) neurons; whether the reduction in POMC neuronal function is secondary to the microglial activation is unclear. Here we show that in hypercaloric diet-induced obese mice, persistently activated microglia in the MBH hypersecrete TNFα that in turn stimulate mitochondrial ATP production in POMC neurons, promoting mitochondrial fusion in their neurites, and increasing POMC neuronal firing rates and excitability...
May 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28486561/myosin-phosphatase-and-rhoa-activated-kinase-modulate-neurotransmitter-release-by-regulating-snap-25-of-snare-complex
#15
Dániel Horváth, István Tamás, Adrienn Sipos, Zsuzsanna Darula, Bálint Bécsi, Dénes Nagy, Judit Iván, Ferenc Erdődi, Beáta Lontay
Reversible phosphorylation of neuronal proteins plays an important role in the regulation of neurotransmitter release. Myosin phosphatase holoenzyme (MP) consists of a protein phosphatase-1 (PP1) catalytic subunit (PP1c) and a regulatory subunit, termed myosin phosphatase targeting subunit (MYPT1). The primary function of MP is to regulate the phosphorylation level of contractile proteins; however, recent studies have shown that MP is localized to neurons, and is also involved in the mediation of neuronal processes...
2017: PloS One
https://www.readbyqxmd.com/read/28485950/molecular-scale-design-of-hydrocarbon-surfactant-self-assembly-in-supercritical-co2
#16
Muhan Wang, Timing Fang, Pan Wang, Youguo Yan, Jun Zhang, Bing Liu, Xiaoli Sun
Forming wormlike reverse micelles (RMs) by hydrocarbon surfactant self-assembly is an economic and environmental strategy to improve the physicochemical properties of supercritical carbon dioxide (scCO2), but it remains challenging. Introducing cosurfactant in hydrocarbon surfactant self-assembly system is a potential method to generate wormlike RMs. Here, adopting molecular dynamics simulations, we performed hydrocarbon surfactant (TC14) self-assembly with introducing cosurfactants (C8Benz). It is found that adding the C8Benz molecules will induce the spherical RMs to a short rodlike form...
May 15, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28485547/pseudoginsenoside-f11-attenuates-cerebral-ischemic-injury-by%C3%A2-alleviating-autophagic-lysosomal-defects
#17
Yue-Yang Liu, Tian-Yu Zhang, Xue Xue, Dong-Mei Liu, Hao-Tian Zhang, Lin-Lin Yuan, Ying-Lu Liu, Han-Lin Yang, Shi-Bo Sun, Cheng Zhang, He-Song Xu, Chun-Fu Wu, Jing-Yu Yang
AIMS: Pseudoginsenoside-F11 (PF11), an ocotillol-type ginsenoside, has been reported to exert wide-ranging neuroprotective properties. The aim of this study was to investigate the effect and potential mechanisms of PF11 on the autophagic/lysosomal pathway following ischemic stroke. METHODS: Male Sprague-Dawley rats underwent permanent middle cerebral artery occlusion (pMCAO). Cerebral ischemia outcome, TUNEL staining, Fluoro-Jade B staining were carried out 24 hours poststroke...
May 9, 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/28475434/enhancing-acute-myeloid-leukemia-therapy-monitoring-response-using-residual-disease-testing-as-a-guide-to-therapeutic-decision-making
#18
Benjamin Tomlinson, Hillard M Lazarus
Current standards for monitoring the response of acute myeloid leukemia (AML) are based on morphologic assessments of the bone marrow and recovery of peripheral blood counts. A growing experience is being developed to enhance the detection of small amounts of AML, or minimal residual disease (MRD). Areas covered: Available techniques include multi-color flow cytometry (MFC) of leukemia associated immunophenotypes (LAIP), quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) for detecting fusion and mutated genes (RUNX1-RUNX1T1, CBFB-MYH11, and NPM1), overexpression of genes such as WT1, and next generation sequencing (NGS) for MRD...
May 12, 2017: Expert Review of Hematology
https://www.readbyqxmd.com/read/28470873/bmp9-induces-osteogenesis-and-adipogenesis-in-the-immortalized-human-cranial-suture-progenitors-from-the-patent-sutures-of-craniosynostosis-patients
#19
Dongzhe Song, Fugui Zhang, Russell R Reid, Jixing Ye, Qiang Wei, Junyi Liao, Yulong Zou, Jiaming Fan, Chao Ma, Xue Hu, Xiangyang Qu, Liqun Chen, Li Li, Yichun Yu, Xinyi Yu, Zhicai Zhang, Chen Zhao, Zongyue Zeng, Ruyi Zhang, Shujuan Yan, Tingting Wu, Xingye Wu, Yi Shu, Jiayan Lei, Yasha Li, Wenwen Zhang, Jia Wang, Michael J Lee, Jennifer Moriatis Wolf, Dingming Huang, Tong-Chuan He
The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and mesenchymal stem cells (MSCs) from bone marrow and suture mesenchyme. The fusion of cranial sutures is a highly coordinated and tightly regulated process during development. Craniosynostosis is a congenital malformation caused by premature fusion of cranial sutures. While the progenitor cells derived from the cranial suture complex should prove valuable for studying the molecular mechanisms underlying suture development and pathogenic premature suture fusion, primary human cranial suture progenitors (SuPs) have limited life span and gradually lose osteoblastic ability over passages...
May 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28458917/adaptation-of-avian-influenza-virus-to-a-swine-host
#20
Vincent Bourret, Jon Lyall, Simon D W Frost, Angélique Teillaud, Catherine A Smith, Sarah Leclaire, JinQi Fu, Sylvain Gandon, Jean-Luc Guérin, Laurence S Tiley
The emergence of pathogenic RNA viruses into new hosts can have dramatic consequences for both livestock and public health. Here we characterize the viral genetic changes that were observed in a previous study which experimentally adapted a field isolate of duck influenza virus to swine respiratory cells. Both pre-existing and de novo mutations were selected during this adaptation. We compare the in vitro growth dynamics of the adapted virus with those of the original strain as well as all possible reassortants using reverse genetics...
January 2017: Virus Evolution
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