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https://www.readbyqxmd.com/read/29464200/polyomavirus-bk-nephropathy-associated-transcriptomic-signatures-a-critical-reevaluation
#1
Ling Pan, Zili Lyu, Benjamin Adam, Gang Zeng, Zijie Wang, Yuchen Huang, Zahidur Abedin, Parmjeet Randhawa
Background: Recent work using DNA microarrays has suggested that genes related to DNA replication, RNA polymerase assembly, and pathogen recognition receptors can serve as surrogate tissue biomarkers for polyomavirus BK nephropathy (BKPyVN). Methods: We have examined this premise by looking for differential regulation of these genes using a different technology platform (RNA-seq) and an independent set 25 biopsies covering a wide spectrum of diagnoses. Results: RNA-seq could discriminate T cell-mediated rejection from other common lesions seen in formalin fixed biopsy material...
February 2018: Transplantation Direct
https://www.readbyqxmd.com/read/29462791/the-cellular-chloride-channels-clic1-and-clic4-contribute-to-virus-mediated-cell-motility
#2
Gabrielė Stakaitytė, Nnenna Nwogu, Jonathan D Lippiat, G Eric Blair, Krzysztof Poterlowicz, James R Boyne, Andrew Macdonald, Jamel Mankouri, Adrian Whitehouse
Ion channels regulate many aspects of cell physiology, including cell proliferation, motility, and migration, and aberrant expression and activity of ion channels is associated with various stages of tumor development, with K+ and Cl- channels now being considered the most active during tumorigenesis. Accordingly, emerging in vitro and preclinical studies have revealed that pharmacological manipulation of ion channel activity offers protection against several cancers. Merkel cell polyomavirus (MCPyV) is a major cause of Merkel cell carcinoma (MCC), primarily due to the expression of two early regulatory proteins termed small and large tumour antigens (ST and LT, respectively)...
February 8, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29460539/human-polyomavirus-in-tonsillar-microbiota-of-an-afghan-population-group
#3
F Ruggiero, D Carbone, R Mugavero, A Palmieri, D Lauritano, L Baggi, M Nardone, M Martinelli, F Carinci
Some studies have evidenced the role of human polyomaviruses in head and neck squamous cell carcinoma. BK, JC and SV40 human polyoma viruses are widely recognized as etiological agents associated with malignancies. The aim of this study was to analyse the prevalence of BK, IC and SV40 in tonsillar microbiota in a group of Afghan volunteers. A sample of the tonsillar microbiota was taken from a single site using a sterile oral swab paper stick. A fixed volume of purified DNA from each sample was tested by quantitative real-time polymerase chain reactions to evaluate the number of human cells and the number of viral genomes in each sample...
January 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29458134/antiviral-activity-of-pyrrole-imidazole-polyamides-against-sv40-and-bk-polyomaviruses
#4
Terri G Edwards, Chris Fisher
The ability of antiviral polyamides (AVP) to act upon polyomaviruses (PyV) was evaluated. Initial studies found that a single treatment of AVP protected SV40-infected BSC-1 cells from cytopathic effect (CPE) for as long as 11 days p.i.. AVP substantially suppressed SV40 genome copy numbers over the duration of the experiment. Immunofluorescence analysis of ataxia-telangiectasia mutated (ATM) activation and large T antigen (LTag) expression clearly demonstrated that AVP treatment at day 1 p.i. delayed the onset of productive SV40 replication by approximately 3 days, and substantially limited the infection relative to vehicle-treated controls...
February 16, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29456214/outcome-of-renal-transplant-recipients-with-cytomegalovirus-and-bk-polyomavirus-co-infection-nephropathy
#5
Anupma Kaul, Shashi Kumar, Dharmendra Bhaduaria, Vinita Agrawal, R K Sharma, Narayan Prasad, Amit Gupta, Rishi Kumar
Reactivation of cytomegalovirus (CMV) and BK polyomavirus (BKV) can result in virus-associated tubulointerstitial nephritis in renal allografts. All those renal biopsies reported as viral cytopathic were isolated and examined by two independent renal histopathologists from our institute and classified as CMV, BKV, and CMV-BKV coinfection-associated viral cytopathic changes with confirmation through polymerase chain reaction technology in either serum or urine or both. All twenty patients were categorized as 10 in CMV, four in BKV, and six were in CMV-BKV coinfection...
January 2018: Saudi Journal of Kidney Diseases and Transplantation
https://www.readbyqxmd.com/read/29451655/human-polyomavirus-infection-cytological-and-molecular-diagnosis
#6
Patricia Gioia de Assis, Maria da Glória da Costa Carvalho
Few studies directly compare urinary cytology with molecular methods for detecting BK and JC polyomaviruses. Reactivation of BKV infection is the main risk factor for the development of nephropathy in immunocompromised individuals. The limitation of the cytological method can be attributed to the stage where the infected cell does not have specific and sufficient morphological characteristics for a conclusive diagnosis and can be easily interpreted as degenerative alteration. Moreover, morphologically, it is not possible to differentiate the two types of viruses...
November 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/29447282/addition-of-m6a-to-sv40-late-mrnas-enhances-viral-structural-gene-expression-and-replication
#7
Kevin Tsai, David G Courtney, Bryan R Cullen
Polyomaviruses are a family of small DNA tumor viruses that includes several pathogenic human members, including Merkel cell polyomavirus, BK virus and JC virus. As is characteristic of DNA tumor viruses, gene expression in polyomaviruses is temporally regulated into an early phase, consisting of the viral regulatory proteins, and a late phase, consisting of the viral structural proteins. Previously, the late transcripts expressed by the prototypic polyomavirus simian virus 40 (SV40) were reported to contain several adenosines bearing methyl groups at the N6 position (m6A), although the precise location of these m6A residues, and their phenotypic effects, have not been investigated...
February 15, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29445241/is-early-life-exposure-to-polyomaviruses-and-herpesviruses-associated-with-obesity-indices-and-metabolic-traits-in-childhood
#8
Marianna Karachaliou, Silvia de Sanjose, Tim Waterboer, Theano Roumeliotaki, Maria Vassilaki, Katerina Sarri, Vasiliki Leventakou, Marina Vafeiadi, Georgia Chalkiadaki, Eftichia Stiakaki, Angelika Michel, Michael Pawlita, Manolis Kogevinas, Leda Chatzi
BACKGROUND: Evidence for an infectious origin of obesity is emerging. We explored whether common viruses were associated with obesity and metabolic traits. METHODS: We used cross-sectional (n = 674) and prospective (n = 440) data from children participating at the 4 and 6 years of age follow-up in the Rhea birth cohort. Presence of IgG antibodies to ten polyomaviruses (BKPyV, JCPyV, KIPyV, WUPyV, HPyV6, HPyV7, TSPyV, MCPyV, HPyV9, and HPyV10) and four herpesviruses (EBV, CMV, HSV-1, and HSV-2) were measured at age 4...
January 30, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29435709/the-prevalence-and-isolated-subtypes-of-bk-polyomavirus-reactivation-among-patients-infected-with-human-immunodeficiency-virus-1-in-southeastern-china
#9
Caiqin Hu, Ying Huang, Juwei Su, Mengyan Wang, Qihui Zhou, Biao Zhu
BK polyomavirus (BKPyV) is an opportunistic infectious pathogen that is associated with hemorrhagic cystitis and nephropathy, mainly in transplant recipients and human immunodeficiency virus 1 (HIV-1) infected patients. However, molecular characterization studies of BKPyV in China are rare. This study was designed to elucidate the prevalence and to determine the main subtypes of BKPyV among HIV-1-infected patients in southeastern China. In addition, the increased incidences for BKPyV reactivation were analyzed...
February 12, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29432481/viral-microrna-effects-on-persistent-infection-of-human-lymphoid-cells-by-polyomavirus-sv40
#10
Adrienne L McNees, Lindsay J Harrigal, Aoife Kelly, Charles G Minard, Connie Wong, Janet S Butel
BACKGROUND: Polyomaviruses, including simian virus 40 (SV40), display evidence of lymphotropic properties. This study analyzed the nature of SV40-human lymphocyte interactions in established cell lines and in primary lymphocytes. The effects of viral microRNA and the structure of the viral regulatory region on SV40 persistence were examined. RESULTS: SV40 DNA was maintained in infected B cell and myeloid cell lines during cell growth for at least 28 days. Limiting dilution analysis showed that low amounts of SV40 DNA (~2 copies per cell) were retained over time...
2018: PloS One
https://www.readbyqxmd.com/read/29430700/a-monoclonal-antibody-to-sv40-large-t-antigen-pab416-does-not-label-merkel-cell-carcinoma
#11
Daniel J Pelletier, Thomas W Czeczok, Andrew M Bellizzi
AIMS: Merkel cell carcinoma represents poorly differentiated neuroendocrine carcinoma of cutaneous origin. In most studies the vast majority of Merkel cell carcinomas are Merkel cell polyomavirus (MCPyV)-associated. SV40 polyomavirus immunohistochemistry is typically used in the diagnosis other polyomavirus-associated diseases including tubulointerstitial nephritis and progressive multifocal leukoencephalopathy, given cross-reactivity with BK and JC polyomaviruses. MCPyV-specific immunohistochemistry is commercially available, but if SV40 immunohistochemistry also cross-reacted with MCPyV that would be advantageous from a resource-utilization perspective...
February 11, 2018: Histopathology
https://www.readbyqxmd.com/read/29427804/escmid-study-group-for-infections-in-compromised-hosts-esgich-consensus-document-on-the-safety-of-targeted-and-biological-therapies-an-infectious-diseases-perspective-immune-checkpoint-inhibitors-cell-adhesion-inhibitors-sphingosine-1-phosphate-receptor-modulators
#12
REVIEW
Gil Redelman-Sidi, Olivier Michielin, Carlos Cervera, Camillo Ribi, José María Aguado, Mario Fernández-Ruiz, Oriol Manuel
BACKGROUND: The present review is part of the ESCMID Study Group for Infections in Compromised Hosts (ESGICH) Consensus Document on the safety of targeted and biological therapies. AIMS: To review, from an Infectious Diseases perspective, the safety profile of immune checkpoint inhibitors, LFA-3-targeted agents, cell adhesion inhibitors, sphingosine-1-phosphate receptor modulators and proteasome inhibitors and to suggest preventive recommendations. SOURCES: Computer-based MEDLINE searches with MeSH terms pertaining to each agent or therapeutic family...
February 7, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29420808/jc-polyoma-viruria-associates-with-protection-from-chronic-kidney-disease-independently-from-apolipoprotein-l1-genotype-in-african-americans
#13
Barry I Freedman, Amy L Kistler, Peter Skewes-Cox, Don Ganem, Mitzie Spainhour, Jolyn Turner, Jasmin Divers, Carl D Langefeld, Mariana Murea, Pamela J Hicks, Ashok K Hemal, James A Snipes, Lihong Zhao, Johanna R Abend, Douglas S Lyles, Lijun Ma, Karl L Skorecki
Background: Viral infections can trigger chronic kidney disease (CKD) and the urine virome may inform risk. The Natural History of APOL1-Associated Nephropathy Study (NHAANS) reported that urine JC polyomavirus (JCPyV) associated with a lower risk of APOL1-associated nephropathy in African Americans. Herein, association was assessed between urine JCPyV with CKD in African Americans independent from the APOL1 genotype. Methods: Quantitative polymerase chain reaction was performed for urinary detection of JCPyV and BK polyoma virus (BKPyV) in 200 newly recruited nondiabetic African Americans...
February 6, 2018: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29414185/prevalence-and-stability-of-antibodies-to-thirteen-polyomaviruses-and-association-with-cutaneous-squamous-cell-carcinoma-a-population-based-study
#14
Annika Antonsson, Rachel E Neale, Peter O'Rourke, Leesa Wockner, Angelika Michel, Michael Pawlita, Tim Waterboer, Adèle C Green
BACKGROUND: Several new members of the human polyomavirus (HPyV) family that infect human skin and are potentially oncogenic have been identified in the last decade. OBJECTIVES: To investigate prospectively the seroprevalence and stability of 13 PyVs, and possible associations with different risk factors and cutaneous squamous cell carcinoma (cSCC). STUDY DESIGN: In this Australian population-based longitudinal study sera were collected at baseline in 1992 or during the next 4 years from 688 people...
January 30, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29409030/genetic-variability-of-the-noncoding-control-region-of-cutaneous-merkel-cell-polyomavirus-identification-of-geographically-related-genotypes
#15
Yumiko Hashida, Tomonori Higuchi, Kiyohiko Matsui, Yuka Shibata, Kimiko Nakajima, Shigetoshi Sano, Masanori Daibata
Background: Merkel cell polyomavirus (MCPyV) is a ubiquitous cutaneous virus that causes Merkel cell carcinoma (MCC), which develops preferentially in white European/North American populations. However, the genomic variations of MCPyV among ethnic groups have not been well delineated, and even less is known regarding alterations in the noncoding control region (NCCR) in the general population. Methods: MCPyV strains recovered from skin swabs of 250 healthy participants with distinct ethnicity/geographic origins were subjected to sequencing analysis of the NCCR...
February 2, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29402297/cos-7-based-model-methodological-approach-to-study-john-cunningham-virus-replication-cycle
#16
C Prezioso, D Scribano, D M Rodio, C Ambrosi, M Trancassini, A T Palamara, V Pietropaolo
John Cunningham virus (JCV) is a human neurotropic polyomavirus whose replication in the Central Nervous System (SNC) induces the fatal demyelinating disease, progressive multifocal leukoencephalopathy (PML). JCV propagation and PML investigation have been severely hampered by the lack of an animal model and cell culture systems to propagate JCV have been very limited in their availability and robustness. We previously confirmed that JCV CY strain efficiently replicated in COS-7 cells as demonstrated by the progressive increase of viral load by quantitative PCR (Q-PCR) during the time of transfection and that archetypal regulatory structure was maintained, although two characteristic point mutations were detected during the viral cycle...
February 5, 2018: Virology Journal
https://www.readbyqxmd.com/read/29396437/gene-therapy-for-human-glioblastoma-using-neurotropic-jc-virus-like-particles-as-a-gene-delivery-vector
#17
Chun-Nun Chao, Yu-Hsuan Yang, Mu-Sheng Wu, Ming-Chieh Chou, Chiung-Yao Fang, Mien-Chun Lin, Chien-Kuo Tai, Cheng-Huang Shen, Pei-Lain Chen, Deching Chang, Meilin Wang
Glioblastoma multiforme (GBM), the most common malignant brain tumor, has a short period of survival even with recent multimodality treatment. The neurotropic JC polyomavirus (JCPyV) infects glial cells and oligodendrocytes and causes fatal progressive multifocal leukoencephalopathy in patients with AIDS. In this study, a possible gene therapy strategy for GBM using JCPyV virus-like particles (VLPs) as a gene delivery vector was investigated. We found that JCPyV VLPs were able to deliver the GFP reporter gene into tumor cells (U87-MG) for expression...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29389139/inhibitor-gcpii-interaction-selective-and-robust-system-for-targeting-cancer-cells-with-structurally-diverse-nanoparticles
#18
Jitka Neburkova, Frantisek Sedlak, Jirina Zackova Suchanova, Libor Kostka, Pavel Sacha, Vladimír Šubr, Tomáš Etrych, Petr Simon, Jitka Barinkova, Robin Krystufek, Hana Spanielova, Jitka Forstova, Jan Konvalinka, Petr Cigler
Glutamate carboxypeptidase II (GCPII) is a membrane protease overexpressed by prostate cancer cells and detected in the neovasculature of most solid tumors. Targeting GCPII with inhibitor-bearing nanoparticles can enable recognition, imaging, and delivery of treatments to cancer cells. Compared to methods based on antibodies and other large biomolecules, inhibitor-mediated targeting benefits from the low molecular weight of the inhibitor molecules, which are typically stable, easy-to-handle, and able to bind the enzyme with very high affinity...
February 1, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29381009/mw-polyomavirus-in-diarrheal-italian-infants
#19
Massimiliano Bergallo, Ilaria Galliano, Paola Montanari, Marco Rassu, Valentina Daprà, Chiara Merlino, Pier-Angelo Tovo
BACKGROUND: MXPyV, like MWPyV, was identified in stool samples from children suffering diarrhea in Mexico. In this study, we used a home-made real time PCR to investigate the presence of this novel viruses in stool specimen collected from under-Five- Year-Old Children with gastroenteritis. METHODS: A total of 192 fecal specimens previously screened for RV, ADV, NoV, HPeV and SaV, were tested for MWPyV with Taqman real time PCR. RESULTS: The most commonly detected virus was NoV GII (33...
January 29, 2018: Minerva Pediatrica
https://www.readbyqxmd.com/read/29374036/the-fate-of-urinary-tract-virus-bk-human-polyomavirus-in-source-separated-urine
#20
Heather E Goetsch, Linbo Zhao, Mariah Gnegy, Michael J Imperiale, Nancy G Love, Krista R Wigginton
Human polyomaviruses are emerging pathogens that infect a large percentage of the human population and are excreted in urine. Consequently, urine that is collected for fertilizer production often has high concentrations of polyomavirus genes. We studied the fate of infectious double-stranded DNA (dsDNA) BK human polyomavirus (BKPyV) in hydrolyzed source-separated urine with infectivity assays and qPCR. Although BKPyV genomes persisted in the hydrolyzed urine for long periods of time (T90 >3 weeks), the viruses were rapidly inactivated (T90 = 1...
January 26, 2018: Applied and Environmental Microbiology
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