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Lactate AND Cancer

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https://www.readbyqxmd.com/read/29445172/rational-cell-culture-optimization-enhances-experimental-reproducibility-in-cancer-cells
#1
Marina Wright Muelas, Fernando Ortega, Rainer Breitling, Claus Bendtsen, Hans V Westerhoff
Optimization of experimental conditions is critical in ensuring robust experimental reproducibility. Through detailed metabolomic analysis we found that cell culture conditions significantly impacted on glutaminase (GLS1) sensitivity resulting in variable sensitivity and irreproducibility in data. Baseline metabolite profiling highlighted that untreated cells underwent significant changes in metabolic status. Both the extracellular levels of glutamine and lactate and the intracellular levels of multiple metabolites changed drastically during the assay...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29444941/lactic-acid-downregulates-viral-microrna-to-promote-epstein-barr-virus-immortalized-b-lymphoblastic-cell-adhesion-and-growth
#2
Xiaohui Mo, Fang Wei, Yin Tong, Ling Ding, Qing Zhu, Shujuan Du, Fei Tan, Caixia Zhu, Yuyan Wang, Qian Yu, Yeqiang Liu, Erle S Robertson, Zhenghong Yuan, Qiliang Cai
High plasma lactate is associated with poor prognosis of many malignancies, but its role in virally mediated cancer progression and underlying molecular mechanisms are unclear. Epstein-Barr virus (EBV), the first human oncogenic virus, causes several cancers, including B cell lymphoma. Here, we report that lactate dehydrogenase (LDH-A) expression and lactate production are elevated in EBV-immortalized B lymphoblastic cells, and lactic acid (LA, acidic lactate) at low concentration triggers EBV-infected B cell adhesion, morphological changes, and proliferation in vitro and in vivo Moreover, LA-induced responses of EBV-infected B cells uniquely occurs in viral latency type III and it is dramatically associated with the inhibition of global viral microRNAs, particularly the miR-BHRF1 cluster, and the high expression of SMAD3 , JUN , and COL1A genes...
February 14, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29444744/overexpression-of-pyruvate-dehydrogenase-e1a-subunit-inhibits-warburg-effect-and-induces-cell-apoptosis-through-mitochondria-mediated-pathway-in-hepatocellular-carcinoma
#3
Jihong Sun, Jingjing Li, Zhixian Guo, Lu Sun, Chenghui Juan, Yubing Zhou, Hongli Gu, Yan Yu, Qiuyue Hu, Quancheng' Kan, Zujiang Yu
Most cancers rely disproportionately on glycolysis for energy even in the presence of adequate oxygen supply, a condition known as "aerobic glycolysis", or the "Warburg effect". Pyruvate dehydrogenase E1a subunit (PDHA1) is one of main factors for metabolic switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis and has been suggested to be closely associated with tumorigenesis. Here we observed that PDHA1 protein was reduced in hepatocellular carcinoma (HCC) specimens by immunohistochemistry and western blot, which was significantly associated with poor overall survival...
February 14, 2018: Oncology Research
https://www.readbyqxmd.com/read/29427634/mucuna-pruriens-l-dc-chemo-sensitize-human-breast-cancer-cells-via-downregulation-of-prolactin-mediated-jak2-stat5a-signaling
#4
Sonam Sinha, Sonal Sharma, Jaykant Vora, Heta Shah, Anshu Srivastava, Neeta Shrivastava
ETHNOPHARMACOLOGICAL RELEVANCE: Mucuna pruriens (L.) DC (MP) is an ancient Indian medicinal plant traditionally used to treat Parkinson's disease. L-Dopa (LD), precursor of dopamine is abundantly found in the seeds of MP. L-dopa is a natural inhibitor of prolactin (PRL) hormone which is required to maintain lactation in women but it's over production (hyperprolactinemia) plays critical role in advancement of breast cancer. AIM OF THE STUDY: We aim to examine the pharmacological effect of LD and MP on this hyperprolactinemia associated breast cancer and related signaling for effective management of the disease...
February 7, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29427185/geriatric-nutritional-risk-index-as-a-prognostic-factor-in-patients-with-diffuse-large-b-cell-lymphoma
#5
Yusuke Kanemasa, Tatsu Shimoyama, Yuki Sasaki, Tsunekazu Hishima, Yasushi Omuro
The geriatric nutritional risk index (GNRI) is a simple and well-established nutritional assessment tool that is a significant prognostic factor for various cancers. However, the role of the GNRI in predicting clinical outcomes of diffuse large B cell lymphoma (DLBCL) patients has not been investigated. To address this issue, we retrospectively analyzed a total of 476 patients with newly diagnosed de novo DLBCL. We defined the best cutoff value of the GNRI as 96.8 using a receiver operating characteristic curve...
February 9, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29426376/silencing-of-nadph-oxidase-4-attenuates-hypoxia-resistance-in-neuroblastoma-cells-shsy-5y-by-inhibiting-pi3k-akt-dependent-glycolysis
#6
Ting Yu, Lei Li, Wenyan Liu, Bailiu Ya, Hongju Cheng, Qing Xin
Hypoxia-induced chemoresistance is a major obstacle in the development of effective cancer therapy. In our study, the reversal abilities of NADPH oxidase 4 (NOX4) silence on the hypoxia resistance and the potential mechanism were investigated. Our data showed that the expression of NOX4 was up-regulated in human neuroblastoma cells SHSY-5Y under hypoxia condition time-dependently. Knockdown of NOX4 expression by siRNA inhibited glycolysis induced by hypoxia through decreasing the expression of glycolysis related proteins (HIF-1α, LDHA, PDK1), decreasing glucose uptake, lactate production, ROS production while increasing mitochondria membrane potential...
February 9, 2018: Oncology Research
https://www.readbyqxmd.com/read/29425295/prenatal-exposure-to-unconventional-oil-and-gas-operation-chemical-mixtures-altered-mammary-gland-development-in-adult-female-mice
#7
Sarah A Sapouckey, Christopher D Kassotis, Susan C Nagel, Laura N Vandenberg
Unconventional oil and gas operations (UOG), which combine hydraulic fracturing (fracking) and directional drilling, involve the use of hundreds of chemicals including many with endocrine disrupting properties. Two previous studies examined mice exposed during early development to a 23-chemical mixture of UOG compounds (UOG-MIX) commonly used or produced in the process. Both male and female offspring exposed prenatally to one or more doses of UOG-MIX displayed alterations to endocrine organ function and serum hormone concentrations...
February 7, 2018: Endocrinology
https://www.readbyqxmd.com/read/29422776/different-tp53-mutants-in-p53-overexpressed-epithelial-ovarian-carcinoma-can-be-associated-both-with-altered-and-unaltered-glycolytic-and-apoptotic-profiles
#8
Stephanie Antoun, David Atallah, Roula Tahtouh, Nada Alaaeddine, Malak Moubarak, Abir Khaddage, Eliane Nasr Ayoub, George Chahine, George Hilal
Background: p53 is a tumor suppressor and key regulator of glycolysis in cancer cells, however highly mutated in tumors. In ovarian cancer, studies concerning p53 mutations focus on the DNA binding domain since the majority of hotspot mutations affects this region. Yet, mutations in other regions such as the proline rich domain may also affect the protein's expression and activity. The aim of this study is to investigate the effect of various positions of mutations in TP53 gene on glycolysis, apoptosis and transcription of p53 target genes...
2018: Cancer Cell International
https://www.readbyqxmd.com/read/29421654/extracellular-acidification-by-lactic-acid-suppresses-glucose-deprivation-induced-cell-death-and-autophagy-in-b16-melanoma-cells
#9
Taisuke Matsuo, Yasuyuki Sadzuka
In solid tumors, cancer cells survive and proliferate under conditions of microenvironment stress such as poor nutrients and hypoxia due to inadequate vascularization. These stress conditions in turn activate autophagy, which is important for cancer cell survival. However, autophagy has a contrary effect of inducing cell death in cancer cells cultured in vitro under conditions of glucose deprivation. In this study, we hypothesized that supplementation of lactic acid serves as a means of cell survival under glucose-deprived conditions...
February 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29416099/generation-and-characterization-of-a-igg-monoclonal-antibody-specific-for-gm3-neugc-ganglioside-by-immunizing-%C3%AE-3gn-t5-knockout-mice
#10
Dongwei He, Xiaoyan Fan, Boyi Liu, Yiqing Tian, Xiangmei Zhang, Lin Kang, Yan Tai, Shuzhen Liu, Qian Wang, Qingxia Li, Jianhui Cai
A murine monoclonal antibody (MAb-1) specific for GM3 has been generated by immunizing β3Gn-T5 knockout mice with purified GM3 ganglioside. The binding specificity of MAb-1 (IgG3 subclass) was established by an enzyme-linked immunosorbent assay (ELISA) and FACS and the antibody showed high binding specificity with GM3. Cell viability assay showed that MAb-1 significantly suppressed cell growth. Immunohistochemistry analysis revealed that MAb-1 was strongly expressed in human ovarian cancer tissues, whereas it was hardly expressed in normal tissues...
February 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29415045/linear-programming-model-can-explain-respiration-of-fermentation-products
#11
Philip Möller, Xiaochen Liu, Stefan Schuster, Daniel Boley
Many differentiated cells rely primarily on mitochondrial oxidative phosphorylation for generating energy in the form of ATP needed for cellular metabolism. In contrast most tumor cells instead rely on aerobic glycolysis leading to lactate to about the same extent as on respiration. Warburg found that cancer cells to support oxidative phosphorylation, tend to ferment glucose or other energy source into lactate even in the presence of sufficient oxygen, which is an inefficient way to generate ATP. This effect also occurs in striated muscle cells, activated lymphocytes and microglia, endothelial cells and several mammalian cell types, a phenomenon termed the "Warburg effect"...
2018: PloS One
https://www.readbyqxmd.com/read/29414630/development-and-validation-of-a-prognostic-index-for-survival-in-non-small-cell-lung-cancer-results-from-a-tunisian-cohort-study
#12
Ghassen Soussi, Nissaf Ben Alaya, Nawel Chaouch, Hajer Racil
INTRODUCTION: Despite the continuous efforts made with the TNM system, the issue of heterogeneity of prognosis within the stages of non-small cell lung cancer (NSCLC) could not be resolved. Our aim was to identify prognostic factors and develop an index to predict NSCLC survival with greater accuracy. METHODS: We conducted a survival study over 5 years on patients with NSCLC. Kaplan-Meier analysis followed by Cox regression modelling were used. Prognostic indices were derived, using either an additive or a multiplicative pattern, and were compared by their receiver operating characteristics (ROC) curves...
February 5, 2018: Cancer Epidemiology
https://www.readbyqxmd.com/read/29413596/an-aptamer-fe3-modified-nanoparticle-for-lactate-oxidation-and-tumor-photodynamic-therapy
#13
Huan Yang, Wen-Long Lu, Tao Huang, Qiu-Yun Chen, Jing Gao, Yao Zhao
To develop a cancer targeting lactate attenuator in vivo for cancer phototherapy and inhibition of HIF-1, we report an aptamer modified photo-responsive nanoparticle (labeled as Mn-D@BPFe-A) for lactate oxidation and cancer phototherapy. Mn-D@BPFe-A was constructed by the assembly of functional complex with BSA, followed by surface metal coordination and the recognition of Fe3+ with GAG containing sequence. Upon irradiation, Mn-D@BPFe-A NPs can oxidize water with the generation of OH, which convert lactate into pyruvate both in vitro and in vivo...
January 29, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29413509/evaluation-of-the-clinical-characteristics-of-everolimus-induced-lung-injury-and-determination-of-associated-risk-factors
#14
Mitsuhiro Abe, Kenji Tsushima, Jun Ikari, Naoko Kawata, Jiro Terada, Nobuhiro Tanabe, Koichiro Tatsumi
BACKGROUND: Everolimus (ERL), a mammalian target of rapamycin (mTOR) inhibitor, has been used for the management of several advanced cancers. ERL frequently causes lung injury, although the clinical and radiographic features have not been clarified. The aim of this study was to assess the clinical features of ERL-induced lung injury and determine the associated risk factors. METHODS: This single-center, retrospective study included 45 patients (29 men, 16 women; age, 12-78 years) who had received ERL at our hospital between August 2010 and March 2016...
January 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29412741/pharmacologic-reprogramming-designed-to-induce-a-warburg-effect-in-porcine-fetal-fibroblasts-alters-gene-expression-and-quantities-of-metabolites-from-conditioned-media-without-increased-cell-proliferation
#15
Bethany R Mordhorst, Stephanie L Murphy, Rence M Ross, Melissa S Samuel, Shirley Rojas Salazar, Tieming Ji, Susanta K Behura, Kevin D Wells, Jonathan A Green, Randall S Prather
The Warburg effect is a metabolic phenomenon characterized by increased glycolytic activity, decreased mitochondrial oxidative phosphorylation, and the production of lactate. This metabolic phenotype is characterized in rapidly proliferative cell types such as cancerous cells and embryonic stem cells. We hypothesized that a Warburg-like metabolism could be achieved in other cell types by treatment with pharmacological agents, which might, in turn, facilitate nuclear reprogramming. The aim of this study was to treat fibroblasts with CPI-613 and PS48 to induce a Warburg-like metabolic state...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29411725/vascular-endothelial-growth-factor-in-pleural-effusions-and-correlation-with-radiologic-and-biochemical-parameters
#16
N Bayram, Y Karakan, M Uyar, B Ozyurt, A Filiz
INTRODUCTION: Pleural effusion is a common clinical problem with management difficulties. The aim of this study is to evaluate vascular endothelial growth factor (VEGF) in differential diagnosis of pleural effusions and the presence of correlation between radiological features and biochemical properties. MATERIALS AND METHODS: The study included patients with pleural effusion. VEGF levels in the pleural fluid were measured by enzyme-linked immunosorbent assay. RESULTS: A total of 97 patients who had exudative pleural effusion related to lung cancer (n = 17), nonpulmonary malignancies (n = 25), mesothelioma (n = 9), pneumonia (n = 14), tuberculosis (n = 8), miscellaneous causes (n = 6), and transudative effusion (n = 18) were included...
January 2018: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29404815/intra-cellular-lactate-concentration-in-t-lymphocytes-from-septic-shock-patients-a-pilot-study
#17
Thibaut Girardot, Thomas Rimmelé, Guillaume Monneret, Julien Textoris, Fabienne Venet
BACKGROUND: Sepsis-associated hyperlactatemia is a widely used biomarker, associated with initial severity and poor outcomes. This increased circulating lactate concentration has been proposed to result in part from a mismatch between oxygen delivery and demand in organs. However, other mechanisms may participate. In particular, a metabolic reprogramming similar to the Warburg effect initially described in cancer cells could lead to increased lactate production by immune cells such as T lymphocytes after sepsis...
February 5, 2018: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/29400636/buformin-suppresses-proliferation-and-invasion-via-ampk-s6-pathway-in-cervical-cancer-and-synergizes-with-paclitaxel
#18
Jing Li, Ling Chen, Qiuli Liu, Mei Tang, Yuan Wang, Jinjin Yu
Buformin is an old anti-diabetic agent and manifests potent anti-tumor activities in several malignancies. In the present study, we aimed to explore the functions of buformin in human cervical cancer. As our data shown, buformin exhibited significant anti-proliferative effects in a dose-dependent manner in 4 cervical cancer cell lines. Compared to the control, buformin notably suppressed colony formation and increased ROS production in C33A, Hcc94 and SiHa cells. Flow cytometric analysis showed that buformin induced marked cell cycle arrest but only resulted in mild apoptosis...
February 5, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29396666/cross-reactivity-between-muc1-antigen-and-mca-false-elevation-of-serum-ca-15-3-level-in-pregnant-and-lactating-women-by-ma695-ma552-based-assay
#19
Guangshu Liang, Xuqian Fang, Xiaoyi Lin, Xiaojing Feng, Huangying Lu, Yinglei Wan, Zhidong Gu
PURPOSE: Cancer antigen 153 (CA 15-3) is one of the most commonly used biomarkers of breast cancer. However, elevated CA 15-3 is reported in pregnant and lactating women more frequently on Beckman DxI 800 immunoassay system (Ma695-Ma552 antibody pair) than on Abbott ARCHITECT system (115D8-DF3 antibody pair) in laboratory methodological evaluation. We conducted this study in order to figure out the reason behind this phenomenon. METHODS: Serum CA 15-3 concentration was analyzed in 426 subjects, including 180 patients with breast cancer, 121 patients with benign breast disease, and 125 healthy volunteers (45 pregnant and 80 non-pregnant women)...
February 2, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29396322/a-landscape-of-metabolic-variation-across-tumor-types
#20
Ed Reznik, Augustin Luna, Bülent Arman Aksoy, Eric Minwei Liu, Konnor La, Irina Ostrovnaya, Chad J Creighton, A Ari Hakimi, Chris Sander
Tumor metabolism is reorganized to support proliferation in the face of growth-related stress. Unlike the widespread profiling of changes to metabolic enzyme levels in cancer, comparatively less attention has been paid to the substrates/products of enzyme-catalyzed reactions, small-molecule metabolites. We developed an informatic pipeline to concurrently analyze metabolomics data from over 900 tissue samples spanning seven cancer types, revealing extensive heterogeneity in metabolic changes relative to normal tissue across cancers of different tissues of origin...
January 26, 2018: Cell Systems
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