keyword
MENU ▼
Read by QxMD icon Read
search

Microphysiological system

keyword
https://www.readbyqxmd.com/read/29141507/sandwiched-white-adipose-tissue-a-novel-microphysiological-system-of-human-adipose-tissue
#1
Frank H Lau, Kelly Vogel, John P Luckett, Maxwell Hunt, Alicia Meyer, Camille L Rogers, Oren Tessler, Charles L Dupin, Hugo St Hilaire, Kazi N Islam, Trivia Frazier, Jeffrey Gimble, Steven Scahill
White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful tissue culture models of primary human WAT remain limited, at best. Here we describe a novel WAT culture system in which primary human WAT is sandwiched between tissue-engineered sheets of adipose-derived stromal cells. This construct, called "sandwiched white adipose tissue" (SWAT), can be defined as a Microphysiological System (MPS) since it is a tissue-engineered, multicellular, 3D organ construct produced using human cells...
November 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29137633/bi-directional-exosome-driven-intercommunication-between-the-hepatic-niche-and-cancer-cells
#2
Nikolina Dioufa, Amanda M Clark, Bo Ma, Colin H Beckwitt, Alan Wells
BACKGROUND: Our understanding of the multiple roles exosomes play during tumor progression is still very poor and the contribution of the normal tissue derived exosomes in distant seeding and tumor outgrowth has also not been widely appreciated. METHODS: Using our all-human liver microphysiological system (MPS) platform as a model to closely recapitulate the early metastatic events, we isolated exosomes from both tumor cells and liver microenvironment. RESULTS: We observed that while priming of the hepatic niche (HepN) with MDA-231 breast cancer derived exosomes facilitated seeding of the cancer cells in the liver, subsequent tumor outgrowth was diminished; this was consistent with increased entry into dormancy...
November 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/29128921/a-microfluidic-in-line-elisa-for-measuring-secreted-protein-under-perfusion
#3
Qiyue Luan, Stacey Cahoon, Agnes Wu, Shyam Sundhar Bale, Martin Yarmush, Abhinav Bhushan
Recent progress in the development of microfluidic microphysiological systems such as 'organs-on-chips' and microfabricated cell culture is geared to simulate organ-level physiology. These tissue models leverage microengineering technologies that provide capabilities of presenting cultured cells with input signals in a more physiologically relevant context such as perfused flow. Proteins that are secreted from cells have important information about the health of the cells. Techniques to quantify cellular proteins include mass spectrometry to ELISA (enzyme-linked immunosorbent assay)...
November 11, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/29121458/placental-drug-transport-on-a-chip-a-microengineered-in-vitro-model-of-transporter-mediated-drug-efflux-in-the-human-placental-barrier
#4
Cassidy Blundell, Yoon-Suk Yi, Lin Ma, Emily R Tess, Megan J Farrell, Andrei Georgescu, Lauren M Aleksunes, Dongeun Huh
The current lack of knowledge about the effect of maternally administered drugs on the developing fetus is a major public health concern worldwide. The first critical step toward predicting the safety of medications in pregnancy is to screen drug compounds for their ability to cross the placenta. However, this type of preclinical study has been hampered by the limited capacity of existing in vitro and ex vivo models to mimic physiological drug transport across the maternal-fetal interface in the human placenta...
November 9, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29065799/fitting-tissue-chips-and-microphysiological-systems-into-the-grand-scheme-of-medicine-biology-pharmacology-and-toxicology
#5
David E Watson, Rosemarie Hunziker, John P Wikswo
Microphysiological systems (MPS), which include engineered organoids (EOs), single organ/tissue chips (TCs), and multiple organs interconnected to create miniature in vitro models of human physiological systems, are rapidly becoming effective tools for drug development and the mechanistic understanding of tissue physiology and pathophysiology. The second MPS thematic issue of Experimental Biology and Medicine comprises 15 articles by scientists and engineers from the National Institutes of Health, the IQ Consortium, the Food and Drug Administration, and Environmental Protection Agency, an MPS company, and academia...
October 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29065798/microphysiologic-systems-in-female-reproductive-biology
#6
REVIEW
Alexandria N Young, Georgette Moyle-Heyrman, J Julie Kim, Joanna E Burdette
Microphysiologic systems (MPS), including new organ-on-a-chip technologies, recapitulate tissue microenvironments by employing specially designed tissue or cell culturing techniques and microfluidic flow. Such systems are designed to incorporate physiologic factors that conventional 2D or even 3D systems cannot, such as the multicellular dynamics of a tissue-tissue interface or physical forces like fluid sheer stress. The female reproductive system is a series of interconnected organs that are necessary to produce eggs, support embryo development and female health, and impact the functioning of non-reproductive tissues throughout the body...
November 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29065797/self-contained-low-cost-body-on-a-chip-systems-for-drug-development
#7
REVIEW
Ying I Wang, Carlota Oleaga, Christopher J Long, Mandy B Esch, Christopher W McAleer, Paula G Miller, James J Hickman, Michael L Shuler
Integrated multi-organ microphysiological systems are an evolving tool for preclinical evaluation of the potential toxicity and efficacy of drug candidates. Such systems, also known as Body-on-a-Chip devices, have a great potential to increase the successful conversion of drug candidates entering clinical trials into approved drugs. Systems, to be attractive for commercial adoption, need to be inexpensive, easy to operate, and give reproducible results. Further, the ability to measure functional responses, such as electrical activity, force generation, and barrier integrity of organ surrogates, enhances the ability to monitor response to drugs...
November 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29065796/circadian-hormone-control-in-a-human-on-a-chip-in-vitro-biology-s-ignored-component
#8
REVIEW
Kevin J Cyr, Omero M Avaldi, John P Wikswo
Organs-on-Chips (OoCs) are poised to reshape dramatically the study of biology by replicating in vivo the function of individual and coupled human organs. Such microphysiological systems (MPS) have already recreated complex physiological responses necessary to simulate human organ function not evident in two-dimensional in vitro biological experiments. OoC researchers hope to streamline pharmaceutical development, accelerate toxicology studies, limit animal testing, and provide new insights beyond the capability of current biological models...
November 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29058990/introduction-to-the-theme-new-approaches-for-studying-drug-and-toxicant-action-applications-to-drug-discovery-and-development
#9
Paul A Insel, Susan G Amara, Terrence F Blaschke, Urs A Meyer
The theme "New Approaches for Studying Drug and Toxicant Action: Applications to Drug Discovery and Development" links 13 articles in this volume of the Annual Review of Pharmacology and Toxicology (ARPT). The engaging prefatory articles by Arthur Cho and Robert Lefkowitz set the stage for this theme and for the reviews that insightfully describe new approaches that advance research and discovery in pharmacology and toxicology. Examples include the progress being made in developing Organson- Chips/microphysiological systems and human induced pluripotent stem cell (iPSC)-derived cells to aid in understanding cell and tissue pharmacokinetics, action, and toxicity; the recognition of the importance of circadian rhythm, the microbiome, and epigenetics in drug and toxicant responses; and the application of results from new types of patient-derived information to create personalized/precision medicine, including therapeutics for pain, which may perhaps provide help in dealing with the opioid epidemic in the United States...
October 20, 2017: Annual Review of Pharmacology and Toxicology
https://www.readbyqxmd.com/read/29029591/application-of-microphysiological-systems-to-enhance-safety-assessment-in-drug-discovery
#10
Lorna Ewart, Eva-Maria Dehne, Kristin Fabre, Susan Gibbs, James Hickman, Ellinor Hornberg, Magnus Ingelman-Sundberg, Kyung-Jin Jang, David R Jones, Volker M Lauschke, Uwe Marx, Jerome T Mettetal, Amy Pointon, Dominic Williams, Wolfram-Hubertus Zimmermann, Peter Newham
Enhancing the early detection of new therapies that are likely to carry a safety liability in the context of the intended patient population would provide a major advance in drug discovery. Microphysiological systems (MPS) technology offers an opportunity to support enhanced preclinical to clinical translation through the generation of higher-quality preclinical physiological data. In this review, we highlight this technological opportunity by focusing on key target organs associated with drug safety and metabolism...
October 13, 2017: Annual Review of Pharmacology and Toxicology
https://www.readbyqxmd.com/read/28985032/organ-on-a-chip-systems-for-women-s-health-applications
#11
REVIEW
Janna Nawroth, Julia Rogal, Martin Weiss, Sara Y Brucker, Peter Loskill
Biomedical research, for a long time, has paid little attention to the influence of sex in many areas of study, ranging from molecular and cellular biology to animal models and clinical studies on human subjects. Many studies solely rely on male cells/tissues/animals/humans, although there are profound differences in male and female physiology, which can significantly impact disease mechanisms, toxicity of compounds, and efficacy of pharmaceuticals. In vitro systems have been traditionally very limited in their capacity to recapitulate female-specific physiology and anatomy such as dynamic sex-hormone levels and the complex interdependencies of female reproductive tract organs...
October 6, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28976521/cardiac-microphysiological-devices-with-flexible-thin-film-sensors-for-higher-throughput-drug-screening
#12
Johan U Lind, Moran Yadid, Ian Perkins, Blakely B O'Connor, Feyisayo Eweje, Christophe O Chantre, Matthew A Hemphill, Hongyan Yuan, Patrick H Campbell, Joost J Vlassak, Kevin K Parker
Microphysiological systems and organs-on-chips promise to accelerate biomedical and pharmaceutical research by providing accurate in vitro replicas of human tissue. Aside from addressing the physiological accuracy of the model tissues, there is a pressing need for improving the throughput of these platforms. To do so, scalable data acquisition strategies must be introduced. To this end, we here present an instrumented 24-well plate platform for higher-throughput studies of engineered human stem cell-derived cardiac muscle tissues that recapitulate the laminar structure of the native ventricle...
October 25, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28884006/engineering-challenges-in-microphysiological-systems
#13
EDITORIAL
Yu Shrike Zhang
No abstract text is available yet for this article.
August 2017: Future Science OA
https://www.readbyqxmd.com/read/28800376/fetal-bovine-serum-fbs-past-present-future
#14
Jan van der Valk, Karen Bieback, Christiane Buta, Brett Cochrane, Wilhelm G Dirks, Jianan Fu, James J Hickman, Christiane Hohensee, Roman Kolar, Manfred Liebsch, Francesca Pistollato, Markus Schulz, Daniel Thieme, Tilo Weber, Joachim Wiest, Stefan Winkler, Gerhard Gstraunthaler
The supplementation of culture medium with fetal bovine serum (FBS, also referred to as 'fetal calf serum') is still common practice in cell culture applications. Due to a number of disadvantages in terms of quality and reproducibility of in vitro data, animal welfare concerns, and in light of recent cases of fraudulent marketing, the search for alternatives and the development of serum-free medium formulations gained global attention. Here, we report on the 3rd Workshop on FBS, Serum Alternatives and Serum-free Media, where (a) regulatory aspects, (b) the serum dilemma, (c) alternatives to FBS, (d) case-studies of serum-free in vitro applications, and (e) the establishment of serum-free databases, were discussed...
August 9, 2017: ALTEX
https://www.readbyqxmd.com/read/28795174/tissue-chips-innovative-tools-for-drug-development-and-disease-modeling
#15
REVIEW
L A Low, D A Tagle
The high rate of failure during drug development is well-known, however recent advances in tissue engineering and microfabrication have contributed to the development of microphysiological systems (MPS), or 'organs-on-chips' that recapitulate the function of human organs. These 'tissue chips' could be utilized for drug screening and safety testing to potentially transform the early stages of the drug development process. They can also be used to model disease states, providing new tools for the understanding of disease mechanisms and pathologies, and assessing effectiveness of new therapies...
September 12, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28777442/microphysiological-systems-to-assess-nonclinical-toxicity
#16
Kirk P Van Ness, Shih-Yu Chang, Elijah J Weber, Danielle Zumpano, David L Eaton, Edward J Kelly
The liver and the kidney are key toxicity target organs during drug development campaigns, as they typically carry the burden of drug transport and metabolism. Primary hepatocytes and proximal tubule epithelial cells grown in traditional in vitro 2-D culture systems do not maintain transporter and metabolic functions, thus limiting their utility for nonclinical toxicology investigations. We have developed a renal and hepatic microphysiological system (MPS) platform that uses a commercially available MPS device as the core cell culture platform for our methodologies...
August 4, 2017: Current Protocols in Toxicology
https://www.readbyqxmd.com/read/28752430/integrated-gut-and-liver-microphysiological-systems-for-quantitative-in-vitro-pharmacokinetic-studies
#17
Nikolaos Tsamandouras, Wen Li Kelly Chen, Collin D Edington, Cynthia L Stokes, Linda G Griffith, Murat Cirit
Investigation of the pharmacokinetics (PK) of a compound is of significant importance during the early stages of drug development, and therefore several in vitro systems are routinely employed for this purpose. However, the need for more physiologically realistic in vitro models has recently fueled the emerging field of tissue-engineered 3D cultures, also referred to as organs-on-chips, or microphysiological systems (MPSs). We have developed a novel fluidic platform that interconnects multiple MPSs, allowing PK studies in multi-organ in vitro systems along with the collection of high-content quantitative data...
July 27, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28670478/tailoring-cardiac-environment-in-microphysiological-systems-an-outlook-on-current-and-perspective-heart-on-chip-platforms
#18
EDITORIAL
Giovanni Stefano Ugolini, Roberta Visone, Daniela Cruz-Moreira, Alberto Redaelli, Marco Rasponi
No abstract text is available yet for this article.
June 2017: Future Science OA
https://www.readbyqxmd.com/read/28670475/the-ascendance-of-microphysiological-systems-to-solve-the-drug-testing-dilemma
#19
REVIEW
Eva-Maria Dehne, Tobias Hasenberg, Uwe Marx
The development of drugs is a process obstructed with manifold security and efficacy concerns. Although animal models are still widely used to meet the diligence required, they are regarded as outdated tools with limited predictability. Novel microphysiological systems intend to create systemic models of human biology. Their ability to host 3D organoid constructs in a controlled microenvironment with mechanical and electrophysiological stimuli enables them to create and maintain homeostasis. These platforms are, thus, envisioned to be superior tools for testing and developing substances such as drugs, cosmetics and chemicals...
June 2017: Future Science OA
https://www.readbyqxmd.com/read/28667746/integrated-gut-liver-microphysiological-systems-elucidates-inflammatory-inter-tissue-crosstalk
#20
Wen L K Chen, Collin Edington, Emily Suter, Jiajie Yu, Jeremy J Velazquez, Jason G Velazquez, Michael Shockley, Emma M Large, Raman Venkataramanan, David J Hughes, Cynthia L Stokes, David L Trumper, Rebecca L Carrier, Murat Cirit, Linda G Griffith, Douglas A Lauffenburger
A capability for analyzing complex cellular communication among tissues is important in drug discovery and development, and in vitro technologies for doing so are required for human applications. A prominent instance is communication between the gut and the liver, whereby perturbations of one tissue can influence behavior of the other. Here, we present a study on human gut-liver tissue interactions under normal and inflammatory contexts, via an integrative multi-organ platform comprising human liver (hepatocytes and Kupffer cells), and intestinal (enterocytes, goblet cells, and dendritic cells) models...
November 2017: Biotechnology and Bioengineering
keyword
keyword
109656
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"