N Pozzi, L Acquasaliente, R Frasson, A Cristiani, S Moro, A Banzato, V Pengo, G L Scaglione, A Arcovito, R De Cristofaro, V De Filippis
BACKGROUND: This work was aimed at characterizing the interaction of β(2)-glycoprotein I (β(2)GPI), an abundant plasma protein of unknown function, with human thrombin, the final effector protease in the coagulation cascade. METHODS: The β(2)GPI-thrombin interaction was studied by surface plasmon resonance (SPR), fluorescence, and molecular modeling. The effect of β(2)GPI on the procoagulant (fibrin generation and platelet aggregation) and anticoagulant (protein C activation) functions of thrombin were investigated with turbidimetric, immunocytofluorimetric and enzymatic assays...
June 2013: Journal of Thrombosis and Haemostasis: JTH