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https://www.readbyqxmd.com/read/28193827/monocyte-subsets-coregulate-inflammatory-responses-by-integrated-signaling-through-tnf-and-il-6-at-the-endothelial-cell-interface
#1
Myriam Chimen, Clara M Yates, Helen M McGettrick, Lewis S C Ward, Matthew J Harrison, Bonita Apta, Lea H Dib, Beat A Imhof, Paul Harrison, Gerard B Nash, G Ed Rainger
Two major monocyte subsets, CD14(+)CD16(-) (classical) and CD14(+/dim)CD16(+) (nonclassical/intermediate), have been described. Each has different functions ascribed in its interactions with vascular endothelial cells (EC), including migration and promoting inflammation. Although monocyte subpopulations have been studied in isolated systems, their influence on EC and on the course of inflammation has been ignored. In this study, using unstimulated or cytokine-activated EC, we observed significant differences in the recruitment, migration, and reverse migration of human monocyte subsets...
February 13, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28192188/hepatitis-c-virus-induced-nk-cell-activation-causes-metzincin-mediated-cd16-cleavage-and-impaired-antibody-dependent-cytotoxicity
#2
Barbara Oliviero, Stefania Mantovani, Stefania Varchetta, Dalila Mele, Giulia Grossi, Serena Ludovisi, Elisa Nuti, Armando Rossello, Mario U Mondelli
BACKGROUND & AIMS: The Fc receptor family for IgG type III (FcγRIII, CD16) is an activating receptor on natural killer (NK) cells and an essential mediator of antibody-dependent cellular cytotoxicity (ADCC). There is only limited information on its role during chronic hepatitis C virus (HCV) infection. We studied CD16 expression in relation to NK cell functional activity in HCV-infected patients and sought mechanistic insights into virus-induced modulation. METHODS: NK cell CD16 expression and activation status were evaluated ex vivo by flow cytometry in HCV-infected patients and healthy controls (HC) as well as in vitro after co-culture with HCV-infected Huh 7...
February 9, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28183821/exercise-induced-upregulation-of-endothelial-adhesion-molecules-in-human-skeletal-muscle-and-number-of-circulating-cells-with-remodeling-properties
#3
Anna Strömberg, Eric Rullman, Eva Jansson, Thomas Gustafsson
Multipotent cells have received great interest due to their potential capacity to repair and remodel peripheral tissues. We examined the effect of an acute exercise bout on the number of circulating cells with known remodeling properties, and the level of factors in plasma and skeletal muscle tissue with potential to recruit these cells. Twenty healthy male subjects performed a 60 min cycling exercise. Blood samples for flow cytometry were drawn from 10 subjects (Group 1) before and up to 2 h after exercise, and absolute cell counts of the classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(++)) monocyte (MO) subpopulations, and of CD45(dim)CD34(+)VEGFR2(+) endothelial progenitor cells (EPCs) were measured using bead-based determination...
February 9, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28183683/study-of-cd4-cd8-and-natural-killer-cells-cd16-cd56-in-children-with-immune-thrombocytopenic-purpura
#4
Farida Hussein El-Rashedy, Mahmoud Ahmed El-Hawy, Mohamed Ahmed Helwa, Sameh Said Abd-Allah
OBJECTIVE/BACKGROUND: To assess the percentage of CD4(+), CD8(+), and natural killer cells (CD16(+), CD56(+)) in children with immune thrombocytopenic purpura (ITP) at presentation and study their impact on disease chronicity. METHODS: This case-control study was conducted at the Pediatric Hematology and Oncology Unit, Menoufia University Hospital (tertiary care center in Egypt). The study was held on 30 children presenting with ITP; they were followed-up and classified into two groups: 15 children with acute ITP; and 15 children with chronic ITP...
February 1, 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/28183329/cd14-bright-cd16-intermediate-monocytes-are-induced-by-interleukin-10-and-positively-correlate-with-disease-activity-in-rheumatoid-arthritis
#5
Masako Tsukamoto, Noriyuki Seta, Keiko Yoshimoto, Katsuya Suzuki, Kunihiro Yamaoka, Tsutomu Takeuchi
BACKGROUND: Three different subsets of circulating human monocytes, CD14(bright)CD16- (classical), CD14(bright)CD16+ (intermediate), and CD14(dim)CD16+ (non-classical) have been recently identified. It has been reported that CD14(bright)CD16+ monocytes are increased in rheumatoid arthritis (RA). However, the role of each monocyte subset in the pathogenesis of RA is still unclear. The purpose of this study was to investigate the association of CD14(bright)CD16+ monocytes with RA. METHODS: The study enrolled 35 patients with RA and 14 healthy volunteers...
February 10, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28170096/circulating-nk-cells-and-their-subsets-in-beh%C3%A3-et-s-disease
#6
Md Samiul Hasan, Paul L Ryan, Lesley A Bergmeier, Farida Fortune
Behçet's Disease (BD) is an auto-inflammatory, chronic relapsing/remitting disease of unknown aetiology with both innate and acquired immune cells implicated in disease pathogenesis. Peripheral blood Natural Killer (NK) cells and their CD56(Dim) /CD56(Bright) subsets were surface phenotyped using CD27 and CD16 surface markers in 60 BD patients compared to 60 healthy controls (HCs). Functional potential was assessed by production of interferon (IFN)-γ, Granzyme B, Perforin and the expression of degranulation marker CD107a...
February 7, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28152331/comparison-of-immunohematological-profile-between-endurance-and-power-oriented-elite-athletes
#7
José P Morgado, Catarina N Matias, Cristina P Monteiro, Francisco Alves, Joana F Reis, Diana A Santos, Analiza M Silva, Fátima Martins, Maria T Seixas, Petronila Rocha-Pereira, Luís B Sardinha, Maria J Laires
There is general perception that elite athletes are highly susceptible to changes in immunohematological profile. The objective of this study was to compare immunohematological parameters of elite athletes of different aerobic and muscular strength sports and analyze changes over 2 months. Sixteen judoists and 14 swimmers were evaluated 2 months before (M1) and immediately prior to competition (M2). Hemogram and lymphocytes subpopulations were assessed with automatic counter and flow cytometry, respectively...
November 9, 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28151065/relative-mrna-expression-levels-of-restriction-factors-and-antiviral-genes-in-fetal-and-adult-human-monocytes-and-monocyte-derived-macrophages
#8
Karen Trang, Rui André Saraiva Raposo, Margaret M Lowe, Elisabeth R Krow-Lucal, Wes Yonemoto, Vinicius D Cabido, Devi SenGupta, Joseph M McCune
Among untreated HIV-infected pregnant women, the frequency of mother-to-child transmission of HIV is low (5-10%), with most infections occurring at or after birth. Given findings that fetal and adult monocytes are distinct from one another in terms of basal transcriptional profiles, and in phosphorylation of signal transducer and activators of transcription in response to cytokines, we hypothesized that fetal CD14+CD16- monocyte and monocyte-derived macrophages (MDMs) might, compared to their adult counterparts, express higher levels of transcripts for restriction factors and antiviral factors at baseline and/or after stimulation with cytokines that might be induced upon transmission of HIV in utero, for example, IFNα, IFNγ, and IL-6...
February 2, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28148734/cutting-edge-helicobacter-pylori-induces-nuclear-hypersegmentation-and-subtype-differentiation-of-human-neutrophils-in-vitro
#9
Laura C Whitmore, Megan N Weems, Lee-Ann H Allen
Helicobacter pylori infects the human stomach and causes a spectrum of disease that includes gastritis, peptic ulcers, and gastric adenocarcinoma. A chronic, neutrophil-rich inflammatory response characterizes this infection. It is established that H. pylori stimulates neutrophil chemotaxis and a robust respiratory burst, but other aspects of this interaction are incompletely defined. We demonstrate here that H. pylori induces N1-like subtype differentiation of human neutrophils as indicated by profound nuclear hypersegmentation, a CD62L(dim), CD16(bright), CD11b(bright), CD66b(bright), CD63(bright) surface phenotype, proinflammatory cytokine secretion, and cytotoxicity...
February 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28138156/antibody-dependent-cell-cytotoxicity-adcc-immunotherapy-strategies-enhancing-effector-nk-cells
#10
REVIEW
Maria Carmen Ochoa, Luna Minute, Inmaculada Rodriguez, Saray Garasa, Elisabeth Perez-Ruiz, Susana Inogés, Ignacio Melero, Pedro Berraondo
Antibody-dependent cellular cytotoxicity (ADCC) is a set of mechanisms that target cells coated with IgG antibodies of the proper subclasses (IgG1 in the human) to be the prey of cell-to-cell cytolysis executed by immune cells expressing FcRIIIA (CD16A). These effectors include not only NK cells but also other CD16(+) subsets such as monocyte/macrophages, NKT cells or γδ T cells. In cancer therapy, ADCC is exploited by antibodies that selectively recognize proteins on the surface of malignant cells. An approach to enhance antitumor activity is to act on effector cells so they are increased in their numbers or enhanced in their individual (on a cell per cell basis) ADCC performance...
January 31, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28135772/evidence-for-a-role-of-eosinophils-in-blister-formation-in-bullous-pemphigoid
#11
Elisabeth de Graauw, Cassian Sitaru, Michael Horn, Luca Borradori, Shida Yousefi, Hans-Uwe Simon, Dagmar Simon
BACKGROUND: Bullous pemphigoid (BP) is an autoimmune bullous disease of the skin characterized by subepidermal blister formation due to tissue-bound and circulating autoantibodies to the hemidesmosomal antigens BP180 and BP230. Although eosinophils and their toxic mediators are found abundantly in BP lesions, their role in blister formation has remained unclear. OBJECTIVE: To investigate the role of eosinophils in the pathogenesis of BP with a specific focus on blister formation and to define conditions inducing dermal-epidermal separation (DES)...
January 30, 2017: Allergy
https://www.readbyqxmd.com/read/28133918/the-clinicopathologic-significance-of-lymphocyte-subsets-in-acute-myeloid-leukemia
#12
M Alcasid, L Ma, J R Gotlib, D A Arber, R S Ohgami
INTRODUCTION: While the role of the immune system in altering and modulating the progression of solid tumors is well studied, the impact of the immune system on the outcome and progression of hematolymphoid neoplasms is still poorly understood. METHODS: Here, we report a retrospective study detailing our analysis of 130 patients with acute myeloid leukemia (AML), with flow cytometry immunophenotypic evaluation of major lymphocyte subsets including B cells, T cells, and NK cells...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28133717/dopamine-increases-cd14-cd16-monocyte-transmigration-across-the-blood-brain-barrier-implications-for-substance-abuse-and-hiv-neuropathogenesis
#13
Tina M Calderon, Dionna W Williams, Lillie Lopez, Eliseo A Eugenin, Laura Cheney, Peter J Gaskill, Mike Veenstra, Kathryn Anastos, Susan Morgello, Joan W Berman
In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14(+)CD16(+) monocytes mediate HIV entry into the central nervous system (CNS) and that uninfected and infected CD14(+)CD16(+) monocyte transmigration across the blood brain barrier (BBB) contributes to the establishment and propagation of CNS HIV viral reservoirs and chronic neuroinflammation, important factors in the development of HAND...
January 29, 2017: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
https://www.readbyqxmd.com/read/28131632/cd56-enriched-donor-cell-infusion-after-post-transplantation-cyclophosphamide-for-haploidentical-transplantation-of-advanced-myeloid-malignancies-is-associated-with-prompt-reconstitution-of-mature-natural-killer-cells-and-regulatory-t-cells-with-reduced-incidence
#14
Sarita Rani Jaiswal, Shamsur Zaman, Murugaiyan Nedunchezhian, Aditi Chakrabarti, Prakash Bhakuni, Margoob Ahmed, Kanika Sharma, Sheh Rawat, Paul O'donnell, Suparno Chakrabarti
We conducted a pilot study on the feasibility of CD56-enriched donor cell infusion after post-transplantation cyclophosphamide (PTCy) for 10 patients with advanced myeloid malignancies undergoing haploidentical peripheral blood stem cell transplantation with cyclosporine alone as graft-versus-host disease (GVHD) prophylaxis and compared the outcome and immune reconstitution with a control group of 20 patients undergoing the same without CD56-enriched donor cell infusion. An early and rapid surge of mature NK cells as well as CD4(+) T cells and regulatory T cells (Tregs) was noted compared with the control group...
January 25, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28130912/effects-on-human-heart-valve-immunogenicity-in-vitro-by-high-concentration-cryoprotectant-treatment
#15
Benjamin A Högerle, Maria Schneider, Katrin Sudrow, Naima Souidi, Meaghan Stolk, Isabella Werner, Anna Biermann, Kelvin G M Brockbank, Ulrich A Stock, Martina Seifert
Previously it was shown that cryopreservation using an ice-free cryopreservation method with the cryoprotectant formulation VS83 beneficially modulated immune reactions in vivo and in vitro when compared with conventionally frozen tissues. In this study, we assessed the impact of a VS83 post-treatment of previously conventionally frozen human tissue on responses of human immune cells in vitro. Tissue punches of treated and non-treated (control) aortic heart valve tissue (leaflets and associated aortic root) were co-cultured for 7 days with peripheral blood mononuclear cells or enriched CD14(+) monocytes...
January 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28126036/genome-wide-analysis-of-differential-transcriptional-and-epigenetic-variability-across-human-immune-cell-types
#16
Simone Ecker, Lu Chen, Vera Pancaldi, Frederik O Bagger, José María Fernández, Enrique Carrillo de Santa Pau, David Juan, Alice L Mann, Stephen Watt, Francesco Paolo Casale, Nikos Sidiropoulos, Nicolas Rapin, Angelika Merkel, Hendrik G Stunnenberg, Oliver Stegle, Mattia Frontini, Kate Downes, Tomi Pastinen, Taco W Kuijpers, Daniel Rico, Alfonso Valencia, Stephan Beck, Nicole Soranzo, Dirk S Paul
BACKGROUND: A healthy immune system requires immune cells that adapt rapidly to environmental challenges. This phenotypic plasticity can be mediated by transcriptional and epigenetic variability. RESULTS: We apply a novel analytical approach to measure and compare transcriptional and epigenetic variability genome-wide across CD14(+)CD16(-) monocytes, CD66b(+)CD16(+) neutrophils, and CD4(+)CD45RA(+) naïve T cells from the same 125 healthy individuals. We discover substantially increased variability in neutrophils compared to monocytes and T cells...
January 26, 2017: Genome Biology
https://www.readbyqxmd.com/read/28122717/acute-high-intensity-interval-exercise-reduces-human-monocyte-toll-like-receptor-2-expression-in-type-2-diabetes
#17
Cody Durrer, Monique Emily Francois, Helena Neudorf, Jonathan Peter Little
: Type 2 diabetes (T2D) is characterized by chronic low-grade inflammation that contributes to disease pathophysiology. Exercise has anti-inflammatory effects but the impact of high-intensity interval training (HIIT) is not known. PURPOSE: To determine the impact of a single session of HIIT on cellular, molecular, and circulating markers of inflammation in individuals with T2D. METHODS: Participants with T2D (n=10) and healthy age-matched controls (HC; n=9) completed an acute bout of HIIT (7 X 1-min @ ~85% maximal aerobic power output, separated by 1-min recovery) on a cycle ergometer with blood samples obtained before (Pre), immediately after (Post), and at one-hour of recovery (1-h Post)...
January 25, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28108365/neutrophil-subset-responses-in-infants-with-severe-viral-respiratory-infection
#18
Bart Cortjens, Sarah A Ingelse, Job C Calis, Alexander P Vlaar, Leo Koenderman, Reinout A Bem, Job B van Woensel
Neutrophils are the predominant inflammatory cells recruited to the respiratory tract as part of the innate immune response to viral infections. Recent reports indicate the existence of distinct functional neutrophil subsets in the circulatory compartment of adults, following severe inflammatory conditions. Here, we evaluated the occurrence of neutrophil subsets in blood and broncho-alveolar lavage fluid during severe viral respiratory infection in infants based on CD16/CD62L expression. We show that during the course of severe respiratory infection infants may develop four heterogeneous neutrophil subsets in blood (mature, immature, progenitor, and suppressive neutrophils), each with distinct activation states...
January 17, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28105088/clinical-significance-of-lymphocyte-subset-changes-in-hemophagocytic-lymphohistiocytosis-of-children
#19
Qi An, Yi Wang, Shaoyan Hu, Daihua Fang, Chengmin Xuan, Shumei Xu, Mingwei Jin, Qiang Ji
In order to examine the role of peripheral blood lymphocyte subsets on the diagnosis, treatment and prognosis of hemophagocytic lymphohistiocytosis (HLH), 30 affected children during the acute period of the disease and 30 healthy children within the same age range were selected to test their peripheral blood lymphocyte subsets using flow cytometry and compare these subsets. At the same time, the peripheral blood lymphocyte subsets of 20 children with complete remission from HLH were compared to those of 10 cases who succumbed to the disease...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28100681/lfa-1-activation-in-nk-cells-and-their-subsets-influence-of-receptors-maturation-and-cytokine-stimulation
#20
Doris Urlaub, Kristine Höfer, Martha-Lena Müller, Carsten Watzl
The integrin LFA-1 is essential for efficient activation and for cytotoxicity of NK cells because it initiates the assembly of the immunological synapse and mediates firm adhesion to the target. LFA-1 is also needed to polarize the cytotoxic machinery of the NK cell toward the target cell. The binding affinity and avidity of integrins can be regulated via inside-out signals from other receptors. In this article, we investigate the signals necessary to activate LFA-1 in human NK cells. Our data show that LFA-1 has a low ligand-binding activity in resting human NK cells, but it can be stimulated by triggering activating receptors, such as 2B4 or CD16, or by coactivation of different receptor combinations...
January 18, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
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