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https://www.readbyqxmd.com/read/29165355/acute-effects-of-nitrate-rich-beetroot-juice-on-blood-pressure-hemostasis-and-vascular-inflammation-markers-in-healthy-older-adults-a-randomized-placebo-controlled-crossover-study
#1
Kyle Raubenheimer, Danica Hickey, Michael Leveritt, Robert Fassett, Joaquin Ortiz de Zevallos Munoz, Jason D Allen, David Briskey, Tony J Parker, Graham Kerr, Jonathan M Peake, Natalie M Pecheniuk, Oliver Neubauer
Aging is associated with a vasoconstrictive, pro-coagulant, and pro-inflammatory profile of arteries and a decline in the bioavailability of the endothelium-derived molecule nitric oxide. Dietary nitrate elicits vasodilatory, anti-coagulant and anti-inflammatory effects in younger individuals, but little is known about whether these benefits are evident in older adults. We investigated the effects of 140 mL of nitrate-rich (HI-NI; containing 12.9 mmol nitrate) versus nitrate-depleted beetroot juice (LO-NI; containing ≤0...
November 22, 2017: Nutrients
https://www.readbyqxmd.com/read/29163501/herceptin-enhances-the-antitumor-effect-of-natural-killer-cells-on-breast-cancer-cells-expressing-human-epidermal-growth-factor-receptor-2
#2
Xiao Tian, Feng Wei, Limei Wang, Wenwen Yu, Naining Zhang, Xinwei Zhang, Ying Han, Jinpu Yu, Xiubao Ren
Optimal adoptive cell therapy (ACT) should contribute to effective cancer treatment. The unique ability of natural killer (NK) cells to kill cancer cells independent of major histocompatibility requirement makes them suitable as ACT tools. Herceptin, an antihuman epidermal growth factor receptor-2 (anti-HER2) monoclonal antibody, is used to treat HER2(+) breast cancer. However, it has limited effectiveness and possible severe cardiotoxicity. Given that Herceptin may increase the cytotoxicity of lymphocytes, we explored the possible augmentation of NK cell cytotoxicity against HER2(+) breast cancer cells by Herceptin...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29154680/cardiovascular-disease-predictors-and-adipose-tissue-macrophage-polarization-is-there-a-link
#3
Ivana Kralova Lesna, Marek Petras, Sona Cejkova, Anna Kralova, Jiri Fronek, Libor Janousek, Filip Thieme, Tomas Tyll, Rudolf Poledne
Background The risk of cardiovascular disease is closely connected to adipose tissue inflammation. The links between cardiovascular risk predictors and pro and anti-inflammatory macrophages in human adipose tissue were analysed to gain an insight into the pathophysiology of cardiovascular disease. Design Subcutaneous and visceral adipose tissues were obtained from 79 subjects, 52 living kidney donors (during nephrectomy) and 27 patients with peripheral artery disease (during arterial tree reconstruction). Methods Macrophage subsets were isolated from adipose tissues and analysed by flow cytometry using CD14, CD16, CD36 and CD163 monoclonal antibodies...
January 1, 2017: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/29142617/acute-strenuous-exercise-induces-an-imbalance-on-histone-h4-acetylation-histone-deacetylase-2-and-increases-the-proinflammatory-profile-of-pbmc-of-obese-individuals
#4
Gilson P Dorneles, Maria Carolina R Boeira, Lucas L Schipper, Ivy R V Silva, Viviane R Elsner, Pedro Dal Lago, Alessandra Peres, Pedro R T Romão
This study evaluated the response of global histone H4 acetylation (H4ac), histone deacetylase 2 (HDAC2) activity, as well as the production of proinflammatory cytokines and monocyte phenotypes of lean and obese males after exercise. Ten lean and ten obese sedentary men were submitted to one session of strenuous exercise, and peripheral blood mononuclear cells (PBMC) were stimulated in vitro with lipopolysaccharide (LPS). Global H4ac levels, HDAC2 activity in PBMC, and IL-6, IL-8, and TNF-α production were analyzed...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29141868/geographic-clonal-tracking-in-macaques-provides-insights-into-hspc-migration-and-differentiation
#5
Chuanfeng Wu, Diego A Espinoza, Samson J Koelle, E Lake Potter, Rong Lu, Brian Li, Di Yang, Xing Fan, Robert E Donahue, Mario Roederer, Cynthia E Dunbar
The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesus macaque autologous HSPCs with genetic barcodes, allowing clonal tracking over time and space after transplantation. We found marked geographic segregation of CD34(+) HSPCs for at least 6 mo posttransplantation, followed by very gradual clonal mixing at different BM sites over subsequent months to years...
November 15, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29137628/hypertonic-saline-for-prevention-of-delirium-in-geriatric-patients-who-underwent-hip-surgery
#6
Xi Xin, Fei Xin, Xuguang Chen, Qi Zhang, Yanan Li, Shuping Huo, Chongfu Chang, Qiujun Wang
BACKGROUND: Postoperative delirium (POD) is a common disorder in the elderly patients, and neuroinflammation is the possible underlying mechanism. This study is designed to determine whether or not hypertonic saline (HS) pre-injection can alleviate POD in aged patients. METHODS: This prospective study recruited 120 geriatric patients who underwent hip surgery. The patients were randomly divided into two groups: control group (NS group) and HS group. Patients in the NS group were pre-injected with 4 mL/kg isotonic saline, and those in the HS group were pre-injected with 4 mL/kg 7...
November 14, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29133194/effect-of-native-and-minimally-modified-low-density-lipoprotein-on-the-activation-of-monocyte-subsets
#7
Francisco Blanco-Favela, José Esteban Espinosa-Luna, Adriana Karina Chávez-Rueda, Alejandra Madrid-Miller, Luis Chávez-Sánchez
BACKGROUND: In atherosclerosis, monocytes are essential and secrete pro-inflammatory cytokines in response to modified low-density lipoprotein (LDL). Human CD14(++)CD16(-), CD14(++)CD16(+) and CD14(+)CD16(++) monocytes produce different cytokines. The objective of this research was to determine the number of monocyte subsets positives to cytokines in response to native (nLDL) and minimally modified LDL (mmLDL). METHODS: Human monocytes from healthy individuals were purified by negative selection and were stimulated with nLDL, mmLDL or LPS...
November 10, 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/29130617/dendritic-cell-phenotype-in-severe-asthma-reflects-clinical-responsiveness-to-glucocorticoids
#8
Emma S Chambers, Alexandra M Nanzer, Paul E Pfeffer, David F Richards, Adrian R Martineau, Christopher J Griffiths, Christopher J Corrigan, Catherine M Hawrylowicz
BACKGROUND: Subsets of patients with severe asthma remain symptomatic despite prolonged, high dosage glucocorticoid therapy. We hypothesised that the clinical glucocorticoid sensitivity of these asthmatics is reflected in differences in peripheral blood dendritic cell subsets. OBJECTIVE: To compare peripheral blood leukocyte populations using flow cytometry at baseline and after 2 weeks of systemic glucocorticoid (steroid) treatment to identify immunological differences between steroid sensitive (SS) and steroid resistant (SR) asthmatics...
November 11, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29128554/immunomodulatory-effects-of-tyrosine-kinase-inhibitors-tkis-in-vitro-and-in-vivo-study
#9
Elena Marinelli Busilacchi, Andrea Costantini, Nadia Viola, Benedetta Costantini, Jacopo Olivieri, Luca Butini, Giorgia Mancini, Ilaria Scortechini, Martina Chiarucci, Monica Poiani, Antonella Poloni, Pietro Leoni, Attilio Olivieri
Pathogenesis of chronic Graft Versus Host Disease (cGVHD) is incompletely defined, involving donor-derived CD4 and CD8-positive T lymphocytes, as well as B cells. Standard treatment is lacking for steroid dependent/refractory cases; potential usefulness of tyrosine kinase inhibitors (TKIs) has been suggested, based on their potent anti-fibrotic effect. However, TKI seems to have pleiotropic activity. We sought to evaluate the in vitro and in vivo impact of different TKIs on lymphocyte phenotype and function...
November 8, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29128509/monocyte-activation-is-elevated-in-women-with-knee-osteoarthritis-and-associated-with-inflammation-bmi-and-pain
#10
D Loukov, S Karampatos, M R Maly, D M E Bowdish
OBJECTIVE: Monocytes contribute to synovitis and disease pathogenesis in osteoarthritis (OA). Low-grade inflammation occurs in OA and correlates with disease severity and progression. Since monocyte development and function is altered by systemic inflammation, we analyzed monocyte numbers and function between individuals with knee OA and healthy age- and sex-matched controls. DESIGN: We analyzed markers of soluble and cellular inflammation in peripheral blood of women with knee OA and compared them to healthy age- and sex-matched controls...
November 8, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29123530/human-cardiac-derived-stem-progenitor-cells-fine-tune-monocyte-derived-descendants-activities-toward-cardiac-repair
#11
Noémie Dam, Hocine Rachid Hocine, Itziar Palacios, Olga DelaRosa, Ramón Menta, Dominique Charron, Armand Bensussan, Hicham El Costa, Nabila Jabrane-Ferrat, Wilfried Dalemans, Eleuterio Lombardo, Reem Al-Daccak
Cardiac repair following MI relies on a finely regulated immune response involving sequential recruitment of monocytes to the injured tissue. Monocyte-derived cells are also critical for tissue homeostasis and healing process. Our previous findings demonstrated the interaction of T and natural killer cells with allogeneic human cardiac-derived stem/progenitor cells (hCPC) and suggested their beneficial effect in the context of cardiac repair. Therefore, we investigated here whether monocytes and their descendants could be also modulated by allogeneic hCPC toward a repair/anti-inflammatory phenotype...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29117436/isolation-cryopreservation-and-immunophenotyping-of-human-peripheral-blood-mononuclear-cells
#12
Fredine T Lauer, Jesse L Denson, Scott W Burchiel
This unit describes procedures for the isolation, cryopreservation, and thawing of human peripheral blood mononuclear cells (HPBMC) and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This methodology can be used to ensure that cell integrity and phenotype stability are not altered through cryopreservation and extended storage. For this analysis, HPBMC were isolated from 7 healthy individuals, and 11-color flow cytometry was performed on freshly isolated samples as well as samples cryopreserved for short- and long-term periods...
November 8, 2017: Current Protocols in Toxicology
https://www.readbyqxmd.com/read/29116124/monocytes-from-patients-with-primary-ciliary-dyskinesia-show-enhanced-inflammatory-properties-and-produce-higher-levels-of-pro-inflammatory-cytokines
#13
M Cockx, M Gouwy, P Ruytinx, I Lodewijckx, A Van Hout, S Knoops, N Pörtner, I Ronsse, L Vanbrabant, V Godding, K De Boeck, J Van Damme, M Boon, S Struyf
Patients with Primary Ciliary Dyskinesia (PCD) suffer from recurrent upper and lower airway infections due to defects in the cilia present on the respiratory epithelium. Since chronic inflammatory conditions can cause changes in innate immune responses, we investigated whether monocytes isolated from the peripheral blood of pediatric PCD patients respond differently to inflammatory stimuli, compared to monocytes from healthy children and adults. The receptor for C5a (C5aR) was upregulated in PCD, whereas expression levels of the leukocyte chemoattractant receptors CCR1, CCR2, CCR5, BLT1 and FPR1 on PCD monocytes were similar to those on monocytes from healthy individuals...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29112724/severe-influenza-is-characterized-by-prolonged-immune-activation-results-from-the-shivers-cohort-study
#14
Sook-San Wong, Christine M Oshansky, Xi-Zhi J Guo, Jacqui Ralston, Timothy Wood, Gary Reynolds, Ruth Seeds, Claire Newbern, Ben Waite, Marc-Alain Widdowson, Q Sue Huang, Richard J Webby, Paul G Thomas
Background: The immunologic factors underlying severe influenza are poorly understood. To address this, we compared the immune responses of influenza-confirmed hospitalized individuals with severe acute respiratory illness (SARI) to those of non-hospitalized individuals with influenza-like illness (ILI). Methods: Peripheral blood lymphocytes were collected from ILI (N=27) and SARI-patients (N=27) at time of enrollment and then two weeks later. Innate and adaptive cellular immune responses were assessed by flow-cytometry and serum cytokines were assessed by bead-based assay...
November 3, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29112629/maximal-exercise-alters-the-inflammatory-phenotype-and-response-of-mononuclear-cells
#15
Aaron L Slusher, Tiffany M Zúñiga, Edmund O Acevedo
PURPOSE: Monocytes express the CD14 receptor that facilitates lipopolysaccharide (LPS) ligation to toll-like receptor 4 (TLR4) to elicit production of interleukin (IL)-6, IL-10, and tumor necrosis factor alpha (TNF-α). However, pro-inflammatory conditions, such as strenuous exercise, increase the percentage of monocytes expressing CD16, a receptor that enhances LPS stimulated TNF-α production. Therefore, we examined whether maximal treadmill exercise would alter the inflammatory phenotype of classical (CD14/CD16) and pro-inflammatory monocytes (intermediate [CD14/CD16] and non-classical [CD14/CD16]), evidenced by changes in TLR4, CD14, and CD16 receptor expression, and their inflammatory response to ex vivo LPS stimulation...
November 6, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29112187/number-of-circulating-non-classical-cd14-cd16-monocytes-negatively-correlates-with-das28-and-swollen-joints-count-in-peripheral-spondyloarthritis-patients
#16
Zofia Guła, Małgorzata Stec, Magdalena Rutkowska-Zapała, Marzena Lenart, Mariusz Korkosz, Jerzy Gąsowski, Jarosław Baran, Monika Baj-Krzyworzeka, Rafał Szatanek, Jarosław Czyż, Maciej Siedlar
INTRODUCTION    Different clinical course and pattern of skeletal involvement in peripheral (per) and axial (ax) spondyloarthritis (SpA) suggests distinct pathophysiology of these two phenotypic manifestations of SpA. Monocytes, as a part of innate immune system, seem to play an important role in the pathogenesis of SpA, but exact inflammatory pathways remain to be elucidated. Regulatory T lymphocytes (Treg) and Th17 lymphocytes are also known to influence pro- and anti-inflammatory reactions. OBJECTIVES    The aim of our study was to compare absolute numbers of monocyte subpopulations, Treg and Th17 lymphocytes with clinical measures of disease activity in perSpA and axSpA...
November 7, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/29111217/ruxolitinib-partially-reverses-functional-nk-cell-deficiency-in-patients-with-stat1-gain-of-function-mutations
#17
Alexander Vargas-Hernandez, Emily M Mace, Ofer Zimmerman, Christa S Zerbe, Alexandra F Freeman, Sergio Rosenzweig, Jennifer W Leiding, Troy Torgerson, Matthew C Altman, Edith Schussler, Charlotte Cunningham-Rundles, Ivan K Chinn, Alexandre F Carisey, Imelda C Hanson, Nicholas L Rider, Steven M Holland, Jordan S Orange, Lisa R Forbes
BACKGROUND: Natural Killer (NK) cells are critical innate effector cells whose development is dependent on the JAK-STAT pathway. NK deficiency can result in severe or refractory viral infections. Patients with Signal Transducer and Activator of Transcription (STAT)1 gain of function (GOF) mutations have increased viral susceptibility. OBJECTIVE: We sought to investigate NK cell function in STAT1 GOF patients. METHODS: NK cell phenotype and function were determined in 16 STAT1 GOF patients...
October 27, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29109719/phenotypic-characterization-of-human-monocytes-following-macronutrient-intake-in-healthy-humans
#18
Awad Alshahrani, Abdalmalik Bin Khunayfir, Mohammed Al Rayih, Hasan Al Sayed, Abdullah Alsadoon, Mohammed Al Dubayee, Mahmoud Zahra, Yousof Alrumayyan, Maha Al Zayer, Amre Nasr, Ahmad Aljada
Background: Three subsets of human monocytes in circulation have been identified and their characterization is still ill-defined. Although glucose and lipid intakes have been demonstrated to exert pro-inflammatory effects on mononuclear cells (MNCs) of healthy subjects, characterization of monocytes phenotypes following macronutrient (glucose, protein, and lipid) intake in humans remains to be determined. Methods: Thirty-six healthy, normal weight volunteers were recruited in the study...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29109620/erratum-stigma-in-people-with-type-1-or-type-2-diabetes-clinical-diabetes-2017-35-27-34-doi-10-2337-cd16-0020
#19
Nancy F Liu, Adam S Brown, Michael F Younge, Susan J Guzman, Kelly L Close, Richard Wood
[This corrects the article on p. 27 in vol. 35, PMID: 28144043.].
October 2017: Clinical Diabetes: a Publication of the American Diabetes Association
https://www.readbyqxmd.com/read/29108126/high-sensitivity-of-the-hematoflow%C3%A2-solution-for-chronic-myelomonocytic-leukemia-screening
#20
Romain Vazquez, Mikael Roussel, Bouchra Badaoui, Nicolas Freynet, Sihem Tarfi, Eric Solary, Dorothée Selimoglu-Buet, Orianne Wagner-Ballon
BACKGROUND: Accumulation of classical monocytes CD14(++) CD16(-) (also called MO1) ≥94% can accurately distinguish chronic myelomonocytic leukemia (CMML) from reactive monocytosis. The HematoFlow™ solution, able to quantify CD16 negative monocytes, could be a useful tool to manage monocytosis which remains a common issue in routine laboratories. METHODS: Classical monocytes were quantified from 153 whole blood samples collected on EDTA using both flow cytometry methods, either MO1 percentage determination by the multiparameter assay previously published and regarded here as the reference method, or CD16 negative monocyte percentage determination by the means of HematoFlow™...
November 6, 2017: Cytometry. Part B, Clinical Cytometry
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