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intraperitoneal candidiasis

Alexander J Lepak, Miao Zhao, Brian VanScoy, Paul G Ambrose, David R Andes
Echinocandins are important in the prevention and treatment of invasive candidiasis but limited by current dosing regimens that include daily intravenous administration. The novel echinocandin CD101 has a prolonged half-life of approximately 130 h in humans, making it possible to design once-weekly dosing strategies. The present study examined the pharmacodynamic activity of CD101 using the neutropenic invasive candidiasis mouse model against select Candida albicans ( n = 4), C. glabrata ( n = 3), and C. parapsilosis ( n = 3) strains...
February 2018: Antimicrobial Agents and Chemotherapy
Nathan P Wiederhold, Laura K Najvar, Rosie Jaramillo, Marcos Olivo, Jason Pizzini, Gabriel Catano, Thomas F Patterson
Background: Echinocandins are recommended as first-line therapy against Candida glabrata infections, although increased resistance to this class has been reported worldwide and they are currently only available for parenteral administration. SCY-078 is an investigational glucan synthase inhibitor that is orally available. Objectives: To evaluate the in vivo efficacy of SCY-078 in an experimental model of invasive candidiasis due to WT and echinocandin-resistant C...
February 1, 2018: Journal of Antimicrobial Chemotherapy
Katalin Csonka, Máté Vadovics, Annamária Marton, Csaba Vágvölgyi, Erik Zajta, Adél Tóth, Renáta Tóth, Csaba Vizler, László Tiszlavicz, Héctor M Mora-Montes, Attila Gácser
Candida parapsilosis is an opportunistic human fungal pathogen that poses a serious threat to low birth weight neonates, particularly at intensive care units. In premature infants, the distinct immune responses to Candida infections are not well understood. Although several in vivo models exist to study systemic candidiasis, only a few are available to investigate dissemination in newborns. In addition, the majority of related studies apply intraperitoneal infection rather than intravenous inoculation of murine infants that may be less efficient when studying systemic invasion...
2017: Frontiers in Microbiology
Samir Jawhara, Elzbieta Pluskota, Wei Cao, Edward F Plow, Dmitry A Soloviev
Integrins αMβ2 and αXβ2 are homologous adhesive receptors that are expressed on many of the same leukocyte populations and bind many of the same ligands. Although αMβ2 was extensively characterized and implicated in leukocyte inflammatory and immune functions, the roles of αXβ2 remain largely obscure. Here, we tested the ability of mice deficient in integrin αMβ2 or αXβ2 to deal with opportunistic infections and the capacity of cells derived from these animals to execute inflammatory functions. The absence of αMβ2 affected the recruitment of polymorphonuclear neutrophils (PMN) to bacterial and fungal pathogens as well as to model inflammatory stimuli, and αMβ2-deficient PMN displayed defective inflammatory functions...
January 2017: Infection and Immunity
Fabíola Maria Marques do Couto, Silene Carneiro do Nascimento, Silvio Francisco Pereira Júnior, Vanessa Karina Alves da Silva, André Ferraz Goiana Leal, Rejane Pereira Neves
This work aimed to evaluate the antifungal activity of piroctone olamine in the treatment of intra-abdominal candidiasis in an experimental model using Swiss mice. The mice (n = 6) were infected by intraperitoneal injection of 0.2 ml of C. albicans (10(7)cells/ml in saline). The animals were observed daily for clinical signs and mortality for 14 days. The treatment with piroctone olamine (0.5 mg/kg) was performed 72 h after infection by intraperitoneal administration. For comparison, a group of animals (n = 6) was treated with amphotericin B (0...
2016: SpringerPlus
Nathan P Wiederhold, Laura K Najvar, Annette W Fothergill, Rosie Bocanegra, Marcos Olivo, Dora I McCarthy, Yoshiko Fukuda, Junichi Mitsuyama, Thomas F Patterson
OBJECTIVES: Candida species are major causes of invasive mycoses in immunocompetent and immunocompromised hosts. Treatment options are limited in the setting of antifungal resistance and increased rates of echinocandin-resistant Candida glabrata have been reported. The novel arylamidine T-2307 demonstrates potent in vitro antifungal activity against Candida species. Our objective was to evaluate the in vitro and in vivo activity of T-2307 against resistant C. glabrata. METHODS: In vitro activity was determined against 42 clinical C...
March 2016: Journal of Antimicrobial Chemotherapy
Sofia Maraki, George Hamilos, Dimitra Dimopoulou, Angeliki M Andrianaki, Alexander Steven Karageorgiadis, Andreas Kyvernitakis, Stelios Lionakis, Diamantis P Kofteridis, George Samonis
Colonization of the gastrointestinal (GI) tract by Candida species is a principal pathogenetic event for development of invasive candidiasis. Importantly, the effect of echinocandins, the preferred antifungal agents for treatment of invasive candidiasis, on GI tract colonization by Candida spp. is currently unknown. Herein, we used an established model of persistent murine GI tract colonization by Candida albicans to test the ability of different echinocandins to eradicate the yeast from murine gut. Adult male Crl:CD1 (ICR) BR mice were fed with chow containing C...
August 2015: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Carlos López-Abarrategui, Christine McBeth, Santi M Mandal, Zhenyu J Sun, Gregory Heffron, Annia Alba-Menéndez, Ludovico Migliolo, Osvaldo Reyes-Acosta, Mónica García-Villarino, Diego O Nolasco, Rosana Falcão, Mariana D Cherobim, Simoni C Dias, Wolfgang Brandt, Ludger Wessjohann, Michael Starnbach, Octavio L Franco, Anselmo J Otero-González
Antimicrobial peptides form part of the first line of defense against pathogens for many organisms. Current treatments for fungal infections are limited by drug toxicity and pathogen resistance. Cm-p5 (SRSELIVHQRLF), a peptide derived from the marine mollusk Cenchritis muricatus peptide Cm-p1, has a significantly increased fungistatic activity against pathogenic Candida albicans (minimal inhibitory concentration, 10 µg/ml; EC50, 1.146 µg/ml) while exhibiting low toxic effects against a cultured mammalian cell line...
August 2015: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Gunlawadee Maneenil, Matthew S Payne, Paranthaman Senthamarai Kannan, Suhas G Kallapur, Boris W Kramer, John P Newnham, Yuichiro Miura, Alan H Jobe, Matthew W Kemp
BACKGROUND: Intrauterine Candida albicans infection causes severe fetal inflammatory responses and fetal injury in an ovine model. We hypothesized that intra-amniotic antifungal therapy with fluconazole would decrease the adverse fetal effects of intra-amniotic C. albicans in sheep. METHODS: Sheep received an intra-amniotic injection of 10(7) colony-forming units C. albicans. After 2 d, animals were then randomized to: (i) intra-amniotic and fetal intraperitoneal saline with delivery after 24 h (3 d C...
June 2015: Pediatric Research
Paulo Vítor Soeiro-Pereira, Angela Falcai, Christina Arslanian Kubo, Edson Antunes, Antonio Condino-Neto
In our previous study, we have found that 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]-pyrimidin-4-ylamine (BAY 41-2272), a guanylate cyclase agonist, activates human monocytes and the THP-1 cell line to produce the superoxide anion, increasing in vitro microbicidal activity, suggesting that this drug can be used to modulate immune functioning in primary immunodeficiency patients. In the present work, we investigated the potential of the in vivo administration of BAY 41-2272 for the treatment of Candida albicans and Staphylococcus aureus infections introduced via intraperitoneal and subcutaneous inoculation...
February 2015: Memórias do Instituto Oswaldo Cruz
Evelyn E Nash, Brian M Peters, Glen E Palmer, Paul L Fidel, Mairi C Noverr
Intra-abdominal polymicrobial infections cause significant morbidity and mortality. An established experimental mouse model of Staphylococcus aureus-Candida albicans intra-abdominal infection results in ∼60% mortality within 48 h postinoculation, concomitant with amplified local inflammatory responses, while monomicrobial infections are avirulent. The purpose of this study was to characterize early local and systemic innate responses during coinfection and determine the role of C. albicans morphogenesis in lethality, a trait involved in virulence and physical interaction with S...
August 2014: Infection and Immunity
Shaoji Cheng, Cornelius J Clancy, Douglas J Hartman, Binghua Hao, M Hong Nguyen
The pathogenesis of Candida glabrata infections is poorly understood. We studied the pathogenesis of intra-abdominal candidiasis (IAC) in mice that were infected intraperitoneally with C. glabrata and sterile feces. C. glabrata BG2 (5 × 10(8) CFU) caused a 100% mortality rate. Sublethal inocula of BG2 (1 × 10(8) or 1 × 10(7) CFU) caused peritonitis that progressed to abscesses. Three clinical C. glabrata strains (5 × 10(8) CFU) caused 80 to 100% mortality rates, while a fourth (strain 346) caused a 29% mortality rate...
July 2014: Infection and Immunity
Aurélie Crabbé, Sheila M Nielsen-Preiss, Christine M Woolley, Jennifer Barrila, Kent Buchanan, James McCracken, Diane O Inglis, Stephen C Searles, Mayra A Nelman-Gonzalez, C Mark Ott, James W Wilson, Duane L Pierson, Heidemarie M Stefanyshyn-Piper, Linda E Hyman, Cheryl A Nickerson
This study presents the first global transcriptional profiling and phenotypic characterization of the major human opportunistic fungal pathogen, Candida albicans, grown in spaceflight conditions. Microarray analysis revealed that C. albicans subjected to short-term spaceflight culture differentially regulated 452 genes compared to synchronous ground controls, which represented 8.3% of the analyzed ORFs. Spaceflight-cultured C. albicans-induced genes involved in cell aggregation (similar to flocculation), which was validated by microscopic and flow cytometry analysis...
2013: PloS One
A R Khosravi, H Shokri, Z Tootian, M Alizadeh, R Yahyaraeyat
Disseminated candidiasis is a serious problem in public health that results from the invasion of Candida species, in particular Candida albicans. The aim of this study was to compare the efficacies of Zataria multiflora essential oil and itraconazole in clearing C. albicans from the visceral organs of BALB/c mice suffered from disseminated candidiasis. Zataria multiflora essential oil was extracted using Clevenger-type apparatus and analyzed by gas chromatography mass spectrometry (GC-MS). For clearance experiment, mice (20-25 g, N=8 per group) received essential oil at doses of 30, 48 and 64 mg/kg and itraconazole at dose of 200 mg/kg intraperitoneally (IP) 2 days before and after intravenous inoculation of 0...
July 2009: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Shaoji Cheng, Cornelius J Clancy, Wenjie Xu, Frank Schneider, Binghua Hao, Aaron P Mitchell, M Hong Nguyen
BACKGROUND: The pathogenesis of intra-abdominal candidiasis is poorly understood. METHODS: Mice were intraperitoneally infected with Candida albicans (1 × 10(6) colony-forming units) and sterile stool. nanoString assays were used to quantitate messenger RNA for 145 C. albicans genes within the peritoneal cavity at 48 hours. RESULTS: Within 6 hours after infection, mice developed peritonitis, characterized by high yeast burdens, neutrophil influx, and a pH of 7...
November 1, 2013: Journal of Infectious Diseases
Jeonghyeon Kim, Inkyung Joo, Hayan Kim, Yongmoon Han
The aim of this study was to determine the immunological adjuvant effect of 18β-glycyrrhetinic acid (GA) isolated from Glycyrrhizae radix. In the experiments, BALB/c mice were immunized on days 1 and 22 intraperitoneally (i.p.) with an emulsion form of Candida albicans surface mannan extract (SM) mixed with either Incomplete Freund's Adjuvant [SM/IFA], or Complete Freund's Adjuvant [SM/CFA] or GA mixed with IFA [SM/GA/IFA]. One week after the second immunization, polyclonal sera were collected from these animals in order to determine IgG isotypes and cytokine profiles in the sera...
August 15, 2013: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Rong Tian, Meng Fu, Zhuo Zhang, Jing Ren, Jingang An, Yufeng Liu, Wei Li
B-1 cells are innate-like cells that play important roles in host defense against infection. However, the function of B-1 cells in fungi infection remains unclear. Previously we produced IgH transgenic mice TgVH3B4 with VH derived from a natural antibody 3B4 that can identify C. albicans, and found that TgVH3B4 mice were resistant to intraperitoneal (i. p.) and intravenous C. albicans infection. Most of the peritoneal cavity (PEC) B-1 cells in TgVH3B4 mice express transgenic BCR that binds C. albicans. In the present study, we explored the response of B-1 cells to C...
2013: PloS One
Jeonghyeon Kim, Britta J Han, Hayan Kim, Ju Young Lee, Inkyoung Joo, Shezad Omer, Yeong Shik Kim, Yongmoon Han
Type-1 and -2 responses of T helper lymphocytes demonstrate essentially different and opposite effector functions. In the present study, we determined the immunoregulatory effect of ginsenoside Re against disseminated candidiasis due to Candida albicans. This fungus may be one of the most problematic fungi for humans. Results showed that Re had no growth-inhibitory effect on C. albicans. In contrast, mice groups given Re intraperitoneally before intravenous challenge with live C. albicans survived longer against disseminated candidiasis than Re-untreated mice...
December 2012: International Immunopharmacology
Gabriela Marranzino, Julio Villena, Susana Salva, Susana Alvarez
Lactobacillus rhamnosus CRL1505 (Lr1505), L. rhamnosus CRL1506 (Lr1506) and L. casei CRL431 (Lc431) are able to stimulate intestinal immunity, but only Lr1505 and Lc431 are able to stimulate immunity in the respiratory tract. With the aim of advancing the understanding of the immunological mechanisms involved in stimulation of distant mucosal sites, this study evaluated the effects of orally administered probiotics on the functions of alveolar and peritoneal macrophages. Compared to a control group, these three lactobacilli were able to significantly increase phagocytic and microbicidal activities of peritoneal macrophages...
November 2012: Microbiology and Immunology
J Szilágyi, R Földi, R Gesztelyi, S Bayegan, G Kardos, B Juhász, L Majoros
BACKGROUND: The efficacy of fluconazole (FLU), amphotericin B (AMB) and caspofungin (CAS) was tested against three Candida orthopsilosis, three C. metapsilosis and two C. parapsilosis sensu stricto isolates in neutropenic mice. METHODS: Mice were immunosuppressed by 200 mg/kg cyclophosphamide. Five-day intraperitoneal treatment was started 24 h after infection. Kidney burden was analyzed using the Kruskal-Wallis test. RESULTS: FLU 10 and 20 mg/kg as well as AMB 1 mg/kg significantly decreased the fungal burden (p < 0...
2012: Chemotherapy
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