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https://www.readbyqxmd.com/read/29782690/stable-carboxylate-terminated-gold-surfaces-produced-by-spontaneous-grafting-of-an-alkyl-tin-compound
#1
Mayreli Ortiz, Ahmed Mehdi, Christophe Methivier, Serge Thorimbert, Bernold Hasenknopf, Ciara K O'Sullivan
Self-assembled monolayers formed by chemisorption of thiolated molecules on gold surfaces are widely applied for biosensing. Moreover, and due to the low stability of thiol-gold chemistry, contributions to the functionalisation of gold substrates with linkers that provide a more stable platform for the immobilisation of electroactive or biological molecules are highly appreciated. In the work reported here, we demonstrate that a carboxylated organotin compound can be successfully grafted onto gold substrates to form a highly stable organic layer with reactivity for subsequent binding to an aminated molecule...
May 21, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29780479/pentacyclic-adenine-a-versatile-and-exceptionally-bright-fluorescent-dna-base-analogue
#2
Mattias Bood, Anders F Füchtbauer, Moa S Wranne, Jong Jin Ro, Sangamesh Sarangamath, Afaf H El-Sagheer, Déborah L M Rupert, Rachel S Fisher, Steven W Magennis, Anita C Jones, Fredrik Höök, Tom Brown, Byeang Hyean Kim, Anders Dahlén, L Marcus Wilhelmsson, Morten Grøtli
Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties. When incorporated into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far. Together with the recently established base analog acceptor qAnitro , pA allows accurate distance and orientation determination via Förster resonance energy transfer (FRET) measurements...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29777767/biglycan-is-a-new-high-affinity-ligand-for-cd14-in-macrophages
#3
Heiko Roedig, Madalina V Nastase, Helena Frey, Kristin Moreth, Jinyang Zeng-Brouwers, Chiara Poluzzi, Louise Tzung-Harn Hsieh, Christian Brandts, Simone Fulda, Malgorzata Wygrecka, Liliana Schaefer
Sterile inflammation is a therapeutic target in many diseases where it represents an important initiator of disease progression. However, the detailed mechanisms underlying its evolution and biological relevance are not yet completely elucidated. Biglycan, a prototype extracellular matrix-derived damage-associated molecular pattern, mediates sterile inflammation in macrophages through Toll-like receptor (TLR) 2 and/or TLR4-dependent signaling pathways. Here we discovered that soluble biglycan is a novel high-affinity ligand for CD14, a well-known GPI-anchored co-receptor for TLRs...
May 16, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29775278/hybrid-nanomaterials-with-single-site-catalysts-by-spatially-controllable-immobilization-of-nickel-complexes-via-photoclick-chemistry-for-alkene-epoxidation
#4
Dwaipayan Ghosh, Benny Febriansyah, Disha Gupta, Leonard Kia-Sheun Ng, Shibo Xi, Yonghua Du, Tom Baikie, ZhiLi Dong, Han Sen Soo
Catalyst deactivation is a persistent problem not only for the scientific community but also in industry. Isolated single-site heterogeneous catalysts have shown great promise to overcome these problems. Here, a versatile anchoring strategy for molecular complex immobilization on a broad range of semiconducting or insulating metal oxide (e.g. titanium dioxide, mesoporous silica, cerium oxide, and tungsten oxide) nanoparticles to synthesize isolated single-site catalysts has been studied systematically. An oxidatively stable anchoring group, maleimide, is shown to form covalent linkages with surface hydroxyl functionalities of metal oxide nanoparticles by photoclick chemistry...
May 18, 2018: ACS Nano
https://www.readbyqxmd.com/read/29772810/determination-of-the-membrane-environment-of-cd59-in-living-cells
#5
Gergő Fülöp, Mario Brameshuber, Andreas M Arnold, Gerhard J Schütz, Eva Sevcsik
The organization and dynamics of proteins and lipids in the plasma membrane, and their role in membrane functionality, have been subject of a long-lasting debate. Specifically, it is unclear to what extent membrane proteins are affected by their immediate lipid environment and vice versa. Studies on model membranes and plasma membrane vesicles indicated preferences of proteins for lipid phases characterized by different acyl chain order; however, whether such phases do indeed exist in live cells is still not known...
May 17, 2018: Biomolecules
https://www.readbyqxmd.com/read/29772751/what-we-do-not-know-about-fungal-cell-adhesion-molecules
#6
REVIEW
Peter N Lipke
There has been extensive research on structure and function of fungal cell adhesion molecules, but the most of the work has been about adhesins in Candida albicans and Saccharomyces cerevisiae. These yeasts are members of a single ascomycete order, and adhesion molecules from the six other fungal phyla are only sparsely described in the literature. In these other phyla, most of the research is at the cellular level, rather than at the molecular level, so there has been little characterization of the adhesion molecules themselves...
May 17, 2018: Journal of Fungi (Basel, Switzerland)
https://www.readbyqxmd.com/read/29772403/theoretical-study-of-the-intermolecular-recognition-mechanism-between-survivin-and-substrate-based-on-conserved-binding-mode-analysis
#7
Chao Wu, Liguang Mao, Xie Huang, Weitao Fu, Xiong Cai, Yuepiao Cai, Liqun Shen, Xiaoxia Ye
Survivin is the smallest member of IAP (inhibitor of apoptosis protein) family, which plays important roles in both mitosis and apoptosis. It has become an attractive drug target due to its overexpression in many human cancers. Survivin has been proven to bind to Smac/DIABLO protein that indirectly inhibits apoptosis. Meanwhile, it is the key subunit of chromosome passenger complex (CPC) which bind to the N-terminal tail of phosphorylated histone H3T3ph during mitosis. Up to now, Survivin directly targeting inhibitor has yet to merge since the difficulty of disrupting the protein-protein interactions (PPIs) between Survivin and its substrate proteins...
May 8, 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29771498/structure-and-function-of-the-transmembrane-domain-of-nsas-an-antibiotic-sensing-histidine-kinase-in-s-aureus
#8
Manasi P Bhate, Thomas Lemmin, Georg Kuenze, Bruk Mensa, Soumya Ganguly, Jason Peters, Nathan Schmidt, Jeffrey G Pelton, Carol Gross, Jens Meiler, William F DeGrado
NsaS is one of four intramembrane histidine kinases (HKs) in Staphylococcus aureus that mediate the pathogen's response to membrane active antimicrobials and human innate immunity. We describe the first integrative structural study of NsaS using a combination of solution state NMR spectroscopy, chemical-crosslinking, molecular modeling and dynamics. Three key structural features emerge: First, NsaS has a short N-terminal amphiphilic helix that anchors its transmembrane (TM) bundle into the inner leaflet of the membrane such that it might sense neighboring proteins or membrane deformations...
May 17, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29770773/highly-ordered-molecular-rotor-matrix-on-a-nanopatterned-template-titanyl-phthalocyanine-molecules-on-feo-pt-111
#9
Shuangzan Lu, Min Huang, Zhihui Qin, Yinghui Yu, Qinmin Guo, Gengyu Cao
Molecular rotors, motors and gears plays important roles in artificial molecular machines, in which rotor and motor matrices are much desirable for large scale bottom-up fabrication of molecular machines. Here we demonstrate the fabrication of a highly ordered molecular rotor matrix by depositing nonplanar dipolar titanyl phthalocyanine (TiOPc, C<sub>32</sub>H<sub>16</sub>N<sub>8</sub>OTi) molecules on Moiré patterned dipolar FeO/Pt(111) substrate...
May 17, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29765659/self-assembly-behaviours-of-peptide-drug-conjugates-influence-of-multiple-factors-on-aggregate-morphology-and-potential-self-assembly-mechanism
#10
Qin Fan, Yujie Ji, Jingjing Wang, Li Wu, Weidong Li, Rui Chen, Zhipeng Chen
Peptide-drug conjugates (PDCs) as self-assembly prodrugs have the unique and specific features to build one-component nanomedicines. Supramolecular structure based on PDCs could form various morphologies ranging from nanotube, nanofibre, nanobelt to hydrogel. However, the assembly process of PDCs is too complex to predict or control. Herein, we investigated the effects of extrinsic factors on assembly morphology and the possible formation of nanostructures based on PDCs. To this end, we designed a PDC consisting of hydrophobic drug ( S )-ketoprofen (Ket) and valine-glutamic acid dimeric repeats peptide (L-VEVE) to study their assembly behaviour...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29764287/function-of-a-membrane-embedded-domain-evolutionarily-multiplied-in-the-gpi-lipid-anchor-pathway-proteins-pig-b-pig-m-pig-u-pig-w-pig-v-and-pig-z
#11
Birgit Eisenhaber, Swati Sinha, Wing-Cheong Wong, Frank Eisenhaber
Distant homology relationships among proteins with many transmembrane regions (TMs) are difficult to detect as they are clouded by the TMs' hydrophobic compositional bias and mutational divergence in connecting loops. In the case of several GPI lipid anchor biosynthesis pathway components, the hidden evolutionary signal can be revealed with dissectHMMER, a sequence similarity search tool focusing on fold-critical, high complexity sequence segments. We find that a sequence module with 10 TMs in PIG-W, described as acyl transferase, is homologous to PIG-U, a transamidase subunit without characterized molecular function, and to mannosyltransferases PIG-B, PIG-M, PIG-V and PIG-Z...
May 15, 2018: Cell Cycle
https://www.readbyqxmd.com/read/29760236/anti-sigma-factor-ylad-regulates-transcriptional-activity-of-sigma-factor-ylac-and-sporulation-via-manganese-dependent-redox-sensing-molecular-switch-in-bacillus-subtilis
#12
Min-Kyu Kwak, Han-Bong Ryu, Sung-Hyun Song, Jin-Won Lee, Sa-Ouk Kang
YlaD, a membrane-anchored anti-sigma factor of Bacillus subtilis , contains a HX3 CXXC motif that functions as a redox-sensing domain and belongs to one of the zinc-coordinated anti-sigma factor families. Despite previously showing that the YlaC transcription is controlled by YlaD, experimental evidence of how the YlaC-YlaD interaction is affected by active cysteines and/or metal ions is lacking. Here, we showed that the P yla promoter is autoregulated solely by YlaC. Moreover, reduced YlaD contained zinc and iron, while oxidized YlaD did not...
May 14, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29758058/structural-studies-and-molecular-dynamics-simulations-suggest-a-processive-mechanism-of-exolytic-lytic-transglycosylase-from-campylobacter-jejuni
#13
Jagamya Vijayaraghavan, Vijay Kumar, Nikhil P Krishnan, Ross T Kaufhold, Ximin Zeng, Jun Lin, Focco van den Akker
The bacterial soluble lytic transglycosylase (LT) breaks down the peptidoglycan (PG) layer during processes such as cell division. We present here crystal structures of the soluble LT Cj0843 from Campylobacter jejuni with and without bulgecin A inhibitor in the active site. Cj0843 has a doughnut shape similar but not identical to that of E. coli SLT70. The C-terminal catalytic domain is preceded by an L-domain, a large helical U-domain, a flexible linker, and a small N-terminal NU-domain. The flexible linker allows the NU-domain to reach over and complete the circular shape, using residues conserved in the Epsilonproteobacteria LT family...
2018: PloS One
https://www.readbyqxmd.com/read/29756444/immobilization-of-an-amphiphilic-molecular-cobalt-catalyst-on-carbon-black-for-ligand-assisted-water-oxidation
#14
Habib Baydoun, Jordyn Burdick, Bishnu Thapa, Lanka Wickramasinghe, Da Li, Jens Niklas, Oleg G Poluektov, H Bernhard Schlegel, Cláudio N Verani
We have prepared the amphiphilic molecular catalyst [CoIII (LOC18 )(pyrr)2 ]ClO4 (1), where LOC18 is the deprotonated form of N, N'-[4,5-bis(octadecyloxy)-1,2-phenylene]dipicolinamide. Species 1 can be anchored onto a carbon black support to yield the assembly 1@CB, which can catalyze water oxidation at an affordable onset overpotential of 0.32 V, with a current density of 10 mA/cm2 at 0.37 V. Moreover, 1@CB displays TOF = 3850 h-1 . A mechanism is proposed based on the experimental and density-functional-theory-calculated data...
May 14, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29752964/identification-and-characterization-of-a-calmodulin-binding-domain-in-the-plasma-membrane-ca-2-atpase-from-trypanosoma-equiperdum
#15
José Rubén Ramírez-Iglesias, María Carolina Pérez-Gordones, Jesús Rafael Del Castillo, Alfredo Mijares, Gustavo Benaim, Marta Mendoza
The plasma membrane Ca2+ -ATPase (PMCA) from trypanosomatids lacks a classical calmodulin (CaM) binding domain, although CaM stimulated activities have been detected by biochemical assays. Recently we proposed that the Trypanosoma equiperdum CaM-sensitive PMCA (TePMCA) contains a potential 1-18 CaM-binding motif at the C-terminal region of the pump. In the present study, we evaluated the potential CaM-binding motifs using CaM from Trypanosoma cruzi and either the recombinant full length TePMCA C-terminal sequence (P14) or synthetic peptides comprising different regions of the C-terminal domain...
May 9, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29747818/glycan-chains-of-gangliosides-functional-ligands-for-tissue-lectins-siglecs-galectins
#16
Robert W Ledeen, Jürgen Kopitz, José Abad-Rodríguez, Hans-Joachim Gabius
Molecular signals on the cell surface are responsible for adhesion and communication. Of relevance in this respect, their chemical properties endow carbohydrates with the capacity to store a maximum of information in a minimum of space. One way to present glycans on the cell surface is their covalent conjugation to a ceramide anchor. Among the resulting glycosphingolipids, gangliosides are special due to the presence of at least one sialic acid in the glycan chains. Their spatial accessibility and the dynamic regulation of their profile are factors that argue in favor of a role of glycans of gangliosides as ligands (counterreceptors) for carbohydrate-binding proteins (lectins)...
2018: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29746580/characterization-and-mapping-of-leaf-rust-resistance-in-four-durum-wheat-cultivars
#17
Dhouha Kthiri, Alexander Loladze, P R MacLachlan, Amidou N'Diaye, Sean Walkowiak, Kirby Nilsen, Susanne Dreisigacker, Karim Ammar, Curtis J Pozniak
Widening the genetic basis of leaf rust resistance is a primary objective of the global durum wheat breeding effort at the International Wheat and Maize Improvement Center (CIMMYT). Breeding programs in North America are following suit, especially after the emergence of new races of Puccinia triticina such as BBG/BP and BBBQD in Mexico and the United States, respectively. This study was conducted to characterize and map previously undescribed genes for leaf rust resistance in durum wheat and to develop reliable molecular markers for marker-assisted breeding...
2018: PloS One
https://www.readbyqxmd.com/read/29735708/-staphylococcus-aureus-clumping-factor-a-is-a-force-sensitive-molecular-switch-that-activates-bacterial-adhesion
#18
Philippe Herman-Bausier, Cristina Labate, Aisling M Towell, Sylvie Derclaye, Joan A Geoghegan, Yves F Dufrêne
Clumping factor A (ClfA), a cell-wall-anchored protein from Staphylococcus aureus , is a virulence factor in various infections and facilitates the colonization of protein-coated biomaterials. ClfA promotes bacterial adhesion to the blood plasma protein fibrinogen (Fg) via molecular forces that have not been studied so far. A unique, yet poorly understood, feature of ClfA is its ability to favor adhesion to Fg at high shear stress. Unraveling the strength and dynamics of the ClfA-Fg interaction would help us better understand how S...
May 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29735293/anchor-negatively-regulates-bmp-signalling-to-control-drosophila-wing-development
#19
Xiao Chun Wang, Ziguang Liu, Li Hua Jin
G protein-coupled receptors play particularly important roles in many organisms. The novel Drosophila gene anchor is an orthologue of vertebrate GPR155. However, the roles of anchor in molecular functions and biological processes, especially in wing development, remain unknown. Knockdown of anchor resulted in an increased wing size and additional and thickened veins. These abnormal wing phenotypes were similar to those observed in BMP signalling gain-of-function experiments. We observed that the BMP signalling indicator p-Mad was significantly increased in wing discs in which anchor RNAi was induced in larvae and accumulated abnormally in intervein regions in pupae...
April 25, 2018: European Journal of Cell Biology
https://www.readbyqxmd.com/read/29734780/cpkc%C3%AE-modulated-sequential-reactivation-of-mtor-inhibited-autophagic-flux-in-neurons-exposed-to-oxygen-glucose-deprivation-reperfusion
#20
Rongrong Hua, Song Han, Nan Zhang, Qingqing Dai, Ting Liu, Junfa Li
We have reported that conventional protein kinase Cγ (cPKCγ)-modulated neuron-specific autophagy improved the neurological outcome of mice following ischemic stroke through the Akt-mechanistic target of rapamycin (mTOR) pathway. However, its detailed molecular mechanism remains unclear. In this study, primary cortical neurons from postnatal one-day-old C57BL/6J cPKCγ wild-type ( cPKCγ +/+ ) and knockout ( cPKCγ −/− ) mice suffering oxygen glucose deprivation/reperfusion (OGD/R) were used to simulate ischemia/reperfusion injury in vitro...
May 6, 2018: International Journal of Molecular Sciences
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