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https://www.readbyqxmd.com/read/28732182/stim1-regulates-enamel-mineralization-and-ameloblast-modulation
#1
Y Furukawa, N Haruyama, M Nikaido, M Nakanishi, N Ryu, M Oh-Hora, K Kuremoto, K Yoshizaki, Y Takano, I Takahashi
Loss-of-function mutations in the Ca(2+) release-activated Ca(2+) channel genes ORAI1 and STIM1 abolish store-operated Ca(2+) entry (SOCE) and result in ectodermal dysplasia with amelogenesis imperfecta. However, because of the limited availability of patient tissue, analyses of enamel mineralization or possible changes in ameloblast function or morphology have not been possible. Here, we generated mice with ectodermal tissue-specific deletion of Stim1 ( Stim1 cKO [conditional knockout]), Stim2 ( Stim2 cKO), and Stim1 and Stim2 ( Stim1/2 cKO) and analyzed their enamel phenotypes as compared with those of control ( Stim1/2(fl/fl)) animals...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28731314/desensitization-to-chemical-and-food-sensitivities-by-low-dose-immunotherapy-ascertained-by-provocation-neutralization-is-associated-with-reduced-influx-of-calcium-ions-into-lymphocytes
#2
Basant K Puri, John McLaren Howard, Jean A Monro
Background Food and chemical sensitivities have detrimental effects on health and the quality of life. The natural course of such sensitivities can potentially be altered through various types of allergen-specific immunotherapy, including low-dose immunotherapy. The molecular mechanism by which low-dose immunotherapy causes desensitization has not thus far been elucidated. While resting lymphocytes maintain a low cytosolic calcium ion concentration, antigen receptor signaling results in calcium ion influx, predominantly via store-operated calcium channels...
April 26, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28724541/stim1-and-stim2-cooperatively-regulate-mouse-neutrophil-store-operated-calcium-entry-and-cytokine-production
#3
Regina A Clemens, Joshua Chong, Derayvia Grimes, Yongmei Hu, Clifford A Lowell
Neutrophils are key effector cells of the innate immune system. Calcium-dependent signaling pathways initiated by store-operated calcium entry (SOCE) are known to regulate neutrophil activation, however the precise mechanism of this process remains unclear. STIM1 and STIM2 are calcium sensing molecules that link calcium depletion of the endoplasmic reticulum with opening of plasma membrane calcium channels. While a role for STIM1 in neutrophil SOCE and activation has been established, the function of STIM2 is unknown...
July 19, 2017: Blood
https://www.readbyqxmd.com/read/28720176/determination-of-polycyclic-aromatic-hydrocarbons-in-commercial-parenteral-formulations-and-medications-using-high-performance-liquid-chromatography-with-diode-array-detection
#4
Marcia M Barichello, Denise Bohrer, Carine Viana, Leandro M Carvalho, Paulo C Nascimento
HPLC coupled to UV diode array detection (DAD) is proposed for the determination of polycyclic aromatic hydrocarbons (PAHs) in pharmaceutical products for parenteral administration. Because rubber is a possible source of PAHs for these products, samples stored in containers with rubber parts were selected for the analysis. The basis for method optimization was EPA Method 8310, which determines 16 priority PAHs in ground water and wastewater by HPLC using both UV and fluorescence detection. Using DAD, two channels were selected for detection, with one operating at 254 nm for the detection of nine PAHs and the other at 225 nm for the detection of seven PAHs...
July 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28716825/efficiency-and-safety-of-crac-inhibitors-in-human-rheumatoid-arthritis-xenograft-models
#5
Shuang Liu, Hitoshi Hasegawa, Erika Takemasa, Yasuyuki Suzuki, Keizou Oka, Takeshi Kiyoi, Hiroyuki Takeda, Tomio Ogasawara, Tatsuya Sawasaki, Masaki Yasukawa, Kazutaka Maeyama
Store-operated Ca(2+) release-activated Ca(2+) (CRAC) channels are involved in the pathogenesis of rheumatoid arthritis (RA) and have been studied as therapeutic targets in the management of RA. We investigated the efficacy and safety of CRAC inhibitors, including a neutralizing Ab (hCRACM1-IgG) and YM-58483, in the treatment of RA. Patient-derived T cell and B cell activity was suppressed by hCRACM1-IgG as well as YM-58483. Systemically constant, s.c. infused CRAC inhibitors showed anti-inflammatory activity in a human-NOD/SCID xenograft RA model as well as protective effects against the destruction of cartilage and bone...
July 17, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28713161/functional-expression-of-the-ca-2-signaling-machinery-in-human-embryonic-stem-cells
#6
Ji-Jun Huang, Yi-Jie Wang, Min Zhang, Peng Zhang, He Liang, Hua-Jun Bai, Xiu-Jian Yu, Huang-Tian Yang
Emerging evidence suggests that Ca(2+) signals are important for the self-renewal and differentiation of human embryonic stem cells (hESCs). However, little is known about the physiological and pharmacological properties of the Ca(2+)-handling machinery in hESCs. In this study we used RT-PCR and Western blotting to analyze the expression profiles of genes encoding Ca(2+)-handling proteins; we also used confocal Ca(2+) imaging and pharmacological approaches to determine the contribution of the Ca(2+)-handling machinery to the regulation of Ca(2+) signaling in hESCs...
July 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28707074/presenilin-1-delta-e9-mutant-induces-stim1-driven-store-operated-calcium-channel-hyperactivation-in-hippocampal-neurons
#7
Maria Ryazantseva, Anna Goncharova, Kseniia Skobeleva, Maksim Erokhin, Axel Methner, Pavel Georgiev, Elena Kaznacheyeva
Presenilins regulate calcium homeostasis in the endoplasmic reticulum, and dysregulation of intracellular calcium has been implicated in the pathogenesis of Alzheimer disease. Elevated presenilin-1 (PS1) holoprotein levels have been detected in postmortem brains of patients carrying familial Alzheimer disease (FAD) PS1 mutations. This study examines the effect of the FAD presenilin mutant that lacks the ninth exon (PS1 ∆E9) and does not undergo endoproteolysis on store-operated calcium (SOC) entry. Significant enhancement of SOC channel activation was detected by electrophysiological measurements in hippocampal neurons with PS1 ∆E9 mutant expression...
July 13, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28701513/the-prion-protein-regulates-glutamate-mediated-ca-2-entry-and-mitochondrial-ca-2-accumulation-in-neurons
#8
Agnese De Mario, Caterina Peggion, Maria Lina Massimino, Francesca Viviani, Angela Castellani, Marta Giacomello, Dmitry Lim, Alessandro Bertoli, Maria Catia Sorgato
The prion protein, PrP(C), whose conformational misfolding originates deadly prions, has a still unclarified cellular function despite decades of intensive research. Following our recent finding that PrP(C) limits Ca(2+) entry via store-operated Ca(2+) channels in neurons, we investigated whether the protein could also control the activity of ionotropic glutamate receptors (iGluRs). To this end, we compared local Ca(2+) movements in primary cerebellar granule neurons and cortical neurons, expressing, or not, PrP(C), transduced with genetically encoded Ca(2+) probes...
July 12, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28698564/homer-binds-to-orai1-and-trpc-channels-in-the-neointima-and-regulates-vascular-smooth-muscle-cell-migration-and-proliferation
#9
Shuping Jia, Miguel Rodriguez, Arthur G Williams, Joseph P Yuan
The molecular components of store-operated Ca(2+) influx channels (SOCs) in proliferative and migratory vascular smooth muscle cells (VSMCs) are quite intricate with many channels contributing to SOCs. They include the Ca(2+)-selective Orai1 and members of the transient receptor potential canonical (TRPC) channels, which are activated by the endoplasmic reticulum Ca(2+) sensor STIM1. The scaffolding protein Homer assembles SOC complexes, but its role in VSMCs is not well understood. Here, we asked whether these SOC components and Homer1 are present in the same complex in VSMCs and how Homer1 contributes to VSMC SOCs, proliferation, and migration leading to neointima formation...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28697919/fatty-acid-translocase-promoted-hepatitis-b-virus-replication-by-upregulating-the-levels-of-hepatic-cytosolic-calcium
#10
Jian Huang, Lei Zhao, Ping Yang, Zhen Chen, Xiong Z Ruan, Ailong Huang, Ni Tang, Yaxi Chen
Hepatitis B virus (HBV) is designated a "metabolovirus" due to the intimate connection between the virus and host metabolism. The nutrition state of the host plays a relevant role in the severity of HBV infection. Metabolic syndrome (MS) is prone to increasing HBV DNA loads and accelerating the progression of liver disease in patients with chronic hepatitis B (CHB). Cluster of differentiation 36 (CD36), also named fatty acid translocase, is known to facilitate long-chain fatty acid uptake and contribute to the development of MS...
July 8, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28694529/the-production-of-fibroblast-growth-factor-23-is-controlled-by-tgf-%C3%AE-2
#11
Martina Feger, Philipp Hase, Bingbing Zhang, Frank Hirche, Philipp Glosse, Florian Lang, Michael Föller
Transforming growth factor-β (TGF-β) is a cytokine produced by many cell types and implicated in cell growth, differentiation, apoptosis, and inflammation. It stimulates store-operated calcium entry (SOCE) through the calcium release-activated calcium (CRAC) channel Orai1/Stim1 in endometrial Ishikawa cells. Bone cells generate fibroblast growth factor (FGF) 23, which inhibits renal phosphate reabsorption and 1,25(OH)2D3 formation in concert with its co-receptor Klotho. Moreover, Klotho and FGF23 counteract aging and age-related clinical conditions...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28677119/atp-evokes-ca-2-signals-in-cultured-foetal-human-cortical-astrocytes-entirely-through-g-protein-coupled-p2y-receptors
#12
Margit S Muller, Colin W Taylor
Extracellular ATP plays important roles in coordinating the activities of astrocytes and neurons, and aberrant signalling is associated with neurodegenerative diseases. In rodents, ATP stimulates opening of Ca(2+) -permeable channels formed by P2X receptor subunits in the plasma membrane. It is widely assumed, but not verified, that P2X receptors also evoke Ca(2+) signals in human astrocytes. Here we directly assess this hypothesis. We showed that cultured foetal cortical human astrocytes express mRNA for several P2X receptor subunits (P2X4 , P2X5 , P2X6 ) and G-protein-coupled P2Y receptors (P2Y1 , P2Y2 , P2Y6 , P2Y11 )...
July 5, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28658523/effect-of-tetramethylpyrazine-tmp-on-ca-2-signal-transduction-and-cell-viability-in-a-model-of-renal-tubular-cells
#13
Yi-Chien Fang, Chiang-Ting Chou, Wei-Zhe Liang, Chun-Chi Kuo, Shu-Shong Hsu, Jue-Long Wang, Chung-Ren Jan
Tetramethylpyrazine (TMP) is a compound purified from herb. Its effect on Ca(2+) concentrations ([Ca(2+) ]i ) in renal cells is unclear. This study examined whether TMP altered Ca(2+) signaling in Madin-Darby canine kidney (MDCK) cells. TMP at 100-800 μM induced [Ca(2+) ]i rises, which were reduced by Ca(2+) removal. TMP induced Mn(2+) influx implicating Ca(2+) entry. TMP-induced Ca(2+) entry was inhibited by 30% by modulators of protein kinase C (PKC) and store-operated Ca(2+) channels. Treatment with the endoplasmic reticulum Ca(2+) pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) inhibited 93% of TMP-evoked [Ca(2+) ]i rises...
June 28, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28656279/silencing-of-%C3%AE-1-integrin-regulates-airway-remodeling-by-regulating-the-transcription-of-soce%C3%A2-associated-genes-in-asthmatic-mice
#14
Chen Qiu, Wenwen Liu, Fei Shi, Mengjie Fen, Lili Ren, Hui Qi
The incidence of asthma is increasing globally; however, current treatments are only able to cure a certain proportion of patients. There is an urgent need to develop novel therapies. β1 integrin serves a role in the pathophysiology of asthma through the development of airway remodeling. The aim of the present study was to investigate silencing of the β1 integrin gene in pre‑clinical models of allergic asthma. BALB/c mice were sensitized with ovalbumin through intraperitoneal injection and repeated aerosolized ovalbumin...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28653722/porous-pdms-structures-for-the-storage-and-release-of-aqueous-solutions-into-fluidic-environments
#15
Peter Thurgood, Sara Baratchi, Crispin Szydzik, Arnan Mitchell, Khashayar Khoshmanesh
Typical microfluidic systems take advantage of multiple storage reservoirs, pumps and valves for the storage, driving and release of buffers and other reagents. However, the fabrication, integration, and operation of such components can be difficult. In particular, the reliance of such components on external off-chip equipment limits their utility for creating self-sufficient, stand-alone microfluidic systems. Here, we demonstrate a porous sponge made of polydimethylsiloxane (PDMS), which is fabricated by templating microscale water droplets using a T-junction microfluidic structure...
June 27, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28637791/store-operated-calcium-entry-suppressed-tgf%C3%AE-1-smad3-signaling-pathway-in-glomerular-mesangial-cells
#16
Sarika Chaudhari, Weizu Li, Yanxia Wang, Hui Jiang, Yuhong Ma, Mark E Davis, Jonathan E Zuckerman, Rong Ma
Our previous study demonstrated that the abundance of extracellular matrix proteins was suppressed by store-operated Ca2+ entry in mesangial cells (MCs). The present study was conducted to investigate the underlying mechanism focused on the transforming growth factor beta 1 (TGFβ1) - Smad3 pathway, a critical pathway for ECM expansion in diabetic kidneys. We hypothesized that SOCE suppressed ECM protein expression by inhibiting this pathway in MCs. In cultured human MCs, we observed that TGFβ1 (5 ng/ml for 15 hours) significantly increased Smad3 phosphorylation as evaluated by immunoblot...
June 21, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28588083/science-signaling-podcast-for-6-june-2017-calcium-signaling-and-dry-mouth
#17
Indu Ambudkar, Annalisa M VanHook
This Podcast features a conversation with Indu Ambudkar, senior author of a Research Resource that appears in the 6 June 2017 issue of Science Signaling, about how activation of the cation channel TRPM2 is involved in radiation-induced dry mouth. Patients who receive radiation therapy for head and neck cancers often develop dry mouth as a side effect, and this condition is frequently permanent. Radiation does not kill cells in the salivary gland, yet it causes the acinar cells of the gland to reduce the amount of saliva they secrete...
June 6, 2017: Science Signaling
https://www.readbyqxmd.com/read/28578393/mti-101-treatment-inducing-activation-of-stim1-and-trpc1-expression-is-a-determinant-of-response-in-multiple-myeloma
#18
Michael F Emmons, Nagaraju Anreddy, Javier Cuevas, Kayla Steinberger, Shengyu Yang, Mark McLaughlin, Ariosto Silva, Lori A Hazlehurst
The emergence of drug resistance continues to be a major hurdle towards improving patient outcomes for the treatment of Multiple Myeloma. MTI-101 is a first-in-class peptidomimetic that binds a CD44/ITGA4 containing complex and triggers necrotic cell death in multiple myeloma cell lines. In this report, we show that acquisition of resistance to MTI-101 correlates with changes in expression of genes predicted to attenuate Ca(2+) flux. Consistent with the acquired resistant genotype, MTI-101 treatment induces a rapid and robust increase in intracellular Ca(2+) levels in the parental cells; a finding that was attenuated in the acquired drug resistant cell line...
June 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28578345/calcium-channel-opening-rather-than-the-release-of-atp-causes-the-apoptosis-of-osteoblasts-induced-by-overloaded-mechanical-stimulation
#19
Lu Liu, Hui Li, Ying Cui, Ruixin Li, Fan Meng, Zi Ye, Xizheng Zhang
BACKGROUND: Stress fracture is one of the most common overuse injuries in athletes. Overloaded mechanical stimulation is an important factor affecting stress fractures, but the mechanism is unclear. METHODS: MC3T3-E1 cells and a polycaprolactone (PCL) scaffold were co-cultured, and finite element analysis (FEA) was used to analyze the load-carrying capability. Cell proliferation was investigated with CCK-8 assays. An alkaline phosphatase (AKP) activity assay was used to evaluate cell differentiation...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28566634/effect-of-docosahexaenoic-acid-on-voltage-independent-ca-2-entry-pathways-in-cultured-vascular-smooth-muscle-cells-stimulated-with-5-hydroxytryptamine
#20
Takuji Machida, Akina Onoguchi, Kenji Iizuka, Sayuri Ishibashi, Mikiko Yutani, Masahiko Hirafuji
We previously reported that docosahexaenoic acid (DHA) inhibits an increase in intracellular Ca(2+) concentration ([Ca(2+)]i) in cultured rat vascular smooth muscle cells (VSMCs) through a mechanism involving mainly voltage-dependent Ca(2+) channels; however, the effect of DHA on voltage-independent pathways, such as store-operated and receptor-operated Ca(2+) entry, and Ca(2+) entry through Na(+)/Ca(2+) exchanger (NCX), has not been clarified. In the present study, we investigated the effect of DHA treatment on the expression of transient receptor potential canonical (TRPC) channels, capacitative Ca(2+) entry, and Ca(2+) entry through NCX in rat cultured VSMCs stimulated with 5-hydroxytryptamine (5-HT)...
2017: Biological & Pharmaceutical Bulletin
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